



@article {2205,
	title = {Adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa onto nanohydroxyapatite as a bone regeneration material},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {100 A},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.34139PubMed ID: 22489063Language of Original Document: EnglishCorrespondence Address: Grenho, L.; INEB, Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua Campo Alegre, 823, 4150-180 Porto, Portugal; email: liliana.grenho@ineb.up.ptChemicals/CAS: Biocompatible Materials; Durapatite, 1306-06-5References: Darley, E.S.R., MacGowan, A.P., Antibiotic treatment of Gram-positive bone and joint infections (2004) J Antimicrob Chemother, 53, pp. 928-935;Charville, G.W., Hetrick, E.M., Geer, C.B., Schoenfisch, M.H., Reduced bacterial adhesion to fibrinogen-coated substrates via nitric oxide release (2008) Biomaterials, 29, pp. 4039-4044; 
Katsikogianni, M., Amanatides, E., Mataras, D., Missirlis, Y.F., Staphylococcus epidermidis adhesion to He, He/O(2) plasma treated PET films and aged materials: Contributions of surface free energy and shear rate (2008) Colloids Surf B Biointerfaces, 65, pp. 257-268; 
Katsikogianni, M., Missirlis, Y.F., Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria-material interactions (2004) Eur Cell Mater, 8, pp. 37-57; 
Poelstra, K.A., Barekzi, N.A., Rediske, A.M., Felts, A.G., Slunt, J.B., Grainger, D.W., Prophylactic treatment of gram-positive and gram-negative abdominal implant infections using locally delivered polyclonal antibodies (2002) J Biomed Mater Res, 60, pp. 206-215; 
Pascual, A., Pathogenesis of catheter-related infections: Lessons for new designs (2002) Clin Microbiol Infect, 8, pp. 256-264; 
Katsikogianni, M., Spiliopoulou, I., Dowling, D.P., Missirlis, Y.F., Adhesion of slime producing Staphylococcus epidermidis strains to PVC and diamond-like carbon/silver/fluorinated coatings (2006) J Mater Sci Mater Med, 17, pp. 679-689; 
Donlan, R.M., Role of biofilms in antimicrobial resistance (2000) Asaio J, 46, pp. S47-S52; 
Donlan, R.M., Biofilms and device-associated infections (2001) Emerg Infect Dis, 7, pp. 277-281; 
Donlan, R.M., Biofilms: Microbial life on surfaces (2002) Emerg Infect Dis, 8, pp. 881-890; 
Ferraz, M.P., Monteiro, F.J., Giao, D., Leon, B., Gonzalez, P., Liste, S., Serra, J., Perez-Amor, M., CaO-P 2O 5 glass-hydroxyapatite thin films obtained by laser ablation: Characterisation and in vitro bioactivity evaluation (2004) Bioceramics, 16, pp. 347-350; 
Ferraz, M.P., Monteiro, F.J., Manuel, C.D., Hydroxyapatite nanoparticles: A review of preparation methodologies (2004) J Appl Biomater Biomech, 2, pp. 74-80; 
Mateus, A.Y.P., Barrias, C.C., Ribeiro, C.C., Ferraz, M.P., Monteiro, F.J., Comparative study of nanohydroxyapatite microspheres for medical applications (2008) J Biomed Mater Res A, 86, pp. 483-493; 
Teixeira, S., Ferraz, M.P., Monteiro, F.J., Biocompatibility of highly macroporous ceramic scaffolds: Cell adhesion and morphology studies (2008) J Mater Sci Mater Med, 19, pp. 855-859; 
Ferraz, M.P., Mateus, A.Y., Sousa, J.C., Monteiro, F.J., Nano-hydroxyapatite microspheres as delivery system for antibiotics: Release kinetics, antimicrobial activity and interaction with osteoblasts (2007) J Biomed Mater Res A, 81, pp. 994-1004; 
Oliveira, G.M., Ferraz, M.P., Gonz{\'a}lez, P., Serra, J., Leon, B., P{\`e}rez-Amor, M., Monteiro, F.J., PLD bioactive ceramic films: The influence of CaO-P2O5 glass additions to hydroxyapatite on the proliferation and morphology of osteblastic like-cells (2008) J Mater Sci Mater Med, 19, pp. 1775-1785; 
Ribeiro, N., Sousa, S.R., Monteiro, F.J., Influence of crystallite size of nanophased hydroxyapatite on fibronectin and osteonectin adsorption and on MC3T3-E1 osteoblast adhesion and morphology (2010) J Colloid Interface Sci, 351, pp. 398-406; 
Gadelmawla, E.S., Koura, M.M., Maksoud, T.M.A., Elewa, I.M., Soliman, H.H., Roughness parameters (2002) J Mater Proc Technol, 123, pp. 133-145; 
Campoccia, D., Montanaro, L., Arciola, C.R., The significance of infection related to orthopedic devices and issues of antibiotic resistance (2006) Biomaterials, 27, pp. 2331-2339; 
Muller, R., Groger, G., Hiller, K.A., Schmalz, G., Ruhl, S., Fluorescence-based bacterial overlay method for simultaneous in situ quantification of surface-attached bacteria (2007) Appl Environ Microbiol, 73, pp. 2653-2660; 
Boyd, R.D., Verran, J., Jones, M.V., Bhakoo, M., Use of the atomic force microscope to determine the effect of substratum surface topography on bacterial adhesion (2002) Langmuir, 18, pp. 2343-2346; 
Fonseca, A.P., Granja, P.L., Nogueira, J.A., Oliveira, D.R., Barbosa, M.A., Staphylococcus epidermidis RP62A adhesion to chemically modified cellulose derivatives (2001) J Mater Sci Mater Med, 12, pp. 543-548; 
Papaioannou, W., Quirynen, M., Vansteenberghe, D., The influence of periodontitis on the subgingival flora around implants in partially edentulous patients (1996) Clin Oral Implants Res, 7, pp. 405-409; 
Kamusewitz, H., Possart, W., Wetting and scanning force microscopy on rough polymer surfaces: Wenzel{\textquoteright}s roughness factor and the thermodynamic contact angle (2003) Appl Phys A Mater Sci Proc, 76, pp. 899-902; 
Katsikogianni, M.G., Missirlis, Y.F., Interactions of bacteria with specific biomaterial surface chemistries under flow conditions (2010) Acta Biomater, 6, pp. 1107-1118; 
Bazaka, K., Jacob, M.V., Crawford, R.J., Ivanova, E.P., Plasma-assisted surface modification of organic biopolymers to prevent bacterial attachment (2011) Acta Biomater, 7, pp. 2015-2028; 
Gotz, F., Staphylococcus and biofilms (2002) Mol Microbiol, 43, pp. 1367-1378
},
	month = {2012///},
	pages = {1823 - 1830},
	abstract = {In orthopedics due to the enormous number of surgical procedures involving invasive implant biomaterials, infections have a huge impact in terms of morbidity, mortality, and medical costs. In this study the initial adhesion of several strains namely Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa, to nanohydroxyapatite, previously heat-treated at 725{\textdegree}C and 1000{\textdegree}C was assessed. Adherent cells were evaluated by scanning electron microscopy and quantified by confocal laser scanning microscopy and as colony forming units after being released by sonication. The wettability and roughness of samples surfaces were assessed by contact angle measurements and atomic force microscopy, respectively. Nanohydroxyapatite heat-treated at 1000{\textdegree}C appeared to be more resistant to bacterial adhesion, over time, in five of the six tested strains while the clinical strains isolated from orthopedic infections presented superior ability to adhere, as well as better capacity to produce slime. The increase in materials sintering temperature resulted in increased hydrophobicity and roughness; however, other surface features such as the decrease in surface area and on porosity as well as the decrease on zeta potential may be the aspects that contributed to a lower bacterial adhesion on the materials sintered at 1000{\textdegree}C. {\textcopyright} 2012 Wiley Periodicals, Inc.},
	keywords = {Adherent cells, Adhesion, article, Atomic force microscopy, Bacteria, Bacterial adhesion, bacterium adherence, Biocompatible Materials, Biological materials, biomaterial, Biomaterials, Bone, bone infection, Bone Regeneration, Bone regeneration materials, Clinical strains, Colony Count, Microbial, colony forming unit, Colony forming units, confocal laser microscopy, Confocal laser scanning microscopy, confocal microscopy, Contact angle, Durapatite, hydrophobicity, Microscopy, Confocal, Microscopy, Electron, Scanning, Nano-hydroxyapatite, Nanohydroxyapatite, nanoparticles, nonhuman, porosity, Pseudomonas aeruginosa, Scanning electron microscopy, Sintering, Sintering temperatures, Staphylococcus aureus, Staphylococcus epidermidis, Surface area, Surface feature, surface property, Surface roughness, Surgical procedures, temperature, ultrasound, unclassified drug, wettability, Zeta potential},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84861570738\&partnerID=40\&md5=84186d33133ff6c5280db80ec9fcb12d},
	author = {Grenho, L. and Manso, M.C. and Monteiro, F.J. and Ferraz, M.P.}
}
@article {2203,
	title = {Biosynthesis of highly pure poly-γ-glutamic acid for biomedical applications},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {23},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/s10856-012-4639-xPubMed ID: 22532096Language of Original Document: EnglishCorrespondence Address: Pereira, C.L.; INEB-Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: Catarina.pereira@ineb.up.ptChemicals/CAS: chitosan, 9012-76-4; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; polyglutamic acid, 24991-23-9, 25513-46-6; Biocompatible Materials; Polyglutamic Acid, 25513-46-6; poly(gamma-glutamic acid)References: Ratner, B.D., Hoffman, A.S., Shoen, F.J., Lemons, J.E., (2004) Natural Materials. Biomaterials Science-an Introduction to Materials in Medicine, , 2nd ed. San Diego, CA: Academic Press;Buescher, J.M., Margaritis, A., Microbial biosynthesis of polyglutamic acid biopolymer and applications in the biopharmaceutical, biomedical and food industries (2007) Critical Reviews in Biotechnology, 27 (1), pp. 1-19. , DOI 10.1080/07388550601166458, PII 773236840; 
Kang, H.-S., Park, S.-H., Lee, Y.-G., Son, T.-I., Polyelectrolyte complex hydrogel composed of chitosan and poly(γ-glutamic acid) for biological application: Preparation, physical properties, and cytocompatibility (2007) Journal of Applied Polymer Science, 103 (1), pp. 386-394. , DOI 10.1002/app.24623; 
Lin, Y.-H., Chung, C.-K., Chen, C.-T., Liang, H.-F., Chen, S.-C., Sung, H.-W., Preparation of nanoparticles composed of chitosan/poly-γ-glutamic acid and evaluation of their permeability through Caco-2 cells (2005) Biomacromolecules, 6 (2), pp. 1104-1112. , DOI 10.1021/bm049312a; 
Sanda, F., Fujiyama, T., Endo, T., Chemical synthesis of polygamma-glutamic acid by polycondensation of gamma-glutamic acid dimer: Synthesis and reaction of poly gamma-glutamic acid methyl ester (2000) J Polym Sci, Part A: Polym Chem, 39, pp. 732-741; 
Ashiuchi, M., Shimanouchi, K., Nakamura, H., Kamei, T., Soda, K., Park, C., Sung, M.-H., Misono, H., Enzymatic synthesis of high-molecular-mass poly-γ-glutamate and regulation of its stereochemistry (2004) Applied and Environmental Microbiology, 70 (7), pp. 4249-4255. , DOI 10.1128/AEM.70.7.4249-4255.2004; 
Shih, I.-L., Van, Y.-T., The production of poly-(γ-glutamic acid) from microorganisms and its various applications (2001) Bioresource Technology, 79 (3), pp. 207-225. , DOI 10.1016/S0960-8524(01)00074-8, PII S0960852401000748; 
Prodhomme, E.J.F., Tutt, A.L., Glennie, M.J., Bugg, T.D.H., Multivalent Conjugates of Poly-γ-D-glutamic Acid from Bacillus licheniformis with Antibody F(ab{\textquoteright}) and Glycopeptide Ligands (2003) Bioconjugate Chemistry, 14 (6), pp. 1148-1155. , DOI 10.1021/bc020019m; 
Schneerson, R., Kubler-Kielb, J., Liu, T.-Y., Dai, Z.-D., Leppla, S.H., Yergey, A., Backlund, P., Robbins, J.B., Poly(γ-D-glutamic acid) protein conjugates induce IgG antibodies in mice to the capsule of Bacillus anthracis: A potential addition to the anthrax vaccine (2003) Proceedings of the National Academy of Sciences of the United States of America, 100 (15), pp. 8945-8950. , DOI 10.1073/pnas.1633512100; 
Edde, B., Rossier, J., Le Caer, J., Desbruyeres, E., Gros, F., Denoulet, P., Posttranslational glutamylation of alpha-tubulin (1990) Science, 247 (4938), pp. 83-85; 
Sung, M.-H., Park, C., Kim, C.-J., Poo, H., Soda, K., Ashiuchi, M., Natural and edible biopolymer poly-γ-glutamic acid: Synthesis, production, and applications (2005) Chemical Record, 5 (6), pp. 352-366. , DOI 10.1002/tcr.20061; 
Kunioka, M., Biosynthesis and chemical reactions of poly(amino acid)s from microorganisms (1997) Applied Microbiology and Biotechnology, 47 (5), pp. 469-475. , DOI 10.1007/s002530050958; 
Ashiuchi, M., Soda, K., Misono, H., A poly-γ-glutamate synthetic system of Bacillus subtilis IFO 3336: Gene cloning and biochemical analysis of poly-γ-glutamate produced by Escherichia coli clone cells (1999) Biochemical and Biophysical Research Communications, 263 (1), pp. 6-12. , DOI 10.1006/bbrc.1999.1298; 
Park, C., Choi, J.-C., Choi, Y.-H., Nakamura, H., Shimanouchi, K., Horiuchi, T., Misono, H., Sung, M.-H., Synthesis of super-high-molecular-weight poly-γ-glutamic acid by Bacillus subtilis subsp. chungkookjang (2005) Journal of Molecular Catalysis B: Enzymatic, 35 (4-6), pp. 128-133. , DOI 10.1016/j.molcatb.2005.06.007; 
Goto, A., Kunioka, M., Biosynthesis and hydrolysis of poly (c-glutamic acid) from Bacillus subtilis IFO3335 (1992) Biosci Biotechnol Biochem, 56 (7), pp. 1031-1035; 
Kubota, H., Matsunobu, T., Uotani, K., Takebe, H., Satoh, A., Tanaka, T., Production of poly(c-glutamic acid) by Bacillus subtilis F-2-01 (1993) Biosci Biotechnol Biochem, 57 (7), pp. 1212-1213; 
Kunioka, M., Goto, A., Biosynthesis of poly(γ-glutamic acid) from L-glutamic acid, citric acid, and ammonium sulfate in Bacillus subtilis IFO3335 (1994) Applied Microbiology and Biotechnology, 40 (6), pp. 867-872. , DOI 10.1007/s002530050082; 
Ashiuchi, M., Misono, H., Biochemistry and molecular genetics of poly-γ-glutamate synthesis (2002) Applied Microbiology and Biotechnology, 59 (1), pp. 9-14. , DOI 10.1007/s00253-002-0984-x; 
Hsieh, C.-Y., Tsai, S.-P., Wang, D.-M., Chang, Y.-N., Hsieh, H.-J., Preparation of γ-PGA/chitosan composite tissue engineering matrices (2005) Biomaterials, 26 (28), pp. 5617-5623. , DOI 10.1016/j.biomaterials.2005.02.012, PII S0142961205001614; 
Richard, A., Margaritis, A., Rheology, oxygen transfer, and molecular weight characteristics of poly(glutamic acid) fermentation by Bacillus subtilis (2003) Biotechnology and Bioengineering, 82 (3), pp. 299-305. , DOI 10.1002/bit.10568; 
Peng, S.-F., Yang, M.-J., Su, C.-J., Chen, H.-J., Lee, P.-W., Wei, M.-C., Effects of incorporation of poly(gamma-glutamic acid) in chitosan/DNA complex nanoparticles on cellular uptake and transfection efficiency (2009) Biomaterials, 30, pp. 1797-1808; 
Lin, Y.-H., Mi, F.-L., Chen, C.-T., Chang, W.-C., Peng, S.-F., Liang, H.-F., Sung, H.-W., Preparation and characterization of nanoparticles shelled with chitosan for oral insulin delivery (2007) Biomacromolecules, 8 (1), pp. 146-152. , DOI 10.1021/bm0607776; 
Lee, P.-W., Peng, S.-F., Su, C.-J., Mi, F.-L., Chen, H.-L., Wei, M.-C., Lin, H.-J., Sung, H.-W., The use of biodegradable polymeric nanoparticles in combination with a low-pressure gene gun for transdermal DNA delivery (2008) Biomaterials, 29 (6), pp. 742-751. , DOI 10.1016/j.biomaterials.2007.10.034, PII S014296120700837X; 
Hajdu, I., Bodn{\'a}r, M., Filipcsei, G., Hartmann, J., Dar{\'o}czi, L., Zr{\'\i}nyi, M., Nanoparticles prepared by self-assembly of Chitosan and poly-γ-glutamic acid (2008) Colloid Polym Sci, 286 (3), pp. 343-350; 
Keresztessy, Z., Bodn{\'a}r, M., Ber, E., Hajdu, I., Zhang, M., Hartmann, J., Self-assembling chitosan/poly-γ-glutamic acid nanoparticles for targeted drug delivery (2009) Colloid Polym Sci, 287 (7), pp. 759-765; 
Nelson, D., Cox, M., Amino acids peptides and proteins (2005) Lehnninger Principles of Biochemistry, pp. 78-79. , Freeman WH editor. New York: WH Freemand and Company; 
Berger, J., Reist, M., Mayer, J.M., Felt, O., Gurny, R., Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications (2004) European Journal of Pharmaceutics and Biopharmaceutics, 57 (1), pp. 35-52. , DOI 10.1016/S0939-6411(03)00160-7; 
Yamaguchi, F., Ogawa, Y., Kikuchi, M., Yuasa, K., Motai, H., Detection of γ-Polyglutamic acid (γ-PGA) by SDS-PAGE (1996) Biosci Biotech Biochem, 60 (2), pp. 255-258; 
Antunes, J.C., Pereira, C.L., Molinos, M., Ferreira-Da-Silva, F., Gon{\c c}alves, R.M., Barbosa, M.A., Layer-by-layer self-assembly of polyelectrolyte complexes of Chitosam and poly(gamma-glutamic acid) (2011) Biomacromolecules, 12 (12), pp. 4183-4195; 
Georgiou, C., Grintzalis, K., Zervoudakis, G., Papapostolou, I., Mechanism of Coomassie brilliant blue G-250 binding to proteins: A hydrophobic assay for nanogram quantities of proteins (2008) Anal Bioanal Chem, 391 (1), pp. 391-403; 
Socrates, G., (2001) Infrared and Raman Characteristic Group Frequencies - Table and Charts, , England: Jonh Wiley \&Sons Ltd; 
Birrer, G.A., Cromwick, A.M., Gross, R.A., Gamma-poly(glutamic acid) formation by Bacillus licheniformis 9945a: Physiological and biochemical studies (1994) Int J Biol Macromol, 16 (5), pp. 265-275; 
Calvo, P., Remunan-Lopez, C., Vila-Jato, J.L., Alonso, M.J., Novel hydrophilic chitosan-polyethylene oxide nanoparticles as protein carriers (1997) Journal of Applied Polymer Science, 63 (1), pp. 125-132
},
	month = {2012///},
	pages = {1583 - 1591},
	abstract = {The remarkable properties of poly-aminoacids, mainly their biocompatibility and biodegradability, have prompted an increasing interest in these polymers for biomedical applications. Poly-γ-glutamic acid (γ-PGA) is one of the most interesting poly-aminoacids with potential applications as a biomaterial. Here we describe the production and characterization of γ-PGA by Bacillus subtilis natto. The γ-PGA was produced with low molecular weight (10-50 kDa), high purity grade (>99 \%) and a D-/L-glutamate ratio of 50-60/50-40 \%. To evaluate the feasibility of using this γ-PGA as a biomaterial, chitosan (Ch)/γ-PGA nanoparticles were prepared by the coacervation method at pH ranging from 3.0 to 5.0, with dimensions in the interval 214-221 nm with a poly-dispersion index of ca. 0.2. The high purity of γ-PGA produced by this method, which is firstly described here, renders this biopolymer suitable for biomedical applications. Moreover, the Ch/γ-PGA nanocomplexes developed in this investigation can be combined with biologically active substances for their delivery in the organism. The fact that the assembly between Ch and γ-PGA relies on electrostatic interactions enables addition of other molecules that can be released into the medium through changes from acidic to physiological pH, without loss in biological activity. {\textcopyright} Springer Science+Business Media, LLC 2012.},
	keywords = {Amino acids, animal cell, article, Bacillus subtilis, Bacillus subtilis natto, Bioactivity, Biocompatibility, Biocompatible Materials, biodegradability, Biodegradable polymers, Biodegradation, Biologically active substances, biomaterial, Biomedical applications, biomedicine, biosynthesis, chitosan, circular dichroism, complex formation, controlled study, dispersion, Electrophoresis, Polyacrylamide Gel, feasibility study, Functional polymers, gamma polyglutamic acid, Glutamic acid, High purity, Low molecular weight, Medical applications, molecular interaction, molecular weight, Nanocomplexes, nanoparticle, nonhuman, pH, Physiological pH, polyglutamic acid, Potential applications, priority journal, purification, Spectroscopy, Fourier Transform Infrared, static electricity, Stereoisomerism, unclassified drug},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84863314791\&partnerID=40\&md5=d0d618bb2684c8e80e984cfe35f3eb77},
	author = {Pereira, C.L. and Antunes, J.C. and Gon{\c c}alves, R.M. and Ferreira-da-Silva, F. and Barbosa, M.A.}
}
@article {2180,
	title = {Chitosan drives anti-inflammatory macrophage polarisation and pro-inflammatory dendritic cell stimulation.},
	journal = {European cells \& materialsEur Cell Mater},
	volume = {24},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusPubMed ID: 22828991Language of Original Document: EnglishCorrespondence Address: Oliveira, M.I.Chemicals/CAS: chitosan, 9012-76-4; Antigens, CD86; Biocompatible Materials; Chitosan, 9012-76-4; Histocompatibility Antigens Class II; IL10 protein, human; Interleukin-10, 130068-27-8; Transforming Growth Factor beta1; Tumor Necrosis Factor-alpha},
	month = {2012///},
	pages = {136 - 152; discussion 152-153},
	abstract = {Macrophages and dendritic cells (DC) share the same precursor and play key roles in immunity. Modulation of their behaviour to achieve an optimal host response towards an implanted device is still a challenge. Here we compare the differentiation process and polarisation of these related cell populations and show that they exhibit different responses to chitosan (Ch), with human monocyte-derived macrophages polarising towards an anti-inflammatory phenotype while their DC counterparts display pro-inflammatory features. Macrophages and DC, whose interactions with biomaterials are frequently analysed using fully differentiated cells, were cultured directly on Ch films, rather than exposed to the polymer after complete differentiation. Ch was the sole stimulating factor and activated both macrophages and DC, without leading to significant T cell proliferation. After 10 d on Ch, macrophages significantly down-regulated expression of pro-inflammatory markers, CD86 and MHCII. Production of pro-inflammatory cytokines, particularly TNF-α, decreased with time for cells cultured on Ch, while anti-inflammatory IL-10 and TGF-β1, significantly increased. Altogether, these results suggest an M2c polarisation. Also, macrophage matrix metalloproteinase activity was augmented and cell motility was stimulated by Ch. Conversely, DC significantly enhanced CD86 expression, reduced IL-10 secretion and increased TNF-α and IL-1β levels. Our findings indicate that cells with a common precursor may display different responses, when challenged by the same biomaterial. Moreover, they help to further comprehend macrophage/DC interactions with Ch and the balance between pro- and anti-inflammatory signals associated with implant biomaterials. We propose that an overall pro-inflammatory reaction may hide the expression of anti-inflammatory cytokines, likely relevant for tissue repair/regeneration.},
	keywords = {antibody specificity, Antigens, CD86, article, Biocompatible Materials, biomaterial, biosynthesis, CD86 antigen, Cell culture, cell differentiation, cell motion, Cell Movement, Cells, Cultured, chitosan, cytology, dendritic cell, Dendritic Cells, drug effect, flow cytometry, Histocompatibility Antigens Class II, HLA antigen class 2, human, Humans, IL10 protein, human, immunology, interleukin 10, Interleukin-10, macrophage, Macrophages, metabolism, monocyte, Monocytes, Organ Specificity, transforming growth factor beta1, tumor necrosis factor alpha, Tumor Necrosis Factor-alpha},
	isbn = {14732262 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84868253478\&partnerID=40\&md5=5a496411709ccbe5d3d70de13b58b08a},
	author = {Oliveira, M.I. and Santos, S.G. and Oliveira, M.J. and Torres, A.L. and Barbosa, M.A.}
}
@article {2187,
	title = {The effect of adsorbed fibronectin and osteopontin on macrophage adhesion and morphology on hydrophilic and hydrophobic model surfaces},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {8},
	year = {2012},
	note = {Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2012.06.010Language of Original Document: EnglishCorrespondence Address: Barbosa, M.A.; INEB - Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: mbarbosa@ineb.up.ptChemicals/CAS: fibronectin, 86088-83-7; gold, 7440-57-5; osteopontin, 106441-73-0References: Anderson, J.M., Biological responses to materials (2001) Annu Rev Mater Res, 31, pp. 81-110;Horbett, T.A., Brash, J.L., Proteins at interfaces: Current issues and future prospects (1987) Proteins at Interfaces, pp. 1-33; 
Dee, K.C., Puleo, D.A., Bizios, R., Protein-surface interactions (2003) An Introduction to Tissue-biomaterial Interactions, pp. 37-37. , New York: John Wiley \& Sons Inc; 
Wilson, C.J., Clegg, R.E., Leavesley, D.I., Pearcy, M.J., Mediation of biomaterial-cell interactions by adsorbed proteins: A review (2005) Tissue Eng, 11, pp. 1-18; 
Allen, L.T., Surface-induced changes in protein adsorption and implications for cellular phenotypic responses to surface interaction (2006) Biomaterials, 27, pp. 3096-3108; 
Kou, P.M., Babensee, J.E., Macrophage and dendritic cell phenotypic diversity in the context of biomaterials (2011) J Biomed Mater Res, 96, pp. 239-260; 
Defife, K.M., Colton, E., Nakayama, Y., Matsuda, T., Anderson, J.M., Spatial regulation and surface chemistry control of monocyte/macrophage adhesion and foreign body giant cell formation by photochemically micropatterned surfaces (1999) J Biomed Mater Res, 45, pp. 148-154; 
Collier, T.O., Anderson, J.M., Kikuchi, A., Okano, T., Adhesion behavior of monocytes, macrophages, and foreign body giant cells on poly(n-isopropylacrylamide) temperature-responsive surfaces (2002) J Biomed Mater Res, 59, pp. 136-143; 
Jenney, C.R., Anderson, J.M., Effects of surface-coupled polyethylene oxide on human macrophage adhesion and foreign body giant cell formation in vitro (1999) J Biomed Mater Res, 44, pp. 206-216; 
Jenney, C.R., Anderson, J.M., Alkylsilane-modified surfaces: Inhibition of human macrophage adhesion and foreign body giant cell formation (1999) J Biomed Mater Res, 46, pp. 11-21; 
Collier, T.O., Anderson, J.M., Protein and surface effects on monocyte and macrophage adhesion, maturation, and survival (2002) J Biomed Mater Res, 60, pp. 487-496; 
Kao, W.J., Hubbell, J.A., Anderson, J.M., Protein-mediated macrophage adhesion and activation on biomaterials: A model for modulating cell behavior (1999) J Mater Sci Mater Med, 10, pp. 601-605; 
Jenney, C.R., Anderson, J.M., Adsorbed serum proteins responsible for surface dependent human macrophage behavior (2000) J Biomed Mater Res, 49, pp. 435-447; 
Jenney, C.R., Anderson, J.M., Adsorbed IgG: A potent adhesive substrate for human macrophages (2000) J Biomed Mater Res, 50, pp. 281-290; 
Kao, W.J., Lee, D., Schense, J.C., Hubbell, J.A., Fibronectin modulates macrophage adhesion and FBGC formation: The role of RGD, PHSRN, and PRRARV domains (2001) J Biomed Mater Res, 55, pp. 79-88; 
Kao, W.J., Evaluation of protein-modulated macrophage behavior on biomaterials: Designing biomimetic materials for cellular engineering (1999) Biomaterials, 20, pp. 2213-2221; 
Giamblanco, N., Yaseen, M., Zhavnerko, G., Lu, J.R., Marletta, G., Fibronectin conformation switch induced by coadsorption with human serum albumin (2010) Langmuir, 27, pp. 312-319; 
Anderson, J.M., The extracellular matrix and biomaterials (1994) Implantation Biology: The Host Response and Biomedical Devices, pp. 113-130; 
Pankov, R., Yamada, K.M., Fibronectin at a glance (2002) J Cell Sci, 115, pp. 3861-3863; 
Pierschbacher, M.D., Ruoslahti, E., Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule (1984) Nature, 309, pp. 30-33; 
Lund, S., Giachelli, C., Scatena, M., The role of osteopontin in inflammatory processes (2009) J Cell Commun Signal, 3, pp. 311-322; 
Denhardt, D.T., Giachelli, C.M., Rittling, S.R., Role of osteopontin in cellular signaling and toxicant injury (2001) Annu Rev Pharmacol Toxicol, 41, pp. 723-749; 
Denhardt, D.T., Noda, M., O{\textquoteright}Regan, A.W., Pavlin, D., Berman, J.S., Osteopontin as a means to cope with environmental insults: Regulation of inflammation, tissue remodeling, and cell survival (2001) J Clin Invest, 107, pp. 1055-1061; 
Denhardt, D., Guo, X., Osteopontin: A protein with diverse functions (1993) FASEB J, 7, pp. 1475-1482; 
Kazanecki, C.C., Uzwiak, D.J., Denhardt, D.T., Control of osteopontin signaling and function by post-translational phosphorylation and protein folding (2007) J Cell Biochem, 102, pp. 912-924; 
Pedraza, C.E., Nikolcheva, L.G., Kaartinen, M.T., Barralet, J.E., McKee, M.D., Osteopontin functions as an opsonin and facilitates phagocytosis by macrophages of hydroxyapatite-coated microspheres: Implications for bone wound healing (2008) Bone, 43, pp. 708-716; 
Giachelli, C.M., Lombardi, D., Johnson, R.J., Murry, C.E., Almeida, M., Evidence for a role of osteopontin in macrophage infiltration in response to pathological stimuli in vivo (1998) Am J Pathol, 152, pp. 353-358; 
Tsai, A.T., Rice, J., Scatena, M., Liaw, L., Ratner, B.D., Giachelli, C.M., The role of osteopontin in foreign body giant cell formation (2005) Biomaterials, 26, pp. 5835-5843; 
Jensen, T., Osteopontin functionalization of hydroxyapatite nanoparticles in a PDLLA matrix promotes bone formation (2011) J Biomed Mater Res, 99, pp. 94-101; 
Mrksich, M., Whitesides, G.M., Using self-assembled monolayers to understand the interactions of man-made surfaces with proteins and cells (1996) Annu Rev Biophys Biomol, 25, pp. 55-78; 
Barrias, C.C., Martins, M.C.L., Almeida-Porada, G., Barbosa, M.A., Granja, P.L., The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers (2009) Biomaterials, 30, pp. 307-316; 
Martins, M.C., Fonseca, C., Barbosa, M.A., Ratner, B.D., Albumin adsorption on alkanethiols self-assembled monolayers on gold electrodes studied by chronopotentiometry (2003) Biomaterials, 24, pp. 3697-3706; 
Sivaraman, B., Fears, K.P., Latour, R.A., Investigation of the effects of surface chemistry and solution concentration on the conformation of adsorbed proteins using an improved circular dichroism method (2009) Langmuir, 25, pp. 3050-3056; 
MacIel, J., Martins, M.C.L., Barbosa, M.A., The stability of self-assembled monolayers with time and under biological conditions (2010) J Biomed Mater Res, 94, pp. 833-843; 
Martins, M.C., Ratner, B.D., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methyl-terminated alkanethiols self-assembled monolayers (2003) J Biomed Mater Res, 67, pp. 158-171; 
Gehanno, V., Freitas, P.P., Veloso, A., Ferrira, J., Almeida, B., Soasa, J.B., Ion beam deposition of Mn-Ir spin valves (1999) IEEE Trans Magn, 35, pp. 4361-4367; 
Martins, M.C.L., Curtin, S.A., Freitas, S.C., Salgueiro, P., Ratner, B.D., Barbosa, M.A., Molecularly designed surfaces for blood deheparinization using an immobilized heparin-binding peptide (2009) J Biomed Mater Res, 88, pp. 162-173; 
McNally, A.K., Anderson, J.M., Complement C3 participation in monocyte adhesion to different surfaces (1994) Proc Natl Acad Sci USA, 91, pp. 10119-10123; 
Martins, M.C., Sousa, S., Antunes, J., Barbosa, M., Protein adsorption characterization (2012) Nanotechnology in Regenerative Medicine, pp. 141-161; 
Barrias, C.C., Martins, C.L., Miranda, C.S., Barbosa, M.A., Adsorption of a therapeutic enzyme to self-assembled monolayers: Effect of surface chemistry and solution pH on the amount and activity of adsorbed enzyme (2005) Biomaterials, 26, pp. 2695-2704; 
Goncalves, R., Martins, M.C.L., Oliveira, M.J., Almeida-Porada, G., Barbosa, M.A., Bioactivity of immobilized EGF on self-assembled monolayers: Optimization of the immobilization process (2010) J Biomed Mater Res, 94, pp. 576-585; 
(1993) Iodine-125. A Guide to Radioiodination Techniques, , Amersham Life Science Little Chalfont, UK: Amersham, International; 
Goncalves, I.C., Martins, M.C., Barbosa, M.A., Naeemi, E., Ratner, B.D., Selective protein adsorption modulates platelet adhesion and activation to oligo(ethylene glycol)-terminated self-assembled monolayers with C18 ligands (2009) J Biomed Mater Res, 89, pp. 642-653; 
Burmeister, J.S., Vrany, J.D., Reichert, W.M., Truskey, G.A., Effect of fibronectin amount and conformation on the strength of endothelial cell adhesion to HEMA/EMA copolymers (1996) J Biomed Mater Res, 30, pp. 13-22; 
Shen, M., Horbett, T.A., The effects of surface chemistry and adsorbed proteins on monocyte/macrophage adhesion to chemically modified polystyrene surfaces (2001) J Biomed Mater Res, 57, pp. 336-345; 
Brodbeck, W.G., Colton, E., Anderson, J.M., Effects of adsorbed heat labile serum proteins and fibrinogen on adhesion and apoptosis of monocytes/macrophages on biomaterials (2003) J Mater Sci Mater Med, 14, pp. 671-675; 
Curtis, A.S., Forrester, J.V., The competitive effects of serum proteins on cell adhesion (1984) J Cell Sci, 71, pp. 17-35; 
Roach, P., Farrar, D., Perry, C.C., Interpretation of protein adsorption: Surface-induced conformational changes (2005) J Am Chem Soc, 127, pp. 8168-8173; 
Harder, P., Grunze, M., Dahint, R., Whitesides, G.M., Laibinis, P.E., Molecular conformation in oligo(ethylene glycol)-terminated self-assembled monolayers on gold and silver surfaces determines their ability to resist protein adsorption (1998) J Phys Chem B, 102, pp. 426-436; 
Stein, M.J., Weidner, T., McCrea, K., Castner, D.G., Ratner, B.D., Hydration of sulphobetaine and tetra(ethylene glycol)-terminated self-assembled monolayers studied by sum frequency generation vibrational spectroscopy (2009) J Phys Chem B, 113, pp. 11550-11556; 
Latour, R.A., Biomaterials: Protein-surface interactions (2008) The Encyclopedia of Biomaterials and Biomedical Engineering, pp. 270-284; 
Michael, K.E., Vernekar, V.N., Keselowsky, B.G., Meredith, J.C., Latour, R.A., Garc{\'\i}a, A.J., Adsorption-induced conformational changes in fibronectin due to interactions with well-defined surface chemistries (2003) Langmuir, 19, pp. 8033-8040; 
Keselowsky, B.G., Collard, D.M., Garc{\'\i}a, A.J., Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion (2003) J Biomed Mater Res, 66, pp. 247-259; 
Keselowsky, B.G., Collard, D.M., Garc{\'\i}a, A.J.A.J., Surface chemistry modulates focal adhesion composition and signaling through changes in integrin binding (2004) Biomaterials, 25, pp. 5947-5954; 
Wittmer, C.R., Phelps, J.A., Saltzman, W.M., Van Tassel, P.R., Fibronectin terminated multilayer films: Protein adsorption and cell attachment studies (2007) Biomaterials, 28, pp. 851-860; 
McNally, A.K., Anderson, J.M., 1 and β2 integrins mediate adhesion during macrophage fusion and multinucleated foreign body giant cell formation (2002) Am J Pathol, 160, pp. 621-630; 
Liu, L., Chen, S., Giachelli, C.M., Ratner, B.D., Jiang, S., Controlling osteopontin orientation on surfaces to modulate endothelial cell adhesion (2005) J Biomed Mater Res, 74, pp. 23-31; 
Malmstrom, J., Christensen, B., Lovmand, J., Sorensen, E.S., Duch, M., Sutherland, D.S., Osteopontin presentation affects cell adhesion - Influence of underlying surface chemistry and nanopatterning of osteopontin (2010) J Biomed Mater Res, 95, pp. 518-530
},
	month = {2012///},
	pages = {3669 - 3677},
	abstract = {Macrophages play a crucial role in the host response to biomaterials. Here we investigated the effect of adsorbed fibronectin (FN) and osteopontin (OPN), two important proteins for tissue repair, on macrophage adhesion and morphology. Since cell-biomaterial interactions are modulated via proteins adsorbed onto biomaterial surfaces, FN and OPN were adsorbed on model self-assembled monolayers (SAMs) of alkanethiols on gold with different functional terminal groups (CH                        3, OH and tetra(ethylene-glycol)). The initial interaction of inflammatory cells with a biomaterial is crucial for the ensuing phases of an inflammatory reaction. For this reason short-term cultures of primary human macrophages were performed. To account for the competitive adsorption of other proteins serum was added to the culture medium and the effect compared with serum-free medium cultures. In the presence of serum hydrophilic surfaces increased macrophage adhesion. In particular, FN induced a higher cell density, while OPN tended to decrease it. In serum-free medium cell adhesion was greater on hydrophobic surfaces, except for OPN-coated SAMs. Importantly, FN no longer enhanced macrophage adhesion, while OPN maintained its inhibitory effect. Cell polarization studies indicated that macrophage morphology variations induced by surface chemistry are overcome by pre-adsorbed OPN. Taken together our results show that in the presence of serum macrophage adhesion is promoted by FN hydrophilic surfaces, but impaired on OPN-coated surfaces. The effects of inhibited macrophage adhesion on macrophage fusion, and its relevance to the initial stages of the inflammatory response to biomaterials are discussed. {\textcopyright} 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
},
	keywords = {alkane, article, biomaterial, Biomaterials, cell adhesion, cell density, cell interaction, cell structure, controlled study, culture medium, cytoskeleton, Fibronectin, functional group, gold, human, human cell, hydrophilicity, hydrophobicity, inflammatory cell, macrophage, Macrophages, nanomaterial, Osteopontin, priority journal, self assembled monolayer, Self-assembled monolayers, Tetra, thiol, tissue repair},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84865455542\&partnerID=40\&md5=9d34fb780e9ce53b424143b0f3eefb97},
	author = {Maciel, J. and Oliveira, M.I. and Gonalves, R.M. and Barbosa, M.A.}
}
@article {2211,
	title = {Enhanced mesenchymal stromal cell recruitment via natural killer cells by incorporation of inflammatory signals in biomaterials},
	journal = {Journal of the Royal Society InterfaceJ. R. Soc. Interface},
	volume = {9},
	year = {2012},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: Scopusdoi: 10.1098/rsif.2011.0357Language of Original Document: EnglishCorrespondence Address: Almeida, C.R.; INEB-Instituto de Engenharia Biom{\'e}dica, Biomaterials Division, NEWTherapies Group, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: cralmeid@ineb.up.ptChemicals/CAS: alkaline phosphatase, 9001-78-9; chitosan, 9012-76-4; fibrinogen, 9001-32-5; gamma interferon, 82115-62-6References: Mountziaris, P.M., Mikos, A.G., Modulation of the inflammatory response for enhanced bone tissue regeneration (2008) Tissue Eng. Part B Rev., 14, pp. 179-186. , doi:10. 1089/ten.teb.2008.0038;Anderson, J.M., Rodriguez, A., Chang, D.T., Foreign body reaction to biomaterials (2008) Semin. Immunol., 20, pp. 86-100. , doi:10.1016/j.smim.2007.11.004; 
Agaiby, A.D., Dyson, M., Immuno-inflammatory cell dynamics during cutaneous wound healing (1999) J. Anat., 195, pp. 531-542. , doi:10.1046/j.1469-7580.1999.19540531.x; 
Vivier, E., Tomasello, E., Baratin, M., Walzer, T., Ugolini, S., Functions of natural killer cells (2008) Nat. Immunol., 9, pp. 503-510. , doi:10.1038/ni1582; 
Aggarwal, S., Pittenger, M.F., Human mesenchymal stem cells modulate allogeneic immune cell responses (2005) Blood, 105, pp. 1815-1822. , doi:10.1182/blood- 2004-04-1559; 
Caplan, A.I., Adult mesenchymal stem cells for tissue engineering versus regenerative medicine (2007) J. Cell. Physiol., 213, pp. 341-347. , doi:10.1002/jcp.21200; 
Pittenger, M.F., Multilineage potential of adult human mesenchymal stem cells (1999) Science, 284, pp. 143-147. , doi:10.1126/science.284.5411.143; 
Poggi, A., Prevosto, C., Massaro, A.M., Negrini, S., Urbani, S., Pierri, I., Saccardi, R., Zocchi, M.R., Interaction between human NK cells and bone marrow stromal cells induces NK cell triggering: Role of NKp30 and NKG2D receptors (2005) J. Immunol., 175, pp. 6352-6360. , doi:10.175/10/6352[pii]; 
Rasmusson, I., Ringden, O., Sundberg, B., Le Blanc, K., Mesenchymal stem cells inhibit the formation of cytotoxic T lymphocytes, but not activated cytotoxic T lymphocytes or natural killer cells (2003) Transplantation, 76, pp. 1208-1213. , doi:10.1097/01.TP.0000082540.43730.80; 
G{\"o}therstr{\"o}m, C., Lundqvist, A., Duprez, I.R., Childs, R., Berg, L., Le Blanc, K., Fetal and adult multipotent mesenchymal stromal cells are killed by different pathways (2010) Cytotherapy, 13, pp. 269-278. , doi:10.3109/14653249.2010.523077; 
Jewett, A., Arasteh, A., Tseng, H.C., Behel, A., Arasteh, H., Yang, W., Cacalano, N.A., Paranjpe, A., Strategies to rescue mesenchymal stem cells (MSCs) and dental pulp stem cells (DPSCs) from NK cell mediated cytotoxicity (2010) PLoS ONE, 5, pp. e9874. , doi:10.1371/journal.pone. 0009874; 
Sotiropoulou, P.A., Perez, S.A., Gritzapis, A.D., Baxevanis, C.N., Papamichail, M., Interactions between human mesenchymal stem cells and natural killer cells (2006) Stem Cells, 24, pp. 74-85. , doi:10.1634/stemcells.2004-0359; 
Spaggiari, G.M., Capobianco, A., Becchetti, S., Mingari, M.C., Moretta, L., Mesenchymal stem cell-natural killer cell interactions: Evidence that activated NK cells are capable of killing MSCs, whereas MSCs can inhibit IL-2- Induced NK-cell proliferation (2006) Blood, 107, pp. 1484-1490. , doi:10.1182/blood-2005-07-2775; 
Tseng, H.C., Increased lysis of stem cells but not their differentiated cells by natural killer cells; dedifferentiation or reprogramming activates NK cells (2010) PLoS ONE, 5, pp. e11590. , doi:10.1371/journal.pone.0011590; 
Krampera, M., Role for interferon-gamma in the immunomodulatory activity of human bone marrow mesenchymal stem cells (2006) Stem Cells, 24, pp. 386-398. , doi:10.1634/stemcells.2005-0008; 
Pradier, A., Passweg, J., Villard, J., Kindler, V., Human bone marrow stromal cells and skin fibroblasts inhibit natural killer cell proliferation and cytotoxic activity (2010) Cell Transplant., , doi:10.3727/096368910X 536545; 
Selmani, Z., Human leukocyte antigen-G5 secretion by human mesenchymal stem cells is required to suppress T lymphocyte and natural killer function and to induce CD4+CD25highFOXP3+ regulatory T cells (2008) Stem Cells, 26, pp. 212-222. , doi:10.1634/stemcells. 2007-0554; 
Spaggiari, G.M., Capobianco, A., Abdelrazik, H., Becchetti, F., Mingari, M.C., Moretta, L., Mesenchymal stem cells inhibit natural killer-cell proliferation, cytotoxicity, and cytokine production: Role of indoleamine 2,3-dioxygenase and prostaglandin E2 (2008) Blood, 111, pp. 1327-1333. , doi:10.1182/blood-2007-02- 074997; 
Salem, H.K., Thiemermann, C., Mesenchymal stromal cells: Current understanding and clinical status (2010) Stem Cells, 28, pp. 585-596. , doi:10.1002/stem.269; 
Dimitriou, R., Tsiridis, E., Giannoudis, P.V., Current concepts of molecular aspects of bone healing (2005) Injury, 36, pp. 1392-1404. , doi:10.1016/j.injury.2005.07.019; 
Rifas, L., T-cell cytokine induction of BMP-2 regulates human mesenchymal stromal cell differentiation and mineralization (2006) J. Cell. Biochem., 98, pp. 706-714. , doi:10.1002/jcb.20933; 
Rifas, L., Arackal, S., Weitzmann, M.N., Inflammatory T cells rapidly induce differentiation of human bone marrow stromal cells into mature osteoblasts (2003) J. Cell. Biochem., 88, pp. 650-659. , doi:10.1002/jcb.10436; 
Champagne, C.M., Takebe, J., Offenbacher, S., Cooper, L.F., Macrophage cell lines produce osteoinductive signals that include bone morphogenetic protein-2 (2002) Bone, 30, pp. 26-31. , doi:10.1016/S8756-3282(01)00638-X; 
Gerstenfeld, L.C., Cho, T.J., Kon, T., Aizawa, T., Cruceta, J., Graves, B.D., Einhorn, T.A., Impaired intramembranous bone formation during bone repair in the absence of tumor necrosis factor-alpha signaling (2001) Cells Tissues Organs, 169, pp. 285-294. , doi:10.1159/ 000047893; 
Gerstenfeld, L.C., Cho, T.J., Kon, T., Aizawa, T., Tsay, A., Fitch, J., Barnes, G.L., Einhorn, T.A., Impaired fracture healing in the absence of TNF-alpha signaling: The role of TNF-α in endochondral cartilage resorption (2003) J. Bone Miner. Res., 18, pp. 1584-1592. , doi:10.1359/jbmr.2003.18.9.1584; 
Duque, G., Huang, D.C., Macoritto, M., Rivas, D., Yang, X.F., Ste-Marie, L.G., Kremer, R., Autocrine regulation of interferon gamma in mesenchymal stem cells plays a role in early osteoblastogenesis (2009) Stem Cells, 27, pp. 550-558. , doi:10.1634/stemcells.2008-0886; 
Honczarenko, M., Le, Y., Swierkowski, M., Ghiran, I., Glodek, A.M., Silberstein, L.E., Human bone marrow stromal cells express a distinct set of biologically functional chemokine receptors (2006) Stem Cells, 24, pp. 1030-1041. , doi:10.1634/stemcells.2005-0319; 
Robertson, M.J., Role of chemokines in the biology of natural killer cells (2002) J. Leukoc Biol., 71, pp. 173-183; 
Rodrigues, S.N., Gon{\c c}alves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers (2006) Biomaterials, 27, pp. 5357-5367. , doi:10.1016/j.biomaterials.2006.06.010; 
Peled, E., Boss, J., Bejar, J., Zinman, C., Seliktar, D., A novel poly(ethylene glycol)-fibrinogen hydrogel for tibial segmental defect repair in a rat model (2007) J. Biomed. Mater. Res., 80, pp. 874-884. , doi:10.1002/jbm. a.30928; 
Bryceson, Y.T., March, M.E., Ljunggren, H.G., Long, E.O., Activation, coactivation, and costimulation of resting human natural killer cells (2006) Immunol. Rev., 214, pp. 73-91. , doi:10.1111/j.1600-065X.2006.00457.x; 
Muto, S., Vetvicka, V., Ross, G.D., CR3 (CD11b/ CD18) expressed by cytotoxic T cells and natural killer cells is upregulated in a manner similar to neutrophil CR3 following stimulation with various activating agents (1993) J. Clin. Immunol., 13, pp. 175-184. , doi:10.1007/ BF00919970; 
Ross, G.D., Vetvicka, V., CR3 (CD11b, CD18): A phagocyte and NK cell membrane receptor with multiple ligand specificities and functions (1993) Clin. Exp. Immunol., 92, pp. 181-184. , doi:10.1111/j.1365-2249.1993.tb03377.x; 
Lishko, V.K., Kudryk, B., Yakubenko, V.P., Yee, V.C., Ugarova, T.P., Regulated unmasking of the cryptic binding site for integrin alpha M beta 2 in the gamma C-domain of fibrinogen (2002) Biochemistry, 41, pp. 12942-12951. , doi:10.1021/bi026324c; 
Mwale, F., Iordanova, M., Demers, C.N., Steffen, T., Roughley, P., Antoniou, J., Biological evaluation of chitosan salts cross-linked to genipin as a cell scaffold for disk tissue engineering (2005) Tissue Eng., 11, pp. 130-140. , doi:10.1089/ten.2005.11.130; 
Prabaharan, M., Jayakumar, R., Chitosan-graft-beta-cyclodextrin scaffolds with controlled drug release capability for tissue engineering applications (2009) Int. J. Biol. Macromol., 44, pp. 320-325. , doi:10.1016/j.ijbiomac.2009.01.005; 
Agnihotri, S.A., Mallikarjuna, N.N., Aminabhavi, T.M., Recent advances on chitosan-based micro-and nanoparticles in drug delivery (2004) J. Control Release, 100, pp. 5-28. , doi:10.1016/j.jconrel.2004.08.010; 
Barbosa, M.A., Granja, P.L., Barrias, C.C., Amaral, I.F., Polysaccharides as scaffolds for bone regeneration (2005) ITBM-RBM, 26, pp. 212-217. , doi:10.1016/j.rbmret.2005.04.006; 
Berger, J., Reist, M., Mayer, J.M., Felt, O., Gurny, R., Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications (2004) Eur. J. Pharm. Biopharm., 57, pp. 35-52. , doi:10.1016/S0939-6411(03)00160-7; 
Amaral, I.F., Cordeiro, A.L., Sampaio, P., Barbosa, M.A., Attachment, spreading and short-term proliferation of human osteoblastic cells cultured on chitosan films with different degrees of acetylation (2007) J. Biomater. Sci. Polym. Ed., 18, pp. 469-485. , doi:10.1163/ 156856207780425068; 
Barbosa, J.N., Amaral, I.F., Aguas, A.P., Barbosa, M.A., Evaluation of the effect of the degree of acetylation on the inflammatory response to 3D porous chitosan scaffolds (2010) J. Biomed. Mater. Res., 93 A, pp. 20-28. , doi:10.1002/jbm.a.32499; 
Oliveira, S.M., Mijares, D.Q., Turner, G., Amaral, I.F., Barbosa, M.A., Teixeira, C.C., Engineering endochondral bone: In vivo studies (2009) Tissue Eng. Part A, 15, pp. 635-643. , doi:10.1089/ten.tea.2008.0052; 
Amaral, I.F., Sampaio, P., Barbosa, M.A., Three-dimensional culture of human osteoblastic cells in chitosan sponges: The effect of the degree of acetylation (2006) J. Biomed. Mater. Res., 76, pp. 335-346. , doi:10.1002/jbm.a.30522; 
Goncalves, R.M., Martins, M.C., Almeida-Porada, G., Barbosa, M.A., Induction of notch signaling by immobilization of jagged-1 on self-assembled monolayers (2009) Biomaterials, 30, pp. 6879-6887. , doi:10.1016/j.biomaterials.2009.09.010; 
Dominici, M., Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement (2006) Cytotherapy, 8, pp. 315-317. , doi:10.1080/14653240600855905; 
Siddappa, R., Licht, R., Van Blitterswijk, C., De Boer, J., Donor variation and loss of multipotency during in vitro expansion of human mesenchymal stem cells for bone tissue engineering (2007) J. Orthop. Res., 25, pp. 1029-1041. , doi:10.1002/jor.20402; 
Maeda, Y., Kimura, Y., Antitumor Effects of Various Low-Molecular-Weight Chitosans Are Due to Increased Natural Killer Activity of Intestinal Intraepithelial Lymphocytes in Sarcoma 180-Bearing Mice (2004) Journal of Nutrition, 134 (4), pp. 945-950; 
Zaharoff, D.A., Rogers, C.J., Hance, K.W., Schlom, J., Greiner, J.W., Chitosan solution enhances both humoral and cell-mediated immune responses to subcutaneous vaccination (2007) Vaccine, 25, pp. 2085-2094. , doi:10. 1016/j.vaccine.2006.11.034; 
Tang, L., Eaton, J.W., Fibrin(ogen) mediates acute inflammatory responses to biomaterials (1993) J. Exp. Med., 178, pp. 2147-2156. , doi:10.1084/jem.178.6.2147; 
Drew, A.F., Liu, H., Davidson, J.M., Daugherty, C.C., Degen, J.L., Wound-healing defects in mice lacking fibrinogen (2001) Blood, 97, pp. 3691-3698. , doi:10.1182/blood. V97.12.3691; 
Flick, M.J., Du, X., Degen, J.L., Fibrin(ogen)- alpha M beta 2 interactions regulate leukocyte function and innate immunity in vivo (2004) Exp. Biol. Med. (Maywood), 229, pp. 1105-1110. , doi:10.229/11/1105[pii]; 
Culley, F.J., Natural killer cell signal integration balances synapse symmetry and migration (2009) PLoS Biol., 7, pp. e1000159. , doi:10.1371/journal.pbio.1000159; 
Dustin, M.L., Cell adhesion molecules and actin cytoskeleton at immune synapses and kinapses (2007) Curr. Opin. Cell Biol., 19, pp. 529-533. , doi:10.1016/j.ceb.2007.08.003; 
Jacobs, R., Hintzen, G., Kemper, A., Beul, K., Kempf, S., Behrens, G., Sykora, K.W., Schmidt, R.E., CD56bright cells differ in their KIR repertoire and cytotoxic features from CD56dim NK cells (2001) Eur. J. Immunol., 31, pp. 3121-3127. , doi:10.1002/1521-4141(2001010)31: 10〈3121::AID-IMMU3121〉3.0. CO;2-4; 
Anfossi, N., Human NK cell education by inhibitory receptors for MHC class I (2006) Immunity, 25, pp. 331-342. , doi:10.1016/j.immuni.2006.06.013; 
Nolte-{\textquoteright}t Hoen, E.N.M., Almeida, C.R., Cohen, N.R., Nedvetzki, S., Yarwood, H., Davis, D.M., Increased surveillance of cells in mitosis by human NK cells suggests a novel strategy for limiting tumor growth and viral replication (2006) Blood, 109, pp. 670-673. , doi:10.1182/blood-2006-07- 036509; 
Hemeda, H., Jakob, M., Ludwig, A.K., Giebel, B., Lang, S., Brandau, S., Interferon-gamma and tumor necrosis factor-alpha differentially affect cytokine expression and migration properties of mesenchymal stem cells (2010) Stem Cells Dev., 19, pp. 693-706; 
Liippo, J., Toriseva, M., K{\"a}h{\"a}ri, V.-M., Natural killer cell in wound healing (2009) Natural Killer Cells - Basic Science and Clinical Application, pp. 519-525. , eds M. Lotze \& A. Thomson, New York, NY: Academic Press; 
Soderstrom, K., Stein, E., Colmenero, P., Purath, U., Muller-Ladner, U., De Matos, C.T., Tarner, I.H., Engleman, E.G., Natural killer cells trigger osteoclastogenesis and bone destruction in arthritis (2010) Proc. Natl Acad. Sci. USA, 107, pp. 13028-13033. , doi:10.1073/pnas.1000546107
},
	month = {2012///},
	pages = {261 - 271},
	abstract = {An exacerbated inflammatory response questions biomaterial biocompatibility, but on the other hand, inflammation has a central role in the regulation of tissue regeneration. Therefore, it may be argued that an {\textquoteright}ideal{\textquoteright} inflammatory response is crucial to achieve efficient tissue repair/regeneration. Natural killer (NK) cells, being one of the first populations arriving at an injury site, can have an important role in regulating bone repair/regeneration, particularly through interactions with mesenchymal stem/stromal cells (MSCs). Here, we studied how biomaterials designed to incorporate inflammatory signals affected NK cell behaviour and NK cell-MSC interactions. Adsorption of the pro-inflammatory molecule fibrinogen (Fg) to chitosan films led to a 1.5-fold increase in adhesion of peripheral blood human NK cells, without an increase in cytokine secretion. Most importantly, it was found that NK cells are capable of stimulating a threefold increase in human bone marrow MSC invasion, a key event taking place in tissue repair, but did not affect the expression of the differentiation marker alkaline phosphatase (ALP). Of significant importance, this NK cell-mediated MSC recruitment was modulated by Fg adsorption. Designing novel biomaterials leading to rational modulation of the inflammatory response is proposed as an alternative to current bone regeneration strategies. {\textcopyright} 2011 The Royal Society.},
	keywords = {Adhesion, Adsorption, alkaline phosphatase, article, Biocompatibility, biomaterial, Biomaterials, blood cell, Bone, bone marrow cell, Bone Regeneration, Bone repair, cell adhesion, Cell culture, cell differentiation, cell function, cell interaction, cell invasion, Cells, chitosan, Chitosan film, cytokine, Cytokine secretions, Differentiation markers, fibrinogen, gamma interferon, human, Human bone marrow, human cell, Inflammation, Inflammatory response, Mesenchymal Stem Cells, mesenchymal stroma cell, Mesenchymal stromal cells, natural killer cell, Natural killer cells, NK cells, ossification, Pathology, Peripheral blood, Phosphatases, protein expression, Tissue, Tissue regeneration, tissue repair},
	isbn = {17425689 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84856583891\&partnerID=40\&md5=8a6b58ebb9ee4c20099877008acde805},
	author = {Almeida, C.R. and Vasconcelos, D.P. and Gon{\c c}alves, R.M. and Barbosa, M.A.}
}
@article {2214,
	title = {Mesenchymal stem cell recruitment by stromal derived factor-1-delivery systems based on chitosan/poly(γ-glutamic acid) polyelectrolyte complexes},
	journal = {European Cells and MaterialsEur. Cells and Mater.},
	volume = {23},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Gon{\c c}alves, R. M.; INEB, R. Campo Alegre, 823, Porto 4150-180, Portugal; email: raquelg@ineb.up.ptChemicals/CAS: chitosan, 9012-76-4; polyglutamic acid, 24991-23-9, 25513-46-6; Biocompatible Materials; CXCL12 protein, human; Chemokine CXCL12; Chitosan, 9012-76-4; Membranes, Artificial; Polyglutamic Acid, 25513-46-6; poly(gamma-glutamic acid)References: Almeida, C.R., Vasconcelos, D.P., Gon{\c c}alves, R., Barbosa, M.A., Enhanced me s enchymal stromal cell recruitment via Natural Killer cells by incorporation of inflammatory signals in biomaterials (2012) J R Soc Interface, 9, pp. 261-271;Amersham, Iodine-125. A guide to radioiodination techniques (1993) Amersham Life Science, , (I.-A.g.t.r. Techniques, Ed). Amersham, Little Chalfort, UK; 
Antunes, J., Pereira, C.L., Molinos, M., da Ferreira, S.F., Dessi, M., Gloria, A., Ambrosio, L., Barbosa, M.A., Layer-by-layer self-assembly of Chitosan and Poly(γ-glutamic acid) into polyelectrolyte complexes (2011) Biomacromolecules, 12, pp. 4183-4195; 
Barbosa, M., P{\^e}go, A.P., Amaral, I.F., (2011) Chitosan. In: Comprehensive Biomaterials, , 1 st ed (Hutmacher DW, Grainger DW, Healy K, Kirkpatrick CJ, Ducheyne P, eds), Elsevier Amsterdam; 
Bhakta, S., Hong, P., Koc, O., The surface adhesion molecule CXCR4 stimulat es mesenchymal stem cell migration to stromal cell-derived factor-1 in vitro but does not decrease apoptosis under serum deprivation (2006) Cardiovasc Revasc Med, 7, pp. 19-24; 
Bleul, C., Fuhlbrigge, R.C., Casasnovas, J.M., Aiuti, A., Springer, T.A., A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1) (1996) J Exp Med, 184, pp. 110l-1109; 
Boudou, T., Crouzier, T., Ren, K., Blin, G., Picart, C., Multiple functionalities of polyelectrolyte multilayer films: New biomedical applications (2010) Adv Mat, 22, pp. 441-467; 
Broxmeyer, H.E., Christopherson, K.W., Stromal cell derived factor-1/CXCL12, CXCR4 and CD26 in the mobilization and homing of hematopoietic stem and progenitor cells (2004) Curr Med Chem - Anti-Inflammatory \& Anti-Allergy Agents, 3, pp. 303-311; 
Buescher, J., Margaritis, A., Microbial biosynthesis of polyglutamic acid biopolymer and applications in the biopharmaceutical, biomedical and food industries (2007) Crit Rev Biotechnol, 27, pp. 1-19; 
Chi, P., Wang, J., Liu, C., Synthesis and characterization of polycationic chitosan-graft-poly (L-lysine) (2008) Mat Lett, 62, pp. 147-150; 
Ciuculescu, F., Giesen, M., Deak, E., Lang, V., Seifried, E., Henschler, R., Variability in chemokine-induced adhesion of human mesenchymal stromal cells (2011) Cytother, 13, pp. 1172-1179; 
Crouzier, T., Ren, K., Nicolas, C., Roy, C., Picart, C., Layer-by-layer films as a biomimetic reservoir for rhBMP-2 delivery: Controlled differentiation of myoblasts to osteoblasts (2009) Small, 5, pp. 598-608; 
de Geest, B., Sanders, N.N., Sukhorukov, G.B., Demeester, J., de Smedt, S.C., Release mechanisms for polyelectrolyte capsules (2007) Chem Soc Rev, 36, pp. 636-649; 
Du, Y.J., Cornelius, R.M., Brash, J.L., Measurement of protein adsorption to gold surface by radioiodination methods: Suppression of free iodide sorption (2000) Colloid Surf B, 17, pp. 59-67; 
Fong, E.L.S., Chan, C.K., Goodman, S.B., Stem cell homing in musculoskeletal injury (2011) Biomaterials, 32, pp. 395-409; 
Frederick, J., Fitzpatrick, J.R., McCormick, R.C., Harris, D.A., Kim, A.Y., Muenzer, J.R., Marotta, N., Woo, Y.J., Stromal cell-derived factor-1alpha activation of tissue-engineered endothelial progenitor cell matrix enhances ventricular function after myocardial infarction by inducing neovasculogenesis (2010) Circulation, 122, pp. S107-S117; 
Gao, H., Priebe, W., Glod, J., Banerjee, D., Activation of signal transducers and activators of transcription 3 and focal adhesion kinase by stromal cell-derived factor 1 is required for migration of human mesenchymal stem cells in response to tumor cell-conditioned medium (2009) Stem Cells, 27, pp. 857-865; 
Gon{\c c}alves, R., Martins, M.C.L., Oliveira, M.J., Almeida-Porada, G., Barbosa, M.A., Bioactivity of immobilized EGF on self-assembled monolayers: Optimization of the immobilization process (2010) J Biomed Mater Res Part A, 94 A, pp. 576-585; 
Granero-Molt{\'o}, F., Weis, J.A., Miga, M.I., Landis, B., Myers, T.J., O{\textquoteright}Rear, L., Longobardi, L., Spagnoli, A., Regenerative effects of transplanted mesenchymal stem cells in fracture healing (2009) Stem Cells, 27, pp. 1887-1898; 
Haider, S., Al-Masry, W.A., Bukhari, N., Javid, M., Preparation of the chitosan containing nanofibers by electrospinning chitosan-gelatin complexes (2010) Polymer Eng Sci, 50, pp. 1887-1893; 
Higuchi, T., Mechanism of sustained-action medication. Theoretical analysis of rate of release of solid drugs dispersed in solid matrices (1963) J Pharm Sci, 52, pp. 1145-1149; 
Hofmann, S., Knecht, S., Langer, R., Kaplan, D.L., Vunjak-Novakovic, G., Merkle, H.P., Meinel, L., Cartilage-like tissue engineering using silk scaffolds and mesenchymal stem cells (2006) Tissue Eng, 12, pp. 2729-2738; 
Horbett, T., Techniques for protein adsorption studies (1986) Techniques of Biocompatibility Tests, pp. 183-214. , Williams DF, ed), CRC Press, Boca Raton, FL; 
Hsieh, C., Hsieh, H.J., Liu, H.C., Wang, D.M., Hou, L.T., Fabrication and release behavior of a novel freezegelled chitosan/γ-PGA scaffold as a carrier for rhBMP-2 (2006) Dental Mater, 22, pp. 622-629; 
Hsieh, C., Tsai, S.P., Wang, D.M., Chang, Y.N., Hsieh, H.J., Preparation of PGA/chitosan composite tissue engineering matrices (2005) Biomaterials, 26, pp. 5617-5623; 
Huang, M., Vitharana, S.N., Peek, L.J., Coop, T., Berkland, C., Polyelectrolyte complexes stabilize and controllably release Vascular Endothelial Growth Factor (2007) Biomacromolecules, 8, pp. 1607-1614; 
Ichim, T., Alexandrescu, D.U., Solano, F., Lara, F., Campion, R.N., Paris, E., Woods, E.J., Riordan, N.H., Mesenchymal stem cells as anti-inflammatories: Implications for treatment of Duchenne muscular dystrophy (2010) Cell Immunol, 260, pp. 75-82; 
Kim, C., Broxmeyer, H.E., In vitro behavior of hematopoietic progenitor cells under the influence of chemoattractants: Stromal cell-derived factor-1, Steel factor, and the bone marrow environment (1998) Blood, 91, pp. 100-110; 
Kim, M., Koh, Y.J., Kim, K.E., Koh, B.I., Nam, D.H., Alitalo, K., Kim, I., Koh, G.Y., CXCR4 signaling regulates metastasis of chemoresistant melanoma cells by a lymphatic metastatic niche (2010) Cancer Res, 70, pp. 10411-10421; 
Kuraitis, D.Z.P., Zhang, Y., Padavan, D.T., McEwan, K., Sofrenovic, T., McKee, D., Zhang, J., Suuronen, E.J., Controlled release of stromal cell-derived factor-1 from a collagen matrix for enhanced progenitor cell response and blood vessel growth in ischemia (2011) Eur Cell Mater, 5, pp. 109-123; 
Li, T., Li, H., Wang, Y., Harvard, C., Tan, J.L., Au, A., Xu, Z., You, L., The expression of CXCR4, CXCL12 and CXCR7 in malignant pleural mesothelioma (2011) J Pathol, 223, pp. 519-530; 
Liu, H., Xue, W., Ge, G., Luo, X., Li, Y., Xiang, H., Ding, X., Tian, X., Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1a in MSCs (2010) Biochem Biophys Res Commun, 401, pp. 509-515; 
Macdonald, M., Samuel, R.E., Shah, N.J., Padera, R.F., Beben, Y.M., Hammond, P.T., Tissue integration of growth factor-eluting layer-by-layer polyelectrolyte multilayer coated implants (2011) Biomaterials, 32, pp. 1446-1453; 
Lda, M., Fontes, A.M., Covas, D.T., Caplan, A.I., Mechanisms involved in the therapeutic properties of mesenchymal stem cells (2009) Cytokine Growth Factor Rev, 20, pp. 419-427; 
Mokashi, S.A., Guan, J., Wang, D., Tchantchaleishvili, V., Brigham, M., Lipsitz, S., Lee, L.S., Chen, F.Y., Preventing cardiac remodeling: The combination of cell-based therapy and cardiac support therapy preserves left ventricular function in rodent model of myocardial ischemia (2010) J Thoracic Cardiovasc Surg, 140, pp. 1374-1380; 
Oliveira, S.M., Mijares, D.Q., Turner, G., Amaral, I.F., Barbosa, M.A., Teixeira, C.C., Engineering endochondral bone: In vivo studies (2009) Tissue Eng A, 15, pp. 635-643; 
Peled, A., Petit, I., Kollet, O., Magid, M., Ponomaryov, T., Byk, T., Nagler, A., Lapidot, T., Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4 (1999) Science, 283, pp. 845-848; 
Picart, C., Mutterer, J., Richer, L., Luo, Y., Prestwich, G.D., Schaaf, P., Voegel, J.C., Lavalle, P., Molecular basis for the explanation of the exponential growth of polyelectrolyte multilayers (2002) Proc Natl Acad Sci USA, 99, pp. 12531-12535; 
Ponte, A., Marais, E., Gallay, N., Langonne, A., Delorme, B., Herault, E., Charboard, P., Domenesh, J., The in vitro migration capacity of human bone marrow mesenchymal stem cells: Comparison of chemokine and growth factor chemotactic activities (2009) Stem Cells, 25, pp. 1737-1745; 
Rabbany, S., Pastore, J., Yamamoto, M., Miller, T., Rafii, S., Aras, R., Penn, M., Continuous delivery of stromal cell-derived factor-1 from alginate scaffolds accelerates wound healing (2010) Cell Transplant, 19, pp. 399-408; 
Richert, L., Lavalle, P., Payan, E., Shu, X.Z., Prestwich, G.D., Stoltz, J.F., Schaaf, P., Picart, C., Layer by layer buildup of polysaccharide films: Physical chemistry and cellular adhesion aspects (2003) Langmuir, 20, pp. 448-458; 
Ruster, B., Grace, B., Seitza, O., Seifried, E., Henschler, R., Induction and detection of human mesenchymal stem cell migration in the 48-well reusable Transwell assay (2005) Stem Cells Dev, 14, pp. 231-235; 
Schneider, R.K., Anraths, J., Kramann, R., Bornemann, J., Bovi, M., Kn{\"o}chel, R., Neuss, S., The role of biomaterials in the direction of mesenchymal stem cell properties and extracellular matrix remodelling in dermal tissue engineering (2010) Biomaterials, 31, pp. 7948-7959; 
Shen, W., Chen, X., Chen, J., Yin, Z., Heng, B.C., Chen, W., Ouyang, H.W., The effect of incorporation of exogenous stromal cell-derived factor-1 alpha within a knitted silk-collagen sponge in tendon regeneration (2010) Biomaterials, 31, pp. 7239-7249; 
Shinohara, K., Greenfield, S., Pan, H., Vasanji, A., Kumagai, K., Midura, R.J., Kiedrowski, M., Muschler, G.F., Stromal cell-derived factor-1 and monocyte chemotactic protein-3 improve recruitment of osteogenic cells into sites of musculoskeletal repair (2011) J Orthop Res, 29, pp. 1064-1069; 
Singer, N., Caplan, A.I., Mesenchymal stem cells: Mechanisms of inflammation (2011) Annu Rev Pathol Mech Dis, 6, pp. 457-478; 
Song, Z., Yin, J., Luo, K., Zheng, Y., Yang, Y., Li, Q., Yan, S., Chen, X., Layer-by-layer buildup of poly(Lglutamic acid)/chitosan film for biologically active coating (2009) Macromol Biosci, 9, pp. 268-278; 
Sundelacruz, S., Kaplan, D.L., Stem celland scaffold-based tissue engineering approaches to osteochondral regenerative medicine (2009) Sem Cell Dev Biol, 20, pp. 646-655; 
Tang, J., Wang, J.N., Zhang, L., Zheng, F., Yang, J.Y., Kong, X., Guo, L.Y., Chen, S.Y., VEGF/SDF-1 promotes cardiac stem cell mobilization and myocardial repair in the infarcted heart (2011) Cardiovasc Res, 91, pp. 402-411; 
Thevenot, P., Nair, A.M., Shen, J., Lotfi, P., Ko, C.Y., Tang, L., The effect of incorporation of SDF-1a into PLGA scaffolds on stem cell recruitment and the inflammatory response (2010) Biomaterials, 31, pp. 3997-4008; 
Thieme, S., Ryser, M., Gentsch, M., Navratiel, K., Brenner, S., Stiehler, M., Rolfing, J., Rosen-Wolff, A., Stromal cell-derived factor-1a-directed chemoattraction of transiently CXCR4-overexpressing bone marrow stromal cells into functionalized three dimensional biomimetic scaffolds (2009) Tissue Eng C, 15, pp. 687-696; 
Uccelli, A., Moretta, L., Pistoia, V., Mesenchymal stem cells in health and disease (2008) Nat Rev Immunol, 8, pp. 726-736; 
Wu, M.Y., Chen, N., Liu, L.K., Yuan, H., Li, Q.L., Chen, S.H., Chitosan/alginate multilayer scaffold encapsulating bone marrow stromal cells in situ on titanium (2009) J Bioact Compat Polym, 24, pp. 301-315; 
Wynn, R., Hart, C.A., Corradi-Perini, C., O{\textquoteright}Neill, L., Evans, C.A., Wraith, J.E., Fairbairn, L.J., Bellantuono, I., A small proportion of mesenchymal stem cells strongly expresses functionally active CXCR4 receptor capable of promoting migration to bone marrow (2004) Blood, 104, pp. 2643-2645; 
Yoo, K., Jang, I.K., Lee, M.W., Kim, H.E., Yang, M.S., Eomb, Y., Lee, J.E., Koo, H.H., Comparison of immunomodulatory properties of mesenchymal stem cells derived from adult human tissues (2009) Cellr Immunol, 259, pp. 150-156; 
Zhukareva, V., Obrocka, M., Houle, J.D., Fischer, I., Neuhuber, B., Secretion profile of human bone marrow stromal cells: Donor variability and response to inflammatory stimuli (2010) Cytokine, 50, pp. 317-321
},
	month = {2012///},
	pages = {249 - 261},
	abstract = {Human mesenchymal stem cells (hMSCs) have an enormous potential for tissue engineering and cell-based therapies. With a potential of differentiation into multiple lineages and immune-suppression, these cells play a key role in tissue remodelling and regeneration. Here a method of hMSC recruitment is described, based on the incorporation of a chemokine in Chitosan (Ch)/ Poly(γ-glutamic acid) (γ-PGA) complexes. Ch is a nontoxic, cationic polysaccharide widely investigated. γ-PGA is a hydrophilic, non-toxic, biodegradable and negatively charged poly-amino acid. Ch and γ-PGA, being oppositely charged, can be combined through electrostatic interactions. These biocompatible structures can be used as carriers for active substances and can be easily modulated in order to control the delivery of drugs, proteins, DNA, etc. Using the layer-by-layer method, Ch and γ-PGA were assembled into polyelectrolyte multilayers films (PEMs) with thickness of 120 nm. The chemokine stromal-derived factor-1 (SDF-1) was incorporated in these complexes and was continuously released during 120 h. The method of SDF-1 incorporation is of crucial importance for polymers assembly into PEMs and for the release kinetics of this chemokine. The Ch/γ-PGA PEMs with SDF-1 were able to recruit hMSCs, increasing the cell migration up to 6 fold to a maximum of 16.2 {\textpm} 4.9 cells/mm                        2. The controlled release of SDF-1 would be of great therapeutic value in the process of hMSC homing to injured tissues. This is the first study suggesting Ch/γ-PGA PEMs as SDF-1 reservoirs to recruit hMSCs, describing an efficient method of chemokine incorporation that allows a sustained released up to 5 days and that can be easily scaled-up.
},
	keywords = {adsorption kinetics, article, artificial membrane, Biocompatible Materials, biomaterial, cell differentiation, cell migration, cell motion, Cell Movement, Cell recruitment, chemistry, Chemokine CXCL12, chitosan, controlled study, CXCL12 protein, human, cytology, drug adsorption, drug delivery system, Drug Delivery Systems, drug derivative, drug effect, ellipsometry, enzyme linked immunosorbent assay, human, human cell, human tissue, Humans, in vitro study, isotope labeling, Layer-by-layer, Membranes, Artificial, Mesenchymal stem cell, Mesenchymal Stem Cells, metabolism, methodology, poly(gamma glutamic acid), poly(gamma-glutamic acid), Poly(γ-glutamic acid), polyglutamic acid, Protein delivery, static electricity, stromal cell derived factor 1, sustained drug release},
	isbn = {14732262 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864582297\&partnerID=40\&md5=2fe4a066582cc820d2a8f9dd64fde21b},
	author = {Gon{\c c}alves, R.M. and Antunes, J.C. and Barbosa, M.A.}
}
@article {2236,
	title = {Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {7},
	year = {2011},
	note = {Cited By (since 1996): 24Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2010.11.005PubMed ID: 21056701Language of Original Document: EnglishCorrespondence Address: Martins, M. C. L.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade Do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: cmartins@ineb.up.ptChemicals/CAS: amoxicillin, 26787-78-0, 34642-77-8, 61336-70-7; ampicillin, 69-52-3, 69-53-4, 7177-48-2, 74083-13-9, 94586-58-0; carbenicillin, 17230-86-3, 4697-36-3, 4800-94-6; cefazolin, 25953-19-9, 27164-46-1; gentamicin, 1392-48-9, 1403-66-3, 1405-41-0; penicillin G, 1406-05-9, 61-33-6; teicoplanin, 61036-62-2, 61036-64-4; tobramycin, 32986-56-4; vancomycin, 1404-90-6, 1404-93-9; Antimicrobial Cationic Peptides; Biocompatible Materials; Coated Materials, Biocompatible; Immobilized ProteinsReferences: Glinel, K., Jonas, A.M., Jouenne, T., Leprince, J., Galas, L., Huck, W.T., Antibacterial and antifouling polymer brushes incorporating antimicrobial peptide (2009) Bioconj Chem, 20 (1), pp. 71-77;Vila, J., Soriano, A., Mensa, J., Molecular basis of microbial adherence to prosthetic materials. Role of biofilms in prosthesis-associated infection (2008) Enferm Infecc Microbiol Clin, 26 (1), pp. 48-54; 
Hetrick, E.M., Schoenfisch, M.H., Reducing implant-related infections: Active release strategies (2006) Chemical Society Reviews, 35 (9), pp. 780-789. , DOI 10.1039/b515219b; 
Darouiche, R.O., Treatment of Infections Associated with Surgical Implants (2004) New England Journal of Medicine, 350 (14), pp. 1422-1429. , DOI 10.1056/NEJMra035415; 
Zhao, L., Chu, P.K., Zhang, Y., Wu, Z., Antibacterial coatings on titanium implants (2009) J Biomed Mater Res B Appl Biomater, 91 (1), pp. 470-480; 
Dee, K.C., Puleo, D.A., Bizios, R., (2002) An Introduction to Tissue-biomaterial Interactions, , Wiley-Liss New York; 
Subramani, K., Jung, R.E., Molenberg, A., Hammerle, C.H., Biofilm on dental implants: A review of the literature (2009) Int J Oral Maxillofac Implants, 24 (4), pp. 616-626; 
Fey, P.D., Olson, M.E., Current concepts in biofilm formation of Staphylococcus epidermidis (2010) Future Microbiol, 5 (6), pp. 917-933; 
De Carvalho, C.C., Biofilms: Recent developments on an old battle (2007) Recent Pat Biotechnol, 1 (1), pp. 49-57; 
Costerton, J.W., Stewart, P.S., Greenberg, E.P., Bacterial biofilms: A common cause of persistent infections (1999) Science, 284 (5418), pp. 1318-1322. , DOI 10.1126/science.284.5418.1318; 
Willcox, M.D., Hume, E.B., Aliwarga, Y., Kumar, N., Cole, N., A novel cationic-peptide coating for the prevention of microbial colonization on contact lenses (2008) J Appl Microbiol, 105 (6), pp. 1817-1825; 
Lewis, K., Riddle of biofilm resistance (2001) Antimicrobial Agents and Chemotherapy, 45 (4), pp. 999-1007. , DOI 10.1128/AAC.45.4.999-1007.2001; 
Monteiro, D.R., Gorup, L.F., Takamiya, A.S., Ruvollo-Filho, A.C., De Camargo, E.R., Barbosa, D.B., The growing importance of materials that prevent microbial adhesion: Antimicrobial effect of medical devices containing silver (2009) Int J Antimicrob Agents, 34 (2), pp. 103-110; 
Gooderham, W.J., Bains, M., McPhee, J.B., Wiegand, I., Hancock, R.E., Induction by cationic antimicrobial peptides and involvement in intrinsic polymyxin and antimicrobial peptide resistance, biofilm formation, and swarming motility of PsrA in Pseudomonas aeruginosa (2008) J Bacteriol, 190 (16), pp. 5624-5634; 
Bagge, N., Schuster, M., Hentzer, M., Ciofu, O., Givskov, M., Greenberg, E.P., Hoiby, N., Pseudomonas aeruginosa Biofilms Exposed to Imipenem Exhibit Changes in Global Gene Expression and β-Lactamase and Alginate Production (2004) Antimicrobial Agents and Chemotherapy, 48 (4), pp. 1175-1187. , DOI 10.1128/AAC.48.4.1175-1187.2004; 
Ahmed, N.A., Petersen, F.C., Scheie, A.A., AI-2/LuxS is involved in increased biofilm formation by Streptococcus intermedius in the presence of antibiotics (2009) Antimicrob Agents Chemother, 53 (10), pp. 4258-4263; 
Hoffman, L.R., D{\textquoteright}Argenio, D.A., MacCoss, M.J., Zhang, Z., Jones, R.A., Miller, S.I., Aminoglycoside antibiotics induce bacterial biofilm formation (2005) Nature, 436 (7054), pp. 1171-1175. , DOI 10.1038/nature03912; 
Harbers, G.M., Emoto, K., Greef, C., Metzger, S.W., Woodward, H.N., Mascali, J.J., Grainger, D.W., Lochhead, M.J., Functionalized poly(ethylene glycol)-based bioassay surface chemistry that facilitates bio-immobilization and inhibits nonspecific protein, bacterial, and mammalian cell adhesion (2007) Chemistry of Materials, 19 (18), pp. 4405-4414. , DOI 10.1021/cm070509u; 
Chapman, R.G., Ostuni, E., Liang, M.N., Meluleni, G., Kim, E., Yan, L., Pier, G., Whitesides, G.M., Polymeric thin films that resist the adsorption of proteins and the adhesion of bacteria (2001) Langmuir, 17 (4), pp. 1225-1233. , DOI 10.1021/la001222d; 
Goncalves, I.C., Martins, M.C., Barbosa, M.A., Naeemi, E., Ratner, B.D., Selective protein adsorption modulates platelet adhesion and activation to oligo(ethylene glycol)-terminated self-assembled monolayers with C18 ligands (2009) J Biomed Mater Res A, 89 (3), pp. 642-653; 
Chung, D., Papadakis, S.E., Yam, K.L., Evaluation of a polymer coating containing triclosan as the antimicrobial layer for packaging materials (2003) International Journal of Food Science and Technology, 38 (2), pp. 165-169. , DOI 10.1046/j.1365-2621.2003.00657.x; 
Leung, D., Spratt, D.A., Pratten, J., Gulabivala, K., Mordan, N.J., Young, A.M., Chlorhexidine-releasing methacrylate dental composite materials (2005) Biomaterials, 26 (34), pp. 7145-7153. , DOI 10.1016/j.biomaterials.2005.05.014, PII S0142961205003881; 
Ravikumar, T., Murata, H., Koepsel, R.R., Russell, A.J., Surface-active antifungal polyquaternary amine (2006) Biomacromolecules, 7 (10), pp. 2762-2769. , DOI 10.1021/bm060476w; 
Bach, A., Darby, D., Bottiger, B., Bohrer, H., Motsch, J., Martin, E., Retention of the antibiotic teicoplanin on a hydromer-coated central venous catheter to prevent bacterial colonization in postoperative surgical patients (1996) Intensive Care Medicine, 22 (10), pp. 1066-1069. , DOI 10.1007/s001340050214; 
Jose, B., Antoci Jr., V., Zeiger, A.R., Wickstrom, E., Hickok, N.J., Vancomycin covalently bonded to titanium beads kills Staphylococcus aureus (2005) Chemistry and Biology, 12 (9), pp. 1041-1048. , DOI 10.1016/j.chembiol.2005.06.013, PII S1074552105002309; 
Kilic, D., Agalar, C., Ozturk, E., Denkbas, E.B., Cime, A., Agalar, F., Antimicrobial activity of cefazolin-impregnated mesh grafts (2007) ANZ Journal of Surgery, 77 (4), pp. 256-260. , DOI 10.1111/j.1445-2197.2007.04029.x; 
Gallardo-Moreno, A.M., Pacha-Olivenza, M.A., Saldana, L., Perez-Giraldo, C., Bruque, J.M., Vilaboa, N., In vitro biocompatibility and bacterial adhesion of physico-chemically modified Ti6Al4V surface by means of UV irradiation (2009) Acta Biomater, 5 (1), pp. 181-192; 
Jansen, B., Kohnen, W., Prevention of biofilm formation by polymer modification (1995) Journal of Industrial Microbiology, 15 (4), pp. 391-396. , DOI 10.1007/BF01569996; 
Martins, M.C.L., Wang, D., Ji, J., Feng, L., Barbosa, M.A., Albumin and fibrinogen adsorption on Cibacron blue F3G-A immobilised onto PU-PHEMA (polyurethane-poly (hydroxyethylmethacrylate)) surfaces (2003) Journal of Biomaterials Science, Polymer Edition, 14 (5), pp. 439-455. , DOI 10.1163/156856203766652057; 
Zhang, F., Zhang, Z., Zhu, X., Kang, E.T., Neoh, K.G., Silk-functionalized titanium surfaces for enhancing osteoblast functions and reducing bacterial adhesion (2008) Biomaterials, 29 (36), pp. 4751-4759; 
Flemming, R.G., Capelli, C.C., Cooper, S.L., Proctor, R.A., Bacterial colonization of functionalized polyurethanes (2000) Biomaterials, 21 (3), pp. 273-281; 
Singla, A.K., Chawla, M., Chitosan: Some pharmaceutical and biological aspects - An update (2001) Journal of Pharmacy and Pharmacology, 53 (8), pp. 1047-1067. , DOI 10.1211/0022357011776441; 
Murata, H., Koepsel, R.R., Matyjaszewski, K., Russell, A.J., Permanent, non-leaching antibacterial surfaces-2: How high density cationic surfaces kill bacterial cells (2007) Biomaterials, 28 (32), pp. 4870-4879. , DOI 10.1016/j.biomaterials.2007.06.012, PII S0142961207004681; 
Aumsuwan, N., Danyus, R.C., Heinhorst, S., Urban, M.W., Attachment of ampicillin to expanded poly(tetrafluoroethylene): Surface reactions leading to inhibition of microbial growth (2008) Biomacromolecules, 9 (7), pp. 1712-1718; 
Cevher, E., Orhan, Z., Mulazimoglu, L., Sensoy, D., Alper, M., Yildiz, A., Characterization of biodegradable chitosan microspheres containing vancomycin and treatment of experimental osteomyelitis caused by methicillin-resistant Staphylococcus aureus with prepared microspheres (2006) Int J Pharm, 317 (2), pp. 127-135; 
Faber, C., Stallmann, H.P., Lyaruu, D.M., Joosten, U., Von Eiff, C., Van Nieuw Amerongen, A., Wuisman, P.I.J.M., Comparable efficacies of the antimicrobial peptide human lactoferrin 1-11 and gentamicin in a chronic methicillin-resistant Staphylococcus aureus osteomyelitis model (2005) Antimicrobial Agents and Chemotherapy, 49 (6), pp. 2438-2444. , DOI 10.1128/AAC.49.6.2438-2444.2005; 
Aumsuwan, N., Heinhorst, S., Urban, M.W., Antibacterial surfaces on expanded polytetrafluoroethylene; penicillin attachment (2007) Biomacromolecules, 8 (2), pp. 713-718. , DOI 10.1021/bm061050k; 
Palumbi, S.R., Humans as the world{\textquoteright}s greatest evolutionary force (2001) Science, 293 (5536), pp. 1786-1790. , DOI 10.1126/science.293.5536.1786; 
Andreu, D., Rivas, L., Animal antimicrobial peptides: An overview (1998) Biopolymers, 47 (6), pp. 415-433; 
Rivas, L., Luque-Ortega, J.R., Andreu, D., Amphibian antimicrobial peptides and Protozoa: Lessons from parasites (2009) Biochim Biophys Acta, 1788 (8), pp. 1570-1581; 
Gordon, Y.J., Romanowski, E.G., McDermott, A.M., Mini review: A review of antimicrobial peptides and their therapeutic potential as anti-infective drugs (2005) Current Eye Research, 30 (7), pp. 505-515. , DOI 10.1080/02713680590968637; 
Guani-Guerra, E., Santos-Mendoza, T., Lugo-Reyes, S.O., Teran, L.M., Antimicrobial peptides: General overview and clinical implications in human health and disease (2010) Clin Immunol, 135 (1), pp. 1-11; 
Perron, G.G., Zasloff, M., Bell, G., Experimental evolution of resistance to an antimicrobial peptide (2006) Proceedings of the Royal Society B: Biological Sciences, 273 (1583), pp. 251-256. , DOI 10.1098/rspb.2005.3301; 
Reddy, K.V.R., Yedery, R.D., Aranha, C., Antimicrobial peptides: Premises and promises (2004) International Journal of Antimicrobial Agents, 24 (6), pp. 536-547. , DOI 10.1016/j.ijantimicag.2004.09.005, PII S092485790400322X; 
Van {\textquoteright}T Hof, W., Veerman, E.C.I., Heimerhorst, E.J., Nieuw Amerongen, A.V., Antimicrobial peptides: Properties and applicability (2001) Biological Chemistry, 382 (4), pp. 597-619. , DOI 10.1515/BC.2001.072; 
Li, M., Cha, D.J., Lai, Y., Villaruz, A.E., Sturdevant, D.E., Otto, M., The antimicrobial peptide-sensing system aps of Staphylococcus aureus (2007) Molecular Microbiology, 66 (5), pp. 1136-1147. , DOI 10.1111/j.1365-2958.2007.05986.x; 
Li, M., Lai, Y., Villaruz, A.E., Cha, D.J., Sturdevant, D.E., Otto, M., Gram-positive three-component antimicrobial peptide-sensing system (2007) Proceedings of the National Academy of Sciences of the United States of America, 104 (22), pp. 9469-9474. , DOI 10.1073/pnas.0702159104; 
Otto, M., Bacterial sensing of antimicrobial peptides (2009) Contrib Microbiol, 16, pp. 136-149; 
Peschel, A., Vincent Collins, L., Staphylococcal resistance to antimicrobial peptides of mammalian and bacterial origin (2001) Peptides, 22 (10), pp. 1651-1659. , DOI 10.1016/S0196-9781(01)00500-9, PII S0196978101005009; 
Peschel, A., Jack, R.W., Otto, M., Collins, L.V., Staubitz, P., Nicholson, G., Kalbacher, H., Van Strijp, J.A.G., Staphylococcus aureus resistance to human defensins and evasion of neutrophil killing via the novel virulence factor MprF is based on modification of membrane lipids with L-lysine (2001) Journal of Experimental Medicine, 193 (9), pp. 1067-1076. , DOI 10.1084/jem.193.9.1067; 
Cao, M., Helmann, J.D., The Bacillus subtilis Extracytoplasmic-Function σ X Factor Regulates Modification of the Cell Envelope and Resistance to Cationic Antimicrobial Peptides (2004) Journal of Bacteriology, 186 (4), pp. 1136-1146. , DOI 10.1128/JB.186.4.1136-1146.2004; 
Clarke, S.R., Foster, S.J., IsdA protects Staphylococcus aureus against the bactericidal protease activity of apolactoferrin (2008) Infection and Immunity, 76 (4), pp. 1518-1526. , DOI 10.1128/IAI.01530-07; 
Vuong, C., Voyich, J.M., Fischer, E.R., Braughton, K.R., Whitney, A.R., DeLeo, F.R., Otto, M., Polysaccharide intercellular adhesin (PIA) protects Staphylococcus epidermidis against major components of the human innate immune system (2004) Cellular Microbiology, 6 (3), pp. 269-275. , DOI 10.1046/j.1462-5822.2004.00367.x; 
Zasloff, M., Antimicrobial peptides of multicellular organisms (2002) Nature, 415 (6870), pp. 389-395. , DOI 10.1038/415389a; 
Hancock, R.E.W., Peptide antibiotics (1997) Lancet, 349 (9049), pp. 418-422. , DOI 10.1016/S0140-6736(97)80051-7; 
Cho, W.-M., Joshi, B.P., Cho, H., Lee, K.-H., Design and synthesis of novel antibacterial peptide-resin conjugates (2007) Bioorganic and Medicinal Chemistry Letters, 17 (21), pp. 5772-5776. , DOI 10.1016/j.bmcl.2007.08.056, PII S0960894X0701013X; 
Statz, A.R., Park, J.P., Chongsiriwatana, N.P., Barron, A.E., Messersmith, P.B., Surface-immobilised antimicrobial peptoids (2008) Biofouling, 24 (6), pp. 439-448; 
Deslouches, B., Gonzalez, I.A., DeAlmeida, D., Islam, K., Steele, C., Montelaro, R.C., Mietzner, T.A., De novo-derived cationic antimicrobial peptide activity in a murine model of Pseudomonas aeruginosa bacteraemia (2007) Journal of Antimicrobial Chemotherapy, 60 (3), pp. 669-672. , DOI 10.1093/jac/dkm253; 
Novak, K.F., Diamond, W.J., Kirakodu, S., Peyyala, R., Anderson, K.W., Montelaro, R.C., Mietzner, T.A., Efficacy of the de novo-derived antimicrobial peptide WLBU2 against oral bacteria (2007) Antimicrobial Agents and Chemotherapy, 51 (5), pp. 1837-1839. , DOI 10.1128/AAC.00924-06; 
Deslouches, B., Islam, K., Craigo, J.K., Paranjape, S.M., Montelaro, R.C., Mietzner, T.A., Activity of the de novo engineered antimicrobial peptide WLBU2 against Pseudomonas aeruginosa in human serum and whole blood: Implications for systemic applications (2005) Antimicrobial Agents and Chemotherapy, 49 (8), pp. 3208-3216. , DOI 10.1128/AAC.49.8.3208-3216.2005; 
Deslouches, B., Phadke, S.M., Lazarevic, V., Cascio, M., Islam, K., Montelaro, R.C., Mietzner, T.A., De novo generation of cationic antimicrobial peptides: Influence of length and tryptophan substitution on antimicrobial activity (2005) Antimicrobial Agents and Chemotherapy, 49 (1), pp. 316-322. , DOI 10.1128/AAC.49.1.316-322.2005; 
Levin, M., Quint, P.A., Goldstein, B., Barton, P., Bradley, J.S., Shemie, S.D., Recombinant bactericidal/permeability-increasing protein (rBPI21) as adjunctive treatment for children with severe meningococcal sepsis: A randomised trial. rBPI21 Meningococcal Sepsis Study Group (2000) Lancet, 356 (9234), pp. 961-967; 
Giroir, B.P., Scannon, P.J., Levin, M., Bactericidal/permeability-increasing protein-lessons learned from the phase III, randomized, clinical trial of rBPI21 for adjunctive treatment of children with severe meningococcemia (2001) Crit Care Med, 29 (7 SUPPL.), pp. 130-135; 
Brogden, K.A., Antimicrobial peptides: Pore formers or metabolic inhibitors in bacteria? (2005) Nature Reviews Microbiology, 3 (3), pp. 238-250. , DOI 10.1038/nrmicro1098; 
Bastos, M., Bai, G., Gomes, P., Andreu, D., Goormaghtigh, E., Prieto, M., Energetics and partition of two cecropin-melittin hybrid peptides to model membranes of different composition (2008) Biophys J, 94 (6), pp. 2128-2141; 
Melo, M.N., Ferre, R., Castanho, M.A., Antimicrobial peptides: Linking partition, activity and high membrane-bound concentrations (2009) Nat Rev Microbiol, 7 (3), pp. 245-250; 
Haynie, S.L., Crum, G.A., Doele, B.A., Antimicrobial activities of amphiphilic peptides covalently bonded to a water-insoluble resin (1995) Antimicrob Agents Chemother, 39 (2), pp. 301-307; 
Humblot, V., Yala, J.F., Thebault, P., Boukerma, K., Hequet, A., Berjeaud, J.M., The antibacterial activity of Magainin i immobilized onto mixed thiols self-assembled monolayers (2009) Biomaterials, 30 (21), pp. 3503-3512; 
Bagheri, M., Beyermann, M., Dathe, M., Immobilization reduces the activity of surface-bound cationic antimicrobial peptides with no influence upon the activity spectrum (2009) Antimicrob Agents Chemother, 53 (3), pp. 1132-1141; 
Gabriel, M., Nazmi, K., Veerman, E.C., Nieuw Amerongen, A.V., Zentner, A., Preparation of LL-37-grafted titanium surfaces with bactericidal activity (2006) Bioconj Chem, 17 (2), pp. 548-550; 
Hilpert, K., Elliott, M., Jenssen, H., Kindrachuk, J., Fjell, C.D., Korner, J., Screening and characterization of surface-tethered cationic peptides for antimicrobial activity (2009) Chem Biol, 16 (1), pp. 58-69; 
Batista, M.K.S., Gallemi, M., Adeva, A., Gomes, C.A.R., Gomes, P., Facile regioselective synthesis of a novel chitosan-pexiganan conjugate with potential interest for the treatment of infected skin lesions (2009) Synthetic Commun, 39 (7), pp. 1228-1240; 
Venter, J.C., Immobilized and insolubilized drugs, hormones, and neurotransmitters: Properties, mechanisms of action and applications (1982) Pharmacological Reviews, 34 (2), pp. 153-187; 
Endo, Y., Tani, T., Kodama, M., Antimicrobial activity of tertiary amine covalently bonded to a polystyrene fiber (1987) Appl Environ Microbiol, 53 (9), pp. 2050-2055; 
Chuang, H.F., Shukla, A., Loose, C.R., Hammond-Cunningham, P.T., Stephanopoulos, G., (2009) Structures Including Antimicrobial Peptides, , United States; 
Chen, R., Cole, N., Willcox, M.D., Park, J., Rasul, R., Carter, E., Synthesis, characterization and in vitro activity of a surface-attached antimicrobial cationic peptide (2009) Biofouling, 25 (6), pp. 517-524; 
Steven, M.D., Hotchkiss, J.H., Covalent immobilization of an antimicrobial peptide on poly(ethylene) film (2008) J Appl Polym Sci, 110 (5), pp. 2665-2670; 
Appendini, P., Hotchkiss, J.H., Surface modification of poly(styrene) by the attachment of an antimicrobial peptide (2001) J Appl Polym Sci, 81 (3), pp. 609-616; 
Fields, G.B., Noble, R.L., Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids (1990) International Journal of Peptide and Protein Research, 35 (3), pp. 161-214; 
Rostovtsev, V.V., Green, L.G., Fokin, V.V., Sharpless, K.B., A stepwise huisgen cycloaddition process: Copper(I)-catalyzed regioselective "ligation" of azides and terminal alkynes (2002) Angewandte Chemie - International Edition, 41 (14), pp. 2596-2599. , DOI 10.1002/1521-3773(20020715)41: 14<2596::AID-ANIE2596>3.0.CO;2-4; 
Abad, J.M., Velez, M., Santamaria, C., Guisan, J.M., Matheus, P.R., Vazquez, L., Gazaryan, I., Fernandez, V.M., Immobilization of peroxidase glycoprotein on gold electrodes modified with mixed epoxy-boronic acid monolayers (2002) Journal of the American Chemical Society, 124 (43), pp. 12845-12853. , DOI 10.1021/ja026658p; 
Lee, M.-R., Shin, I., Fabrication of chemical microarrays by efficient immobilization of hydrazide-linked substances on epoxide-coated glass surfaces (2005) Angewandte Chemie - International Edition, 44 (19), pp. 2881-2884. , DOI 10.1002/anie.200462720; 
Hudalla, G.A., Murphy, W.L., Immobilization of peptides with distinct biological activities onto stem cell culture substrates using orthogonal chemistries (2010) Langmuir, 26 (9), pp. 6449-6456; 
Cole, N., Hume, E.B., Vijay, A.K., Sankaridurg, P., Kumar, N., Willcox, M.D., In vivo performance of melimine as an antimicrobial coating for contact lenses in models of CLARE and CLPU (2010) Invest Ophthalmol Vis Sci, 51 (1), pp. 390-395
},
	month = {2011///},
	pages = {1431 - 1440},
	abstract = {Bacterial adhesion to biomaterials remains a major problem in the medical devices field. Antimicrobial peptides (AMPs) are well-known components of the innate immune system that can be applied to overcome biofilm-associated infections. Their relevance has been increasing as a practical alternative to conventional antibiotics, which are declining in effectiveness. The recent interest focused on these peptides can be explained by a group of special features, including a wide spectrum of activity, high efficacy at very low concentrations, target specificity, anti-endotoxin activity, synergistic action with classical antibiotics, and low propensity for developing resistance. Therefore, the development of an antimicrobial coating with such properties would be worthwhile. The immobilization of AMPs onto a biomaterial surface has further advantages as it also helps to circumvent AMPs{\textquoteright} potential limitations, such as short half-life and cytotoxicity associated with higher concentrations of soluble peptides. The studies discussed in the current review report on the impact of covalent immobilization of AMPs onto surfaces through different chemical coupling strategies, length of spacers, and peptide orientation and concentration. The overall results suggest that immobilized AMPs may be effective in the prevention of biofilm formation by reduction of microorganism survival post-contact with the coated biomaterial. Minimal cytotoxicity and long-term stability profiles were obtained by optimizing immobilization parameters, indicating a promising potential for the use of immobilized AMPs in clinical applications. On the other hand, the effects of tethering on mechanisms of action of AMPs have not yet been fully elucidated. Therefore, further studies are recommended to explore the real potential of immobilized AMPs in health applications as antimicrobial coatings of medical devices. {\textcopyright} 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.},
	keywords = {Amino Acid Sequence, amoxicillin, ampicillin, Animals, antibiotic agent, antibiotic resistance, Antimicrobial Cationic Peptides, Antimicrobial peptides, Bacteria (microorganisms), Bacterial adhesion, bacterial survival, Biocompatible Materials, biofilm, Biofilms, biomaterial, carbenicillin, cefazolin, Coated Materials, Biocompatible, Cytotoxicity, drug activity, drug half life, drug solubility, drug stability, drug targeting, gentamicin, human, Humans, Immobilized Proteins, Molecular Sequence Data, mouth inflammation, Nanostructured surfaces, nonhuman, penicillin G, polypeptide antibiotic agent, priority journal, review, sepsis, skin infection, soft tissue infection, Surface functionalization, Surface Properties, teicoplanin, tobramycin, vancomycin},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79952187401\&partnerID=40\&md5=70d486a4c6e1ba88f804c0f0d4f4e510},
	author = {Costa, F. and Carvalho, I.F. and Montelaro, R.C. and Gomes, P. and Martins, M.C.L.}
}
@article {2228,
	title = {Isotropic micropatterned silica coatings on zirconia induce guided cell growth for dental implants},
	journal = {Dental MaterialsDent. Mater.},
	volume = {27},
	year = {2011},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: DEMAEdoi: 10.1016/j.dental.2011.02.014Language of Original Document: EnglishCorrespondence Address: Pelaez-Vargas, A.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: alejopv@ineb.up.ptChemicals/CAS: resazurin, 550-82-3; silicon dioxide, 10279-57-9, 14464-46-1, 14808-60-7, 15468-32-3, 60676-86-0, 7631-86-9; yttrium, 7440-65-5; zirconium, 14940-68-2, 7440-67-7; Ceramics; Coated Materials, Biocompatible; Dental Implants; Dental Materials; Indicators and Reagents; Oxazines; Silicon Dioxide, 7631-86-9; Xanthenes; Yttrium, 7440-65-5; Zirconium, 7440-67-7; resazurin, 550-82-3; yttria stabilized tetragonal zirconiaReferences: Kasemo, B., Biocompatibility of titanium implants: Surface science aspects (1983) J Prosthet Dent, 49, pp. 832-837;Egusa, H., Ko, N., Shimazu, T., Yatani, H., Suspected association of an allergic reaction with titanium dental implants: A clinical report (2008) J Prosthet Dent, 100, pp. 344-347; 
Tschernitschek, H., Borchers, L., Geurtsen, W., Nonalloyed titanium as a bioinert metal - A review (2005) Quintessence International, 36 (7), pp. 523-530; 
Oliva, J., Oliva, X., Oliva, J., One-year follow-up of first consecutive 100 zirconia dental implants in human: A comparison of 2 different rough surfaces (2007) Int J Oral Maxillofac Implants, 22, pp. 430-435; 
Johansson, C.B., Hansson, H.A., Albrektsson, T., Qualitative interfacial study between bone and tantalum, niobium or commercially pure titanium (1990) Biomaterials, 11, pp. 277-280; 
De Wijs, F.L., Van Dongen, R.C., De Lange, G.L., De Putter, C., Front tooth replacement with Tubingen (Frialit) implants (1994) J Oral Rehabil, 21, pp. 11-26; 
Albrektsson, T., Lekholm, U., Osseointegration: Current state of the art (1989) Dent Clin North Am, 33, pp. 537-554; 
Albrektsson, T., Sennerby, L., State of the art in oral implants (1991) J Clin Periodontol, 18, pp. 474-481; 
Albrektsson, T., Sennerby, L., Wennerberg, A., State of the art of oral implants (2008) Periodontol 2000, 47, pp. 15-26; 
Sennerby, L., Dental implants: Matters of course and controversies (2008) Periodontol 2000, 47, pp. 9-14; 
Leblebicioglu, B., Rawal, S., Mariotti, A., A review of the functional and esthetic requirements for dental implants (2007) J Am Dent Assoc, 138, pp. 321-329; 
Chevalier, J., What future for zirconia as a biomaterial? (2006) Biomaterials, 27 (4), pp. 535-543. , DOI 10.1016/j.biomaterials.2005.07.034, PII S0142961205007039; 
Hochman, N., Zalkind, M., New all-ceramic indirect post-and-core system (1999) J Prosthet Dent, 81, pp. 625-629; 
Malmstrom, J., Slotte, C., Adolfsson, E., Norderyd, O., Thomsen, P., Bone response to free form-fabricated hydroxyapatite and zirconia scaffolds: A histological study in the human maxilla (2009) Clin Oral Implants Res, 20, pp. 379-385; 
Manicone, P.F., Rossi Iommetti, P., Raffaelli, L., An overview of zirconia ceramics: Basic properties and clinical applications (2007) J Dent, 35, pp. 819-826; 
Kim, H.-W., Kim, H.-E., Salih, V., Knowles, J.C., Dissolution control and cellular responses of calcium phosphate coatings on zirconia porous scaffold (2004) Journal of Biomedical Materials Research - Part A, 68 (3), pp. 522-530; 
Kim, H.W., Shin, S.Y., Kim, H.E., Lee, Y.M., Chung, C.P., Lee, H.H., Bone formation on the apatite-coated zirconia porous scaffolds within a rabbit calvarial defect (2008) J Biomater Appl, 22, pp. 485-504; 
Akagawa, Y., Hosokawa, R., Sato, Y., Kamayama, K., Comparison between freestanding and tooth-connected partially stabilized zirconia implants after two years{\textquoteright} function in monkeys: A clinical and histologic study (1998) J Prosthet Dent, 80, pp. 551-558; 
Kohal, R.J., Att, W., Bachle, M., Butz, F., Ceramic abutments and ceramic oral implants. An update (2008) Periodontol 2000, 47, pp. 224-243; 
Degidi, M., Artese, L., Scarano, A., Perrotti, V., Gehrke, P., Piattelli, A., Inflammatory infiltrate, microvessel density, nitric oxide synthase expression, vascular endothelial growth factor expression, and proliferative activity in peri-implant soft tissues around titanium and zirconium oxide healing caps (2006) Journal of Periodontology, 77 (1), pp. 73-80. , DOI 10.1902/jop.2006.77.1.73; 
Kohal, R.J., Klaus, G., A zirconia implant-crown system: A case report (2004) International Journal of Periodontics and Restorative Dentistry, 24 (2), pp. 147-153; 
Sennerby, L., Dasmah, A., Larsson, B., Iverhed, M., Bone tissue responses to surface-modified zirconia implants: A histomorphometric and removal torque study in the rabbit (2005) Clin Implant Dent Relat Res, 71, pp. 13-20; 
Covacci, V., Bruzzese, N., Maccauro, G., Andreassi, C., Ricci, G.A., Piconi, C., Marmo, E., Cittadini, A., In vitro evaluation of the mutagenic and carcinogenic power of high purity zirconia ceramic (1999) Biomaterials, 20 (4), pp. 371-376. , DOI 10.1016/S0142-9612(98)00182-3, PII S0142961298001823; 
Amaral, M., Costa, M.A., Lopes, M.A., Silva, R.F., Santos, J.D., Fernandes, M.H., Si 3N 4-bioglass composites stimulate the proliferation of MG63 osteoblast-like cells and support the osteogenic differentiation of human bone marrow cells (2002) Biomaterials, 23 (24), pp. 4897-4906. , DOI 10.1016/S0142-9612(02)00249-1, PII S0142961202002491; 
Garvie, R.C., Hannink, R.H., Pascoe, R.T., Ceramic steel? (1975) Nature, 258, pp. 703-704; 
Denry, I., Kelly, J.R., State of the art of zirconia for dental applications (2008) Dent Mater, 24, pp. 299-307; 
Ghosh, A., Suri, A.K., Pandey, M., Thomas, S., Rama Mohan, T.R., Rao, B.T., Nanocrystalline zirconia-yttria system-a Raman study (2006) Materials Letters, 60 (9-10), pp. 1170-1173. , DOI 10.1016/j.matlet.2005.10.102, PII S0167577X05010888; 
Li, M., Feng, Z., Xiong, G., Ying, P., Xin, Q., Li, C., Phase transformation in the surface region of zirconia detected by UV Raman spectroscopy (2001) J Phys Chem B, 105, pp. 8107-8111; 
Lughi, V., Clarke, D.R., Temperature dependence of the yttria-stabilized zirconia Raman spectrum (2007) J Appl Phys, 101; 
Argyriou, D.N., Howard, C.J., Re-investigation of yttria-tetragonal zirconia polycrystal (Y-TZP) by neutron powder diffraction - A cautionary tale (1995) J Appl Crystallogr, 28, pp. 206-208; 
Pascual, C., Duran, P., Subsolidus phase equilibria and ordering in the system ZrO 2-Y 2O 3 (1983) J Am Ceram Soc, 66, p. 23; 
Kelly, J.R., Denry, I., Stabilized zirconia as a structural ceramic: An overview (2008) Dent Mater, 24, pp. 289-298; 
Lughi, V., Sergo, V., Low temperature degradation aging of zirconia: A critical review of the relevant aspects in dentistry (2010) Dent Mater, 26, pp. 807-820; 
Chevalier, J., De Aza, A.H., Fantozzi, G., Schehl, M., Torrecillas, R., Extending the lifetime of ceramic orthopaedic implants (2000) Advanced Materials, 12 (21), pp. 1619-1621. , DOI 10.1002/1521-4095(200011)12:21<1619::AID-ADMA1619>3.0.CO;2-O; 
Kim, H.W., Kong, Y.M., Bae, C.J., Noh, Y.J., Kim, H.E., Sol-gel derived fluoro-hydroxyapatite biocoatings on zirconia substrate (2004) Biomaterials, 25, pp. 2919-2926; 
Lee, E.-J., Lee, S.-H., Kim, H.-W., Kong, Y.-M., Kim, H.-E., Fluoridated apatite coatings on titanium obtained by electron-beam deposition (2005) Biomaterials, 26 (18), pp. 3843-3851. , DOI 10.1016/j.biomaterials.2004.10.019, PII S0142961204009391; 
Vorobyev, A.Y., Guo, C., Femtosecond laser structuring of titanium implants (2007) Applied Surface Science, 253 (17), pp. 7272-7280. , DOI 10.1016/j.apsusc.2007.03.006, PII S0169433207004400; 
Perrie, W., Rushton, A., Gill, M., Fox, P., O{\textquoteright}Neill, W., Femtosecond laser micro-structuring of alumina ceramic (2005) Applied Surface Science, 248 (1-4), pp. 213-217. , DOI 10.1016/j.apsusc.2005.03.005, PII S0169433205003806, 4th International Conference on Photo-Excited Processes and Applications 4-ICEPA; 
Teixeira, S., Monteiro, F.J., Ferraz, M.P., Vilar, R., Eugenio, S., Laser surface treatment of hydroxyapatite for enhanced tissue integration: Surface characterization and osteoblastic interaction studies (2007) Journal of Biomedical Materials Research - Part A, 81 (4), pp. 920-929. , DOI 10.1002/jbm.a.31073; 
Nayab, S.N., Jones, F.H., Olsen, I., Effects of calcium ion implantation on human bone cell interaction with titanium (2005) Biomaterials, 26 (23), pp. 4717-4727. , DOI 10.1016/j.biomaterials.2004.11.044, PII S0142961204010488; 
Kosmac, T., Oblak, C., Jevnikar, P., Funduk, N., Marion, L., The effect of surface grinding and sandblasting on flexural strength and reliability of Y-TZP zirconia ceramic (1999) Dental Materials, 15 (6), pp. 426-433. , PII S0109564199000706; 
Filiaggi, M.J., Pilliar, R.M., Abdulla, D., Evaluating sol-gel ceramic thin films for metal implant applications. II. Adhesion and fatigue properties of zirconia films on Ti-6Al-4V (1996) Journal of Biomedical Materials Research, 33 (4), pp. 239-256. , DOI 10.1002/(SICI)1097-4636(199624)33:4<239::AID-JBM4>3.0.CO;2-Q; 
Sollazzo, V., Pezzetti, F., Scarano, A., Piattelli, A., Bignozzi, C.A., Massari, L., Zirconium oxide coating improves implant osseointegration in vivo (2008) Dent Mater, 24, pp. 357-361; 
Sollazzo, V., Palmieri, A., Pezzetti, F., Bignozzi, C.A., Argazzi, R., Massari, L., Genetic effect of zirconium oxide coating on osteoblast-like cells (2008) J Biomed Mater Res B: Appl Biomater, 84, pp. 550-558; 
Kim, H.-W., Georgiou, G., Knowles, J.C., Koh, Y.-H., Kim, H.-E., Calcium phosphates and glass composite coatings on zirconia for enhanced biocompatibility (2004) Biomaterials, 25 (18), pp. 4203-4213. , DOI 10.1016/j.biomaterials.2003.10.094, PII S0142961203010470; 
Thian, E.S., Huang, J., Best, S.M., Barber, Z.H., Brooks, R.A., Rushton, N., Bonfield, W., The response of osteoblasts to nanocrystalline silicon-substituted hydroxyapatite thin films (2006) Biomaterials, 27 (13), pp. 2692-2698. , DOI 10.1016/j.biomaterials.2005.12.019, PII S0142961205011816; 
Klein, L., Sol-gel processing of silicates (1985) Annu Rev Mater Sci, 15, pp. 227-248; 
McDonagh, C., Sheridan, F., Butler, T., MacCraith, B.D., Characterisation of sol-gel-derived silica films (1996) Journal of Non-Crystalline Solids, 194 (1-2), pp. 72-77; 
Hench, L.L., Sol-gel materials for bioceramic applications (1997) Current Opinion in Solid State and Materials Science, 2 (5), pp. 604-610. , PII S1359028697800538; 
Garcia, C., Cere, S., Duran, A., Bioactive coatings prepared by sol-gel on stainless steel 316L (2004) J Non-Cryst Solids, 348, pp. 218-224; 
Garcia, C., Galliano, P., Cere, S., Electrochemical evaluation of resistance to localised corrosion of vitreous coatings containing particles applied on metallic substrates for biomedical applications (2003) Mater Lett, 57, pp. 1810-1814; 
Xia, Y., Whitesides, G.M., Soft lithography (1998) Angew Chem Int Ed, 37, pp. 550-575; 
Kim, E.J., Boehm, C.A., Mata, A., Fleischman, A.J., Muschler, G.F., Roy, S., Post microtextures accelerate cell proliferation and osteogenesis (2010) Acta Biomater, 6, pp. 160-169; 
Turner, A.M.P., Dowell, N., Turner, S.W.P., Kam, L., Isaacson, M., Turner, J.N., Craighead, H.G., Shain, W., Attachment of astroglial cells to microfabricated pillar arrays of different geometries (2000) Journal of Biomedical Materials Research, 51 (3), pp. 430-441. , DOI 10.1002/1097-4636(20000905)51:3<430::AID-JBM18>3.0.CO;2-C; 
Brunette, D.M., In vitro models of biological responses to implants (1999) Adv Dent Res, 13, p. 35; 
Chen, C.S., Mrksich, M., Huang, S., Whitesides, G.M., Ingber, D.E., Geometric control of cell life and death (1997) Science, 276 (5317), pp. 1425-1428. , DOI 10.1126/science.276.5317.1425; 
Flemming, R.G., Murphy, C.J., Abrams, G.A., Goodman, S.L., Nealey, P.F., Effects of synthetic micro- and nano-structured surfaces on cell behavior (1999) Biomaterials, 20 (6), pp. 573-588. , DOI 10.1016/S0142-9612(98)00209-9, PII S0142961298002099; 
Walboomers, X.F., Jansen, J.A., Cell and tissue behavior on micro-grooved surfaces (2001) Odontology, 89, pp. 2-11; 
Wilkinson, C.D.W., Riehle, M., Wood, M., Gallagher, J., Curtis, A.S.G., The use of materials patterned on a nano- and micro-metric scale in cellular engineering (2002) Materials Science and Engineering C, 19 (1-2), pp. 263-269. , DOI 10.1016/S0928-4931(01)00396-4, PII S0928493101003964, Current trends in Nanotechnologies: From Materials to Systems; 
Bettinger, C.J., Langer, R., Borenstein, J.T., Engineering substrate topography at the micro- and nanoscale to control cell function (2009) Angew Chem Int Ed, 48, pp. 5406-5415; 
Pelaez-Vargas, A., Ferrell, N., Fernandes, M.H., Hansford, D., Monteiro, F.J., Cells spreading on micro-fabricated silica thin film coatings (2009) Microsc Microanal, 15, pp. 77-78; 
Pelaez-Vargas, A., Ferrell, N., Fernandes, M.H., Hansford, D.J., Monteiro, F.J., Cellular alignment induction during early in vitro culture stages using micropatterned glass coatings produced by sol-gel process (2009) Key Eng Mater, 396-398, p. 303; 
Singhvi, R., Stephanopoulos, G., Wang, D.I., Effects of substratum morphology on cell physiology (1994) Biotechnol Bioeng, 43, pp. 764-771; 
Von Recum, A.F., Van Kooten, T.G., The influence of micro-topography on cellular response and the implications for silicone implants (1995) J Biomater Sci Polym Ed, 7, pp. 181-198; 
Pelaez-Vargas, A., Gallego-Perez, D., Ferrell, N., Fernandes, M.H., Hansford, D., Monteiro, F.J., Early spreading and propagation of human bone marrow stem cells on isotropic and anisotropic topographies of silica thin films produced via microstamping (2010) Microsc Microanal, 16, pp. 670-676; 
Brett, P.M., Harle, J., Salih, V., Mihoc, R., Olsen, I., Jones, F.H., Tonetti, M., Roughness response genes in osteoblasts (2004) Bone, 35 (1), pp. 124-133. , DOI 10.1016/j.bone.2004.03.009, PII S8756328204001140; 
Klinge, B., Hultin, M., Berglundh, T., Peri-implantitis (2005) Dental Clinics of North America, 49 (3 SPEC. ISS.), pp. 661-676. , DOI 10.1016/j.cden.2005.03.007, PII S0011853205000236; 
Leonhardt, A., Renvert, S., Dahlen, G., Microbial findings at failing implants (1999) Clin Oral Implants Res, 10, pp. 339-345; 
Zinelis, S., Thomas, A., Syres, K., Silikas, N., Eliades, G., Surface characterization of zirconia dental implants (2010) Dent Mater, 26, pp. 295-305; 
Marzolin, C., Smith, S.P., Prentiss, M., Whitesides, G.M., Fabrication of glass microstructures by micro-molding of sol-gel precursors (1998) Advanced Materials, 10 (8), pp. 571-574; 
Santucci, S., Di Nardo, S., Lozzi, L., Passacantando, M., Picozzi, P., XPS analysis on SiO 2 sol-gel thin films (1995) J Electron Spectrosc Relat Phenom, 76, pp. 623-628; 
Braet, F., De Zanger, R., Wisse, E., Drying cells for SEM, AFM and TEM by hexamethyldisilazane: A study on hepatic endothelial cells (1997) Journal of Microscopy, 186 (1), pp. 84-87
},
	month = {2011///},
	pages = {581 - 589},
	abstract = {Titanium implants are the gold standard in dentistry; however, problems such as gingival tarnishing and peri-implantitis have been reported. For zirconia to become a competitive alternative dental implant material, surface modification techniques that induce guided tissue growth must be developed. Objectives: To develop alternative surface modification techniques to promote guided tissue regeneration on zirconia materials, for applications in dental implantology. Methods: A methodology that combined soft lithography and sol-gel chemistry was used to obtain isotropic micropatterned silica coatings on yttria-stabilized zirconia substrates. The materials were characterized via chemical, structural, surface morphology approaches. In vitro biological behavior was evaluated in terms of early adhesion and viability/metabolic activity of human osteoblast-like cells. Statistical analysis was conducted using one-way ANOVA/Tukey HSD post hoc test. Results: Isotropic micropatterned silica coatings on yttria-stabilized zirconia substrates were obtained using a combined approach based on sol-gel technology and soft lithography. Micropatterned silica surfaces exhibited a biocompatible behavior, and modulated cell responses (i.e. inducing early alignment of osteoblast-like cells). After 7 d of culture, the cells fully covered the top surfaces of pillar microstructured silica films. Significance: The micropatterned silica films on zirconia showed a biocompatible response, and were capable of inducing guided osteoblastic cell adhesion, spreading and propagation. The results herein presented suggest that surface-modified ceramic implants via soft lithography and sol-gel chemistry could potentially be used to guide periodontal tissue regeneration, thus promoting tight tissue apposition, and avoiding gingival retraction and peri-implantitis. {\textcopyright} 2011 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.},
	keywords = {Adhesion, article, Biological behavior, biomaterial, biomedical and dental materials, cell adhesion, Cell culture, Cell Line, cell proliferation, Cell response, cell survival, Ceramic implants, ceramics, chemistry, Coated Materials, Biocompatible, Coatings, confocal microscopy, crystallography, Dental implant materials, Dental implantology, Dental Implants, Dental Materials, Dental prostheses, diagnostic agent, dyes, reagents, indicators, markers and buffers, Gels, Gold standards, Growth kinetics, Guided tissue regeneration, Guided Tissue Regeneration, Periodontal, heat, Hot Temperature, human, Human osteoblast-like cells, Humans, In-vitro, Indicators and Reagents, Lithography, materials testing, Micropatterned, Microscopy, Confocal, Microscopy, Electron, Scanning, osteoblast, Osteoblast-like cells, Osteoblastic cell adhesion, Osteoblasts, oxazine derivative, Oxazines, Peri-implantitis, Periodontal tissue, periodontics, phase transition, physiology, Raman spectrometry, resazurin, Scanning electron microscopy, Silica, Silica coatings, Silica film, Silica surface, silicon dioxide, Soft lithography, Sol-gel, Sol-gel chemistry, Sol-gel process, Sol-gel technology, Sol-gels, Sols, Spectrum Analysis, Raman, Stabilized zirconia, Statistical analysis, Surface modification techniques, Surface morphology, Surface Properties, surface property, Surface-modified, Surfaces, time, Time Factors, Tissue, Tissue growth, Titanium, Titanium implants, tooth implantation, Top surface, X ray diffraction, X-Ray Diffraction, xanthene derivative, Xanthenes, yttria stabilized tetragonal zirconia, Yttria stabilized zirconia, yttrium, Yttrium alloys, Zirconia, Zirconia materials, zirconium},
	isbn = {01095641 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79955638770\&partnerID=40\&md5=31e0130d40aa381f08a781115b43fe2e},
	author = {Pelaez-Vargas, A. and Gallego-Perez, D. and Magallanes-Perdomo, M. and Fernandes, M.H. and Hansford, D.J. and De Aza, A.H. and Pena, P. and Monteiro, F.J.}
}
@article {2281,
	title = {Adhesion of human leukocytes on mixtures of hydroxyl- and methyl-terminated self-assembled monolayers: Effect of blood protein adsorption},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {93},
	year = {2010},
	note = {Cited By (since 1996): 4Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32480PubMed ID: 19484768Language of Original Document: EnglishCorrespondence Address: Barbosa, J. N.; Divis{\~a}o de Biomateriais, INEB-Instituto de Engenharia Biom{\'e}dica, Universidade do Porto, Porto 4150-180, Portugal; email: judite@ineb.up.ptChemicals/CAS: Blood Proteins; Gold, 7440-57-5; Hydroxyl Radical, 3352-57-6References: Brodbeck, W.G., Nakayama, Y., Matsuda, T., Colton, E., Ziats, N.P., Anderson, J.M., Biomaterial surface chemistry dictates adherent monocyte/macrophage cytokine expression in vitro (2002) Cytokine, 18, pp. 311-319;Kao, W.Y.J., Evaluation of leukocyte adhesion on polyurethanes: The effects of shear stress and blood proteins (2000) Biomaterials, 21, pp. 2295-2303; 
Tegoulia, V.A., Cooper, S.L., Leukocyte adhesion on model surfaces under flow: Effects of surface chemistry, protein adsorption, and shear rate (2000) J Biomed Mater Res, 50, pp. 291-301; 
Nimeri, G., Ohman, L., Elwing, H., Wettero, J., Bengtsson, T., The influence of plasma proteins and platelets on oxygen radical production and F-actin distribution in neutrophils adhering to polymer surfaces (2002) Biomaterials, 23, pp. 1785-1795; 
Wettero, J., Bengtsson, T., Tengvall, P., C1q-independent activation of neutrophils by immunoglobulin M-coated surfaces (2001) J Biomed Mater Res, 57, pp. 550-558; 
Yung, L.Y.L., Colman, R.W., Cooper, S.L., Neutrophil adhesion on polyurethanes preadsorbed with high molecular weight kininogen (1999) Blood, 94, pp. 2716-2724; 
Eriksson, C., Nygren, H., Polymorphonuclear leukocytes in coagulating whole blood recognize hydrophilic and hydrophobic titanium surfaces by different adhesion receptors and show different patterns of receptor expression (2001) J Lab Clin Med, 137, pp. 296-302; 
Tang, L.P., Eaton, J.W., Mechanism of acute inflammatory response to biomaterials (1994) Cell Mater, 4, pp. 429-436; 
Tang, L.P., Eaton, J.W., Inflammatory responses to biomaterials (1995) Am J Clin Pathol, 103, pp. 466-471; 
Jenney, C.R., Anderson, J.M., Adsorbed serum proteins responsible for surface dependent human macrophage behavior (1999) J Biomed Mater Res, 49, pp. 435-447; 
Collier, T.O., Anderson, J.M., Protein and surface effects on monocyte and macrophage adhesion, maturation, and survival (2002) J Biomed Mater Res, 60, pp. 487-496; 
Yun, J.K., Defife, K., Colton, E., Stack, S., Azeez, A., Cahalan, L., Verhoeven, M., Anderson, J.M., Human monocytemacrophage adhesion and cytokine production on surface-modified poly(tetrafluoroethylene hexafluoropropylene) polymers with and without protein preadsorption (1995) J Biomed Mater Res, 29, pp. 257-268; 
Ulman, A., Formation and structure of self-assembled monolayers (1996) Chem Rev, 96, pp. 1533-1554; 
Mrksich, M., Tailored substrates for studies of attached cell culture (1998) Cell Mol Life Sci, 54, pp. 653-662; 
Mrksich, M., Model substrates for studying and controlling cell adhesion (2000) Abstr Pap Am Chem Soc, 219, pp. U572-U572; 
Rodrigues, S.N., Goncalves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers (2006) Biomaterials, 27, pp. 5357-5367; 
Martins, M.C.L., Ratner, B.D., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methylterminated alkanethiols self-assembled monolavers (2003) J Biomed Mater Res, 67 A, pp. 158-171. , Part A; 
Anderson, J.M., Biological responses to materials (2001) Annu Rev Mater Res, 31, pp. 81-110; 
Tang, L.P., Liu, L., Elwing, H.B., Complement activation and inflammation triggered by model biomaterial surfaces (1998) J Biomed Mater Res, 41, pp. 333-340; 
Bergman, A.J., Zygourakis, K., Migration of lymphocytes on fibronectin-coated surfaces: Temporal evolution of migratory parameters (1999) Biomaterials, 20, pp. 2235-2244; 
Taborelli, M., Eng, L., Descouts, P., Ranieri, J.P., Bellamkonda, R., Aebischer, P., Bovine serum-albumin conformation on methyl and amine functionalized surfaces compared by scanning force microscopy (1995) J Biomed Mater Res, 29, pp. 707-714; 
Liu, L., Elwing, H., Complement activation on thiol-modi.ed gold surfaces (1996) J Biomed Mater Res, 30, pp. 535-541; 
Sperling, C., Schweiss, R.B., Streller, U., Werner, C., In vitro hemocompatibility of self-assembled monolayers displaying various functional groups (2005) Biomaterials, 26, pp. 6547-6557; 
Hirata, I., Hioko, Y., Toda, M., Kitazawa, T., Murakami, Y., Kitano, E., Kitamura, H., Iwata, H., Deposition of complement protein C3b on mixed self-assembled monolayers carrying surface hydroxyl and methyl groups studied by surface plasmon resonance (2003) J Biomed Mater Res, 66 A, pp. 669-676. , Part A; 
Kalltorp, M., Oblogina, S., Jacobsson, S., Karlsson, A., Tengvall, P., Thomsen, P., In vivo cell recruitment, cytokine release and chemiluminescence response at gold, and thiol functionalized surfaces (1999) Biomaterials, 20, pp. 2123-2137; 
Brash, J.L., Tenhove, P., Protein adsorption studies on standard polymeric materials (1993) J Biomater Sci Polym Ed, 4, pp. 591-599; 
Brash, J.L., Tenhove, P., Effect of plasma dilution on adsorption of fibrinogen to solid-surfaces (1984) Thromb Haemost, 51, pp. 326-330; 
Tang, L.P., Ugarova, T.P., Plow, E.F., Eaton, J.W., Molecular determinants of acute inflammatory responses to biomaterials (1996) J Clin Invest, 97, pp. 1329-1334; 
Thomsen, P., Gretzer, C., Macrophage interactions with modified material surfaces (2001) Curr Opin Solid State Mater Sci, 5, pp. 163-176; 
Werthen, M., Sellborn, A., Kalltorp, M., Elwing, H., Thomsen, P., In vitro study of monocyte viability during the initial adhesion to albumin- and fibrinogen-coated surfaces (2001) Biomaterials, 22, pp. 827-832; 
Wettero, J., Bengtsson, T., Tengvall, P., Complement activation on immunoglobulin G-coated hydrophobic surfaces enhances the release of oxygen radicals from neutrophils through an actin-dependent mechanism (2000) J Biomed Mater Res, 51, pp. 742-751; 
Jenney, C.R., Anderson, J.M., Adsorbed IgG: A potent adhesive substrate for human macrophages (2000) J Biomed Mater Res, 50, pp. 281-290; 
Lindblad, M., Lestelius, M., Johansson, A., Tengvall, P., Thomsen, P., Cell and soft tissue interactions with methyl- and hydroxyl-terminated alkane thiols on gold surfaces (1997) Biomaterials, 18, pp. 1059-1068; 
Roach, P., Farrar, D., Perry, C.C., Interpretation of protein adsorption: Surface-induced conformational changes (2005) J Am Chem Soc, 127, pp. 8168-8173; 
Barbosa, J.N., Barbosa, M.A., Aguas, A.P., Adhesion of human leukocytes to biomaterials: An in vitro study using alkanethiolate monolayers with different chemically functionalized surfaces (2003) J Biomed Mater Res, 65 A, pp. 429-434. , Part A; 
Barbosa, J.N., Barbosa, M.A., Aguas, A.P., Inflammatory responses and cell adhesion to self-assembled monolayers of alkanethiolates on gold (2004) Biomaterials, 25, pp. 2557-2563; 
Barbosa, J.N., Barbosa, M.A., Aguas, A.P., Inflammatory cell recruitment and adhesion to methyl-terminated selfassembled monolayers: Effect of implantation time (2005) Microsc Res Tech, 66, pp. 37-42; 
Barbosa, J.N., Madureira, P., Barbosa, M.A., Aguas, A.P., The attraction of Mac-1(+) phagocytes during acute inflammation by methyl-coated self-assembled monolayers (2005) Biomaterials, 26, pp. 3021-3027
},
	month = {2010///},
	pages = {12 - 19},
	abstract = {The adhesion of human leukocytes to nanostructured surfaces with different chemical properties and the effect of protein adsorption were investigated. Selfassembled monolayers (SAMs) prepared with mixtures of methyl- and hydroxyl-terminated alkanethiols in different percentages on gold were used. The surfaces were preimmersed in distinct protein solutions (human serum albumin, human fibrinogen, and autologous plasma). Adherent leukocytes were analyzed both by light and SEM. SAMs submitted to pre-immersion in plasma presented higher numbers of adherent leukocytes in the pure OH-terminated SAM, whereas methyl-terminated surfaces accounted for the lowest number of adherent cells. We observed a general increase in the number of adherent human leukocytes as the percentage of OH groups on the surface of the SAMs increased for all the pre-immersion conditions investigated. The number of adherent human leukocytes is highly influenced by the pre-immersion conditions used, and this observation is particularly relevant in the case of the methyl-terminated SAMs. The results obtained demonstrate that surface chemistry has a major influence in leukocyte adhesion to biomaterials, and that pre-immersion in protein solutions has a determinant effect in leukocyte adhesion. {\textcopyright} 2009 Wiley Periodicals, Inc.},
	keywords = {Adherent cells, Adhesion, Adsorption, Alkanethiols, article, Biological materials, biomaterial, Blood protein adsorption, Blood Proteins, cell adhesion, Cell Count, Chemical properties, gold, human, human cell, Human leukocytes, Human serum albumins, Humans, hydroxyl group, Hydroxyl Radical, Hydroxyl-terminated, immersion, Inflammatory response, leukocyte adherence, Leukocyte adhesion, Leukocytes, Nanostructured materials, Nanostructured surface, Nanostructured surfaces, OH group, Protein adsorption, protein blood level, Protein solution, Proteins, Sams, Scanning electron microscopy, Self assembled monolayers, Self-assembled monolayers, SEM, Surface chemistry, Surface Properties},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77649225392\&partnerID=40\&md5=0b35403e1b98581c557435b6cf7a8286},
	author = {Barbosa, J.N. and Martins, M.C.L. and Freitas, S.C. and Gon{\c c}alves, I.C. and {\'A}guas, A.P. and Barbosa, M.A.}
}
@article {2287,
	title = {Anti-adhesion and antiproliferative cellulose triacetate membrane for prevention of biomaterial-centred infections associated with Staphylococcus epidermidis},
	journal = {International Journal of Antimicrobial AgentsInt. J. Antimicrob. Agents},
	volume = {35},
	year = {2010},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: IAAGEdoi: 10.1016/j.ijantimicag.2009.09.017PubMed ID: 19942411Language of Original Document: EnglishCorrespondence Address: Extremina, C.I.; REQUIMTE, Faculdade de Farm{\'a}cia, Universidade do Porto, Rua An{\'\i}bal Cunha 164, 4050-047 Porto, Portugal; email: extremina@gmail.comChemicals/CAS: cellulose triacetate, 9012-09-3; cilastatin plus imipenem, 92309-29-0; Anti-Bacterial Agents; Biocompatible Materials; Cellulose, 9004-34-6; Imipenem, 74431-23-5; cellulose triacetate, 9012-09-3Tradenames: tienam, Merck Sharp and Dohme, GermanyManufacturers: Eastman Kodak, Netherlands; Merck Sharp and Dohme, GermanyReferences: Darouiche, R.O., Treatment of infections associated with surgical implants (2004) N Engl J Med, 350, pp. 1422-1429;Fonseca, A.P., Extremina, C., Fonseca, A.F., Sousa, J.C., Effect of subinhibitory concentration of piperacillin/tazobactam on Pseudomonas aeruginosa (2004) J Med Microbiol, 53, pp. 903-910; 
Donelli, G., Francolini, I., Piozzi, A., Di Rosa, R., Marconi, W., New polymer-antibiotic systems to inhibit bacterial biofilm formation: a suitable approach to prevent central venous catheter-associated infections (2002) J Chemother, 14, pp. 501-507; 
Akiyama, H., Okamoto, S., Prophylaxis of indwelling urethral catheter infection: clinical experience with a modified Foley catheter and drainage system (1979) J Urol, 121, pp. 40-42; 
Granja, P.L., Baquey, C., Pouys{\'e}gu, L., Bareille, R., De J{\'e}so, B., Barbosa, M.A., Cellulose-based biomaterials for bone regeneration: hemi-synthesis and characterisation (1996) Proceedings of polymers in medicine and surgery-PIMS{\textquoteright}96, pp. 73-80. , Glasgow, UK; 
Elam, J.H., Karlsson, C.H., Nygren, H., Pre-adsorption of cellulose ether onto polymer surfaces: adsorption of adhesins and platelet activation (1992) Biomaterials, 14, pp. 233-237; 
Fonseca, A.P., Granja, P.L., Nogueira, J.A., Oliveira, D.R., Barbosa, M.A., Staphylococcus epidermidis RP62A adhesion to chemically modified cellulose derivatives (2001) J Mater Sci Mater Med, 12, pp. 543-548; 
Performance standards for antimicrobial susceptibility testing (2006) Sixteenth informational supplement. Document M100-S16. Wayne, PA: CLSI, , Clinical and Laboratory Standards Institute; 
Vacheethasanee, K., Temenoff, J.S., Higashi, J.M., Gary, A., Anderson, J.M., Bayston, R., Bacterial surface properties of clinically isolated Staphylococcus epidermidis strains determine adhesion on polyethylene (1998) J Biomed Mater Res, 42, pp. 425-432; 
Bhat, N.V., Wavhal, D.S., Preparation of cellulose triacetate pervaporation membrane by ammonia plasma treatment (2000) J Appl Polym Sci, 76, pp. 258-265; 
Amaral, I.F., Granja, P.L., Melo Luis, V., Saramago, B., Barbosa, M.A., Functionalization of chitosan membranes through phosphorylation: atomic force microscopy, wettability, and cytotoxicity studies (2006) J Appl Polym Sci, 102, pp. 276-284; 
Thomas, M.J.K., Ando, D.J., (1996) Ultraviolet and visible spectroscopy: analytical chemistry by open learning, , Wiley, New York, NY; 
(1992) High resolution XPS of organic polymers: the Scienta ESCA300 database, , Beamson G., and Briggs D. (Eds), John Wiley \& Sons Ltd, Chichester, UK; 
Yigong Ge, Wikler, M.A., Sahm, D.F., Blosser-Middleton, R.S., Karlowsky, J.A., In vitro antimicrobial activity of doripenem, a new carbapenem (2004) Antimicrob Agents Chemother, 48, pp. 1384-1396; 
Mart{\'\i}nez-Mart{\'\i}nez, L., Pascual, A., Giglio, M.I., Perea, E.J., Effect of subinhibitory concentrations of β-lactams on the production of slime, surface hydrophobicity and adhesion of Staphylococcus epidermidis [in Spanish] (1991) Enferm Infecc Microbiol Clin, 9, pp. 543-546
},
	month = {2010///},
	pages = {164 - 168},
	abstract = {The initial step in preventing biomaterial-associated infections consists of preventing bacterial adhesion to the device surface. One possible approach is the design of antibiotic-releasing biomaterials. Cellulose triacetate (CTA) membranes with the antibiotic imipenem (IPM) entrapped (CTA-IPM) were prepared. The material was characterised in terms of surface morphology by scanning electron microscopy, surface free energy of interaction and X-ray photoelectron spectroscopy (XPS). Antibiotic release studies were also performed. In vitro adhesion of Staphylococcus epidermidis RP62A to CTA-IPM was investigated using a modified microtitre plate assay, and the antibacterial activity of the CTA-IPM membrane was assessed by a modified Kirby-Bauer test, which showed effective entrapment of the antibiotic as confirmed by XPS and hydrophilicity assays. Release studies showed that this drug-polymer conjugate serves as an adequate reservoir for sustained release of IPM over a period of 71 h at an effective bacteriostatic concentration. Moreover, bacterial adhesion tests showed a statistically significant decrease in the adhesion of S. epidermidis RP62A to CTA-IPM compared with its adhesion to CTA alone. The present innovative approach is capable of providing a membrane with anti-adhesive and antiproliferative properties, thus encouraging in vivo studies to provide a better simulation of the clinical situation. {\textcopyright} 2009 Elsevier B.V. and the International Society of Chemotherapy.},
	keywords = {Adhesion, Anti-Bacterial Agents, antibacterial activity, Antimicrobial agents, article, Bacterial adhesion, bacterial growth, bacteriostasis, bacterium adherence, Biocompatible Materials, biomaterial, Cellulose, Cellulose triacetate, cilastatin plus imipenem, controlled study, device infection, drug screening, Equipment and Supplies, growth inhibition, Humans, hydrophilicity, Imipenem, in vitro study, infection prevention, Membranes, Microbial Sensitivity Tests, microtiter plate assay, nonhuman, priority journal, Prosthesis-Related Infections, Scanning electron microscopy, Staphylococcus epidermidis, surface property, sustained drug release, X ray photoelectron spectroscopy},
	isbn = {09248579 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-72649093658\&partnerID=40\&md5=3fbe3d7e15447e7ae40bb5fdd3f63b61},
	author = {Extremina, C.I. and Fonseca, A.F.d. and Granja, P.L. and Fonseca, A.P.}
}
@article {2254,
	title = {Protein matrices for improved wound healing: Elastase inhibition by a synthetic peptide model},
	journal = {BiomacromoleculesBiomacromolecules},
	volume = {11},
	year = {2010},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: BOMAFdoi: 10.1021/bm100537bPubMed ID: 20690591Language of Original Document: EnglishCorrespondence Address: Vasconcelos, A.; Universidade Do Minho, Departamento de Engenharia T{\^e}xtil, Campus de Azur{\'e}m, 4800-058 Guimar{\~a}es, Portugal; email: andreiav@det.uminho.ptChemicals/CAS: Bowman Birk inhibitor, 37330-34-0; Fibroins, 9007-76-5; Keratins, 68238-35-7; Pancreatic Elastase, 3.4.21.36; Peptide Fragments; Trypsin Inhibitor, Bowman-Birk Soybean; Trypsin Inhibitors; fibroin, silkwormReferences: Boateng, J.S., Matthews, K.H., Stevens, H.N.E., Eccleston, G.M., Wound healing dressings and drug delivery systems: A review (2008) J. Pharm. Sci., 97, pp. 2892-2923;Rothe, M., Falanga, V., Growth factors: Their biology and promise in dermatologic diseases and tissue repair (1989) AMA Arch. Dermatol., 125, pp. 1390-1398; 
Shakespeare, P., Burn wound healing and skin substitutes (2001) Burns, 27, pp. 517-522; 
Martin, C.R., Wound infection: A failure of wound healing caused by an imbalance of bacteria (1997) Surg. Clin. North Am., 77, pp. 637-650; 
Stadelmann, W.K., Digenis, A.G., Tobin, G.R., Physiology and healing dynamics of chronic cutaneous wounds (1998) Am. J. Surg., 176, pp. 26S-38S; 
Eaglstein Wh, F.V., Chronic wounds (1997) Surg. Clin. North Am., 77, pp. 689-700; 
Gray, D.W.R., The wound exudate continuum: An aid to wound assessment (2004) Wounds U.K.: Appl. Wound Manageme. Suppl., pp. 19-21; 
Chen, W.Y.J., Rogers, A.A., Lydon, M.J., Characterization of biologic properties of wound fluid collected during early stages of wound healing (1992) J. Invest. Dermatol., 99, pp. 559-564; 
Brooke, B., Edward, J.C., Caroline, A.O., Leukocyte proteinases in wound healing: Roles in physiologic and pathologic processes (1999) Wound Repair Regener., 7, pp. 410-422; 
Dorne, R.Y., Benedict, C.N., The proteolytic environment of chronic wounds (1999) Wound Repair Regener., 7, pp. 433-441; 
Naomi, J.T., Michael, C.S., Shawn, M., Neil, B., Jane, G., Frank, B., Gillian, M., Gregory, S., Analysis of the acute and chronic wound environments: The role of proteases and their inhibitors (1999) Wound Repair Regener., 7, pp. 442-452; 
Enoch, S., Leaper, D.J., Basic science of wound healing (2008) Surgery (Oxford), 26, pp. 31-37; 
Chufa, H., Margaret, A.H., George, W.C., Frank, A., Effects of chronic wound fluid on the bioactivity of platelet-derived growth factor in serum-free medium and its direct effect on fibroblast growth (1999) Wound Repair Regener., 7, pp. 97-105; 
Chen, P., Rose, J., Love, R., Wei, C.H., Wang, B.C., Reactive sites of an anticarcinogenic Bowman-Birk proteinase inhibitor are similar to other trypsin inhibitors (1992) J. Biol. Chem., 267, pp. 1990-1994; 
Werner, M.H., Wemmer, D.E., Three-dimensional structure of soybean trypsin/chymotrypsin Bowman-Birk inhibitor in solution (1992) Biochemistry, 31, pp. 999-1010; 
Guangda, L.I.N., Wolfram, B., Robert, H., Chengwu, C.H.I., Richard, A.E., The 0.25-nm X-ray structure of the Bowman-Birk-type inhibitor from mung bean in ternary complex with porcine trypsin (1993) Eur. J. Biochem., 212, pp. 549-555; 
Voss, R.-H., Ulrich, E., Lars-Oliver, E., Gabriele, W., Young-Mi, K., Peter, F., Crystal structure of the bifunctional soybean Bowman-Birk inhibitor at 0.28-nm resolution (1996) Eur. J. Biochem., 242, pp. 122-131; 
Wolfram, B., Robert, H., Natural protein proteinase inhibitors and their interaction with proteinases (1992) Eur. J. Biochem., 204, pp. 433-451; 
Laskowski, M., Qasim, M.A., What can the structures of enzyme-inhibitor complexes tell us about the structures of enzyme substrate complexes (2000) Biochim. Biophys. Acta, Protein Struct. Mol. Enzymol., 1477, pp. 324-337; 
McBride, J.D., Leatherbarrow, R.J., Synthetic peptide mimics of the Bowman-Birk inhibitor protein (2001) Curr. Med. Chem., 8, pp. 909-917; 
Jeffrey, D.M., Emma, M.W., Arnd, B.E.B., Agn{\`e}s, M.J., Robin, J.L., Peptide mimics of the Bowman-Birk inhibitor reactive site loop (2002) Pept. Sci., 66, pp. 79-92; 
Vasconcelos, A., Freddi, G., Cavaco-Paulo, A., Biodegradable materials based on silk fibroin and keratin (2008) Biomacromolecules, 9, pp. 1299-1305; 
Ritger, P.L., Peppas, N.A., A simple equation for description of solute release II. Fickian and anomalous release from swellable devices (1987) J. Controlled Release, 5, pp. 37-42; 
Tanaka, H., Shimazu, T., Sugimoto, H., Yoshioka, T., Sugimoto, T., A sensitive and specific assay for granulocyte elastase in inflammatory tissue fluid using l-pyroglutamyl-l-prolyl-l-valine-p-nitroanilide (1990) Clin. Chim. Acta, 187, pp. 173-180; 
Yamauchi, K., Maniwa, M., Mori, T., Cultivation of fibroblast cells on keratin-coated substrata (1998) J. Biomater. Sci., Polym. Ed., 9, pp. 259-270; 
Li, M., Ogiso, M., Minoura, N., Enzymatic degradation behavior of porous silk fibroin sheets (2003) Biomaterials, 24, pp. 357-365; 
Minoura, N., Aiba, S., Higuchi, M., Gotoh, Y., Tsukada, M., Imai, Y., (1995) Biochem. Biophys. Res. Commun., 208, p. 511; 
Yamauchi, K., Hojo, H., Yamamoto, Y., Tanabe, T., Enhanced cell adhesion on RGDS-carrying keratin film (2003) Mater. Sci. Eng., C, 23, pp. 467-472; 
Roh, D.-H., Kang, S.-Y., Kim, J.-Y., Kwon, Y.-B., Young Kweon, H., Lee, K.-G., Park, Y.-H., Lee, J.-H., Wound healing effect of silk fibroin/alginate-blended sponge in full thickness skin defect of rat (2006) J. Mater. Sci.: Mater. Med., 17, pp. 547-552; 
Lu, S., Wang, X., Lu, Q., Hu, X., Uppal, N., Omenetto, F.G., Kaplan, D.L., Stabilization of Enzymes in Silk Films (2009) Biomacromolecules, 10, pp. 1032-1042; 
Hofmann, S., Wong Po Foo, C.T., Rossetti, F., Textor, M., Vunjak-Novakovic, G., Kaplan, D.L., Merkle, H.P., Meinel, L., Silk fibroin as an organic polymer for controlled drug delivery (2006) J. Controlled Release, 111, pp. 219-227; 
Numata, K., Cebe, P., Kaplan, D.L., Mechanism of enzymatic degradation of beta-sheet crystals (2010) Biomaterials, 31, pp. 2926-2933; 
Shudong, W., Youzhu, Z., Guibo, Y., Hongwei, W., Zhihui, D., Electrospun polylactide/silk fibroin-gelatin composite tubular scaffolds for small-diameter tissue engineering blood vessels (2009) J. Appl. Polym. Sci., 113, pp. 2675-2682; 
Motta, A., Migliaresi, C., Faccioni, F., Torricelli, P., Fini, M., Giardino, R., Fibroin hydrogels for biomedical applications: Preparation, characterization and in vitro cell culture studies (2004) J. Biomater. Sci., Polym. Ed., 15, p. 851; 
Bondar, B., Fuchs, S., Motta, A., Migliaresi, C., Kirkpatrick, C.J., Functionality of endothelial cells on silk fibroin nets: Comparative study of micro-and nanometric fibre size (2008) Biomaterials, 29, pp. 561-572; 
Fuchs, S., Motta, A., Migliaresi, C., Kirkpatrick, C.J., Outgrowth endothelial cells isolated and expanded from human peripheral blood progenitor cells as a potential source of autologous cells for endothelialization of silk fibroin biomaterials (2006) Biomaterials, 27, pp. 5399-5408; 
Unger, R.E., Wolf, M., Peters, K., Motta, A., Migliaresi, C., James Kirkpatrick, C., Growth of human cells on a non-woven silk fibroin net: A potential for use in tissue engineering (2004) Biomaterials, 25, pp. 1069-1075; 
Huang, Y., Yu, H., Xiao, C., PH-sensitive cationic guar gum/poly(acrylic acid) polyelectrolyte hydrogels: Swelling and in vitro drug release (2007) Carbohydr. Polym., 69, pp. 774-783; 
Edwards, J.V., Alvin, F.B., Sarah, B., Abul, J.U., Kelman, C.I., Robert, F.D., Steven, J.M., Inhibition of elastase by a synthetic cotton-bound serine protease inhibitor: In vitro kinetics and inhibitor release (1999) Wound Repair Regener., 7, pp. 106-118; 
Schneider, A., Wang, X.Y., Kaplan, D.L., Garlick, J.A., Egles, C., Biofunctionalized electrospun silk mats as a topical bioactive dressing for accelerated wound healing (2009) Acta Biomater., 5, pp. 2570-2578; 
Gariani, T., McBride, J.D., Leatherbarrow, R.J., The role of the P2' position of Bowman-Birk proteinase inhibitor in the inhibition of trypsin: Studies on P2' variation in cyclic peptides encompassing the reactive site loop (1999) Biochim. Biophys. Acta, Protein Struct. Mol. Enzymol., 1431, pp. 232-237; 
Zablotna, E., Kazmierczak, K., Jaskiewicz, A., Stawikowski, M., Kupryszewski, G., Rolka, K., Chemical synthesis and kinetic study of the smallest naturally occurring trypsin inhibitor SFTI-1 isolated from sunflower seeds and its analogues (2002) Biochem. Biophys. Res. Commun., 292, pp. 855-859
},
	month = {2010///},
	pages = {2213 - 2220},
	abstract = {The unique properties of silk fibroin were combined with keratin to develop new wound-dressing materials. Silk fibroin/keratin (SF/K) films were prepared to reduce high levels of elastase found on chronic wounds. This improved biological function was achieved by the incorporation of a small peptide synthesized based on the reactive-site loop of the Bowman-Birk Inhibitor (BBI) protein. In vitro degradation and release were evaluated using porcine pancreatic elastase (PPE) solution as a model of wound exudate. It was found that biological degradation and release rate are highly dependent on film composition. Furthermore, the level of PPE activity can be tuned by changing the film composition, thus showing an innovative way of controlling the elastase-antielastase imbalance found on chronic wounds. {\textcopyright} 2010 American Chemical Society.},
	keywords = {animal cell, Animals, article, Atomic force microscopy, Biodegradation, Biological degradation, Biological functions, biomaterial, Bombyx, Bowman Birk inhibitor, Bowman-Birk inhibitors, cell proliferation, Chronic wounds, controlled study, Cytotoxicity test, Degradation, Elastase, elastase inhibitor, enzyme activity, Enzyme inhibition, Fibroins, Film composition, In-vitro, keratin, Keratins, mass spectrometry, materials testing, Mice, Microscopy, Atomic Force, NIH 3T3 Cells, nonhuman, pancreatic elastase, Peptide Fragments, Peptides, priority journal, protein binding, Protein matrices, Release rate, Silk, Silk fibroin, Spectrometry, Mass, Electrospray Ionization, Sus, Swine, Synthetic peptide, Trypsin Inhibitor, Bowman-Birk Soybean, Trypsin Inhibitors, wound fluid, Wound healing},
	isbn = {15257797 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77956552375\&partnerID=40\&md5=7d7dc9145b9655d9ba0461eb7a85d118},
	author = {Vasconcelos, A. and P{\^e}go, A.P. and Henriques, L. and Lamghari, M. and Cavaco-Paulo, A.}
}
@article {2291,
	title = {Induction of notch signaling by immobilization of jagged-1 on self-assembled monolayers},
	journal = {BiomaterialsBiomaterials},
	volume = {30},
	year = {2009},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2009.09.010PubMed ID: 19783294Language of Original Document: EnglishCorrespondence Address: Gon{\c c}alves, R.M.; INEB, Biomaterials Division, NEWTherapies Group, R. Campo Alegre, 823, 4150-180 Porto, Portugal; email: raquelg@ineb.up.ptChemicals/CAS: Calcium-Binding Proteins; Gold, 7440-57-5; Immunoglobulin G; Intercellular Signaling Peptides and Proteins; Membrane Proteins; Receptors, Fc; Receptors, Notch; Recombinant Fusion Proteins; Serrate proteins, 134324-36-0References: Artavanis-Tsakonas, S., Rand, M.D., Lake, R.J., Notch signaling: cell fate control and signal integration in development (1999) Science, 284, pp. 770-776;Leong, K., Karsan, A., Recent insights in the role of notch signaling in tumorigenesis (2006) Blood, 107 (6), pp. 2223-2233; 
Chiba, S., Concise review: notch signaling in stem cell systems (2006) Stem Cells, 24, pp. 2437-2447; 
Lowell, S., Benchoua, A., Heavey, B., Smith, A.G., Notch promotes neural lineage entry by pluripotent embryonic stem cells (2006) PLoS Biol, 4 (5); 
Hellstr{\"o}m, M.P.L., Hofmann, J.J., Wallgard, E., Coultas, L., Lindblom, P., Alva, J., Dll4 signalling through notch1 regulates formation of tip cells during angiogenesis (2007) Nature, 15 (445), pp. 776-780; 
Hofmeister, C., Zhang, J., Knight, K.L., Le, P., Stiff, P.J., Ex vivo expansion of umbilical cord blood stem cells for transplantation: growing knowledge from the hematopoietic niche (2007) Bone Marrow Transplant, 39 (1), pp. 11-23; 
Hilton, M., Tu, X., Wu, X., Bai, S., Zhao, H., Kobayashi, T., F. Notch signaling maintains bone marrow mesenchymal progenitors by suppressing osteoblast differentiation (2008) Nat Med, 14 (3), pp. 306-314; 
Stylianou, S., Clarke, R.B., Brennan, K., Aberrant activation of notch signaling in human breast cancer (2006) Cancer Res, 66 (3), pp. 1517-1525; 
Demarest, R., Ratti, F., Capobianco, A.J., It{\textquoteright}s T-ALL about notch (2008) Oncogene, 27 (38), pp. 5082-5091; 
Fre, S., Huyghe, M., Mourikis, P., Robine, S., Louvard, D., Artavanis-Tsakonas, S., Notch signals control the fate of immature progenitor cells in the intestine (2005) Nature, 16 (435), pp. 964-968; 
Fischer, A., Gessler, M., Delta-notch-and then? Protein interactions and proposed modes of repression by Hes and Hey bHLH factors (2007) Nucleic Acids Res, 35 (14), pp. 4583-4596; 
Zweidler-McKay, P.A., He, Y., Xu, L., Rodriguez, C.G., Karnell, F.G., Carpenter, A.C., Notch signaling is a potent inducer of growth arrest and apoptosis in a wide range of B-cell malignancies (2005) Blood, 106 (12), pp. 3898-3906; 
Beckstead, B., Santosa, D.M., Giachelli, C.M., Mimicking cell-cell interactions at the biomaterial-cell interface for control of stem cell differentiation (2006) J Biomed Mater Res, 79 A, pp. 94-103; 
Varnum-Finney, B., Wu, L., Yu, M., Brashem-Stein, C., Staats, S., Flowers, D., Immobilization of Notch ligand, Delta-1, is required for induction of notch signaling (2000) J Cell Sci, 113 (23), pp. 4313-4318; 
Delaney, C., Varnum-Finney, B., Aoyama, K., Brashem-Stein, C., Bernstein, I.D., Dose-dependent effects of the notch ligand delta1 on ex vivo differentiation and in vivo marrow repopulating ability of cord blood cells (2005) Blood, 106, pp. 2693-2699; 
Lefort, N., Bennea, C., Leli{\`e}vrea, J.D., Dorivala, C., Balboa, M., Sakanoc, S., Short exposure to notch ligand delta-4 is sufficient to induce T-cell differentiation program and to increase the T cell potential of primary human CD34+ cells (2006) Exp Hematol, 34, pp. 1720-1729; 
Taqvi, S., Dixit, L., Krishnendu, R., Biomaterial-based notch signaling for the differentiation of hematopoietic stem cells into T cells (2006) J Biomed Mater Res, 79 A, pp. 689-697; 
Bernstein, I., Delaney, C., Dallas, M., Heimfeld, S., Varnum-Finney, B., Notch signaling in hematopoiesis: implication for improving stem cell transplantation (2008) Biol Blood Marrow Transplant, 14, pp. 94-99; 
De Smedt, M., Hoebeke, I., Reyvoet, K., Leclercq, G., Plum, J., Different thresholds of~Notch signaling bias human precursor cells toward B-, NK-, monocytic/dendritic-, or T-cell lineage in thymus microenvironment (2005) Blood, 106, pp. 3498-3506; 
Mrksich, M., What can surface chemistry do for cell biology? (2002) Curr Opin Chem Biol, 6, pp. 794-797; 
France, R., Short, R.D., Dawsonb, R.A., MacNeilb, S., Attachment of human keratinocytes to plasma copolymers of acrylic acid/octa-1,7-diene and allyl amine/octa-1,7-diene (1998) J Mater Chem, 81 (1), pp. 37-42; 
Neves, H., Weerkamp, F., Gomes, A.C., Naber, B., Gameiro, P., Becker, J.L., Effects of delta1 and jagged1 on early human hematopoiesis: correlation with expression of notch signaling-related genes in CD34+ Cells (2006) Stem Cells, 24, pp. 1328-1337; 
Sokolova, V., Epple, M., Inorganic nanoparticles as carriers of nucleic acids into cells (2008) Angewandte Chemie, 47, pp. 1382-1395; 
Yao, J., Duan, L., Fan, M., Yuan, J., Wu, X., Notch1 induces cell cycle arrest and apoptosis in human cervical cancer cells: involvement of nuclear factor kappa B inhibition (2007) Int J Gynecol Cancer, 17, pp. 502-510; 
Chadwick, N., Nostro, M.C., Baron, M., Mottram, R., Brady, G., Buckle, A., Notch signaling induces apoptosis in primary human CD34+ hematopoietic progenitor cells (2007) Stem Cells, 25, pp. 203-210; 
Love, J., Estroff, L.A., Kriebel, J.K., Nuzzo, R.G., Whitesides, G.M., Self-assembled monolayers of thiolates on metals as a form of nanotechnology (2005) Chem Rev, 105, pp. 1103-1169; 
Gehanno, V., Freitas, P.P., Veloso, A., Ferreira, J., Almeida, B., Sousa, J.B., Ion beam deposition of Mn-Ir spin valves (1999) IEEE Trans Magn, 35, pp. 4361-4367; 
Martins, M.C.L., Ratner, B., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methyl- terminated self-assembled monolyers (2003) J Biomed Mater Res, 67 (A), pp. 158-171; 
Martins, M.C.L., Curtins, S.A., Freitas, S.C., Salgueiro, P., Ratner, B.D., Barbosa, M.A., Molecularly designed surfaces for blood deheparinization using an immobilized heparin-binding peptide (2009) J Biomed Mater Res A, 88 (1), pp. 162-173; 
Barrias, C.C., Martins, M.C.L., Bidarra, S., S{\'a} Miranda, M.C., Barbosa, M.A., Adsorption of a therapeutic enzyme to self-assembled monolayers: effect of the surface chemistry and solution pH on the amount and activity of adsorbed enzyme (2005) Biomaterials, 26, pp. 2695-2704; 
Iodine-125. A guide to radioiodination techniques. In: techniques I-Agtr, ed.: Little Chalfort: Amersham Life Science 1993Horbett, T., Techniques for protein adsorption studies (1986) Techniques of biocompatibility tests, pp. 183-214. , DF W. (Ed), CRC Press Inc, Harcover; 
Ohishi, K., Varnum-Finney, B., Bernstein, I.D., Delta-1 enhances marrow and thymus repopulating ability of human CD34+ CD38- cord blood cells (2002) J Clin Invest, 110, pp. 1165-1174; 
Li, L., Chen, S., Zheng, J., Ratner, B.D., Jiang, S., Protein adsorption on oligo(ethylene glycol)-terminated alkanethiolate self-assembled monolayers: the molecular basis for nonfouling behavior (2005) J Phys Chem B, 109 (7), pp. 2934-2941; 
Tohda, S., Murata-Ohsawa, M., Sakano, S., Nara, N., Notch ligands, Delta-1 and Delta-4 supress the self-renewal capacity and long-term growth of two myeloblastic leukemia cell lines (2003) Int J Oncol, 22 (5), pp. 1073-1079; 
Tohda, S.K.H., Murakami, N., Sakano, S., Nara, N., Diverse effects of the Notch ligands Jagged1 and Delta1 on the growth and differentiation of primary acute myeloblastic leukemia cells (2005) Exp Hematol, 33, pp. 558-563; 
Aoyama, K., Delaney, C., Varnun-Finney, B., Kohn, A.D., Moon, R.T., Bernstein, I.D., The interaction of the Wnt and notch pathways modulates natural killer versus T cell differentiation (2007) Stem Cells, 25, pp. 2488-2497; 
Beckstead B, Tung JC, Liang KJ, Tavakkol Z, Usui ML, Olerud JE, et~al. Methods to promote notch signaling at the biomaterial interface and evaluation in a rafted organ culture model. J Biomed Mater Res A, in press. [Epub ahead of print]Semler, E., Dasgupta, A., Moghe, P.V., Cytomimetic engineering of hepatocyte morphogenesis and function by substrate-based presentation of acellular E-cadherin (2005) Tissue Eng, 11 (5-6), pp. 734-750; 
Zhao, Z., Matsui, H., Accurate immobilization of antibody-functionalized peptide nanotubes on protein-patterned arrays by optimizing their ligand-receptor interactions (2007) Small, 3 (8), pp. 1390-1393; 
Longo, G., Szleifer, Ligand-receptor interactions in tethered polymer layers (2005) Langmuir, 21, pp. 11342-11351; 
Gon{\c c}alves, I.C., Martins, M.C.L., Barbosa, M.A., Ratner, B.D., Protein adsorption and clotting time of pHEMA hydrogels modified with C18 ligands to adsorb albumin selectively and reversibly (2009) Biomaterials, 30 (29), pp. 5541-5551
},
	month = {2009///},
	pages = {6879 - 6887},
	abstract = {Notch signaling is a key mechanism during mammal development and stem cell regulation. This study aims to target and control Notch signaling by ligands immobilization using self-assembled monolayers (SAMs) as model surfaces. Non-fouling substrates were prepared by immersion of gold substrates in (1-Mercapto-11-undecyl)tetra(ethylene glycol) thiol solutions. These surfaces were activated with N,N'-carbonyldiimidazole (CDI) at different concentrations (0, 0.03, 0.3, 3 and 30 mg/ml) and an anti-human IgG, Fc specific fragment antibody (Ab) was covalently bound to EG4-SAMs to guarantee the correct exposure of the Notch ligand Jagged-1/Fc chimera (Jag-1). The presence of Ab and Jag-1 was confirmed by radiolabeling, X-ray photoelectron spectroscopy (XPS), ellipsometry and ELISA. The biological activity of Jag-1-Ab-SAMs was assessed by real-time PCR for Hes-1 family gene expression, a Notch pathway target gene, in HL-60 cell line. Results have shown an increase of the amount of immobilized Ab with increasing surface activator concentrations. Jag-1 concentration also increases with Ab concentration. Interestingly, a higher Jagged-1 exposure and fold increase in Hes-1 expression were obtained for surfaces activated with the lowest concentration of CDI (0.03 mg/ml). These results illustrate the great importance of ligands orientation and exposure, when compared with density. This investigation brings new insights into Notch signaling mechanisms. In particular, Jag-1-Ab-SAMs have shown to be adequate model surfaces to study Notch pathway activation and may provide a basis to develop new interfaces in biomaterials to control Notch mechanism in different cell systems. {\textcopyright} 2009 Elsevier Ltd.},
	keywords = {Activator concentration, Adequate models, Adsorption, Animals, article, Bioactivity, Biological activities, Biological materials, biomaterial, Calcium-Binding Proteins, Carbonyldiimidazole, Cell culture, Cell Line, Cell membranes, cell strain HL 60, Cell system, Chemical activation, Concentration (process), Concentration of, controlled study, Covalently bound, Ethylene, Ethylene Glycol, gene expression, gold, Gold substrates, HL-60 cell line, human, human cell, Human IgG, Humans, Immunoassay, immunoglobulin Fc fragment, Immunoglobulin G, immunoglobulin G antibody, Intercellular Signaling Peptides and Proteins, Jagged1, Ligands, Mammalia, Membrane Proteins, Model surface, Molecular Structure, Nanostructured materials, Nanostructured surfaces, Nonfouling, Notch receptor, Notch signaling, Photoelectron spectroscopy, priority journal, Protein adsorption, protein immobilization, Radiolabeling, Real-time PCR, Receptors, Fc, Receptors, Notch, Recombinant Fusion Proteins, Sams, self assembled monolayer, Self assembled monolayers, Self-assembled monolayers, signal transduction, Signaling, stem cell, Surface Properties, Target genes, Tetra, transcription factor HES 1, X ray photoelectron spectroscopy},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-70350188310\&partnerID=40\&md5=6c55d29cf8ae55288f3e87880171ec70},
	author = {Gon{\c c}alves, R.M. and Martins, M.C.L. and Almeida-Porada, G. and Barbosa, M.A.}
}
@article {2357,
	title = {Bone ingrowth in macroporous Bonelike                    {\textregistered} for orthopaedic applications
},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {4},
	year = {2008},
	note = {Cited By (since 1996): 13Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2007.06.009PubMed ID: 17716960Language of Original Document: EnglishCorrespondence Address: Santos, J.D.; FEUP - Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; email: jdsantos@fe.up.ptChemicals/CAS: Bone SubstitutesTradenames: BonelikeReferences: Akamatsu, Y., Koshino, T., Saito, T., Wada, J., Changes in osteosclerosis of the osteoarthritic knee after high tibial osteotomy (1997) Clin Orthop, 334, pp. 207-214;Coventry, M.B., Ilstrup, D.M., Wallrichs, S.L., Proximal tibial osteotomy. A critical long-term study of eighty-seven cases (1993) J Bone Joint Surg, 75 -A, pp. 196-201; 
Hernigou, P., Medevielle, D., Debeyre, J., Goutallier, D., Proximal tibial osteotomy for osteoarthritis with varus deformity. A ten to thirteen-year follow-up study (1987) J Bone Joint Surg Am, 69, pp. 332-354; 
Hernigou, P., Ma, W., Open wedge tibial osteotomy with acrylic bone cement as bone substitute (2001) Knee, 8, pp. 103-110; 
Koshino, T., Murase, T., Saito, T., Medial opening-wedge high tibial osteotomy with use of porous hydroxyapatite to treat medial compartment osteoarthritis of the knee (2003) J Bone Joint Surg Am, 85, pp. 78-85; 
Simske SJ, Ayers RA, Bateman TA. Porous Materials for Bone Engineered. In: Materials Science Forum. Zurich: Trans Tech Publications; 1997. Vol. 250, pp. 151-182Hing, K.A., Bone repair in the twenty-first century: biology, chemistry or engineering? (2004) Philos Trans A Math Phys Eng Sci, 362 (1825), pp. 2821-2850; 
Hui, P.W., Leung, P.C., Sher, A., Fluid conductance of cancellous bone graft as a predictor for graft-host interface healing (1996) J Biomech, 29 (1), pp. 123-132; 
Ben-Nissan, B., Natural bioceramics: from coral to bone and beyond (2003) Curr Opin Solid State Mater Sci, 7 (4-5), p. 283; 
Shors, E.C., Holmes, R.E., Porous hydroxyapatite (1993) Introduction to Biomaterials, pp. 181-198. , Hench L.L., and Wilson J. (Eds), World Scientific, Singapore; 
Prado da Silva, M.H., Lemos, A.F., Gibson, I.R., Ferreira, J.M.F., Santos, J.D., Porous glass reinforced hydroxyapatite materials produced with different organic additives (2002) J Non-Cryst Solids, 304, pp. 286-292; 
Prado da Silva, M.H., Lemos, A.F., Ferreira, J.M.F., Lopes, M.A., Santos, J.D., Production of porous biomaterials based on glass reinforced hydroxyapatite composites (2002) Key Eng Mater, 230-232, pp. 483-486; 
Rocha, J., Lemos, A., Agathopoulos, S., Val{\'e}rio, P., Kannan, S., Oktar, F., Scaffolds for bone restoration from cuttlefish (2005) Bone, 37 (6), pp. 850-857; 
Sepulveda, P., Binner, J.G.P., Processing of cellular ceramics by foaming and in situ polymerization of organic monomers (1999) J Eur Ceram Soc, 19 (12), pp. 2059-2066; 
Ozgur Engin, N., Cuneyt Tas, A., Manufacture of macroporous calcium hydroxyapatite bioceramics (1999) J Eur Ceram Soc, 19 (13-14), pp. 2569-2572; 
Peng, H.X., Fan, Z., Evans, J.R.G., Busfield, J.J.C., Microstructure of ceramic foams (2000) J Eur Ceram Soc, 20 (7), pp. 807-813; 
Komlev Vladimir, S., Barinov Serguei, M., Koplik Elena, V., A method to fabricate porous spherical hydroxyapatite granules intended for time-controlled drug release (2002) Biomaterials, 23 (16), pp. 3449-3454; 
Milosevski, M., Bossert, J., Milosevski, D., Gruevska, N., Preparation and properties of dense and porous calcium phosphate (1999) Ceram Int, 25, pp. 693-696; 
Tadic, D., Beckmann, F., Schwarz, K., Epple, M., A novel method to produce hydroxyapatite objects with interconnecting porosity that avoids sintering (2004) Biomaterials, 25, pp. 3335-3340; 
Lemos, A.F., Santos, J.D., Ferreira, J.M.F., Influence of characteristics of the starting hydroxyapatite powders and of deagglomeration procedure, on rheological behaviour of the suspensions (2004) Mater Sci Forum, 455-456, pp. 361-365; 
Lemos, A.F., Ferreira, J.M.F., Porous bioactive calcium carbonate implants processed by starch consolidation (2000) J Mat Sci Eng C, C11, pp. 35-40; 
Lemos, A.F., Ferreira, J.M.F., Combining foaming and starch consolidation methods to develop macroporous HA implants (2004) Key Eng Mater, 254-256, pp. 1041-1044; 
Lemos, A.F., Santos, J.D., Ferreira, J.M.F., New method for the incorporation of soluble bioactive glasses to reinforce porous HA structures (2004) Key Eng Mater, 254-256, pp. 1033-1036; 
Gauthiera, O., Boulerb, J.M., Aguadoa, E., Piletb, P., Daculsi, G., Macroporous biphasic calcium phosphate ceramics: influence of macropore diameter and macroporosity percentage on bone ingrowth (1998) Biomaterials, 19, pp. 133-139; 
Hing, A.K., Best, S.M., Tanner, K.E., Bonfield, W., Revell, P.A., Mediation of bone ingrowth in porous hydroxyapatite bone graft substitutes (2004) J Biomed Mater Res, 68 A, pp. 187-200; 
Rubin, P.A., Popham, J.K., Bilyk, J.R., Shore, J.W., Comparison of fibrovascular ingrowth into hydroxyapatite and porous polyethylene orbital implants (1994) Ophthal Plast Reconstr Surg, 10 (2), pp. 96-103; 
Hing, K.A., Annaz, B., Saeed, S., Revell, P.A., Buckland, T., Microporosity enhances bioactivity of synthetic bone graft substitutes (2005) J Mater Sci: Mater Med, 16, pp. 467-475; 
Klawitter, J.J., Bagwell, J.G., Weinstein, A.M., Sauer, B.W., An evaluation of bone growth into porous high density polyethylene (1976) J Biomed Mater Res, 10 (2), pp. 311-323; 
Hing, K.A., Best, S.M., Tanner, K.E., Bonfield, W., Revell, P.A., Quantification of bone ingrowth within bone-derived porous hydroxyapatite implants of varying density (1999) J Mater Sci: Mater Med, 10, pp. 663-670; 
Hing, K.A., Best, S.M., Tanner, K.E., Bonfield, W., Revell, P.A., Biomechanical assessment of bone ingrowth in porous hydroxyapatite (1997) J Mater Sci: Mater Med, 8, pp. 731-736; 
Ayers, R.A., Wolford, L.M., Bateman, T.A., Ferguson, V.L., Simske, S.J., Quantification of bone ingrowth into porous block hydroxyapatite in humans (1999) J Biomed Mater Res, 47, pp. 54-59; 
Lu, J.X., Flautre, B., Anselme, K., Hardouin, P., Gallur, A., Descamps, M., Role of interconnections in porous bioceramics on bone recolonization in vitro and in vivo (1999) J Mater Sci: Mater Med, 10 (2), pp. 111-120; 
Eggli, P.S., Muller, W., Schenk, R.K., Porous hydroxyapatite and tricalcium phosphate cylinders with two different pore size ranges implanted in the cancellous bone of rabbits - A comparative histomorphometric and histologic study of bony ingrowth and implant substitution (1988) Clin Orthop Relat Res, 232, pp. 127-138; 
Kuhne, J.H., Bartl, R., Frisch, B., Hammer, C., Jansson, V., Zimmer, M., Bone formation in coralline hydroxyapatite. Effects of pore size studied in rabbits (1994) Acta Orthop Scand, 65 (3), pp. 246-252; 
Holmes, R., Mooney, V., Bucholz, R., Tencer, A., A coralline hydroxyapatite bone graft substitute. Preliminary report (1984) Clin Orthop Relat Res, 188, pp. 252-262; 
Lopes, M.A., Knowles, J.C., Santos, J.D., Structural insights of glass-reinforced hydroxyapatite composites by Rietveld refinement (2000) Biomaterials, 21 (18), pp. 1905-1910; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass-reinforced hydroxyapatite: A comprehensive study of the effect of glass composition on the crystallography of the composite (1998) J Biomed Mater Res, 39 (2), pp. 244-251; 
Santos JD, Hastings G, Knowles JC. Sintered hydroxyapatite compositions and method for the preparation thereof. Worldwide Application (PCT), Patent No. 1 189 851, 1999Lopes, M.A., Monteiro, F.J., Santos, J.D., Glass-reinforced hydroxyapatite composites: fracture toughness and hardness dependence on microstructural characteristics (1999) Biomaterials, 20 (21), pp. 2085-2090; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Glass-reinforced hydroxyapatite composites: Secondary phase proportions and densification effects on biaxial bending strength (1999) J Biomed Mater Res, 48 (5), pp. 734-740; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Serro, A.P., Saramago, B., Hydrophobicity, Surface tension and Zeta potential measurements of glass reinforced hydroxyapatite composites (1999) J Biomed Mater Res, 45, pp. 370-375; 
Lopes, M.A., Knowles, J.C., Santos, J.D., Monteiro, F.J., Olsen, I., Direct and Indirect effects of P 2O 5 - glass reinforced - hydroxyapatite composites and growth and function of osteoblast-like cells (2000) Biomaterials, 21, pp. 1165-1172; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Push-out testing and histological evaluation of glass reinforced hydroxyapatite composites implanted in the tibiae of rabbits (2001) J Biomed Mater Res, 54, pp. 463-469; 
Ferraz, M.P., Fernandes, M.H., Santos, J.D., Monteiro, F.J., HA and Double layer HA-P 2O 5/CaO Glass coatings: influence of chemical composition on human bone marrow cells osteoblastic behaviour (2001) J Mater Sci: Mater Med, 12, pp. 629-638; 
Gutierres, M., Hussain, N.S., Afonso, A., Almeida, L., Cabral, T., Lopes, M.A., Biological behaviour of Bonelike {\textregistered} graft implanted in the tibia of humans (2005) Key Eng Mater, 17, pp. 1041-1044; 
Duarte, F., Santos, J.D., Afonso, A., Medical applications of Bonelike {\textregistered} in maxillofacial surgery (2004) Adv Mater Forum, pp. 370-373; 
Lobato, J.V., Hussain, N.S., Botelho, C.M., Titanium dental implants coated with Bonelike {\textregistered}: clinical case report (2006) Thin Solid Films, 515, p. 279; 
Coventry, M.B., Osteotomy of the upper portion of the tibia for degenerative arthritis of the knee: a preliminary report (1965) J Bone Joint Surg Am, 47, pp. 984-990; 
Coventry, M.B., Upper tibia osteotomy for gonarthrosis. The evolution of the operation in the last 18 years and long term results (1979) Orthop Clin North Am, 10, pp. 191-210; 
Coventry, M.B., Current concepts review. Upper tibia osteotomy for osteoarthritis (1985) J Bone Joint Surg Am, 67 A, pp. 1136-1140
},
	month = {2008///},
	pages = {370 - 377},
	abstract = {The aim of this study was to evaluate the biological behaviour of porous scaffold structures of Bonelike                        {\textregistered} which is suitable for either direct clinical use or tissue engineering applications. Porous cylindrical specimens 8 {\texttimes} 10 mm were implanted in the lateral aspect of the tibia of 13 patients (mean age 54 years), during osteotomy surgery for the treatment of medial compartment osteoarthritis of the knee. Implanted cylinders were retrieved at the same time as the removal of the blade plates at 3, 6, 9 and 12 months. Scanning electron microscopy and histological evaluations were performed to observe the biological responses of human bone tissue to porous Bonelike
                        {\textregistered}. The penetration depth was determined for all implantation periods, and after 6 months it was already possible to see new bone in the centre of the implanted cylinders, which gives 100\% of penetration depth for all implantations periods except for 3 months when bone could only be seen in the peripherical region. Regarding the percentage of the area covered by new bone calculated from two-dimensional histological sections, values of 53 {\textpm} 15, 76 {\textpm} 12 and 88 {\textpm} 9\% were achieved for 6, 9 and 12 months, respectively. Due to its structural features porous Bonelike
                        {\textregistered} permitted effective vascularization and bone ingrowth, and therefore was fully osteointegrated as shown in the histological surveys. A slow biomaterial degradation with implantation time is envisaged since the material has displayed surface degradation. Bonelike
                        {\textregistered} scaffolds show potential for complete ingrowth of osseous tissue and restoration of vascularization throughout the defected site. {\textcopyright} 2007.
},
	keywords = {adult, aged, article, biomaterial, bone defect, Bone graft, bone growth, Bone Regeneration, Bone Substitutes, bone tissue, Bonelike, clinical article, controlled study, Female, histology, human, human tissue, Humans, implant, knee osteoarthritis, male, materials testing, Microscopy, Electron, Scanning, Middle Aged, molecular scaffold, Orthopaedic, orthopedics, Osseointegration, Osteoarthritis, osteotomy, porosity, Porous materials, priority journal, Prostheses and Implants, Scanning electron microscopy, tibia, Time Factors, Tissue engineering, tissue section, vascularization, {\textregistered}},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-38949142954\&partnerID=40\&md5=ab5a278b6d421b9e8e96f9397b7c54e1},
	author = {Gutierres, M. and Lopes, M.A. and Sooraj Hussain, N. and Lemos, A.F. and Ferreira, J.M.F. and Afonso, A. and Cabral, A.T. and Almeida, L. and Santos, J.D.}
}
@article {2342,
	title = {Comparative study of nanohydroxyapatite microspheres for medical applications},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {86},
	year = {2008},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31634PubMed ID: 17975824Language of Original Document: EnglishCorrespondence Address: Mateus, A. Y. P.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: yovana@ibmc.up.ptChemicals/CAS: alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; calcium hydroxide, 1305-62-0; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; calcium, 7440-70-2; dodecyl sulfate sodium, 151-21-3; neutral red, 553-24-2; phosphoric acid, 7664-38-2; Biocompatible Materials; Durapatite, 1306-06-5; PowdersReferences: Jarcho, M., Calcium phosphates ceramics as hard tissue prosthetics (1981) Clin Orthop Relat Res, 157, pp. 259-278;Bucholz, R., Carlton, A., Holmes, R., Hydroxyapatite and tricalcium phosphate bone graft substitutes (1987) Orthop Clin N Am, 18, pp. 323-334; 
Daculsi, G., Passuti, N., Martins, S., Deudon, C., LeGeros, R., Raher, S., Macroporous calcium phosphate ceramic for ling bone surgery in humans and dogs: Clinical and histological study (1990) J Biomed Mater Res, 24, pp. 379-396; 
Gauthier, O., Bouler, J., Weiss, P., Bosco, J., Daculsi, G., Aguado, E., Kinetic study of bone in growth and ceramic resorption associated with the implantation of different injectable calcium-phosphate bone substitutes (1999) J Biomed Mater Res, 47, pp. 28-35; 
Rey, C., Calcium phosphate biomaterials and bone mineral: Differences in composition, structures and properties (1990) Biomaterials, 11, pp. 13-15; 
Yuan, H., Kurashina, K., de Bruijn, J., Li, Y., de Groot, K., Zhang, X., A preliminary study on osteoinduction of two kinds of calcium phosphate ceramics (1999) Biomaterials, 20, pp. 1799-1806; 
Liou, S., Chen, S., Liu, D., Synthesis and characterization of needlelike apatitic nanocomposite with controlled aspect ratios (2003) Biomaterials, 24, pp. 3981-3988; 
Earl, J.S., Wood, D.J., Mile, S.J., Hydrothermal synthesis of hydroxyapatite (2006) J Phys Conf, 26, pp. 268-271; 
Han, J.K., Song, H.Y., Fumio, S., Lee, B.T., Synthesis of high purity nano-sized hydroxyapatite powder by microwave-hydrothermal method (2006) Mater Chem Phys, 99, pp. 235-239; 
Yoshimura, M., Sujaridworakun, P., Koh, F., Fujiwara, T., Pongkao, D., Ahniyaz, A., Hydrothermal conversion of calcite crystals to hydroxyapatite (2004) Mater Sci Eng C, 24, pp. 521-525; 
Bogdanoviciene, I., Beganskiene, A., Tonsuaadu, K., Glaser, J., Meyer, H.J., Kareiva, A., Calcium hydroxyapatite Ca 10(PO 4) 6 (OH) 2 ceramics prepared by aqueous sol-gel processing (2006) Mater Res Bull, 41, pp. 1754-1762; 
Liu, D.M., Troczynski, T., Tseng, W.J., Water-based sol-gel synthesis of hydroxyapatite: Process development (2001) Biomaterials, 22, pp. 1721-1730; 
Bezzi, G., Celotti, G., Landi, E., La Torretta, T.M.G., Sopyan, I., Tampieri, A., A novel sol-gel technique for hydroxyapatite preparation (2003) Mater Chem Phys, 78, pp. 816-824; 
An, G.H., Wang, H.J., Kim, B.H., Jeong, Y.G., Choa, Y.H., Fabrication and characterization of a hydroxyapatite nanopowder by ultrasonic spray pyrolysis with salt-assisted decomposition (2007) Mater Sci Eng A, pp. 449-451,821-824; 
Varma, H.K., Babu, S., Synthesis of calcium phosphate bioceramics by citrate gel pyrolisis method (2005) Ceram Int, 31, pp. 109-114; 
Jarudilokkula, S., Tanthapanichakoonb, W., Boonamnuayvittayaa, V., Synthesis of hydroxyapatite nanoparticles using an emulsion liquid membrane system Colloids Surf A, , Forthcoming; 
Bose, S., Saha, S.K., Synthesis and characterization of hydroxyapatite nanopowders by emulsion technique (2003) Chem Mater, 15, pp. 4464-4469; 
Nayar, S., Sihna, M.K., Basu, D., Sinha, A., Synthesis and sintering of biomimetic hydroxyapatite nanoparticles for biomedical applications (2006) J Mater Sci: Mater Med, 17, pp. 1063-1068; 
Tas, A.C., Synthesis of biomimetic Ca-hydroxyapatite powders at 37{\textdegree}C in synthetic body fluids (2000) Biomaterials, 21, pp. 1429-1438; 
Wanga, A., Yina, H., Liua, D., Wub, H., Wadac, Y., Rena, M., Xua, Y., Chenga, X., Effects of organic modifiers on the size-controlled synthesis of hydroxyapatite nanorods (2007) Appl Surf Sci, 253, pp. 3311-3316; 
Zhang, H.G., Zhu, Q., Surfactant-assisted preparation of fluoride-substituted hydroxyapatite nanorods (2005) Mater Lett, 59, pp. 3054-3058; 
Liu, Y., Houa, D., Wangb, G., A simple wet chemical synthesis and characterization of hydroxyapatite nanorods (2004) Mater Chem Phys, 86, pp. 69-73; 
Pang, Y.X., Bao, X., Influence of temperature, ripening time and calcination on the morphology and crystallinity of hydroxyapatite nanoparticles (2003) J Eur Ceram Soc, 23, pp. 1697-1704; 
Gitzhofer, B.E., Boulos, M.I., Morpholgical study of hydroxyapatite nanocrystal suspension (2000) J Mater Sci: Mater Med, 11, pp. 523-531; 
Barbosa, M., Granja, P., Barrias, C., Amaral, I., Polysaccharides as scaffolds for bone regeneration (2005) ITBM-RBM, 26, pp. 212-217; 
Hari, P., Chandy, T., Sharma, C., Chitosan/calcium-alginate beads for oral delivery of insulin (1996) J Appl Polym Sci, 59, pp. 1796-1801; 
Barrias, C.C., Ribeiro, C.C., Barbosa, M.A., Adhesion and proliferation of human osteoblastic cells seeded on injectable hydroxyapatite microspheres (2004) Key Eng Mater, pp. 254-262,877-880; 
Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., Preparation and characterisation of calcium-phosphate porous microspheres with a uniform size for biomedical applications (2006) J Mater Sci: Mater Med, 17, pp. 455-463; 
Bowersock, T., Martins, S., Vaccine delivery to animals (1999) Adv Drug Deliv Rev, 32, pp. 167-194; 
Kong, H., Smith, M., Mooney, D., Designing alginate hydrogels to maintain viability of immobilized cells (2003) Biomaterials, 24, pp. 4023-4029; 
Legallais, C., David, B., Dor{\'e}, E., Bioartificial livers (BAL): Current technological aspects and future developments (2001) J Membr Sci, 181, pp. 81-95; 
Liu, X.D., Yu, W.Y., Zhang, Y., Xue, W.M., Yu, W.T., Xiong, Y., Ma, X.J., Yuan, Q., Characterization of structure and diffusion behaviour of Ca-alginate beads prepared with external or internal calcium sources (2002) J Microencapsul, 19 (6 -1), pp. 775-782; 
Taqieddin, E., Amiji, M., Enzyme immobilization in novel alginate-chitosan coreshell microcapsules (2004) Biomaterials, 25, pp. 1937-1945; 
Ribeiro, C., Barrias, C., Barbosa, M., Calcium phosphate-alginate microspheres as enzyme delivery matrices (2004) Biomaterials, 25, pp. 4363-4373; 
Kim, Y.J., Park, H.G., Yang, Y.L., Yoon, Y., Kim, S., Oh, E., Multifunctional drug delivery system using starch-alginate beads for controlled release (2005) Biol Pharm Bull, 28, pp. 394-397; 
Barrias, C., Ribeiro, C., Lamghari, M., Sa, C., Barbosa, M., Proliferation, activity and osteogenic differentiation of bone marrow stromal cells cultured on calcium titanium phosphate microspheres (2005) J Biomed Mater Res A, 72, pp. 57-66; 
Mateus, A.Y.P., Ferraz, M.P., Monteiro, F.J., Microspheres based on hydroxyapatite nanoparticles aggregates for bone regeneration (2007) Key Eng Mater, pp. 330-332,243-246; 
Mankani, M., Fowler, B., Kingman, A., Robey, P., In vivo bone formation by human bone marrow stromal cells: Effect of carrier particle size and shape (2001) Biotechonol Bioeng, 72, pp. 96-107; 
Higashi, T., Okamoto, H., Influence of particle size of calcium phosphate ceramics as a capping agent on the formation of a hard tissue barrier in amputated dental pulp (1996) J Endodoncy, 22, pp. 281-283; 
Guo, G., Xun, Y., Wnag, Z., Guo, H., Preparation of hydroxyapatite nanoparticles by reverse microemulsion (2005) Ceram Int, 331, pp. 869-872; 
Sun, X.M., Chen, X., Deng, Z.X., Li, Y.D., A CTAB-assited hydrothermal orientation growth of ZnO nanorods (2002) Mater Chem Phys, 78, pp. 99-104; 
Uota, M., Arakawa, H., Kitamura, N., Yoshimura, T., Tanaka, J., Kijima, T., Synthesis of high surface area hydroxyapatite nanoparticles by mixed surfactant-mediated approach (2005) Langmuir, 21, pp. 4724-4728; 
Balasundaram, G., Sato, M., Webster, T.J., Using hydroxyapatite nanoparticles and decreased crystallinity to promote osteoblast adhesion similar to functionalizing with RGD (2006) Biomaterials, 27, pp. 2798-2805; 
Wutticharoenmongkol, P., Sanchavanakit, N., Pavasant, P., Supaphol, P., Novel bone scaffolds or electrospun polycaprolactone fibers filled with nanoparticles (2006) J Nanosci Nanotechnol, 6, pp. 514-522; 
Gonzalez-McQuire, R., Green, D., Walsh, D., Hall, S., Chane-Ching, J., Oreffo, R.O.C., Mann, S., Fabrication of hydroxyapatite sponges by dextran sulphate/amino acid templating (2005) Biomaterials, 26, pp. 6652-6656; 
Sun, W.B., Wu, Y.F., Ding, Y., Chu, C.L., Liu, T.J., The expression and activity of alkaline phosphatase in human periodontal ligament cells with nanometer hydroxyapatite (2006) Zhonghua Kou Qiang Yi Xue Za Zhi, 41, pp. 348-349; 
Bowers, K., Keller, J.C., Randolph, B., Wick, D., Michaels, C., Optimization of surface micromorphology for enhanced osteoblast responses in vitro (1992) Int J Oral Maxillofac Impl, 7, pp. 302-310; 
Martin, J.Y., Schwartz, Z., Hummert, T.W., Schraub, D.M., Simpson, J., Lankford Jr, J., Dean, D.D., Boyan, B.D., Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63) (1995) J Biomed Mater Res, 29, pp. 389-401; 
Boyan, B.D., Batzer, R., Kieswetter, K., Liu, Y., Cochran, D.L., Szmuckler-Monclers, S., Dean, D.D., Schwartz, Z., Titanium surface roughness alters responsiveness of MG63 osteoblastic-like cells to 1α,25-(OH) 2D 3 (1998) J Biomed Mater Res, 39, pp. 77-85
},
	month = {2008///},
	pages = {483 - 493},
	abstract = {This study concerns the preparation, physical, and in vitro characterization of two different types of hydroxyapatite (HA) microspheres, which are intended to be used as drug-delivery systems and bone-regeneration matrices. Hydroxyapatite nanoparticles (HA-1 and HA-2) were prepared using the chemical precipitation synthesis with H                        3PO
                        4, Ca(OH) 
                        2, and a surfactant, SDS (sodium dodecyl sulfate), as starting reagents. The HA powders were dispersed in a sodium alginate solution, and spherical particles were obtained by droplet extrusion coupled with ionotropic gelation in the presence of Ca
                        2+. These were subsequently sintered to produce HA-1 and HA-2 microspheres with a uniform size and interconnected microporosity. Both powders and microspheres were characterized using FTIR and X-ray diffraction. Moreover, SEM and mercury intrusion porosimetry were used to analyze the microspheres, and TEM was used to analyze the powders. Results showed that pure HA and mixtures of HA/β-TCP in the nanometer range and needlelike shape were obtained for HA-1 and HA-2 powders, respectively. Neutral Red, scanning electron microscopy and confocal microscopy were used to evaluate the behavior of osteoblastic-like MG-63 cells cultured on HA microspheres surfaces for 7 days. Results showed that good adhesion and proliferation of osteoblasts on the HA microspheres surface. Cells built bridges between adjacent microspheres, forming microspheres-cells clusters in both types of materials. {\textcopyright} 2007 Wiley Periodicals, Inc.
},
	keywords = {alginic acid, analytic method, Apatite, article, Biocompatible Materials, Biological materials, biomaterial, Bone Regeneration, Calcium, calcium hydroxide, calcium phosphate, cell adhesion, Cell Line, cell proliferation, Chemical precipitation, Chemotherapy, Comparative studies, comparative study, controlled study, dodecyl sulfate sodium, Drug delivery, Drug delivery system (DDS), Drug Delivery Systems, Drug dosage, Durapatite, Gelation, human, human cell, Humans, hydroxyapatite, Hydroxyapatite (HAP), Hydroxylation, In vitro characterization, in vitro study, infrared spectroscopy, materials testing, Medical applications, Mercury intrusion porosimetry, microsphere, Microspheres, Nano-hydroxyapatite (n-HA), Nanohydroxyapatite, nanoparticle, nanoparticles, neutral red, osteoblast, Osteoblasts, Particle Size, phosphoric acid, porosity, powder, Powders, precipitation, Precipitation (chemical), reagent, Regeneration (renovating), Scanning electron microscopy, surfactant, synthesis, Synthesis (chemical), X ray diffraction},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-47049103112\&partnerID=40\&md5=138b3e2f3a97d8b398546d3f85567a0f},
	author = {Mateus, A.Y.P. and Barrias, C.C. and Ribeiro, C. and Ferraz, M.P. and Monteiro, F.J.}
}
@article {2381,
	title = {Attachment, spreading and short-term proliferation of human osteoblastic cells cultured on chitosan films with different degrees of acetylation},
	journal = {Journal of Biomaterials Science, Polymer EditionJ. Biomater. Sci. Polym. Ed.},
	volume = {18},
	year = {2007},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: JBSEEdoi: 10.1163/156856207780425068Language of Original Document: EnglishCorrespondence Address: Amaral, I.F.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: iamaral@ineb.up.ptChemicals/CAS: chitosan, 9012-76-4; Biocompatible Materials; Chitosan, 9012-76-4References: Vacanti, C.A., Bonassar, L.J., Vacanti, J.P., (2000) Principles of Tissue Engineering, p. 671. , R. P. Lanza, R. Langer and J. Vacanti Eds, p, Academic Press, San Diego, CA;Bruder, S.P., Caplan, A.I., (2000) Principles of Tissue Engineering, p. 683. , R. P. Lanza, R. Langer and J. Vacanti Eds, p, Academic Press, San Diego, CA; 
Burg, K.J.L., Porter, S., Kellam, J.F., (2000) Biomaterials, 21, p. 2347; 
Khor, E., Lim, L.Y., (2003) Biomaterials, 24, p. 2339; 
Nettles, D.L., Elder, S.H., Gilbert, J.A., (2002) Tissue Eng, 8, p. 1009; 
Lee, J.Y., Nam, S.H., Im, S.Y., Park, Y.J., Lee, Y.M., Seol, Y.J., Chung, C.P., Lee, S.J., (2002) J. Control. Rel, 78, p. 187; 
Muzzarelli, R.A.A., (1997) Cell. Mol. Life Sci, 53, p. 131; 
Madihally, S.V., Matthew, H.W.T., (1999) Biomaterials, 20, p. 1133; 
Chupa, J.M., Foster, A.M., Sumner, S.R., Madihally, S.V., Matthew, H.W.T., (2000) Biomaterials, 21, p. 2315; 
Lahiji, A., Sohrabi, A., Hungerford, D.S., Frondoza, C.G., (2000) J. Biomed. Mater. Res, 51, p. 586; 
Ho, M.H., Wang, D.M., Hsieh, H.J., Liu, H.C., Hsien, T.Y., Lai, J.Y., Hou, L.T., (2005) Biomaterials, 26, p. 3197; 
A. Domard, in: Advances in Chitin Science, II, A. Domard, G. A. F. Roberts and K. M. Varum (Eds), p. 410. Jacques Andr{\'e}, Lyon (1997)Tomihata, K., Ikada, Y., (1997) Biomaterials, 18, p. 567; 
Baxter, A., Dillon, M., Taylor, K.D.A., Roberts, G.A.F., (1992) Int. J. Biol. Macromol, 14, p. 166; 
Anselme, K., (2000) Biomaterials, 21, p. 667; 
Prasitsilp, M., Jenwithisuk, R., Kongsuwan, K., Damrongchai, N., Watts, P., (2000) J. Mater. Sci. Mater. Med, 11, p. 773; 
Chatelet, C., Damour, O., Domard, A., (2001) Biomaterials, 22, p. 261; 
Dhiman, H.K., Ray, A.R., Panda, A.K., (2004) Biomaterials, 25, p. 5147; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arguelles-Monal, W., Desbrieres, J., Rinaudo, M., (2001) Polymer, 42, p. 3569; 
Mima, S., Miya, M., Iwamoto, R., Yoshikawa, S., (1983) J. Appl. Polym. Sci, 28, p. 1909; 
Aiba, S., (1986) Int. J. Biol. Macromol, 8, p. 173; 
Terbojevich, M., Cosani, A., Muzzarelli, R.A.A., (1996) Carbohydr. Polym, 29, p. 63; 
(1987) Guideline on Validation of the Limulus Amebocyte Lysate Test as an End-product Endotoxin Test for Human and Animal Parental Drugs, Biological Products, and Medical Devices, , US Department of Health and Human Services, Public Health Service, Food and Drug Administration, US Department of Health and Human Services, Washington, DC; 
Nakagawa, Y., Murai, T., Hasegawa, C., Hirata, M., Tsuchiya, T., Yagami, T., Haishima, Y., (2003) J. Biomed. Mater. Res, 66 B, p. 347; 
Walker, S.L., Bhattacharjee, S., Hoek, E.M.V., Elimelech, M., (2002) Langmuir, 18, p. 2193; 
Clover, J., Gowen, M., (1994) Bone, 15, p. 585; 
Mosmann, T., (1983) J. Immunol. Methods, 65, p. 55; 
Domke, J., Dannohl, S., Parak, W.J., Muller, O., Aicher, W.K., Radmacher, M., (2000) Colloid Surfaces B: Biointerfaces, 19, p. 367; 
Sorlier, P., Denuziere, A., Viton, C., Domard, A., (2001) Biomacromolecules, 2, p. 765; 
Amaral, I.F., Lamghari, M., Sousa, S.R., Sampaio, P., Barbosa, M.A., (2005) J. Biomed. Mater. Res, 75 A, p. 387; 
Andrade, J.D., Smith, L.M., Gregonis, D.E., (1985) Surface and Interfacial Aspects of Biomedical Polymers, 1, p. 249. , J. D. Andrade Ed, p, Plenum Press, New York, NY; 
Dewez, J.L., Doren, A., Schneider, Y.J., Rouxhet, P.G., (1999) Biomaterials, 20, p. 547; 
Kikuchi, T., Yoshikai, Y., Miyoshi, J., Katsuki, M., Musikacharoen, T., Mitani, A., Tanaka, S., Matsuguchi, T., (2003) J. Dent. Res, 82, p. 546; 
Denuziere, A., Ferrier, D., Damour, O., Domard, A., (1998) Biomaterials, 19, p. 1275; 
Shirosaki, Y., Tsuru, K., Hayakawa, S., Osaka, A., Lopes, M.A., Santos, J.D., Fernandes, M.H., (2005) Biomaterials, 26, p. 485; 
Wang, J.H., Wei, C.W., Liu, H.C., Young, T.H., (2003) J. Biomed. Mater. Res, 64 A, p. 606; 
Michael, K.E., Vernekar, V.N., Keselowsky, B.G., Meredith, J.C., Latour, R.A., Garcia, A.J., (2003) Langmuir, 19, p. 8033; 
Keselowsky, B.G., Collard, D.M., Garcia, A.J., (2003) J. Biomed. Mater. Res, 66 A, p. 247; 
Cuy, J.L., Beckstead, B.L., Brown, C.D., Hoffman, A.S., Giachelli, C.M., (2003) J. Biomed. Mater. Res, 67 A, p. 538; 
McFarland, C.D., Mayer, S., Scotchford, C., Dalton, B.A., Steele, J.G., Downes, S., (1999) J. Biomed. Mater. Res, 44, p. 1; 
Cowles, E.A., Brailey, L.L., Gronowicz, G.A., (2000) J. Biomed. Mater. Res, 52, p. 725; 
Marco, R.A.W., Diaz-Montero, C.M., Wygant, J.N., Kleinerman, E.S., McIntyre, B.W., (2003) J. Cell. Biochem, 88, p. 1038; 
Martin, J.Y., Schwartz, Z., Hummert, T.W., Schraub, D.M., Simpson, J., Lankford, J., Dean, D.D., Boyan, B.D., (1995) J. Biomed. Mater. Res, 29, p. 389; 
Anselme, K., Bigerelle, M., Noel, B., Dufresne, E., Judas, D., Iost, A., Hardouin, P., (2000) J. Biomed. Mater. Res, 49, p. 155; 
Zinger, O., Anselme, K., Denzer, A., Habersetzer, P., Wieland, M., Jeanfils, J., Hardouin, P., Landolt, D., (2004) Biomaterials, 25, p. 2695
},
	month = {2007///},
	pages = {469 - 485},
	abstract = {Chitosan (Ch) is being actively investigated as a non-protein template for the growth of an increasing number of anchorage-dependent cells, including chondrocytes and bone cells. In the present work, Ch films with degrees of N-acetylation (DAs) in the range of 4 to 49\% were evaluated with respect to the attachment, spreading and short-term proliferation of osteoblasts, using human osteoblastic MG-63 cells. The films were characterized in terms of surface morphology and surface charge by atomic force microscopy and streaming potential measurements, respectively. Cell attachment was assessed after 3 and 24 h of cell culture. After 24 h of incubation, cell attachment was found to be dependent on the DA, lower DAs favouring cell adhesion. With time, cell spreading and cytoskeleton organization were only attained for DAs <= 13\%. Regarding cell proliferation, cells grown on films with the lowest DA (4\%) revealed a higher specific growth rate as compared to those grown on films with higher DAs. Films with a DA of 49\% failed to sustain cell proliferation. In addition, a longer lag-phase was observed on Ch, as compared to TCPS, in accordance to an observed delay of cell spreading. The present findings revealed that differences in the DA as small as 9\% may be critical in terms of the osteoblast response to two-dimensional Ch-based matrices. {\textcopyright} 2007 VSP.},
	keywords = {Acetylation, AFM, article, Atomic force microscopy, Biocompatible Materials, biofilm, Biofilms, biomaterial, bone cell, cartilage cell, cell adhesion, cell anchorage, Cell culture, cell growth, Cell Line, Tumor, cell proliferation, Cell Shape, cell spreading, cell survival, Chemical structure, chemistry, Chitin, chitosan, comparative study, controlled study, cytoskeleton, Degree of acetylation, drug effect, Growth kinetics, human, human cell, Humans, incubation time, metabolism, Microscopy, Atomic Force, osteoblast, Osteoblast-like cells, Osteoblasts, Pathology, priority journal, Surface charge, Surface Properties, surface property, Surface treatment, tumor cell line, Zeta potential},
	isbn = {09205063 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34347375992\&partnerID=40\&md5=437b9ed270e859425b76aa3655c611be},
	author = {Amaral, I.F. and Cordeiro, A.L. and Sampaio, P. and Barbosa, M.A.}
}
@article {2378,
	title = {Laser surface treatment of hydroxyapatite for enhanced tissue integration: Surface characterization and osteoblastic interaction studies},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {81},
	year = {2007},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31073PubMed ID: 17252543Language of Original Document: EnglishCorrespondence Address: Teixeira, S.; Laborat{\'o}rio de Biomateriais, INEB - Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre 823, 4150-180, Porto, Portugal; email: smsilva@ineb.up.ptChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; Ceramics; Durapatite, 1306-06-5Manufacturers: Plasma BiotalReferences: Teixeira S, Queiroz AC, Monteiro FJ, Ferraz MP, Vilar R, Eugenio S. Osteoblast proliferation and morphology analysis on laser modified hydroxyapatite surfaces: Preliminary results. Bioceramics 2006;18 (Parts 1, 2):309-311, 105-108Fernandez-Pradas, J.M., Cleries, L., Martinez, E., Sardin, G., Esteve, J., Morenza, J.L., Influence of thickness on the properties of hydroxyapatite coatings deposited by KrF laser ablation (2001) Biomaterials, 22, pp. 2171-2175;Fernandez-Pradas, J.M., Cleries, L., Serra, P., Sardin, G., Morenza, J.L., Evolution of the deposition rate during pulsed laser deposition of hydroxyapatite coatings and its relation with target morphology (2001) Appl Phys A: Mater Sci Process, 72, pp. 613-618; 
Foltyn, S.R., Dye, R., Ott, K.C., Peterson, E., Hubbard, K.M., Hutchinson, W., Muenchausen, R.E., Wu, X.D., Target modification in the excimer laser deposition of YBa 2Cu 3O 7-x thin films (1991) Appl Phys Lett, 59, pp. 594-596; 
Khor, K.A., Vreeling, A., Dong, Z.L., Cheang, P., Laser treatment of plasma sprayed HA coatings (1999) Mater Sci Eng A: Struct Mater Prop Microstruct Process, 266 (1-2), pp. 1-7; 
Ricci, J.L., Alexander, H., Laser microtexturing of implant surfaces for enhanced tissue integration (2001) Key Engineering Mater, pp. 198-199,179-202; 
Galli, C., Guizzardi, S., Passeri, G., Martini, D., Tinti, A., Mauro, G., Macaluso, G.M., Comparison of human mandibular osteoblasts grown on two commercially available titanium implant surfaces (2005) J Periodontol, 76, pp. 364-372; 
Oliveira, V., Conde, O., Vilar, R., UV laser micromachining of ceramic materials: Formation of columnar topographies (2001) Adv Eng Mater, 3 (1-2), pp. 75-81; 
Serra, P., FernandezPradas, J., Sardin, G., Morenza, J.L., Interaction effects of an excimer laser beam with hydroxyapatite targets (1997) Appl Surf Sci, 110, pp. 384-388; 
Serra, P., Morenza, J.L., Fluence dependence of hydroxyapatite laser ablation plumes (1998) Thin Solid Films, 335 (1-2), pp. 43-48; 
Duncan, A.C., Weisbuch, F., Rouais, F., Lazare, S., Baquey, C., Laser microfabricated model surfaces for controlled cell growth (2002) Biosens Bioelectron, 17, pp. 413-426; 
Walboomers, X.F., Croes, H.J.E., Ginsel, L.A., Jansen, J.A., Contact guidance of rat fibroblasts on various implant materials (1999) J Biomed Mater Res, 47, pp. 204-212; 
Carvalho, P., Manzanares, M.C., Franch, J., Belmonte, A.M., Lafuente, M.P., Serra, I., Fernandez-Pradas, J.M., Morenza, J.L., Osteointegration of calcium phosphate coatings obtained by pulsed laser deposition (2003) J Dent Res, 82, p. 573; 
Ahmad, M., McCarthy, M., Gronowicz, G., An in vitro model for mineralization of human osteoblast-like cells on implant materials (1999) Biomaterials, 20, pp. 211-220; 
Matsuura, T., Hosokawa, R., Okamoto, K., Kimoto, T., Akagawa, Y., Diverse mechanisms of osteoblast spreading on hydroxyapatite and titanium (2000) Biomaterials, 21, pp. 1121-1127; 
Torricelli, P., Fini, M., Giavaresi, G., Borsari, V., Carpi, A., Nicolini, A., Giardino, R., Comparative interspecies investigation on osteoblast cultures: Data on cell viability and synthetic activity (2003) Biomed Pharmacother, 57, pp. 57-62; 
Boyan, B.D., Lossdoerfer, S., Wang, L., Zhao, G., Lohmann, C.H., Cochran, D.L., Schwartz, Z., Osteoblasts generate an osteogenic microenvironment when grown on surfaces with rough microtopographies (2003) Eur Cell Mater, 6, pp. 22-27; 
Anselme, K., Bigerelle, M., Topography effects of pure titanium substrates on human osteoblast long-term adhesion (2005) Acta Biomater, 1, pp. 211-222; 
Anselme, K., Linez, P., Bigerelle, M., Le Maguer, D., Le Maguer, A., Hardouin, P., Hildebrand, H.F., Leroy, J.M., The relative influence of the topography and chemistry of TiAl 6V 4 surfaces on osteoblastic cell behaviour (2000) Biomaterials, 21, pp. 1567-1577; 
Anselme, K., Noel, B., Hardouin, P., Human osteoblast adhesion on titanium alloy, stainless steel, glass and plastic substrates with same surface topography (1999) J Mater Sci: Mater Med, 10, pp. 815-819; 
Cleries L, Fernandez-Pradas JM, Morenza JL. Bone growth on and resorption of calcium phosphate coatings obtained by pulsed laser deposition. J Biomed Mater Res 2000;49:43. Erratum 2001;57:473Deligianni, D.D., Katsala, N., Ladas, S., Sotiropoulou, D., Amedee, J., Missirlis, Y.F., Effect of surface roughness of the titanium alloy Ti-6Al-4V on human bone marrow cell response and on protein adsorption (2001) Biomaterials, 22, pp. 1241-1251; 
dos Santos, E.A., Farina, M., Soares, G.A., Specific proliferation rates of human osteoblasts on calcium phosphate surfaces with variable concentrations of α-TCP (2007) Mater Sci Eng, 27, pp. 61-66; 
Hao, L., Lawrence, J., Laser surface treatment of magnesia partially stabilized zirconia for enhanced human skin fibroblast cell response (2004) J Laser Appl, 16, pp. 55-64; 
Kieswetter, K., Schwartz, Z., Dean, D.D., Boyan, B.D., The role of implant surface characteristics in the healing of bone (1996) Crit Rev Oral Biol Med, 7, pp. 329-345; 
Salda{\~n}a, L., Gonz{\'n}lez-Carrasco, J.L., Rodriguez, M., Munuera, L., Vilaboa, N., Osteoblast response to plasma-spray porous Ti 6AL 4V coating on substrates of identical alloy (2006) J Biomed Mater Res A, 77, pp. 608-617; 
Lincks, J., Boyan, B.D., Blanchard, C.R., Lohmann, C.H., Liu, Y., Cochran, D.L., Dean, D.D., Schwartz, Z., Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition (1998) Biomaterials, 19, pp. 2219-2232; 
Martin, J.Y., Dean, D.D., Cochran, D.L., Simpson, J., Boyan, B.D., Schwartz, Z., Proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63) cultured on previously used titanium surfaces (1996) Clin Oral Implants Res, 7, pp. 27-37; 
Noth, U., Hendrich, C., Merklein, F., Altvater, T., Rader, C.P., Schutze, N., Eulert, J., Thull, R., Standardised testing of bone-implant surfaces using an osteoblast cell culture system. II. Titanium surfaces of varying degrees of roughness (1999) Biomedizinische Technik, 44 (1-2), pp. 6-11; 
Ponsonnet, L., Reybier, K., Jaffrezic, N., Comte, V., Lagneau, C., Lissac, M., Martelet, C., Relationship between surface properties (roughness, wettability) of titanium and titanium alloys and cell behaviour (2003) Mater Sci Eng C: Biomimetic Supramolecular Syst, 23, pp. 551-560; 
Ramires, P.A., Romito, A., Cosentino, F., Milella, E., The influence of titania/hydroxyapatite composite coatings on in vitro osteoblasts behaviour (2001) Biomaterials, 22, pp. 1467-1474; 
Redey, S.A., Nardin, M., Bernache-Assolant, D., Rey, C., Delannoy, P., Sedel, L., Marie, P.J., Behavior of human osteoblastic cells on stoichiometric hydroxyapatite and type A carbonate apatite: Role of surface energy (2000) J Biomed Mater Res, 50, pp. 353-364; 
Rosa, A.L., Beloti, M.M., van Noort, R., Osteoblastic differentiation of cultured rat bone marrow cells on hydroxyapatite with different surface topography (2003) Dent Mater, 19, pp. 768-772; 
Schmidt, C., Ignatius, A.A., Claes, L.E., Proliferation and differentiation parameters of human osteoblasts on titanium and steel surfaces (2001) J Biomed Mater Res, 54, pp. 209-215; 
Schwartz, Z., Kieswetter, K., Dean, D.D., Boyan, B.D., Underlying mechanisms at the bone-surface interface during regeneration (1997) J Periodontal Res, 32, pp. 166-171; 
Schwartz, Z., Martin, J.Y., Dean, D.D., Simpson, J., Cochran, D.L., Boyan, B.D., Effect of titanium surface roughness on chondrocyte proliferation, matrix production, and differentiation depends on the state of cell maturation (1996) J Biomed Mater Res, 30, pp. 145-155; 
Wang, J., de Boer, J., de Groot, K., Preparation and characterization of electrodeposited calcium phosphate/chitosan coating on Ti 6Al 4V plates (2004) J Dent Res, 83, pp. 296-301; 
Xavier, S.P., Carvalho, P.S.P., Beloti, M.M., Rosa, A.L., Response of rat bone marrow cells to commercially pure titanium submitted to different surface treatments (2003) J Dent, 31, pp. 173-180
},
	month = {2007///},
	pages = {920 - 929},
	abstract = {Biocompatibility has long been associated with surface microtopography, microtexture, and microchemistry. The surface topography eventually affects the nature and the intensity of the interactions that occur at biomaterial- biological interface (cell adhesion, mobility, spreading, and proliferation). Therefore, it is necessary to produce and work with controlled microtopographical surfaces that present reproducible microdomains of a dimension similar to that of the biological elements of interest (in this case, osteoblasts). There are a number of substrates that already have been studied in terms of surface topography; however, few studies are related to hydroxyapatite (HA) substrates. As it is well established, HA is a well-known ceramic that is extremely used in medical applications, namely implants and coatings. In this work, the surface topography of dense HA substrates was altered by using KFr excimer laser. The surface was characterized by atomic force microscopy and contact angle measurements, while the cell distribution and morphology was assessed by scanning electron microscopy and confocal laser scanning microscopy. Results revealed that the surface is characterized by a homogeneous columnar structure with high specific area. Moreover, cells were able to attach and spread on the surface of the samples, and gradually grow into nearly confluent monolayers. {\textcopyright} 2007 Wiley Periodicals, Inc.},
	keywords = {article, Atomic force microscopy, Biocompatibility, biomaterial, cell adhesion, Cell Communication, Cell Line, Tumor, cell motility, cell proliferation, cell spreading, cellular distribution, ceramic prosthesis, ceramics, chemistry, confocal laser microscopy, controlled study, Durapatite, Excimer laser, Excimer lasers, human, human cell, Humans, hydroxyapatite, laser, Lasers, material coating, Microchemistry, Microscopy, Atomic Force, Microscopy, Confocal, Microscopy, Electron, Scanning, Microtexture, monolayer culture, osteoblast, Osteoblasts, reproducibility, Scanning electron microscopy, Surface microtopography, Surface modification, Surface Properties, surface property, Surface topography, Surface treatment, Tissue engineering},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-34249742544\&partnerID=40\&md5=b6cb0aaa7b8e4a267b97d91d7171862f},
	author = {Teixeira, S. and Monteiro, F.J. and Ferraz, M.P. and Vilar, R. and Eug{\'e}nio, S.}
}
@article {2410,
	title = {Effect of surface treatments on anodic oxide film growth and electrochemical properties of tantalum used for biomedical applications},
	journal = {Journal of Biomaterials ApplicationsJ. Biomater. Appl.},
	volume = {21},
	year = {2006},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: JBAPEdoi: 10.1177/0885328206056378Language of Original Document: EnglishCorrespondence Address: Silva, R.A.; Instituto Superior de Engenharia do Porto, ISEP-CIEA, Rua Dr. A.B. de Almeida, 431, 4200-072 Porto, Portugal; email: ras@isep.ipp.ptChemicals/CAS: oxide, 16833-27-5; tantalum, 7440-25-7; Biocompatible Materials; Membranes, Artificial; Oxides; Tantalum, 7440-25-7References: Goy, J.J., Eeckhout, E., Stauffer, J.C., Vogt, P., Kappenberger, L., Emergency Endoluminal Stenting for Abrupt Vessel Closure following Coronary Angioplasty: A Randomised Comparison of the Wiktor and Palmaz-Schatz Stents (1995) Catheter. Cardiov. Diag., 34 (2), pp. 128-132;Hamasaki, N., Nosaka, H., Kimura, T., Nakagawa, Y., Yokoi, H., Nobuyioshi, M., Initial Experience with the Cordis Stent: Analysis of Serial Angiographic Follow-up (1995) Catheter. Cardiov. Diag., 42 (2), pp. 166-172; 
Hamm, C.W., Beythien, C., Sievert, H., Langer, A., Multicenter Evaluation of the Strecker Tantalum Stent for Acute Coronary Occlusion after Angioplasty (1995) Amer. Heart J., 129 (3), pp. 423-429; 
Strecker, E.-P.K., Boos, I.B.L., G{\"o}ttman, D.V.S., Haase, W., Popliteal Artery Stenting Using Flexible Tantalum Stents (2001) Cardiovasc. Intervent. Radiol., 24 (3), pp. 168-175; 
Rieger, J., Hautmann, H., Linsenmaier, U., Weber, C., Treitl, M., Huber, R.M., Pfeifer, K.-J., Treatment of Benign and Malignant Tracheobronchial Obstruction with Metal Wire Stents: Experience with a Balloon-expandable and a Self Expandable Stent Type (2004) Cardiovasc. Intervent. Radiol., 27 (4), pp. 339-343; 
Bertrand, O.F., Sipehia, R., Mongrain, R., Rodes, J., Tardif, J.C., Bilodeau, L., Cote, G., Bourassa, M.G., Biocompatibility Aspect of New Stent Technology (1998) J. Am. Coll. Cardiol., 32 (3), pp. 562-571; 
Shih, C.-C., Lin, S.-J., Chung, K.-H., Chen, Y.-L., Su, Y.-Y., Increased Corrosion Resistance of Stent Materials by Converting Current Surface Film of Polycrystalline Oxide into Amorphous Oxide (2000) J. Biomed. Mater. Res., 52 (2), pp. 323-332; 
Thierry, B., Tabrizian, M., Trepanier, C., Savadogo, O., Yahia, L.H., Effect of Surface Treatment and Sterilization Processes on the Corrosion Behavior of NiTi Shape Memory Alloy (2000) J. Biomed. Mater. Res., 51 (4), pp. 685-693; 
Macionczyc, F., Gerold, B., Thull, R., Repassivating Tantalum/Tantalum Oxide Surface Modification on Stainless Steel (2001) Surface and Coatings Technology, 142-144, pp. 1085-1087; 
Starovesky, D., Gotman, I., Corrosion of Titanium Nitride Coated Ni-Ti Shape Memory Surgical Implant (2001) Biomaterials, 22 (13), pp. 1853-1859; 
Chen, J.Y., Leng, Y.X., Tian, X.B., Wang, L.P., Huang, N., Chu, P.K., Yang, P., Antithrombogenic Investigation of Surface Energy and Optical Bandgap and Hemocompatibility Mechanism of Ti(Ta(+5))O 2 (2002) Biomaterials, 23 (12), pp. 2545-2552; 
Tan, L., Crone, W.C., Sridharan, K., Fretting Study of Surface Modified Ni-Ti Shape Memory Alloy (2002) J. Mater. Sci. Mater. Med., 13 (5), pp. 501-508; 
Ciss{\'e}, O., Savadogo, O., Wu, M., Yahia, L.H., Effect of Surface Treatment of NiTi Alloy on its Corrosion Behavior in Hank{\textquoteright}s Solution (2002) J. Biomed. Mater. Res., 61 (3), pp. 339-345; 
Schabalovskaya, S., Rondelli, G., Anderegg, J., Simpson, B., Budko, S., Effect of Chemical Etching and Aging in Boiling Water on the Corrosion Resistance of Nitinol Wires with Black Oxide Resulting from Manufacturing Process (2003) J. Biomed. Mater. Res., 66 B (1), pp. 331-340; 
Shih, C.-C., Shih, C.-M., Su, Y.-Y., Lin, S.-J., Impact on the Thrombogenicity of Surface Oxide Properties of 316L Stainless Steel for Biomedical Applications (2003) J. Biomed. Mater. Res., 67 A, pp. 1320-1328; 
Cheng, Y., Cai, W., Li, H.T., Zheng, Y.F., Zhao, L.C., Surface Characteristics and Corrosion Resistance Properties of TiNi Shape Memory Alloy Coated with Tantalum (2004) Surface and Coatings Technology, 186 (3), pp. 346-352; 
Silva, R.A., Marques, M.A., Silveira, A., Walls, M., Rondot, B., Guidoin, R., (1998) Proceedings of the 14th European Conference on Biomaterials, pp. 37-37. , (eds) In: Dutch Society for Biomaterials, September, The Hague; 
Pippen, C.M.J.M., Plenk Jr., H., Ebel, M.F., Svagera, R., Verenisch, J., Characterization of Microblasted and Reactive Ion Etched Surfaces on the Commercially Pure Metals Niobium, Tantalum and Titanium (1997) J. Mater. Sci.: Mater. in Med., 8 (12), pp. 781-784; 
Park, J.B., (1994) Biomaterials Science and Engineering, pp. 217-217. , (ed.) Plenum Press, New York; 
Jonhson, P.F., Bernstein, J.J., Hunter, G., Dawson, W.W., Hench, L.L., An in vitro and in vivo Analysis of Anodised Tantalum Capacitive Electrodes: Corrosion Response, Physiology, and Histology (1997) J. Biomed. Mater. Res., 11 (5), pp. 637-656; 
Black, J., Biological Performance of Tantalum (1994) Clin. Mater., 16 (3), pp. 167-173; 
Stackpool, G.J., Kay, A.B., Morton, P., Harvey, E.J., Tanzer, M., Bone, J.D., (1995) Proceedings of Combined Orthopaedic Research Societies Meeting, Orthopaedic, pp. 45-45. , (eds) In: Research Society Rider Dickerson Inc., Chicago; 
Silva, R.A., Walls, M., Rondot, B., Da Cunha Belo, M., Guidoin, R., Electrochemical and Microstructural Studies of Tantalum and its Oxide Films for Biomedical Applications in Endovascular Surgery (2002) J. Mater. Sci.: Mater. Medicine, 13 (5), pp. 495-500; 
Lohrengel, M.M., Formation of Ionic Space Charge Layers in Oxide Films on Valve Metals (1994) Electrochim. Acta, 39 (8-9), pp. 1265-1271; 
Macagno, V.A., Schultze, J.W., The Growth and Properties of Thin Oxide Layers on Tantalum Electrodes (1984) J. Electroanal. Chem., 180 (1), pp. 157-170; 
Schultze, J.W., Macagno, V.A., Electron Transfer Reaction on Passive Tantalum Electrodes (1986) Electrochim. Acta, 31 (1), pp. 355-363; 
Kerrec, O., Devilliers, D., Groult, H., Chemla, M., Dielectric Properties of Anodic Oxide Films on Tantalum (1995) Electrochim. Acta, 40 (6), pp. 719-724; 
Lewerenz, H.J., Anodic Oxide Films on Silicon (1992) Electrochim. Acta, 37 (10), pp. 847-851; 
Young, L., (1961) Anodic Oxide Films, , Academic Press, London; 
Seah, M.P., Holbourn, M.W., Ortega, C., Davies, J.A., An Intercomparison of Tantalum Pentoxide Reference Studies (1988) Nuclear Instruments and Methods in Physics Research, B30 (2), pp. 128-139
},
	month = {2006///},
	pages = {93 - 103},
	abstract = {Self-expandable nitinol (nickel-titanium) alloys and 316L stainless steel are the most commonly used materials in the production of coronary stents. However, tantalum (Ta) has already been used to make stents for endovascular surgery and may constitute an alternative to other materials because of its better electrochemical performance, namely its higher corrosion resistance, as well as its radio-opacity. The characterization of wet polished, chemically polished, wet polished anodized, and chemically polished anodized Ta electrodes has been performed in a 0.15 M NaCl solution (simulated body fluid) using U                        corr=f(t) measurements, anodic polarizations, capacity measurements, anodic oxidations, and atomic force microscopy (AFM) imaging. Anodic polarization curves have shown that the abnormal current density peak with a maximum value around 1.65 V (critical applied potential, U
                        c) disappeared for the anodized electrodes indicating a probable relationship between the surface states and the film growth. These results are confirmed by capacity measurements. The behavior of wet polished and chemically polished electrodes during anodic oxidations seemingly indicated that for these particular treatments the film growth is different. The AFM images and roughness measurements have shown that chemical polishing produced smoother electrodes, a fact probably related to the differences in film growth. {\textcopyright} 2006 Sage Publications.
},
	keywords = {AFM, Anodic films, Anodic oxide film growth, Anodic polarization, article, artificial membrane, Atomic force microscopy, Biocompatible Materials, biomaterial, Biomedical applications, Biomedical engineering, Chemical polishing, chemistry, Coronary stents, Corrosion resistance, Crystallization, Current density, electric conductivity, Electrochemical properties, electrochemistry, electrode, Electrodes, materials testing, Membranes, Artificial, methodology, oxidation reduction reaction, Oxidation-Reduction, oxide, Oxides, Polarization, Surface Properties, surface property, Surface treatment, Tantalum, Tantalum electrodes},
	isbn = {08853282 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33746412438\&partnerID=40\&md5=e418196142aae805bc91d17a99fcc517},
	author = {Silva, R.A. and Silva, I.P. and Rondot, B.}
}
@article {2420,
	title = {The influence of functional groups of self-assembled monolayers on fibrous capsule formation and cell recruitment},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {76},
	year = {2006},
	note = {Cited By (since 1996): 13Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.30602Language of Original Document: EnglishCorrespondence Address: Barbosa, J.N.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: judite@ibmc.up.ptChemicals/CAS: gold, 7440-57-5; thiol derivative, 13940-21-1; Biocompatible Materials; Gold, 7440-57-5; Sulfhydryl CompoundsReferences: Babensee, J.E., Anderson, J.M., McIntire, L.V., Mikos, A.G., Host response to tissue engineered devices (1998) Adv Drug Deliv Rev, 33, pp. 111-139;Anderson, J.M., In vivo biocompatibility of implantable delivery systems and biomaterials (1994) Eur J Pharm Biopharm, 40, pp. 1-8; 
Anderson, J.M., Biological responses to materials (2001) Annu Rev Mater Res, 31, pp. 81-110; 
Tang, L., Eaton, J.W., Inflammatory responses to biomaterials (1995) Am J Clin Pathol, 103, pp. 466-471; 
Mrksich, M., Whitesides, G.M., Interactions of SAM{\textquoteright}s with proteins (1996) Annu Rev Biophys Biomol Struct, 25, pp. 55-57; 
Ulman, A., Formation and structure of self-assembled monolayers (1996) Chem Rev, 96, pp. 1533-1554; 
Mrkisich, M., Tailored substrates for studies of attached cell culture (1998) Cell Mol Life Sci, 54, pp. 653-662; 
Pan, S., Castner, D.G., Ratner, B.D., Multitechnique surface characterization of derivatization efficiences for hydroxyl-terminated self-assembled monolayers (1998) Langmuir, 14, pp. 3545-3550; 
Sedwick, A.D., Sin, Y.M., Edwards, J.C., Willoughby, D.A., Increased inflammatory reactivity in newly formed lining tissue (1983) J Pathol, 141, pp. 483-495; 
Castro, A.G., Esaguy, N., Macedo, P.M., {\'A}guas, A.P., Silva, M.T., Live but not heat-killed mycobacteria cause rapid chemotaxis of large numbers of eosinophils in vivo and are ingested by the attracted granulocytes (1991) Infect Immun, 59, pp. 3009-3014; 
Isaji, M., Momose, Y., Naito, J., Enhancement of inflammatory reactions in a non-immunological air pouch model in rats (1989) Br J Exp Pathol, 70, pp. 705-716; 
Martins, M.C.L., Naeemi, E., Ratner, B.D., Barbosa, M.A., Albumin adsorption on cibacron blue F3G-A immobilized onto oligo(ethylene glycol)-terminated self-assembled monolayers (2003) J Mater Sci Mater Med, 14, pp. 945-954; 
Lin, J., Chuang, W., Synthesis, surface characterization, and platelet reactivity evaluation for the self-assembled monolayer of alkanethiol with sulfonic acid functionality (2000) J Biomed Mater Res, 51, pp. 413-423; 
Thomsen, P., Gretzer, C., Macrophage interactions with modified materials surfaces (2001) Curr Opin Solid State Mater Sci, 5, pp. 163-176; 
Tegoulia, V.A., Cooper, S.L., Leukocyte adhesion on model surfaces under flow: Effects of surface chemistry, protein adsorption, and shear rate (2000) J Biomed Mater Res, 50, pp. 291-301; 
Tsai, M., Lin, J., Surface characterization and platelet adhesion studies of self-assembled monolayers with phosphonate ester and phosphonic acid functionalities (2001) J Biomed Mater Res, 55, pp. 554-565; 
Ostuni, E., Yan, L., Whitesides, G.M., The interaction of proteins and cells with self-assembled monolayers of alkanethiolates on gold and silver (1999) Colloids Surf B, 15, pp. 3-30; 
Yang, S., Ren, W., Park, Y., Sieving, A., Hsu, S., Nasser, S., Wooley, P.H., Diverse cellular and apoptotic responses to variant shapes of UHMWPE particles in a murine model of inflammation (2002) Biomaterials, 23, pp. 3535-3543; 
Sieving, A., Wu, B., Mayton, L., Nasser, S., Wooley, P.H., Morphological characteristics of total joint arthroplasty-derived ultrahigh molecular weight polyethylene (UHMWPE) wear debris that provoke inflammation in a murine model of inflammation (2003) J Biomed Mater Res A, 64, pp. 457-464; 
Barbosa, J.N., Barbosa, M.A., {\'A}guas, A.P., Inflammatory responses and cell adhesion to self-assembled monolayers of alkanethiolates on gold (2004) Biomaterials, 25, pp. 2557-2563; 
Anderson, D.C., Springer, T.A., Leukocyte adhesion deficiency: An inherited defect in the Mac-1, LFA-1 and p150, 95 glycoproteins (1987) Annu Rev Med, 38, pp. 175-184; 
Murphy, R.T., Foley, J.B., Crean, P., Walsh, M.J., Reciprocal activation of leukocyte-endothelial adhesion molecules in acute coronary syndromes (2003) Int J Cardiol, 90, pp. 247-252; 
Tang, L., Wu, Y., Timmons, R.B., Fibrinogen adsorption and host tissue responses to plasma functionalized surfaces (1998) J Biomed Mater Res, 42, pp. 156-163; 
Barbosa, J.N., Madureira, P., Barbosa, M.A., {\'A}guas, A.P., The attraction of Mac-1 + phagocytes during acute inflammation by methyl-coated self-assembled monolayers (2005) Biomaterials, 26, pp. 3021-3027
},
	month = {2006///},
	pages = {737 - 743},
	abstract = {The contribution of the surface chemistry of an implant to the thickness of the fibrous capsule formed after implantation was herein investigated. For that, self-assembled monolayers (SAMs) of alkanethiols on gold with different terminal functional groups (COOH, OH, and CH                        3) were used. These surfaces were implanted in subcutaneous air pouches of BALB/c mice and the ensuing fibrous capsules were evaluated and compared with the initial inflammatory response caused by the implant. The thickness of the fibrous capsules that are under organization around the implant was measured 1 week after implantation by histology. Inflammatory exudates were collected from the air pouches 24 h after the implantation of SAMs and were analyzed by flow cytometry. A significant increase in the thickness of fibrous capsules was seen around implanted CH
                        3-terminated SAMs, and also in gold surfaces, in comparison with the air pouch wall of sham-operated mice and of COOH- and OH-covered SAMs. The CH
                        3-coated implants also recruited higher numbers of inflammatory cells; this enhancement involved a significant number of Mac-1
                        + cells. Our data indicate that implant surfaces coated with CH
                        3 induce thick fibrous capsules and this may be the result of the stronger inflammatory effect of CH
                        3 in comparison with COOH or OH chemical groups. {\textcopyright} 2005 Wiley Periodicals, Inc.
},
	keywords = {animal, animal cell, animal experiment, animal model, animal tissue, Animals, article, Bagg albino mouse, Biocompatible Materials, biomaterial, chemistry, controlled study, flow cytometry, functional group, gold, histology, implantation, Inflammation, male, Mice, Mice, Inbred BALB C, Monolayers, mouse, nonhuman, Self assembly, Self-assembled monolayers, Sulfhydryl Compounds, Surface chemistry, thiol derivative, tissue reaction, Tissue responses},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33745564665\&partnerID=40\&md5=6cbc5f976e86764eea35724a4af706f9},
	author = {Barbosa, J.N. and Madureira, P. and Barbosa, M.A. and {\'A}guas, A.P.}
}
@article {2407,
	title = {Silicon addition to hydroxyapatite increases nanoscale electrostatic, van der Waals, and adhesive interactions},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {78},
	year = {2006},
	note = {Cited By (since 1996): 15Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.30737PubMed ID: 16646067Language of Original Document: EnglishCorrespondence Address: Ortiz, C.; Department of Materials Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States; email: coritz@mit.eduChemicals/CAS: hydroxyapatite, 1306-06-5, 51198-94-8; silicon, 7440-21-3; thiol derivative, 13940-21-1; Biocompatible Materials; Durapatite, 1306-06-5; Silicon, 7440-21-3References: Carlisle, E.M., Silicon: A possible factor in bone calcification (1970) Science, 167, pp. 279-280;Gibson, I.R., Best, S.M., Bonfield, W., Chemical characterization of silicon-substituted hydroxyapatite (1999) J Biomed Mater Res, 44, pp. 422-428; 
Gibson, I.R., Best, S.M., Bonfield, W., Effect of silicon substitution on the sintering and microstructure of hydroxyapatite (2002) J Am Ceram Soc, 85, pp. 2771-2777; 
Legeros, R.Z., LeGeros, J.P., Dense hydroxyapatite (1993) An Introduction to Bioceramics, pp. 139-180. , Hench LL, Wilson J, editors. Singapore: World Scientific; 
Van Raemdonck, W., Ducheyne, P., De Meester, P., Calcium phosphate ceramics (1984) Metal and Ceramic Biomaterials, pp. 143-166. , Ducheyne P, Hastings G, editors. Boca Raton, FL: CRC Press; 
Botelho, C.M., Lopes, M.A., Gibson, I.R., Best, S.M., Santos, J.D., Structural analysis of Si-substituted hydroxyapatite: Zeta potential and X-ray photoelectron spectroscopy (2002) J Mater Sci: Mater Med, 13, pp. 1123-1127; 
Gibson, I.R., Huang, J., Best, S.M., Bonfield, W., Enhanced in vitro cell activity and surface apatite layer formation on novel silicon-substituted hydroxyapatites (1999) Proceedings of the International Symposium on Ceramics in Medicine, Bioceramics 12, pp. 191-194. , Ohgushi H, Hastings GW, Yoshikawa T, editors. River Edge, NJ: World Scientific Publishing; 
Balas, F., Perez-Pariente, J., Vallet-Regi, M., In vitro bioactivity of silicon-substituted hydroxyapatites (2003) J Biomed Mater Res A, 66, pp. 364-375; 
Botelho, C.M., Brooks, R.A., Best, S.M., Lopes, M.A., Santos, J.D., Rushton, N., Bonfield, W., Biological and physical-chemical characterization of phase pure HA and Si-substituted hydroxyapatite by different microscopy techniques (2004) Proceedings of the International Symposium on Ceramics in Medicine, Bioceramics 16, pp. 845-848. , Barbosa MA, Monteiro FJ, Correia R, Leon B, editors. Uetikon-Zuerich, Switzerland: Trans Tech; 
Patel, N., Best, S.M., Bonfield, W., Gibson, I.R., Hing, K.A., Damien, E., Revel, P.A., A comparative study on the in vivo behavior of hydroxyapatite and silicon substituted hydroxyapatite granules (2002) J Mater Sci: Mater Med, 13, pp. 1199-1206; 
Porter, A.E., Patel, N., Skepper, J.N., Best, S.M., Bonfield, W., Effect of sintered silicate-substituted hydroxyapatite on remodeling process at the bone-implant interface (2004) Biomaterials, 25, pp. 3303-3314; 
Vandiver, J., Dean, D., Patel, N., Bonfield, W., Ortiz, C., Nanoscale variation in surface charge of synthetic hydroxyapatite detected by chemically and spatially specific high resolution force spectroscopy (2005) Biomaterials, 26, pp. 271-283; 
Ducheyne, P., Qiu, Q., Bioactive ceramics: The effect of surface reactivity on bone formation and bone cell function (1999) Biomaterials, 20, pp. 2287-2303; 
Dubok, V.A., Bioceramics - Yesterday, today, tomorrow (2000) Powder Metall Met Ceram, 39, pp. 381-394; 
Bruijn, J.D., Klein, C.P.A.T., De Groot, K., Van Blitterswijk, V.C.A., The ultrastructure of the bone-hydroxyapatite interface in vitro (1992) J Biomed Mater Res, 26, pp. 1365-1382; 
Neo, M., Nakamura, T., Ohtsuki, C., Kokubo, T., Yamamuro, T., Apatite formation on three kinds of bioactive material at an early stage in vivo: A comparative study by transmission electron microscopy (1993) J Biomed Mater Res, 27, pp. 999-1006; 
Kokubo, T., Kushitani, H., Sakka, S., Kitsugi, T., Yamamuro, T., Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W (1990) J Biomed Mater Res, 24, pp. 721-734; 
Himeno, T., Kim, H.-M., Kaneko, H., Kawashita, M., Kokubo, T., Nakamura, T., Surface structural changes of sintered hydroxyapatite in terms of surface charge (2003) Proceedings of the International Symposium on Ceramics in Medicine, Bioceramics 15, pp. 457-460. , Besim Ben-Nissan DS, William Walsh, editors. Uetikon-Zuerich, Switzerland: Trans Tech; 
Sato, K., Kumagai, Y., Tanaka, J., Apatite formation on organic monolayers in simulated body environment (2000) J Biomed Mater Res, 50, pp. 16-20; 
Tanahashi, M., Matsuda, T., Surface functional group dependence on apatite formation on self-assembled monolayers in a simulated body fluid (1997) J Biomed Mater Res, 34, pp. 305-315; 
Yamashita, K., Oikawa, N., Umegaki, T., Acceleration and deceleration of bone-like crystal growth on ceramic hydroxyapatite by electric poling (1996) Chem Mater, 8, pp. 2697-2700; 
Hwang, K.S., Song, J.E., Jo, J.W., Yang, H.S., Park, Y.J., Ong, J.L., Rants, H.R., Effect of poling conditions on growth of calcium phosphate crystal in ferroelectric BaTiO 3 ceramics (2002) J Mater Sci: Mater Med, 13, pp. 133-138; 
Ohgaki, M., Kizuki, T., Katsura, M., Yamashita, K., Manipulation of selective cell adhesion and growth by surface charges of electrically polarized hydroxyapatite (2001) J Biomed Mater Res, 57, pp. 366-373; 
Kobayashi, T., Nakamura, S., Yamashita, K., Enhanced osteobonding by negative surface charges of electrically polarized hydroxyapatite (2001) J Biomed Mater Res, 57, pp. 477-484; 
Parsegian, V.A., Gingell, D., On the electrostatic interaction across a salt solution between two bodies bearing unequal charges (1972) Biophys J, 12, pp. 1192-1204; 
Butt, H.J., Measuring electrostatic, van der Waals, and hydration forces in electrolyte-solutions with an atomic force microscope (1991) Biophys J, 60, pp. 1438-1444; 
Brown, P.W., Martin, R.I., An analysis of hydroxyapatite surface layer formation (1999) J Phys Chem B, 103, pp. 1671-1675; 
Noy, A., Vezenov, D.V., Lieber, C.M., Chemical force microscopy (1997) Annu Rev Mater Sci, 27, pp. 381-421; 
Israelachvili, J., (1991) Intermolecular and Surface Forces, , Amsterdam: Academic Press; 
Verwey, E.J.W., Overbeek, J.T.G., Nes, K.V., (1948) Theory of the Stability of Lyophobic Colloids, , New York: Elsevier; 
Akao, M., Aoki, H., Kato, K., Mechanical properties of sintered hydroxyapatite for prosthetic applications (1981) J Mater Sci, 16, pp. 809-812; 
Seog, J., Dean, D., Plass, A.H.K., Wong-Palms, S., Grodzinsky, A.J., Ortiz, C., Direct measurement of glycosaminoglycan intermolecular interactions via high-resolution force spectroscopy (2002) Macromolecules, 35, pp. 5601-5615; 
Ashby, P.D., Chen, L., Lieber, C.M., Probing intermolecular forces and potentials with magnetic feedback chemical force microscopy (2000) J Am Chem Soc, 122, pp. 9467-9472; 
Ederth, T., Computation of Lifshitz-van de Waals forces between alkylthiol monolayers on gold films (2001) Langmuir, 17, pp. 3329-3340; 
Forsythe, G.E., Wasow, W.R., (1960) Finite Difference Methods for Partial Differential Equations, , New York: Wiley; 
(1997) Principles of Colloid and Surface Chemistry, 3rd Ed., , Hiemenz P, Rajagopalan R, editors. New York: Marcel Dekker; 
Eichert, D., Combes, C., Drouet, C., Rey, C., Formation and evolution of hydrated surface layers of apatites (2005) Proceedings of the International Symposium on Ceramics in Medicine, Bioceramics 17, pp. 3-6. , Li P, Zhang K, Colwell C, editors. Uetikon-Zuerich, Switzerland: Trans Tech; 
Rixman, M., Dean, D., Macias, C., Ortiz, C., Nanoscale intermolecular interactions between human serum albumin and alkanethiol self-assembled monolayers (2003) Langmuir, 19, pp. 6202-6218; 
Ackler, H.D., French, R.H., Chiang, Y., Comparisons of Hamaker constants for ceramic systems with intervening vacuum or water: From force laws and physical properties (1996) J Colloid Interface Sci, 179, pp. 460-469; 
Kane, V., Mulvaney, P., Double-layer interactions between self-assembled monolayers of ω-mercaptoundecanoic acid on gold surfaces (1998) Langmuir, 14, pp. 3302-3311; 
Atkins, P., (1997) The Elements of Physical Chemistry, , New York: Freeman; 
Zhu, P.X., Masuda, Y., Koumoto, K., Site-selective adhesion of hydroxyapatite microparticles on charged surfaces in a super-saturated solution (2001) J Colloid Interface Sci, 243, pp. 31-36; 
James, M., Hunter, R.J., O{\textquoteright}Brien, R.W., Effect of particle size distribution and aggregation on electroacoustic measurements of ξ potential (1992) Langmuir, 8, pp. 420-423; 
Toikka, G., Hayes, R.A., Ralston, J., Surface forces between spherical ZnS particles in aqueous electrolyte (1996) Langmuir, 12, pp. 3783-3788; 
Pedersen, H.G., Bergstrom, L., Forces measured between zirconia surfaces in poly(acrylic acid) solutions (1999) J Am Ceram Soc, 82, pp. 1137-1145; 
Cohen, M., Joester, D., Geiger, B., Addadi, L., Spatial and temporal sequence of events in cell adhesion: From molecular recognition to focal adhesion assembly (2004) Chem Bio Chem, 5, pp. 1393-1399; 
Smith, I.O., Baumann, M.J., McCabe, L.R., Electrostatic interactions as a predictor for osteoblast attachment to biomaterials (2004) J Biomed Mater Res A, 70, pp. 436-441; 
(1979) Plasma Proteins, , Blombaek B, Hanson L, editors. New York: Wiley; 
Porter, A.E., Hobbs, L.W., Rosen, V.B., Spector, M., The ultrastructure of the plasma-sprayed hydroxyapatite-bone interface predisposing to bone bonding (2002) Biomaterials, 23, pp. 725-733; 
Porter, A.E., Patel, N., Skepper, J.N., Best, S.M., Bonfield, W., Comparison of in vivo dissolution processes in hydroxyapatite and silicon-substituted hydroxyapatite bioceramics (2003) Biomaterials, 24, pp. 4609-4620
},
	month = {2006///},
	pages = {352 - 363},
	abstract = {The normal intersurface forces between nanosized probe tips functionalized with COO},
	keywords = {Adhesives, article, Atomic force microscopy, Biocompatible Materials, biomaterial, Biomaterials, Bone, cell adhesion, chemical binding, chemical interaction, derjaguin landau verwey overbeek approximation, Durapatite, electrochemical analysis, Electrostatic, Electrostatics, high resolution force spectroscopy, hydroxyapatite, materials testing, mathematical analysis, Microscopy, Electron, Scanning, Monolayers, Nanomechanics, Nanostructures, Particle Size, Poisson distribution, Polycrystalline materials, Silicon, Silicon-substituted hydroxyapatite, Spectrophotometry, Spectroscopy, Spectroscopy, Fourier Transform Infrared, Stress, Mechanical, Surface charge, surface property, thiol derivative, Van der Waals, Van der Waals forces},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33745965657\&partnerID=40\&md5=df44bade2fb0a3af786b193c7f42d71a},
	author = {Vandiver, J. and Dean, D. and Patel, N. and Botelho, C. and Best, S. and Santos, J.D. and Lopes, M.A. and Bonfield, W. and Ortiz, C.}
}
@article {2453,
	title = {Bone substitutes: General concepts and "state of the art"},
	journal = {Substitutos {\'o}sseos: Conceitos gerais e estado actualArquivos de MedicinaArq. Med.},
	volume = {19},
	year = {2005},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: ARQMELanguage of Original Document: PortugueseCorrespondence Address: Gutierres, M.; Servi{\c c}o de Ortopedia, Hospital de S{\~a}o Jo{\~a}o, Alameda Prof. Hern{\^a}ni Monteiro, 4200-319 Porto, Portugal; email: m.gutierres@sapo.ptChemicals/CAS: calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; calcium sulfate, 13397-24-5, 23296-15-3, 7778-18-9; fibroblast growth factor, 62031-54-3; transforming growth factor, 76057-06-2References: Laurencin, C.T., Chapter I- Bone graft and bonegraft substitutes a brief history (2004) Bone Graft Substitute - ASTM International, , Laurencin CT, editors;Ratner, B.D., A history of Biomaterials (2004) Biomaterials Science. 2 nd Edition, pp. 10-19. , Ratner, edits. Elsevier Inc; 
Williams, Confer{\^e}nciade consensos sobre defini{\c c}{\~o}es em Biomateriais (1991) Chester; 
Mainard, (2001) Les Substituts Osseox Em 2001: Monographie Edit{\'e}e Par GESTO, , (Association pour L{\textquoteright}etude des Greffes et substitutes Tissulaires en Orthop{\'e}die) sous la direction de Dr. Mainard, Edition Romillat, Paris; 
Banwart, J.C., Iliac crest bone graft harvest donor site morbidity (1995) Spine, 20, pp. 1055-1060; 
Tomford, W.W., Bone allografts: Past, Present and future (2000) Cell Tissue Bank, 1, pp. 105-109; 
Vaccaro, A.R., The role of the osteoconductive scaffold in synthetic bone graft (2002) Orthopedics, 25 (SUPPL. 5), pp. S71-S78; 
Khan, S.N., Clinical applications of Bone Graft substitutes (2000) Orthop Clin North Am, 31, pp. 389-398; 
Nishida, K., Gilbertson, L.G., Ch, E., Kang, J.D., Potential applications of gene therapy to the treatment of spinal disorders (2000) Spine, 25, pp. 1308-1314; 
Proubasta, J., Mur, J.G., Planell, J.A., Biocompatibilidad, materiales implantables, tipos de implante (1997) Fundamentos de Biomec{\^a}nica Y Biomateriales, pp. 271-350. , Ediciones Ergon. Madrid; 
Davies, J.E., Histodynamics of endosseous wound healing (2000) Bone Engineering, pp. 1-11. , Davies JE, editors. Canada: EM2; 
Anderson, Biological responses to materials (2001) Annu Rev Mater Res, 31, pp. 81-110; 
Bauer, T.W., Muschler, G.F., Bone Graft Materials (2000) Clin Orthop, 37, pp. 10-27; 
Fleming Jr., J.E., Cornell, C.N., Muschler, G.F., Bone cells and matrices in Orthopedic Tissue Engineering (2000) Orthop Clin North Am, 31, pp. 357-374; 
Vaccaro, A.R., Chiba, K., Heller, J.G., Bone grafting alternatives in spinal surgery (2002) Spine J, 2, pp. 206-215; 
Boden, S.D., Biology of lumbar spine fusion and use of bone graft substitutes: Present, future and next generation (2000) Tissue Eng, 6, pp. 383-399; 
Burkus, J.K., Sandhu, H.S., Gornet, M.F., Longley, M.C., Use of rh BMP-2 in combination with structural control allografts; Clinical and radiographic outcomes in anterior lumbar spinal surgery (2005) J Bone Joint Surg, 87, pp. 1205-1212; 
Keller, E.E., Triplett, W.W., Iliac bone graft: Review of 160 consecutive cases (1987) J Oral Maxillofac Surg, 45, pp. 11-14; 
Younger, E., Chapmann, M., Morbidity at bone graft-donor sites (1989) J Orthop Trauma, 3, pp. 192-195; 
Montgomery, D.M., Aronson, D.D., Lee, C.L., Lamont, R.L., Posterior spinal fusion: Allograft versus autografts bone (1990) J Spine Disord, 3, pp. 370-375; 
Mc Carthy, R.E., Peek, R.D., Morrisy, R.T., Hough Jr., A.J., Allograft bone in spinal fusion for paralytic scoliosis (1986) J Bone Joint Surg Am, 68, pp. 70-75; 
Lane, J.M., Safdar, N.K., Bone Grafts of the 20th Century: Multiple purposes, materials and goals (2000) Orthopedics Today; 
Connolly, J., Guse, R., Lippiello, L., Development of an osteogenic bone marrow preparation (1989) J Bone Joint Surg Am, 71, pp. 684-691; 
Pettier, L.F., The use of plaster of Paris to fill defects in bone (1961) Clin Orthop, 21, pp. 1-31; 
Hogset, O., Bredberg, G., Plaster of Paris and hair cell morphology. A scanning electron microscopic study of an alternative implant materials for ear surgery (1988) Acta Otolaryngol, 106, pp. 331-338; 
Peltier, L.F., Jones, R.H., Treatment of unicameral bone cysts by curetage and packing with plaster of Paris pellets (2004) Clin Orthop, 422, pp. 145-147; 
Hench, L.L., Best, S., Ceramic, glasses and glass ceramics (2004) Biomaterial Sciences. 2 nd Edition, pp. 155-170. , Ratner BD, editors; 
Hench, L.L., Wilson, J., Surface active biomaterials (1984) Science, 226, pp. 630-636; 
Boyan, B.D., Nasatzky, E., Keller, T.A., Schartz, Z., Substitutes del injerto osseo (1998) Curr Opin Orthop, 3, pp. 17-23; 
Clark, A.E., Hench, L.L., Paschall, H.A., The influence of surface chemistry on implant interface histology: A theorical basis for implant materials selection (1976) J Biomed Mater Res, 110, pp. 161-171; 
Hench, L.L., Wilson, J., Bioactive glasses: Present and future (1998) Bioceramics, 11, pp. 31-36; 
Gatti, A.M., Zaffe, D., Bioactive glasses and chemical bond (1992) Biomaterials, pp. 97-106; 
Hamadouche, M., Sedel, L., Ceramics in Orthopaedics (2000) J Bone Joint Surg Br, 82, pp. 1095-1099; 
Oonishi, H., Hench, L.L., Wilson, I., Quantitative comparison of bone growth behavior in ceramics of Bioglass{\textregistered}, A-W glass ceramics and HA (2000) J Biomed Mater Res, 51, pp. 37-46; 
Oonishi, H., Kushitani, S., Yasukawa, E., Particulate bioglass compared with hydroxyapatite as a bone graft substitute (1997) Clin Orthop Rel Research, 334, pp. 316-325; 
Kokubo, T., Ito, S., Huang, Z., Ca-P - Rich Layer formed on high strength bioactive glass ceramics A-W (1990) J Biomed Mater Res, 24, pp. 331-343; 
Vogel, W., Holland, H., Gummel, J., Development of machineable bioactive glass-ceramics for medical uses (1986) Journal of Non-Crystaline Solids, 80, pp. 34-51; 
Holland, W., Vogel, W., Mortier, W.J., Duvingneaud, P.H., Naessens, G., Plumat, E., A new type of crystal in machineable glass-ceramics (1983) Glass Technology, 24, pp. 318-322; 
Burnie, J., (1988) Controlled Release Glass (C. R. G.) - A New Biomaterial, , PhD Thesis; 
Driessens, F.C.M., Ramselaar, M.M.A., Chemical reactions of calcium phosphate implants after implantation in vivo (1992) J Mater Sci, 3, pp. 413-417; 
Betz, R.R., Limitation of autograft and allograft new synthesis solutions (2002) Orthopedics, (SUPPL. 5), pp. S561-S570; 
Cornell, C.N., Osteoconductive materials and their role as substitutes for autogenous bone grafts (1999) Orthop Clin North Am, 30, pp. 599-608; 
Lascart, T., Favard, L., Burdin, P., Traore, O., Utilisation du phosphate tricalcique dans les osteotomies tibiales de valgisation par addition interne (1998) Ann Orthopediques de L{\textquoteright} Ouest, 30, pp. 137-141; 
Meynet, J., Osteotomie tibiale de valgisation par ouverture interne: Place des substituts osseux (1998) Ann Orthopediques de L{\textquoteright}Ouest, 30, pp. 171-173; 
Gaasbeek, R., Toonen, H., Heerwaarden, R., Buma, P., Mechanism of bone incorporation of β-TCP bone substitute in open wedge tibial osteotomy in patients (2005) Biomat Erials, 26 (33), pp. 6713-6719; 
Yamamoto, T., Onga, T., Marui, T., Mizuno, K., Use of Hydroxyapatite to fill cavities after excision of begin bone tumors: Clinical results (2000) J Bone Joint Surg, 82 B, pp. 1117-1120; 
Koshino, T., Murase, T., Saito, T., Medial opening-wedge high tibial osteotomy with use of porous hydroxyapatite to treat medial compartment osteoarthritis of the knee (2003) J Bone Joint Surg Am, 85, pp. 78-85; 
Regner, L., Carlsson, L., Karrholm, J., Herbert, P., Ceramic coating improves tibial component fixation in total knee arthroplasty (1998) J Arthroplasty, 13, pp. 882-889; 
Tadic, D., Epple, M., A thorough physicochemical characterisation of 14 calcium phosphate-based bone substitution materials in comparison to natural bone (2004) Biomaterials, 25, pp. 987-994; 
Santos, J.D., Hastings, G.W., Knowles, J.C., Sintered hydroxyapatite compositions and method for the preparation thereof, European Patent WO 0068164. 1999Lopes, M.A., (1999) Glass Reinforced Hydroxyapatite Composites: Structure, Physico Chemical Characterization and Biological Performance, , PhD Thesis. FEUP; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inove, H., Osteocompatibility and in vivo evaluation of glass reinforced hydroxyapatite composite (1999) Bioceramics, 12, pp. 421-424; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Glass reinforced hydroxyapatite composites: Secondary phase proportions and densification effects on bioactive bending strength (1999) J Biomed Mater Res, 48, pp. 734-740; 
Santos, J.D., Vasconcelos, M., Reis, R.L., Afonso, A., Monteiro, F.J., Hastings, G.H., Glass reinforced hydroxyapatite composites: Physical properties and preliminary histological studies in rabbits (1994) Bioceramics, 7, pp. 243-248; 
Afonso, A., Santos, J.D., Vasconcelos, M., Branco, R., Cavalheiro, J., Granules of osteoapatite and glass-reinforced hydroxyapatite implanted in rabbit tibia (1996) J Mat Scien: Materials in Medicine, 7, pp. 507-510; 
Costa, M.A., Gutierres, M., Almeida, L., Lopes, M.A., Santos, J.D., Fernandes, M.H., In vitro mineralization of human bone marrow cells cultured on Bonelike{\textregistered} (2004) Key Engineering Materials, 254-256, pp. 821-824; 
Ferraz, M.P., Fernandes, M.H., Cabral, T., Santos, J.D., Monteiro, F.J., In vitro growth and differentiation of osteoblast-like human bone marrow cells on glass reinforced hydroxyapatite plasma-sprayed coatings (1999) J Mat Scien: Materials in Medicine, 10, pp. 567-576; 
Ferraz, M.P., Knowles, J.C., Olsen, I., Monteiro, F.J., Santos, J.D., Flow cytometry analysis of the effects of pre-immersion on the biocompatibility of glass-reinforced hydroxyapatite plasma-sprayed coatings (2000) Biomaterials, 21, pp. 813-820; 
Gutierres, M., Hussain, N.S., Afonso, A., Biological behaviour of bonelike{\textregistered} graft implanted in the tibia of humans (2005) Key Engineering Materials, 284-286, pp. 1041-1044; 
Knnack, D., Goad, M., Rey, C., Tofigni, A., Chakravarthy, P., Lee, D., Resorbable calcium phosphate bone substitute (1998) J Biomed Mater Res, 43, pp. 399-409; 
Kopylou, P., Jonsson, K., Thorngren, K.G., Injectable calcium phosphate in the treatment of distal radius fractures (1996) J Hand Surg Br, 21, pp. 768-771; 
Schreiber, R.E., Blease, K., Ambrosio, A., Amburn, E., Sosnowski, B., Sampath, T.K., Bone induction by ADBMP-2/collagen implants (2005) J Bone Joint Surg Am, 87, pp. 1059-1068; 
Muschler, G.F., Negami, S., Hyodo, A., Gaisser, D., Gasley, K., Kambic, H., Evaluation of collagen ceramic composite graft materials in a spinal fusion model (1996) Clin Orthop, 328, pp. 250-260; 
Chapman, M.W., Bucholz, R., Cornell, C., Treatment of acute fractures with a collagen calcium phosphate graft material. A Randomized clinical trial (1997) J Bone Joint Surg Am, 79, pp. 495-502; 
Hollinger, J.O., Brekke, J., Gruskin, E., Lee, D.L., Role of bone substitutes (1996) Clin Orthop, 324, pp. 55-65; 
Kelly, E.B., New frontiers in bone grafting (2000) Orthop Techn Review, 2, pp. 1-5; 
Mathes, D.W., Randolph, M.A., Lee, W.P., Strategies for tolerance induction to composite tissue allografts (2000) Microsurgery, 20, pp. 448-452; 
Boyce, T., Edwards, J., Scarborough, N., Allograft bone: The influence of processing on safety and performance (1999) Orthop Clin North Am, 30, pp. 571-581; 
Peterson, B., Whang, P.G., Iglesias, R., Wang, J.C., Lieberman, J.R., Osteoinductivity of commercially available demineralized bone matrix (2004) J Bone Joint Surg Am, 86, pp. 2243-2250; 
Morone, M.A., Boden, S.D., Experimental posterolateral lumbar spinal fusion with a demineralized bone matrix cell (1998) Spine, 23, pp. 159-167; 
Elves, M.W., Salama, R., A study of the development of cytotoxic antibodies produced in recipients of xenografts of iliac bone (1974) J Bone Joint Surg Br, 56, pp. 331-339; 
Urist, M.R., Bone: Formation by autoinduction (1965) Science, 150, pp. 893-899; 
Yuan, H., Zou, P., Yang, Z., Zhang, X., De Bruijn, J.D., Groot, K.D., Bone morphogenic protein and ceramic-induced osteogenesis (1998) J Mat Scien: Materials in Medicine, 9, pp. 717-721; 
Reddi, A.H., Marshall, R., Urist: A renaissance scientist and orthopedic surgeon (2003) J Bone Joint Surgery Am, 85, pp. 3-7; 
Khan, S.N., Bostrom, M.P., Lane, J., Bone growth factors (2000) Orthop Clin North Am, 31, pp. 375-387; 
Reddi, A.H., Bone morphogenetic proteins: From basic science to clinical applications (2001) J Bone Joint Surg Am, 83 A (SUPPL. 1 AND PART 1), pp. S1-S6; 
Sugi, Y., Yamamura, H., Okagawa, H., Markwald, R.R., Bone morphogenetic protein-2 can mediate myocardial regulation atrioventricular cushion mesenchymal cell formation in mice (2004) Dev Biol, 269, pp. 505-518; 
Asahina, I., Sampath, T.K., Nishimura, I., Hauschka, P.V., Human osteogenic protein-1 induces both chondroblastic and osteoblastic differentiation of osteoprogenitor cells derived from newborn rat calvaria (1993) J Cell Biol, 123, pp. 921-933; 
Friedlander, G.E., Perry, C.R., Cole, J.D., Osteotomic protein-1 (BMP-7) in the treatment of tibial nonunions (2001) J Bone Joint Surg Am, 83 A (SUPPL. 1 AND PART 2), pp. S151-S158; 
Boden, S.D., Zdeblick, T.A., Sandhu, H.S., Heim, S.E., The use of Rh BMP-2 in interbody fusion cases. Definitive evidance of osteoinduction in humans: A preliminary report (2000) Spine, 25, pp. 376-386; 
Matsumoto, T., Okazaki, M., Inove, M., Hydroxyapatite particles as a controlled release carrier of protein (2004) Biomaterials, 25, pp. 3807-3812; 
Janssens, K., Dijke, P., Janssens, S., Hul, W.V., Transforming growth factor-beta1 to the bone (2005) Endocr Rev, 26, pp. 743-774; 
Pepper, M.S., Transforming growth factor-beta: Vasculogenesis, angiogensis and vessel wall integrity (1997) Cytokine Growth Factor Rev, 8, pp. 21-43; 
Letterio, J.J., Roberts, A.B., Regulation of immune responses by TGF-beta (1998) Annu Rev Immunol, 16, pp. 137-161; 
O{\textquoteright}Kane, S., Ferguson, M.W., Transforming growth factor beta and wound healing (1997) Int J Biochem Cell Biol, 29, pp. 63-78; 
Aufdemorte, T.B., Fox, W.C., Holt, G.R., McGuff, H.S., Ammann, A.J., Beck, L.S., An intraosseous device for studies of bone-healing. The effect of transforming growth factor beta J Bone Joint Surg Am, 74, pp. 1153-1161; 
Siegel, P.M., Massague, J., Cytostatic and apoptotic actions of TGF-beta in homeostasis and cancer (2003) Nat Rev Cancer, 3, pp. 807-821; 
Derynck, R., Akhurst, R.J., Balmain, A., TGF-beta signaling in tumor suppression and cancer progression (2001) Nat Genet, 29, pp. 117-129; 
Hock, J.M., Canalis, E., Platelet derived growth factor enhances bone cell replication, but not differentiate function of osteoblasts (1994) Endocrinol, 134, pp. 1423-1428; 
Nash, T.J., Howlett, C.R., Martin, C., Steel, J., Johnson, K.A., Hicklin, D.J., Effect of platelet-derived growth factors on tibial osteotomies in rabbits (1994) Bone, 5, pp. 203-208; 
Marx, R.E., Carlson, E.R., Eichstaedt, R.M., Platelet-rich plasma: Growth factor enhancement for bone grafts (1998) Oral Surg, 85, pp. 638-646; 
Trippel, S.B., Courts, R.D., Einhorn, T.A., Mundy, G.R., Rosenfeld, R.G., Instructional course lectures, the American Academy of Orthopaedic Surgeons - Growth factors as therapeutic agents (1996) J Bone Joint Surg Am, 78, pp. 1272-1286; 
Koskinen, E.V., Lindholm, R.V., Nieminen, R.A., Puranen, J.P., Attila, U., Human growth hormone in delayed union and non-union of fractures (1978) Int Orthop, 1, pp. 317-322; 
Northmore-Ball, M.D., Wood, M.R., Meegitt, B.F., A biomechanical study of the effects of growth hormone in experimental fracture healing (1980) J Bone Joint Surg Br, 62, pp. 391-396; 
Ryaby, J.T., Fitzsimmons, R.J., Khin, N.A., Agrowth factor dependent model for magnetic field regeneration of bone formation (1994) Trans Orthop Res Soc, 19, p. 518; 
Jin Gushi, S., Heydmann, A., Kana, S.K., Macey, L.R., Bolander, M.E., Acidic fibroblast growth factor (aFGF) injection stimulates cartilage enlargement and inhibits cartilage gene expression in rat fracture healing (1990) J Orthop Res, 8, pp. 364-371; 
Kawaguchi, H., Kurokawa, T., Hanada, K., Stimulation of fracture repair by recombinant human basic fibroblast growth factor in normal and streptozotocin - Diabetic rats (1994) Endocrinol, 135, pp. 774-781; 
Wang, J.S., Aspenberg, P., Basic fibroblast growth factor and bone induction in rats (1993) Acta Orthop Scandinavia, 64, pp. 551-569; 
Ludwig, S.C., Boden, S.D., Osteoinductive bone graft substitutes for spinal fusion (1999) Orthop Clin North Am, 30, pp. 635-645; 
(1993) An Introduction to Bioceramics, 1. , Hench LL, Wilson J, edits. Advanced series in Ceramics; 
Kingery, W.D., Bowen, H.K., Uhlmann, D.R., (1976) Introduction to Ceramics. 2 nd Ed., , New York: John Wiley \& Sons; 
(1991) Ceramic and Glasses. Engineered Materials Handbook, p. 4. , Schneider SJ, edits. ASM International
},
	month = {2005///},
	pages = {153 - 162},
	abstract = {Bone grafts are nowadays the subject of extensive research worldwide, aimed at overcoming limitations associated with the use of autografts or bone banks to fill bone defects. Additionally, the use of osteogenic substances opens new perspectives in the treatment of fractures, pseudarthroses and vertebral arthrodeses. Due to the large amount of information available in the area of bone grafts, this paper aims not only to revise general concepts but also to summarize what has been published recently in the area of bone substitutes for bone regeneration. {\textcopyright}ArquiMed, 2006.},
	keywords = {Autografts, biomaterial, bone defect, bone demineralization, bone development, Bone graft, Bone growth factors, Bone morphogenetic protein, bone prosthesis, calcium phosphate, calcium sulfate, cortical bone, fibroblast growth factor, fracture, health care facility, health status, human, hydroxyapatite, platelet derived growth factor, pseudarthrosis, publication, review, somatomedin C derivative, spondylarthritis, transforming growth factor},
	isbn = {08713413 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33748855173\&partnerID=40\&md5=494dbb696981df5711e7dda7d1393224},
	author = {Gutierres, M. and Ascens{\~a}o Lopes, M. and Sooraj Hussain, N. and Trigo Cabral, A. and Almeida, L. and Domingos Santos, J.}
}
@article {2455,
	title = {Inflammatory cell recruitment and adhesion to methyl-terminated self-assembled monolayers: Effect of implantation time},
	journal = {Microscopy Research and TechniqueMicrosc. Res. Tech.},
	volume = {66},
	year = {2005},
	note = {Cited By (since 1996): 4Export Date: 21 November 2012Source: ScopusCODEN: MRTEEdoi: 10.1002/jemt.20141PubMed ID: 15816035Language of Original Document: EnglishCorrespondence Address: Barbosa, J.N.; INEB, Inst. de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: judite@ibmc.up.ptChemicals/CAS: gold, 7440-57-5; thiol derivative, 13940-21-1; Coated Materials, Biocompatible; Gold, 7440-57-5References: Anderson, J.M., Biological responses to materials (2001) Annu Rev Mater Res, 31, pp. 81-110;Babensee, J.E., Anderson, J.M., Mclntire, L.V., Mikos, A.G., Host response to tissue engineered devices (1998) Adv Drug Deliv Rev, 33, pp. 111-139; 
Barbosa, J.N., Barbosa, M.A., {\'A}guas, A.P., Inflammatory responses and cell adhesion to self-assembled monolayers of alkanethiolates on gold (2004) Biomaterials, 25, pp. 2557-2563; 
Castro, A.G., Esaguy, N., Macedo, P.M., {\'A}guas, A.P., Silva, M.T., Live but not heat-killed mycobacteria cause rapid chemotaxis of large numbers of eosinophils in vivo and are ingested by the attracted granulocytes (1991) Infect Immun, 59, pp. 3009-3014; 
Gelb, H., Schumacher, H.R., Cuckler, J., Ducheyne, P., Baker, D.G., In vivo inflammatory response to polymethylmethacrylate particulate debris: Effect of size, morphology, and surface area (1994) J Orthop Res, 12, pp. 83-92; 
Hooper, K.A., Nickolas, T.L., Yurkow, E.J., Kohn, J., Laskin, D., Characterization of the inflammatory response to biomaterials using a rodent air pouch model (2000) J Biomed Mater Res, 50, pp. 365-374; 
Hu, W., Eaton, J.W., Tang, L., Molecular basis of biomaterial-mediated foreign body reactions (2001) Blood, 98, pp. 1231-1238; 
Isaji, M., Momose, Y., Naito, J., Enhancement of inflammatory reactions in a non-immunological air pouch model in rats (1989) Br J Exp Pathol, 70, pp. 705-716; 
K{\"a}lltorp, M., Oblogina, S., Jacobsson, S., Karlsson, A., Tengvall, P., Thomsen, P., In vivo cell recruitment, cytokine release and chemiluminescence response at gold, and thiol functionalized surfaces (1999) Biomaterials, 20, pp. 2123-2137; 
Kao, W.J., Evaluation of leukocyte adhesion on polyurethanes: The effects of shear stress and blood proteins (2000) Biomaterials, 21, pp. 2295-2303; 
Kao, W.J., Liu, Y., Gundloori, R., Li, J., Lee, D., Einerson, N., Burmania, J., Stevens, K., Engineering endogenous inflammatory cells as delivery vehicles (2002) J Control Release, 78, pp. 219-233; 
Lin, J., Chuang, W., Synthesis, surface characterization, and platelet reactivity evaluation for the self-assembled monolayer of alkanethiol with sulfonic acid functionality (2000) J Biomed Mater Res, 51, pp. 413-423; 
Lindblad, M., Lestelius, M., Johansson, A., Tengvall, P., Thomsen, P., Cell and soft tissue interactions with methyl- and hydroxyl-terminated alkane thiols on gols surfaces (1997) Biomaterials, 18, pp. 1059-1068; 
Margel, S., Vogler, E.A., Firment, L., Watt, T., Haynie, S., Sogah, D.Y., Peptide, protein, and cellular interactions with self-assembled monolayer model surfaces (1993) J Biomed Mater Res, 27, pp. 1463-1476; 
Martins, M.C.L., Naeemi, E., Ratner, B.D., Barbosa, M.A., Albumin adsorption on cibacron blue F3G-A immobilized onto oligo(ethylene glycol)-terminated self-assembled monolayers (2003) J Mater Sci Mater Med, 14, pp. 945-954; 
Mrksich, M., Using self-assembled monolayers to understand the biomaterials interface (1997) Curr Opin Colloid Int Sci, 2, pp. 83-88; 
Mrkisich, M., Tailored substrates for studies of attached cell culture (1998) Cell Mol Life Sci, 54, pp. 653-662; 
Mrksich, M., Whitesides, G.M., Interactions of SAMs with proteins (1996) Annu Rev Biophys Biomol Struct, 25, pp. 55-57; 
Pan, S., Castner, D.G., Ratner, B.D., Multitechnique surface characterization of derivatization efficiences for hydroxyl-terminated self-assembled monolayers (1998) Langmuir, 14, pp. 3545-3550; 
Prime, K.L., Whitesides, G.M., Self assembled organic monolayers: Model systems for studying the adsorption of proteins at surfaces (1991) Science, 252, pp. 1164-1167; 
Sedwick, A.D., Sin, Y.M., Edwards, J.C., Willoughby, D.A., Increased inflammatory reactivity in newly formed lining tissue (1983) J Pathol, 141, pp. 483-495; 
Tang, L., Eaton, J.W., Mechanisms of acute inflammatory response to biomaterials (1994) Cells and Materials, 4, pp. 429-436; 
Tang, L., Eaton, J.W., Inflammatory responses to biomaterials (1995) Am J Clin Pathol, 103, pp. 466-471; 
Tang, L., Ugarova, T.P., Plow, E.F., Eaton, J.W., Molecular determinants of acute inflammatory responses to biomaterials (1997) J Clin Invest, 97, pp. 1329-1334; 
Tang, L., Jennings, T.A., Eaton, J.W., Mast cells mediate acute inflammatory responses to implanted biomaterials (1998) Proc Natl Acad Sci, 95, pp. 8841-8846; 
Tang, L., Liu, L., Elwing, H.B., Complement activation and inflammation triggered by model biomaterial surfaces (1998) J Biomed Mater Res, 41, pp. 333-340; 
Tegoulia, V.A., Cooper, S.L., Leukocyte adhesion on model surfaces under flow: Effects of surface chemistry, protein adsorption, and shear rate (2000) J Biomed Mater Res, 50, pp. 291-301; 
Tomczok, J., Sliwa-Tomczok, W., Klein, C.L., Van Kooten, T.G., Kirkpatrick, C.J., Biomaterial-induced alterations of human neutrophils under fluid shear stress: Scanning electron microscopical study in vitro (1996) Biomaterials, 17, pp. 1359-1367; 
Ulman, A., Formation and structure of self-assembled monolayers (1996) Chem Rev, 96, pp. 1533-1554; 
Wooley, P.H., Mayton, L., Sud, S., Nasser, S., Synergistic inflammatory responses to metallic and polymeric particles in the murine air pouch model of aseptic loosening (1996) Arthritis Rheum, 39, pp. 175-183; 
Wooley, P.H., Morren, R., Andary, J., Sud, S., Yang, S., Mayton, L., Markel, D., Nasser, S., Inflammatory responses to orthopaedic biomaterials in the murine air pouch (2002) Biomaterials, 23, pp. 517-526; 
Yun, J.K., DeFife, K., Colton, E., Stack, S., Azeez, A., Cahalan, L., Verhoeven, M., Anderson, J.M., Human monocyte/macrophage adhesion and cytokine production on surface-modified poly (tetrafluoroethylene/hexafluoropropylene) polymers with and without protein preadsorption (1995) J Biomed Mater Res, 29, pp. 257-268
},
	month = {2005///},
	pages = {37 - 42},
	abstract = {The contribution of methyl groups in implant-triggered inflammation was investigated in vivo using self-assembled monolayers (SAMs) of alkanethiols on gold. The CH                        3-coated implants were inserted in an air-pouch cavity induced in BALB/c mice. The in situ inflammatory response was monitored 24, 48, and 72 hours later. Inflammatory cells recovered from the air pouches were counted and observed by light microscopy. The cellularity of the implant surfaces was defined by scanning electron microscopy (SEM). In comparison with gold implants, the CH
                        3-coated SAMs recruited a significantly higher number of inflammatory cells. Polymorphonuclear leukocytes (PMN) were more numerous than mononuclear cells (Mo) in the exudates recovered from the air pouches with CH
                        3-coated SAMs. The opposite PMN/Mo proportion was observed in air pouches of the two control groups (mice receiving gold implants or sham-operated animals). A low density of adherent cells was seen on CH 
                        3-coated implants, with no significant quantitative differences during the time course of the study. In contrast, the gold-coated surfaces were covered with numerous cells during all of the 3 days of the inflammation. In conclusion, implants with CH
                        3 surfaces are likely to induce PMN-dominated local acute inflammation but these surfaces are not associated with a significant adherence of leukocytes to the implant. {\textcopyright} 2005 Wiley-Liss, Inc.
},
	keywords = {Air-pouch model, animal experiment, animal model, Animalia, Animals, article, biomaterial, cell adhesion, Cell Count, cell density, cell surface, Cell-surface interactions, Coated Materials, Biocompatible, Contact angle, controlled study, gold, implantation, Implants, Experimental, Inflammation, inflammatory cell, Inflammatory responses, leukocyte adherence, Leukocytes, male, materials testing, methyl group, Mice, Microscopy, Electron, Scanning, mononuclear cell, mouse, neutrophil, nonhuman, priority journal, Scanning electron microscopy, Self-assembled monolayers, Surface Properties, thiol derivative, Time Factors},
	isbn = {1059910X (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-17144372986\&partnerID=40\&md5=1d2d00c4efe22d960872f5e785c7d4c7},
	author = {Barbosa, J.N. and Barbosa, M.A. and {\'A}guas, A.P.}
}
@article {2454,
	title = {Polysaccharides as scaffolds for bone regeneration},
	journal = {ITBM-RBMITBM-RBM},
	volume = {26},
	year = {2005},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: ITBMFdoi: 10.1016/j.rbmret.2005.04.006Language of Original Document: EnglishCorrespondence Address: Barbosa, M.A.; INEB, Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: mbarbosa@ibmc.up.ptChemicals/CAS: alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; arginylglycylaspartic acid, 99896-85-2; cellulose, 61991-22-8, 68073-05-2, 9004-34-6; chitosan, 9012-76-4References: Shogren, R.L., Bagley, E.B., Natural polymers as advanced materials: Some research needs and directions (1999) Biopolymers. Utilizing Nature{\textquoteright}s Advanced Materials, ACS Symposium Series 723, pp. 2-11. , S.H. Iman R.V. Greene B.R. Zaidi Oxford University Press Cary, USA;Kaplan, D.L., Introduction to polymers from renewable resources (1998) Biopolymers from Renewable Resources, pp. 1-29. , D.L. Kaplan Springer Verlag Berlin, Germany; 
Yannas, I.V., Natural materials (1996) Biomaterials Science. An Introduction to Materials in Medicine, pp. 84-94. , B.D. Ratner A.S. Hoffman F.J. Schoen J.E. Lemons Academic Press California, USA; 
Hon, D.-S., Cellulose and its derivatives: Structures, reactions, and medical uses (1996) Polysaccharides in Medicinal Applications, pp. 87-105. , S. Dumitriu Marcel Dekker New York, USA; 
Okajima, K., Role of molecular characteristics on some physiological properties of cellulose derivatives (1989) Cellulose: Structural and Functional Aspects, pp. 439-446. , J.F. Kennedy G.O. Phillips P.A. Williams Ellis Horwood Chichester, UK; 
Ikada, Y., Biomedical applications of cellulose membranes Cellulose: Structural and Functional Aspects, pp. 447-455; 
Miyamoto, T., Takahashi, S., Ito, H., Inagaki, H., Noishiki, Y., Tissue biocompatibility of cellulose and its derivatives (1989) J. Biomed. Mater. Res., 23, pp. 125-133; 
Hayashi, T., Biodegradable polymers for biomedical uses (1994) Prog. Polym. Sci., 19, pp. 663-702; 
Klemm, D., Philipp, B., Heinze, T., Heinze, U., Wagenknecht, W., (1998) Comprehensive Cellulose Chemistry, Vol 1, Fundamentals and Analytical Methods and Functionalization of Cellulose, Vol. 2, , Wiley-VCH Weinheim, Germany; 
Hon, D.-S., Cellulose and its derivatives: Structures, reactions, and medical uses (1996) Polysaccharides in Medicinal Applications, pp. 87-105. , S. Dumitriu Marcel Dekker New York, USA; 
Nevell, T.P., Zeronian, S.H., (1985) Cellulose Chemistry and Its Applications, , Ellis Horwood Chichester, UK; 
Pommier, J.C., Poustis, J., Baquey, C., Chauveaux, D., Biocompatible, hydrophilic material. Method of manufacture and uses of same. Fr Pat 8610331, 1986; Eur Pat 0256906 A1, 1987; US Pat 4904258, 1990Baquey, C., Chauveuax, D., Poustis, J., La cellulose: Un vieux mat́riau pour le developpement de biomat́riaux innovants (1992) Sci. Tech. Technol., 20, pp. 28-32; 
Barb{\'\i}, C., Chauveaux, D., Baquey, C., Poustis, J., Pommier, J.C., Morlier, R., {\'E}valuation des propriet́s mecaniques de mat́riaux cellulosiques et perspectives d{\textquoteright}application originales de ces mat́riaux en chirurgie orthoṕdique (1987) Calcified Tissues and Biomaterials, pp. 319-325. , Proc BIOMAT 87 Congress Bordeaux, France; 
Barb{\'\i}, C., Bareille, R., Harmand, M.-F., Baquey, C., Cellulosic materials for skeletal reparation: Mechanical, physical and biological in vitro behaviour (1989) Calcified Tissues and Biomaterials, , Proc BIOMAT 89 Congress Bordeaux, France; 
Brie, J., Lafage, M.H., Chauveaux, D., Gueroult, J.M., Poustis, J., Baquey, C., La cellulose macroporeuse: Un mat́riau potentiellement utilisable pour le comblement osseux (1992) Biomat́riaux et Organes Artificiels, , Proc BIOMAT 92 Congress Bordeaux, France; 
Poustis, J., Chauveaux, D., Baquey, C., (1992) Dynamic Testing of Cellulose in Orthopaedics Devices and Related Environments, p. 191. , 4th World Biomaterials Congr Berlin, Germany; 
Poustis, J., Baquey, C., Chauveaux, D., Mechanical properties of cellulose in orthopaedic devices and related environments (1994) Clin. Mater., 16, pp. 119-124; 
Chauveaux, D., Gueroult, J.-M., Barb{\'\i}, C., Baquey, C., Cellulosic materials for skeletal reparation: Preliminary study of in vivo biocompatibility (1989) Calcified Tissues and Biomaterials, , Proc BIOMAT 89 Congress Bordeaux, France; 
Barbie, C., Chauveaux, D., Barthe, X., Baquey, C., Poustis, J., Biological behaviour of cellulosic materials after bone implantation: Preliminary results (1990) Clin. Mater., 5, pp. 251-258; 
Gross, U., Muller-Mai, C., Voigt, C., (1992) The Tissue Response on Cellulose Cylinders after Implantation in the Distal Femur of Rabbits, p. 192. , 4th World Biomaterials Congr Berlin, Germany; 
Baquey, C., Barb{\'\i}, C., More, N., Rouais, F., Poustis, J., Chauveaux, D., (1992) In Vivo Study of the Biostability of a Cellulose Material, p. 365. , 4th World Biomaterials Congr Berlin, Germany; 
Mizusawa, Y., Burke, J.R., Prednisolone and cellulose phosphate treatment in idiopathic infantile hypercalcaemia with nephrocalcinosis (1996) J. Pediatr. Child Health, 32, pp. 350-352; 
Parfitt, A.M., Effect of cellulose phosphate on calcium and magnesium homeostasis: Studies in normal subjects and patients with latent hypoparathyroidism (1975) Clin. Sci. Mol. Med., 49, pp. 83-90; 
Pak, C.Y.C., Delea, C.S., Bartter, F.C., Successful treatment of recurrent nephrolithiasis (calcium stones) with cellulose phosphate (1974) N. Engl. J. Med., 290, pp. 175-180; 
Dowd, V., Yon, R.J., Heterogeneous binding of aldolase to phosphocellulose. Interpretation in terms of a "concerted cluster" model of multivalent affinity (1992) J. Chromatogr., 627, pp. 145-151; 
Sugihara, J., Imamura, T., Yanase, T., Separation of peptides by cellulose-phosphate chromatography for identification of a hemoglobin variant (1982) J. Chromatogr., 229, pp. 193-199; 
Granja, P.L., Pouy{\'s}gu, L., Ṕtraud, M., De J́so, B., Baquey, C., Barbosa, M.A., Cellulose phosphates as biomaterials. Synthesis and characterization of highly phosphorylated cellulose gels (2001) J. Appl. Polym. Sci., 82, pp. 3341-3353; 
Granja, P.L., Pouy{\'s}gu, L., Deffieux, D., Daud́, G., De J́so, B., Labrug̀re, C., Cellulose phosphates as biomaterials. Surface chemical modification of regenerated cellulose hydrogels (2001) J. Appl. Polym. Sci., 82, pp. 3354-3365; 
Granja, P.L., Pouy{\'s}gu, L., De J́so, B., Rouais, F., Baquey, C., Barbosa, M.A., Cellulose phosphates as biomaterials. Mineralization of chemically modified regenerated cellulose hydrogels (2001) J. Mater. Sci., 36, pp. 2163-2172; 
Granja, P.L., Ribeiro, C.C., De J́so, B., Baquey, C., Barbosa, M.A., Mineralization of regenerated cellulose hydrogels (2001) J. Mater. Sci. Mater. Med., 12, pp. 785-791; 
Granja, P.L., De J́so, B., Bareille, R., Rouais, F., Baquey, C., Barbosa, M.A., (2004) Cellulose Phosphates As Biomaterials. in Vitro Biocompatibility Studies; 
Granja, P.L., De J́so, B., Bareille, R., Rouais, F., Baquey, C., Barbosa, M.A., (2004) Mineralization of Regenerated Cellulose Hydrogels Induced by Human Osteoprogenitor Cells; 
Fricain, J.C., Granja, P.L., Barbosa, M.A., De J́so, B., Barthe, N., Baquey, C., Cellulose phosphates as biomaterials. in vivo biocompatibility studies (2001) Biomaterials, 23, pp. 971-980; 
Franz, G., Polysaccharides in pharmacy (1986) Adv. Polym. Sci., 76, pp. 1-30; 
Skoog, T., The use of periosteum and surgicel{\textregistered} for bone restoration in congenital clefts of the maxilla (1967) Scand. J. Plast. Reconstr. Surg., 1, pp. 113-130; 
Degenshein, G.A., Hurwitz, A., Ribacoff, S., Experience with regenerated oxidized cellulose (1963) N. Y. State J. Med., 63, pp. 2639-2643; 
Finn, M.D., Schow, S.R., Schneiderman, E.D., Osseous regeneration in the presence of four common hemostatic agents (1992) J. Oral Maxillofac. Surg., 50, pp. 608-612; 
Galgut, P.N., Oxidized cellulose mesh. I. Biodegradable membrane in periodontal surgery (1990) Biomaterials, 11, pp. 561-564; 
Matthew, I.R., Browne, R.M., Frame, J.W., Millar, B.G., Subperiosteal behaviour of alginate and cellulose wound dressing materials (1995) Biomaterials, 16, pp. 275-278; 
Galgut, P.N., Oxidized cellulose mesh. II. Using hydroxy-apatite bone grafting material in the treatment of intrabony deffects (1990) Biomaterials, 11, pp. 565-567; 
De Taillac, L.B., Fricain, J.C., Barthe, N., Brouillaud, B., Labrugere, C., Bareille, R., Validation du concept de materiaux hybrids a base de cellulose macroporeuse - Application a l{\textquoteright}odontologie (2004) Les Cahiers de l{\textquoteright}Adf, 1617, pp. 18-25; 
De Taillac, L.B., (2004), Ph.D. Thesis. University of Bordeaux 1. DecemberMartson, M., Viljanto, J., Hurme, T., Saukko, P., Biocompatibility of cellulose sponge with bone (1998) Eur. Surg. Res., 30, pp. 426-432; 
Martson, M., Viljanto, J., Hurme, T., Laippala, P., Saukko, P., Is cellulose sponge degradable or stable as implantation materials? An in vivo subcutaneous study in the rat (1999) Biomaterials, 20, pp. 1899-1995; 
Pajulo, O., Viljanto, J., Lonnberg, B., Hurme, T., Lonnqvist, K., Saukko, P., Viscose cellulose sponge as an implantable matrix: Changes in the structure increase the production of granulation tissue (1996) J. Biomed. Mater. Res., 32, pp. 439-446; 
De Taillac, L.B., Porte, M.C., Bareille, R., Amedee, J., Labrugere, C., Fortin, F., (2002) Development of a New Generation of Bone Substitute - Covalenet Immobilization of the Active Peptides onto Cellulose, , Society for Biomaterials Conference Florida, USA; 
De Taillac, L.B., Labrugere, C., Amedee, J., Bareille, R., Fricain, J.C., Fortin, F., (2002) Human Osteoblasts Interaction with Adhesion Peptides Grafted onto Cellulose, , 17th European Conference on Biomaterials Barcelona, Spain; 
De Taillac, L.B., Porte-Durrieu, M.C., Labrugere, C., Bareille, R., Aḿd́e, J., Baquey, C., Grafting of RGD peptides to cellulose to enhance human osteoprogenitor cells adhesion and proliferation (2004) Compos. Sci. Technol., 64, pp. 775-915; 
Gombotz, W.R., Wee, S.F., Protein release from alginate matrices (1998) Adv. Drug Deliv. Rev., 31, pp. 267-285; 
Smidsrod, O., Draget, K.I., Chemistry and physical properties of alginates (1996) Carbohydr. Eur., 14, pp. 6-13; 
Eiselt, P., Yeh, J., Latvala, R.K., Shea, L.D., Mooney, D.J., Porous carriers for biomedical applications based on alginate hydrogels (2000) Biomaterials, 21, pp. 1921-1927; 
Alsberg, V., Anderson, K., Albeiruti, A., Franceshi, R.T., Mooney, D.J., Cell interactive alginate hydrogels for bone tissue engineering (2001) J. Dent. Res., 80, pp. 2025-2029; 
Barrias, C.C., Granja, P.L., Meriem, L., S{\'a} Miranda, M.C., Barbosa, M.A., Biological evaluation of calcium alginate microspheres as a vehicle for the localised delivery of a therapeutic enzyme (2005) J. Biomed. Mater Res.; 
Rowley, J.A., Madlambayan, G., Mooney, D.J., Alginate hydrogels as synthetic extracellular matrix materials (1999) Biomaterials, 20, pp. 45-53; 
Dalby, M.J., Di Silvio, L., Harper, E.J., Bonfield, W., Increasing hydroxyapatite incorporation into poly(methylmethacrylate) cement increases osteoblast adhesion and response (2002) Biomaterials, 23, pp. 569-576; 
Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., Calcium phosphate-alginate microspheres as enzyme delivery matrices (2004) Biomaterials, 25, pp. 4363-4373; 
Barrias, C.C., Ribeiro, C.C., S{\'a} Miranda, M.C., Barbosa, M.A., Effect of calcium phosphate addition to alginate microspheres: Modulation of enzyme release kinetics and improvement of osteoblastic cell adhesion (2005) Key Eng. Mater., 284-286, pp. 689-692; 
Khor, E., (2001) Chitin: Fulfilling a Biomaterials Promise. 1st Ed, , Elsevier Oxford, UK; 
Vandevord, P.J., Matthew, H.W.T., Desilva, S.P., Mayton, L., Wu, B., Wooley, P.H., Evaluation of the biocompatibility of a chitosan scaffold in mice (2002) J. Biomed. Mater. Res., 59, pp. 585-590; 
Roberts, G.A.F., (1992) Chitin Chemistry, , The Macmillan Press Houndmills; 
Domard, A., Chitosan interactions Advances in Chitin Science, pp. 410-420. , Domard A, Roberts GAF, Varum KM, editors. Lyon, France: Jacques Andr{\'e} Publisher (1007); 
Tomihata, K., Ikada, Y., In vitro and in vivo degradation of films of chitin and its deacetylated derivatives (1997) Biomaterials, 18, pp. 567-575; 
Muzzarelli, R.A.A., Human enzymatic activities related to the therapeutic administration of chitin derivatives (1997) Cell. Mol. Life Sci., 53, pp. 131-140; 
Muzzarelli, R.A.A., Muzzarelli, C., Natural and artificial chitosan-inorganic composites, a mini-review (2002) Chitosan in Pharmacy and Chemistry: Atec Edizioni, pp. 233-244. , R.A.A. Muzzarelli C. Muzzarelli; 
Lee, J.Y., Nam, S.H., Im, S.Y., Park, Y.J., Lee, Y.M., Seol, Y.J., Chung, C.P., Lee, S.J., Enhanced bone formation by controlled growth factor delivery from chitosan-based biomaterials (2002) J. Control. Release, 78, pp. 187-197; 
Madihally, S.V., Matthew, H.W.T., Porous chitosan scaffolds for tissue engineering (1999) Biomaterials, 20, pp. 1133-1142; 
Nettles, D.L., Elder, S.H., Gilbert, J.A., Potential use of chitosan as a cell scaffold material for cartilage tissue engineering (2002) Tissue Eng., 8, pp. 1009-1016; 
Lahiji, A., Sohrabi, A., Hungerford, D.S., Frondoza, C.G., Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes (2000) J. Biomed. Mater. Res., 51, pp. 586-595; 
Zhao, F., Yin, Y.J., Lu, W.W., Leong, J.C., Zhang, W.J., Zhang, J.Y., Preparation and histological evaluation of biomimetic three-dimensional hydroxyapatite/chitosan-gelatin network composite scaffolds (2002) Biomaterials, 23, pp. 3227-3234; 
Chatelet, C., Damour, O., Domard, A., Influence of the degree of acetylation on some biological properties of chitosan films (2001) Biomaterials, 22, pp. 261-268; 
Dhiman, H.K., Ray, A.R., Panda, A.K., Characterization and evaluation of chitosan matrix for in vitro growth of MCF-7 breast cancer cell lines (2004) Biomaterials, 25, pp. 5147-5154; 
Prasitsilp, M., Jenwithisuk, R., Kongsuwan, K., Damrongchai, N., Watts, P., Cellular responses to chitosan in vitro: The importance of deacetylation (2000) J. Mater. Sci. Mater. Med., 11, pp. 773-778; 
Amaral, I.F., Granja, P.L., Barbosa, M.A., In vitro mineralisation of chitosan membranes carrying phosphate functionalities (2004) Key Eng. Mater., 254-256, pp. 577-580
},
	month = {2005///},
	pages = {212 - 217},
	abstract = {This review is concerned with the use of polysaccharides for bone regeneration. Cellulose, alginate and chitosan have been chosen as examples of the formidable potential of this class of materials. Many others could be cited but our experience and research interests dictated the choice. As in many areas of research we know how they started but we cannot see how and if they will end. Modification of cellulose was our entrance to the world of polysaccharides and a lot of what we know we owe to Charles Baquey, to whom we would like to express our immense gratitude. This chapter is dedicated to him, for his cooperation, unlimited enthusiasm and friendship. {\textcopyright} 2005 Elsevier SAS. All rights reserved.},
	keywords = {Alginate, alginic acid, Amino Acid Sequence, arginylglycylaspartic acid, biomaterial, biotechnology, bone cell, bone prosthesis, Bone Regeneration, Cellulose, chitosan, enzyme degradation, polysaccharide, Polysaccharides, review, Tissue engineering},
	isbn = {12979562 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-20744434609\&partnerID=40\&md5=43d83907f18b3e44035dfe14ff13a24f},
	author = {Barbosa, M.A. and Granja, P.L. and Barrias, C.C. and Amaral, I.F.}
}
@book {2491,
	title = {Mineralised membranes for bone regeneration},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Mesquita, P.; Inst. de Engenharia Biom{\'e}dica, R. do Campo Alegre 823, 4150-180 Porto, PortugalReferences: Baron, M., Haas, R., D{\"o}rtbudak, O., Watzek, G., Experimentally induced peri-implantitis: A review (2000) Int J Oral Maxillofac Implants, 15, pp. 533-544;Hammerle, C.H., Jung, R.E., Feloutzis, A., A systematic review of the survival of implants in bone sites augmented with barrier membranes (guided bone regeneration) in partially edentulous patients (2002) J Clin. Periodontol., 29 (SUPPL. 3), pp. 226-231; 
Brunel, G., Brocard, D., Duffort, J.F., Jacquet, E., Justumus, P., Simonet, T., Benque, E., Bioabsorbable materials for guided bone regeneration prior to implant placement and 7-year followup: Report of 14 cases (2001) J Periodontol., 72 (2), pp. 257-264; 
Karapati, S., Hugson, A., Kugelberg, C.F., Healing following GTR treatment of bone defects distal to mandibular 2 nd molars after removal of impacted 3 rd molars (2000) J. Clin. Periodontol., 27, pp. 325-332; 
Sim{\~o}es, R., Fonseca, L., Vasconcelos, M., Afonso, A., Cavalheiro, J., Modified exoskeleton of shrimp for guided bone regeneration (2002) 17 th ESB, , Barcelona, Spain, September
},
	month = {2004///},
	pages = {1091 - 1094},
	address = {Porto},
	abstract = {Guided bone regeneration can be achieved using filling biomaterials and protective membranes. The aim of this work is to study a new membrane prepared from shrimp shells, without modification of the natural concave shape. The "in vitro" bioactivity was evaluated using a simulated body fluid prepared with substitution of calcium by strontium (SBR-Sr). The bioactivity behaviour was increased using a surface treatment with sodium silicate. The "in vitro" and "in vivo" tests allow to conclude that these membranes are bioactive and can induce bone growth on their surfaces.},
	keywords = {Biological membranes, biomaterial, Biomaterials, Bone, Bone Regeneration, Calcium, Ceramic materials, Deproteinization, Membrane, Mineralised membranes, Natural material, Optical microscopy, Shear strength, Sodium compounds, Strontium, Substitution reactions},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0348145956\&partnerID=40\&md5=51d6fa431f03c745e2def45321a0dfa0},
	author = {Mesquita, P. and Branco, R. and Afonso, A. and Vasconcelos, M. and Cavalheiro, J.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@article {2516,
	title = {Adhesion of human leukocytes to biomaterials: An in vitro study using alkanethiolate monolayers with different chemically functionalized surfaces},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {65},
	year = {2003},
	note = {Cited By (since 1996): 24Export Date: 21 November 2012Source: ScopusCODEN: JBMRCLanguage of Original Document: EnglishCorrespondence Address: Barbosa, J.N.; INEB-Inst. de Engenharia Biomedica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: judite@ibmc.up.ptChemicals/CAS: gold, 7440-57-5; hydroxide, 14280-30-9; methane, 74-82-8; phorbol 12 acetate 13 myristate, 31365-46-5; phytohemagglutinin, 9008-97-3; phorbol 13 acetate 12 myristate, 16561-29-8; Biocompatible Materials; Gold, 7440-57-5; Phytohemagglutinins; Tetradecanoylphorbol Acetate, 16561-29-8References: Jones, D.A., Smith, C.W., McIntire, L.V., Leucocyte adhesion under flow conditions: Principles importante in tissue engineering (1996) Biomaterials, 17, pp. 337-347;Marchant, R.E., Cell adhesion and interactions with biomaterials (1984) J Adhes, 20, pp. 211-225; 
Tegoulia, V.A., Cooper, S.L., Leukocyte adhesion on model surfaces under flow: Effects of surface chemistry, protein adsorption, and shear rate (2000) J Biomed Mater Res, 50, pp. 291-301; 
Brunstedt, M.R., Anderson, J.M., Spilizewski, K.L., Marchant, R.E., Hiltner, A., In vivo leucocyte interactions on Pellethane surfaces (1990) Biomaterials, 11, pp. 370-378; 
Kao, W.J., Evaluation of leukocyte adhesion on polyurethanes: The effects of shear stress and blood proteins (2000) Biomaterials, 21, pp. 2295-2303; 
Lim, F., Cooper, S.L., Effect of sulphonate incorporation on in vitro leucocyte adhesion to polyuretanes (1995) Biomaterials, 16, pp. 457-466; 
Tomczok, J., Sliwa-Tomczok, W., Klein, C.L., Van Kooten, T.G., Kirkpatrick, C.J., Biomaterial-induced alterations of human neutrophils under fluid shear stress: Scanning electron microscopical study in vitro (1996) Biomaterials, 17, pp. 1359-1367; 
Yun, J.K., DeFife, K., Colton, E., Stack, S., Azeez, A., Cahalan, L., Verhoeven, M., Anderson, J.M., Human monocyte/macrophage adhesion and cytokine production on surface-modified poly(tetrafluoroethylene/hexafluoropropylene) polymers with and without protein preadsorption (1995) J Biomed Mater Res, 29, pp. 257-268; 
Mrksich, M., Whitesides, G.M., Interactions of SAMs with proteins (1996) Annu Rev Biophys Biomol Struct, 25, pp. 55-57; 
Ulman, A., Formation and structure of self-assembled monolayers (1996) Chem Rev, 96, pp. 1533-1554; 
Mrkisich, M., Tailored substrates for studies of attached cell culture (1998) Cell Mol Life Sci, 54, pp. 653-662; 
Kane, R.S., Takayama, S., Ostuni, E., Ingber, D.E., Whitesides, G.M., Patterning proteins and cells using soft lithography (1999) Biomaterials, 20, pp. 2363-2376; 
Mrksich, M., Using self-assembled monolayers to understand the biomaterials interface (1997) Curr Opin Colloid Int Sci, 2, pp. 83-88; 
Margel, S., Vogler, E.A., Firment, L., Watt, T., Haynie, S., Sogah, D.Y., Peptide, protein, and cellular interactions with self-assembled monolayer model surfaces (1993) J Biomed Mater Res, 27, pp. 1463-1476; 
Prime, K.L., Whitesides, G.M., Self assembled organic monolayers model systems for studying the adsorption of proteins at surfaces (1991) Science, 252, pp. 1164-1167; 
Lopez, G.P., Albers, M.W., Schreiber, S.L., Carroll, R., Peralta, E., Whitesides, G.M., Convenient methods for patterning the adhesion of mammalian cells to surfaces using self-assembled monolayers of alkane thiolates on gold (1993) J Am Chem Soc, 115, pp. 5877-5878; 
Shinghvi, R., Kumar, A., Lopez, G.P., Stephanopoulos, G.N., Wang, D.I.C., Whitesides, G.M., Ingber, D.E., Engineering cell shape and function (1994) Science, 264, pp. 696-698; 
Pan, S., Castner, D.G., Ratner, B.D., Multitechnique surface characterization of derivatization efficiencies for hydroxyl-terminated self-assembled monolayers (1998) Langmuir, 14, pp. 3545-3550; 
Lin, J., Chuang, W., Synthesis, surface characterization, and platelet reactivity evaluation for the self-assembled monolayer of alkanethiol with sulfonic acid functionality (2000) J Biomed Mater Res, 51, pp. 413-423; 
Lindblad, M., Lestelius, M., Johansson, A., Tengvall, P., Thomsen, P., Cell and soft tissue interactions with methyl- and hydroxyl-terminated alkane thiols on gold surfaces (1997) Biomaterials, 18, pp. 1059-1068; 
Baker, L.C., Davis, W.C., Autieri, J., Watach, M.J., Yamazaki, K., Litwak, P., Wagner, W.R., Flow cytometric assays to detect platelet activation and aggregation in device-implanted calves (1998) J Biomed Mater Res, 41, pp. 312-321; 
English, D., Andersen, B.R., Single-step separation of red blood cells. Granulocytes and mononuclear leukocytes on discontinuous density gradient of ficoll-hypaque (1974) J Immunol Methods, 5, pp. 249-253; 
Nygren, H., Hrustic, E., Karlsson, C., {\"O}ster, L., Respiratory burst response of peritoneal leukocytes adhering to titanium and stainless steel (2001) J Biomed Mater Res, 57, pp. 238-247; 
Gorbet, M.B., Yeo, E.L., Sefton, M.V., Flow cytometric study of in vitro neutrophil activation by biomaterials (1999) J Biomed Mater Res, 44, pp. 289-297; 
K{\"a}lltrorp, M., Askendal, A., Thompsen, P., Tengvall, P., Inflammatory cell recruitment, distribution, and chemiluminescence response at IgG precoated- and thiol functionalized gold surfaces (1999) J Biomed Mater Res, 47, pp. 251-259; 
Mahiout, A., Courtney, J.M., Effect of dialyser membranes on extracellular and intracellular granulocyte and monocyte activation in ex vivo pyrogen-free conditions (1994) Biomaterials, 15, pp. 969-980; 
Davis, R.G., Goodman, S.B., Smith, R.L., Lerman, J.A., Williams III, R.J., The effects of bone cement powder on human adherent monocytes/ macrophages in vitro (1993) J Biomed Mater Res, 27, pp. 1039-1046; 
Gery, I., Gerhon, R.K., Waskman, B., Potentiation of the T lymphocyte response to mitogens. I. The responding cell (1972) J Exp Med, 136, pp. 128-134; 
Peters, S.P., Cerasoli Jr., F., Albertine, K.H., Gee, M.H., Berd, D., Ishihara, Y., "Autoregulation" of human neutrophil activation in vitro: Regulation of phorbol myristate acetate-induced neutrophil activation by cell density (1990) J Leukocyte Biol, 47, pp. 457-474; 
Esaguy, N., {\'A}guas, A.P., Vilanova, M., Silva, M.T., Activation of human neutrophils by phorbol ester decreases the cytoplasm compactness and the lactoferrin content of the granulocytes (1991) J Leukocyte Biol, 50, pp. 444-452; 
Collins, D.P., Cytokine and cytokine receptor expression as a biological indicator of immune activation: Important considerations in the development of in vitro model systems (2000) J Immunol Methods, 243, pp. 125-145; 
Ruoslahti, E., Integrin signaling and matrix assembly (1996) Tumor Biol, 17, pp. 117-128; 
Aoudjit, F., Vuori, K., Engagement of the α2β1 integrin inhibits Fas ligand expression and activation-induced cell death in T cells in a focal adhesion kinase-dependent manner (2000) Blood, 95, pp. 2044-2051; 
Lehr, H.A., Krombach, F., Munzing, S., Bodlaj, R., Glaubitt, S.I., Seiffge, D., Hubner, C., Messmer, K., In vitro effects of oxidized low density lipoprotein on CD11b/CD18 and L-selectin presentation on neutrophils and monocytes with relevance for the in vivo situation (1995) Am J Pathol, 146, pp. 218-227; 
Kansas, G.S., Tedder, T.F., Transmembrane signals generated through MHC class II, CD19, CD20, CD39, and CD40 antigens induce LFA-1-dependent adhesion in human B cells through a tyrosine kinase-dependent pathway (1991) J Immunol, 147, pp. 4094-4102; 
Damle, N.K., Eberhardt, C., Van Der Vieren, M., Direct interactions with primed CD4+ CD45R0+ memory T lymphocytes induces expression of endothelial leukocyte adhesion molecule-1 and vascular cell adhesion molecule-1 on the surface of vascular endothelial cells (1991) Eur J Immunol, 21, pp. 2915-2923; 
Murata, S., Tanaka, T., Miysaka, M., Lymphocyte binding to MAd-CAM-1 via α4β7 integrin activates a signal transduction pathway involving tyrosine phosphorylation of paxillin and p105 Cas-L (2002) Immunol Lett, 81, pp. 223-228; 
Roth, S.J., Carr, M.W., Rose, S.S., Springer, T.A., Characterization of transendothelial chemotaxis of T lymphocytes (1995) J Immunol Methods, 188, pp. 97-116; 
Aziz, K.E., Wakefield, D., In vivo and in vitro expression of adhesion molecules by peripheral blood lymphocytes from patients with primary Sjogren{\textquoteright}s syndrome: Culture-associated enhancement of LECAM-1 and CD4 (1995) Rheumatol Int, 15, pp. 69-74; 
Tengvall, P., Lundstrom, I., Liedberg, B., Protein adsorption studies on model organic surfaces: An ellipsometric and infrared spectroscopic approach (1998) Biomaterials, 19, pp. 407-422; 
Tidwell, C.D., Ertel, S.I., Ratner, B.D., Tarasevich, B.J., Atre, S., Allara, D.L., Endothelial cell growth and protein adsorption on terminally functionalised self-assembled monolayers of alkanethiols on gold (1997) Langmuir, 13, pp. 3404-3413; 
Tsai, M., Lin, J., Surface characterization and platelet adhesion studies of self-assembled monolayer with phosphonate ester and phosphonic acid functionalities (2001) J Biomed Mater Res, 55, pp. 554-565; 
Franco, M., Nealy, P.F., Campbell, S., Teixeira, A.I., Murphy, C.J., Adhesion and proliferation of corneal epithelial cells on self-assembled monolayers (2000) J Biomed Mater Res, 52, pp. 261-269; 
Scotchford, C.A., Gilmore, C.P., Cooper, E., Leggett, G.J., Downes, S., Protein adsorption and human osteoblast-like cell attachment and growth on alkylthiol on gold self-assembled monolayers (2002) J Biomed Mater Res, 59, pp. 84-99; 
Silin, V., Weetall, H., Vanderah, D.J., SPR studies of the non-specific adsorption kinetics of human IgG and BSA on gold surfaces modified by self-assembled monolayers (SAMs) (1997) J Colloid Interface Sci, 185, pp. 94-103
},
	month = {2003///},
	pages = {429 - 434},
	abstract = {The adhesion of human leukocytes to self-assembled monolayers of well-defined surface chemistry was investigated in vitro. Polymorphonuclear (PMN) and mononuclear leukocytes were isolated from human blood by centrifugation techniques. The effect on adhesion of cell activation produced by pre-incubation of leukocytes with phytohemagglutinin (PHA) and phorbol 12-myristate 13-acetate (PMA) was also studied. Gold substrates were modified by treatment with alkanethiols with three different terminal chemical groups: COOH, OH, and CH                        3. After incubation with the two subpopulations of leukocytes, the monolayers were washed, treated with fixative, stained with a Giemsa method, and observed by light microscopy to quantify the number of attached leukocytes. Comparative quantification of the density of leukocyte adhesion to the three types of self-assembled monolayers was determined. The hydrophobic surface expressing CH
                        3 was found to be the one that induced the highest adhesion density of leukocytes, both of PMN and mononuclear cells. In vitro activation of both mononuclear and PMN leukocytes further increased cell adhesion to the chemically defined monolayers that were used. This enhancement was higher for PHA-activated than for PMA-stimulated mononuclear cells, whereas PMA treatment of neutrophils resulted in a higher rate of adhesion of these cells than PHA stimulation. {\textcopyright} 2003 Wiley Periodicals, Inc.
},
	keywords = {alkane derivative, article, Biocompatible Materials, biomaterial, Biomaterials, Blood, carboxyl group, cell adhesion, cell interaction, cell isolation, cell subpopulation, cell surface, Cell-surface interactions, Cells, Centrifugation, controlled study, drug effect, Giemsa stain, gold, human, human cell, Humans, hydrophobicity, hydroxide, in vitro study, Incubation, lectin, Lectins, leukocyte, Leukocyte activation, leukocyte adherence, Leukocyte adhesion, Leukocytes, Leukocytes, Mononuclear, materials testing, methane, microscopy, Monolayers, mononuclear cell, neutrophil, Neutrophils, Optical microscopy, phorbol 12 acetate 13 myristate, phorbol 13 acetate 12 myristate, physiology, phytohemagglutinin, Phytohemagglutinins, polymorphonuclear cell, Self-assembled monolayers, Surface Properties, surface property, Tetradecanoylphorbol Acetate, thiolacid},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0042970748\&partnerID=40\&md5=87f1101c5250d34ddc259f7f43f37e16},
	author = {Barbosa, J.N. and Barbosa, M.A. and {\'A}gua, A.P.}
}
@article {2512,
	title = {Albumin adsorption on alkanethiols self-assembled monolayers on gold electrodes studied by chronopotentiometry},
	journal = {BiomaterialsBiomaterials},
	volume = {24},
	year = {2003},
	note = {Cited By (since 1996): 31Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(03)00244-8PubMed ID: 12818541Language of Original Document: EnglishCorrespondence Address: Martins, M.C.L.; INEB - Inst. de Engenharia Biomed., Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: cmartins@ibmc.up.ptChemicals/CAS: gold, 7440-57-5; thiol derivative, 13940-21-1; water, 7732-18-5; Albumins; Alkanes; Biocompatible Materials; Gold, 7440-57-5; Serum Albumin; Sulfhydryl CompoundsReferences: Ratner, B.D., Hoffman, A.S., Schoen, F.J., Lemons, J.L., (1996) Biomaterials science - an introduction to materials in medicine, , San Diego, CA, USA: Academic Press;Nakanishi, K., Sakiyama, T., Imamura, K., On the adsorption of proteins on solid surfaces, a common but very complicated phenomenon (2001) J Biosci Bioeng, 91, pp. 233-244; 
Mrksich, M., Whitesides, G.M., Using self-assembled monolayers to understand the interactions of man-made surfaces with proteins and cells (1996) Annu Rev Biophys Biomol Struct, 25, pp. 55-78; 
Sigal, G.B., Mrksich, M., Whitesides, G.M., Effect of surface wettability on the adsorption of proteins and detergents (1998) J Am Chem Soc, 120, pp. 3464-3473; 
Taborelli, M., Eng, L., Descouts, P., Ranieri, J.P., Bellamkonda, R., Aebisher, P., Bovine serum albumin conformation on methyl and amine functionalized surfaces compared by scanning force microscopy (1995) J Biomed Mater Res, 29, pp. 707-714; 
Silin, V., Weetall, H., Vanderah, D., SPR studies of the nonspecific adsorption kinetics of human IgG and BSA on gold surfaces modified by self-assembled monolayers (SAMs) (1997) J Colloid Interface Sci, 185, pp. 94-103; 
Margel, S., Vogler, E.A., Firment, L., Watt, T., Haynie, S., Sogah, D.Y., Peptide, protein, and cellular interactions with self-assembled monolayer model surfaces (1993) J Biomed Mater Res, 27, pp. 1463-1476; 
Wadu-Mesthrige, K., Amro, N.A., Liu, G., Immobilization of proteins on self-assembled monolayers (2000) Scanning, 22, pp. 380-388; 
Takahara, A., Hara, Y., Kojio, K., Kajiyama, T., Plasma protein adsorption behaviour onto the surface of phase-separated organosilane monolayers on the basis of scanning force microscopy (2002) Colloids Surf B: Biointerfaces, 23, pp. 141-152; 
Kalltorp, M., Carl{\'e}n, A., Thomsen, P., Olsson, J., Tengvall, P., Analysis of rat plasma proteins desorbed from gold and methyl- and hydroxyl-terminated alkane thiols on gold surfaces (2000) J Mater Sci: Mater Med, 11, pp. 191-199; 
Tidwell, C.D., Ertel, S.I., Ratner, B.D., Endothelial cell growth and protein adsorption on terminal functionalized, self-assembled monolayers of alkanethiolates on gold (1997) Langmuir, 13, pp. 3404-3413; 
Bain, C.D., Troughton, E.B., Tao, Y.T., Evall, J., Whitesides, G.M., Nuzzo, R.G., Formation of monolayer films by the spontaneous assembly of organic thiols from solution onto gold (1989) J Am Chem Soc, 111, pp. 321-335; 
Ulman, A., (1991) An introduction to ultrathin organic films: From Langmuir-Blodgett to self-assembled, , San Diego, CA, USA: Academic Press; 
Lin, J., Chuang, W., Synthesis, surface characterization, and platelet reactivity evaluation for the self-assembled monolayer of alkanethiol with sulfonic acid functionlity (2000) J Biomed Mater Res, 51, pp. 413-423; 
Mirsky, V.M., Riepl, M., Wolfbeis, O.S., Capacitive monitoring of protein immobilization and antigen/antibody reactions on monomolecular alkylthiol films on gold electrodes (1997) Biosensors Bioelectron, 12, pp. 977-989; 
Viana, A.S., Jones, A.H., Abrantes, L.M., Kalaji, M., Redox induced orientational changes in a series of short chain ferrocenyl alkyl thiols self-assembled on gold(1 1 1) electrodes (2001) J Electroanal Chem, 500, pp. 290-298; 
Kondo, T., Okamura, M., Uosaki, K., Anion effect on the electrochemical characteristics of a gold electrode modified with a self-assembled monolayer of ferrocenylhexanethiol in aqueous and dichlomethane solutions (2001) J Organometal Chem, 637-639, pp. 841-844; 
Dong, S., Li, J., Self-assembled monolayers of thiols on gold electrodes for bioelectrochemistry and biosensors (1997) Bioelectrochem Bioenergetics, 42, pp. 7-14; 
Gooding, J.J., Hibbert, D.B., The application of alkanethiol self-assembled monolayers to enzyme electrodes (1999) Trends Anal Chem, 18, pp. 525-533; 
Spangler, B.D., Tyler, B.J., Capture agents for quartz crystal microbalance-continuous flow biosensor: Functionalized self-assembled monolayers on gold (1999) Anal Chem Acta, 399, pp. 51-62; 
Tegoulia, V.A., Cooper, S.L., Leukocyte adhesion on model surfaces under flow: Effects of surface chemistry, protein adsorption and shear rate (2000) J Biomed Mater Res, 50, pp. 291-301; 
Tengvall, P., Lundstrom, I., Liedberg, B., Protein adsorption studies on model organic surfaces: An ellipsometric and infrared spectroscopic approach (1998) Biomaterials, 19, pp. 407-422; 
Shen, D., Huang, M., Chow, L., Yang, M., Kinetic profile of the adsorption and conformational change of lysozyme on self-assembled monolayers as revealed by quartz crystal resonator (2001) Sensor Actuators B, 77, pp. 664-670; 
Gehanno, V., Freitas, P.P., Veloso, A., Ferreira, J., Almeida, B., Sousa, J.B., Kling, A., Silva, M.F., Ion beam deposition of Mn-Ir spin valves (1999) IEEE Trans Magn, 35, pp. 4361-4367; 
Horbett, T.A., Techniques for protein adsorption studies (1986) Techniques of biocompatibility tests, 2, pp. 183-214. , Williams D.F. Harcover: CRC Press; 
Du, Y.J., Cornelius, R.M., Brash, J.L., Measurement of protein adsorption to gold surface by radioiodination methods: Suppression of free iodide sorption (2000) Colloid Surf B, 17, pp. 59-67; 
Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Klenk, D.C., Measurement of protein using bicinchoninic acid (1985) Anal Biochem, 150, pp. 756-785; 
Lima, J., Sousa, S.R., Ferreira, A., Barbosa, M.A., Interactions between calcium, phosphate, and albumin on the surface of titanium (2001) J Biomed Mater Res, 55, pp. 45-53; 
(1993) Iodine-125. A Guide to Radioiodination Techniques, p. 64. , Amersham Life Science. Little Chalfort, UK: Amersham International plc; 
Bain, C.D., Evall, J., Whitesides, G.M., Formation of monolayer coadsorption of thiols on gold: Variation in the head group, tail group and solvent (1989) J Am Chem Soc, 111, pp. 7155-7164; 
Castner, D.G., Hinds, K., Grainger, D.W., X-ray photoelectron spectroscopy sulfur 2p study of organic thiol and disulfide binding interactions with gold surfaces (1996) Langmuir, 12, pp. 5083-5086; 
Keller, H., Schrepp, W., Fuchs, H., Self-assembled organic films on gold and silver (1992) Thin Solid Films, 210-211, pp. 799-802; 
Chidsey, C.E.D., Loiacono, D.N., Chemical functionality in self-assembled monolayers: Structural and electrochemical properties (1990) Langmuir, 6, pp. 682-691; 
Porter, M.D., Bright, T.B., Allara, D.L., Chidsey, C.E.D., Spontaneously organized molecular assemblies. 4. Structural characterization of n-alkyl thiol monolayers on gold by optical ellipsometry, infrared spectroscopy and electrochemistry (1987) J Am Chem Soc, 109, pp. 3559-3568; 
Miller, C., Cuendet, P., Gratzel, M., Adsorbed ω-hydroxy thiol monolayers on gold electrodes: Evidence for electron tunneling to redox species in solution (1991) J Phys Chem, 95, pp. 877-886; 
Krysinski, P., Brzostowska-Smolska, M., Capacitance characteristics of self-assembled monolayers on gold electrode (1998) Bioelectrochem Bioenergetics, 44, pp. 163-168; 
Guo, B., Anzai, J., OSA, T., Adsorption behaviour of serum albumin on electrodes surfaces and the effects of electrode potential (1996) Chem Pharm Bull, 44, pp. 800-803; 
Dent, A.H., Aslam, M., Other categories of protein coupling (1998) Bioconjugation, protein coupling techniques for the biomedical sciences, pp. 504-569. , M. Aslam, \& A.H. Dent. London: Macmillan; 
Seigal, R.R., Harder, P., Dahint, R., Grunze, M., On-line detection of nonspecific protein adsorption at artificial surfaces (1997) Anal Chem, 69, pp. 3321-3328; 
Norde, W., Giacomelli, C.E., BSA structural changes during homomolecular exchange between the adsorbed and the dissolved states (2000) J Biotechnol, 79, pp. 259-268; 
Peters T., Jr., (1996) All about albumin: Biochemistry, genetics, and medical applications, , San Diego, CA, USA: Academic Press; 
Feng, L., Andrade, J.D., Protein adsorption on low temperature isotropic carbon. III. Isoterms, competitivity, desorption and exchange of albumin and fibrinogen (1994) Biomaterials, 15, pp. 323-333
},
	month = {2003///},
	pages = {3697 - 3706},
	abstract = {Chronopotentiometry was used to study the adsorption of human serum albumin (HSA) to self-assembled monolayers with the following terminal functional groups: CH                        3, COOH and OH. Surfaces were characterized by X-ray photoelectron spectroscopy, water contact angle measurements and cyclic voltammetry. HSA coverage of the different SAMs was investigated by chronopotentiometry and the total amount of adsorbed protein was determined using radiolabelled albumin. Both techniques have demonstrated that HSA adsorption to the different SAM-modified electrodes increases in the following order: OH\&lt;COOH\&lt;CH
                        3-terminated SAMs. A good correlation between coverage and total amount of HSA adsorbed was observed for long adsorption times (900s). {\textcopyright} 2003 Elsevier Science Ltd. All rights reserved.
},
	keywords = {Adsorption, Albumin, Albumin adsorption, Albumins, alkane, Alkanes, article, Biocompatible Materials, biomaterial, Biomaterials, carboxyl group, Chemical modification, Chronopotentiometry, Contact angle, correlation analysis, cyclic potentiometry, Cyclic voltammetry, electrochemistry, electrode, Electron Probe Microanalysis, Functional groups, gold, Humans, hydroxyl group, isotope labeling, methyl group, Monolayers, potentiometry, priority journal, protein analysis, Self assembly, Self-assembled monolayers, Serum Albumin, Sulfhydryl Compounds, Surface modification, Surface Properties, surface property, temperature, thiol derivative, Time Factors, Water, X ray photoelectron spectroscopy},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0038802804\&partnerID=40\&md5=d9b75bc199d3c909e08c53f4de329ce5},
	author = {Martins, M.C.L. and Fonseca, C. and Barbosa, M.A. and Ratner, B.D.}
}
@article {2508,
	title = {Constructing thromboresistant surface on biomedical stainless steel via layer-by-layer deposition anticoagulant},
	journal = {BiomaterialsBiomaterials},
	volume = {24},
	year = {2003},
	note = {Cited By (since 1996): 67Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(03)00363-6PubMed ID: 12951013Language of Original Document: EnglishCorrespondence Address: Ji, J.; Department of Polymer Science, Zhejiang University, Hangzhou 310027, China; email: jianji@mail.hz.zj.cnChemicals/CAS: heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; polyethyleneimine, 74913-72-7; stainless steel, 12597-68-1; trometamol, 1185-53-1, 77-86-1; Anticoagulants; Coated Materials, Biocompatible; Heparin, 9005-49-6; Polyethyleneimine, 9002-98-6; Stainless Steel, 12597-68-1Manufacturers: AldrichReferences: Bertrand, P., Jonas, A., Laschewsky, A., Legras, R., Ultrathin polymer coating by complexation of polyelectrolytes at interfaces: Suitable materials, structure and properties (2000) Macromol Rapid Commun, 21, pp. 319-348;Ulman, A., Formation and structure of self-assembled monolayers (1996) Chem Rev, 96, pp. 1533-1554; 
Sano, M., Lvov, Y., Kunitake, T., (1996) Annu Rev Mater Sci, 26, pp. 153-187; 
Laschewsky, A., (1997) Eur Chem Chron ECC, 2, pp. 13-24; 
Picart, C., Lavalle, Ph., Hubert, P., Cuisnier, Fj., Decher, G., Schaaf, P., Voegel, J.C., Buildup mechanism for poly (L-lysine)/hyaluronic acid films onto a solid surface (2001) Langmuir, 17, pp. 7414-7424; 
Houska, M., Brynda, E., Interactions of proteins with polyelectrolytes at solid/liquid interfaces: Sequential adsorption of albumin and heparin (1997) J Colloid Interface Sci, 188, pp. 243-250; 
Brynda, E., Houska, M., Multiple alternating molecular layers of albumin and heparin on solid surfaces (1996) J Colloid Interface Sci, 183, pp. 18-25; 
Thomann, U.I., Uggowitzer, P.J., Wear-corrosion behavior of biocompatible austenitic stainless steels (2000) Wear, 239, pp. 48-58; 
Cheatham, J., Improved stents for pediatric applications (2001) Prog Pediatr Cardiol, 14, pp. 95-115; 
Donachie, M., Biomedical alloys (1998) Adv Mater Process, 154, pp. 63-65; 
Decher, G., Fuzzy nanoassemblies: Toward layered polymeric multicomposites (1997) Science, 277, pp. 1232-1237; 
Raposo, M., Pontes, R.S., Mattoso, L.H.C., Oliveira, O.N., Kinetics of adsorption of poly (o-methoxyaniline) self-assembled films (1997) Macromolecules, 30, pp. 6095-6101; 
Vov, Y.L., Rusling, J., Thomsen, D.L., Papadimitrakopoulos, F., Kawakami, T., Kunitake, T., High-speed multilayer film assembly by alternate adsorption of silica nanoparticles and linear polycation (1998) Chem Commun, 11, pp. 1229-1230; 
Lowack, K., Helm, C.A., Controlling the self-assembly process of polyelectrolyte multilayers (1998) Macromolecules, 31, pp. 823-833; 
Cheng, Z., Cheng, L., Dong, S., Yang, X., Formation and characterization of heteropolyacid/polycation multilayer films on gold electrode (2001) J Electrochem Soc, 148, p. 227
},
	month = {2003///},
	pages = {4699 - 4705},
	abstract = {Multilayer films consisting of polyethylenimine (PEI) and heparin were successfully prepared on biomedical 316L stainless steel surface via electrostatic self-assembly (ESA) of the PEI and heparin. The process of ESA of PEI/heparin was monitored by static contact angle, electrochemical impedance spectroscopy (EIS), reflection adsorption spectroscopy and X-ray photoelectron spectroscopy data. The contact angle and EIS data revealed that the multilayer coating was stable in Tris-HCl (pH 7.35) buffer solution for 21 days. The static platelet adhesion and static clotting time experiments indicated that the PEI/heparin-deposited stainless steel could resist the platelet adhesion and prolong the static clotting time effectively. Such an easy processing and shape-independent method may have good potential for surface modification of cardiovascular devices. {\textcopyright} 2003 Elsevier Ltd. All rights reserved.},
	keywords = {316L stainless steel, Adhesion, Adsorption, anticoagulant agent, Anticoagulants, aqueous solution, article, Biocompatibility, biomaterial, Biomaterials, blood clotting time, Blood Platelets, Blood-compatibility, buffer, Coated Materials, Biocompatible, coating, Coatings, Contact angle, controlled study, Deposition, device, electricity, Electrochemical impedance spectroscopy, electrochemistry, Electrostatics, heparin, human, human cell, Humans, implant, Layer-by-layer, material coating, materials testing, Multilayers, Organic polymers, pH, Platelet Adhesiveness, Polyethyleneimine, priority journal, reflection adsorption spectroscopy, Self assembly, Spectroscopy, Stainless steel, Surface Properties, surface property, Surfaces, thrombocyte adhesion, Thromboresistant surfaces, trometamol, X ray photoelectron spectroscopy},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0041386468\&partnerID=40\&md5=a68c77a7525bca1760cc298a394c713f},
	author = {Tan, Q. and Ji, J. and Barbosa, M.A. and Fonseca, C. and Shen, J.}
}
@article {2520,
	title = {Surface engineering of poly(DL-lactide) via electrostatic self-assembly of extracellular matrix-like molecules},
	journal = {BiomacromoleculesBiomacromolecules},
	volume = {4},
	year = {2003},
	note = {Cited By (since 1996): 48Export Date: 21 November 2012Source: ScopusCODEN: BOMAFdoi: 10.1021/bm025773pPubMed ID: 12625735Language of Original Document: EnglishCorrespondence Address: Ji, J.; Department of Polymer Science/Eng., Zhejing University, Hangzhou 310027, China; email: jianji@mail.hz.zj.cnChemicals/CAS: gelatin, 9000-70-8; polyethyleneimine, 74913-72-7; polylactide, 26680-10-4; poly(lactide), 26969-66-4; PolyestersTradenames: AXIOVERT 200, Zeiss; ESCA LAB 200A, VG; Radiance 2100, Biorad; Shimadzu UV-1201, Shimadzu; SPA 400, Seiko; Vectro 22, BrukerManufacturers: Biorad; Bruker; Seiko; Shimadzu; VG; ZeissReferences: Sittinger, M., Perka, C., Schutz, O., Haupl, T., Burmester, G.R.Z., (1999) Rheumatol., 58, pp. 130-135;Kim, B.S., Mooney, D.J., (1998) Trends Biotechnol., 16, p. 224; 
Bertrand, P., Jonas, A., Laschewsky, A., Legras, R., (2000) Macromol. Rapid Commun., 21, p. 319; 
Ulman, A., (1996) Chem. Rev., 96, p. 1533; 
Sano, M., Lvov, Y., Kunitake, T., (1996) Annu. Rev. Mater. Sci., 26, pp. 153-187; 
Laschewsky, A., (1997) Eur. Chem. Chronicle ECC, 2, pp. 13-24; 
Chluba, J., Voegel, J.C., Decher, G., Erbacher, P., Schaaf, P., Ogier, J., (2001) Biomacromolecules., 2, pp. 800-805; 
Iler, R., (1966) J. Colloid Interface Sci., 21, p. 569; 
Fromhertz, P., (1980) Electron Microscopy at Molecular Dimension, p. 338. , Baumeister, W., Ed.; Springer-Verlag: Berlin; 
Lee, H., Kepley, L., Hong, H.-G., Mallouk, T., (1988) J. Am. Chem. Soc., 110, p. 618; 
Decher, G., Hong, J.-D., (1991) Ber. Bunsen-Ges., 95, p. 1430; 
Decher, G., (1997) Science, 227, p. 1232; 
Lvov, Y., Decher, G., Mohwald, H., (1993) Langmuir, 9, p. 481; 
Lvov, Y., Haas, H., Decher, G., Mohwald, H., Mikhailov, A., Mtchedlishvily, B., Morgunova, E., Vainshtein, B., (1994) Langmuir, 10, p. 4232; 
Kleinfeld, E., Ferguson, G., (1994) Science, 265, p. 370; 
Hong, J.-D., Lowack, K., Schmitt, J., Decher, G., (1993) Prog. Colloid Polym. Sci., 93, p. 98; 
Schmitt, J., Grunewald, T., Krajer, K., Pershan, P., Decher, G., Loshe, M., (1993) Macromolecules, 26, p. 7058; 
Korneev, D., Lvov, Y., Decher, G., Schmitt, J., Yarodaikin, S., (1995) Physica B, 214, p. 954; 
Iodine-125, a Guide to Radioiodination Techniques (1993), p. 64. , Amersham Life ScienceHwan, M.K., Sang, B.H., Moon, S.K., Jong, S.K., Goo, T.O., Dong, H.H., (1996) J. Pharmacol. Toxicol. Methods, 36, pp. 163-169; 
Adamson, A.W., Gast, A.P., (1997) Physical Chemsitry of Surfaces, pp. 27-28. , John Wiley \& Sons: New York; 
Klagsbrum, M., (1979) Methods Enzymol., 58, p. 560; 
Blum, R.N., Wright, R.A., (1992) J. Gen. Intern. Med., 7, pp. 140-144; 
Okuda, M., Miura, I., Juji, F., Takashima, H., (1994) Int. Arch. Allergy Immunol., 104 (1 SUPPL. 1), pp. 6-8; 
Mosmann, T., (1983) J. Immunol. Methods, 65, pp. 55-63; 
Zund, G., Ye, Q., Hoerstrup, S.P., Schoeberlein, A., Schmid, A.C., Grunenfelder, J., Vogt, P., Turina, M., (1999) Eur. J. Cardio-thoracic Surg., 15, pp. 519-524; 
Risbud, M.V., Bhonde, M.R., Bhonde, R.R., (2001) J. Biomed. Mater. Res., 57, pp. 300-305; 
Strand, B.L., Ryan, T.L., In{\textquoteright}t Veld, P., Kulseng, B., Rokstad, A.M., Skjak-Brek, G., Espevik, T., (2001) Cell Transplant., 10, pp. 263-275; 
De Ceuninck, F., Gaufillier, S., Bonnaud, A., Sabatini, M., Lesur, C., Pastoureau, P., (2001) Biochem. Biophys. Res. Commun., 285, pp. 926-931; 
Bancel, S., Hu, W.S., (1996) Biotechnol. Prog., 12, pp. 398-402; 
Davids, S., (1993) Test Procedures for the Blood Compatibility of Biomaterials, pp. 287-330. , Kluwer Academic Publishers: Dordrecht
},
	month = {2003///},
	pages = {378 - 386},
	abstract = {We report the development of new biomacromolecule coatings on biodegradable biomaterials based on electrostatic assembly of extracellular matrix-like molecules. Poly(ethylene imine) (PEI) was employed to engineer poly(DL-lactide) (PDL-LA) substrate to obtain a stable positively charged surface. An extracellular matrix- (ECM-) like biomacromolecule, gelatin, was selected as the polyelectrolyte to deposit on the activated PDL-LA substrate via the electrostatic assemble technique. The extracellular matrix-like multilayer on the PDL-LA substrate was investigated by attenuated total reflection (ATR-FTIR), X-ray photoelectron spectrscopy (XPS), contact angle, and atomic force microscopy (AFM). The gradual buildup of the protein layer was investigated by UV-vis spectra, and it was further given a quantitative analysis of the protein layer on the PDL-LA substrate via the radioiodination technique. The stability of the protein layer under aqueous condition was also tested by the radiolabeling method. Chondrocyte was selected as the model system for testing the cell behavior and morphology on modified PDL-LA substrates. The chondrocyte test about cell attachment, proliferation, cell activity and cell morphology by SEM, and confocal laser scanning microscopy (CLSM) investigation on extracellular matrix-like multilayer modified PDL-LA substrate was shown to promote chondrocyte attachment and growth. Comparing conventional coating methods, polyelectrolyte multiplayers are easy and stable to prepare. It may be a good choice for the modification of 3-D scaffolds used in tissue engineering. These very flexible systems allow broad medical applications for drug delivery and tissue engineering.},
	keywords = {animal cell, Animalia, Animals, article, Atomic force microscopy, biodegradability, biomaterial, cartilage cell, cell activity, cell adhesion, cell function, cell growth, cell proliferation, cell structure, Cells, Cultured, Chondrocytes, confocal laser microscopy, controlled study, Electron Probe Microanalysis, Electrostatics, extracellular matrix, Fourier transform infrared spectroscopy, gelatin, macromolecule, Macromolecules, Microscopy, Atomic Force, nonhuman, polyelectrolyte, Polyesters, Polyethyleneimine, Polyethylenes, polylactide, priority journal, protein analysis, protein stability, quantitative analysis, Rabbits, radioiodination, Scanning electron microscopy, Self assembly, Spectrophotometry, Ultraviolet, Spectroscopy, Fourier Transform Infrared, Surface charge, Surface engineering, Surface Properties, Tissue engineering, Ultraviolet spectroscopy, X ray photoelectron spectroscopy},
	isbn = {15257797 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0038071804\&partnerID=40\&md5=dc56622e3535f161005efe1ae86f4d23},
	author = {Zhu, H. and Ji, J. and Tan, Q. and Barbosa, M.A. and Shen, J.}
}
@article {2533,
	title = {Cellulose phosphates as biomaterials. In vivo biocompatibility studies},
	journal = {BiomaterialsBiomaterials},
	volume = {23},
	year = {2002},
	note = {Cited By (since 1996): 45Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(01)00152-1PubMed ID: 11791931Language of Original Document: EnglishCorrespondence Address: Granja, P.L.; Laborat{\'o}rio de Biomateriais, INEB-Inst. de Engenliaria Biomedica, Rua do Campo Alegre, 823-4150-180 Porto, Portugal; email: pgranja@ibmc.up.ptChemicals/CAS: Biocompatible Materials; Calcium Radioisotopes; Calcium, 7440-70-2; Cellulose, 9004-34-6; phosphocellulose, 9015-14-9References: Jochanan, H., Boss, M.D., Osseointegration (1999) J Long-Term Eff Med Impl, 9, pp. 1-10;Albrektsson, T., Hard tissue response (1998) Handbook of biomaterial properties, pp. 500-512. , Black J, Hastings G, editors. London, UK: Chapman \& Hall; 
Hench, L.L., Bioactive materials: The potential for tissue regeneration (1998) J Biomed Mater Res, 41, pp. 511-517; 
Yaszemski, M.J., Payne, R.G., Hayes, W.C., Langer, R., Mikos, A.G., Evolution of bone transplantation: Molecular, cellular and tissue strategies to engineer human bone (1996) Biomaterials, 17, pp. 175-185; 
Hollinger, J., McAllister, B., Bone and its repair (1995) Bioceramics, 8, pp. 3-9. , Wilson J, Hench LL, Greenspon D, editors. FL, USA: Pergamon Press; 
Friedman, R.J., Black, J., Galante, J.O., Jacobs, J.J., Skinner, H.B., Current concepts in orthopaedic biomaterials and implant fixation (1993) J Bone Jt Surg, 75 A, pp. 1086-1109; 
Bakker, D., Grote, J.J., Vrouenraets, C.M.F., Hesseling, S.C., De Wijn, J.R., Van Blitterswijk, C.A., Bone-bonding polymer (polyactive{\texttrademark}) (1990) Clinical implant materials, Advances in Biomaterials Series, 9, pp. 99-104. , Heimke G, Solt{\'e}sz U, Lee AJC, editors. Amsterdam, The Netherlands: Elsevier; 
LeGeros, R.Z., Calcium phosphate materials in restorative dentistry: A review (1988) Adv Dent Res, 2, pp. 164-183; 
Hastings, G.W., Mahmud, F.A., Intelligent orthopaedic materials (1993) J Intell Mat Syst Str, 4, pp. 452-456; 
Black, J., (1988) Orthopaedic biomaterials in research and practice, , New York, USA: Churchill Livingstone; 
Li, P., Ye, X., Kangasniemi, I., De Blieck-Hogervorst, J.M.A., Klein, C.P.A.T., De Groot, K., In vivo calcium phosphate formation induced by sol-gel-prepared silica (1995) J Biomed Mater Res, 29, pp. 325-328; 
Tanahashi, M., Yao, T., Kokubo, T., Minoda, M., Miyamoto, T., Nakamura, T., Yamamuro, T., Apatite coated on organic polymers by biomimetic process: Improvement in its adhesion to substrate by glow-discharge treatment (1995) J Biomed Mater Res, 29, pp. 349-357; 
Li, P., Ohtsuki, T., Kokubo, T., Nakanishi, K., Soga, N., Nakamura, T., Yamamuro, T., Process of formation of bone-like apatite layer on silica gel (1993) J Mater Sci: Mater Med, 4, pp. 127-131; 
Radin, S.R., Ducheyne, P., The effect of calcium phosphate ceramic composition and structure on in vitro behavior. II. Precipitation (1993) J Biomed Mater Res, 27, pp. 35-45; 
Puleo, D.A., Nanci, A., Understanding and controlling the bone-implant interface (1999) Biomaterials, 20, pp. 2311-2321; 
McFarland, C.D., Mayer, S., Scotchford, C., Dalton, B.A., Steele, J.G., Downes, S., Attachment of cultured human bone cells to novel polymers (1999) J Biomed Mater Res, 44, pp. 1-11; 
Dalton, B.A., McFarland, C.D., Gengenbach, T.R., Griesser, H.J., Steele, J.G., Polymer surface chemistry and bone cell migration (1998) J Biomater Sci Polym Ed, 9, pp. 781-799; 
Scotchford, C.A., Cooper, E., Leggett, G.J., Downes, S., Growth of human osteoblast-like cells on alkenethiol on gold self-assembled monolayers: The effect of surface chemistry (1998) J Biomed Mater Res, 41, pp. 431-442; 
Adams, A.M., Soames, J.V., Searle, R.F., Ultrastructural changes in cultured human periodontal ligaments cells exposed to dental materials (1995) J Biomed Mater Res, 29, pp. 999-1004; 
Lamba, N.M.K., Baumgartner, J.A., Cooper, S.L., Cell-synthetic surface interactions (1998) Frontiers in tissue engineering, pp. 121-137. , Patrick CW, Mikos AG, McIntire LV, editors. Amsterdam, The Netherlands: Elsevier; 
Dalton, B.A., McFarland, C.D., Gengenbach, T.R., Griesser, H.J., Steele, J.G., Polymer surface chemistry and bone cell migration (1998) J Biomater Sci Polym Ed, 9, pp. 781-799; 
Healy, K.E., Thomas, C.H., Rezania, A., Kim, J.E., McKeown, P.J., Lom, B., Hockberger, P.E., Kinetics of bone cell organization and mineralization on materials with patterned surface chemistry (1996) Biomaterials, 17, pp. 195-208; 
Kasemo, B., Lausmaa, J., Material-tissue interfaces: The role of surface properties and processes (1995) Environ Health Perspect, 102, pp. 41-45; 
Von Recum, A.F., Van Kooten, T.G., The influence of micro-topography on cellular response and the implications for silicone implants (1995) J Biomater Sci Polym Ed, 7, pp. 181-198; 
Vogler, E.A., (1993) Interfacial chemistry in biomaterials science, Surfactant Science Series, vol. 49 (Wettability), 49, pp. 183-250. , Berg JC, editor. New York, USA: Marcel Dekker; 
Horbett, T.A., Weathersby, P.K., Adsorption of proteins from plasma to a series of hydrophilic-hydrophobic copolymers. I. Analysis with the in situ radionation technique (1981) J Biomed Mater Res, 15, pp. 403-423; 
Martson, M., Viljanto, J., Hurme, T., Saukko, P., Biocompatibility of cellulose sponge with bone (1998) Eur Surg Res, 30, pp. 426-432; 
Gross, U., Muller-Mai, C., Voigt, C., The tissue response on cellulose cylinders after implantation in the distal femur of rabbits (1992) Fourth World Biomaterials Congress, p. 192. , Berlin, Germany, April; 
Chauveaux, D., Barbie, C., Barthe, X., Baquey, C., Poustis, J., Biological behaviour of cellulosic materials after bone implantation: Preliminary results (1990) Clin Mater, 5, pp. 251-258; 
Ikada, Y., Biomedical applications of cellulose membranes (1989) Cellulose: structural and functional aspects, pp. 447-455. , Kennedy JF, Phillips GO, Williams PA, editors. Chichester, UK: Ellis Horwood; 
Miyamoto, T., Takahashi, S., Ito, H., Inagaki, H., Noishiki, Y., Tissue biocompatibility of cellulose and its derivatives (1989) J Biomed Mater Res, 23, pp. 125-133; 
Franz, G., Polysaccharides in pharmacy (1986) Adv Polym Sci, 76, pp. 1-30; 
Philipp, B., Bock, W., Schierbaum, F., Application of polysaccharides and their derivatives as supporting materials and auxiliary substances in medicine and nutrition (1979) J Polym Sci Polym Symp, 66, pp. 83-100; 
Galgut, P.N., Oxidized cellulose mesh. I. Biodegradable membrane in periodontal surgery (1990) Biomaterials, 11, pp. 561-564; 
Skoog, T., The use of periosteum and surgicel{\textregistered} for bone restoration in congenital clefts of the maxilla (1967) Scand J Plast Reconstr Surg, 1, pp. 113-130; 
Degenshein, G.A., Hurwitz, A., Ribacoff, S., Experience with regenerated oxidized cellulose (1963) NY State J Med, 63, pp. 2639-2643; 
Poustis, J., Baquey, C., Chauveaux, D., Mechanical properties of cellulose in orthopaedic devices and related environments (1994) Clin Mater, 16, pp. 119-124; 
Baquey, C., Barbi{\'e}, C., More, N., Rouais, F., Poustis, J., Chauveaux, D., In vivo study of the biostability of a cellulose material (1992) Fourth World Biomaterials Congress, p. 365. , Berlin, Germany, April; 
Brie, J., Lafage, M.H., Chauveaux, D., Gueroult, J.M., Poustis, J., Baquey, C., La cellulose macroporeuse: Un mat{\'e}riau potentiellement utilisable pour le comblement osseux. Biomat{\'e}riaux et organes artificiels (1992) Proceedings of the BIOMAT 92 Congress, , Bordeaux, France, November; 
Pommier, J.C., Poustis, J., Baquey, C., Chauveaux, D., Biocompatible, hydrophilic material. Method of manufacture and uses of same, , Fr Pat No. 8610331, 1986; Eur Pat No. 0256906 A1, 1987; US Pat No. 4904258, 1990; 
Barbi{\'e}, C., Chauveaux, D., Baquey, C., Poustis, J., Pommier, J.C., Morlier, R., Ducassou, D., {\'E}valuation des propriet{\'e}s mecaniques de mat{\'e}riaux cellulosiques et perspectives d{\textquoteright}application originales de ces mat{\'e}riaux en chirurgie orthop{\'e}dique (1987) Calcif Tiss Biomaterials, Proceedings of the BIOMAT 87 Congress, pp. 319-325. , Bordeaux, France, December; 
Li, S., Liu, Q., De Wijn, J., Wolke, J., Zhou, B., De Groot, K., In-vitro apatite formation on phosphorylated bamboo (1997) J Mater Sci: Mater Med, 8, pp. 543-549; 
Mucalo, M.R., Yokogawa, Y., Toryiama, M., Suzuki, T., Kawamoto, Y., Nagata, F., Nishizawa, K., Growth of calcium phosphate on surface-modified cotton (1995) J Mater Sci: Mater Med, 6, pp. 597-605; 
Mucalo, M.R., Yokogawa, Y., Suzuki, T., Kawamoto, Y., Nagata, F., Nishizawa, K., Further studies of calcium phosphate growth on phosphorylated cotton fibres (1995) J Mater Sci: Mater Med, 6, pp. 658-669; 
Granja, P.L., Baquey, C., Pouys{\'e}gu, L., Bareille, R., De J{\'e}so, B., Barbosa, M.A., (1996) Cellulose-based biomaterials for bone regeneration: Hemi-synthesis and characterisation. Polymers in Medicine and Surgery - PIMS{\textquoteright}96, pp. 73-80. , Glasgow, UK; 
Granja, P.L., Barbosa, M.A., Pouys{\'e}gu, L., De J{\'e}so, B., Baquey, C., Cellulose phosphates as biomaterials (1999) Frontiers in biomedical polymers applications 2, pp. 195-225. , Lancaster, PA, USA: Technomic Press; 
Granja, P.L., Pouys{\'e}gu, L., De J{\'e}so, B., Rouais, F., Baquey, C., Barbosa, M.A., Cellulose phosphates as biomaterials. Mineralization of chemically modified regenerated cellulose hydrogels (2001) J Mater Sci: Mater Med, , submitted for publication; 
Granja, P.L., De J{\'e}so, B., Bareille, R., Rouais, F., Baquey, C., Barbosa, M.A., (2001) Cellulose phosphates as biomaterials. In vitro biocompatibility studies, , submitted for publication; 
(1994) {\'E}valuation biologique des dispositifs m{\'e}dicaux. Partie 6: Essais concernant les effets locaux apr{\`e}s implantation; 
Wolf, E., Roser, K., Hahn, M., Wezkerling, H., Delling, G.G., Enzyme and immunohistochemistry on undecalcified bone and bone marrow biopsy after embedding in plastic: A new embedding method for routine application (1992) Virchow Archiv Pathol Anat Histopathol, 420, pp. 17-24; 
Van der Lubbe, H.B.M., Patchin, C., De Groot, K., A simple method for preparing thin (10 μm) histological sections of undecalcified plastic embedded bone with implant (1988) Saint Technol, 63, pp. 171-176; 
Daculsi, G., LeGeros, R.Z., Nery, E., Lynch, K., Transformation of biphasic calcium phosphate ceramics in vivo (1989) J Biomed Mater Res, 23, pp. 883-894; 
Charpak, G., Dominik, W., Zaganidis, N., Optical imaging of the spatial distribution of b-particles emerging from surfaces (1989) Proc Natl Acad Sci USA, 86, pp. 1741-1745; 
Charpak, G., Dominik, W., Fabre, J.P., Gaudaen, J., Sauli, F., Suzuki, M., Studies of light emission by continuously sensitive avalanche chambers (1988) Nucl Instrum Methods A, 269, pp. 142-148; 
Charpak, G., Dominik, W., Fabre, J.P., Santiard, J.C., Sauli, F., Solomey, N., Gaseous detectors with parallel electrodes and anode mesh planes (1979) Nucl Instrum Meth, 161, pp. 19-34; 
Daculsi, G., Passutti, N., Effect of the macroporosity for the osseous substitution of calcium phosphate ceramics (1990) Biomaterials, 11, pp. 86-87; 
Le Huec, J.C., Cl{\'e}ment, D., Brouillaud, B., Barthe, N., Dupuy, B., Foliguet, B., Basse-Cathalinat, B., Evolution of the local calcium content around irradiated β-tricalcium phosphate ceramic implants: In vivo study in the rabbit (1998) Biomaterials, 19, pp. 733-738; 
Bloebaum, D., Bachus, K., Momberger, N., Hofmann, A., Mineral apposition rates of human cancellous bone at the interface of porous coated implants (1994) J Biomed Mater Res, 28, pp. 537-544; 
Gross, U.M., Hard tissue histology (1999) Handbook of biomaterials evaluation: scientific, technical and clinical testing of implant materials. 2nd Ed., pp. 689-719. , von Recum AF, editor. MI, USA: Edwards Brothers, Ann Arbor Science; 
Sakkers, R.J.B., Dalmeyer, R.A.J., De Wijn, J.R., Van Blitterswijk, C.A., Use of bone-bonding hydrogel copolymers in bone: An in vitro and in vivo study of expanding PEO-PBT copolymers in goat femora (2000) J Biomed Mater Res, 49, pp. 312-318; 
Elliot, J.C., (1994) Structure and chemistry of the apatites and other calcium orthophosphates, Studies in Inorganic Chemistry Series, 18. , Amsterdam, The Netherlands: Elsevier; 
Aoki, H., (1994) Medical applications of hydroxyapatite, , Tokyo, Japan: Ishiyaku EuroAmerica; 
Goodman, S., Aspenberg, P., Effects of mechanical stimulation on the differentiation of hard tissues (1993) Biomaterials, 14, pp. 563-569; 
Klemm, D., Philipp, B., Heinze, T., Heinze, U., Wagenknecht, W., (1998) Comprehensive cellulose chemistry, vol. 2, functionalization of cellulose, 2, pp. 133-140. , Weinheim, Germany: Wiley-VCH Verlag. Chapter 4; 
Touey, G.P., Kingsport, T., Preparation of cellulose phosphates, , US Pat No. 2759924, 1956; 
Hayashi, T., Biodegradable polymers for biomedical uses (1994) Prog Polym Sci, 19, pp. 663-702; 
Fricain, J.C., Baquey, C., Dupuy, B., Resorption of corals implanted in diffusion chambers (1995) J Mater Sci: Mater Med, 6, pp. 680-684
},
	month = {2002///},
	pages = {971 - 980},
	abstract = {Femoral implantation of regenerated cellulose hydrogels revealed their biocompatibility, but a complete osseointegration could not be observed. Phosphorylation was therefore envisaged as the means to enhance cellulose bioactivity. In vitro studies showed that regenerated cellulose hydrogels promote bone cells attachment and proliferation but do not mineralize in acellular simulated physiological conditions. On the contrary, phosphorylated cellulose has shown an opposite behavior, by inducing the formation of a calcium phosphate layer in simulated physiological conditions, but behaving as a poor substrate for bone cells attachment and proliferation. In order to investigate the in vivo behavior of these materials, and assess the influence of mineralization induction ability vs. bone cells compatibility, unmodified and phosphorylated cellulose hydrogels were implanted in rabbits for a maximum period of 6 months and bone regeneration was investigated. Despite the difficulties arising from the retraction of cellulose hydrogels upon dehydration during the preparation of retrieved implants, histological observations showed no inflammatory response after implantation, with bone intra-spongious regeneration of cells and the integration of the unmodified as well as the phosphorylated cellulose implants. After a maximum implantation period of 6 months, histological observations, histomorphometry and the measurement of the amount of                        45Ca incorporated in the surrounding tissue indicated a slightly better osseointegration of phosphorylated cellulose, although no significant differences between the two materials were found. {\textcopyright} 2001 Elsevier Science Ltd. All rights reserved.
},
	keywords = {animal experiment, Animal implantation, animal tissue, Animalia, Animals, article, Biocompatibility, Biocompatible Materials, biomaterial, Biomaterials, Bone, bone cell, bone mineralization, Bone Regeneration, Calcium, calcium 45, Calcium Compounds, calcium phosphate, Calcium Radioisotopes, cell adhesion, cell proliferation, Cells, Cellulose, Cellulose phosphate, Chemical modification, controlled study, Dehydration, histology, hydrogel, Hydrogels, implant, in vivo study, Inflammation, materials testing, Microscopy, Electron, Scanning, morphometrics, nonhuman, Oryctolagus cuniculus, Osseointegration, Phosphates, Phosphorylation, priority journal, Prostheses and Implants, rabbit, Rabbits, Regenerated cellulose hydrogels},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037082737\&partnerID=40\&md5=faae93af84ab2762579f90bd2d64eefa},
	author = {Fricain, J.C. and Granja, P.L. and Barbosa, M.A. and De J{\'e}so, B. and Barthe, N. and Baquey, C.}
}
@article {2535,
	title = {Densification route and mechanical properties of Si                    3N
                    4-bioglass biocomposites
},
	journal = {BiomaterialsBiomaterials},
	volume = {23},
	year = {2002},
	note = {Cited By (since 1996): 33Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(01)00194-6PubMed ID: 11771704Language of Original Document: EnglishCorrespondence Address: Santos, J.D.; Sec{\c c}{\~a}o de Materiais, Fac. Eng. da Univ. do Porto (FEUP), Universidade do Porto, Rua Roberto Frias, 4200-465 Porto Cedex, Portugal; email: jdsantos@fe.up.ptChemicals/CAS: Biocompatible Materials; Bioglass; Ceramics; Composite Resins; Silicon Compounds; silicon nitride, 12033-89-5Tradenames: BioglassReferences: Kue, R., Sohrabi, A., Nagle, D., Frondonza, C., Hungerford, D., Enhanced proliferation and osteacalcin production by human osteablast-like cells on silicon nitride ceramic discs (1999) Biomaterials, 20, pp. 1195-1201;Howlett, C., McCartney, E., Ching, W., The effects of silicon nitride ceramic on rabbit skeletal cells and tissues (1989) Clin Orthop, 244, pp. 296-301; 
Hench, L.L., Biomaterials: A forecast for the future (1998) Biomaterials, 19, pp. 1419-1423; 
Radin, S., Ducheyne, P., Falaize, S., Hammond, A., In vitro transformation of bioactive glass granules into Ca-P shells (2000) J Biomed Mater Res, 49, pp. 264-272; 
Santos, J.D., Amaral, M., Oliveira, S.M., Lopes, M.A., Silva, R.F., Silicon Nitride-Bioglass{\textregistered}45S5 composite for biomedical applications (2001) Key Eng Mater, 192-195, pp. 589-592; 
Gazzara, C.P., Messier, D.R., Determination of phase content of Si 3N 4 by X-ray diffraction analysis (1997) Am Ceram Soc Bull, 56 (9), pp. 777-780; 
Niihara, K., Morena, R., Hasselman, D.P., Evaluation of K IC of brittle solids by the indentation method with low crack-to-indent ratios (1982) J Mater Sci Lett, 1, pp. 13-16; 
Standard test method for dynamic Young{\textquoteright}s modulus, shear modulus, and Poisson{\textquoteright}s ratio for advanced ceramics by impulse excitation of vibration; 
Kingery, W.D., Densification during sintering in the presence of a liquid phase. I. Theory (1959) J Appl Phys, 30 (3), pp. 301-306; 
Ziegler, G., Heinrich, J., W{\"o}tting, G., Review: Relationships between processing, microstructure and properties of dense and reaction-bonded silicon nitride (1987) J Mater Sci, 22, pp. 3041-3086; 
Filho, O.P., LaTorre, G.P., Hench, L.L., Effect of crystallization on apatite-layer formation of bioactive glass 45S5 (1996) J Biomed Mater Res, 30, pp. 509-514; 
Evans, A.G., Wilshaw, T.R., Quasi-static solid particle damage in brittle solids I. Observations, analysis and implications (1976) Acta Metall, 24, pp. 939-956; 
Yamamuro, T., Reconstruction of the iliac crest with bioactive glass-ceramic prostheses (1990) Handbook of Bioactive Ceramics, 1, pp. 335-342. , Yamamuro T, Hench LL, Wilson J, editors. Boca Raton: CRC Press; 
Hench, L.L., Bioceramics (1998) J Am Ceram Soc, 81, pp. 1705-1728; 
Ravaglioli, A., Krajewski, A., (1992) Bioceramics - materials, properties and applications, , London: Chapman \& Hall; 
Kong, Y.M., Kim, S., Kim, H.E., Reinforcement of hydroxyapatite bioceramic by addition of ZrO 2 coated with Al 2O 3 (1999) J Am Ceram Soc, 82 (11), pp. 963-968; 
Ducheyne, P., Marcolongo, M., Schepers, E., Bioceramic composites (1993) An Introduction to Bioceramics. Advanced series in ceramics, 1, pp. 281-297. , Hench L, Wilson J, editors. Singapore: World Scientific. Chapter 15
},
	month = {2002///},
	pages = {857 - 862},
	abstract = {The processing route and the final microstructural and mechanical characteristics of a novel biomaterial composite are described. This new material is composed of 70 wt\% Si                        3N
                        4 ceramic phase and 30 wt\% bioglass, the later performing as a liquid sintering aid system and simultaneously providing bioactivity characteristics to the composite. The conditions for fabrication of an almost fully dense material (\~{}98\% of relative density) were pursued. Optimised parameters were 1350{\textdegree}C-40min-30 MPa by hot-pressing technique. The very fast densification rate of the process avoided the crystallisation of the bioglass intergranular phase and therefore its intrinsic properties were maintained. Also, the large amount of glassy phase assured the densification by liquid phase assisted grain rearrangement without Si
                        3N
                        4 phase transformation. The final mechanical properties of the Si
                        3N
                        4-bioglass were as follows: fracture toughness, K
                        IC = 4.4 MPa m
                        1/2; Vickers hardness, H
                        V = 10.3 GPa; Young{\textquoteright}s modulus, E = 197 GPa; bending strength, σ
                        R = 383 MPa; Weibull modulus, m = 8.3. These values provide an attractive set of properties among other bioactive materials, namely by upgrading the main drawback of bioceramics and bioglasses for high-load medical applications, which is the lack of satisfactory fracture toughness. {\textcopyright} 2001 Elsevier Science Ltd. All rights reserved.
},
	keywords = {3N, 4-bioglass composites, article, Bending strength, Biocompatible Materials, biomaterial, Biomaterial composites, Biomaterials, Ceramic materials, ceramics, Composite Resins, Crystallization, Densification, Densification behaviour, density, Elastic moduli, Fracture toughness, glass, hardness, heat, Hot pressing, Hot-pressing, Kinetics, Mechanical properties, phase transition, Phase transitions, priority journal, Si, Silicon compounds, silicon derivative, Sintering, strength, Stress, Mechanical, technique, Thermodynamics, Vickers hardness testing, X-Ray Diffraction, young modulus},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036132099\&partnerID=40\&md5=e7c2bb690245a7c1d0c856ec1e6f7fb0},
	author = {Amaral, M. and Lopes, M.A. and Silva, R.F. and Santos, J.D.}
}
@article {2529,
	title = {A novel urethane containing copolymer as a surface modification additive for blood contact materials},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {13},
	year = {2002},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1023/A:1015789726455Language of Original Document: EnglishCorrespondence Address: Ji, J.; Department of Polymer Science, Zhejiang University, HangZhou 310027, China; email: jianji@mail.hz.zj.cnReferences: Elbert, D., Hubbell, J., (1996) Annu. Rev. Mater., p. 365;Munro, M., Quattrone, A., Ellsworth, S., Kulkarni, P., Eberhart, R., (1981) Trans. Am. Soc. Artif. Intern. Organs., p. 499; 
Park, K., Okano, T., Nojiri, C., Kim, S., (1988) J. Biomed. Mater. Res., p. 977; 
Han, D., Jeong, S., Kim, Y., Min, B., Cho, H., (1991) J. Biomed. Mater. Res., p. 561; 
Ji, J., Feng, L.X., Barbosa, M.A., (2001) Biomaterials, 22, p. 3015; 
Ji, J., Feng, L.X., Qiu, Y.X., Yu, X.J., Barbosa, M.A., (2000) J. Colloid Interfac. Sci., 15, p. 224; 
(2000) J. Colloid Interfac. Sci., 15, pp. 255-260; 
Ji, J., Feng, L.X., Qiu, Y., Yu, X., (2000) Polymer, p. 3713; 
Ishihara, K., Hanyuda, H., Nakabayashi, N., (1995) Biomaterials, p. 873; 
Amiji, M., Park, K., (1993) J. Biomater. Sci. Polym. Ed., p. 217; 
Lamba, N., Woodhouse, K., Cooper, S.L., (1998) Polyurethanes in Biomedical Applications, p. 43. , CRC Press, New York; 
Nelson, K.D., Eisenbaumer, R., Pomerantz, M., Eberhart, R.C., (1996) ASAIO Journal, p. 884; 
Wang, D.A., Ji, J., Feng, L.X., (2000) Macromol. Chem. Phys., p. 1574; 
Wang, D.A., Ji, J., Feng, L.X., (2000) Macromolecules, p. 8472; 
Brash, J.L., Davidson, V.J., (1976) Thromb. Res., p. 249; 
Qiu, Y.X., Yu, X.J., Feng, L.X., (1992) Markromol. Chem., p. 1377; 
Okkema, A.Z., Grasel, T.G., Zdrahala, R.J., Solomon, D.D., Cooper, S.L., (1989) J. Biomater. Sci. Polym. Ed., p. 43; 
Takahara, A., Jujo, A.N., Kajiyama, N.T., (1989) J. Biomater. Sci. Polym. Ed., p. 17
},
	month = {2002///},
	pages = {677 - 684},
	abstract = {Surface modification to develop a biomolecules-presenting surface is of interest, both from a scientific and an industrial point of view. In this research, a penta-block-coupling polymer of warfarin-PEO-MDI-PEO-warfarin was specially designed as the surface modifying additive (SMA). The warfarin-modified polyurethane surfaces were then prepared by dip-coating method. Attenuated total reflection fourier transform infra-red (ATR-FTIR) spectra revealed that the urethane segments in the SMA could penetrate into the hard block of segmented polyurethane (SPU) via intermolecular hydrogen bonds. The X-ray photoelectron spectroscopy (XPS) results indicated that the intermolecular hydrogen bonds were strong enough to form stable warfarin-PEO composite surfaces in an aqueous environment. Fibrinogen and albumin adsorption onto unmodified and SMA-modified SPU was investigated by the                        125l-labeled method. The surface for attracting and reversibly binding albumin, which was proved to suppress the platelet adhesion and prolong the clotting time, has been developed by the simple coating of the novel SMA in SPU. {\textcopyright} 2002 Kluwer Academic Publishers.
},
	keywords = {Adhesion, Adsorption, Albumin, article, Attenuation, biomaterial, Block copolymers, Blood, blood clotting time, Chemical modification, Coatings, copolymer, fibrinogen, Fourier transform infrared spectroscopy, human, human cell, hydrogen bond, Hydrogen bonds, infrared spectroscopy, iodine 125, material coating, Polyurethanes, priority journal, Surface modifying additives (SMA), surface property, Surface treatment, synthesis, thrombocyte adhesion, urethan, warfarin, X ray photoelectron spectroscopy},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0036308146\&partnerID=40\&md5=4767c8a335b26ea0390bc42ddbbaf6b1},
	author = {Ji, J. and Barbosa, M.A. and Feng, L. and Shen, J.}
}
@article {2559,
	title = {Self-assembly and surface structure of an amphiphilic graft copolymer, polystyrene-graft-ω-stearyl-poly(ethylene oxide)},
	journal = {Journal of Colloid and Interface ScienceJ. Colloid Interface Sci.},
	volume = {224},
	year = {2000},
	note = {Cited By (since 1996): 12Export Date: 21 November 2012Source: ScopusCODEN: JCISAdoi: 10.1006/jcis.2000.6729Language of Original Document: EnglishCorrespondence Address: Ji, J.; Instituto de Engenharia Biomedica, Laboratorio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: jian@ibmc.up.ptChemicals/CAS: macrogol, 25322-68-3; polystyrene, 9003-53-6; stearic acid, 57-11-4, 646-29-7References: Brash, J.L., (1983) Biocompatible Polymer, Metals and Composites, p. 35. , M. Szycher. Lancaster: Technomic;Gombotz, W.R., Guanghui, W., Horbett, T.A., Hoffman, A.S., (1991) J. Biomed. Mater. Res., 25, p. 1547; 
Munro, M.S., Quattrone, A.J., Ellsworth, S.R., Kulkarni, P., Eberhart, R.C., (1981) Trans. ASAIO, 17, p. 499; 
Grasel, T.G., Pierce, J.A., Cooper, S.L., (1987) J. Biomed. Mater. Sci., 21, p. 815; 
Duncan, A.C., Sefton, M.V., Brash, J.L., (1997) Biomaterials, 18, p. 1585; 
Keogh, J.R., Velander, F.F., Eaton, W., (1992) J. Biomed. Mater. Sci., 26, p. 441; 
Lee, J.H., Jeong, B.J., Lee, H.B., (1997) J. Biomed. Mater. Sci., 34, p. 105; 
Bots, J.G.F., Van Der Does, L., Bentjes, A., (1986) Biomaterials, 7, p. 393; 
Osterberg, E., Holmberg, K., Schuman, T.P., Riggs, J.A., Burns, N.L., Van Alstine, J.M., Harris, J.M., (1995) J. Biomed. Mater. Res., 29, p. 741; 
Hubbell, J.A., (1997) Polym. Prepr., 38, p. 528; 
Harris, J.M., (1997) Polym. Prepr., 38, p. 520; 
Qiu, Y.X., Yu, X.J., Feng, L.X., (1992) Markromol. Chem., 173, p. 1377; 
Neumann, A.W., Good, R.J., (1979) Surface and Colloid Science, Vol. 11, Experimental Methods, p. 31. , R. J. Good, \& R. R. Stromberg. New York: Plenum Press; 
Ji, J., Feng, L., Qiu, Y., Yu, X., (1998) Macromol. Rapid Commun., 19, p. 473; 
Cameron, G.G., (1989) Comprehensive Polymer Science, Polymer Characterization, p. 517. , S. G. Allen. Oxford: Pergamon Press; 
Andrade, J.D., Ma., S.M., (1979) J. Colloid Interface Sci., 12, p. 488; 
Ranter, B.D., (1985) Surface and Interfacial Aspects of Biomedical Polymers Vol.1, p. 374. , J. D. Andrade. New York: Plenum Press; 
Takahara, A., Jo, N.J., Kajiyama, T., (1989) J. Biomater. Sci. Polym. Ed., 1, p. 17; 
Lide, D.R., (1996) Handbook of Chemistry and Physics, , D. R. Lider. Boca Raton: CRC Press; 
Van Oss, C.J., (1994) Interfacial Forces in Aqueous Media, pp. 170-177. , C. J. Van Oss. New York: Marcel Dekker Inc; 
Gombotz, W.R., Guanhui, W., Horebett, T.A., Hoffman, A.S., (1992) Poly(ethylene Glycol) Chemistry: Biotechnical and Biomedical Application, pp. 247-261. , J. M. Harris. New York: Plenum Press; 
Wu, D., Jian, K., (1995) Sci. China (B), 25, p. 1121; 
Wu, D., Jian, K., (1995) Sci. China (B), 26, p. 409; 
Ji, J., Feng, L., Qiu, Y., Yu, X., Polymer, , in press; 
Hester, J.F., Banerjee, P., Mayes, A.M., (1999) Macromolecules, 32, p. 1643
},
	month = {2000///},
	pages = {255 - 260},
	abstract = {Surface properties of the polystyrene-graft-ω-stearyl-poly(ethylene oxide) (PS-g-SPEO) have been characterized by X-ray photoelectron spectroscopy (XPS), differential scanning calorimetry (DSC), contact angle, and spin probe techniques. The XPS results indicate that the surface and bulk composition of the PS-g-SPEO copolymers differ remarkably from each other. The stearyl and EO components enrich at the copolymer/air interfaces due to the self-assembly of the stearyl groups. At the PS-g-SPEO-72.6 surface (the x in PS-g-SPEO-x indicates the bulk density of the SPEO in wt\%), the self- assembly of the hydrophobic stearyl groups is strong enough to form a stable liquid crystalline phase as indicated by DSC. At polymer/water interfaces, PS-g-SPEO-72.6 presents a hydrophilic surface with low PEO mobility, whereas PS-g-SPEO-50.6 and PS-g-SPEO-31.0 present the hydrophobic surface with high PEO mobility. The two different types of the surfaces, with different characters in surface energy, surface mobility of PEO, and surface architecture of SPEO, will be quite valuable as models for detecting the synergistic action of the PEO chains and the stearyl groups (specific ligand for albumin binding) in protein solutions. (C) 2000 Academic Press.},
	keywords = {Amphiphilic copolymer, article, biomaterial, copolymer, crystal, differential scanning calorimetry, electron microscopy, electron spin resonance, hydrophobicity, macrogol, polystyrene, priority journal, protein assembly, protein binding, protein structure, Self-assembly, stearic acid, Surface structure, X ray analysis},
	isbn = {00219797 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034655268\&partnerID=40\&md5=1628a0cfec8d5ced3e11c87d74ceae44},
	author = {Ji, J. and Feng, L. and Qiu, Y. and Yu, X. and Barbosa, M.A.}
}
@article {2574,
	title = {CaO-P                    2O
                    5 glass hydroxyapatite double-layer plasma-sprayed coating: In vitro bioactivity evaluation
},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {45},
	year = {1999},
	note = {Cited By (since 1996): 31Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(19990615)45:4<376::AID-JBM13>3.0.CO;2-SLanguage of Original Document: EnglishCorrespondence Address: Santos, J.D.; Departamento Engenharia Metalurgica, FEUP, Universidade do Porto, R. dos Bragas, 4099 Porto Codex, PortugalChemicals/CAS: Alloys; Calcium, 7440-70-2; CaO-SiO2-P2O5-CaF2 glass; Coated Materials, Biocompatible; Durapatite, 1306-06-5; Phosphates; Powders; Resin Cements; Solutions; titanium alloy (TiAl6V4), 12743-70-3; Titanium, 7440-32-6References: Denissen, H., Mangano, C., Venini, G., (1985) Hydroxylapatite Implants., , Padua: Piccin Nuova Libraria, S.P.A;Van Raemdonck, W., Ducheyne, P., De Meester, P., Calcium phosphate ceramics (1984) Metal and Ceramic Biomaterials, pp. 143-166. , Ducheyne P, Hastings GW, editors, Boca Raton, FL: CRC Press; 
Legeros, R.Z., Incorporation of magnesium in synthetic and biological apatites (1994) Tooth Enamel IV., pp. 32-36. , Fearnead RW, editor. Amsterdam: Elsevier Science; 
Jha, L.J., Best, S.M., Knowles, J.C., Rehman, I., Santos, J.D., Bonfield, W., Preparation and characterization of fluoride-substituted apatites (1997) J Mater Sci Mater Med, 8, pp. 185-191; 
Geesink, R.G.T., Degroot, K., Chemical implant fixation using hydroxyl-apatite coatings (1987) Clin Orthop, 2, pp. 147-170; 
Radin, S., Ducheyne, P., The effect of plasma sprayed induced changes in the characteristics on the in vitro stability of calcium phosphate ceramics (1990) Transactions of the 16th Annual Meeting of the Society for Biomaterials XIII, pp. 128-130; 
Cook, S.D., Thomas, K.A., Hydroxyapatite coated porous titanium for use as an orthopaedic biologic attachment (1989) Clin Orthop, 230, pp. 303-312; 
McPherson, R., Gane, N., Bastow, T.J., Structural characterization of plasma-sprayed hydroxylapatite coatings (1995) J Mater Sci Mater Med, 6, pp. 327-334; 
De Groot, K., Geesink, R., Klein, C.P.A.T., Serekian, P., Plasma sprayed coatings of hydroxylapatite (1987) J Biomed Mater Res, 21, pp. 1375-1381; 
Ravagliolli, A., Krajewszki, A., Laudadio, P., Presutti, L., Cunsolo, E., Martinetti, R., About ceramics for stapedial prostheses and the ossicular chain (1992) Fifth International Symposium on Ceramics in Medicine-Bioceramics 5, pp. 451-458. , Yamamuro T, Kokubo T, Nakamura T, editors. Kyoto: Kobunshi Kankokai; 
Hastings, G.W., Daily, D., Hydroxyapatite coatings (1989) First International Symposium on Ceramics in Medicine-Bioceramics 1, pp. 355-358. , Oonishi H, Aoki H, Sawai K, editors. Tokyo: Ishiyaku EuroAmerica; 
Aoki, H., Medical applications of hydroxyapatite (1991) Science and Medical Applications of Hydroxyaptite, pp. 175-192. , Aoki H, Sawai K, editors. Tokyo: Takyama Press System Centre; 
Rey, C., Calcium phosphate biomaterials and bone mineral: Differences in composition, structures and properties (1990) Biomaterials, 11, pp. 13-15; 
Evans, G., Behiri, J., Currey J, B.W., Microhardness and young{\textquoteright}s modulus in cortical bone exhibiting a wide range of mineral volume fractions, and in a bone analogue (1990) J Mater Sci Mater Med, 1, pp. 38-43; 
Rey, C., Froche, M., Heughebaert, M., Heughebaert, J.C., Lacout, J.L., Vignoles, M., Apatite chemistry in biomaterial preparation, shaping and biological behaviour (1991) Fourth International Symposium on Ceramics in Medicine-Bioceramics 4, pp. 57-60. , Bonfield W, Hastings GW, Tanner KE, editors. London: Butterworth-Heinemann; 
Knowles, J.C., Talal, S., Santos, J.D., Sintering effects in a glass reinforced hydroxyapatite (1996) Biomaterials, 17, pp. 1437-1442; 
Kokubo, T., Hayashi, T., Sakka, S., Kitsugi, T., Yamamuro, T., Takagi, M., Shibuya, T., Surface structure of a load bearable bioactive glass-ceramic A-W (1981) High Tech Ceramics, pp. 175-184. , Vicenzini P, editor. Amsterdam: Elsevier Science Publisher BV; 
Hench, L., Anderson, O.H., LaTorre, G.P., The kinetics of bioactive ceramics. Part III: Surface reactions for bioactive glasses compared with inactive glass (1991) Fourth International Symposium on Ceramics in Medicine-Bioceramics 4, , Bonfield W, Hastings GW, Tanner KE, editors. London: Butterworth-Heinemann; 155-162; 
Daculsi, G., LeGeros, R.Z., Heughebart, M., Barbieux, I., Formation of a carbonate apatite crystals after implantation of calcium phosphate ceramics (1990) Calcif Tissue Int, 46, pp. 20-27; 
Kokubo, T., Ito, S., Huang, Z.T., Hayashi, T., Sakka, S., Kitsugi, T., Yamamuro, T., Ca-P rich layer formed on high strength bioactive glass-ceramic A-W (1990) J Biomed Mater Res, 24, pp. 331-343; 
Ducheyne, P., Radin, S., Ishikawa, K., Kim, C.S., Dissolution and precipitation of calcium phosphate phases on biomaterials correlates with in vivo bioactivity (1991) Fourth International Symposium on Ceramics in Medicine-Bioceramics 4, pp. 135-144. , Bonfield W, Hastings GW, Tanner KE, editors. London: Butterworth-Heinemann; 
Rey, C., Hina, A., Amrah-Bouali, S., Ranz, X., Surface reactions of calcium-phosphate bioceramics, comparison with bone mineral surface chemistry (1995) Fourth Euro Ceramics., 8, pp. 301-312. , Ravaglioli A, editor. Faenza: Gruppo Editoriale Faenza Editrice; 
Van Blitterswijk, C.A., Grote, J.J., Kuypers, W., Blok-Van Hoek, C.J.G., Daems, W.T., Bioreactions at the tissue/hydroxyapatite interface (1985) Biomaterials, 6, pp. 243-251; 
Ducheyne, P., Bioceramics: Material characteristics versus in vivo behavior (1987) J Biomed Mater Res, 21, pp. 219-236; 
Hyakuna, K., Yamamuro, T., Kotoura, Y., Oka, M., Nakamura, T., Kitsogi, T., Kokubo, T., Kushitani, H., Surface reactions of calcium phosphate ceramics to various solutions (1990) J Biomed Mater Res, 24, pp. 471-488; 
Santos, J.D., Reis, R.L., Monteiro, F.J., Knowles, J.C., Hastings, G.W., Liquid phase sintering of hydroxyapatite by phosphate and silicate glass additions: Structure and properties of the composites (1995) J Mater Sci Mater Med, 6, pp. 348-352; 
Tanahashi, M., Yao, T., Kokubo, T., Minoda, M., Miyamoto, T., Nakamura, T., Yamamuro, T., Apatite coated on organic polymers by biomimetic process: Improvement in adhesion to substrate by HC1 treatment (1995) J Mater Sci Mater Med, 6, pp. 319-326; 
Neo, M., Nakamura, T., Yamamuro, T., Ohtsuki, C., Kokubo, T., Bando, Y., A comparative study of ultrastructures of the interfaces between four kinds of surface-active ceramic and bone (1992) J Biomed Mater Res, 26, pp. 1419-1432; 
Li, P., Ohtsuki, C., Kokubo, T., Nakanishi, K., Soga, N., Nakamura, T., Yamamuro, T., Process of formation of bone-like apatite layer on silica gel (1993) J Mater Sci Mater Med, 4, pp. 127-131; 
Santos, J.D., Jha, L.J., Monteiro, F.J., Surface modifications of glass-reinforced hydroxyapatite composites (1995) Biomaterials, 16, pp. 521-526; 
Neo, M., Kotani, S., Fujita, Y., Yamamuro, T., Bando, Y., Ohtsuki, C., Kokubo, T., Differences in ceramic-bone interface between surface-active ceramics and resorbable ceramics: A study by scanning and transmission electron microscope (1992) J Biomed Mater Res, 26, pp. 255-267; 
Li, P., Yang, Q., Zhang, F., Kokubo, T., The effect of residual glassy phase in a bioactive glass-ceramic on the formation of its surface apatite layer in vitro (1992) J Mater Sci Mater Med, 3, pp. 452-456; 
Kotani, S., Fujita, Y., Kitsugi, T., Nakamura, T., Yamamuro, T., Bone bonding mechanism of β-tricalcium phosphate (1991) J Biomed Mater Res, 25, pp. 1303-1315; 
Daculsi, G., LeGeros Rz, H.M., Barbieux, I., Formation of carbonate-apatite crystals after implantation of calcium phosphate ceramics (1990) Calcif Tissue Int, 46, pp. 20-27; 
Silva, P.L., Monteiro, F.J., Santos, J.D., Knowles, J.C., Adhesion and microstructural characterisation of plasma sprayed hydroxyapatite-glass ceramic coatings onto Ti-6Al-4V substrates (1998) Surf Coatings Technol, 102, pp. 191-196; 
Kokubo, T., Kushitani, H., Ohtsuki, C., Sakka, S., Yamamuro, T., Chemical reaction of bioactive glass and glass-ceramics with a simulated body fluid (1992) J Biomed Mater Res, 3, pp. 79-83; 
Blakeslee, D.M., Dal-Favero, M.E., (1997) NIST X-ray Photoelectron Spectroscopy Database, , Gaitherburg, MD: National Institute of Standards and Technology; 
Anderson, O.H., Kangasniemi, I., Calcium phosphate formation at the surface of bioactive glass in vitro (1991) J Biomed Mater Res, 25, pp. 1019-1030; 
Santos, J.D., Jha, L.J., Monteiro, F.J., In vitro calcium phosphate formation on SiO 2-Na 2O-CaO-P 2O 5 glass reinforced hydroxyapatite composite: A study by XPS analysis (1996) J Mater Sci Mater Med, 7, pp. 181-185; 
Verhoeven, J.A.T., Van Doveren, H., An XPS investigation of the interface of methane, ethyne, ethene and ethane with barium surface (1982) Surf Sci, 123, pp. 369-383
},
	month = {1999///},
	pages = {376 - 383},
	publisher = {John Wiley \& Sons Inc},
	abstract = {Double-layer composite coatings composed of a P                        2O
                        5-based glass/Ca
                        10(PO
                        4)
                        6(OH)
                        2 (HA) mixture top layer and a simple HA underlayer, on Ti-6AI-4V substrates, were prepared using a plasma-spraying technique. The in vitro bioactivity of these coatings was assessed by immersion testing in simulated body fluid. Both scanning electron microscopy (SEM) analysis and the ionic solution changes followed by atomic absorption spectroscopy and the molybdenum blue method demonstrated that these composite coatings induce a faster surface Ca-P layer formation than the simple HA coatings used as a control. X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the Ca-P layer formed was apatite. The combination of SEM and XPS analyses showed that the apatite layer was a calcium-deficient hydroxyapatite with a Ca/P ranging from 1.3 to 1.4 with CO
                        3
                        2- groups contained in the structure.
Double-layer composite coatings composed of a P
                        2O
                        5-based glass/Ca
                        10(PO
                        4)
                        6(OH)
                        2 (HA) mixture top layer and a simple HA underlayer, on Ti-6AI-4V substrates, were prepared using a plasma-spraying technique. The in vitro bioactivity of these coatings was assessed by immersion testing in simulated body fluid. Both scanning electron microscopy (SEM) analysis and the ionic solution changes followed by atomic absorption spectroscopy and the molybdenum blue method demonstrated that these composite coatings induce a faster surface Ca-P layer formation than the simple HA coatings used as a control. X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the Ca-P layer formed was apatite. The combination of SEM and XPS analyses showed that the apatite layer was a calcium-deficient hydroxyapatite with a Ca/P ranging from 1.3 to 1.4 with CO
                        3
                        2- groups contained in the structure.
},
	keywords = {alloy, Alloys, Apatite, article, Atomic absorption spectroscopy, Atomic spectroscopy, Bioactivity, Biocompatibility, biomaterial, Biomaterials, Biomedical engineering, Body fluids, Calcium, Calcium Compounds, CaO SiO2 P2O5 CaF2 glass, CaO-SiO2-P2O5-CaF2 glass, Chemical analysis, chemical composition, Coated Materials, Biocompatible, Double layer coatings, Double-layer coatings, Durapatite, film, glass, Glass plasma spraying, human, Humans, hydroxyapatite, immersion, implant, in vitro study, Ionic solution changes, Magnesium, materials testing, Molybdenum blue, Multilayers, Particle Size, phosphate, Phosphate minerals, Phosphates, Phosphorus Compounds, phosphorus pentoxide, Plasma spraying, potassium, powder, Powders, resin cement, Resin Cements, Scanning electron microscopy, Sodium, solution and solubility, Solutions, structure analysis, Substrates, Surface Properties, surface property, thickness, Tissue, Titanium, titanium alloy (TiAl6V4), Titanium alloys, X ray photoelectron spectroscopy},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033563315\&partnerID=40\&md5=8c0de1e25709302641b203efac50337b},
	author = {Ferraz, M.P. and Monteiro, F.J. and Santos, J.D.}
}
@article {2568,
	title = {Histological effects of iron accumulation on mice liver and spleen after administration of a metallic solution},
	journal = {BiomaterialsBiomaterials},
	volume = {20},
	year = {1999},
	note = {Cited By (since 1996): 15Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(99)00124-6PubMed ID: 10555088Language of Original Document: EnglishCorrespondence Address: Pereira, M.C.; Departamento de Engenharia Quimica, Faculdade de Engenharia, Universidade do Porto, Rua dos Bragas, 4050-123 Porto, Portugal; email: mcsp@fe.up.ptChemicals/CAS: Iron, 7439-89-6; SolutionsReferences: Rheinold, J.B., Trace elements - A selective survey (1975) Clin Chem, 21, p. 476;Crichton, R.R., Ward, R.J., Structure and molecular biology of iron binding-proteins and the regulation of free iron pools (1992) Iron and Human Diseases, pp. 23-75. , R.B. Lauffer. Boca Raton: CRC Press Inc; 
Lynch, S.R., Lipschitz, D.A., Bothwell, T.H., Charlton, R.W., Iron and the reticulo-endothelial system (1974) Iron in Biochemistry and Medicine, p. 563. , A. Jacobs, \& M. Worwood. London: Academic Press; 
Black, J., Mineral metabolism (1981) Biological Performance of Materials: Fundamentals of Biocompatibility. Biomedical Engineering and Instrumentation, 8, pp. 158-168. , R. Plonsey, Katz J.L. New York: Marcel Dekker; 
Sutow, E.J., Pollack, S.R., The biocompatibility of certain stainless steels (1981) Biocompatibility of Clinical Implant Materials, pp. 77-98. , D.F. Williams. Boca Raton, FL: CRC Press; 
Winter, G.D., Tissue reactions to metallic wear and corrosion products in human patients (1974) J Biomed Mater Res Symp, 5, pp. 11-26; 
Winter, G.D., Wear and corrosion products in tissues and the reactions they provoke (1976) Biocompatibility of Implant Materials, pp. 28-54. , In: Williams, DF, editor London; 
Ferguson A.B., Jr., Laing, P.G., Hodge, E.S., The ionization of metal implants in living tissues (1960) J Bone Jt Surg a, 42, pp. 77-90; 
Pohler, O.E.M., Degradation of metallic orthopedic implants (1983) Biomaterials in Reconstructive Surgery, pp. 196-197. , In: Rubin LR, editor London: The C.V. Mosby Company; 
Lux, F., Zeisler, R., Investigations of the corrosive deposition of components of metal implants and the behaviour of biological trace elements in metallosis tissue by means of instrumental multi-element activation analysis (1974) J Radioanal Chem, 19, pp. 289-297; 
Traisnel, M., Le Maguer, D., Hildebrand, H.F., Iost, A., Corrosion of surgical implants (1990) Clin Mater, 5, pp. 309-318; 
Gosztonyi, E., (1983) Iron Metabolism, p. 339. , New York: Plenum Press; 
Weinberg, E.D., Cellular acquisition of iron and the iron-withholding defense against microbial and neoplastic invasion (1992) Iron and Human Diseases, pp. 179-207. , R.B. Lauffer. Boca Raton, FL: CRC Press Inc; 
Pereira, M.L., Pereira, M.C., Sousa, J.P., Evaluation of nickel effects on mouse liver: Tissue response and metal ion accumulation (1995) Biomed Lett, 52, pp. 235-244; 
Pereira, M.C., Pereira, M.L., Sousa, J.P., Evaluation of nickel toxicity on liver, spleen and kidney of mice after administration of high-dose metal ion (1998) J Biomed Mater Res, 40, pp. 40-47; 
Pereira, M.C., Sousa, J.P., Nickel quantification in mice organs by adsorptive cathodic stripping voltammetry using microelectrodes (1997) Electroanalysis, 9, pp. 150-154; 
Pereira, M.C., Pereira, M.L., Sousa, J.P., Adsorptive stripping measurements of iron accumulation in mice kidney using microelectrodes and histological features (1998) J Trace Elements Med Biol, 12, pp. 50-55; 
Van Den Berg, C.M.G., Huang, Z.Q., Determination of iron in seawater using cathodic stripping voltammetry proceeded by adsorptive collection with the hanging mercury drop electrode (1984) J Electroanal Chem, 177, pp. 269-280; 
Smith, G.K., Black, J., Estimation of in vivo type 316L stainless steel corrosion rate from blood transport and organ accumulation data (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 223-247. , A.C. Fraker, \& C.D. Griffin. Philadelphia: American Society for Testing and Materials; 
Barbosa, M.A., (1991) Biomaterials Degradation, p. 227. , Barbosa M.A. The Netherlands: Elsevier Science Publisher; 
Steinemann, S.G., Corrosion of surgical implants in vivo and in vitro tests (1980) Evaluation of Biomaterials, pp. 1-34. , In: Winter GD et al., editors UK: John Wiley \& Sons Inc; 
Smith, G.K., Systemic aspects of metallic implant degradation (1983) Biomaterials in Reconstructive Surgery, pp. 229-242. , In: Rubin LR, London: The C.V. Mosby Company; 
Britton, S.R., Ramm, G.A., Olynyk, J., Singh, R., O{\textquoteright}Neill, R., Bacon, B.R., Pathophysiology of iron toxicity (1994) Progress in Iron Research, pp. 239-253. , C. et al. Hershko. New York: Plenum Press
},
	month = {1999///},
	pages = {2193 - 2198},
	publisher = {Elsevier Science Ltd},
	abstract = {Special attention has been focused on the toxicity of some metallic species released from implanted materials, which accumulate in vital organs over long periods of time. A set of experiments with mice was designed to investigate the individual effects caused by iron in the liver and spleen. Histological features of these organs were evaluated and slight morphological changes were observed during the treatment time suggesting a negative correlation with the duration of the iron treatment. In addition, to associate the histological changes in the organs with iron accumulation an electrochemical method, adsorptive stripping voltammetry, was chosen to quantify the iron levels in these mentioned organs. The accuracy of the proposed method was checked by atomic absorption spectrometry. Both organs showed elevated concentrations of iron, nearly twofold, 7 days after iron administration compared to control organs.Special attention has been focused on the toxicity of some metallic species released from implanted materials, which accumulate in vital organs over long periods of time. A set of experiments with mice was designed to investigate the individual effects caused by iron in the liver and spleen. Histological features of these organs were evaluated and slight morphological changes were observed during the treatment time suggesting a negative correlation with the duration of the iron treatment. In addition, to associate the histological changes in the organs with iron accumulation an electrochemical method, adsorptive stripping voltammetry, was chosen to quantify the iron levels in these mentioned organs. The accuracy of the proposed method was checked by atomic absorption spectrometry. Both organs showed elevated concentrations of iron, nearly twofold, 7 days after iron administration compared to control organs.
},
	keywords = {Absorption spectroscopy, accuracy, Adsorption, Adsorptive stripping voltametry, Adsorptive stripping voltammetry, animal experiment, animal model, animal tissue, Animalia, Animals, article, atomic absorption spectrometry, Atomic spectroscopy, bioaccumulation, biomaterial, controlled study, Corrosion, electrochemistry, histology, histopathology, Implants (surgical), Injections, Subcutaneous, Iron, iron metabolism, Kinetics, Liver, liver toxicity, male, metal implantation, Mice, morphology, mouse, nonhuman, Organ Size, potentiometry, priority journal, quantitative assay, Solutions, Spleen, Stainless steel, tissue distribution, toxicity},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032855084\&partnerID=40\&md5=2e7274d8d0372ff55881c2d9de94c54b},
	author = {Pereira, M.C. and Pereira, M.L. and Sousa, J.P.}
}
@article {2575,
	title = {Hydrophobicity, surface tension, and zeta potential measurements of glass-reinforced hydroxyapatite composites},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {45},
	year = {1999},
	note = {Cited By (since 1996): 46Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(19990615)45:4<370::AID-JBM12>3.0.CO;2-0PubMed ID: 10321710Language of Original Document: EnglishCorrespondence Address: Santos, J.D.; INEB, University of Porto, Rua do Campo Alegre, 823, 4150 Porto, PortugalChemicals/CAS: Biocompatible Materials; Calcium Phosphates; Hydroxyapatites; Proteins; VitronectinReferences: Yamamuro, Y., Hench, L.L., Wilson, J., (1990) CRC Handbook of Bioactive Ceramics, , Boca Raton, FL: CRC Press;Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., Microstructural characterization of glass reinforced hydroxyapatite composites (1994) Biomaterials, 15, pp. 5-10; 
Aoki, H., Medical applications of hydroxyapatite (1991) Science and Medical Applications of Hydroxyapatite, pp. 1-214. , Aoki H, Sawai K, editors. Tokyo: Takyama Press System Center; 
Akao, M., Aoki, H., Kato, K., Mechanical properties of sintered hydroxyapatite for prosthetic applications (1981) J Mater Sci, 16, pp. 809-812; 
Davies, J.E., The importance and measurement of surface charge species in cell behaviour at the biomaterial interface (1988) Surface Characterization of Biomaterials, pp. 219-234. , Ratner BD. New York: Elsevier; 
Ratner, B.D., Biomaterial surfaces (1987) J Biomed Mater Res Appl Biomat, 21, pp. 59-90; 
Manson, S.R., Harker, L.A., Ratner, B.D., Hoffman, A.S., Evaluation of artificial surfaces with a non human primate model of arterial thrombosis (1980) J Lab Clin Med, 95, pp. 289-304; 
Grinnell, F., Milamand, M., Srere, P.A., Studies on cell adhesion (1972) Arch biochem biophys, 153, pp. 193-198; 
Chang, S.K., Hum, O.S., Moscarello, Ma., Neumann, A.W., Zing, W., Leutheusser, M.J., Ruegsegger, B., Platelet adhesion to solid surfaces: The effect of plasma proteins and substrate wettability (1997) Med Progr Technol, 5, pp. 57-66; 
Agathopoulos, S., Nikolopoulos, P., Determination of the energy of the interactions oxides and biological liquids (1995) Fourth Euro Ceramics, 8, pp. 319-324. , Ravaglioli A, editor; 
Lapin, M., Warocquier-Cl{\'e}rout, R., Legris, C., Degrange, M., Luizard, M.F.S., Correlation between roughness and wettability, cell adhesion and cell migration (1997) J Biomed Mater Res, 36, pp. 99-108; 
Williams, D.F., Bagnall, R.D., Adsorption of proteins on polymers and its role in the response of soft tissue (1998) Fundamental Aspects of Biocompatibility, 2, pp. 113-127. , Williams DF, editor. Boca Raton, FL: CRC Press; 
Dimitrov, S., Krachevsky, P.A., Nikolov, A.D., Noshi, H., Matsumoto, M., Contact angle measurements with sessile drops and bubbles (1991) J Colloid Interface Sci, 145, pp. 279-282; 
Adamson, W., (1990) Physical chemistry of surfaces. 5th edition, pp. 4-52. , New York: Wiley; 
Young, T., (1835) Miscellaneous Works, 1, p. 418. , Peacock G, editor London: Murray; 
Young, T., (1805) Philos Trans R Soc Lond, 95, p. 65; 
Correia, N.T., Ramos, J.M., Saramago, B., Calado, J., Estimation of the surface tension of a solid: Application to a liquid crystalline (1997) J Colloid Interface Sci, 189, pp. 361-369; 
Owens, D.K., Wendt, R.D., Estimation of the surface free energy of polymers (1969) J Appl Polym Sci, 13, p. 1741; 
Schrader, M.E., Young dupr{\'e} revisited (1995) Langmuir, 11, p. 3585; 
Kowalchuk, R.M., Pollack, S.R., Ducheyne, P., King, L.A., Particle microelectrophoresis of calcium-deficient hydroxyapatite: Solution composition and kinetics effects (1993) J Biomed Mater Res, 27, pp. 783-790; 
Ducheyne, P., Kim, C.S., Pollack, S.R., The effect of phase differences on the time dependent variation of the zeta potential of hydroxyapatite (1992) J Biomed Mater Res, 26, pp. 147-168; 
Suzuki, T., Nishizawa, K., Yokogawa, Y., Kameyama, T., Time-dependent variation of the surface structure of bioceramics in tissue culture medium and the effect on adhesiveness of cells (1996) J Ferment Bioeng, 81, pp. 226-232; 
Susuki, T., Yamamoto, T., Yoriyama, M., Kameyama, T., Surface instability of calcium phosphates ceramics in tissue culture medium and the effect on adhesion and growth of anchorage-dependent animal cells (1997) J Biomed Mater Res, 34, pp. 507-517; 
Hay, H., (1991) Cell Biology of Extracellular Matrix. 2nd Edition, pp. 127-129. , New York: Plenum Press; 
Seiffert, D., Detection of vitronectin in mineralized bone matrix (1986) J Histochem Cytochem, 44, pp. 275-280; 
Rodan, G.A., Introduction to bone biology (1992) Bone, 12, p. 53; 
Young, M.E., Kerr, J.M., Ibaraki, K., Heegaard, A.M., Gehron, R.P., Structure, expression and regulation of the major matrix proteins of bone (1992) Clin Orthop Rel Res, 281, p. 275; 
Formigli, L., Zecchi, S., Benvenuti, S., Brandi, M.L., Cell to cell interaction on bone tissue (1992) Ann NY Acad Sci, 673, p. 120; 
Underwound, P.A., Bennett, F.A., A comparison of the biological activities of the cell-adhesive proteins vitronectin and fibronectin (1989) J Cell Sci, 93, pp. 641-649; 
Hayman, E.G., Pierschbacher, M.D., Suzuki, S., Ruoslahti, E., Vitronectin: A major cell attachment-promoting protein in foetal bovine serum (1985) Exp Cell Res, 160, pp. 245-250; 
Lopes, Ma., Knowles, J.C., Santos, J.D., Monteiro, F.J., Olsen, I., Flow cytometry for assessing biocompatibility (1998) J Biomed Mater Res, 41, pp. 649-656; 
Lopes, Ma., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass reinforced hydroxyapatite: A comprehensive study of the effect of glass composition on the crystallography of the composite (1998) J Biomed Mater Res, 39, pp. 244-251; 
Cheng, P., Li, D., Boruvka, L., Rotenberg, Y., Neumann, A.W., Automation of axisymmetric drop shape analysis for measurements of interfacial tensions and contact angles (1990) Colloids Surf, 43, pp. 151-167; 
Cheng, P., Neumann, A.W., Computational evaluation of axisymmetric drop shape analysis-profile (ADSA-P) (1992) Colloids Surf, 62, pp. 297-306; 
Saramago, B., Teixeira, M.J., Rosa, M.E., Bordadao, J.C., Influence of the wetting properties of polymeric adhesives on the mechanical behaviour of cork agglomerates (1996) A Adhes Sci Technol, 10, pp. 1111-1127; 
Voigt, A., Thiel, O., Williams, D., Policova, Z., Zingg, W., Neumann, A.W., Asymmetric drop shape analysis (ADSA) applied to protein solution (1991) Colloid Surf, 58, p. 315; 
Williams, R.L., Hunt, J.A., Tengvall, P., Fibroblast adhesion onto methyl-silica gradients with and without preadsorbed proteins (1995) J Biomed Mater Res, 29, pp. 1545-1555; 
Hench, L.L., Ethridge, E.C., (1982) Biomaterials: An Interfacial Approach, , New York: Academic Press; 
Horbert, T.A., Brash, J.L., Proteins at interfaces. II. Fundamentals and applications (1994) ACS Symposium, 602, pp. 1-23. , Chapter 1, Chapter 7; 
Vogler, E.A., Interfacial chemistry in biomaterials science (1993) Wettability, 49, pp. 183-250. , Berg JC, editor New York: Marcel Dekker; Chapter 4
},
	month = {1999///},
	pages = {370 - 375},
	publisher = {John Wiley \& Sons Inc},
	abstract = {Wettability and zeta potential studies were performed to characterize the hydrophobicity, surface tension, and surface charge of P                        2O
                        5-glass- reinforced hydroxyapatite composites. Quantitative phase analysis was performed by the Rietveld method using GSAS software applied to X-ray diffractograms. Surface charge was assessed by zeta potential measurements. Protein adsorption studies were performed using vitronectin. Contact angles and surface tensions variation with time were determined by the sessile and pendent drop techniques, respectively, using ADSA-P software. The highest (- 18.1 mV) and lowest (-28.7 mV) values of zeta potential were found for hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP), respectively, with composite materials presenting values in between. All studied bioceramic materials showed similar solid surface tension. For HA and β-TCP, solid surface tensions of 46.7 and 45.3 mJ/m
                        2, respectively, were obtained, while composites presented intermediate surface tension values. The dispersive component of surface tension was the predominant one for all materials studied. Adhesion work values between the vitronectin solution and HA and β- TCP were found to be 79.8 and 88.0 mJ/m
                        2, respectively, while the 4.0 wt \% glass composites showed slightly lower values than the 2.5 wt \% ones. The presence of β-TCP influenced surface charge, hydrophobicity, and protein adsorption of the glass-reinforced HA composites, and therefore indirectly affected cell-biomaterial interactions.
The hydrophobicity, surface tension, and surface charge of P
                        2O
                        5-glass-reinforced hydroxyapatite (HA) composites were characterized through wettability and zeta potential studies. The highest and lowest values of zeta potential were found for HA and β-TCP. All studied bioceramic materials showed similar solid surface tension. The presence of β-TCP indirectly affected cell-biomaterial interactions.
},
	keywords = {Adhesion, Adsorption, Algorithms, article, Biocompatible Materials, biomaterial, Biomaterials, Bone, Calcium Compounds, calcium phosphate, Calcium phosphates, Cells, Chemical analysis, Chemistry, Physical, Composite materials, Contact angle, glass, Glass reinforced hydroxyapatite composites, hydrophobicity, hydroxyapatite, Hydroxyapatite-glass composites, Hydroxyapatites, implant, Implants (surgical), Interfacial energy, Phosphorus Compounds, Protein adsorption, Proteins, Surface charge, Surface Properties, surface property, Surface tension, Tricalcium phosphate, Vitronectin, wettability, X-Ray Diffraction, Zeta potential},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033563979\&partnerID=40\&md5=3807a20c608ec3dcf8cce1d48213c1a3},
	author = {Lopes, M.A. and Monteiro, F.J. and Santos, J.D. and Serro, A.P. and Saramago, B.}
}
@article {2570,
	title = {Individual study of chromium in the stainless steel implants degradation: An experimental study in mice},
	journal = {BioMetalsBioMetals},
	volume = {12},
	year = {1999},
	note = {Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusCODEN: BOMEEPubMed ID: 10581692Language of Original Document: EnglishCorrespondence Address: Do Carmo Pereira, M.; Departamento de Engenharia Quimica, FEUP, Rua dos Bragas, 4050-123 Porto, Portugal; email: mcsp@fe.up.ptChemicals/CAS: Biocompatible Materials; Chromium, 7440-47-3; Stainless Steel, 12597-68-1References: Bartolozzi, A., Black, J., Chromium concentrations in serum, blood clot and urine from patients following total hip arthroplasty (1985) Biomaterials, 6, pp. 2-8;Black, J., Maitin, E.C., Gelman, H., Morris, D.M., Serum concentrations of chromium, cobalt and nickel after total hip replacement: A six month study (1983) Biomaterials, 4, pp. 160-164; 
Brien, W.W., Salvati, E.A., Healey, J.H., Bansal, M., Ghelman, B., Betts, F., Osteogenic sarcoma arising in the area of a total hip replacement (1990) J Bone Joint Surg, 72 A, pp. 1097-1099; 
Brown, S.A., Farnsworth, U., Merritt, K., Crowe, T.D., In vitro and in vivo metal ion release (1988) J Biomed Mater Res, 22, pp. 321-338; 
Brown, S.A., Zhang, K., Merritt, K., Payer, J.H., In vivo transport and excretion of corrosion products from accelerated anodic corrosion of porous coaled 1-75 alloy (1993) J Biomed Mater Res, 27, pp. 1007-1017; 
Burrows, D., (1983) Chromium: Metabolism and Toxicity, , CRC Press, Boca Raton, Florida; 
Dobbs, H.S., Minski, M.J., Metal ion release after total hip replacement (1980) Biomaterials, 1, pp. 193-198; 
Kargacin, B., Squibb, K.S., Cosentino, S., Zhitkovich, A., Costa, M., Comparison of the uptake and distribution of chromate in rats and mice (1993) Biol Trace Element Res, 36, pp. 307-318; 
Katz, S.A., Salem, H., The toxicology of chromium with respect to its chemical speciation: A review (1993) J Appl Toxicol, 13, pp. 217-224; 
Katz, S.A., Salem, H., (1994) The Biological and Environmental Chemistry of Chromium, , VCH Publishers; 
Langard, S., Hensten-Pettersen, A., Chromium toxicology (1981) Systemic Aspects of Biocompatibility, p. 143. , Williams DF. eds. Boca Raton, Florida, CRC Press; 
Martin, A., Bauer, T.W., Manley, M.T., Marks, K.E., Osteosarcoma at the site of total hip replacement (1988) J Bone Joint Surg, 70 A, pp. 1561-1566; 
Merritt, K., Brown, S.A., Biological effects of corrosion products from metals (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 195-207. , Fraker AC. Griffin CD. eds. Philadelphia, American Society for Testing and Materials; 
Merritt, K., Brown, S.A., Release of hexavalent chromium from corrosion of stainless steel and cobalt-chromium alloys (1995) J Biomed Mater Res, 29, pp. 627-633; 
Merritt, K., Brown, S.A., Sharkey, N.A., Blood distribution of nickel, cobalt, and chromium following intramuscular injection into hamsters (1984) J Biomed Mater Res, 18, pp. 991-1004; 
Merritt, K., Brown, S.A., Sharkey, N.A., The binding of metal salts and corrosion products to cells and proteins in vitro (1984) J Biomed Mater Res, 18, pp. 1005-1015; 
Merritt, K., Crowe, T.D., Brown, S.A., Elimination of nickel, cobalt, and chromium following repeated injections of high dose metal salts (1989) J Biomed Mater Res, 23, pp. 845-862; 
Merritt, K., Fedele, C.D., Brown, S.A., Chromium 6+ or 3+ release during corrosion; And in vivo distribution (1992) Biomaterial- Tissue Interfaces, Advances in Biomaterials, 10, pp. 49-53. , Doherty PJ. et al., eds; 
Pereira, M.C., Faria, J.L., Reis, M.L., Sousa, J.P., Nickel quantification in mice organs by adsorptive cathodic stripping voltammetry using microelectrodes (1997) Electroanalysis, 9, pp. 150-154; 
Pereira, M.C., Pereira, M.L., Sousa, J.P., Adsorptive stripping voltammetric measurements of chromium accumulation in mice organs using mercury film microelectrodes (1997) Electroanalysis, 9, pp. 941-944; 
Pereira, M.C., Pereira, M.L., Sousa, J.P., Evaluation of nickel toxicity on liver, spleen and kidney of mice after administration of high-dose metal ion (1998) J Biomed Mater Res, 40, pp. 40-47; 
Pereira, M.C., Pereira, M.L., Sousa, J.P., Adsorptive stripping measurements of iron accumulation in mice kidney using microlectrodes and histological features (1998) J Trace Elements Med Biol, 12, pp. 50-55; 
Pohler, O.E.M., Degradation of metallic orthopedic implants (1983) Biomaterials in Reconstructive Surgery, pp. 158-228. , Rubin LR, ed. The C. V. Mosby Company; 
Ross, M.H., Rowrell, L.J., (1985) Histology a Text and Atlas, , Williams \& Wilkins; 
Smith, G.K., Black, J., Estimation of in vivo type 316L stainless steel corrosion rate from blood transport and organ accumulation data (1985) Corrosion and Degradation of Implant Materials; Second Symposium, pp. 223-247. , Fraker AC, Griffin CD, eds. American Philadelphia, Society for Testing and Materials; 
Sunderman F.W., Jr., Hopfer, S.M., Swift, T., Cohalt, chromium, and nickel concentrations in body fluids of patients with porous-coated knee or hip prostheses (1989) J Orthop Res, 7, pp. 307-315; 
Yang, J., Black, J., Competitive binding of chromium, cobalt and nickel to serum proteins (1994) Biomaterials, 15, pp. 262-268; 
Young, E., Houwing, R.H., Patch test results with standard allergens over a decade (1987) Contact Dermatitis, 17, pp. 104-107
},
	month = {1999///},
	pages = {275 - 280},
	abstract = {To study the accumulation and the histological effects in mice organs caused by hexavalent chromium, one of the corrosion products released from AISI 316L stainless steel implants, mice groups were subcutaneously injected with a metallic solution of chromium during a certain period of time. Similar injections were made with HBSS (Hank{\textquoteright}s Balanced Salt Solution) in other groups to be used as controls. The levels of chromium found in the liver, kidney and spleen of the control and the treated animals were obtained by atomic absorption spectrometry (AAS) and were compared to those obtained by AdSV (adsorptive stripping voltammetry) to test the accuracy of the results. During the experimental period, the liver and spleen showed a progressive and significant accumulation of chromium whereas in the kidney the significant accumulation found after the first week practically remained unchanged during the four weeks. Apparently, the histological analysis of these tissues did not evidence any relevant morphological alteration induced by the chromium accumulations during the four weeks of treatment.},
	keywords = {animal experiment, animal tissue, Animalia, Animals, article, atomic absorption spectrometry, Biocompatible Materials, biomaterial, body weight, Chromium, controlled study, Corrosion, histology, histopathology, implant, Injections, Subcutaneous, Kidney, Liver, liver level, male, Mice, Mice organs, Mice, Inbred Strains, mouse, nonhuman, potentiometry, Prostheses and Implants, Spleen, Stainless steel, tissue distribution},
	isbn = {09660844 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032840626\&partnerID=40\&md5=e81da6070ec455e49604d3773033d58d},
	author = {Pereira, M.D.C. and Pereira, M.D.L. and Sousa, J.P.}
}
@article {2588,
	title = {Adsorptive stripping measurements of iron accumulation in mice kidney using microelectrodes and histological features},
	journal = {Journal of Trace Elements in Medicine and BiologyJ. Trace Elem. Med. Biol.},
	volume = {12},
	year = {1998},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: JTEBFPubMed ID: 9638613Language of Original Document: EnglishCorrespondence Address: Sousa, J.P.; INEB-Inst. de Engenharia Biomedica, Departamento de Engenharia Quimica, Faculdade Engenharia Univ. do Porto, Praca do Coronel Pacheco, 1, 4050 Porto, PortugalChemicals/CAS: Iron, 7439-89-6; Mercury, 7439-97-6References: Traisnel, M., Le Maguer, D., Hildebrand, H.F., Iost, A., Corrosion of surgical implants (1990) Clin. Mater., 5, pp. 309-318;Sutow, E.J., Pollack, S.R., The biocompatibility of certain stainless steels (1981) Biocompatibility of Clinical Implant Materials, 1, pp. 77-98. , Williams, D.F. eds CRC Press, Boca Raton, Florida; 
Ferguson Jr., A.B., Laing, P.G., Hodge, E.S., The ionization of metal implants in living tissues (1960) J. Bone Joint Surg., 42 A, pp. 77-90; 
Winter, G.D., Wear and corrosion products in tissues and the reactions they provoke (1976) Biocompatibility of Implant Materials, pp. 28-54. , Williams, D.F. eds London; 
Lux, F., Zeisler, R., Investigations of the corrosive deposition of components of metal implants and the behaviour of biological trace elements in metallosis tissue by means of instrumental multi-element activation analysis (1974) J. Radioanal. Chem., 19, pp. 289-297; 
Merritt, K., Brown, S.A., Sharkey, N.A., Blood distribution of nickel, cobalt, and chromium following intramuscular injection into hamsters (1984) J. Biomed. Mater. Res., 18, pp. 991-1004; 
Merritt, K., Brown, S.A., Biological effects of corrosion products from metals (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 195-207. , Fraker, A.C., Griffin, C.D. eds American Society for Testing and Materials, Philadelphia; 
K. Merritt, K.A., Brown, S.A., Metal sensitivity reactions to bone screws in rabbits (1982) Clinical Applications of Biomaterials, pp. 85-93. , John Wiley \& Sons Ltd; 
Woodman, J.L., Black, J., Jimenez, S., Organometallic corrosion products of 316L SS HS-21 in serum: In vitro and in vivo comparison in an acute rat model (1982) Biomaterials 1980, pp. 245-250. , Winter, G.D., Gibbons, D.F., Plenk, Jr., H. John Wiley \& Sons Ltd; 
Merritt, K., Crowe, T.D., Brown, S.A., Elimination of nickel, cobalt, and chromium following repeated injections of high dose metal salts (1989) J. Biomed. Mater. Res., 23, pp. 845-862; 
Pereira, M.L., Pereira, M.C., Sousa, J.P., Evaluation of nickel effects on mouse liver: Tissue response and metal ion accumulation (1995) Biomedical Letters, 52, pp. 235-244; 
Pereira, M.C., Pereira, M.L., Sousa, J.P., Evaluation of nickel toxicity on liver, spleen and kidney of mice after administration of high-dose metal ion (1997) J. Biomed. Mater Res., pp. XXXXXX. , in press; 
Schafer, B.A.I., Cheron, R.G., Dluhy, R., Cooper, B., Gleason, R.E., Soeldner, J.S., Bunn, H.F., Clinical consequences of acquired transfusional iron overload in adults (1981) N Engl. J. Med., 304, p. 319; 
Black, J., Mineral metabolism (1981) Biological Performance of Materials: Fundamentals of Biocompatibility. Biomedical Engineerins and Instrumentation Series, pp. 158-168. , Plonsey R., Katz, J.L. Marcel Dekker; 
Bernat, I., Iron deficiency (1983) Iron Metabolism, pp. 215-274. , Plenum Press, New York; 
Nakamura, K., Watanabe, H., Orii, H., Direct measurement of iron in serum by electrothermal atomic absorption spectrometry (1980) Anal. Chim. Acta., 108, pp. 67-71; 
Van Beek, H., Greefkes, H.C.A., Baars, A.J., Determination of copper, iron, manganese, lead and cadmium in automatically wet-digested animal tissue by graphite-furnace atomic absorption spectrometry with Zeeman background correction (1993) Talanta., 34, pp. 580-582; 
Van Wyck, D.B., Schifman, R.B., Stivelman, J.C., Ruiz, J., Martin, D., Rapide sample preparation for determination of iron in tissue by closed-vessel digestion and microwave energy (1988) Clin. Chem., 34 (6), pp. 1128-1130; 
Chakraborti, D., Burguera, M., Burguera, J.L., Analysis of standard reference materials after microwave-oven digestion in open vessels using graphite furnace atomic absorption spectrophotometry and Zeeman-effect background correction (1993) Fresenius J. Anal. Chem., 347, pp. 233-237; 
Lugowski, S., Smith, D.C., Van Loon, J.C., The determination of At, Cr, Co, Fe, and Ni in whole blood by electrothermal atomic absorption spectrophotometry (1987) J. Biomed. Mater. Res., 21, pp. 657-674; 
Wightman, R.M., Microvoltammetric electrodes (1981) Anal. Chem., 53, pp. 1125A-1134A; 
Pletcher, D., Why microelectrodes? (1991) Microelectrodes: Theory and Applications, pp. 3-16. , Montenegro, M.I., Queir{\'o}s, M.A., Daschbach, J.L eds NATO-ASI, Kluwer Academic Publishers, the Netherlands; 
Wojciechowski, M., Go, W., Osteryoung, J., Square-wave anodic stripping analysis in the presence of dissolved oxygen (1984) Anal. Chem., 57, pp. 155-158; 
Kalvoda, R., Kopanica, M., (1989) Pure \& Appl. Chem., 61, pp. 97-112; 
Wang, J., Principles (1985) Stripping Analysis, pp. 9-64. , VCH publishers, Inc; 
Perejra, M.C., Faria, J.L., Reis, M.L., Sousa, J.P., Nickel quantification in mice organs by adsorptive cathodic stripping voltammetry using mercury microelectrodes (1996) Electroanalysis, 9, pp. 150-154; 
Van Den Berg, C.M.G., Huang, Z.Q., Determination of iron in seawater using cathodic stripping voltammetry preceded by adsorptive collection with the hanging mercury drop electrode (1984) J. Electroanal. Chem., 177, pp. 269-280; 
Morais, S., Carvalho, G.S., Sousa, J.P., Iron determination in osteoblast-like cell cultures medium by adsorptive cathodic stripping voltammetry with mercury microelectrodes (1997) Electroanalysis, 9; 
Wang, J., Mannino, S., Application of adsorptive stripping voltammetry to the speciation and determination of iron (III) and total iron in wines (1989) Analyst, 114, pp. 643-645; 
Smith, G.K., Black, J., Estimation of in vivo type 316L stainless steel corrosion rate from blood transport and organ accumulation data (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 223-247. , Fraker, A.C. and Griffin. Eds. American Society for Testing Materials; 
Pereira, M.C., Sousa, J.P., Determination of total iron in biological sample solutions with mercury microelectrodes (1996) Portugaliae Electrochimica Acta, 14, pp. 273-278; 
Pereira, M.C., Pereira, M.L., Sousa, J.P., Adsorptive stripping voltammetric measurements of chromium accumulation in mice organs using mercury microelectrodes (1997) Electroanalysis, 9, pp. 941-944
},
	month = {1998///},
	pages = {50 - 55},
	abstract = {The toxic effects caused by iron in kidney was performed on experimental studies with mice following administration of a metallic solution of this specie to simulate the iron corrosion products of a metallic implant. To quantify the total levels of iron present in this organ, an electrochemical method was chosen based on the application of square wave voltammetry using adsorptive collection of iron-catechol on a mercury film microelectrode (MFM). The optimal working conditions to produce a very stable and reproducible iron peak in the digested kidney samples were found to be pH=7.2 provided by 8.0 mmol/L PIPES buffer, catechol concentration of 3.0x10},
	keywords = {, biomaterials, Adsorptive stripping, animal experiment, animal model, animal tissue, Animals, article, atomic absorption spectrometry, biomaterial, controlled study, electrochemical analysis, electrochemistry, histology, Iron, iron absorption, Kidney, male, Mercury, metal implantation, Mice, microelectrode, Microelectrodes, mouse, nonhuman, potentiometry, priority journal, Toxicology},
	isbn = {0946672X (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031746863\&partnerID=40\&md5=f85b47bb916eab32df5be2219498793f},
	author = {Pereira, M.C. and Pereira, M.L. and Sousa, J.P.}
}
@article {2587,
	title = {Evaluation of nickel toxicity on liver, spleen, and kidney of mice after administration of high-dose metal ion},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {40},
	year = {1998},
	note = {Cited By (since 1996): 27Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(199804)40:1<40::AID-JBM5>3.0.CO;2-RPubMed ID: 9511097Language of Original Document: EnglishCorrespondence Address: Sousa, J.P.; Instituto de Engenharia Biomedica, Faculdade de Engenharia, Universidade do Porto, Praca Coronel Pacheco, 1, 4050 Porto, PortugalChemicals/CAS: Nickel, 7440-02-0References: Cigada, A., Rondelli, G., Vicentini, B., Brunella, F., Dallaspezia, G., Corrosion behavior of high nitrogen stainless steels for biomedical applications (1994) Compatibility of Biomedical Implants, pp. 185-195. , P. Kovacs and N. S. Istephanous (eds.), The Electrochemical Society, Inc., Pennington, New Jersey;Bumgardner, J.D., Lucas, L.C., An evaluation of the corrosion properties and cellular compatibility of nickel-based dental casting alloys (1994) Compatibility of Biomedical Implants, pp. 241-252. , P. Kovacs and N. S. Istephanous (eds.), The Electrochemical Society, Inc., Pennington, New Jersey; 
Williams, D.F., (1990) Encyclopedia of Medical and Dental Materials, , Pergamon Press, Oxford; 
Muller, A.W.J., Maessen, F.J.M.J., Davidson, C.L., Determination of the corrosion rates of six dental NiCrMo alloys in an artificial saliva by chemical analysis of the medium using ICP-AES (1990) Dent. Mater., 6, pp. 63-68; 
Bumgardner, J.D., Lucas, L.C., Surface analysis of nickel-chromium dental alloys (1993) Dent. Mater., 9, pp. 252-259; 
Williams, D.F., (1981) Systemic Aspects of Biocompatibility, 1-2. , CRC Press, Boca Raton, Florida; 
Tracana, R.B., Pereira, M.L., Abreu, A.M., Sousa, J.P., Carvalho, G.S., Stainless steel products cause alterations on mouse spleen cellular populations (1995) J. Mater. Sci. Mater. Med., 6, pp. 56-61; 
Tracana, R.B., Sousa, J.P., Carvalho, G.S., Mouse inflammatory response to stainless steel corrosion products (1994) J. Mater. Sci. Mater. Med., 5, pp. 596-600; 
Jacks, J.J., Skiper, A.K., Black, J., Urban, R.M., Galante, J.O., Release and excretion of metal in patients who have a total hip replacement component made of titanium base alloy (1991) J. Bone Joint Surg., 73 A, pp. 1475-1486; 
Stulberg, B.N., Merrit, K., Bawer, T.W., Metallic wear debris in metal backed patellar failure (1994) J. Appl. Biomater., 5, pp. 9-16; 
Panigutti, M.A., Merrit, K., Bruner, R.J., Kraay, M.J., Brown, S.A., Correlation of allergy, metal levels, implant alloy, and implant damage in patients undergoing revision joint arthroplastics (1992) Trans. Soc. Biomater., 15, p. 7; 
Abreu, A.M., Tracana, R.B., Carvalho, G.S., Sousa, J.P., Determination of metal ions released from metallic biomaterials using absorption spectroscopy and microelectrodes (1995) Biomed. Lett., 52, pp. 133-148; 
(1986) Health Assessment Document for Nickel and Nickel Compounds, , EPA/600/8-83/012FF, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina; 
Black, J., (1988) Orthopedic Biomaterials in Research and Practice, , Churchill Livingstone, New York; 
Nieboer, E., Rossetto, F.E., Menon, C.R., Toxicology of nickel compounds (1980) Nickel Toxicology, pp. 359-401. , S. S. Brown and F. W. Sunderman, Jr. (eds.), Academic Press, Inc., New York; 
Pereira, M.L., Pereira, M.C., Sousa, J.P., Evaluation of nickel effects on mouse liver: Tissue response and metal ion accumulation (1995) Biomed. Lett., 52, pp. 235-244; 
Pereira, M.C., Faria, J.L., Reis, M.L., Sousa, J.P., Nickel quantification in mice organs by adsorptive stripping voltammetry using mercury microelectrodes (1996) Electroanalysis, 9, pp. 1-5; 
Black, J., Systemic distribution and excretion (1981) Biomedical Engineering and Instrumentation, p. 180. , R. Plonsey and J. L. Katz (eds.), Marcel Dekker, Inc., New York; 
Pohler, O.E.M., Degradation of metallic orthopedic implants (1983) Biomaterials in Reconstructive Surgery, p. 197. , L. R. Rugin (ed.), The C. V. Mosby Company, St. Louis; 
Ross, M.H., Romrell, L.J., (1989) Histology - A Text and Atlas, , Williams and Wilkins, New York
},
	month = {1998///},
	pages = {40 - 47},
	publisher = {John Wiley \& Sons Inc},
	abstract = {The toxic effects caused by nickel (Ni) per si were explored by performing in vivo studies on mice following subcutaneous administration of a metallic solution of nickel at 1, 2, 3, and 4 weeks to evaluate the side effects of this metal ion when released from stainless steel implants. Other groups were similarly injected with HBSS and used as controls. Accumulation of Ni ions on liver, spleen, and kidney was assessed by an electrochemical method, adsorptive stripping voltammetry (AdSV) using microelectrodes, and atomic absorption spectrometry (AAS). Alterations of those organs induced by Ni ions were studied, showing that several histological changes had been induced. Chemical analysis and histological features indicate that Ni is partially accumulated in the study organs.The toxic effects caused by nickel (Ni) per si were explored by performing in vivo studies on mice following subcutaneous administration of a metallic solution of nickel at 1, 2, 3, and 4 weeks to evaluate the side effects of this metal ion when released from stainless steel implants. Other groups were similarly injected with HBSS and used as controls. Accumulation of Ni ions on liver, spleen, and kidney was assessed by an electrochemical method, adsorptive stripping voltammetry (AdSV) using microelectrodes, and atomic absorption spectrometry (AAS). Alterations of those organs induced by Ni ions were studied, showing that several histological changes had been induced. Chemical analysis and histological features indicate that Ni is partially accumulated in the study organs.
},
	keywords = {Absorption spectroscopy, Adsorptive stripping voltammetry, animal experiment, animal tissue, Animals, article, atomic absorption spectrometry, biomaterial, body weight, controlled study, Dose-Response Relationship, Drug, electrochemistry, Evaluation Studies, histology, Implants (surgical), Kidney, Liver, liver toxicity, Living systems studies, Mice, Microelectrodes, mouse, nephrotoxicity, Nickel, nonhuman, potentiometry, Spectrophotometry, Atomic, Spleen, spleen injury, Stainless steel, toxicity},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032053959\&partnerID=40\&md5=b1b91f83e841302830f88702c7146ae0},
	author = {Pereira, M.C. and Pereira, M.L. and Sousa, J.P.}
}
@article {2580,
	title = {Flow cytometry for assessing biocompatibility},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {41},
	year = {1998},
	note = {Cited By (since 1996): 33Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(19980915)41:4<649::AID-JBM17>3.0.CO;2-9PubMed ID: 9697038Language of Original Document: EnglishCorrespondence Address: Olsen, I.; Department of Periodontology, Eastman Dental Institute, University of London, 256 Gray{\textquoteright}s Inn Rd., London WC1X 8LD, United KingdomChemicals/CAS: Biocompatible Materials; Collagen, 9007-34-5; Dental Implants; Fibronectins; Osteocalcin, 104982-03-8References: Kirkpatrick, C.J., A critical view of current and proposed methodologies for biocompatibility testing: Cytotoxicity in vitro (1992) Regul. Affairs, 4, pp. 13-32;Pizzoferrato, A., Ciapetti, G., Stea, S., Cenni, E., Ariola, C.R., Granchi, D., Savarino, L., Cell culture methods for testing biocompatibility (1994) Clin. Mater., 15, pp. 173-190; 
Puleo, D.A., Preston, K.E., Shaffer, J.B., Bizios, R., Examination of osteoblast-orthopaedic biomaterial interactions using molecular techniques (1993) Biomaterials, 14, pp. 111-114; 
Clifford, C.J., Downes, S., A comparative study of the use of colorimetric assays in the assessment of biocompatibility (1996) J. Mater. Sci. Mater. Med., 7, pp. 637-643; 
Macey, M.G., (1994) Flow Cytometry: Clinical Applications, , Blackwell Scientific Publications, Oxford; 
Salzman, G.C., Singham, S.B., Johnston, R.G., Bohren, C.F., Light scattering and cytometry (1990) Flow Cytometry and Sorting, pp. 81-107. , M. R. Melamed, T. Lindmo, and M. L. Mendelsohn (eds.), Wiley-Liss, New York; 
Corver, W.E., Cornelisse, C.J., Fleuren, G.J., Simultaneous measurement of two cellular antigens and DNA using fluorescein-isothiocyanate, R-phycoerythrin and propidium iodide on a standard FACScan (1994) Cytometry, 15, pp. 117-128; 
Macon, W.R., Salhany, K.E., TI- T-cell subset analysis of paraffin-embedded peripheral T-cell lymphomas (PTCLs) with correlation of CD4/CD8 results by flow cytometry (FACs) (1997) Lab. Invest., 76, p. 753; 
Kuru, L., Parkar, M.H., Griffiths, G.S., Newman, H.N., Olsen, I., Flow cytometry analysis of functionally distinct gingival and periodontal ligament cells (1998) J. Dent. Res., 77, pp. 555-564; 
Kamiya, I., Okuda, K., Hara, K., Flow cytometric identification and detection of Porphromonas gingivalis by an LPS specific monoclonal antibody (1994) J. Periodontol., 65, pp. 309-315; 
Sumner, H., Abraham, D., Bou-Gharios, G., Plater-Zyberk, C., Olsen, I., Simultaneous measurement of cell surface and intracellular antigens by multiple flow cytometry (1991) J. Immunol. Methods, 136, pp. 259-267; 
Beauchamp, J.R., Abraham, D.J., Bou-Gharios, G., Partridge, T.A., Olsen, I., Expression and function of adhesion molecules during differentiation of human skeletal muscle in culture (1992) Am. J. Pathol., 140, pp. 387-401; 
Abraham, D., Lupoli, S., McWhirter, A., Plater-Zyberk, C., Piela, T.H., Korn, J., Olsen, I., Black, C., Expression and function of surface antigens on scleroderma fibroblasts (1991) Arthritis Rheum., 34, pp. 1164-1172; 
Bou-Gharios, G., Osman, J., Black, C., Olsen, I., Excess matrix accumulation in scleroderma is caused partly by differential regulation of stromelysin and TIMP-1 synthesis (1994) Clin. Chim. Acta, 231, pp. 69-78; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., Microstructural characterisation of glass-reinforced hydroxyapatite composites (1994) Biomaterials, 15, pp. 5-10; 
Best, S., Sim, B., Kayser, M., Downes, S., The dependence of osteoblastic response on variations in the chemical composition and physical properties of hydroxyapatite (1997) J. Mater. Sci. Mater. Med., 8, pp. 97-103; 
Martin, J.Y., Schwartz, Z., Hummert, T.W., Schraub, D.M., Simpson, J., Lankford, J., Dean, D.D., Boyan, B.D., Effect of titanium surface roughness on proliferation, differentiation and protein synthesis of human osteoblast-like cells (MG-63) (1995) J. Biomed. Mater. Res., 29, pp. 389-401; 
Granchi, D., Stea, S., Ciapetti, G., Savarino, L., Cavedagna, D., Pizzoferatto, A., In vitro effects of bone cements on the cell cycle of osteoblast-like cells (1995) Biomaterials, 16, pp. 1187-1192; 
Clover, J., Gowen, M., Are MG63 and HOS TE 85 human osteosarcoma cell lines representative models of the osteoblastic phenotype? (1994) Bone, 15, pp. 585-591; 
Scharla, S.H., Strong, D.D., Rosen, C., Mohan, S., Holick, M., Baylink, D.J., Linkhart, T.A., 1,25-Dihydroxyvitamin D3 increases secretion of insulin-like growth factor binding protein-4 (IGFBP-4) by human osteoblast-like cells in vitro and elevates IGFBP-4 serum levels in vivo (1993) J. Clin. Endocrinol. Metabol., 77, pp. 1190-1197; 
De Bart, A.C., Quax, P.H., Lowik, C.W., Verheijen, J.H., Regulation of plasminogen activation, matrix metalloproteinases and urokinase-type plasminogen activator-mediated extracellular matrix degradation in human osteosarcoma cell line MG63 by interleukin-1 alpha (1995) J. Bone Min. Res., 19, pp. 1374-1384; 
Barille, S., Collette, M., Bataille, R., Amiot, M., Myeloma cells upregulate interleukin-6 secretion in osteoblastic cells through cell-to-cell contact but downregulate osteocalcin (1995) Blood, 86, pp. 3151-3159; 
Maillard, C., Malaval, L., Delmas, P.D., Immunological screening of SPARC/osteonectin in nonmineralized tissue (1992) Bone, 13, pp. 257-264; 
Bou-Gharios, G., Osman, J., Atherton, A., Monoghan, P., Vancheeswaran, R., Black, C., Olsen, I., Expression of ectopeptidases in scleroderma (1995) Ann. Rheum. Dis., 54, pp. 111-116; 
Goseki, T., Shimizu, N., Iwasawa, T., Takiguchi, H., Abiko, Y., Effects of in vitro cellular aging on alkaline phosphatase, cathepsin activities and collagen secretion of human peridontal ligament-derived cells (1996) Mech. Age. Dev., 91, pp. 1761-11183; 
Hallab, N.J., Bundy, K.J., O{\textquoteright}Connor, K., Clark, R., Moses, R.L., Cell adhesion to biomaterials: Correlations between surface charge, surface roughness, adsorbed protein and cell morphology (1995) J. Long-Term Effects Med. Implants, 5, pp. 209-231; 
Kieswetter, K., Schwartz, Z., Hummert, T.W., Cochran, D.L., Simpson, J., Sean, D.P., Boyan, B.D., Surface roughness modulates the local production of growth factors and cytokines by osteoblast-like MG-63 cells (1996) J. Biomed. Mater. Res., 32, pp. 55-63; 
Altankov, G., Grinnell, F., Groth, T., Studies on the biocompatibility of materials: Fibroblast reorganization of substratumbound fibronectin on surfaces varying in wettability (1996) J, Biomed. Mater. Res., 30, pp. 385-391; 
Chesmel, K.D., Clark, C.C., Brighton, C.T., Black, J., Cellular response to chemical and morphologic aspects of biomaterial surfaces. II. The biosynthetic and migratory response of bone cell populations (1995) J. Biomed. Mater. Res., 29, pp. 1101-1110; 
Nishizawa, K., Toriyama, M., Suzuki, T., Kawamoto, Y., Yokogawa, Y., Nagae, H., Effects of the surface wettability and zeta potential of bioceramics on the adhesiveness of anchorage-dependent animal cells (1993) J. Ferment. Bioeng., 75, pp. 435-437; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass reinforced hydroxyapatite: A comprehensive study of the effect of glass composition on the crystallography of the composite (1998) J. Biomed. Mater. Res., 39, pp. 244-251
},
	month = {1998///},
	pages = {649 - 656},
	publisher = {John Wiley \& Sons Inc},
	abstract = {Flow cytometry (FCM) was examined as a possible procedure for measuring in vitro the biocompatibility of implant materials for orthopedic and dental surgery. The human osteoblast-like cell line MG63 was grown on hydroxyapatite (HA) and P                        2O
                        5 glass-reinforced HA composite discs and compared with the same cells grown on polystyrene culture dishes. While morphological observation at the light and electron microscopic levels showed no major deleterious effects, FCM indicated that cell size was somewhat reduced, particularly by growth on the HA composite. Moreover, this material also appeared to delay the progression of the cells from the G0/G1 into the S phase of the cell cycle. In addition to this low level of inhibition of cell growth relative to control cultures, FCM analysis also demonstrated that the glass-reinforced HA caused some down-regulation of the expression of osteocalcin and fibronectin, two antigens which play a vital part in the integrity and function of bone and soft connective tissue, respectively. These results thus show, first, that although HA and the HA composite used in these experiments were generally biocompatible, they nevertheless had certain suboptimal effects on the cells; and second, that FCM could be a highly useful procedure for effectively screening and evaluating important biological responses to implant materials.
Flow cytometry (FCM) was examined as a possible procedure for measuring in vitro the biocompatibility of implant materials for orthopedic and dental surgery. The human osteoblast-like cell line MG63 was grown on hydroxyapatite (HA) and P
                        2O
                        5 glass-reinforced HA composite discs and compared with the same cells grown on polystyrene culture dishes. While morphological observation at the light and electron microscopic levels showed no major deleterious effects, FCM indicated that cell size was somewhat reduced, particularly by growth on the HA composite. Moreover, this material also appeared to delay the progression of the cells from the G0/G1 into the S phase of the cell cycle. In addition to this low level of inhibition of cell growth relative to control cultures, FCM analysis also demonstrated that the glass-reinforced HA caused some down-regulation of the expression of osteocalcin and fibronectin, two antigens which play a vital part in the integrity and function of bone and soft connective tissue, respectively. These results thus show, first, that although HA and the HA composite used in these experiments were generally biocompatible, they nevertheless had certain suboptimal effects on the cells; and second, that FCM could be a highly useful procedure for effectively screening and evaluating important biological responses to implant materials.
},
	keywords = {antigen expression, Antigens, Biocompatibility, Biocompatible Materials, biomaterial, Biomaterials, Biomedical engineering, Bone and Bones, bone prosthesis, Cell culture, cell cycle, cell cycle s phase, Cell Line, Cell size, cell ultrastructure, Cells, Collagen, conference paper, Dental Implants, dentistry, extracellular matrix, Fibronectin, Fibronectins, flow cytometry, Glass fiber reinforced plastics, growth inhibition, human, human cell, Humans, hydroxyapatite, Implants, Implants (surgical), Microscopy, Electron, Scanning, mitosis inhibition, morphology, orthopedics, Osteoblasts, osteocalcin, Polystyrenes, Prostheses and Implants, prosthesis material, Tissue, tooth prosthesis},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032531299\&partnerID=40\&md5=5ea21d124ffce48018967f988cac815c},
	author = {Lopes, M.A. and Knowles, J.C. and Kuru, L. and Santos, J.D. and Monteiro, F.J. and Olsen, I.}
}
@article {2594,
	title = {In vitro biomineralization by osteoblast-like cells I. Retardation of tissue mineralization by metal salts},
	journal = {BiomaterialsBiomaterials},
	volume = {19},
	year = {1998},
	note = {Cited By (since 1996): 39Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(97)00149-XPubMed ID: 9678845Language of Original Document: EnglishCorrespondence Address: Carvalho, G.S.; IEC, Universidade do Minho, Largo do Paco, 4719 Braga Codex, PortugalChemicals/CAS: Alkaline Phosphatase, EC 3.1.3.1; Calcium, 7440-70-2; Cations; Chromium, 7440-47-3; Iron, 7439-89-6; Metals; Nickel, 7440-02-0; Phosphates; Stainless Steel, 12597-68-1; Tetrazolium Salts; Thiazoles; thiazolyl blue, 298-93-1References: Evans, E.M., Freeman, M.A.R., Miller, A.J., Vernon-Roberts, B., Metal sensitivity as a cause of bone necrosis and loosening of the prosthesis in total joint replacement (1974) J Bone Jt Surg, 56 B, pp. 626-642;Deutman, R., Rulder, T.J., Brian, R., Nater, J.P., Metal sensitivity before and after total hip arthroplasty (1977) J Bone Jt Surg, 59 A, pp. 862-865; 
Bartolozzi, A., Black, J., Chromium concentrations in serum, blood clot and urine from patients following total hip arthroplasty (1985) Biomaterials, 6, pp. 2-8; 
Dorr, L.D., Blocbaum, R., Emmanual, J., Meldrum, R., Histologic, biochemical and ion analysis of tissue and fluids retrieved during total hip arthroplasty (1990) Clin Orthop, 261, pp. 82-95; 
Henning, F.F., Raithel, H.J., Schaller, K.H., Dohler, J.R., Nickel-, chromium- and cobalt-concentrations in human tissue and body fluids of hip prosthesis patients (1992) J Trace Elem Electrolytes Health Dis, 6, pp. 239-243; 
Tom{\'a}s, H., Carvalho, G.S., Fernandes, M.H., Freire, A.P., Abrantes, L.M., Effects of Co-Cr corrosion products and corresponding separate metal ions on human osteoblast-like cell cultures (1996) J Mater Sci Mater Med, 7, pp. 291-296; 
Tom{\'a}s, H., Carvalho, G.S., Fernandes, M.H., Freire, A.P., Abrantes, L.M., The use of rat, rabbit or human bone marrow derived cells for cytocompatibility evaluation of metallic elements (1997) J Mater Sci Mater Med, 8, pp. 233-238; 
Pules, D.A., Huh, W.W., Acute toxicity of metal ions in cultures of osteogenic cells derived from bone marrow stromal cells (1995) J Appl Biomater, 6, pp. 109-116; 
Thompson, G.J., Pules, D.A., Effects of sublethal metal ion concentrations on osteogenic cells derived from bone marrow stromal cells (1995) J Appl Biomater, 6, pp. 249-258; 
McGee-Russell, S.M., Histochemical methods for calcium (1958) J Histochem Cytochem, 6, pp. 22-42; 
Bellows, C.G., Aubin, J.E., Heersche, J.N.M., Physiological concentrations of glucocorticoids stimulate formation of bone nodules from isolated rat calvaria cells in vitro (1987) Endocrinology, 121, pp. 1985-1992; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats (1988) Cell Tissue Res, 254, pp. 317-330; 
Wong, M., Rao, L.G., Li, H., Long-term effects of physiologic concentrations of dexamethasone on human bone derived cells (1990) J Bone Miner Res, 5, pp. 803-813; 
Aronow, M.A., Gerstenfeld, L.C., Owen, T.A., Factors that promote progressive development of the osteoblast phenotype in cultured fetal rat calvaria cells (1990) J Cell Phys, 143, pp. 213-221; 
Gotoh, Y., Hiraiwa, K., Narajama, M., In vitro mineralisation of osteoblastic cells derived from human bone (1990) Bone Miner, 8, pp. 239-250; 
Bellows, C.G., Aubin, J.E., Heersche, J.N.M., Initiation and progression of mineralisation of bone nodules formed in vitro: The role of alkaline phosphatase and organic phosphate (1991) Bone Miner, 14, pp. 27-40; 
Bellows, C.G., Heersche, J.N.M., Aubin, J.E., Inorganic phosphate added exogenously or released from β-glycerophosphate initiates mineralisation of osteoid nodules in vitro (1992) Bone Miner, 17, pp. 15-29; 
Chung, C.H., Golub, E.E., Forbes, E., Mechanisms of action of β-glycerophosphate on bone cell mineralisation (1992) Calcif Tissue Int, 51, pp. 305-311; 
Schimdt, R., Kulbe, K.D., Long term cultivation of human osteoblasts (1993) Bone Miner, 20, pp. 211-221; 
Cheng, S.L., Yang, J.W., Riffins, L., Differentiation of human bone marrow osteogenic stromal cells in vitro. Induction of the osteoblast phenotype by dexamethasone (1994) Endocrinology, 134, pp. 277-286; 
Gundle, R., Joyner, C.J., Triffit, J.T., Human bone tissue formation in diffusion chamber culture in vivo by bone derived cells and marrow stromal fibroblastic cells (1995) Bone, 16, pp. 597-601; 
Fernandes, M.H., Costa, M.A., Carvalho, G.S., Mineralisation in serially passaged human alveolar bone cells (1997) J Mater Sci Mater Med, 8, pp. 73-76; 
Martin, A., Clynes, M., Comparison of 5 microplate colorimetric assays for in vitro cytotoxicity testing and cell proliferation assays (1993) Cytotechnology, 11, pp. 49-58; 
Sgouras, D., Duncan, R., Methods for the evaluation of biocompatibility of soluble synthetic polymers which have potential for biomedical use. 1. Use of the tetrazolium-based colorimetric assay (MTT) as a preliminary screen for evaluation of in vitro cytotoxicity (1990) J Mater Sci Mater Med, 1, pp. 61-68; 
Ciapetti, G., Cenni, E., Stea, S., Pizzoferrato, A., In vitro evaluation of cell/biomatrial interaction by MTT assay (1993) Biomaterials, 14, pp. 359-364; 
Yoshimura, T., Kurita, C., Hayata, M., Nagai, H., Diagnosis of drug allergy by the lymphocyte stimulation test with the MTT (3-(4,5-dimethyl thiazol-2-yl)-2,2-diphenyl tetrazolium bromide) assay (1993) Biol Pharm Bull, 16, pp. 686-689; 
Sasanaluckit, P., Albustany, K.R., Doherty, P.J., Williams, D.F., Biocompatibility of glass ionomer cements (1993) Biomaterials, 14, pp. 906-916; 
Carvalho, G.S., Castanheira, M., Diogo, I., Inhibition and stimulation of enzymatic activities of human fibroblasts by corrosion products and metal salts (1996) J Mater Sci Mater Med, 7, pp. 77-83; 
Vrouwenvelder, W.C.A., Groot, C.G., De Groot, K., Histological and biochemical evaluation of osteoblasts cultured on bioactive glass, hydroxylapatite, titanium alloy and stainless steel (1993) J Biomed Mater Res, 27, pp. 465-475; 
Owen, M., Marrow stromal stem cells (1988) J Cell Sci, 10, pp. 63-76; 
Beresford, J.N., Osteogenic stem cells and the stromal system of bone and marrow (1989) Clin Orthop Rel Res, 240, pp. 270-280; 
Owen, T.A., Arohow, M., Shalhonb, V., Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation during formation of bone extracellular matrix (1990) J Cell Phys, 143, pp. 420-430; 
Malaval, L., Modrowski, D., Gupta, K., Aubin, J.E., Cellular expression of bone related proteins during in vitro osteogenesis in rat bone marrow stromal cell cultures (1994) J Cell Phys, 158, pp. 555-572; 
Stein, G.S., Lion, J.B., Molecular mechanisms mediated proliferation-differentiation interrelationships during progressive development of the osteoblast phenotype: Update 1995 (1995) End Rev, 4, pp. 290-297
},
	month = {1998///},
	pages = {13 - 21},
	publisher = {Elsevier Sci Ltd},
	abstract = {The cytocompatibility of stainless steel 316L (SS 316L) corrosion products was investigated with particular focus on the dose- and time-effect of electrochemically dissolved SS and the corresponding separate metal ions on osteogenic bone marrow derived cells. Type AISI 316L stainless steel (Fe 63.9\%, Cr 18.0\%, Ni 12.5\%, Mo 2.8\%, Si 1.2\%, Mn 1.6\% and C 0.025\%, weight for weight) was anodically dissolved in Hank{\textquoteright}s Balanced Salt Solution (HBSS) and diluted to the following concentrations: 500 μgml},
	keywords = {Absorption spectroscopy, alkaline phosphatase, animal cell, Animalia, Animals, article, ascorbic acid, atomic absorption spectrometry, balanced salt solution, Bioassay, Biocompatibility, biomaterial, Biomaterials, biomineralization, bone marrow cell, bone mineralization, Bovinae, Calcification, Physiologic, Calcium, calcium phosphate, Carbon, Cations, Cattle, Cell Division, cell proliferation, cell survival, cell viability, Cells, Cultured, Chromium, controlled study, Corrosion, dexamethasone, electrochemistry, enzyme activity, Enzyme kinetics, glycerol 2 phosphate, histochemistry, Iron, Kinetics, manganese, metal ion, Metal ions, Metals, molybdenum, Nickel, nonhuman, Oryctolagus cuniculus, osteoblast, Osteoblast cells, Osteoblast-like cells, Osteoblasts, Phosphates, priority journal, rabbit, Rabbits, serum, Silicon, Stainless steel, Steel corrosion, Tetrazolium Salts, Thiazoles, Tissue culture},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031812308\&partnerID=40\&md5=946525340a0c386c984ce614d56ae081},
	author = {Morais, S. and Sousa, J.P. and Fernandes, M.H. and Carvalho, G.S.}
}
@article {2582,
	title = {In vitro testing of surface-modified biomaterials},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {9},
	year = {1998},
	note = {Cited By (since 1996): 16Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1023/A:1008896106994Language of Original Document: EnglishCorrespondence Address: Leitao, E.; INEB-Instituto Engenharia Biomedica, FEUP-Faculdade de Engenharia, Universidade do Porto, Praca Coronel Pacheco 1, 4050 Porto, Portugal; email: eleitao@fsl.ho.man.ac.ukChemicals/CAS: calcium, 7440-70-2; carbon, 7440-44-0; nitrogen, 7727-37-9; stainless steel, 12597-68-1Tradenames: 316 L, aubert and duval; Thermanox; Ti-5Al-2.5Fe, deutsch titan; Ti-6Al-4V, deutsch titanManufacturers: aubert and duval; deutsch titanReferences: Kirkpatrick, C.J., Mittermayer, C., (1990) J. Mater. Sci. Mater. Med., 1, p. 9;Van Loon, J., (1995), PhD thesis, University of Leiden, The NetherlandsLi, P., De Groot, K., (1991) J. Mater. Sci. Mater. Med., 2, p. 125; 
Davies, J.E., (1990) Handbook of Bioactive Ceramics, 1, pp. 1-65. , edited by T. Yamamuro et al., CRC Press, Boca Raton, FL; 
Davies, J.E., Ottensmeyer, P., Shen, X., Hashimoto, M., Peel, S.A.F., (1991) Proceedings of the Bone Biomaterial Interface Workshop, pp. 214-228. , edited by J. E. Davies University of Toronto Press, Toronto; 
Gristina, A.G., Naylor, P., Myrvik, Q., (1988) Med. Progr. Through Technol., 14, p. 205; 
Kirkpatrick, C.J., Otterbach, T., Ander-Heiden, D., Schifer, J., Richter, H., H{\"o}cker, H., Mittermayer, C., Dekker, A., (1992) Cells Mater., 2, p. 169; 
Howlett, C.R., Evans, M.D.M., Wildish, K.L., Kelly, J.C., Fisher, L.R., Francis, G.W., Best, D.J., (1993) Clin. Mater., 14, p. 57; 
Howlett, C.R., Zreiqat, H., O{\textquoteright}Dell, R., Noorman, J., Evans, P., Dalton, B.A., McFarland, C., Steele, J.G., (1994) J. Mater. Sci. Mater. Med., 5, p. 715; 
Lee, J.-S., Kaibara, M., Iwaki, M., Sasabe, H., Suzuki, Y., Kusakabe, M., (1993) Biomaterials, 14, p. 958; 
R{\"o}stlund, T., Thomsen, P., Bjursten, L.M., Ericson, L.E., (1990) J. Biomed. Mater. Res., 24, p. 847; 
Johansson, C.B., Lausmaa, J., R{\"o}stlund, T., Thomsen, P., (1993) J. Mater. Sci. Mater. Med., 4, p. 132; 
Harmand, M.-F., Naji, A., Gonfrier, P., (1994) Clin. Mater., 15, p. 281; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., (1988) Cell Tissue Res., 254, p. 317; 
Vrouwenvelder, W.C.A., De Groot, C.G., De Groot, K., (1993) J. Biomed. Mater. Res., 27, p. 465; 
Puleo, D.A., Holleran, L.A., Doremus, R.H., Bizios, R., (1991) J. Biomed. Mater. Res., 25, p. 711; 
Davies, J.E., Lowenberger, B., Shiga, A., (1990) J. Biomed. Mater. Res., 24, p. 1289; 
De Bruijn, J.D., Klein, C.P.A.T., De Groot, K., Van Blitterswijk, C.A., (1993) Cells Mater., 3, p. 407; 
Gaillard, M.L., (1995), PhD thesis, University of Leiden, The NetherlandsRadder, A.M., Davies, J.E., Leenders, H., Van Blitterswijk, C.A., (1994) J. Biomed. Mater. Res., 28, p. 269; 
Shen, X., Roberts, E., Peel, S.A.F., Davies, J.F., (1993) Cells Mater., 3, p. 257; 
Gomi, K., Davies, J.E., (1993) J. Biomed. Mater. Res., 27, p. 429; 
De Bruijn, J.D., Klein, C.P.A.T., De Groot, K., Van Blitterswijk, C.A., (1992) J. Biomed. Mater. Res., 26, p. 1365; 
Orr, R.D., De Bruijn, J.D., Davies, J.E., (1992) Cells Mater., 2, p. 241
},
	month = {1998///},
	pages = {543 - 548},
	publisher = {Kluwer Academic Publishers},
	abstract = {The influence of surface modification treatments such as ion implantation and sputter coating on an in vitro rat bone-marrow cell culture was studied by scanning electron microscopy and X-ray microanalysis. 316 L stainless steel, Ti-6Al-4V and Ti-5Al-2.5Fe were nitrogen ion-implanted with three fluences: 10                        15, 10
                        16 and 10
                        17 ion cm
},
	keywords = {animal cell, animal tissue, article, biomaterial, Biomaterials, bone marrow culture, Calcium, Carbon, Cell culture, cell suspension, collagen fiber, extracellular matrix, implantation, in vitro study, In vitro testing, Ion implantation, male, materials testing, mineralization, morphology, nitrogen, nonhuman, priority journal, rat, Scanning electron microscopy, Sputtering, Stainless steel, Titanium alloys, Titanium aluminum iron alloy, Titanium aluminum vanadium alloy, X ray analysis, X ray microanalysis},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032170120\&partnerID=40\&md5=c39e91f7fc339fc95c225903e26f0ba5},
	author = {Leit{\~a}o, E. and Barbosa, M.A. and De Groot, K.}
}
@article {2596,
	title = {Guided bone regeneration using osteopatite{\textregistered} granules and polytetrafluoroethylene membranes},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {8},
	year = {1997},
	note = {Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1023/A:1018533217238Language of Original Document: EnglishCorrespondence Address: Vasconcelos, M.; Faculty of Dental Medicine, University of Porto, Rua Dr. Roberto Frias, 4200 Porto, PortugalTradenames: OsteopatiteReferences: Cavalheiro, J., Branco, R., Vasconcelos, M., (1991) Proceedings of the 4th International Conference on Bioceramics, p. 205. , edited by W. Bonfield, G. W. Hastings and K. E. Tanner, London, UK Butterworth-Heinemann, Oxford;Cavalheiro, J., Vasconcelos, M., Afonso, A., Peres, F., Branco, R., (1994) Stoma, 30, p. 15; 
Dahlin, C., Lekholm, U., Becker, W., Becker, B., Higuchi, K., Callens, A., Steenberghe, D., (1995) Int. J. Oral Maxillofac. Implants, 3, p. 312; 
Aughtun, M., Doz, P., Yildirim, M., Spiekermann, H., Biesterfeld, S., (1995) Int. J. Oral Maxillofac. Implants, 4, p. 421; 
Simion, M., Scarano, A., Gionso, L., Piattelli, A., (1995) Int. J. Oral Maxillofac. Implants, 3, p. 312; 
Afonso, A., Santos, J.D., Vasconcelos, M., Branco, R., Cavalheiro, J., (1996) J. Mater. Sci: Mater. Med., 7, p. 507; 
Jovanovic, S.A., Schenk, R.K., Orsini, M., Kenney, E.B., (1995) Int. J. Oral Maxillofac. Implants, 1, p. 27; 
Sander, L., Karring, T., (1995) J. Clin. Periodontol., 22, p. 295; 
Grevstad, H.J., Leknes, K.N., (1993) J. Clin. Periodontol., 20, p. 193; 
Piattelli, A., Scarano, A., Russo, P., Matarasso, S., (1996) Biomaterials, 17, p. 791
},
	month = {1997///},
	pages = {815 - 818},
	publisher = {Chapman \& Hall Ltd},
	abstract = {Granules of a modified hydroxyapatite, Osteopatite{\textregistered}, were implanted in the right posterior tibiae of adult rabbits. We studied the extent of bone regeneration in bone holes. In the right tibiae, that were filled with granules of this biomaterial covered with a polytetrafluoroethylene (PTFE) membrane using, as a control, uncovered granules. In the left tibia, an empty hole was covered with PTFE membrane and a second hole was left empty to be used as a control. A histomorphometric study was carried out using light microscopy, four and eight weeks after the surgery. The covered granules presented a higher percentage of bone contact than the uncovered ones, and it was also possible to observe a better bone tissue organization, mainly produced by the immobilization action of the PTFE membrane. Empty bone defects covered with PTFE membranes, two months after implantation, presented large areas of Haversian bone and direct bone contact to the PTFE membrane.Granules of a modified hydroxyapatite, Osteopatite, were implanted in the right posterior tibiae of adult rabbits. We studied the extent of bone regeneration in bone holes. In the right tibiae, that were filled with granules of this biomaterial covered with a polytetrafluoroethylene (PTFE) membrane using, as a control, uncovered granules. In the left tibia, an empty hole was covered with PTFE membrane and a second hole was left empty to be used as a control. A histomorphometric study was carried out using light microscopy, four and eight weeks after the surgery. The covered granules presented a higher percentage of bone contact than the uncovered ones, and it was also possible to observe a better bone tissue organization, mainly produced by the immobilization action of the PTFE membrane. Empty bone defects covered with PTFE membranes, two months after implantation, presented large areas of Haversian bone and direct bone contact to the PTFE membrane.
},
	keywords = {animal experiment, Animalia, biomaterial, Bone, bone defect, Bone Regeneration, conference paper, controlled study, haversian canal, hydroxyapatite, Implants (surgical), microscopy, nonhuman, Optical microscopy, Oryctolagus cuniculus, Phosphate minerals, politef, Polymeric membranes, Polytetrafluoroethylenes, priority journal, rabbit, tibia},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031438784\&partnerID=40\&md5=a60e39e457f8acd1d4a8b3586a52acf1},
	author = {Vasconcelos, M. and Afonso, A. and Branco, R. and Cavalheiro, J.}
}
@article {2603,
	title = {Influence of substrate material and surface finishing on the morphology of the calcium-phosphate coating},
	journal = {Journal of Biomedical Materials ResearchJ. BIOMED. MATER. RES.},
	volume = {36},
	year = {1997},
	note = {Cited By (since 1996): 11Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(199707)36:1<85::AID-JBM10>3.0.CO;2-IPubMed ID: 9212392Language of Original Document: EnglishCorrespondence Address: Leitao, E.; INEB, FEUP, Praca do Coronel Pacheco 1, 4050 Porto, PortugalChemicals/CAS: Alloys; alpha-tricalcium phosphate; Aluminum, 7429-90-5; Biocompatible Materials; calcium phosphate, 10103-46-5; Calcium Phosphates; dicalcium phosphate anhydrous, 7757-93-9; Hanks Balanced Salt Solution; Iron, 7439-89-6; Isotonic Solutions; monocalcium phosphate; Stainless Steel, 12597-68-1; tetracalcium phosphate; titanium alloy (TiAl6V4), 12743-70-3; Titanium, 7440-32-6References: Fujishiro, Y., Sato, T., Okuwaki, A., Coating of hydroxyapatite on metal plates using thermal dissociation of calcium-EDTA chelate in phosphate solutions under hydrothermal conditions (1995) Mater. Sci. Mater. Med., 6, pp. 172-176;Shirkhanzadeh, M., Calcium phosphate coatings prepared by electrocrystallization from aqueous electrolytes (1995) J. Mater, Sci. Mater. Med., 6, pp. 90-93; 
De Groot, K., Ceramics of calcium phosphates (1983) Bioceramics of Calcium Phosphates, p. 100. , K. de Groot (ed.), CRC Press, Boca Raton, FL; 
Leit{\~a}o, E., Barbosa, M.A., De Groot, K., In vitro calcification of orthopaedic implant materials (1995) J. Mater. Sci. Mater. Med., 6, pp. 849-852; 
Ducheyne, P., Radin, S., Ishikawa, K., The rate of calcium phosphate precipitation on metal and ceramics, and the relationship to bioactivity (1992) Bone Bonding Biomaterials, pp. 213-218. , P. Ducheyne, T. Kokubo, and C. A. van Blitterswijk (eds.), Reed Heathcare Communications, The Netherlands; 
Li, P., (1993) In Vitro and in Vivo Calcium Phosphate Induction on Gel Oxides, , Ph.D. thesis, Leiden University; 
Kokubo, T., Bioactivity of glasses and glass ceramics (1992) Bone Bonding Biomaterials, pp. 31-46. , P. Ducheyne, T. Kokubo, and C. A. van Blitterswijk (eds.), Reed Healthcare Communications, The Netherlands; 
Kokubo, T., Tanahashi, M., Yao, T., Minoda, M., Miyamoto, T., Nakamura, T., Yamamuro, T., Apatitepolymer composites prepared by biomimetic process: Improvement of adhesion of apatite to polymer by glow-discharge treatment (1993) Bioceramics, 6, pp. 327-332. , P. Ducheyne and D. Christiansen (eds.), Butterworth-Heinemann, Oxford, United Kingdom; 
Hanawa, T., Titanium and its oxide film: A substrate for forming apatite (1991) Proceedings of the Bone Biomaterial Interface Workshop, pp. 49-61. , Toronto, Dec., 1990, J. E. Davies (ed.), University of Toronto Press, Toronto, Canada; 
Hanawa, T., Ota, M., Calcium phosphate naturally formed on titanium in electrolyte solution (1991) Biomaterials, 12, pp. 767-774; 
Hanawa, T., Ota, M., Characterization of surface film formed on titanium in electrolyte using XPS (1992) Appl. Surface Sci., 55, pp. 269-276; 
Radin, S.R., Ducheyne, P., The effect of calcium phosphate ceramic composition and structure on in vivo behavior. II. Precipitation (1993) J. Biomed. Mater. Res., 27, pp. 35-45
},
	month = {1997///},
	pages = {85 - 90},
	publisher = {John Wiley \& Sons Inc},
	abstract = {The formation of an apatite-like layer was achieved by immersing Ti- 6A1-4V, Ti-A1-2.5Fe, and 316 L stainless-steel substrata in Hank{\textquoteright}s balanced salt solution (HBSS). The layer was characterized by surface analysis techniques, namely, X-ray microanalysis and X-ray diffraction, and the morphology was observed by scanning electron microscopy and atomic force microscopy. The concentrations of Ca and P were monitored as a function of time. The morphology of the precipitate layer seems to be dependent both on the type of metal substrate and its surface finish. Polished Ti-6A1-4V and Ti-A1-2.5Fe surfaces exhibit a plate precipitate morphology, whereas rougher surfaces show scattered crystal-like precipitation. The results suggest that the layer produced by immersion of polished titanium alloys in HBSS is constituted by an amorphous apatite.The formation of an apatite-like layer was achieved by immersing Ti-6Al-4V, Ti-Al-2.5Fe, and 316 L stainless-steel substrata in Hank{\textquoteright}s balanced salt solution (HBSS). The layer was characterized by surface analysis techniques, namely, X-ray microanalysis and X-ray diffraction, and the morphology was observed by scanning electron microscopy and atomic force microscopy. The concentrations of Ca and P were monitored as a function of time. The morphology of the precipitate layer seems to be dependent both on the type of metal substrate and its surface finish. Polished Ti-6Al-4V and Ti-Al-2.5Fe surfaces exhibit a plate precipitate morphology, whereas rougher surfaces show scattered crystal-like precipitation. The results suggest that the layer produced by immersion of polished titanium alloys in HBSS is constituted by an amorphous apatite.
},
	keywords = {Alloys, Aluminum, Aluminum alloys, article, Biocompatibility, Biocompatible Materials, biomaterial, Calcium Compounds, calcium phosphate, Calcium phosphate coatings, Calcium phosphates, Coatings, Finishing, Humans, hydroxyapatite, Implants (surgical), Iron, Isotonic Solutions, materials testing, metal implantation, Microscopy, Electron, Scanning, morphology, Phosphates, Stainless steel, Surface Properties, surface property, Surface testing, Surface treatment, Time Factors, Titanium, Titanium alloys, X ray diffraction, X ray diffraction analysis},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031195147\&partnerID=40\&md5=07a9f4ca9de83fbb3c66a25cfea71228},
	author = {Leit{\~a}o, E. and Barbosa, M.A. and De Groot, K.}
}
@article {2612,
	title = {Investigation of the dissolution of the bioceramic hydroxyapatite in the presence of titanium ions using ToF-SIMS and XPS},
	journal = {BiomaterialsBIOMATERIALS},
	volume = {18},
	year = {1997},
	note = {Cited By (since 1996): 28Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(96)00134-2PubMed ID: 9068892Language of Original Document: EnglishCorrespondence Address: Davies, M.C.; Lab. Biophysics and Surface Analysis, Department Pharmaceutical Sciences, University of Nottingham, Nottingham NG7 2RD, United KingdomChemicals/CAS: Biocompatible Materials; Cations; Ceramics; Durapatite, 1306-06-5; Titanium, 7440-32-6References: Hench, L.L., Wilson, J., Bioceramics (1991) MRS Bull, p. 62. , September;Katz, J.L., Harper, R.A., (1990) Concise Encyclopedia of Medical and Dental Materials, p. 87. , ed. D. F. Williams, Pergamon Press, Oxford; 
Posner, A.S., Betts, F., Synthetic amorphous calcium phosphate and its relation to bone mineral structure (1975) Acct Chem Res, 8, p. 2373; 
De Groot, K., (1981) Biocompatibility of Clinical Implant Materials. Vol. I, 1, p. 199. , ed. D. F. Williams, CRC Press, Boca Raton; 
Van Blitterswijk, C.A., Grote, J.J., Kuijpers, W., Daems, W.T., De Groot, K., Macropore tissue ingrowth: A quantitative and qualitative study on hydroxyapatite ceramic (1986) Biomaterials, 7, p. 137; 
Jarcho, M., Calcium phosphate ceramics as hard tissue prosthetics (1981) Clin Orthop Rel Res, 157, p. 259; 
De Groot, K., Geesink, R., Klein, C.P.A.T., Serekian, P., Plasma sprayed coatings of hydroxylapatite (1987) J Biomed Mater Res, 21, p. 1375; 
Kay, J.F., Golec, T.S., Riley, R.L., Hydroxyapatite coated sub-periosteal dental implants: Design rationale and clinical experience (1987) J Prosthet Dent, 58, p. 339; 
Geesink, R.G.T., De Groot, K.A., Klein, C.P.A.T., Chemical implant fixation using hydroxylapatite coatings: The development of a human total hip prosthesis for chemical fixation to bone using hydroxylapatite coatings on titanium substrates (1987) Clin Orthop Rel Res, 225, p. 147; 
Cook, S.D., Thomas, K.A., Kay, J.F., Jarcho, M., Hydroxyapatite coated titanium for orthopaedic implant applications (1988) Clin Orthop Rel Res, 232, p. 225; 
Van Blitterswijk, C.A., Grote, J.J., Kuijpers, Blok-Van Hoek, C.J.G., Daems, W.T., Bioreactions at the tissue/hydroxyapatite interface (1985) Biomaterials, 6, p. 243; 
De Lange, G.L.A., Donath, K., Interface between bone tissue and implants of solid hydroxyapatite-coated titanium implants (1989) Biomaterials, 10, p. 121; 
Craig, G.T., Brook, I.M., Lamb, D.J., Tissue response to subperiosteal implantation of dense hydroxyapatite: Case report (1989) Biomaterials, 10, p. 133; 
Lemons, J.E., Hydroxyapatite coatings (1988) Clin Orthop Rel Res, 235, p. 220; 
Van Blitterswijk, C.A., Grote, J.J., (1989) Critical Reviews in Biocompatibility, 5 (ISSUE 1), p. 13. , CRC Press; 
Driessens, F.C.M., (1983) Bioceramics of Calcium Phosphate, p. 1. , ed. K. de Groot, CRC Press, Boca Raton; 
Van Raemdonck, W., Ducheyne, P., DeMeester, P., (1984) Metal and Ceramic Biomaterials Vol. II, Strength and Surface, 2, p. 143. , eds P. Ducheyne and G. W. Hastings, CRC Press, Boca Raton; 
Lee, D.R., (1988) Dissolution Characterisation of Commercially Available Hydroxylapatite Particulate, , MSc thesis, University of Alabama at Birmingham; 
Whithead, R.Y., Lucas, L.C., Lacefield, W.R., The effect of dissolution on plasma sprayed hydroxylapatite coating on titanium (1993) Clin Materials, 12, p. 31; 
Driessens, F.C.M., Van Dijk, J.W.E., Borggreven, J.M.P.M., Biological calcium phosphates and their role in the physiology of bone and dental tissues I. Composition and solubility of calcium phosphates (1978) Calcif Tiss Res, 26, p. 127; 
LeGeros, R.Z., Tung, M.S., Chemical stability of carbonate and fluoride containing apatites (1983) Caries Research, 17, p. 419; 
Christoffersen, M.R., Thyregood, H.C., Christoffersen, J., Effects of aluminium (III), chromium (II), and iron (III) on the rate of dissolution of calcium hydroxyapatite crystals in the absence and presence of the chelating agent desferrioxamine (1987) J Calcif Tiss Int, 41, p. 27; 
Blumenthal, N.C., Cosma, V., Inhibition of apatic formation by titanium and vanadium ions (1989) J Biomed Mater Res, 23, p. 13; 
Ducheyne, P., Van Raemdonck, W., Heughebaert, J.C., Heughebaert, M., Structural analysis of hydroxyapatite coatings on titanium (1986) Biomaterials, 7, p. 97; 
Ellies, L.G., Nelson, D.G.A., Featherstone, J.D.B., Crystallographic structure and surface morphology of sintered carbonated apatites (1988) J Biomed Mater Res, 22, p. 541; 
Posner, A.S., The structure of bone apatite surfaces (1985) J Biomed Mater Res, 19, p. 241; 
Tudor, A.M., Davies, M.C., Melia, C.D., Anderson, D., Hastings, C., Santos, J.D., Barbosa, M., Morrey, S., The analysis of biomedical hydroxyapatite powders and hydroxyapatite coatings on metallic implant by near-IR Fourier transform Raman spectroscopy (1993) Spectrochimica Acta, 5-6, p. 675; 
Van Raemdonck, W., Ducheyne, P., De Meester, P., Auger electron spectroscopic analysis of hydroxyapatite coatings on titanium (1984) J Am Ceramic Soc, 67, p. 381; 
Smith, D.C., Murray, D.G., Zuccolin, J.D., Ruse, N.D., Surface characteristics of hydroxyapatite and adhesive bonding. 1. Surface characterisation (1987) J Adhesion, 22, p. 291; 
Meachim, G., Williams, D.F., (1973) J Biomed Mater Res, 13, p. 555; 
Pohler, O.E.M., (1983) Biomaterials in Reconstructive Surgery, p. 158. , ed. C. V. Mosby, London; 
Ribeiro, C.C., (1994), MSc. Thesis, University of PortoEccles, A.J., Vickerman, J.C., (1989) J Vac Sci Technol, A7, p. 234; 
Briggs, D., Hearn, M.J., Interaction of ion beams with polymer, with particular respect to SIMS (1986) Vacuum, 36, p. 1005; 
Ribeiro, C.C., Barbosa, M.A., Influence of metal ions on the dissolution behaviour of hydroxyapatite (1991) Bioceramics, 4, p. 145. , eds W. Bonfield, G. H. Hastings and K. E. Tanner, Butterworth-Heinemann; 
Reed, N.M., Vickerman, J.C., (1993) Surface Characterisation of Advanced Polymers, p. 83. , eds L. Sabbatini and P. G. Zambonin, Weinheim, VCH; 
Davies, M.C., Lynn, R.A.P., Paul, A.J., Vickerman, J.C., Heller, J., ToF-SIMS and XPS analysis of the surface chemical structure of some linear poly(ortho esters) (1991) Macromolecules, 24, p. 5508; 
Davies, M.C., Leadley, S.R., Paul, A.J., Vickerman, J.C., Heller, J., Franson, N.M., Probing the surface chemical structure of some novel poly(ortho-esters) prepared with N-methyl and N-phenyl ethanolamine by time-of-flight secondary ion mass spectrometry (ToF-SIMS) (1992) Polymers for Advanced Technologies, 3, pp. 293-301; 
Briggs, D., Ratner, B.D., A semi-quantitative SIMS analysis of the surfaces of random ethyl methacrylate:-Hydroxyethylmethacrylate copolymer films (1988) Polym Comm, 29, p. 6; 
Hearn, M.J., Ratner, B.D., Briggs, D., SIMS and XPS studies of poly(urethane) surfaces. I. Preliminary studies (1988) Macromolecules, 21, p. 2950; 
Briggs, D., Analysis of polymer surfaces by SIMS. VII. Homopolymer and copolymer nylons (1987) Org Mass Spectrom, 22, p. 91
},
	month = {1997///},
	pages = {311 - 316},
	publisher = {Elsevier Science Ltd},
	abstract = {Time-of-flight secondary ion mass spectrometry (ToF-SIMS) and X-ray photoelectron spectroscopy (XPS) analyses have been used to investigate the dissolution of hydroxyapatite in the presence of titanium chloride suggesting the substitution of titanium ions for calcium in the hydroxyapatite structure. The surface analytical data suggest that titanium was incorporated within the solid phase as titanium phosphate. Comparison of relative ion intensities in the ToF-SIMS spectra reveals changes in the composition of the surface chemistry of hydroxyapatite. These relative ion intensities show that the maximum surface uptake of titanium occurred at a solution concentration of 500 ppm titanium chloride for a time of incubation of 30 min. These data correlate with that obtained using XPS highlighting the semi-quantitative information which ToF-SIMS can provide.Time-of-flight secondary ion mass spectrometry (ToF-SIMS) and X-ray photoelectron spectroscopy (XPS) analyses have been used to investigate the dissolution of hydroxyapatite in the presence of titanium chloride suggesting the substitution of titanium ions for calcium in the hydroxyapatite structure. The surface analytical data suggest that titanium was incorporated within the solid phase as titanium phosphate. Comparison of relative ion intensities in the ToF-SIMS spectra reveals changes in the composition of the surface chemistry of hydroxyapatite. These relative ion intensities show that the maximum surface uptake of titanium occurred at a solution concentration of 500 ppm titanium chloride for a time of incubation of 30 min. These data correlate with that obtained using XPS highlighting the semi-quantitative information which ToF-SIMS can provide.
},
	keywords = {article, Bioceramic hydroxyapatite, Biocompatible Materials, biomaterial, Biomaterials, Cations, ceramic prosthesis, ceramics, Composition, Dissolution, Durapatite, hydroxyapatite, information processing, mass spectrometry, Phosphates, priority journal, Radiography, Secondary ion mass spectrometry, Spectrometry, Mass, Secondary Ion, Spectrometry, X-Ray Emission, Spectrophotometry, Substitution reactions, Surface chemistry, surface property, Surface structure, Time of flight secondary ion mass spectrometry, Time-of-flight secondary ion mass spectrometry, Titanium, Titanium chloride, X ray photoelectron spectroscopy, X-ray photoelectron spectroscopy},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0031080821\&partnerID=40\&md5=9581c063071547190645f666a211bddb},
	author = {Leadley, S.R. and Davies, M.C. and Ribeiro, C.C. and Barbosa, M.A. and Paul, A.J. and Watts, J.F.}
}
@article {2623,
	title = {In vitro calcium phosphate formation on SiO                    2-Na
                    2O-CaO-P
                    2O
                    5 glass reinforced hydroxyapatite composite: A study by XPS analysis
},
	journal = {Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED.},
	volume = {7},
	year = {1996},
	note = {Cited By (since 1996): 42Export Date: 21 November 2012Source: ScopusCODEN: JSMMELanguage of Original Document: EnglishCorrespondence Address: Santos, J.D.; FEUP, Rua dos Bragas, 4099-Porto Codex, PortugalChemicals/CAS: calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; hydroxyapatite, 1306-06-5, 51198-94-8References: Ducheyne, P., (1987) J. Biomed. Mater. Res.: Appl. Biomater., 21 (A2), p. 219;Hench, L.L., (1989) J. Biomed. Mater. Res., 23, p. 685; 
Hench, L.L., (1991) J. Amer. Ceram. Soc., 7, p. 1487; 
Jarcho, M., (1988) Bioceramics 1, p. 57. , edited by H. Oonishi, H. Aoki and K. Sawai Ishiyaku Euro America, Tokyo; 
De Groot, K., (1980) Biomaterials, 1, p. 47; 
Blitterswijk, C.A.V., Koerten, H.K., Grote, J.J., (1986) Biological and Biomechanical Performance of Biomaterials, p. 27. , edited by P. Cristel, A. Meunier and A. J. C. Lee Elsevier Science Publishers, Amsterdam; 
Ono, K., Yamamuro, T., Nakamura, T., Kokubo, T., Kotoura, Y., (1988) J. Biomed. Mater. Res., 22, p. 869; 
Aoki, H., (1991) Science and Medical Applications of Hydroxyapatite, , Japanese Association of Apatite Science (JAAS), Takayama Press System Centre Co., Inc; 
Manabe, A., Shigematsu, M., Kobayashi, S., (1987) High Tech Ceramics, p. 63. , edited by P. Vincenzini Elsevier Science Publishers, Amsterdam; 
Kitsugi, T., Yamamuro, T., Nakamura, T., Kokubo, T., Ono, M., (1987) J. Biomed. Mater. Res., 21, p. 1109; 
Kokubo, T., Ito, S., Shigematsu, M., Sakka, S., Yamamuro, T., (1985) J. Mater. Sci. Mater. Med., 20, p. 2001; 
Mestral, F., Drew, R.L.A., (1989) J. Eur. Ceram. Soc., 5, p. 47; 
Burnie, J., Gilchrist, T., Kobayashi, S., (1983) Ceramics in Surgery, p. 169. , edited by P. Vincenzini Elsevier Science Publishers, Amsterdam; 
Andersson, O.H., Liu, G., Karlsson, K.H., Niemi, L., Juhanoja, J., (1990) J. Mater. Sci. Mater. Med., 1, p. 219; 
Knowles, J.C., Rehman, I., Bonfield, W., (1994) Bioceramics 7, p. 85. , edited by O. H. Andersson, R. P. Happonen and A. Yli-Urpo Butterworth Heinemann, Turku, Finland; 
Santos, J.D., Reis, R.L., Knowles, J.C., Hastings, G.W., Monteiro, F.J., (1994) Biomaterials, 15, p. 5; 
Santos, J.D., Knowles, J.C., Monteiro, F.J., Hastings, G.W., (1992) Bioceramics 5, p. 35. , edited by T. Yamamuro, T. Kokubo and T. Nakamura Kokunshi Kankokai, Kyoto; 
Santos, J.D., Knowles, J.C., Reis, R.L., Monteiro, F.J., Hastings, G.W., (1985) J. Mater. Sci. Mater. Med., 6, p. 448; 
Wilson, J., Low, S., (1992) J. Appl. Biomater., 3, p. 123; 
Oonishi, H., Kushitani, S., Yasukawa, E., Hench, L.L., Wilson, J., Tsuji, E., Sugihara, T., (1994) Bioceramics 7, p. 139. , edited by O. H. Andersson, R.-P. Happonen and A. Yli-Urpo, Butterworth Heinemann, Turku, Finland; 
Neo, M., Nakamura, T., Yamamuro, T., Ohtsuki, C., Kokubo, T., Bando, Y., (1992) J. Biomed. Mater. Res., 26, p. 1419; 
Hyakuna, K., Yamamuro, T., Kotoura, Y., Kokubo, T., Kushitani, H., (1990) J. Biomed. Mater. Res., 24, p. 471; 
Kokubo, T., Kushitani, H., Sakka, S., Kitsugi, T., Yamamuro, T., (1990) J. Biomed. Mater. Res., 24, p. 721; 
Santos, J.D., Jha, L.J., Monteiro, F.J., (1995) Biomaterials, 16, p. 521; 
Li, P., Ohtsuki, C., Kokubo, T., Nakanishi, K., Soga, N., Nakamura, T., Yamamuro, T., (1993) J. Appl. Biomater., 4, p. 221; 
Neo, M., Kotani, S., Fujita, Y., Yamamuro, T., Bando, Y., Ohtsuki, C., Kokubo, T., (1992) J. Biomed. Mater. Res., 26, p. 255; 
Li, P., Yang, Q., Zhang, F., Kokubo, T., (1992) J. Mater. Sci. Mater. Med., 3, p. 452; 
Kokubo, T., Kushitani, H., Kitsugi, T., Yamamuro, T., (1988) Bioceramics 1, p. 157. , edited by T. Yamamuro, T. Kokubo and T. Nakamura Kokunshi Kankokai, Kyoto; 
Landis, W.J., Martin, J.R., (1984) J. Vac. Sci. Technol., A2, p. 1108; 
Andersson, O.H., Kangasniemi, I., (1991) J. Biomed. Mater. Res., 25, p. 1019; 
Verhoeven, J.A.T., Van Doveren, H., (1982) Surf. Sci., 123, p. 369; 
Rehman, I., Hench, L.L., Bonfield, W., (1994) Bioceramics 7, p. 123. , edited by O. H. Andersson, R. P. Happonen and A. Yli-Urpo Butterworth Heinemann, Turku, Finland; 
Turner, N.H., Murday, J.S., Ramaker, D.E., (1980) Anal. Chem., 52, p. 84
},
	month = {1996///},
	pages = {181 - 185},
	publisher = {Chapman \& Hall Ltd},
	abstract = {In-vitro apatite layer formation on the surface of a newly developed glass reinforced hydroxyapatite composite was characterized using X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) equipped with energy dispersive X-ray analysis (EDXA). After 1 week soaking in an acellular simulated body fluid the composite surface was entirely covered by a Ca, P-layer, suggesting a bioactive behaviour. XPS binding energy results revealed that this surface layer was a carbonated and non- stoichiometric apatite with Ca/P ratio of 1.3. This apatite layer was composed of very fine needle-like crystallites. Comparative studies on a commercially available hydroxyapatite showed that a similar apatite layer was also formed on its surface.In-vitro apatite layer formation on the surface of a newly developed glass reinforced hydroxyapatite composite was characterized using X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) equipped with energy dispersive X-ray analysis (EDXA). After 1 week soaking in an acellular simulated body fluid the composite surface was entirely covered by a Ca, P-layer, suggesting a bioactive behaviour. XPS binding energy results revealed that this surface layer was a carbonated and non-stoichiometric apatite with Ca/P ratio of 1.3. This apatite layer was composed of very fine needle-like crystallites. Comparative studies on a commercially available hydroxyapatite showed that a similar apatite layer was also formed on its surface.
},
	keywords = {article, Binding energy, Biocompatibility, Biodegradation, biomaterial, Calcium, calcium phosphate, Characterization, Composite materials, Composition, Energy dispersive x ray analysis, glass, Glass reinforced hydroxyapetite composite, hydroxyapatite, implant, In vitro calcium phosphate formation, Ionic concentration, molecular interaction, Nucleation, Phosphates, phosphorus, priority journal, Scanning electron microscopy, Surfaces, X ray analysis, X ray photoelectron spectroscopy},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0030106254\&partnerID=40\&md5=725eba3e273e23b5c6519d07483495e8},
	author = {Santos, J.D. and Jha, L.J. and Monteiro, F.J.}
}
@article {2636,
	title = {Stainless steel corrosion products cause alterations on mouse spleen cellular populations},
	journal = {Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED.},
	volume = {6},
	year = {1995},
	note = {Cited By (since 1996): 19Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/BF00121249Language of Original Document: EnglishCorrespondence Address: Tracana, R.B.; Natl. Inst. Biomedical Engineering, INEB, Praca Coronel Pacheco 1, 4000 Porto, Portugal},
	month = {1995///},
	pages = {56 - 61},
	publisher = {Chapman \& Hall Ltd},
	abstract = {Stainless steel is a metallic biomaterial commonly used in orthopaedic surgery. In this study we looked at the effects of stainless steel corrosion products on spleen, in order to evaluate their potential immunotoxicological effects. For this purpose stainless steel, type AISI 316L, was electrochemically dissolved in a physiological salt solution. The final solution, containing 490 μg/ml Fe, 224 μg/ml Cr and 150 μg/ml Ni, was injected subcutaneously in mice. After several periods of time (4, 10 and 14 days) spleens were removed and analysed for: (a) their contents in Fe, Cr and Ni, (b) histological alterations, (c) enumeration of cellular populations. Results showed that stainless steel corrosion products accumulated in spleen, caused histological alterations, and induced changes in cellular populations. When compared to chromium and nickel, iron was the metal ion preferentially accumulated in spleen. The increase of the multinucleated giant cell population was accompanied by depletion of lymphocyte populations. This study indicates that stainless steel corrosion products can spread systemically and accumulate in spleen inducing histological and cellular alterations which may give rise to immunotoxicological consequences.Stainless steel is a metallic biomaterial commonly used in orthopaedic surgery. In this study we looked at the effects of stainless steel corrosion products on spleen, in order to evaluate their potential immunotoxicological effects. For this purpose stainless steel, type AISI 316L, was electrochemically dissolved in a physiological salt solution. The final solution, containing 490 μg/ml Fe, 224 μg/ml Cr and 150 μg/ml Ni, was injected subcutaneously in mice. After several periods of time (4, 10 and 14 days) spleens were removed and analysed for: (a) their contents in Fe, Cr and Ni, (b) histological alterations, (c) enumeration of cellular populations. Results showed that stainless steel corrosion products accumulated in spleen, caused histological alterations, and induced changes in cellular populations. When compared to chromium and nickel, iron was the metal ion preferentially accumulated in spleen. The increase of the multinucleated giant cell population was accompanied by depletion of lymphocyte populations. This study indicates that stainless steel corrosion products can spread systematically and accumulate in spleen inducing histological and cellular alterations which may give rise to immunotoxicological consequences.
},
	keywords = {animal cell, animal experiment, animal tissue, Animalia, article, Biocompatibility, biomaterial, cell population, Cells, Chromium, Chronoamperometry, controlled study, Corrosion, Corrosion products, Dissolution, electrochemistry, giant cell, histology, immunology, immunotoxicity, Immunotoxicological effects, Ions, Iron, Lymphocyte, lymphocyte subpopulation, male, mouse, Nickel, nonhuman, orthopedic surgery, priority journal, sodium chloride, Spleen, spleen cell, Stainless steel, Steel corrosion, subcutaneous drug administration, toxicity},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0028883275\&partnerID=40\&md5=0d4737517419b9f7ccafa996ef5e1bb1},
	author = {Tracana, R.B. and Pereira, M.L. and Abreu, A.M. and Sousa, J.P. and Carvalho, G.S.}
}
@article {2639,
	title = {Stability of hydroxylapatite plasma-sprayed coated Ti-6Al-4V under cyclic bending in simulated physiological solutions},
	journal = {Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED.},
	volume = {5},
	year = {1994},
	note = {Cited By (since 1996): 35Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/BF00058983Language of Original Document: EnglishCorrespondence Address: Reis, R.L.; INEB-National Inst. Biomedical Engn., Faculty of Engineering, University of Porto, Rua dos Bragas, 4099 Porto Codex, Portugal},
	month = {1994///},
	pages = {457 - 462},
	publisher = {Chapman \& Hall Ltd},
	abstract = {The aim of this research was to study the stability of plasma-sprayed coated metal systems and to evaluate their susceptibility to the occurrence of corrosion fatigue. Hydroxylapatite plasma-sprayed coated samples of Ti-6Al-4V were studied under cyclic bending. During fatigue testing samples were immersed in a simulated physiological solution and mechanical and electrochemical degradation were monitored. Applied loads were intended to crack the ceramic coating and not the metal substrate. Electrochemical impedance spectroscopy was used to further characterize the electrochemical behaviour. No increase in tendency to corrode was detected in open-circuit corrosion fatigue testing. It appears as if the coating cracking does not increase metal substrate corrosion susceptibility. The coating integrity has been seriously affected, with marked decrease in thickness, due to the synergistic effect of load and presence of simulated body fluids environment. Impedance results, however, show a general tendency to an increase in corrosion kinetics after corrosion fatigue testing.The aim of this research was to study the stability of plasma-sprayed coated metal systems and to evaluate their susceptibility to the occurrence of corrosion fatigue. Hydroxylapatite plasma-sprayed coated samples of Ti-6Al-4V were studied under cyclic bending. During fatigue testing samples were immersed in a simulated physiological solution and mechanical and electrochemical degradation were monitored. Applied loads were intended to crack the ceramic coating and not the metal substrate. Electrochemical impedance spectroscopy was used to further characterize the electrochemical behaviour. No increase in tendency to corrode was detected in open-circuit corrosion fatigue testing. It appears as if the coating cracking does not increase metal substrate corrosion susceptibility. The coating integrity has been seriously affected, with marked decrease in thickness, due to the synergistic effect of load and presence of simulated body fluids environment. Impedance results, however, show a general tendency to an increase in corrosion kinetics after corrosion fatigue testing.
},
	keywords = {article, Bending (deformation), biomaterial, biomechanics, body fluid, Ceramic coatings, ceramics, controlled study, Corrosion, Corrosion fatigue, Corrosion susceptibility, Degradation, Electrochemical impedance spectroscopy, electrochemistry, fatigue, Fatigue testing, hydroxyapatite, impedance, mechanical stress, Physiological solutions, Plasma spraying, priority journal, sodium chloride, Solutions, Spectroscopy, Stability, Substrates, thickness, Titanium, Titanium alloys},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0028447410\&partnerID=40\&md5=f863751be3003b31534dd128601835f8},
	author = {Reis, R.L. and Monteiro, F.J. and Hastings, G.W.}
}
