



@article {2214,
	title = {Mesenchymal stem cell recruitment by stromal derived factor-1-delivery systems based on chitosan/poly(γ-glutamic acid) polyelectrolyte complexes},
	journal = {European Cells and MaterialsEur. Cells and Mater.},
	volume = {23},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Gon{\c c}alves, R. M.; INEB, R. Campo Alegre, 823, Porto 4150-180, Portugal; email: raquelg@ineb.up.ptChemicals/CAS: chitosan, 9012-76-4; polyglutamic acid, 24991-23-9, 25513-46-6; Biocompatible Materials; CXCL12 protein, human; Chemokine CXCL12; Chitosan, 9012-76-4; Membranes, Artificial; Polyglutamic Acid, 25513-46-6; poly(gamma-glutamic acid)References: Almeida, C.R., Vasconcelos, D.P., Gon{\c c}alves, R., Barbosa, M.A., Enhanced me s enchymal stromal cell recruitment via Natural Killer cells by incorporation of inflammatory signals in biomaterials (2012) J R Soc Interface, 9, pp. 261-271;Amersham, Iodine-125. A guide to radioiodination techniques (1993) Amersham Life Science, , (I.-A.g.t.r. Techniques, Ed). Amersham, Little Chalfort, UK; 
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},
	month = {2012///},
	pages = {249 - 261},
	abstract = {Human mesenchymal stem cells (hMSCs) have an enormous potential for tissue engineering and cell-based therapies. With a potential of differentiation into multiple lineages and immune-suppression, these cells play a key role in tissue remodelling and regeneration. Here a method of hMSC recruitment is described, based on the incorporation of a chemokine in Chitosan (Ch)/ Poly(γ-glutamic acid) (γ-PGA) complexes. Ch is a nontoxic, cationic polysaccharide widely investigated. γ-PGA is a hydrophilic, non-toxic, biodegradable and negatively charged poly-amino acid. Ch and γ-PGA, being oppositely charged, can be combined through electrostatic interactions. These biocompatible structures can be used as carriers for active substances and can be easily modulated in order to control the delivery of drugs, proteins, DNA, etc. Using the layer-by-layer method, Ch and γ-PGA were assembled into polyelectrolyte multilayers films (PEMs) with thickness of 120 nm. The chemokine stromal-derived factor-1 (SDF-1) was incorporated in these complexes and was continuously released during 120 h. The method of SDF-1 incorporation is of crucial importance for polymers assembly into PEMs and for the release kinetics of this chemokine. The Ch/γ-PGA PEMs with SDF-1 were able to recruit hMSCs, increasing the cell migration up to 6 fold to a maximum of 16.2 {\textpm} 4.9 cells/mm                        2. The controlled release of SDF-1 would be of great therapeutic value in the process of hMSC homing to injured tissues. This is the first study suggesting Ch/γ-PGA PEMs as SDF-1 reservoirs to recruit hMSCs, describing an efficient method of chemokine incorporation that allows a sustained released up to 5 days and that can be easily scaled-up.
},
	keywords = {adsorption kinetics, article, artificial membrane, Biocompatible Materials, biomaterial, cell differentiation, cell migration, cell motion, Cell Movement, Cell recruitment, chemistry, Chemokine CXCL12, chitosan, controlled study, CXCL12 protein, human, cytology, drug adsorption, drug delivery system, Drug Delivery Systems, drug derivative, drug effect, ellipsometry, enzyme linked immunosorbent assay, human, human cell, human tissue, Humans, in vitro study, isotope labeling, Layer-by-layer, Membranes, Artificial, Mesenchymal stem cell, Mesenchymal Stem Cells, metabolism, methodology, poly(gamma glutamic acid), poly(gamma-glutamic acid), Poly(γ-glutamic acid), polyglutamic acid, Protein delivery, static electricity, stromal cell derived factor 1, sustained drug release},
	isbn = {14732262 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864582297\&partnerID=40\&md5=2fe4a066582cc820d2a8f9dd64fde21b},
	author = {Gon{\c c}alves, R.M. and Antunes, J.C. and Barbosa, M.A.}
}
@article {2287,
	title = {Anti-adhesion and antiproliferative cellulose triacetate membrane for prevention of biomaterial-centred infections associated with Staphylococcus epidermidis},
	journal = {International Journal of Antimicrobial AgentsInt. J. Antimicrob. Agents},
	volume = {35},
	year = {2010},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: IAAGEdoi: 10.1016/j.ijantimicag.2009.09.017PubMed ID: 19942411Language of Original Document: EnglishCorrespondence Address: Extremina, C.I.; REQUIMTE, Faculdade de Farm{\'a}cia, Universidade do Porto, Rua An{\'\i}bal Cunha 164, 4050-047 Porto, Portugal; email: extremina@gmail.comChemicals/CAS: cellulose triacetate, 9012-09-3; cilastatin plus imipenem, 92309-29-0; Anti-Bacterial Agents; Biocompatible Materials; Cellulose, 9004-34-6; Imipenem, 74431-23-5; cellulose triacetate, 9012-09-3Tradenames: tienam, Merck Sharp and Dohme, GermanyManufacturers: Eastman Kodak, Netherlands; Merck Sharp and Dohme, GermanyReferences: Darouiche, R.O., Treatment of infections associated with surgical implants (2004) N Engl J Med, 350, pp. 1422-1429;Fonseca, A.P., Extremina, C., Fonseca, A.F., Sousa, J.C., Effect of subinhibitory concentration of piperacillin/tazobactam on Pseudomonas aeruginosa (2004) J Med Microbiol, 53, pp. 903-910; 
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},
	month = {2010///},
	pages = {164 - 168},
	abstract = {The initial step in preventing biomaterial-associated infections consists of preventing bacterial adhesion to the device surface. One possible approach is the design of antibiotic-releasing biomaterials. Cellulose triacetate (CTA) membranes with the antibiotic imipenem (IPM) entrapped (CTA-IPM) were prepared. The material was characterised in terms of surface morphology by scanning electron microscopy, surface free energy of interaction and X-ray photoelectron spectroscopy (XPS). Antibiotic release studies were also performed. In vitro adhesion of Staphylococcus epidermidis RP62A to CTA-IPM was investigated using a modified microtitre plate assay, and the antibacterial activity of the CTA-IPM membrane was assessed by a modified Kirby-Bauer test, which showed effective entrapment of the antibiotic as confirmed by XPS and hydrophilicity assays. Release studies showed that this drug-polymer conjugate serves as an adequate reservoir for sustained release of IPM over a period of 71 h at an effective bacteriostatic concentration. Moreover, bacterial adhesion tests showed a statistically significant decrease in the adhesion of S. epidermidis RP62A to CTA-IPM compared with its adhesion to CTA alone. The present innovative approach is capable of providing a membrane with anti-adhesive and antiproliferative properties, thus encouraging in vivo studies to provide a better simulation of the clinical situation. {\textcopyright} 2009 Elsevier B.V. and the International Society of Chemotherapy.},
	keywords = {Adhesion, Anti-Bacterial Agents, antibacterial activity, Antimicrobial agents, article, Bacterial adhesion, bacterial growth, bacteriostasis, bacterium adherence, Biocompatible Materials, biomaterial, Cellulose, Cellulose triacetate, cilastatin plus imipenem, controlled study, device infection, drug screening, Equipment and Supplies, growth inhibition, Humans, hydrophilicity, Imipenem, in vitro study, infection prevention, Membranes, Microbial Sensitivity Tests, microtiter plate assay, nonhuman, priority journal, Prosthesis-Related Infections, Scanning electron microscopy, Staphylococcus epidermidis, surface property, sustained drug release, X ray photoelectron spectroscopy},
	isbn = {09248579 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-72649093658\&partnerID=40\&md5=3fbe3d7e15447e7ae40bb5fdd3f63b61},
	author = {Extremina, C.I. and Fonseca, A.F.d. and Granja, P.L. and Fonseca, A.P.}
}
@article {2377,
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	year = {2007},
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},
	month = {2007///},
	pages = {994 - 1004},
	abstract = {Severe periodontitis treatment, where massive alveolar bone loss occurs, involves bone defect filling and intensive systemic log-term antibiotics administration. This study aims at developing novel injectable drug delivery systems (nanohydroxyapatite microspheres) with the drug releasing capability for periodontitis treatment and simultaneously initiating the osteointegration process. Materials were characterized by XRD, SEM, inverted stand optical microscope analysis, and mercury porosimetry method. Amoxicillin, amoxicillin + clavulanic acid, and erythromycin were the antibiotics used. Release properties during 28 days from the hydroxyapatite (HA) granules, and two types of nanoHA microspheres were investigated. Biocompatibility was assessed by cytotoxicity assays. HA granules were inadequate, releasing all antibiotic during the first hours. The concentration of antibiotics released in the first days from HA-2 was higher than from HA-1 microspheres, because of the increased porosity and surface area. The release profiles (fast initial release followed by long-term sustained release) of effective doses of antibiotics make these systems good alternatives for antibiotics delivery. Osteoblasts proliferated well on both types of microspheres, being cell growth enhanced in the presence of antibiotics. Erythromycin presented the most beneficial effect. Combining the sustained antibiotic release with the osteoconduction, resorbability, and potential use as injectable bone filling material of porous HA microspheres, these systems provided a forth fold beneficial effect. {\textcopyright} 2007 Wiley Periodicals, Inc.},
	keywords = {alveolar bone, amoxicillin, amoxicillin plus clavulanic acid, Anti-Bacterial Agents, Antibacterial, antibiotic agent, antibiotic resistance, Antibiotics administration, antimicrobial activity, article, Bacteria, Cell Communication, Cell Death, cell growth, cell kinetics, Cell Line, cell proliferation, clindamycin, concentration response, controlled study, Cytotoxicity, Drug delivery, drug delivery system, Drug Delivery Systems, Drug release, Durapatite, erythromycin, Escherichia coli, human, human cell, Humans, hydroxyapatite, Kinetics, materials testing, metronidazole, Microbial Sensitivity Tests, microsphere, Microspheres, minocycline, Nanohydroxyapatite, nanoparticle, nanoparticles, nonhuman, Optical microscope analysis, osteoblast, Osteoblasts, osteolysis, periodontitis, porosity, Staphylococcus aureus, surface property, sustained drug release, Tetracycline, unclassified drug, X ray diffraction},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34249689569\&partnerID=40\&md5=5474dac8549e9cd037a96eba13ac9605},
	author = {Ferraz, M.P. and Mateus, A.Y. and Sousa, J.C. and Monteiro, F.J.}
}
