



@article {3391,
	title = {Coffee silverskin: A possible valuable cosmetic ingredient},
	journal = {Pharmaceutical Biology},
	volume = {53},
	year = {2015},
	note = {Times Cited: 0},
	month = {2015},
	pages = {386-394},
	isbn = {1388-0209},
	url = {<Go to ISI>://WOS:000348831200011},
	author = {Rodrigues, Francisca and Palmeira-de-Oliveira, Ana and das Neves, Jose and Sarmento, Bruno and Helena Amaral, M. and Oliveira, M. Beatriz P. P.}
}
@article {3385,
	title = {Combined C4d and CD3 immunostaining predicts immunoglobulin (Ig)A nephropathy progression},
	journal = {Clinical and Experimental Immunology},
	volume = {179},
	year = {2015},
	note = {Times Cited: 0},
	month = {2015},
	pages = {354-361},
	isbn = {0009-9104},
	url = {<Go to ISI>://WOS:000347233800020},
	author = {Faria, B. and Henriques, C. and Matos, A. C. and Daha, M. R. and Pestana, M. and Seelen, M.}
}
@article {3383,
	title = {Cortical and trabecular bone in pediatric end-stage kidney disease},
	journal = {Pediatric Nephrology},
	volume = {30},
	year = {2015},
	month = {2015},
	pages = {497-502},
	isbn = {0931-041X},
	url = {<Go to ISI>://CCC:000349440700016},
	author = {Carvalho, Catarina G. and Pereira, Renata C. and Gales, Barbara and Salusky, Isidro B. and Wesseling-Perry, Katherine}
}
@article {3282,
	title = {Can artificial neural networks be used to predict the origin of ozone episodes?},
	journal = {Science of the Total Environment},
	volume = {488-489},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {197 - 207},
	abstract = {Tropospheric ozone is a secondary pollutant having a negative impact on health and environment. To control and minimize such impact the European Community established regulations to promote a clean air all over Europe. However, when an episode is related with natural mechanisms as Stratosphere-Troposphere Exchanges (STE), the benefits of an action plan to minimize precursor emissions are inefficient. Therefore, this work aims to develop a tool to identify the sources of ozone episodes in order to minimize misclassification and thus avoid the implementation of inappropriate air quality plans. For this purpose, an artificial neural network model - the Multilayer Perceptron - is used as a binary classifier of the source of an ozone episode. Long data series, between 2001 and 2010, considering the ozone precursors, 7Be activity and meteorological conditions were used. With this model, 2-7\% of a mean error was achieved, which is considered as a good generalization. Accuracy measures for imbalanced data are also discussed. The MCC values show a good performance of the model (0.65-0.92). Precision and F1-measure indicate that the model specifies a little better the rare class. Thus, the results demonstrate that such a tool can be used to help authorities in the management of ozone, namely when its thresholds are exceeded due natural causes, as the above mentioned STE. Therefore, the resources used to implement an action plan to minimize ozone precursors could be better managed avoiding the implementation of inappropriate measures. {\textcopyright} 2014 Elsevier B.V.},
	keywords = {artificial neural network, Classification, Human health, Ozone, Stratosphere, Troposphere},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84900308360\&partnerID=40\&md5=5bb79ec9cf75f45b1556274bf83ce812},
	author = {Fontes, T. and Silva, L.M. and Silva, M. P. and Barros, N. and Carvalho, A. C.}
}
@article {3272,
	title = {Cellular strategies to promote vascularisation in tissue engineering applications},
	journal = {European Cells and Materials},
	volume = {28},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {51 - 67},
	abstract = {Vascularisation is considered to be one of the greatest challenges in tissue engineering. Different strategies exist but cell-based approaches have emerged as a promising therapy to achieve successful vascularisation. The use of endothelial cells to engineer vascularised tissues has been extensively investigated. This field of research has evolved with the discovery of endothelial progenitor cells, a subpopulation with a high regenerative potential. However, the survival of endothelial cell populations alone seems to be impaired. To overcome this problem, co-culture systems, involving supporting cells, like mural cells, fibroblasts, or more tissue-specific cells have been developed. Endothelial cells benefit from the extracellular matrix components and growth factors produced by the supporting cells, which results in neovessel stabilisation and maturation. The use of endothelial progenitor cells in co-culture systems appears to be a promising strategy to promote vascularisation in approaches of increasing complexity. Herein, the authors provide an overview of the cellular strategies that can be used for increasing vascularisation in tissue engineering and regeneration.},
	keywords = {angiogenesis, Cellular therapies, Co-culture models, Endothelial progenitor cells, Regeneration},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84904957526\&partnerID=40\&md5=dbd4f3056e55c7dc52bc526ea7eb7d69},
	author = {Costa-Almeida, R. and Granja, P.L. and Soares, R. and Guerreiro, S.G.}
}
@article {3273,
	title = {Characterization of hLF1-11 immobilization onto chitosan ultrathin films, and its effects on antimicrobial activity},
	journal = {Acta Biomaterialia},
	volume = {10},
	year = {2014},
	note = {Cited By (since 1996):1Export Date: 21 November 2014},
	month = {2014},
	pages = {3513 - 3521},
	abstract = {hLF1-11 (GRRRRSVQWCA) is an antimicrobial peptide (AMP) with high activity against methicillin-resistant Staphylococcus aureus (MRSA), the most prevalent species in implant-associated infection. In this work, the effect of the surface immobilization on hLF1-11 antimicrobial activity was studied. Immobilization was performed onto chitosan thin films as a model for an implant coating due to its reported osteogenic and antibacterial properties. Chitosan thin films were produced by spin-coating on gold surfaces. hLF1-11 was immobilized onto these films by its C-terminal cysteine in an orientation that exposes the antimicrobial activity-related arginine-rich portion of the peptide. Two levels of exposure (with and without a polyethylene glycol (PEG) spacer) were analyzed. Covalent immobilization was further compared with the AMP physical adsorption onto chitosan films. Surfaces were characterized using ellipsometry, contact angle measurements, atomic force microscopy, infrared and X-ray photoelectron spectroscopies and using a fluorimetric assay for hLF1-11 quantification. Surface antimicrobial activity was assessed through surface adhesion and viability assays using an MRSA (S. aureus ATCC 33591). The incorporation of hLF1-11 increased significantly bacterial adhesion to chitosan films. However, the presence of hLF1-11, namely when immobilized through a PEG spacer, decreased the viability of adherent bacteria with regard to the control surface. These results demonstrated that hLF1-11 after covalent immobilization by its cysteine can maintain activity, particularly if a spacer is applied. However, further studies, exploring the opposite orientation or the same C-terminal orientation, but non-cysteine related, can help to clarify the potential of the hLF1-11 immobilization strategy. {\textcopyright} 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.},
	keywords = {Antimicrobial peptides (AMP), Bacterial adhesion, chitosan, Surface characterization, Surface immobilization},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84903724424\&partnerID=40\&md5=e8009cd480b49c37a991753cd145cf34},
	author = {Costa, F. and Maia, S. and Gomes, J. and Gomes, P. and Martins, M.C.L.}
}
@article {3300,
	title = {Chitosan cross-linked docetaxel loaded EGF receptor targeted nanoparticles for lung cancer cells},
	journal = {International Journal of Biological Macromolecules},
	volume = {69},
	year = {2014},
	note = {Cited By (since 1996):1Export Date: 21 November 2014},
	month = {2014},
	pages = {532 - 541},
	abstract = {Lung cancer, associated with the up-regulated epidermal growth factor receptor (EGFR) led to the development of EGFR targeted anticancer therapeutics. The biopolymeric nanoparticles form an outstanding system for the targeted delivery of therapeutic agents. The present work evaluated the in vitro effects of chitosan cross-linked γ-poly(glutamic acid) (γ-PGA) nanoparticles (Nps) loaded with docetaxel (DTXL) and decorated with Cetuximab (CET), targeted to EGFR over-expressing non-small-cell-lung-cancer (NSCLC) cells (A549). CET-DTXL-γ-PGA Nps was prepared by ionic gelation and CET conjugation via EDC/NHS chemistry. EGFR specificity of targeted Nps was confirmed by the higher uptake rates of EGFR +ve A549 cells compared to that of EGFR -ve cells (NIH3T3). The cytotoxicity of Nps quantified using cell based (MTT/LDH) and flowcytometry (Cell-cycle analysis, Annexin V/PI and JC-1) assays showed superior antiproliferative activity of CET-DTXL-γ-PGA Nps over DTXL-γ-PGA Nps. The A549 cells treated with CET-DTXL-γ-PGA NPs underwent a G2/M phase cell cycle arrest followed by reduction in mitochondrial membrane potential of A549 cells, inducing apoptosis and necrosis resulting in enhanced cancer cell death. CET-DTXL-γ-PGA Nps exhibited enhanced cellular internalization and therapeutic activity, by actively targeting EGFR on NSCLC cells and hence could be an effective alternative to non-specific, conventional chemotherapy by increasing its efficiency by many folds. {\textcopyright} 2014 Elsevier B.V.},
	keywords = {Cetuximab, chitosan, Epidermal growth factor receptor, Poly(γ-glutamic acid) nanoparticles, Targeted nanoparticles},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84903523289\&partnerID=40\&md5=728cbd3502e7922319f5f88fa8d6ec6d},
	author = {Maya, S. and Sarmento, B. and Lakshmanan, V.-K. and Menon, D. and Seabra, V. and Jayakumar, R.}
}
@article {3322,
	title = {Chitosan-modified porous silicon microparticles for enhanced permeability of insulin across intestinal cell monolayers},
	journal = {Biomaterials},
	volume = {35},
	year = {2014},
	note = {Cited By (since 1996):1Export Date: 21 November 2014},
	month = {2014},
	pages = {7172 - 7179},
	abstract = {Porous silicon (PSi) based particulate systems are emerging as an important drug delivery system due to its advantageous properties such as biocompatibility, biodegradability and ability to tailor the particles{\textquoteright} physicochemical properties. Here, annealed thermally hydrocarbonized PSi (AnnTHCPSi) and undecylenic acid modified AnnTHCPSi (AnnUnTHCPSi) microparticles were developed as a PSi-based platform for oral delivery of insulin. Chitosan (CS) was used to modify the AnnUnTHCPSi microparticles to enhance the intestinal permeation of insulin. Surface modification with CS led to significant increase in the interaction of PSi microparticles with Caco-2/HT-29 cell co-culture monolayers. Compared to pure insulin, the CS-conjugated microparticles significantly improved the permeation of insulin across the Caco-2/HT-29 cell monolayers, with ca. 20-fold increase in the amount of insulin permeated and ca. 7-fold increase in the apparent permeability (Papp) value. Moreover, among all the investigated particles, the CS-conjugated microparticles also showed the highest amount of insulin associated with the mucus layer and the intestinal Caco-2 cells and mucus secreting HT-29 cells. Our results demonstrate that CS-conjugated AnnUnTHCPSi microparticles can efficiently enhance the insulin absorption across intestinal cells, and thus, they are promising microsystems for the oral delivery of proteins and peptides across the intestinal cell membrane. {\textcopyright} 2014 Elsevier Ltd.},
	keywords = {Cell monolayers, chitosan, Diabetes, Insulin, Microparticles, Porous silicon},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84902079022\&partnerID=40\&md5=4de20e5572512006f72e11cb61a2035c},
	author = {Shrestha, N. and Shahbazi, M.-A. and Ara{\'u}jo, F. and Zhang, H. and M{\"a}kil{\"a}, E.M. and Kauppila, J. and Sarmento, B. and Salonen, J.J. and Hirvonen, J.T. and Santos, H.A.}
}
@article {3232,
	title = {Co-association of methotrexate and SPIONs into anti-CD64 antibody-conjugated PLGA nanoparticles for theranostic application},
	journal = {International Journal of Nanomedicine},
	volume = {9},
	year = {2014},
	month = {2014},
	pages = {4911-4922},
	url = {<Go to ISI>://WOS:000343406300001},
	author = {Moura, Catarina Costa and Segundo, Marcela A. and das Neves, Jose and Reis, Salette and Sarmento, Bruno}
}
@article {3325,
	title = {Collagen surface modified poly(ε-caprolactone) scaffolds with improved hydrophilicity and cell adhesion properties},
	journal = {Materials Letters},
	volume = {134},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {263 - 267},
	abstract = {Poly(ε-caprolactone) (PCL)/collagen scaffolds were produced using a four-step procedure comprising (i) the fabrication of PCL scaffolds through an additive bioextrusion system, (ii) the PCL surface modification using a plasma treatment, (iii) the acrylic acid (AAc) grafting by ultraviolet (UV) polymerization, and (iv) the immobilization of collagen into the scaffold surface through the carbodiimide chemistry. This procedure allowed the quick fabrication of highly porous scaffolds with interconnected pores, well-defined internal architecture and improved cell adhesion properties. The immobilization of collagen into the PCL surface led to a significant enhancement in the hydrophilicity, while the biological tests showed a further improvement in the adhesion of fibroblast cells. These results indicate that the collagen surface modification is an effective strategy to render the hydrophobic nature of PCL and overcome the limited cell interaction of 3D PCL scaffolds. {\textcopyright} 2014 Elsevier B.V.},
	keywords = {BioExtruder, Collagen, poly(ε-caprolactone), Scaffold, Surface modification},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84905669651\&partnerID=40\&md5=ebb38981b89fc93e5599a7592a37e134},
	author = {Sousa, I. and Mendes, A. and Pereira, R.F. and B{\'a}rtolo, P.J.}
}
@article {3400,
	title = {Complexity-loss in fetal heart rate dynamics during labor as a potential biomarker of acidemia},
	journal = {Early Human Development},
	volume = {90},
	year = {2014},
	note = {Times Cited: 1},
	month = {2014},
	pages = {67-71},
	isbn = {0378-3782},
	url = {<Go to ISI>://WOS:000331023300012},
	author = {Costa, Madalena D. and Schnettler, William T. and Amorim-Costa, Celia and Bernardes, Joao and Costa, Antonia and Goldberger, Ary L. and Ayres-de-Campos, Diogo}
}
@article {3411,
	title = {A critical appraisal of the evidence for using cardiotocography plus ECG ST interval analysis for fetal surveillance in labor. Part I: the randomized controlled trials},
	journal = {Acta Obstetricia Et Gynecologica Scandinavica},
	volume = {93},
	year = {2014},
	note = {Si},
	month = {2014},
	pages = {556-568},
	url = {<Go to ISI>://WOS:000337566200007},
	author = {Olofsson, Per and Ayres-de-Campos, Diogo and Kessler, Joerg and Tendal, Britta and Yli, Branka M. and Devoe, Lawrence}
}
@article {3274,
	title = {Crucial CD8+ T-lymphocyte cytotoxic role in amphotericin B nanospheres efficacy against experimental visceral leishmaniasis},
	journal = {Nanomedicine: Nanotechnology, Biology, and Medicine},
	volume = {10},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {1021 - 1030},
	abstract = {This work aims to develop poly(d,l-lactide-co-glycolide) (PLGA)-nanospheres containing amphotericin B (AmB) with suitable physicochemical properties and anti-parasitic activity for visceral leishmaniasis (VL) therapy. When compared with unloaded-PLGA-nanospheres, the AmB-loaded PLGA-nanospheres displayed an increased particle size without affecting the polydispersity and its negative surface charge. AmB stability in the PLGA-nanospheres was \&gt;90\% over 60-days at 30{\textdegree}C. The AmB-PLGA-nanospheres demonstrated significant in vitro and in vivo efficacy and preferential accumulation in the visceral organs. In addition, an immune-modulatory effect was observed in mice treated with AmB-PLGA-nanospheres, correlating with improved treatment efficacy. The in vitro cytotoxic response of the T-lymphocytes revealed that AmB-PLGA-nanospheres efficacy against VL infection was strictly due to the action of CD8+- but not CD4+-T lymphocytes. Overall, we demonstrate a crucial role for CD8+ cytotoxic T lymphocytes in the efficacy of AmB-PLGA nanospheres, which could represent a potent and affordable alternative for VL therapy. From the Clinical Editor: This study demonstrates a crucial role for CD8+ T lymphocytes in eliminating visceral leishmaniasis in a murine model by enhancing the cytotoxic efficacy of CD8+ T-cells via amphotericin-B-PLGA nanospheres, paving a way to a unique, potentially more potent and cost-effective therapeutic strategy. {\textcopyright} 2014 Elsevier Inc.},
	keywords = {Amphotericin B, Immune-modulation, Poly(d,l-lactide-co-glycolide) nanospheres, Visceral leishmaniasis},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84903548865\&partnerID=40\&md5=2258c0fa01cf6df10638a16d9368bed6},
	author = {Costa Lima, S.A. and Silvestre, R. and Barros, D. and Cunha, J. and Baltazar, M.T. and Dinis-Oliveira, R.J. and Cordeiro-da-Silva, A.}
}
@article {2869,
	title = {Cetuximab conjugated O-carboxymethyl chitosan nanoparticles for targeting EGFR overexpressing cancer cells},
	journal = {Carbohydrate Polymers},
	volume = {93},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {661 - 669},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84874136100\&partnerID=40\&md5=71075b389cbeb450f68caf7cbb271c08},
	author = {Maya, S. and Kumar, L.G. and Sarmento, B. and Sanoj Rejinold, N. and Menon, D. and Nair, S.V. and Jayakumar, R.}
}
@article {2888,
	title = {Characterization of human NLZ1/ZNF703 identifies conserved domains essential for proper subcellular localization and transcriptional repression},
	journal = {Journal of Cellular Biochemistry},
	volume = {114},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {120 - 133},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84869412286\&partnerID=40\&md5=e48a26e94d7ce0eddb65d8067fdc056c},
	author = {Pereira-Castro, I. and Costa, {\^A}.M.S. and Oliveira, M.J. and Barbosa, I. and Rocha, A.S. and Azevedo, L. and Da Costa, L.T.}
}
@article {2833,
	title = {Co-expression network analysis and genetic algorithms for gene prioritization in preeclampsia},
	journal = {BMC Medical Genomics},
	volume = {6},
	year = {2013},
	note = {Export Date: 12 December 2013Source: ScopusArt. No.: 51},
	month = {2013///},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84887314151\&partnerID=40\&md5=3daae7034430deb7d1e057fa432b236d},
	author = {Tejera, E. and Bernardes, J. and Rebelo, I.}
}
@article {2868,
	title = {Comparison of different methods of heart rate entropy analysis during acute anoxia superimposed on a chronic rat model of pulmonary hypertension},
	journal = {Medical Engineering and Physics},
	volume = {35},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {559 - 568},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84877632838\&partnerID=40\&md5=05c9ef6d6fea4a96d6a63f1749251066},
	author = {Gon{\c c}alves, H. and Henriques-Coelho, T. and Rocha, A.P. and Louren{\c c}o, A.P. and Leite-Moreira, A. and Bernardes, J.}
}
@article {2860,
	title = {Comparison of real beat-to-beat signals with commercially available 4 Hz sampling on the evaluation of foetal heart rate variability},
	journal = {Medical and Biological Engineering and Computing},
	volume = {51},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {665 - 676},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84877784836\&partnerID=40\&md5=cfdde94007fba73e03e51da6af74b995},
	author = {Gon{\c c}alves, H. and Costa, A. and Ayres-de-Campos, D. and Costa-Santos, C. and Rocha, A.P. and Bernardes, J.}
}
@article {2884,
	title = {Could moderate earthquakes also trigger cardiac events?},
	journal = {European Journal of Epidemiology},
	volume = {28},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {199 - 202},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84879501488\&partnerID=40\&md5=8ce97eb2baa2cb47b981c25b5f8fb023},
	author = {Ribeiro, A.I. and De F{\'a}tima De Pina, M.}
}
@article {2182,
	title = {Cancer syndromes and therapy by stop-codon readthrough},
	journal = {Trends in Molecular MedicineTrends Mol. Med.},
	volume = {18},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: TMMRCdoi: 10.1016/j.molmed.2012.09.004Language of Original Document: EnglishCorrespondence Address: Oliveira, C.; Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), 4200-465 Porto, Portugal; email: carlaol@ipatimup.ptReferences: Linde, L., Kerem, B., Introducing sense into nonsense in treatments of human genetic diseases (2008) Trends Genet., 24, pp. 552-563;Isken, O., Maquat, L.E., The multiple lives of NMD factors: balancing roles in gene and genome regulation (2008) Nat. Rev. Genet., 9, pp. 699-712; 
Lee, H.L., Dougherty, J.P., Pharmaceutical therapies to recode nonsense mutations in inherited diseases (2012) Pharmacol. Ther., 136, pp. 227-266; 
Stenson, P.D., The Human Gene Mutation Database: providing a comprehensive central mutation database for molecular diagnostics and personalized genomics (2009) Hum. Genomics, 4, pp. 69-72; 
Sloane, P.A., Rowe, S.M., Cystic fibrosis transmembrane conductance regulator protein repair as a therapeutic strategy in cystic fibrosis (2010) Curr. Opin. Pulm. Med., 16, pp. 591-597; 
Kerr, T.P., Long mutant dystrophins and variable phenotypes: evasion of nonsense-mediated decay? (2001) Hum. Genet., 109, pp. 402-407; 
Berger, A.H., A continuum model for tumour suppression (2011) Nature, 476, pp. 163-169; 
Oliveira, C., Quantification of epigenetic and genetic 2nd hits in CDH1 during hereditary diffuse gastric cancer syndrome progression (2009) Gastroenterology, 136, pp. 2137-2148; 
Schneikert, J., Behrens, J., The canonical Wnt signalling pathway and its APC partner in colon cancer development (2007) Gut, 56, pp. 417-425; 
van der Groep, P., Pathology of hereditary breast cancer (2011) Cell. Oncol. (Dordr.), 34, pp. 71-88; 
Oliveira, C., Germline CDH1 deletions in hereditary diffuse gastric cancer families (2009) Hum. Mol. Genet., 18, pp. 1545-1555; 
Fitzgerald, R.C., Hereditary diffuse gastric cancer: updated consensus guidelines for clinical management and directions for future research (2010) J. Med. Genet., 47, pp. 436-444; 
Fearon, E.R., Molecular genetics of colorectal cancer (2011) Annu. Rev. Pathol., 6, pp. 479-507; 
Beroud, C., Soussi, T., APC gene: database of germline and somatic mutations in human tumors and cell lines (1996) Nucleic Acids Res., 24, pp. 121-124; 
Minde, D.P., Messing up disorder: how do missense mutations in the tumor suppressor protein APC lead to cancer? (2011) Mol. Cancer, 10, p. 101; 
Half, E., Familial adenomatous polyposis (2009) Orphanet J. Rare Dis., 4, p. 22; 
Knudsen, A.L., Attenuated familial adenomatous polyposis: results from an international collaborative study (2010) Colorectal Dis., 12, pp. e243-e249; 
Papp, J., Germline MLH1 and MSH2 mutational spectrum including frequent large genomic aberrations in Hungarian hereditary non-polyposis colorectal cancer families: implications for genetic testing (2007) World J. Gastroenterol., 13, pp. 2727-2732; 
Marsh, D.J., Mutation spectrum and genotype-phenotype analyses in Cowden disease and Bannayan-Zonana syndrome, two hamartoma syndromes with germline PTEN mutation (1998) Hum. Mol. Genet., 7, pp. 507-515; 
Beggs, A.D., Peutz-Jeghers syndrome: a systematic review and recommendations for management (2010) Gut, 59, pp. 975-986; 
Amos, C.I., Genotype-phenotype correlations in Peutz-Jeghers syndrome (2004) J. Med. Genet., 41, pp. 327-333; 
Ognjanovic, S., Sarcomas in TP53 germline mutation carriers: a review of the IARC TP53 database (2012) Cancer, 118, pp. 1387-1396; 
Miki, Y., A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1 (1994) Science, 266, pp. 66-71; 
Goldberg, J.I., Borgen, P.I., Breast cancer susceptibility testing: past, present and future (2006) Expert Rev. Anticancer Ther., 6, pp. 1205-1214; 
Hakansson, S., Moderate frequency of BRCA1 and BRCA2 germ-line mutations in Scandinavian familial breast cancer (1997) Am. J. Hum. Genet., 60, pp. 1068-1078; 
Serova, O., A high incidence of BRCA1 mutations in 20 breast-ovarian cancer families (1996) Am. J. Hum. Genet., 58, pp. 42-51; 
Szabo, C.I., Understanding germ-line mutations in BRCA1 (2004) Cancer Biol. Ther., 3, pp. 515-520; 
Karam, R., The NMD mRNA surveillance pathway downregulates aberrant E-cadherin transcripts in gastric cancer cells and in CDH1 mutation carriers (2008) Oncogene, 27, pp. 4255-4260; 
Neu-Yilik, G., Mechanism of escape from nonsense-mediated mRNA decay of human beta-globin transcripts with nonsense mutations in the first exon (2011) RNA, 17, pp. 843-854; 
Slayton, R.L., Nonsense mutations in the COL1A1 gene preferentially reduce nuclear levels of mRNA but not hnRNA in osteogenesis imperfecta type I cell strains (2000) Matrix Biol., 19, pp. 1-9; 
Niyibizi, C., Gene therapy approaches for osteogenesis imperfecta (2004) Gene Ther., 11, pp. 408-416; 
Keeling, K.M., Bedwell, D.M., Suppression of nonsense mutations as a therapeutic approach to treat genetic diseases (2011) Wiley Interdiscip. Rev. RNA, 2, pp. 837-852; 
Perrin-Vidoz, L., The nonsense-mediated mRNA decay pathway triggers degradation of most BRCA1 mRNAs bearing premature termination codons (2002) Hum. Mol. Genet., 11, pp. 2805-2814; 
Fearnhead, N.S., The ABC of APC (2001) Hum. Mol. Genet., 10, pp. 721-733; 
Castellsague, E., Allele-specific expression of APC in adenomatous polyposis families (2010) Gastroenterology, 139, pp. 439-447. , 439-447, 447.e1; 
Kellermayer, R., Aminoglycosides as potential pharmacogenetic agents in the treatment of Hailey-Hailey disease (2006) J. Invest. Dermatol., 126, pp. 229-231; 
Allamand, V., Drug-induced readthrough of premature stop codons leads to the stabilization of laminin α2 chain mRNA in CMD myotubes (2008) J. Gene Med., 10, pp. 217-224; 
Welch, E.M., PTC124 targets genetic disorders caused by nonsense mutations (2007) Nature, 447, pp. 87-91; 
Carnes, J., Suppression of eukaryotic translation termination by selected RNAs (2000) RNA, 6, pp. 1468-1479; 
Carnes, J., Stop codon suppression via inhibition of eRF1 expression (2003) RNA, 9, pp. 648-653; 
Capone, J.P., Amber, ochre and opal suppressor tRNA genes derived from a human serine tRNA gene (1985) EMBO J., 4, pp. 213-221; 
Nakamura, A., Takeda, S., Mammalian models of Duchenne Muscular Dystrophy: pathological characteristics and therapeutic applications (2011) J. Biomed. Biotechnol., 2011, p. 184393; 
Linde, L., Nonsense-mediated mRNA decay affects nonsense transcript levels and governs response of cystic fibrosis patients to gentamicin (2007) J. Clin. Invest., 117, pp. 683-692; 
Linde, L., The efficiency of nonsense-mediated mRNA decay is an inherent character and varies among different cells (2007) Eur. J. Hum. Genet., 15, pp. 1156-1162; 
Keeling, K.M., Leaky termination at premature stop codons antagonizes nonsense-mediated mRNA decay in S. cerevisiae (2004) RNA, 10, pp. 691-703; 
Sako, Y., A novel therapeutic approach for genetic diseases by introduction of suppressor tRNA (2006) Nucleic Acids Symp. Ser. (Oxf.), 50, pp. 239-240; 
Hainrichson, M., Designer aminoglycosides: the race to develop improved antibiotics and compounds for the treatment of human genetic diseases (2008) Org. Biomol. Chem., 6, pp. 227-239; 
Kellermayer, R., Translational readthrough induction of pathogenic nonsense mutations (2006) Eur. J. Med. Genet., 49, pp. 445-450; 
Heier, C.R., DiDonato, C.J., Translational readthrough by the aminoglycoside geneticin (G418) modulates SMN stability in vitro and improves motor function in SMA mice in vivo (2009) Hum. Mol. Genet., 18, pp. 1310-1322; 
Arakawa, M., Negamycin restores dystrophin expression in skeletal and cardiac muscles of mdx mice (2003) J. Biochem., 134, pp. 751-758; 
Nudelman, I., Repairing faulty genes by aminoglycosides: development of new derivatives of geneticin (G418) with enhanced suppression of diseases-causing nonsense mutations (2010) Bioorg. Med. Chem., 18, pp. 3735-3746; 
Nudelman, I., Development of novel aminoglycoside (NB54) with reduced toxicity and enhanced suppression of disease-causing premature stop mutations (2009) J. Med. Chem., 52, pp. 2836-2845; 
Du, M., PTC124 is an orally bioavailable compound that promotes suppression of the human CFTR-G542X nonsense allele in a CF mouse model (2008) Proc. Natl. Acad. Sci. U.S.A., 105, pp. 2064-2069; 
Hirawat, S., Safety, tolerability, and pharmacokinetics of PTC124, a nonaminoglycoside nonsense mutation suppressor, following single- and multiple-dose administration to healthy male and female adult volunteers (2007) J. Clin. Pharmacol., 47, pp. 430-444; 
Teng, S., Readthrough of nonsense mutation W822X in the SCN5A gene can effectively restore expression of cardiac Na+ channels (2009) Cardiovasc. Res., 83, pp. 473-480; 
Diop, D., Aminoglycosides and other factors promoting stop codon readthrough in human cells (2007) C. R. Biol., 330, pp. 71-79; 
Buvoli, M., Suppression of nonsense mutations in cell culture and mice by multimerized suppressor tRNA genes (2000) Mol. Cell. Biol., 20, pp. 3116-3124; 
von der Haar, T., Tuite, M.F., Regulated translational bypass of stop codons in yeast (2007) Trends Microbiol., 15, pp. 78-86; 
Rodriguez, E.A., In vivo incorporation of multiple unnatural amino acids through nonsense and frameshift suppression (2006) Proc. Natl. Acad. Sci. U.S.A., 103, pp. 8650-8655; 
Panchal, R.G., Partial functional correction of xeroderma pigmentosum group A cells by suppressor tRNA (1999) Hum. Gene Ther., 10, pp. 2209-2219; 
Bhuvanagiri, M., NMD: RNA biology meets human genetic medicine (2010) Biochem. J., 430, pp. 365-377; 
Gubbens, J., In vitro incorporation of nonnatural amino acids into protein using tRNA(Cys)-derived opal, ochre, and amber suppressor tRNAs (2010) RNA, 16, pp. 1660-1672; 
Amrani, N., A faux 3{\textquoteright}-UTR promotes aberrant termination and triggers nonsense-mediated mRNA decay (2004) Nature, 432, pp. 112-118; 
Mintzer, M.A., Simanek, E.E., Nonviral vectors for gene delivery (2009) Chem. Rev., 109, pp. 259-302; 
Oliveira, H., Targeted gene delivery into peripheral sensorial neurons mediated by self-assembled vectors composed of poly(ethylene imine) and tetanus toxin fragment c (2010) J. Control. Release, 143, pp. 350-358; 
Duncan, R., Gaspar, R., Nanomedicine(s) under the microscope (2011) Mol. Pharm., 8, pp. 2101-2141; 
Zilberberg, A., Restoration of APC gene function in colorectal cancer cells by aminoglycoside- and macrolide-induced read-through of premature termination codons (2010) Gut, 59, pp. 496-507; 
Keeling, K.M., Bedwell, D.M., Clinically relevant aminoglycosides can suppress disease-associated premature stop mutations in the IDUA and P53 cDNAs in a mammalian translation system (2002) J. Mol. Med. (Berl.), 80, pp. 367-376; 
Floquet, C., Rescue of non-sense mutated p53 tumor suppressor gene by aminoglycosides (2011) Nucleic Acids Res., 39, pp. 3350-3362; 
Simoes-Correia, J., Endoplasmic reticulum quality control: a new mechanism of E-cadherin regulation and its implication in cancer (2008) Hum. Mol. Genet., 17, pp. 3566-3576; 
Mateus, A.R., E-cadherin mutations and cell motility: a genotype-phenotype correlation (2009) Exp. Cell Res., 315, pp. 1393-1402; 
Figueiredo, J., ADP-ribosylation factor 6 mediates E-cadherin recovery by chemical chaperones (2011) PLoS ONE, 6, pp. e23188; 
Demeshkina, N., A new understanding of the decoding principle on the ribosome (2012) Nature, 484, pp. 256-259; 
Wagner, K.R., Gentamicin treatment of Duchenne and Becker muscular dystrophy due to nonsense mutations (2001) Ann. Neurol., 49, pp. 706-711; 
Politano, L., Gentamicin administration in Duchenne patients with premature stop codon. Preliminary results (2003) Acta Myol., 22, pp. 15-21; 
Malik, V., Gentamicin-induced readthrough of stop codons in Duchenne muscular dystrophy (2010) Ann. Neurol., 67, pp. 771-780; 
Wilschanski, M., A pilot study of the effect of gentamicin on nasal potential difference measurements in cystic fibrosis patients carrying stop mutations (2000) Am. J. Respir. Crit. Care Med., 161, pp. 860-865; 
Clancy, J.P., Evidence that systemic gentamicin suppresses premature stop mutations in patients with cystic fibrosis (2001) Am. J. Respir. Crit. Care Med., 163, pp. 1683-1692; 
Wilschanski, M., Gentamicin-induced correction of CFTR function in patients with cystic fibrosis and CFTR stop mutations (2003) N. Engl. J. Med., 349, pp. 1433-1441; 
Clancy, J.P., No detectable improvements in cystic fibrosis transmembrane conductance regulator by nasal aminoglycosides in patients with cystic fibrosis with stop mutations (2007) Am. J. Respir. Cell Mol. Biol., 37, pp. 57-66; 
Sermet-Gaudelus, I., In vitro prediction of stop-codon suppression by intravenous gentamicin in patients with cystic fibrosis: a pilot study (2007) BMC Med., 5, p. 5; 
Kerem, E., Effectiveness of PTC124 treatment of cystic fibrosis caused by nonsense mutations: a prospective phase II trial (2008) Lancet, 372, pp. 719-727; 
Sermet-Gaudelus, I., Ataluren (PTC124) induces cystic fibrosis transmembrane conductance regulator protein expression and activity in children with nonsense mutation cystic fibrosis (2010) Am. J. Respir. Crit. Care Med., 182, pp. 1262-1272; 
Wilschanski, M., Chronic ataluren (PTC124) treatment of nonsense mutation cystic fibrosis (2011) Eur. Respir. J., 38, pp. 59-69; 
James, P.D., Aminoglycoside suppression of nonsense mutations in severe hemophilia (2005) Blood, 106, pp. 3043-3048; 
Pinotti, M., Gentamicin induces sub-therapeutic levels of coagulation factor VII in patients with nonsense mutations (2006) J. Thromb. Haemost., 4, pp. 1828-1830; 
Schroers, A., Gentamicin treatment in McArdle disease: failure to correct myophosphorylase deficiency (2006) Neurology, 66, pp. 285-286; 
Simon, A.J., The effect of gentamicin-induced readthrough on a novel premature termination codon of CD18 leukocyte adhesion deficiency patients (2010) PLoS ONE, 5, pp. e13659
},
	month = {2012///},
	pages = {667 - 678},
	abstract = {Several hereditary cancer syndromes are associated with nonsense mutations that create premature termination codons (PTC). Therapeutic strategies involving readthrough induction partially restore expression of proteins with normal function from nonsense-mutated genes, and small molecules such as aminoglycosides and PTC124 have exhibited promising results for treating patients with cystic fibrosis and Duchenne muscular dystrophy. Transgenic expression of suppressor-tRNAs and depleting translation termination factors are, among others, potential strategies for treating PTC-associated diseases. In this review, the potential of using readthrough strategies as a therapy for cancer syndromes is discussed, and we consider the effect of nonsense-mediated decay and other factors on readthrough efficiency. {\textcopyright} 2012 Elsevier Ltd.},
	keywords = {Aminoglycosides, Hereditary cancer syndromes, Nonsense-mediated decay (NMD), Nonsense-suppression, Premature termination codon (PTC), PTC124, Readthrough, Suppressor-tRNA},
	isbn = {14714914 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84868212770\&partnerID=40\&md5=93ff561cb7d1c818f78a88ec1555181e},
	author = {Bordeira-Carri{\c c}o, R. and P{\^e}go, A.P. and Santos, M. and Oliveira, C.}
}
@article {2209,
	title = {Cell-based in vitro models for predicting drug permeability},
	journal = {Expert Opinion on Drug Metabolism and ToxicologyExpert Opin. Drug Metab. Toxicol.},
	volume = {8},
	year = {2012},
	note = {Export Date: 21 November 2012Source: Scopusdoi: 10.1517/17425255.2012.673586PubMed ID: 22424145Language of Original Document: EnglishCorrespondence Address: Sarmento, B.; CICS, Department of Pharmaceutical Sciences, Instituto Superior de Ci{\^e}ncias da Sa{\'u}de - Norte, Gandra, Portugal; email: bruno.sarmento@ff.up.ptChemicals/CAS: Pharmaceutical PreparationsReferences: Polli, J., In vitro studies are sometimes better than conventional human pharmacokinetic in vivo studies in assessing bioequivalence of immediate-release solid oral dosage forms (2008) AAPS J., 10, pp. 289-299;Pelkonen, O., Boobis, A.R., Gundert-Remy, U., In vitro prediction of gastrointestinal absorption and bioavailability: An experts{\textquoteright} meeting report (2001) Eur. J. Clin. Pharmacol., 57, pp. 621-629; 
Lu, Y., Kim, S., Park, K., In vitro-in vivo correlation: Perspectives on model development (2011) Int. J. Pharm., 418, pp. 142-148; 
Balimane, P.V., Chong, S., Morrison, R.A., Current methodologies used for evaluation of intestinal permeability and absorption (2000) Journal of Pharmacological and Toxicological Methods, 44 (1), pp. 301-312. , DOI 10.1016/S1056-8719(00)00113-1, PII S1056871900001131; 
Antunes, F., Andrade, F., Ferreira, D., Models to predict intestinal absorption of therapeutic peptides and proteins Curr. Drug. Metab., , In press; 
Fearn, R.A., Hirst, B.H., Predicting oral drug absorption and hepatobiliary clearance: Human intestinal and hepatic in vitro cell models (2006) Environmental Toxicology and Pharmacology, 21 (SPEC. ISS. 2), pp. 168-178. , DOI 10.1016/j.etap.2005.06.002, PII S1382668905001092; 
Shah, A.K., Agnihotri, S.A., Recent advances and novel strategies in pre-clinical formulation development: An overview (2011) J. Control. Release, 156, pp. 281-296; 
Balimane, P.V., Han, Y.H., Chong, S., Current industrial practices of assessing permeability and P-glycoprotein interaction (2006) AAPS J., 8, pp. E1-E13; 
Martinez, M.N., Amidon, G.L., A mechanistic approach to understanding the factors affecting drug absorption: A review of fundamentals (2002) Journal of Clinical Pharmacology, 42 (6), pp. 620-643. , DOI 10.1177/00970002042006005; 
Sugano, K., Kansy, M., Artursson, P., Coexistence of passive and carrier-mediated processes in drug transport (2010) Nat. Rev. Drug. Discov., 9, pp. 597-614; 
Bohets, H., Annaert, P., Mannens, G., Strategies for absorption screening in drug discovery and development (2001) Curr. Top. Med. Chem., 1, pp. 367-383; 
Rubas, W., Cromwell, M.E.M., Shahrokh, Z., Villagran, J., Nguyen, T.-N., Wellton, M., Nguyen, T.-H., Mrsny, R.J., Flux measurements across Caco-2 monolayers may predict transport in human large intestinal tissue (1996) Journal of Pharmaceutical Sciences, 85 (2), pp. 165-169. , DOI 10.1021/js950267+; 
Artursson, P., Palm, K., Luthman, K., Caco-2 monolayers in experimental and theoretical predictions of drug transport (2001) Adv. Drug. Deliv. Rev., 46, pp. 27-43; 
Sambuy, Y., De Angelis, I., Ranaldi, G., Scarino, M.L., Stammati, A., Zucco, F., The Caco-2 cell line as a model of the intestinal barrier: Influence of cell and culture-related factors on Caco-2 cell functional characteristics (2005) Cell Biology and Toxicology, 21 (1), pp. 1-26. , DOI 10.1007/s10565-005-0085-6; 
Gres, M.-C., Julian, B., Bourrie, M., Meunier, V., Roques, C., Berger, M., Boulenc, X., Fabre, G., Correlation between oral drug absorption in humans, and apparent drug permeability in TC-7 cells, A human epithelial intestinal cell line: Comparison with the parental Caco-2 cell line (1998) Pharmaceutical Research, 15 (5), pp. 726-733. , DOI 10.1023/A:1011919003030; 
Da Violante, G., Zerrouk, N., Richard, I., Frendo, J.-L., Zhiri, A., Li-Khuan, R., Tricottet, V., Arnaud, P., Short term Caco-2/TC7 cell culture: Comparison between of conventional 21-d and a commercially available 3-d system (2004) Biological and Pharmaceutical Bulletin, 27 (12), pp. 1986-1992. , http://www.jstage.jst.go.jp/article/bpb/27/12/1986/_pdf, DOI 10.1248/bpb.27.1986; 
Dahiya, R., Lesuffleur, T., Kwak, K.S., Expression and characterization of mucins associated with the resistance to methotrexate of human colonic adenocarcinoma cell line HT29 (1992) Cancer Res., 52, pp. 4655-4662; 
Lesuffleur, T., Violette, S., Vasile-Pandrea, I., Dussaulx, E., Barbat, A., Muleris, M., Zweibaum, A., Resistance to high concentrations of methotrexate and 5-fluorouracil of differentiated HT-29 colon-cancer cells is restricted to cells of enterocytic phenotype (1998) International Journal of Cancer, 76 (3), pp. 383-392. , DOI 10.1002/(SICI)1097-0215(19980504)76:3<383::AID-IJC16>3.0.CO;2-C; 
Behrens, I., Stenberg, P., Artursson, P., Kissel, T., Transport of lipophilic drug molecules in a new mucus-secreting cell culture model based on HT29-MTX cells (2001) Pharmaceutical Research, 18 (8), pp. 1138-1145. , DOI 10.1023/A:1010974909998; 
Wikman-Larhed, A., Artursson, P., Co-cultures of human intestinal goblet (HT29-H) and absorptive (Caco-2) cells for studies of drug and peptide absorption (1995) Eur. J. Pharm. Sci., 3, pp. 171-183; 
Hilgendorf, C., Spahn-Langguth, H., Regardh, C.G., Lipka, E., Amidon, G.L., Langguth, P., Caco-2 versus Caco-2/HT29-MTX co-cultured cell lines: Permeabilities via diffusion, inside- and outside-directed carrier-mediated transport (2000) Journal of Pharmaceutical Sciences, 89 (1), pp. 63-75. , DOI 10.1002/(SICI)1520-6017(200001)89:1<63::AID-JPS7>3.0.CO;2-6; 
Versantvoort, C.H.M., Ondrewater, R.C.A., Duizer, E., Van De Sandt, J.J.M., Gilde, A.J., Groten, J.P., Monolayers of IEC-18 cells as an in vitro model for screening the passive transcellular and paracellular transport across the intestinal barrier: Comparison of active and passive transport with the human colon carcinoma Caco-2 cell line (2002) Environmental Toxicology and Pharmacology, 11 (3-4), pp. 335-344. , DOI 10.1016/S1382-6689(01)00122-3, PII S1382668901001223; 
Steensma, A., Noteborn, H.P.J.M., Kuiper, H.A., Comparison of Caco-2, IEC-18 and HCEC cell lines as a model for intestinal absorption of genistein, daidzein and their glycosides (2004) Environmental Toxicology and Pharmacology, 16 (3), pp. 131-139. , DOI 10.1016/j.etap.2003.11.008, PII S1382668903001091; 
Coon, S., Kekuda, R., Saha, P., Sundaram, U., Reciprocal regulation of the primary sodium absorptive pathways in rat intestinal epithelial cells (2011) Am. J. Physiol. Cell Physiol., 300, pp. C496-C505; 
Cho, M.J., Thompson, D.P., Cramer, C.T., Vidmar, T.J., Scieszka, J.F., The Madin Darby canine kidney (MDCK) epithelial cell monolayer as a model cellular transport barrier (1989) Pharmaceutical Research, 6 (1), pp. 71-77. , DOI 10.1023/A:1015807904558; 
Avdeef, A., Tam, K.Y., How well can the caco-2/Madin-darby canine kidney models predict effective human jejunal permeability (2010) J. Med. Chem., 53, pp. 3566-3584; 
Deferme, S., Annaert, P., Augustijns, P., In vitro screening models to assess intestinal drug absorption and metabolism (2008) Drug Absorption Studies, , Kim CEaK-J, editor. Springer; US:; 
Walter, E., Janich, S., Roessler, B.J., Hilfinger, J.M., Amidon, G.L., HT29-MTX/Caco-2 cocultures as an in vitro model for the intestinal epithelium: In vitro-in vivo correlation with permeability data from rats and humans (1996) Journal of Pharmaceutical Sciences, 85 (10), pp. 1070-1076. , DOI 10.1021/js960110x; 
Woitiski, C.B., Sarmento, B., Carvalho, R.A., Facilitated nanoscale delivery of insulin across intestinal membrane models (2011) Int. J. Pharm., 412, pp. 123-131; 
Fonte, P., Nogueira, T., Gehm, C., Chitosan-coated solid lipid nanoparticles enhance the oral absorption of insulin (2011) Drug. Deliv. Transl. Res., 1, pp. 299-308; 
Gullberg, E., Leonard, M., Karlsson, J., Hopkins, A.M., Brayden, D., Baird, A.W., Artursson, P., Expression of specific markers and particle transport in a new human intestinal M-cell model (2000) Biochemical and Biophysical Research Communications, 279 (3), pp. 808-813. , DOI 10.1006/bbrc.2000.4038; 
Lo, D., Tynan, W., Dickerson, J., Scharf, M., Cooper, J., Byrne, D., Brayden, D., O{\textquoteright}Mahony, D.J., Cell culture modeling of specialized tissue: Identification of genes expressed specifically by follicle-associated epithelium of Peyer{\textquoteright}s patch by expression profiling of Caco-2/Raji co-cultures (2004) International Immunology, 16 (1), pp. 91-99. , DOI 10.1093/intimm/dxh011; 
Des Rieux, A., Fievez, V., Theate, I., Mast, J., Preat, V., Schneider, Y.-J., An improved in vitro model of human intestinal follicle-associated epithelium to study nanoparticle transport by M cells (2007) European Journal of Pharmaceutical Sciences, 30 (5), pp. 380-391. , DOI 10.1016/j.ejps.2006.12.006, PII S092809870700005X; 
Kerneis, S., Bogdanova, A., Kraehenbuhl, J.-P., Pringault, E., Conversion by Peyer{\textquoteright}s patch lymphocytes of human enterocytes into M cells that transport bacteria (1997) Science, 277 (5328), pp. 949-952. , DOI 10.1126/science.277.5328.949; 
Rieux, A.D., Ragnarsson, E.G.E., Gullberg, E., Preat, V., Schneider, Y.-J., Artursson, P., Transport of nanoparticles across an in vitro model of the human intestinal follicle associated epithelium (2005) European Journal of Pharmaceutical Sciences, 25 (4-5), pp. 455-465. , DOI 10.1016/j.ejps.2005.04.015, PII S0928098705001314; 
Kadiyala, I., Loo, Y., Roy, K., Transport of chitosan-DNA nanoparticles in human intestinal M-cell model versus normal intestinal enterocytes (2010) Eur. J. Pharm. Sci., 39, pp. 103-109; 
Bazes, A., Nollevaux, G., Coco, R., Development of a triculture based system for improved benefit/risk assessment in pharmacology and human food (2011) BMC Proc., 5, pp. P67; 
Antunes, F., Andrade, F., Ferreira, D., Sarmento, B., Comparison of Two Triple Co-Culture in Vitro Cell Models to Predict Intestinal Absorption of Peptidic Drugs, , submitted; 
Antunes, F., Andrade, F., Ferreira, D., Sarmento, B., (2010) Comparison of Two Triple Co-Culture in Vitro Cell Models to Predict Intestinal Absorption of Peptidic Drugs, , PSWC2010; New Orleans;; 
Lindmark, T., Schipper, N., Lazorova, L., De Boer, A.G., Artursson, P., Absorption enhancement in intestinal epithelial Caco-2 monolayers by sodium caprate: Assessment of molecular weight dependence and demonstration of transport routes (1998) Journal of Drug Targeting, 5 (3), pp. 215-223; 
Dash, A.K., Gong, Z., Miller, D.W., Huai-Yan, H., Laforet, J.-P., Development of a rectal nicotine delivery system for the treatment of ulcerative colitis (1999) International Journal of Pharmaceutics, 190 (1), pp. 21-34. , DOI 10.1016/S0378-5173(99)00221-5, PII S0378517399002215; 
Lohikangas, L., Wilen, M., Einarsson, M., Artursson, P., Effects of a new lipid-based drug delivery system on the absorption of low molecular weight heparin (Fragmin) through monolayers of human intestinal epithelial Caco-2 cells and after rectal administration to rabbits (1994) European Journal of Pharmaceutical Sciences, 1 (6), pp. 297-305. , DOI 10.1016/0928-0987(94)90038-8; 
Lohikangas, L., Wilen, M., Einarsson, M., Artursson, P., Relative contribution of phosphatidylcholine and monoglyceride to absorption enhancement of low molecular weight heparin (Fragmin) by a new lipid-based drug delivery system in monolayers of human intestinal epithelial Caco-2 cells and after rectal administration to rabbits (1994) European Journal of Pharmaceutical Sciences, 1 (6), pp. 307-312. , DOI 10.1016/0928-0987(94)90039-6; 
Rubas, W., Cromwell, M.E.M., Shahrokh, Z., Villagran, J., Nguyen, T.-N., Wellton, M., Nguyen, T.-H., Mrsny, R.J., Flux measurements across Caco-2 monolayers may predict transport in human large intestinal tissue (1996) Journal of Pharmaceutical Sciences, 85 (2), pp. 165-169. , DOI 10.1021/js950267+; 
Gorodeski, G.I., The cultured human cervical epithelium: A new model for studying paracellular transport (1996) Journal of the Society for Gynecologic Investigation, 3 (5), pp. 267-280. , DOI 10.1016/S1071-5576(96)00028-7; 
Gorodeski, G.I., Romero, M.F., Hopfer, U., Rorke, E., Utian, W.H., Eckert, R.L., Human uterine cervical epithelial cells grown on permeable support a new model for the study of differentiation (1994) Differentiation, 56 (1-2), pp. 107-118; 
Gorodeski, G.I., Eckert, R.L., Utian, W.H., Cultured human ectocervical epithelial cell differentiation is regulated by the combined direct actions of sex steroids, glucocorticoids, and retinoids (1990) J. Clin. Endocrinol. Metab., 70, pp. 1624-1630; 
Gorodeski, G.I., Eckert, R.L., Utian, W.H., Rorke, E.A., Maintenance of in vivo-like keratin expression, sex steroid responsiveness, and estrogen receptor expression in cultured human ectocervical epithelial cells (1990) Endocrinology, 126 (1), pp. 399-406; 
Gorodeski, G.I., Merlin, D., De Santis, B.J., Frieden, K.A., Hopfer, U., Eckert, R.L., Utian, W.H., Romero, M.F., Characterization of paracellular permeability in cultured human cervical epithelium: Regulation by extracellular adenosine triphosphate (1994) Journal of the Society for Gynecologic Investigation, 1 (3), pp. 225-233; 
Gorodeski, G.I., Estrogen increases the permeability of the cultured human cervical epithelium by modulating cell deformability (1998) Am. J. Physiol., 275, pp. C888-C99; 
Gorodeski, G.I., Goldfarb, J., Seminal fluid factor increases the resistance of the tight junctional complex of cultured human cervical epithelium CaSki cells (1998) Fertility and Sterility, 69 (2), pp. 309-317. , DOI 10.1016/S0015-0282(97)00471-8, PII S0015028297004718; 
Gorodeski, G.I., Whittembury, J., A novel fluorescence chamber for the determination of volume changes in human caski cell cultures attached on filters (1998) Cell Biochemistry and Biophysics, 29 (3), pp. 307-331; 
Gorodeski, G.I., Vaginal-cervical epithelial permeability decreases after menopause (2001) Fertility and Sterility, 76 (4), pp. 753-761. , DOI 10.1016/S0015-0282(01)02377-9, PII S0015028201023779; 
Gorodeski, G.I., Aging and estrogen effects on transcervical-transvaginal epithelial permeability (2005) Journal of Clinical Endocrinology and Metabolism, 90 (1), pp. 345-351. , DOI 10.1210/jc.2004-1223; 
Gorodeski, G.I., Estrogen modulation of epithelial permeability in cervical-vaginal cells of premenopausal and postmenopausal women (2007) Menopause, 14 (6), pp. 1012-1019. , DOI 10.1097/gme.0b013e3180587eb5, PII 0004219220071406000012; 
Gorodeski, G.I., Hopfer, U., Regulation of the paracellular permeability of cultured human cervical epithelium by anucleotide receptor (1995) J. Soc. Gynecol. Investig, 2, pp. 716-720; 
Gorodeski, G.I., Jin, W., Hopfer, U., Extracellular Ca2+ directly regulates tight junctional permeability in the human cervical cell line CaSki (1997) Am. J. Physiol., 272, pp. C511-C524; 
Kaushal, G., Trombetta, L., Ochs, R.S., Shao, J., Delivery of TEM beta-lactamase by gene-transformed lactococcus lactis subsp lactis through cervical cell monolayer (2006) Int. J. Pharm., 313, pp. 29-35; 
Ayehunie, S., Cannon, C., Lamore, S., Kubilus, J., Anderson, D.J., Pudney, J., Klausner, M., Organotypic human vaginal-ectocervical tissue model for irritation studies of spermicides, microbicides, and feminine-care products (2006) Toxicology in Vitro, 20 (5), pp. 689-698. , DOI 10.1016/j.tiv.2005.10.002, PII S0887233305002171; 
Ayehunie, S., Cannon, C., Larosa, K., Development of an in vitro alternative assay method for vaginal irritation (2011) Toxicology, 279, pp. 130-138; 
Abdel-Motal, U.M., Sarkis, P.T., Han, T., Anti-gp120 minibody gene transfer to female genital epithelial cells protects against HIV-1 virus challenge in vitro (2011) PLoS One, 6, pp. e26473; 
Lagenaur, L.A., Sanders-Beer, B.E., Brichacek, B., Prevention of vaginal SHIV transmission in macaques by a live recombinant lactobacillus (2011) Mucosal. Immunol., 4, pp. 648-657; 
Fatakdawala, H., Uhland, S.A., Hydrogen peroxide mediated transvaginal drug delivery (2011) Int. J. Pharm., 409, pp. 121-127; 
Clark, M.R., McCormick, T.J., Doncel, G.F., Friend, D.R., Preclinical evaluation of UC781 microbicide vaginal drug delivery (2011) Drug. Deliv. Transl. Res., 1, pp. 175-182; 
Forbes, B., Shah, A., Martin, G.P., Lansley, A.B., The human bronchial epithelial cell line 16HBE14o- as a model system of the airways for studying drug transport (2003) International Journal of Pharmaceutics, 257 (1-2), pp. 161-167. , DOI 10.1016/S0378-5173(03)00129-7; 
Fuchs, S., Hollins, A.J., Laue, M., Schaefer, U.F., Roemer, K., Gumbleton, M., Lehr, C.-M., Differentiation of human alveolar epithelial cells in primary culture: Morphological characterization and synthesis of caveolin-1 and surfactant protein-C (2003) Cell and Tissue Research, 311 (1), pp. 31-45. , DOI 10.1007/s00441-002-0653-5; 
Forbes, B., Ehrhardt, C., Human respiratory epithelial cell culture for drug delivery applications (2005) European Journal of Pharmaceutics and Biopharmaceutics, 60 (2), pp. 193-205. , DOI 10.1016/j.ejpb.2005.02.010, PII S0939641105000731; 
Dimova, S., Brewster, M.E., Noppe, M., Jorissen, M., Augustijns, P., The use of human nasal in vitro cell systems during drug discovery and development (2005) Toxicology in Vitro, 19 (1), pp. 107-122. , DOI 10.1016/j.tiv.2004.07.003, PII S0887233304001237; 
Ehrhardt, C., Kneuer, C., Laue, M., Schaefer, U.F., Kim, K.-J., Lehr, C.-M., 16HBE14o- human bronchial epithelial cell layers express P-glycoprotein, lung resistance-related protein, and caveolin-1 (2003) Pharmaceutical Research, 20 (4), pp. 545-551. , DOI 10.1023/A:1023230328687; 
Zhu, Y., Chidekel, A., Shaffer, T.H., Cultured human airway epithelial cells (calu-3): A model of human respiratory function, structure, and inflammatory responses (2010) Crit. Care Res. Pract. 2010, , Article ID 394578, 8 pages; 
Stentebjerg-Andersen, A., Notlevsen, I.V., Brodin, B., Nielsen, C.U., Calu-3 cells grown under AIC and LCC conditions: Implications for dipeptide uptake and transepithelial transport of substances (2011) Eur. J. Pharm. Biopharm., 78, pp. 19-26; 
Grainger, C.I., Greenwell, L.L., Lockley, D.J., Martin, G.P., Forbes, B., Culture of Calu-3 cells at the air interface provides a representative model of the airway epithelial barrier (2006) Pharmaceutical Research, 23 (7), pp. 1482-1490. , DOI 10.1007/s11095-006-0255-0; 
Manford, F., Tronde, A., Jeppsson, A.-B., Patel, N., Johansson, F., Forbes, B., Drug permeability in 16HBE14o- airway cell layers correlates with absorption from the isolated perfused rat lung (2005) European Journal of Pharmaceutical Sciences, 26 (5), pp. 414-420. , DOI 10.1016/j.ejps.2005.07.010, PII S0928098705002290; 
Bur, M., Rothen-Rutishauser, B., Huwer, H., Lehr, C.M., A novel cell compatible impingement system to study in vitro drug absorption from dry powder aerosol formulations (2009) Eur. J. Pharm. Biopharm., 72, pp. 350-357; 
Hein, S., Bur, M., Schaefer, U.F., Lehr, C.M., A new Pharmaceutical Aerosol Deposition Device on Cell Cultures (PADDOCC) to evaluate pulmonary drug absorption for metered dose dry powder formulations (2011) Eur. J. Pharm. Biopharm., 77, pp. 132-138; 
Wang, Z., Zhang, Q., Transport of proteins and peptides across human cultured alveolar A549 cell monolayer (2004) International Journal of Pharmaceutics, 269 (2), pp. 451-456. , DOI 10.1016/j.ijpharm.2003.09.033; 
Lehmann, A.D., Daum, N., Bur, M., An in vitro triple cell co-culture model with primary cells mimicking the human alveolar epithelial barrier (2011) Eur. J. Pharm. Biopharm., 77, pp. 398-406; 
Steimer, A., Franke, H., Haltner-Ukomado, E., Laue, M., Ehrhardt, C., Lehr, C.-M., Monolayers of porcine alveolar epithelial cells in primary culture as an in vitro model for drug absorption studies (2007) European Journal of Pharmaceutics and Biopharmaceutics, 66 (3), pp. 372-382. , DOI 10.1016/j.ejpb.2006.11.006, PII S0939641106003286; 
Rothen-Rutishauser, B.M., Kiama, S.C., Gehr, P., A three-dimensional cellular model of the human respiratory tract to study the interaction with particles (2005) American Journal of Respiratory Cell and Molecular Biology, 32 (4), pp. 281-289. , DOI 10.1165/rcmb.2004-0187OC; 
Cooney, D., Kazantseva, M., Hickey, A.J., Development of a size-dependent aerosol deposition model utilising human airway epithelial cells for evaluating aerosol drug delivery (2004) ATLA Alternatives to Laboratory Animals, 32 (6), pp. 581-590; 
Fiegel, J., Ehrhardt, C., Schaefer, U.F., Lehr, C.-M., Hanes, J., Large porous particle impingement on lung epithelial cell monolayers - Toward improved particle characterization in the lung (2003) Pharmaceutical Research, 20 (5), pp. 788-796. , DOI 10.1023/A:1023441804464; 
Hermanns, M.I., Unger, R.E., Kehe, K., Peters, K., Kirkpatrick, C.J., Lung epithelial cell lines in coculture with human pulmonary microvascular endothelial cells: Development of an alveolo-capillary barrier in vitro (2004) Laboratory Investigation, 84 (6), pp. 736-752. , DOI 10.1038/labinvest.3700081; 
Hermanns, M.I., Fuchs, S., Bock, M., Primary human coculture model of alveolo-capillary unit to study mechanisms of injury to peripheral lung (2009) Cell Tissue. Res., 336, pp. 91-105; 
Chemuturi, N.V., Hayden, P., Klausner, M., Donovan, M.D., Comparison of human tracheal/bronchial epithelial cell culture and bovine nasal respiratory explants for nasal drug transport studies (2005) Journal of Pharmaceutical Sciences, 94 (9), pp. 1976-1985. , DOI 10.1002/jps.20404; 
Agu, R., MacDonald, C., Cowley, E., Differential expression of organic cation transporters in normal and polyps human nasal epithelium: Implications for in vitro drug delivery studies (2011) Int. J. Pharm., 406, pp. 49-54; 
Agu, R.U., Vu Dang, H., Jorissen, M., Willems, T., Kinget, R., Verbeke, N., Nasal absorption enhancement strategies for therapeutic peptides: An in vitro study using cultured human nasal epithelium (2002) International Journal of Pharmaceutics, 237 (1-2), pp. 179-191. , DOI 10.1016/S0378-5173(02)00039-X, PII S037851730200039X; 
Kissel, T., Werner, U., Nasal delivery of peptides: An in vitro cell culture model for the investigation of transport and metabolism in human nasal epithelium (1998) Journal of Controlled Release, 53 (1-3), pp. 195-203. , DOI 10.1016/S0168-3659(97)00253-8, PII S0168365997002538; 
Lin, H., Yoo, J.-W., Roh, H.-J., Lee, M.-K., Chung, S.-J., Shim, C.-K., Kim, D.-D., Transport of anti-allergic drugs across the passage cultured human nasal epithelial cell monolayer (2005) European Journal of Pharmaceutical Sciences, 26 (2), pp. 203-210. , DOI 10.1016/j.ejps.2005.06.003, PII S0928098705001958; 
Kim, D.D., In vitro cellular models for nasal drug absorption studies (2008) Drug Absorption Studies, pp. 216-234. , Situ In Vitro and In Silico Models.Ehrhardt C, Kim K-J, editors. Springer; New York:; 
Wang, S., Chow, M.S., Zuo, Z., An approach for rapid development of nasal delivery of analgesics-identification of relevant features, in vitro screening and in vivo verification (2011) Int. J. Pharm., 420, pp. 43-50; 
Wengst, A., Reichl, S., RPMI 2650 epithelial model and three-dimensional reconstructed human nasal mucosa as in vitro models for nasal permeation studies (2010) Eur. J. Pharm. Biopharm., 74, pp. 290-297; 
Enriquez-De-Salamanca, A., Calder, V., Gao, J., Cytokine responses by conjunctival epithelial cells: An in vitro model of ocular inflammation (2008) Cytokine, 44, pp. 160-167; 
O{\textquoteright}Sullivan, N.L., Baylor III, A.E., Montgomery, P.C., Development of immortalized rat conjunctival epithelial cell lines: An in vitro model to examine transepithelial antigen delivery (2007) Experimental Eye Research, 84 (2), pp. 323-331. , DOI 10.1016/j.exer.2006.10.005, PII S0014483506003988; 
Kuehn, M.H., Kim, C.Y., Ostojic, J., Bellin, M., Alward, W.L.M., Stone, E.M., Sakaguchi, D.S., Kwon, Y.H., Retinal synthesis and deposition of complement components induced by ocular hypertension (2006) Experimental Eye Research, 83 (3), pp. 620-628. , DOI 10.1016/j.exer.2006.03.002, PII S0014483506001850; 
Hornof, M., Toropainen, E., Urtti, A., Cell culture models of the ocular barriers (2005) European Journal of Pharmaceutics and Biopharmaceutics, 60 (2), pp. 207-225. , DOI 10.1016/j.ejpb.2005.01.009, PII S0939641105000214; 
Silva, S., Costa, J., Pintado, M., Antioxidants in the prevention and treatment of diabetic retinopathy - A review (2010) J. Diabetes. Metab., 1, pp. 111-120; 
Fuente, M.D.L., Ravina, M., Paolicelli, P., Chitosan-based nanostructures: A delivery platform for ocular therapeutics (2010) Adv. Drug. Deliv. Rev., 62, pp. 100-117; 
Gukasyan, H.J., Kim, K.-J., Lee, V.H.L., The conjunctival barrier in ocular drug delivery (2008) Drug Absorption Studies, pp. 307-320. , Ehrhardt C, Kim KJ, editors. In Situ, In Vitro and in Silico. Springer; New York:; 
Toropainen, E., Ranta, V.-P., Talvitte, A., Suhonen, P., Urtti, A., Culture model of human corneal epithelium for prediction of ocular drug absorption (2001) Investigative Ophthalmology and Visual Science, 42 (12), pp. 2942-2948; 
Gunda, S., Hariharan, S., Mandava, N., Mitra, A.K., Barriers in ocular drug delivery (2008) Ophthalmology Research: Ocular Transporters in Ophthalmic Diseases and Drug Delivery, pp. 399-413. , Barnstable JT-TaCJ, editor. Human Press; Hershey:; 
Kudela, P., Koller, V.J., Mayr, U.B., Bacterial ghosts as antigen and drug delivery system for ocular surface diseases: Effective internalization of bacterial ghosts by human conjunctival epithelial cells (2011) J. Biotechnol., 153, pp. 167-175; 
Barar, J., Asadi, M., Abdolreza, S.A., Mortazavi-Tabatabaei Omidi, Y., Ocular drug delivery; Impact of in vitro cell culture models (2009) J. Ophthalmol. Vision. Res., 4, pp. 238-252; 
Aukunuru, J.V., Sunkara, G., Ayalasomayajula, S.P., Deruiter, J., Clark, R.C., Kompella, U.B., A biodegradable injectable implant sustains systemic and ocular delivery of an aldose reductase inhibitor and ameliorates biochemical changes in a galactose-fed rat model for diabetic complications (2002) Pharmaceutical Research, 19 (3), pp. 278-285. , DOI 10.1023/A:1014438800893; 
Luthra, S., Narayanan, R., Marques, L.E.A., Chwa, M., Kim, D.W., Dong, J., Seigel, G.M., Kuppermann, B.D., Evaluation of in vitro effects of bevacizumab (Avastin) on retinal pigment epithelial, neurosensory retinal, and microvascular endothelial cells (2006) Retina, 26 (5), pp. 512-518. , DOI 10.1097/01.iae.0000222547.35820.52, PII 0000698220060500000003; 
Flamand, N., Marrot, L., Belaidi, J.-P., Bourouf, L., Dourille, E., Feltes, M., Meunier, J.-R., Development of genotoxicity test procedures with Episkin, a reconstructed human skin model: Towards new tools for in vitro risk assessment of dermally applied compounds? (2006) Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 606 (1-2), pp. 39-51. , DOI 10.1016/j.mrgentox.2006.02.009, PII S1383571806000593; 
Schaefer, U.F., Hansen, S., Schneider, M., Models for skin absorption and skin toxicity testing (2008) Drug Absorption Studies, pp. 3-33. , Ehrhardt C, Kim K-J, editors. Springer; US:; 
Godin, B., Touitou, E., Transdermal skin delivery: Predictions for humans from in vivo, ex vivo and animal models (2007) Advanced Drug Delivery Reviews, 59 (11), pp. 1152-1161. , DOI 10.1016/j.addr.2007.07.004, PII S0169409X07001718, Prediction of Therapeutic and Drug Delivery Outcomes Using Animal Models; 
Netzlaff, F., Lehr, C.-M., Wertz, P.W., Schaefer, U.F., The human epidermis models EpiSkin, SkinEthic and EpiDerm An evaluation of morphology and their suitability for testing phototoxicity, irritancy, corrosivity, and substance transport (2005) European Journal of Pharmaceutics and Biopharmaceutics, 60 (2), pp. 167-178. , DOI 10.1016/j.ejpb.2005.03.004, PII S0939641105001116; 
Hu, T., Bailey, R.E., Morrall, S.W., Dermal penetration and metabolism of p-aminophenol and p-phenylenediamine: Application of the EpiDerm human reconstructed epidermis model (2009) Toxicol. Lett., 188, pp. 119-129; 
Gabbanini, S., Matera, R., Beltramini, C., Analysis of in vitro release through reconstructed human epidermis and synthetic membranes of multi-vitamins from cosmetic formulations (2010) J. Pharm. Biomed. Anal., 52, pp. 461-467; 
Balimane, P.V., Chong, S., Cell culture-based models for intestinal permeability: A critique (2005) Drug Discovery Today, 10 (5), pp. 335-343. , DOI 10.1016/S1359-6446(04)03354-9, PII S1359644604033549
},
	month = {2012///},
	pages = {607 - 621},
	abstract = {In vitro cell models have been used to predict drug permeation in early stages of drug development, since they represent an easy and reproducible method, allowing the tracking of drug absorption rate and mechanism, with an advantageous costbenefit ratio. Such cell-based models are mainly composed of immortalized cells with an intrinsic ability to grow in a monolayer when seeded in permeable supports, maintaining their physiologic characteristics regarding epithelium cell physiology and functionality. Areas covered: This review summarizes the most important intestinal, pulmonary, nasal, vaginal, rectal, ocular and skin cell-based in vitro models for predicting the permeability of drugs. Moreover, the similitude between in vitro cell models and in vivo conditions are discussed, providing evidence that each model may provisionally resemble different drug absorption route. Expert opinion: Despite the widespread use of in vitro cell models for drug permeability and absorption evaluation purposes, a detailed study on the properties of these models and their in vitroin vivo correlation compared with human data are required to further use in order to consider a future drug discovery optimization and clinical development. {\textcopyright} 2012 Informa UK, Ltd.},
	keywords = {Animals, Caco-2 Cells, carcinoma cell, cell differentiation, cell growth, Cell Line, Cell Membrane Permeability, cell models, cell proliferation, cell strain CACO 2, cell type, Cells, conjunctiva, drug absorption, drug bioavailability, drug delivery system, drug penetration, drug screening, drug transport, epithelium cell, Eye, human, Humans, in vitro models, in vitro study, in vitroin vivo correlation, Intestinal Absorption, intestinal mucosa, intestine cell, Intestines, Lung, lung alveolus cell, Models, Biological, molecular weight, nasal cell, nonhuman, ocular cell, permeability, Pharmaceutical Preparations, rectal cell, review, Skin, skin cell, vaginal cell},
	isbn = {17425255 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84859967380\&partnerID=40\&md5=f531a0a6e96c96e8daf71a6b36dbec8a},
	author = {Sarmento, B. and Andrade, F. and Da Silva, S.B. and Rodrigues, F. and Das Neves, J. and Ferreira, D.}
}
@article {2180,
	title = {Chitosan drives anti-inflammatory macrophage polarisation and pro-inflammatory dendritic cell stimulation.},
	journal = {European cells \& materialsEur Cell Mater},
	volume = {24},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusPubMed ID: 22828991Language of Original Document: EnglishCorrespondence Address: Oliveira, M.I.Chemicals/CAS: chitosan, 9012-76-4; Antigens, CD86; Biocompatible Materials; Chitosan, 9012-76-4; Histocompatibility Antigens Class II; IL10 protein, human; Interleukin-10, 130068-27-8; Transforming Growth Factor beta1; Tumor Necrosis Factor-alpha},
	month = {2012///},
	pages = {136 - 152; discussion 152-153},
	abstract = {Macrophages and dendritic cells (DC) share the same precursor and play key roles in immunity. Modulation of their behaviour to achieve an optimal host response towards an implanted device is still a challenge. Here we compare the differentiation process and polarisation of these related cell populations and show that they exhibit different responses to chitosan (Ch), with human monocyte-derived macrophages polarising towards an anti-inflammatory phenotype while their DC counterparts display pro-inflammatory features. Macrophages and DC, whose interactions with biomaterials are frequently analysed using fully differentiated cells, were cultured directly on Ch films, rather than exposed to the polymer after complete differentiation. Ch was the sole stimulating factor and activated both macrophages and DC, without leading to significant T cell proliferation. After 10 d on Ch, macrophages significantly down-regulated expression of pro-inflammatory markers, CD86 and MHCII. Production of pro-inflammatory cytokines, particularly TNF-α, decreased with time for cells cultured on Ch, while anti-inflammatory IL-10 and TGF-β1, significantly increased. Altogether, these results suggest an M2c polarisation. Also, macrophage matrix metalloproteinase activity was augmented and cell motility was stimulated by Ch. Conversely, DC significantly enhanced CD86 expression, reduced IL-10 secretion and increased TNF-α and IL-1β levels. Our findings indicate that cells with a common precursor may display different responses, when challenged by the same biomaterial. Moreover, they help to further comprehend macrophage/DC interactions with Ch and the balance between pro- and anti-inflammatory signals associated with implant biomaterials. We propose that an overall pro-inflammatory reaction may hide the expression of anti-inflammatory cytokines, likely relevant for tissue repair/regeneration.},
	keywords = {antibody specificity, Antigens, CD86, article, Biocompatible Materials, biomaterial, biosynthesis, CD86 antigen, Cell culture, cell differentiation, cell motion, Cell Movement, Cells, Cultured, chitosan, cytology, dendritic cell, Dendritic Cells, drug effect, flow cytometry, Histocompatibility Antigens Class II, HLA antigen class 2, human, Humans, IL10 protein, human, immunology, interleukin 10, Interleukin-10, macrophage, Macrophages, metabolism, monocyte, Monocytes, Organ Specificity, transforming growth factor beta1, tumor necrosis factor alpha, Tumor Necrosis Factor-alpha},
	isbn = {14732262 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84868253478\&partnerID=40\&md5=5a496411709ccbe5d3d70de13b58b08a},
	author = {Oliveira, M.I. and Santos, S.G. and Oliveira, M.J. and Torres, A.L. and Barbosa, M.A.}
}
@book {2221,
	title = {Classification-based segmentation of the region of interest in chromatographic images},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {6754 LNCS},
	year = {2011},
	note = {Conference code: 85546Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-21596-4_8Language of Original Document: EnglishCorrespondence Address: Sousa, A.V.; Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua Roberto Frias, s/n, 4200-465 Porto, Portugal; email: asousa@eu.ipp.ptReferences: Zarate, Y., Hopkin, R., Fabry{\textquoteright}s Disease (2008) The Lancet, 372 (9647), pp. 1427-1435;Linthorst, G.E., Vedder, A., Aerts, J.M., Hollak, C.E., Screening for Fabry Disease Using Whole Blood Spots Fails to Identify One-third of Female Carriers (2005) Clinica Chimica Acta, 353 (1-2), pp. 201-203; 
Eng, C.M., Germain, D.P., Banikazemi, M., Warnock, D.G., Wanner, C., Hopkin, R.J., Bultas, J., Wilcox, W.R., Fabry Disease: Guidelines for the Evaluation and Management of Multi-organ System Involvement (2006) Genetics in Medicine, 8 (9), pp. 539-548; 
Gerasimov, A.V., Use of the Software Processing of Scanned Chromatogram Images in Quantitative Planar Chromatography (2004) J. of Anal. Chem., 59 (4), pp. 348-353; 
Bajla, I., Hollander, I., Fluch, S., Burg, K., Kollar, M., An Alternative Method for Electrophoretic Gel Image Analysis in the Gelmaster Software (2005) Comput. Methods Programs Biomed., 77 (3), pp. 209-231; 
Sousa, A.V., Mendon{\c c}a, A.M., Campilho, A., Chromatographic Pattern Classification (2008) IEEE Trans. on Biomedical Engineering, 55 (6), pp. 1687-1696; 
Bajla, I., Rubl{\'\i}k, F., Arendack{\'a}, B., Farka{\v s}, I., Horni{\v s}ov{\'a}, K., {\v S}tolc, S., Witkovsk{\'y}, V., Segmentation and Supervised Classification of Image Objects in Epo Doping-control (2009) Machine Vision and Applications, 20 (4), pp. 243-259; 
Sousa, A.V., Aguiar, R., Mendon{\c c}a, A.M., Campilho, A., Automatic Lane and Band Detection in Images of Thin Layer Chromatography (2004) LNCS, 3212, pp. 158-165. , Campilho, A.C., Kamel, M.S. (eds.) ICIAR 2004. Springer, Heidelberg; 
Mendon{\c c}a, A.M., Sousa, A.V., S{\'a}-Miranda, M.C., Campilho, A., Automatic segmentation of chromatographic images for region of interest delineation (2011) SPIE Medical Imaging; 
Dempster, A., Laird, N., Rubin, D., Maximum Likelihood from Incomplete Data via the EM Algorithm (1997) J. of the Royal Statistical Society. Series B, 39 (1), pp. 1-38; 
Heijden, F., Robert, P.W.D., Ridder, D., Tax, D.M.J., (2004) Classification, Parameter Estimation and State Estimation, , John Wiley \& Sons, Chichester
},
	month = {2011///},
	pages = {68 - 78},
	address = {Burnaby, BC},
	abstract = {This paper proposes a classification-based method for automating the segmentation of the region of interest (ROI) in digital images of chromatographic plates. Image segmentation is performed in two phases. In the first phase an unsupervised learning method classifies the image pixels into three classes: frame, ROI or unknown. In the second phase, distance features calculated for the members of the three classes are used for deciding on the new label, ROI or frame, for each individual connected segment previously classified as unknown.The segmentation result is post-processed using a sequence of morphological operators before obtaining the final ROI rectangular area. The proposed methodology, which is the initial step for the development of a screening tool for Fabry disease, was successfully evaluated in a dataset of 58 chromatographic images. {\textcopyright} 2011 Springer-Verlag.},
	keywords = {Chromatographic images, Chromatographic plates, Chromatography, Data sets, Digital image, Distance feature, Fabry disease, Image analysis, Image pixels, Image segmentation, Morphological operator, Rectangular area, Region of interest, Region of interest delineation, Screening tool, Second phase, Segmentation results, Unsupervised learning, Unsupervised learning method},
	isbn = {03029743 (ISSN); 9783642215957 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79960331409\&partnerID=40\&md5=c248f56c8a7bda71832c4b569e32164b},
	author = {Sousa, A.V. and Mendon{\c c}a, A.M. and S{\'a}-Miranda, M.C. and Campilho, A.}
}
@article {2230,
	title = {Complexity and categorical analysis may improve the interpretation of agreement studies using continuous variables},
	journal = {Journal of Evaluation in Clinical PracticeJ. Eval. Clin. Pract.},
	volume = {17},
	year = {2011},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: JECPFdoi: 10.1111/j.1365-2753.2011.01668.xPubMed ID: 21489051Language of Original Document: EnglishCorrespondence Address: Costa-Santos, C.; Department of Biostatistics and Medical Informatics, Faculty of Medicine, University of Porto, Al. Prof. Hern{\^a}ni Monteiro, 4200-319 Porto, Portugal; email: csantos@med.up.ptReferences: Costa Santos, C., Costa Pereira, A., Bernardes, J., Agreement studies in obstetrics and gynaecology: Inappropriateness, controversies and consequences (2005) BJOG: An International Journal of Obstetrics and Gynaecology, 112 (5), pp. 667-669. , DOI 10.1111/j.1471-0528.2004.00505.x;Khan, K.S., Chien, P.F., Evaluation of a clinical test. I: Assessment of reliability (2001) BJOG, 108, pp. 562-567; 
Luiz, R.R., Szklo, M., More than one statistical strategy to assess agreement of quantitative measurements may usefully be reported (2005) Journal of Clinical Epidemiology, 58 (3), pp. 215-216. , DOI 10.1016/j.jclinepi.2004.07.007; 
Costa-Santos, C., Bernardes, J., Ayres-De-Campos, D., Costa, A., Costa, C., The limits of agreement and the intraclass correlation coefficient may be inconsistent in the interpretation of agreement (2011) Journal of Clinical Epidemiology, 64 (3), pp. 264-269; 
Costa-Santos, C., Antunes, L., Souto, A., Bernardes, J., Assessment of disagreement: A new information-based approach (2010) Annals of Epidemiology, 20, pp. 555-561; 
Luiz, R.R., Costa, A.J.L., Kale, P.L., Werneck, G.L., Assessment of agreement of a quantitative variable: A new graphical approach (2003) Journal of Clinical Epidemiology, 56 (10), pp. 963-967. , DOI 10.1016/S0895-4356(03)00164-1; 
Plsek, P.E., Greenhalgh, T., The challenge of complexity in health care (2001) British Medical Journal, 323 (7313), pp. 625-628; 
Shiell, A., Hawe, P., Gold, L., Complex interventions or complex systems? Implications for health economic evaluation (2008) BMJ, 336 (7656), pp. 1281-1283; 
Wilson, T., Holt, T., Complexity science: Complexity and clinical care (2001) British Medical Journal, 323 (7314), pp. 685-688; 
Miles, A., Complexity in medicine and healthcare: People and systems, theory and practice (2009) Journal of Evaluation in Clinical Practice, 15 (3), pp. 409-410; 
Shannon, {\^I}., A mathematical theory of communication (1948) Bell System Technical Journal, 27, pp. 379-423. , 623- 656; 
Bernardes, J., Costa-Pereira, A., Ayres-De-Campos, D., Van Geijn, H.P., Pereira-Leite, L., Evaluation of interobserver agreement of cardiotocograms (1997) International Journal of Gynecology and Obstetrics, 57 (1), pp. 33-37. , DOI 10.1016/S0020-7292(97)02846-4, PII S0020729297028464; 
Ayres-De-Campos, D., Bernardes, J., Costa-Pereira, A., Pereira-Leite, L., Inconsistencies in classification by experts of cardiotocograms and subsequent clinical decision (1999) British Journal of Obstetrics and Gynaecology, 106, pp. 1307-1310; 
Rooth, G., Guidelines for the use of fetal monitoring (1987) International Journal of Gynaecology and Obstetrics, 25, pp. 159-167; 
Grant, J.M., The fetal heart rate is normal, isn{\textquoteright}t it? Observer agreement of categorical assessments (1991) Lancet, 337, pp. 215-218; 
M{\"u}ller, R., B{\"u}ttner, P., A critical discussion of intraclass correlation coefficients (1994) Statistics in Medicine, 13, pp. 2465-2476; 
Byrt, T., Bishop, J., Carlin, J.B., Bias, prevalence and kappa (1993) Journal of Clinical Epidemiology, 46 (5), pp. 423-429. , DOI 10.1016/0895-4356(93)90018-V; 
West, B.J., Homeostasis and Gauss statistics: Barriers to understanding natural variability (2010) Journal of Evaluation in Clinical Practice, 16, pp. 403-408; 
Goncalves, H., Rocha, A.P., Ayres-de-Campos, D., Bernardes, J., Linear and nonlinear fetal heart rate analysis of normal and acidemic fetuses in the minutes preceding delivery (2006) Medical and Biological Engineering and Computing, 44 (10), pp. 847-855. , DOI 10.1007/s11517-006-0105-6; 
Costa Santos, C., Vitanyi, P.M.B., Bernardes, J., Antunes, L., Clustering fetal heart rate tracings by compression (2006) Proceedings - IEEE Symposium on Computer-Based Medical Systems, 2006, pp. 685-690. , DOI 10.1109/CBMS.2006.68, 1647650, Proceedings - 19th IEEE International Symposium on Computer-Based Medical Systems, CBMS 2006
},
	month = {2011///},
	pages = {511 - 514},
	abstract = {Rationale Complex clinical scenarios involving a high degree of uncertainty frequently lead to a poor agreement over diagnosis and management. However, inconsistent results can be found with the most widely used measures of agreement for continuous variables - the limits of agreement and the intraclass correlation coefficient. Aims and objectives We aim to improve the interpretation of agreement studies using continues variables. Methods and results Evaluation of agreement may be improved by complexity analysis and by categorization of variables, followed by the use of the proportions of agreement. Conclusions The average never characterizes a complex phenomenon and the methods used to access agreement in continuous variables are based on the mean. For future agreement studies, involving complex continuous variables, we recommend a complexity and categorical analysis. {\textcopyright} 2011 Blackwell Publishing Ltd.},
	keywords = {article, Biomedical research, Cardiotocography, clinical decision making, clinical practice, complexity, Data Interpretation, Statistical, Decision Making, entropy, Female, fetus heart rate, Heart Rate, Fetal, human, Humans, observer variation, Pregnancy, Pregnancy Outcome, priority journal, Reproducibility of Results, statistical data interpretation, Uncertainty},
	isbn = {13561294 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79956081274\&partnerID=40\&md5=bd7376dca48228f4c4b1b21389689596},
	author = {Costa-Santos, C. and Bernardes, J. and Antunes, L. and Ayres-de-Campos, D.}
}
@article {2236,
	title = {Covalent immobilization of antimicrobial peptides (AMPs) onto biomaterial surfaces},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {7},
	year = {2011},
	note = {Cited By (since 1996): 24Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2010.11.005PubMed ID: 21056701Language of Original Document: EnglishCorrespondence Address: Martins, M. C. L.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade Do Porto, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: cmartins@ineb.up.ptChemicals/CAS: amoxicillin, 26787-78-0, 34642-77-8, 61336-70-7; ampicillin, 69-52-3, 69-53-4, 7177-48-2, 74083-13-9, 94586-58-0; carbenicillin, 17230-86-3, 4697-36-3, 4800-94-6; cefazolin, 25953-19-9, 27164-46-1; gentamicin, 1392-48-9, 1403-66-3, 1405-41-0; penicillin G, 1406-05-9, 61-33-6; teicoplanin, 61036-62-2, 61036-64-4; tobramycin, 32986-56-4; vancomycin, 1404-90-6, 1404-93-9; Antimicrobial Cationic Peptides; Biocompatible Materials; Coated Materials, Biocompatible; Immobilized ProteinsReferences: Glinel, K., Jonas, A.M., Jouenne, T., Leprince, J., Galas, L., Huck, W.T., Antibacterial and antifouling polymer brushes incorporating antimicrobial peptide (2009) Bioconj Chem, 20 (1), pp. 71-77;Vila, J., Soriano, A., Mensa, J., Molecular basis of microbial adherence to prosthetic materials. Role of biofilms in prosthesis-associated infection (2008) Enferm Infecc Microbiol Clin, 26 (1), pp. 48-54; 
Hetrick, E.M., Schoenfisch, M.H., Reducing implant-related infections: Active release strategies (2006) Chemical Society Reviews, 35 (9), pp. 780-789. , DOI 10.1039/b515219b; 
Darouiche, R.O., Treatment of Infections Associated with Surgical Implants (2004) New England Journal of Medicine, 350 (14), pp. 1422-1429. , DOI 10.1056/NEJMra035415; 
Zhao, L., Chu, P.K., Zhang, Y., Wu, Z., Antibacterial coatings on titanium implants (2009) J Biomed Mater Res B Appl Biomater, 91 (1), pp. 470-480; 
Dee, K.C., Puleo, D.A., Bizios, R., (2002) An Introduction to Tissue-biomaterial Interactions, , Wiley-Liss New York; 
Subramani, K., Jung, R.E., Molenberg, A., Hammerle, C.H., Biofilm on dental implants: A review of the literature (2009) Int J Oral Maxillofac Implants, 24 (4), pp. 616-626; 
Fey, P.D., Olson, M.E., Current concepts in biofilm formation of Staphylococcus epidermidis (2010) Future Microbiol, 5 (6), pp. 917-933; 
De Carvalho, C.C., Biofilms: Recent developments on an old battle (2007) Recent Pat Biotechnol, 1 (1), pp. 49-57; 
Costerton, J.W., Stewart, P.S., Greenberg, E.P., Bacterial biofilms: A common cause of persistent infections (1999) Science, 284 (5418), pp. 1318-1322. , DOI 10.1126/science.284.5418.1318; 
Willcox, M.D., Hume, E.B., Aliwarga, Y., Kumar, N., Cole, N., A novel cationic-peptide coating for the prevention of microbial colonization on contact lenses (2008) J Appl Microbiol, 105 (6), pp. 1817-1825; 
Lewis, K., Riddle of biofilm resistance (2001) Antimicrobial Agents and Chemotherapy, 45 (4), pp. 999-1007. , DOI 10.1128/AAC.45.4.999-1007.2001; 
Monteiro, D.R., Gorup, L.F., Takamiya, A.S., Ruvollo-Filho, A.C., De Camargo, E.R., Barbosa, D.B., The growing importance of materials that prevent microbial adhesion: Antimicrobial effect of medical devices containing silver (2009) Int J Antimicrob Agents, 34 (2), pp. 103-110; 
Gooderham, W.J., Bains, M., McPhee, J.B., Wiegand, I., Hancock, R.E., Induction by cationic antimicrobial peptides and involvement in intrinsic polymyxin and antimicrobial peptide resistance, biofilm formation, and swarming motility of PsrA in Pseudomonas aeruginosa (2008) J Bacteriol, 190 (16), pp. 5624-5634; 
Bagge, N., Schuster, M., Hentzer, M., Ciofu, O., Givskov, M., Greenberg, E.P., Hoiby, N., Pseudomonas aeruginosa Biofilms Exposed to Imipenem Exhibit Changes in Global Gene Expression and β-Lactamase and Alginate Production (2004) Antimicrobial Agents and Chemotherapy, 48 (4), pp. 1175-1187. , DOI 10.1128/AAC.48.4.1175-1187.2004; 
Ahmed, N.A., Petersen, F.C., Scheie, A.A., AI-2/LuxS is involved in increased biofilm formation by Streptococcus intermedius in the presence of antibiotics (2009) Antimicrob Agents Chemother, 53 (10), pp. 4258-4263; 
Hoffman, L.R., D{\textquoteright}Argenio, D.A., MacCoss, M.J., Zhang, Z., Jones, R.A., Miller, S.I., Aminoglycoside antibiotics induce bacterial biofilm formation (2005) Nature, 436 (7054), pp. 1171-1175. , DOI 10.1038/nature03912; 
Harbers, G.M., Emoto, K., Greef, C., Metzger, S.W., Woodward, H.N., Mascali, J.J., Grainger, D.W., Lochhead, M.J., Functionalized poly(ethylene glycol)-based bioassay surface chemistry that facilitates bio-immobilization and inhibits nonspecific protein, bacterial, and mammalian cell adhesion (2007) Chemistry of Materials, 19 (18), pp. 4405-4414. , DOI 10.1021/cm070509u; 
Chapman, R.G., Ostuni, E., Liang, M.N., Meluleni, G., Kim, E., Yan, L., Pier, G., Whitesides, G.M., Polymeric thin films that resist the adsorption of proteins and the adhesion of bacteria (2001) Langmuir, 17 (4), pp. 1225-1233. , DOI 10.1021/la001222d; 
Goncalves, I.C., Martins, M.C., Barbosa, M.A., Naeemi, E., Ratner, B.D., Selective protein adsorption modulates platelet adhesion and activation to oligo(ethylene glycol)-terminated self-assembled monolayers with C18 ligands (2009) J Biomed Mater Res A, 89 (3), pp. 642-653; 
Chung, D., Papadakis, S.E., Yam, K.L., Evaluation of a polymer coating containing triclosan as the antimicrobial layer for packaging materials (2003) International Journal of Food Science and Technology, 38 (2), pp. 165-169. , DOI 10.1046/j.1365-2621.2003.00657.x; 
Leung, D., Spratt, D.A., Pratten, J., Gulabivala, K., Mordan, N.J., Young, A.M., Chlorhexidine-releasing methacrylate dental composite materials (2005) Biomaterials, 26 (34), pp. 7145-7153. , DOI 10.1016/j.biomaterials.2005.05.014, PII S0142961205003881; 
Ravikumar, T., Murata, H., Koepsel, R.R., Russell, A.J., Surface-active antifungal polyquaternary amine (2006) Biomacromolecules, 7 (10), pp. 2762-2769. , DOI 10.1021/bm060476w; 
Bach, A., Darby, D., Bottiger, B., Bohrer, H., Motsch, J., Martin, E., Retention of the antibiotic teicoplanin on a hydromer-coated central venous catheter to prevent bacterial colonization in postoperative surgical patients (1996) Intensive Care Medicine, 22 (10), pp. 1066-1069. , DOI 10.1007/s001340050214; 
Jose, B., Antoci Jr., V., Zeiger, A.R., Wickstrom, E., Hickok, N.J., Vancomycin covalently bonded to titanium beads kills Staphylococcus aureus (2005) Chemistry and Biology, 12 (9), pp. 1041-1048. , DOI 10.1016/j.chembiol.2005.06.013, PII S1074552105002309; 
Kilic, D., Agalar, C., Ozturk, E., Denkbas, E.B., Cime, A., Agalar, F., Antimicrobial activity of cefazolin-impregnated mesh grafts (2007) ANZ Journal of Surgery, 77 (4), pp. 256-260. , DOI 10.1111/j.1445-2197.2007.04029.x; 
Gallardo-Moreno, A.M., Pacha-Olivenza, M.A., Saldana, L., Perez-Giraldo, C., Bruque, J.M., Vilaboa, N., In vitro biocompatibility and bacterial adhesion of physico-chemically modified Ti6Al4V surface by means of UV irradiation (2009) Acta Biomater, 5 (1), pp. 181-192; 
Jansen, B., Kohnen, W., Prevention of biofilm formation by polymer modification (1995) Journal of Industrial Microbiology, 15 (4), pp. 391-396. , DOI 10.1007/BF01569996; 
Martins, M.C.L., Wang, D., Ji, J., Feng, L., Barbosa, M.A., Albumin and fibrinogen adsorption on Cibacron blue F3G-A immobilised onto PU-PHEMA (polyurethane-poly (hydroxyethylmethacrylate)) surfaces (2003) Journal of Biomaterials Science, Polymer Edition, 14 (5), pp. 439-455. , DOI 10.1163/156856203766652057; 
Zhang, F., Zhang, Z., Zhu, X., Kang, E.T., Neoh, K.G., Silk-functionalized titanium surfaces for enhancing osteoblast functions and reducing bacterial adhesion (2008) Biomaterials, 29 (36), pp. 4751-4759; 
Flemming, R.G., Capelli, C.C., Cooper, S.L., Proctor, R.A., Bacterial colonization of functionalized polyurethanes (2000) Biomaterials, 21 (3), pp. 273-281; 
Singla, A.K., Chawla, M., Chitosan: Some pharmaceutical and biological aspects - An update (2001) Journal of Pharmacy and Pharmacology, 53 (8), pp. 1047-1067. , DOI 10.1211/0022357011776441; 
Murata, H., Koepsel, R.R., Matyjaszewski, K., Russell, A.J., Permanent, non-leaching antibacterial surfaces-2: How high density cationic surfaces kill bacterial cells (2007) Biomaterials, 28 (32), pp. 4870-4879. , DOI 10.1016/j.biomaterials.2007.06.012, PII S0142961207004681; 
Aumsuwan, N., Danyus, R.C., Heinhorst, S., Urban, M.W., Attachment of ampicillin to expanded poly(tetrafluoroethylene): Surface reactions leading to inhibition of microbial growth (2008) Biomacromolecules, 9 (7), pp. 1712-1718; 
Cevher, E., Orhan, Z., Mulazimoglu, L., Sensoy, D., Alper, M., Yildiz, A., Characterization of biodegradable chitosan microspheres containing vancomycin and treatment of experimental osteomyelitis caused by methicillin-resistant Staphylococcus aureus with prepared microspheres (2006) Int J Pharm, 317 (2), pp. 127-135; 
Faber, C., Stallmann, H.P., Lyaruu, D.M., Joosten, U., Von Eiff, C., Van Nieuw Amerongen, A., Wuisman, P.I.J.M., Comparable efficacies of the antimicrobial peptide human lactoferrin 1-11 and gentamicin in a chronic methicillin-resistant Staphylococcus aureus osteomyelitis model (2005) Antimicrobial Agents and Chemotherapy, 49 (6), pp. 2438-2444. , DOI 10.1128/AAC.49.6.2438-2444.2005; 
Aumsuwan, N., Heinhorst, S., Urban, M.W., Antibacterial surfaces on expanded polytetrafluoroethylene; penicillin attachment (2007) Biomacromolecules, 8 (2), pp. 713-718. , DOI 10.1021/bm061050k; 
Palumbi, S.R., Humans as the world{\textquoteright}s greatest evolutionary force (2001) Science, 293 (5536), pp. 1786-1790. , DOI 10.1126/science.293.5536.1786; 
Andreu, D., Rivas, L., Animal antimicrobial peptides: An overview (1998) Biopolymers, 47 (6), pp. 415-433; 
Rivas, L., Luque-Ortega, J.R., Andreu, D., Amphibian antimicrobial peptides and Protozoa: Lessons from parasites (2009) Biochim Biophys Acta, 1788 (8), pp. 1570-1581; 
Gordon, Y.J., Romanowski, E.G., McDermott, A.M., Mini review: A review of antimicrobial peptides and their therapeutic potential as anti-infective drugs (2005) Current Eye Research, 30 (7), pp. 505-515. , DOI 10.1080/02713680590968637; 
Guani-Guerra, E., Santos-Mendoza, T., Lugo-Reyes, S.O., Teran, L.M., Antimicrobial peptides: General overview and clinical implications in human health and disease (2010) Clin Immunol, 135 (1), pp. 1-11; 
Perron, G.G., Zasloff, M., Bell, G., Experimental evolution of resistance to an antimicrobial peptide (2006) Proceedings of the Royal Society B: Biological Sciences, 273 (1583), pp. 251-256. , DOI 10.1098/rspb.2005.3301; 
Reddy, K.V.R., Yedery, R.D., Aranha, C., Antimicrobial peptides: Premises and promises (2004) International Journal of Antimicrobial Agents, 24 (6), pp. 536-547. , DOI 10.1016/j.ijantimicag.2004.09.005, PII S092485790400322X; 
Van {\textquoteright}T Hof, W., Veerman, E.C.I., Heimerhorst, E.J., Nieuw Amerongen, A.V., Antimicrobial peptides: Properties and applicability (2001) Biological Chemistry, 382 (4), pp. 597-619. , DOI 10.1515/BC.2001.072; 
Li, M., Cha, D.J., Lai, Y., Villaruz, A.E., Sturdevant, D.E., Otto, M., The antimicrobial peptide-sensing system aps of Staphylococcus aureus (2007) Molecular Microbiology, 66 (5), pp. 1136-1147. , DOI 10.1111/j.1365-2958.2007.05986.x; 
Li, M., Lai, Y., Villaruz, A.E., Cha, D.J., Sturdevant, D.E., Otto, M., Gram-positive three-component antimicrobial peptide-sensing system (2007) Proceedings of the National Academy of Sciences of the United States of America, 104 (22), pp. 9469-9474. , DOI 10.1073/pnas.0702159104; 
Otto, M., Bacterial sensing of antimicrobial peptides (2009) Contrib Microbiol, 16, pp. 136-149; 
Peschel, A., Vincent Collins, L., Staphylococcal resistance to antimicrobial peptides of mammalian and bacterial origin (2001) Peptides, 22 (10), pp. 1651-1659. , DOI 10.1016/S0196-9781(01)00500-9, PII S0196978101005009; 
Peschel, A., Jack, R.W., Otto, M., Collins, L.V., Staubitz, P., Nicholson, G., Kalbacher, H., Van Strijp, J.A.G., Staphylococcus aureus resistance to human defensins and evasion of neutrophil killing via the novel virulence factor MprF is based on modification of membrane lipids with L-lysine (2001) Journal of Experimental Medicine, 193 (9), pp. 1067-1076. , DOI 10.1084/jem.193.9.1067; 
Cao, M., Helmann, J.D., The Bacillus subtilis Extracytoplasmic-Function σ X Factor Regulates Modification of the Cell Envelope and Resistance to Cationic Antimicrobial Peptides (2004) Journal of Bacteriology, 186 (4), pp. 1136-1146. , DOI 10.1128/JB.186.4.1136-1146.2004; 
Clarke, S.R., Foster, S.J., IsdA protects Staphylococcus aureus against the bactericidal protease activity of apolactoferrin (2008) Infection and Immunity, 76 (4), pp. 1518-1526. , DOI 10.1128/IAI.01530-07; 
Vuong, C., Voyich, J.M., Fischer, E.R., Braughton, K.R., Whitney, A.R., DeLeo, F.R., Otto, M., Polysaccharide intercellular adhesin (PIA) protects Staphylococcus epidermidis against major components of the human innate immune system (2004) Cellular Microbiology, 6 (3), pp. 269-275. , DOI 10.1046/j.1462-5822.2004.00367.x; 
Zasloff, M., Antimicrobial peptides of multicellular organisms (2002) Nature, 415 (6870), pp. 389-395. , DOI 10.1038/415389a; 
Hancock, R.E.W., Peptide antibiotics (1997) Lancet, 349 (9049), pp. 418-422. , DOI 10.1016/S0140-6736(97)80051-7; 
Cho, W.-M., Joshi, B.P., Cho, H., Lee, K.-H., Design and synthesis of novel antibacterial peptide-resin conjugates (2007) Bioorganic and Medicinal Chemistry Letters, 17 (21), pp. 5772-5776. , DOI 10.1016/j.bmcl.2007.08.056, PII S0960894X0701013X; 
Statz, A.R., Park, J.P., Chongsiriwatana, N.P., Barron, A.E., Messersmith, P.B., Surface-immobilised antimicrobial peptoids (2008) Biofouling, 24 (6), pp. 439-448; 
Deslouches, B., Gonzalez, I.A., DeAlmeida, D., Islam, K., Steele, C., Montelaro, R.C., Mietzner, T.A., De novo-derived cationic antimicrobial peptide activity in a murine model of Pseudomonas aeruginosa bacteraemia (2007) Journal of Antimicrobial Chemotherapy, 60 (3), pp. 669-672. , DOI 10.1093/jac/dkm253; 
Novak, K.F., Diamond, W.J., Kirakodu, S., Peyyala, R., Anderson, K.W., Montelaro, R.C., Mietzner, T.A., Efficacy of the de novo-derived antimicrobial peptide WLBU2 against oral bacteria (2007) Antimicrobial Agents and Chemotherapy, 51 (5), pp. 1837-1839. , DOI 10.1128/AAC.00924-06; 
Deslouches, B., Islam, K., Craigo, J.K., Paranjape, S.M., Montelaro, R.C., Mietzner, T.A., Activity of the de novo engineered antimicrobial peptide WLBU2 against Pseudomonas aeruginosa in human serum and whole blood: Implications for systemic applications (2005) Antimicrobial Agents and Chemotherapy, 49 (8), pp. 3208-3216. , DOI 10.1128/AAC.49.8.3208-3216.2005; 
Deslouches, B., Phadke, S.M., Lazarevic, V., Cascio, M., Islam, K., Montelaro, R.C., Mietzner, T.A., De novo generation of cationic antimicrobial peptides: Influence of length and tryptophan substitution on antimicrobial activity (2005) Antimicrobial Agents and Chemotherapy, 49 (1), pp. 316-322. , DOI 10.1128/AAC.49.1.316-322.2005; 
Levin, M., Quint, P.A., Goldstein, B., Barton, P., Bradley, J.S., Shemie, S.D., Recombinant bactericidal/permeability-increasing protein (rBPI21) as adjunctive treatment for children with severe meningococcal sepsis: A randomised trial. rBPI21 Meningococcal Sepsis Study Group (2000) Lancet, 356 (9234), pp. 961-967; 
Giroir, B.P., Scannon, P.J., Levin, M., Bactericidal/permeability-increasing protein-lessons learned from the phase III, randomized, clinical trial of rBPI21 for adjunctive treatment of children with severe meningococcemia (2001) Crit Care Med, 29 (7 SUPPL.), pp. 130-135; 
Brogden, K.A., Antimicrobial peptides: Pore formers or metabolic inhibitors in bacteria? (2005) Nature Reviews Microbiology, 3 (3), pp. 238-250. , DOI 10.1038/nrmicro1098; 
Bastos, M., Bai, G., Gomes, P., Andreu, D., Goormaghtigh, E., Prieto, M., Energetics and partition of two cecropin-melittin hybrid peptides to model membranes of different composition (2008) Biophys J, 94 (6), pp. 2128-2141; 
Melo, M.N., Ferre, R., Castanho, M.A., Antimicrobial peptides: Linking partition, activity and high membrane-bound concentrations (2009) Nat Rev Microbiol, 7 (3), pp. 245-250; 
Haynie, S.L., Crum, G.A., Doele, B.A., Antimicrobial activities of amphiphilic peptides covalently bonded to a water-insoluble resin (1995) Antimicrob Agents Chemother, 39 (2), pp. 301-307; 
Humblot, V., Yala, J.F., Thebault, P., Boukerma, K., Hequet, A., Berjeaud, J.M., The antibacterial activity of Magainin i immobilized onto mixed thiols self-assembled monolayers (2009) Biomaterials, 30 (21), pp. 3503-3512; 
Bagheri, M., Beyermann, M., Dathe, M., Immobilization reduces the activity of surface-bound cationic antimicrobial peptides with no influence upon the activity spectrum (2009) Antimicrob Agents Chemother, 53 (3), pp. 1132-1141; 
Gabriel, M., Nazmi, K., Veerman, E.C., Nieuw Amerongen, A.V., Zentner, A., Preparation of LL-37-grafted titanium surfaces with bactericidal activity (2006) Bioconj Chem, 17 (2), pp. 548-550; 
Hilpert, K., Elliott, M., Jenssen, H., Kindrachuk, J., Fjell, C.D., Korner, J., Screening and characterization of surface-tethered cationic peptides for antimicrobial activity (2009) Chem Biol, 16 (1), pp. 58-69; 
Batista, M.K.S., Gallemi, M., Adeva, A., Gomes, C.A.R., Gomes, P., Facile regioselective synthesis of a novel chitosan-pexiganan conjugate with potential interest for the treatment of infected skin lesions (2009) Synthetic Commun, 39 (7), pp. 1228-1240; 
Venter, J.C., Immobilized and insolubilized drugs, hormones, and neurotransmitters: Properties, mechanisms of action and applications (1982) Pharmacological Reviews, 34 (2), pp. 153-187; 
Endo, Y., Tani, T., Kodama, M., Antimicrobial activity of tertiary amine covalently bonded to a polystyrene fiber (1987) Appl Environ Microbiol, 53 (9), pp. 2050-2055; 
Chuang, H.F., Shukla, A., Loose, C.R., Hammond-Cunningham, P.T., Stephanopoulos, G., (2009) Structures Including Antimicrobial Peptides, , United States; 
Chen, R., Cole, N., Willcox, M.D., Park, J., Rasul, R., Carter, E., Synthesis, characterization and in vitro activity of a surface-attached antimicrobial cationic peptide (2009) Biofouling, 25 (6), pp. 517-524; 
Steven, M.D., Hotchkiss, J.H., Covalent immobilization of an antimicrobial peptide on poly(ethylene) film (2008) J Appl Polym Sci, 110 (5), pp. 2665-2670; 
Appendini, P., Hotchkiss, J.H., Surface modification of poly(styrene) by the attachment of an antimicrobial peptide (2001) J Appl Polym Sci, 81 (3), pp. 609-616; 
Fields, G.B., Noble, R.L., Solid phase peptide synthesis utilizing 9-fluorenylmethoxycarbonyl amino acids (1990) International Journal of Peptide and Protein Research, 35 (3), pp. 161-214; 
Rostovtsev, V.V., Green, L.G., Fokin, V.V., Sharpless, K.B., A stepwise huisgen cycloaddition process: Copper(I)-catalyzed regioselective "ligation" of azides and terminal alkynes (2002) Angewandte Chemie - International Edition, 41 (14), pp. 2596-2599. , DOI 10.1002/1521-3773(20020715)41: 14<2596::AID-ANIE2596>3.0.CO;2-4; 
Abad, J.M., Velez, M., Santamaria, C., Guisan, J.M., Matheus, P.R., Vazquez, L., Gazaryan, I., Fernandez, V.M., Immobilization of peroxidase glycoprotein on gold electrodes modified with mixed epoxy-boronic acid monolayers (2002) Journal of the American Chemical Society, 124 (43), pp. 12845-12853. , DOI 10.1021/ja026658p; 
Lee, M.-R., Shin, I., Fabrication of chemical microarrays by efficient immobilization of hydrazide-linked substances on epoxide-coated glass surfaces (2005) Angewandte Chemie - International Edition, 44 (19), pp. 2881-2884. , DOI 10.1002/anie.200462720; 
Hudalla, G.A., Murphy, W.L., Immobilization of peptides with distinct biological activities onto stem cell culture substrates using orthogonal chemistries (2010) Langmuir, 26 (9), pp. 6449-6456; 
Cole, N., Hume, E.B., Vijay, A.K., Sankaridurg, P., Kumar, N., Willcox, M.D., In vivo performance of melimine as an antimicrobial coating for contact lenses in models of CLARE and CLPU (2010) Invest Ophthalmol Vis Sci, 51 (1), pp. 390-395
},
	month = {2011///},
	pages = {1431 - 1440},
	abstract = {Bacterial adhesion to biomaterials remains a major problem in the medical devices field. Antimicrobial peptides (AMPs) are well-known components of the innate immune system that can be applied to overcome biofilm-associated infections. Their relevance has been increasing as a practical alternative to conventional antibiotics, which are declining in effectiveness. The recent interest focused on these peptides can be explained by a group of special features, including a wide spectrum of activity, high efficacy at very low concentrations, target specificity, anti-endotoxin activity, synergistic action with classical antibiotics, and low propensity for developing resistance. Therefore, the development of an antimicrobial coating with such properties would be worthwhile. The immobilization of AMPs onto a biomaterial surface has further advantages as it also helps to circumvent AMPs{\textquoteright} potential limitations, such as short half-life and cytotoxicity associated with higher concentrations of soluble peptides. The studies discussed in the current review report on the impact of covalent immobilization of AMPs onto surfaces through different chemical coupling strategies, length of spacers, and peptide orientation and concentration. The overall results suggest that immobilized AMPs may be effective in the prevention of biofilm formation by reduction of microorganism survival post-contact with the coated biomaterial. Minimal cytotoxicity and long-term stability profiles were obtained by optimizing immobilization parameters, indicating a promising potential for the use of immobilized AMPs in clinical applications. On the other hand, the effects of tethering on mechanisms of action of AMPs have not yet been fully elucidated. Therefore, further studies are recommended to explore the real potential of immobilized AMPs in health applications as antimicrobial coatings of medical devices. {\textcopyright} 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.},
	keywords = {Amino Acid Sequence, amoxicillin, ampicillin, Animals, antibiotic agent, antibiotic resistance, Antimicrobial Cationic Peptides, Antimicrobial peptides, Bacteria (microorganisms), Bacterial adhesion, bacterial survival, Biocompatible Materials, biofilm, Biofilms, biomaterial, carbenicillin, cefazolin, Coated Materials, Biocompatible, Cytotoxicity, drug activity, drug half life, drug solubility, drug stability, drug targeting, gentamicin, human, Humans, Immobilized Proteins, Molecular Sequence Data, mouth inflammation, Nanostructured surfaces, nonhuman, penicillin G, polypeptide antibiotic agent, priority journal, review, sepsis, skin infection, soft tissue infection, Surface functionalization, Surface Properties, teicoplanin, tobramycin, vancomycin},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-79952187401\&partnerID=40\&md5=70d486a4c6e1ba88f804c0f0d4f4e510},
	author = {Costa, F. and Carvalho, I.F. and Montelaro, R.C. and Gomes, P. and Martins, M.C.L.}
}
@article {2267,
	title = {Cell nuclei and cytoplasm joint segmentation using the sliding band filter},
	journal = {IEEE Transactions on Medical ImagingIEEE Trans. Med. Imaging},
	volume = {29},
	year = {2010},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusArt. No.: 5477157CODEN: ITMIDdoi: 10.1109/TMI.2010.2048253Language of Original Document: EnglishCorrespondence Address: Quelhas, P.; Instituto de Engenharia Biom{\'e}dica (INEB), 4200-465 Porto, Portugal; email: pedro.quelhas@gmail.comChemicals/CAS: DNA, 9007-49-2; Actins; DNA, 9007-49-2References: Bleau, A., Leon, L., Watershed-based segmentation and region merging (2000) Comput. Vis. Image Understand., 77 (3), pp. 317-370;Clocksin, W., Automatic segmentation of overlapping nuclei with high background variation using robust estimation and flexible contour models (2003) Proc. 12th Int. Conf. Image Anal. Process., pp. 682-687; 
Cloppet, F., Boucher, A., Segmentation of overlapping/aggregating nuclei cells in biological images (2008) Proc. 19th Int. Conf. Pattern Recognit. (ICPR 2008), pp. 1-4; 
Danek, O., Matula, P., De Solorzano, C.O., Munoz-Barrutia, A., Maska, M., Kozubek, M., Segmentation of touching cell nuclei using a two-stage graph cut model (2009) LNCS, 5575, pp. 410-419. , Proc. SCIA; 
Fok, Y., Chan, J., Chin, R., Automated analysis of nerve-cell images using active contour models (1996) IEEE Trans. Med. Imag., 15 (3), pp. 353-368. , Jun; 
Harder, N., M-Bermudez, F., Godinez, W., Ellenberg, J., Eils, R., Rohr, K., Automated analysis of mitotic cell nuclei in 3-D fluorescence microscopy image sequences (2008) Workshop Bio-Image Inf.: Biol. Imag., Comput. Vis. Data Mining, pp. 17-18. , Santa Barbara, CA; 
Hill, A., Lapan, P., Li, Y., Haney, S., Impact of image segmentation on high-content screening data quality for sk-br-3 cells (2007) BMC Bioin-formatics, 8 (340), pp. 1-13; 
Hu, M., Ping, X., Ding, Y., A new active contour model and its application on cell segmentation (2004) 2004 8th International Conference on Control, Automation, Robotics and Vision (ICARCV), 2, pp. 1104-1107. , TuA7.09 - P0673, 2004 8th International Conference on Control, Automation, Robotics and Vision (ICARCV); 
Jones, T.R., Carpenter, A., Golland, P., Voronoi-based segmentation of cells on image manifolds (2005) ICCV Workshop Comput. Vis. Biomed. Image Appl. (CVBIA), pp. 535-543; 
Klemencic, A., Kovacic, S., Pernus, F., Automated segmentation of muscle fiber images using active contour models (1998) Cytometry, 32, pp. 317-326; 
Kobatake, H., Hashimoto, S., Convergence index filter for vector fields (1999) IEEE Trans. Image Process., 8 (8), pp. 1029-1038. , Aug; 
Lehmussola, A., Ruusuvuori, P., Selinummi, J., Huttunen, H., Yli-Harja, O., Computational framework for simulating fluorescence microscope images with cell populations (2007) IEEE Trans. Med. Imag., 26 (7), pp. 1010-1016. , Jul; 
Luc, V., Soille, P., Watersheds in digital spaces: An efficient algorithm based on immersion simulations (1991) IEEE Trans. Pattern Anal. Mach. Intell., 13 (6), pp. 583-598. , Jun; 
Marcuzzo, M., Guichard, T., Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., Cell division detection on the arabidopsis thaliana root (2009) LNCS, 5524, pp. 168-175. , Proceedings of IbPRIA. New York: Springer; 
Marcuzzo, M., Quelhas, P., Mendon{\c c}a, A.M., Campilho, A., Evaluation of symmetry enhanced sliding band filter for plant cell nuclei detection in low contrast noisy fluorescent images (2009) LNCS, 5627, pp. 824-831. , Proceedings of ICIAR. New York: Springer; 
Moore, P., Molloy, D., A survey of computer-based deformable models (2007) Int. Mach. Vis. Image Process. Conf. (IMVIP), pp. 55-66; 
Otsu, N., A threshold selection method from gray-level histograms (1979) IEEE Tran. Syst., Man, Cybern., 9 (1), pp. 62-66. , Jan; 
Pereira, C.S., Fernandes, H., Mendon{\c c}a, A.M., Campilho, A., Detection of lung nodule candidates in chest radiographs (2007) LNCS, 4478, pp. 170-177; 
Pereira, C.S., Mendon{\c c}a, A.M., Campilho, A., Evaluation of contrast enhancement filters for lung nodule detection Proceedings of ICIAR. New York: Springer, 4633, pp. 878-888; 
Quelhas, P., Marcuzzo, M., Oliveira, M.J., Mendon{\c c}a, A.M., Campilho, A., Cancer cell detection and invasion depth estimation in brightfield images (2009) Proc. 20th Br. Mach. Vis. Conf. (BMVC), , London, U.K; 
Ruusuvuori, P., Lehmussola, A., Selinummi, J., Rajala, T., Huttunen, H., Yli-Harja, O., Benchmark set of synthetic images for validating cell image analysis algorithms (2008) Proc. 16th Eur. Signal Process. Conf., , Lausanne, Switzerland; 
Solorzano, C., Malladi, R., Lelievre, S., Lockett, S., Segmentation of nuclei and cells using membrane related protein markers (2001) J. Microscopy, 201, pp. 404-415; 
Tek, F.B., Dempster, A.G., Kale, I., (2006) Mathematical Morphology:40 Years on, 30, pp. 441-454. , New York: Springer; 
Wei, J., Hagihara, Y., Kobatake, H., Detection of rounded opacities on chest radiographs using convergence index filter (1999) Proc. Int. Conf. Image Anal. Process., pp. 757-761; 
Xiong, G., Zhou, X., Ji, L., Bradley, P., Perrimon, N., Wong, S., Segmentation of drosophila RNAI fluorescence images using level sets (2006) Proc. IEEE Int. Conf. Image Process., pp. 73-76; 
Xiong, G., Zhou, X., Ji, L., Automated segmentation of drosophila RNAi fluorescence cellular images using deformable models (2006) IEEE Trans. Circ. Syst. i, 53 (11), pp. 2415-2424. , Nov; 
Yan, P., Zhou, X., Shah, M., Wong, S.T.C., Automatic segmentation of high-throughput RNAi fluorescent cellular images (2008) IEEE Trans. Inf. Technol. Biomed., 12 (1), pp. 109-117. , Jan; 
Yang, X., Li, H., Zhou, X., Nuclei segmentation using marker-controlled watershed, tracking using mean-shift, and kalman filter in time-lapse microscopy (2006) IEEE Trans. Circuits Syst., 53 (11), pp. 2405-2414. , Nov
},
	month = {2010///},
	pages = {1463 - 1473},
	abstract = {Microscopy cell image analysis is a fundamental tool for biological research. In particular, multivariate fluorescence microscopy is used to observe different aspects of cells in cultures. It is still common practice to perform analysis tasks by visual inspection of individual cells which is time consuming, exhausting and prone to induce subjective bias. This makes automatic cell image analysis essential for large scale, objective studies of cell cultures. Traditionally the task of automatic cell analysis is approached through the use of image segmentation methods for extraction of cells{\textquoteright} locations and shapes. Image segmentation, although fundamental, is neither an easy task in computer vision nor is it robust to image quality changes. This makes image segmentation for cell detection semi-automated requiring frequent tuning of parameters. We introduce a new approach for cell detection and shape estimation in multivariate images based on the sliding band filter (SBF). This filter{\textquoteright}s design makes it adequate to detect overall convex shapes and as such it performs well for cell detection. Furthermore, the parameters involved are intuitive as they are directly related to the expected cell size. Using the SBF filter we detect cells{\textquoteright} nucleus and cytoplasm location and shapes. Based on the assumption that each cell has the same approximate shape center in both nuclei and cytoplasm fluorescence channels, we guide cytoplasm shape estimation by the nuclear detections improving performance and reducing errors. Then we validate cell detection by gathering evidence from nuclei and cytoplasm channels. Additionally, we include overlap correction and shape regularization steps which further improve the estimated cell shapes. The approach is evaluated using two datasets with different types of data: a 20 images benchmark set of simulated cell culture images, containing 1000 simulated cells; a 16 images Drosophila melanogaster Kc167 dataset containing 1255 cells, stained for DNA and actin. Both image datasets present a difficult problem due to the high variability of cell shapes and frequent cluster overlap between cells. On the Drosophila dataset our approach achieved a precision/recall of 95\%/69\% and 82\%/90\% for nuclei and cytoplasm detection respectively and an overall accuracy of 76\%. {\textcopyright} 2010 IEEE.},
	keywords = {actin, Actins, animal, Animals, article, Band filters, Biological research, cell aggregation, Cell analysis, Cell culture, Cell detection, Cell images, cell nucleus, Cell segmentation, Cell Shape, Cell shapes, Cell size, Cells, chemistry, Computer vision, Convergence filters, Convex shapes, cytoplasm, Data sets, Databases, Factual, Digital image storage, DNA, Drosophila melanogaster, factual database, Fluorescence, Fluorescence microscopy, Fundamental tools, High variability, Image analysis, Image datasets, image processing, image processing in biology, Image Processing, Computer-Assisted, Image quality, Image segmentation, Imaging systems, Improving performance, Individual cells, Joint segmentation, Location, methodology, Microscopy, Fluorescence, Multivariate analysis, multivariate image processing, New approaches, Nuclear detection, Overlap correction, reproducibility, Reproducibility of Results, Semi-automated, Shape estimation, Shape regularization, ultrastructure, Visual communication, Visual inspection},
	isbn = {02780062 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77955339493\&partnerID=40\&md5=f8cea406f7b2fcf901dc34fa940522b1},
	author = {Quelhas, P. and Marcuzzo, M. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2270,
	title = {Ceramics for dental restorations - an introduction},
	journal = {Una perspectiva de las cer{\'a}micas para restauraciones dentalesDYNADYNA},
	volume = {77},
	year = {2010},
	note = {Export Date: 21 November 2012Source: ScopusLanguage of Original Document: English; SpanishCorrespondence Address: Santander, S. A.; Universidad Pontificia Bolivariana and Universidad Cooperativa de Colombia, Medell{\'\i}n, Colombia; email: santiago.arango75@gmail.comReferences: Rosenblum, M.A., Schulman, A., A review of allceramic restorations (1997) J Am Dent Assoc, 128 (3), pp. 297-307;Rizkalla, A.S., Jones, D.W., Mechanical properties of commercial high strength ceramic core materials (2004) Dent Mater, 20 (2), pp. 207-212; 
Rizkalla, A.S., Jones, D.W., Indentation fracture toughness and dynamic elastic moduli for commercial feldspathic dental porcelain materials (2004) Dent Mater, 20 (2), pp. 198-206; 
Jones, D.W., Development of dental ceramics. An historical perspective (1985) Dent Clin North Am, 29 (4), pp. 621-644; 
Kelly, J.R., Nishimura, I., Campbell, S.D., Ceramics in dentistry: Historical roots and current perspectives (1996) J Prosthet Dent, 75 (1), pp. 18-32; 
Stejskal, V.D., Metalspecific lymphocytes: Biomarkers of sensitivity in man (1999) Neuro Endocrinol Lett, 20 (5), pp. 289-298; 
Arvidson, K., Wroblewski, R., Migration of metallic ions from screwposts into dentin and surrounding tissues (1978) Scand J Dent Res, 86 (3), pp. 200-205; 
Venclikova, Z., Metallic pigmentation of human teeth and gingiva: Morphological and immunological aspects (2007) Dent Mater J, 26 (1), pp. 96-104; 
Bumgardner, J.D., Lucas, L.C., Cellular response to metallic ions released from nickelchromium dental alloys (1995) J Dent Res, 74 (8), pp. 15-217; 
Mehulic, K., The release of metal ions in the gingival fluid of prosthodontic patients (2005) Acta Stomatol Croat, 39 (1), pp. 47-51; 
Mart{\'\i}nez, F., Cer{\'a}micas dentales: Clasificaci{\'o}n y criterios de selecci{\'o}n (2007) RCOE, 12 (4), pp. 253-263; 
Weinstein, M., Weinstein, L., Weinstein, A., (1962) Porcelain Coveredmetal Reinforced Teeth; 
Anusavice, K.J., Degradability of dental ceramics (1992) Adv Dent Res, 6, p. 829; 
Knorpp, E.A., (1991) Control Arrangement for Dental Furnaces, Especially Microprocessorcontrolled Preheating Furnaces; 
Anusavice, K.J., (2004) Phillips Ciencia De Los Materiales Dentales, , Und{\'e}cima ed: Elsevier Espa{\~n}a; 
Meyer, J.M., O{\textquoteright}Brien, W.J., Yu, C.U., Sintering of dental porcelain enamels (1976) J Dent Res, 55 (4), pp. 696-699; 
Denry, I.L., Recent advances in ceramics for dentistry (1996) Crit Rev Oral Biol Med, 7 (2), pp. 134-143; 
Campbell, S.D., Dimensional and formation analysis of a restorative ceramic and how it works (1995) J Prosthet Dent, 74 (4), pp. 332-340; 
Conrad, H.J., Seong, W.J., Pesun, I.J., Current ceramic materials and systems with clinical recommendations: A systematic review (2007) J Prosthet Dent, 98 (5), pp. 389-404; 
Tinschert, J., Structural reliability of alumina, feldspar, leucite, micaand zirconiabased ceramics (2000) J Dent, 28 (7), pp. 529-535; 
Rahaman, M.N., (2003) Ceramic Processing and Sintering, , materials engineering, Second ed: CRC Press; 
Anusavice, K.J., Hojjatie, B., Effect of thermal tempering on strength and crack propagation behavior of feldspathic porcelains (1991) J Dent Res, 70 (6), pp. 1009-1013; 
Anusavice, K.J., Shen, C., Lee, R.B., Strengthening of feldspathic porcelain by ion exchange and tempering (1992) J Dent Res, 71 (5), pp. 113-148; 
Dunn, B., Levy, M.N., Reisbick, M.H., Improving the fracture resistance of dental ceramic (1977) J Dent Res, 56 (10), pp. 1209-1213; 
Rosa, V., Effect of ion exchange on strength and slow crack growth of a dental porcelain (2009) Dent Mater, 25 (6), pp. 736-743; 
Seha, M.M., Effect of TufCoat on Feldspathic porcelain materials (2005) J Oral Rehabil, 32 (1), pp. 39-45; 
Anusavice, K.J., Gray, A., Shen, C., Influence of initial flaw size on crack growth in airtempered porcelain (1991) J Dent Res, 70 (2), pp. 131-136; 
Asaoka, K., Kuwayama, N., Tesk, J.A., Influence of tempering method on residual stress in dental porcelain (1992) J Dent Res, 71 (9), pp. 1623-1627; 
Dehoff, P.H., Anusavice, K.J., Vontivillu, S.B., Analysis of tempering stresses in metalceramic disks (1996) J Dent Res, 75 (2), pp. 743-751; 
Cesar, P.F., Correlation between fracture toughness and leucite content in dental porcelains (2005) J Dent, 33, pp. 721-729; 
Bertolotti, R.L., Calculation of interfacial stress in porcelainfusedtometal systems (1980) J Dent Res, 59 (11), pp. 1972-1977; 
Nielsen, J.P., Tuccillo, J.J., Calculation of interfacial stress in dental porcelain bonded to gold alloy substrate (1972) J Dent Res, 51 (4), pp. 104-137; 
Fairhurst, C.W., Porcelainmetal thermal compatibility (1981) J Dent Res, 60 (4), pp. 815-819; 
Vasquez, V., Mechanical and thermal cycling effects on the flexural strength of glass ceramics fused to titanium (2008) Dent Mater J, 27 (1), pp. 7-15; 
Sheu, T., Mechanical properties and thermal expansion behaviour in leucite containing materials (1994) J Mater Sci, 29, pp. 125-128; 
Denry, I.L., Effect of cubic leucite stabilization on the flexural strength of feldspathic dental porcelain (1996) J Dent Res, 75 (12), pp. 1928-1935; 
Pinto, M.M., Influence of pH on slow crack growth of dental porcelains (2008) Dent Mater, 24 (6), pp. 814-823; 
McLean, J.W., Evolution of dental ceramics in the twentieth century (2001) J Prosthet Dent, 85 (1), pp. 61-66; 
Sherrill, C.A., O{\textquoteright}Brien, W.J., Transverse strength of aluminous and feldspathic porcelain (1974) J Dent Res, 53, pp. 683-690; 
Alshehri, S., Relative fracture toughness and hardness of dental ceramics (2002) Saudi Dent J, 14 (2), pp. 67-72; 
Chaiyabutr, Y., Giordano, R., Pober, R., The effect of different powder particle size on mechanical properties of sintered alumina, resinand glassinfused alumina (2009) J Biomed Mater Res B Appl Biomater, 88 (2), pp. 502-508; 
Alvarezfern{\'a}ndez, M.A., Caracter{\'\i}sticas generales y propiedades de las cer{\'a}micas sin metal (2003) RCOE, 8 (5), pp. 525-546; 
Sorensen, J.A., InCeram fixed partial dentures: Threeyear clinical trial results (1998) J Calif Dent Assoc, 26 (3), pp. 207-214; 
Tyszblat, M., (1988) Process for the Preparation of a Dental Prosthesis By Slight Solid Phase Fritting of a Metal Oxide Based Infrastructure; 
Andersson, M., Oden, A., A new allceramic crown. A densesintered, highpurity alumina coping with porcelain (1993) Acta Odontol Scand, 51 (1), pp. 59-64; 
Andersson, M., Clinical results with titanium crowns fabricated with machine duplication and spark erosion (1989) Acta Odontol Scand, 47 (5), pp. 279-286; 
Oilo, G., Flexural strength and internal defects of some dental porcelains (1988) Acta Odontol Scand, 46 (5), pp. 313-322; 
Pel{\'a}ezvargas, A., Microhardness obtained with two ceramic paste preparation methods (2007) J Dent Res, 87 (Spec Iss B), p. 0294; 
Drummond, J.L., Mechanical property evaluation of pressable restorative ceramics (2000) Dent Mater, 16 (3), pp. 226-233; 
Gonzaga, C.C., Mechanical properties and porosity of dental glassceramics hotpressed at different temperatures (2008) Mat Res, 11 (3), pp. 301-306; 
Ardlin, B.I., Transformationtoughened zirconia for dental inlays, crowns and bridges: Chemical stability and effect of lowtemperature aging on flexural strength and surface structure (2002) Dent Mater, 18 (8), pp. 590-595; 
Vleugels, J., Yuan, Z.X., van der Biest, O., Mechanical properties of Y2O3/Al2O3coated YTZP ceramics (2002) J Eur Ceram Soc, 22, pp. 873-881; 
Craig, R.G., Peyton, F.A., The microharness of enamel and dentin (1958) J Dent Res, 37, pp. 661-668; 
Hassan, R., Caputo, A.A., Bunshah, R.F., Fracture toughness of human enamel (1981) J Dent Res, 60 (4), pp. 820-827; 
Rasmussen, S.T., Patchin, R.E., Fracture properties of human enamel and dentin in an aqueous environment (1984) J Dent Res, 63 (12), pp. 1362-1368; 
Fuentes, V., Microhardness of superficial and deep sound human dentin (2003) J Biomed Mater Res A, 66 (4), pp. 850-853; 
el Mowafy, O.M., Watts, D.C., Fracture toughness of human dentin (1986) J Dent Res, 65 (5), pp. 677-681; 
(1982) Glassceramic Dental Products; 
(1973) Tetrasilicic Mica Glassceramic Method; 
(1956) Method of Making Ceramics and Products Thereof; 
Denry, I.L., Rosenstiel, S.F., Flexural strength and fracture toughness of Dicor glassceramic after embedment modification (1993) J Dent Res, 72 (3), pp. 572-576; 
Thompson, J.Y., Fracture surface characterization of clinically failed allceramic crowns (1994) J Dent Res, 73 (12), pp. 1824-1832; 
Elmowafy, O., Brochu, J.F., Longevity and clinical performance of IPSEmpress ceramic restorationsa literature review (2002) J Can Dent Assoc, 68 (4), pp. 233-237; 
Probster, L., In vitro evaluation of a glassceramic restorative material (1997) J Oral Rehabil, 24 (9), pp. 636-645; 
Sorensen, J.A., IPS Empress crown system: Threeyear clinical trial results (1998) J Calif Dent Assoc, 26 (2), pp. 130-136; 
(1990) Dispersion Strengthened Composite; 
Fischer, H., Marx, R., Improvement of strength parameters of a leucitereinforced glass ceramic by dualion exchange (2001) J Dent Res, 80 (1), pp. 336-339; 
Mansour, Y.F., Clinical performance of IPSEmpress 2 ceramic crowns inserted by general dental practitioners (2008) J Contemp Dent Pract, 9 (4), pp. 9-16; 
Brodkin, D., Panzera, C., Panzera, P., (2002) Pressable Lithium Disilicate Glass Ceramics; 
Garvie, R.C., Hannink, R.H., Pascoe, R.T., Ceramic steel? (1975) Nature, 258 (5537), pp. 703-704; 
Piconi, C., Maccauro, G., Zirconia as a ceramic biomaterial (1999) Biomaterials, 20 (1), pp. 1-25; 
Guazzato, M., Strength, fracture toughness and microstructure of a selection of allceramic materials (2004) Zirconiabased Dental Ceramics, Dent Mater, 20 (5), pp. 449-456. , Part II; 
Pilathadka, S., Vahalova, D., Vosahlo, T., The Zirconia: A new dental ceramic material (2007) An Overview, Prague Med Rep, 108 (1), pp. 5-12; 
Deville, S., Lowtemperature ageing of zirconiatoughened alumina ceramics and its implication in biomedical implants (2003) J Eur Ceram Soc, 23, pp. 2975-2982; 
Zivkobabic, J., Carek, A., Jakovac, M., Zirconium oxide ceramics in prosthodontics (2005) Acta Stomatol Croat, 38 (1), pp. 25-28; 
Piconi, C., YTZP ceramics for artificial joint replacements (1998) Biomaterials, 19 (16), pp. 1489-1494; 
Chemical synthesis of ceramic materials (1997) J Mater Chem, 7 (8), pp. 1297-1305
},
	month = {2010///},
	pages = {26 - 36},
	abstract = {Dental ceramics are the preferred materials for oral restoration due to some characteristics, such as adequate esthetics, high fracture strength and chemical stability. Currently, dental professionals have a large amount of ceramic systems to choose from, all of them having small differences regarding their chemistry, processing temperatures, mechanical strength and clinical applications. These differences lead to classification systems that are difficult to understand by professionals outside the dental field. The aim of this work is to review the current dental ceramic systems and present them from a compositional perspective to assure the comprehension of these materials by professionals who belong to the Biomedical Engineering field. A great effort was made to avoid classification methods imposed by manufacturers and to obtain a quick compilation of available information from the literature allowing the reader to have a general view of the stateoftheart on dental ceramics.},
	keywords = {Ceramic systems, Feldspathic porcelain, Glassceramics, Leucite, Mechanical and physical properties, Zirconium oxide},
	isbn = {00127353 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-80051564798\&partnerID=40\&md5=2e79174a5238798dfcce9d2b5d7f29e0},
	author = {Santander, S.A. and Vargas, A.P. and Escobar, J.S. and Monteiro, F.J. and Tamayo, L.F.R.}
}
@article {2274,
	title = {Characterization of polymeric solutions as injectable vehicles for hydroxyapatite microspheres},
	journal = {AAPS PharmSciTechAAPS PharmSciTech},
	volume = {11},
	year = {2010},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: Scopusdoi: 10.1208/s12249-010-9447-3PubMed ID: 20490958Language of Original Document: EnglishCorrespondence Address: Oliveira, S. M.; Dep. de Eng. Mec{\^a}nica e Gest{\~a}o Industrial, Escola Superior de Tecnologia e Gest{\~a}o de Viseu, Campus Polit{\'e}cnico de Repeses, 3504-510, Viseu, Portugal; email: soliveira@estv.ipv.ptChemicals/CAS: alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; carboxymethylcellulose, 8050-38-2, 9000-11-7, 9004-32-4, 9050-04-8; hydroxyapatite, 1306-06-5, 51198-94-8; hydroxypropylmethylcellulose, 9004-65-3; Biocompatible Materials; Capsules; Durapatite, 1306-06-5; Excipients; Polymers; Solutions; VehiclesReferences: Verlaan, J.J., Oner, F.C., Dhert, W.J., Anterior spinal column augmentation with injectable bone cements (2006) Biomat, 27 (3), pp. 290-301;Chenite, A., Chaput, C., Wang, D., Combes, C., Buschmann, M.D., Hoemann, C.D., Novel injectable neutral solutions of chitosan form biodegradable gels in situ (2000) Biomat, 21 (21), pp. 2155-2161; 
Hoemann, C.D., Sun, J., Legare, A., McKee, M.D., Buschmann, M.D., Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell-delivery vehicle (2005) Osteoarthritis Cart., 13 (4), pp. 318-329; 
Balakrishnan, B., Jayakrishnan, A., Self-cross-linking biopolymers as injectable in situ forming biodegradable scaffolds (2005) Biomat, 26 (18), pp. 3941-3951; 
Shu, X.Z., Ghosh, K., Liu, Y., Palumbo, F.S., Luo, Y., Clark, R.A., Attachment and spreading of fibroblasts on an RGD peptidemodified injectable hyaluronan hydrogel (2004) J. Biomed. Mater. Res., 68 A (2), pp. 365-375; 
Shu, X.Z., Ahmad, S., Liu, Y., Prestwich, G.D., Synthesis and evaluation of injectable, in situ crosslinkable synthetic extracellular matrices for tissue engineering (2006) J. Biomed. Mater. Res. A, 79 (4), pp. 902-912; 
Weiss, P., Gauthier, O., Bouler, J.-M., Grimandi, G., Daculsi, G., Injectable bone substitute using a hydrophilic polymer (1999) Bone, 25 (SUPPL. 1), pp. 67S-70S. , DOI 10.1016/S8756-3282(99)00146-5, PII S8756328299001465; 
Trojani, C., Weiss, P., Michiels, J.F., Vinatier, C., Guicheux, J., Daculsi, G., Three-dimensional culture and differentiation of human osteogenic cells in an injectable hydroxypropylmethylcellulose hydrogel (2005) Biomat, 26 (27), pp. 5509-5517; 
Virto, M.R., Frutos, P., Torrado, S., Frutos, G., Gentamicin release from modified acrylic bone cements with lactose and hydroxypropylmethylcellulose (2003) Biomat, 24 (1), pp. 79-87; 
Bodic, F., Amouriq, Y., Gayet-Delacroix, M., Gauthier, O., Bouler, J.-M., Daculsi, G., M{\'e}thode nom invasive d{\textquoteright}evaluation d{\textquoteright}un substitut osseux injectable/Non-invasive evaluation of an injectable bone substitute (2002) C R Biologies, 325, pp. 345-353; 
Andrews, G.P., Gorman, S.P., Jones, D.S., Rheological characterisation of primary and binary interactive bioadhesive gels composed of cellulose derivatives designed as ophthalmic viscosurgical devices (2005) Biomat, 26 (5), pp. 571-580; 
He, S., Yaszemski, M.J., Yasko, A.W., Engel, P.S., Mikos, A.G., Injectable biodegradable polymer composites based on poly (propylene fumarate) crosslinked with poly (ethylene glycol) dimethacrylate (2000) Biomat, 21 (23), pp. 2389-2394; 
Temenoff, J.S., Mikos, A.G., Injectable biodegradable materials for orthopedic tissue engineering (2000) Biomat, 21 (23), pp. 2405-2412; 
Iooss, P., Le Ray, A.M., Grimandi, G., Daculsi, G., Merle, C., A new injectable bone substitute combining poly (epsilon-caprolactone) microparticles with biphasic calcium phosphate granules (2001) Biomat, 22 (20), pp. 2785-2794; 
Uda, H., Sugawara, Y., Nakasu, M., Experimental studies on hydroxyapatite powder-carboxymethyl chitin composite: Injectable material for bone augmentation (2006) J. Plast. Reconstr. Aesthet. Surg., 59 (2), pp. 188-196; 
Carrodeguas, R.G., Lasa, B.V., Barrio, J.S.D., Injectable acrylic bone cements for vertebroplasty with improved properties (2004) J. Biomed. Mater. Res. B-Appl. Biomat., 68 (1), pp. 94-104; 
Oliveira, S.M., Barrias, C.C., Ribeiro, C.C., Almeida, I.F., Bahia, M.F., Barbosa, M.A., Morphology and mechanical properties of injectable ceramic microspheres (2009) Key Engineering Mat., 396-398, pp. 691-694; 
Barrias, C.C., Ribeiro, C.C., Barbosa, M.A., Adhesion and proliferation of human osteoblastic cells seeded on injectable hydroxyapatite microspheres (2004) Key Engineering Mat., 254-256, pp. 877-880; 
Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., Preparation and characterisation of calcium-phosphate porous microspheres with a uniform size for biomedical applications (2006) J. Mater. Sci. Mater. Med., 17 (5), pp. 455-463; 
Oliveira, S.M., Barrias, C.C., Almeida, I.F., Costa, P.C., Ferreira, M.P., Bahia, M.F., Injectability of a bone filler system based on hydroxyapatite microspheres and a vehicle with in situ gelforming ability (2008) J. Biomed. Mater. Res. B-Appl. Biomat., 87 B, pp. 49-58; 
Barnes, H.A., Hutton, J.F., Walters, K., (1998) An Introduction to Rheology, , Amsterdam: Elsevier Science; 
Leo, W.J., McLoughlin, A.J., Malone, D.M., Effects of sterilization treatments on some properties of alginate solutions and gels (1990) Biotech. Progress, 6 (1), pp. 51-53; 
Holme, H.K., Lindmo, K., Kristiansen, A., Smidsr{\o}d, O., Thermal depolymerization of alginate in the solid state (2003) Carbohydrate Polym., 54 (4), pp. 431-438; 
Bohner, M., Baroud, G., Injectability of calcium phosphate pastes (2005) Biomat, 26 (13), pp. 1553-1563; 
Hide, I.G., Gangi, A., Percutaneous vertebroplasty: History, technique and current perspectives (2004) Clin. Radiol., 59 (6), pp. 461-467; 
Lewis, G., Injectable bone cements for use in vertebroplasty and kyphoplasty: State-of-the-art review (2006) J. Biomed. Mat. Res. B-Appl. Biomat., 76 B (2), pp. 456-468; 
Baumann, A., Tauss, J., Baumann, G., Tomka, M., Hessinger, M., Tiesenhausen, K., Cement embolization into the vena cava and pulmonal arteries after vertebroplasty: Interdisciplinary management (2006) Europ J. Vasc. and End Surg., 31 (5), pp. 558-561; 
Mathis, J.M., Wong, W., Percutaneous vertebroplasty: Technical considerations (2003) J. Vasc. and Intervent Rad., 14 (8), pp. 953-960; 
Xu, H.H., Weir, M.D., Burguera, E.F., Fraser, A.M., Injectable and macroporous calcium phosphate cement scaffold (2006) Biomat, 27 (24), pp. 4279-4287; 
Gisep, A., Curtis, R., Hanni, M., Suhm, N., Augmentation of implant purchase with bone cements: An in vitro study of injectability and dough distribution (2006) J. Biomed. Mater. Res. B-Appl. Biomat., 77 (1), pp. 114-119; 
Krebs, J., Ferguson, S.J., Bohner, M., Baroud, G., Steffen, T., Heini, P.F., Clinical measurements of cement injection pressure during vertebroplasty (2005) Spine, 30 (5), pp. E118-E122; 
Draget, K.I., Skjak-Braek, G., Smidsrod, O., Alginate-based new materials (1997) Int. J. Biol. Macromol., 21 (1-2), pp. 47-55
},
	month = {2010///},
	pages = {852 - 858},
	abstract = {A polymeric solution and a reinforcement phase can work as an injectable material to fill up bone defects. However, the properties of the solution should be suitable to enable the transport of that extra phase. Additionally, the use of biocompatible materials is a requirement for tissue regeneration. Thus, we intended to optimize a biocompatible polymeric solution able to carry hydroxyapatite microspheres into bone defects using an orthopedic injectable device. To achieve that goal, polymers usually regarded as biocompatible were selected, namely sodium carboxymethylcellulose, hydroxypropylmethylcellulose, and Na-alginate (ALG). The rheological properties of the polymeric solutions at different concentrations were assessed by viscosimetry before and after moist heat sterilization. In order to correlate rheological properties with injectability, solutions were tested using an orthopedic device applied for minimal invasive surgeries. Among the three polymers, ALG solutions presented the most suitable properties for our goal and a non-sterile ALG 6\% solution was successfully used to perform preliminary injection tests of hydroxyapatite microspheres. Sterile ALG 7.25\% solution was found to closely match non-sterile ALG 6\% properties and it was selected as the optimal vehicle. Finally, sterile ALG 7.25\% physical stability was studied at different temperatures over a 3-month period. It was observed that its rheological properties presented minor changes when stored at 25{\textdegree}C or at 4{\textdegree}C. {\textcopyright} 2010 American Association of Pharmaceutical Scientists.},
	keywords = {Alginate, alginic acid, article, Biocompatibility, Biocompatible Materials, Capsules, Carboxymethylcellulose, Drug Compounding, Durapatite, Excipients, hydroxyapatite, Hydroxypropylmethylcellulose, Injectability, injection, Injections, instrument sterilization, microsphere, Microspheres, minimally invasive surgery, molecular weight, orthopedic equipment, Polymers, priority journal, Solutions, Vehicles, viscometry},
	isbn = {15309932 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77954819260\&partnerID=40\&md5=e8503462fa39dcc285aef7e07f765eef},
	author = {Oliveira, S.M. and Almeida, I.F. and Costa, P.C. and Barrias, C.C. and Ferreira, M.R.P. and Bahia, M.F. and Barbosa, M.A.}
}
@article {2265,
	title = {Characterization of two DLC coatings for joint prosthesis: The role of albumin on the tribological behavior},
	journal = {Surface and Coatings TechnologySurf. Coat. Technol.},
	volume = {204},
	year = {2010},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.surfcoat.2010.04.022Language of Original Document: EnglishCorrespondence Address: Serro, A.P.; Centro de Qu{\'\i}mica Estrutural, Complexo I, Instituto Superior T{\'e}cnico, Av. Rovisco Pais, TU Lisbon, 1049-001 Lisboa, Portugal; email: anapaula.serro@ist.utl.ptReferences: Donnet, C., Erdemir, A., (2009), Springer Science \& Business Media, New YorkGutensohn, K., Beythien, C., Bau, J., Fenner, T., Grewe, P., Koester, R., Padmanaban, K., Kuehnl, P., (2000) Thromb. Res., 99, p. 577;Cadosch, D., Chan, E., Gautschi, O.P., Simmen, H.-P., Filgueira, L., (2009) J. Orthop. Res., 27, p. 841; 
Gillespie, W.L., Frampton, C.M.A., Henderson, R.J., Ryan, P.M., (1988) J. Bone Joint Surg. Br., 70 (4), p. 539; 
Roy, R.K., Lee, K.-R., (2007) J. Biomed. Mater. Res. B Appl. Biomater., 83 B (1), p. 72; 
Alakoski, E., Tiainen, V.-M., Soininen, A., Konttinen, Y.T., (2008) Open Orthop. J., 2, p. 43; 
Fisher, J., Firkins, P., Reeves, E.A., Hailey, J.L., Isaac, G.H., (1995) Proc. Inst. Mech. Eng., 209, p. 263; 
Sheeja, D., Tay, B.K., Lau, S.P., Nung, L.N., (2001) Surf. Coat. Technol., p. 410; 
Tiainen, V.-M., (2001) Diamond Relat. Mater., 10 (2), p. 153; 
O{\~n}ate, J.I., Comin, M., Braceras, I., Garcia, A., Viviente, J.L., Brizuela, M., Garagorri, N., Alava, J.I., (2001) Surf. Coat. Technol., p. 1056; 
Shi, B., Ajayi, O.O., Fenske, G., Erdemir, A., Liang, H., (2003) Wear, 255, p. 1015; 
Lappalainen, R., Selenius, M., Anttila, A., Konttinen, Y.T., Santavirta, S.S., (2003) J. Appl. Biomater., 66 B, p. 410; 
Sheeja, D., Tay, B.K., Nung, L.N., (2004) Diamond Relat. Mater., 13, p. 184; 
Xu, T., Pruitt, L., (1999) J. Mater. Sci. Mater. Med., 10, p. 83; 
Lappalainen, R., Anttila, A., Heinonen, H., (1998) Clin. Orthop., 352, p. 118; 
Saikko, V., Ahlroos, T., Calonius, O., Ker{\"a}nen, J., (2001) Biomaterials, 22, p. 1507; 
Sheeja, D., Tay, B.K., Lau, S.P., Nung, L.N., (2001) Surf. Coat. Technol., 146, p. 410; 
Affatato, S., Frigo, M., Toni, A., (2000) J. Biomed. Mater. Res., 53 (3), p. 221; 
Scholes, S.C., Unsworth, A., Goldsmit, A.A.J., (2000) Phys. Med. Biol., 45, p. 3721; 
Ahlroos, T., Saikko, V., (1997) Wear, 211, p. 113; 
Fedel, M., Motta, A., Maniglio, D., (2009) J. Biomed. Mater. Res., 90 B (1), p. 338; 
Lousiniana, S., Logothetidis, S., Laskarakisa, A., Gioti, M., (2007) Biomolec. Eng., 24 (1), p. 107; 
Li, B.G., Kang, Y.Q., Yin, G.F., Zheng, C.Q., (2007) Key Eng. Mater. High-Performance Ceram. IV, p. 1814; 
Fenske, G.R., (2009) Presented to Chicago Section STLE meeting, , M; 
Eryilmaz, O.L., Erdemir, A., (2008) Surf. Coat. Technol., 203, p. 750; 
Gerwin, N., Hops, C., Lucke, A., (2006) Adv. Drug Deliv. Rev., 58 (2), p. 226; 
Visco, A.M., Torrisi, L., Campo, N., Emanuele, U., Trifiro, A., Trimarchi, M., (2009) J. Biomed. Mater. Res. B Appl. Biomater., 89 B, p. 55; 
Ferrari, A.C., Robertson, J., (2001) Phys. Rev. B, 64, p. 75414; 
Ferrari, A.C., Robertson, J., (2000) Phys. Rev. B, 61, p. 14095; 
Zhou, B., Prorok, B.C., Erdemir, A., Eryilmaz, O., (2007) Diamond Relat. Mater., 16, p. 342; 
Zhou, B., Prorok, B.C., Erdemir, A., Eryilmaz, O., (2006) Diamond Relat. Mater., 15, p. 2051; 
Beeman, D., Silverman, J., Lynds, R., Anderson, M.R., (1984) Phys. Rev. B., 30 (2), p. 870; 
Cola{\c c}o, R., Serro, A.P., Eryilmaz, O.L., Erdemir, E., (2009) J. Phys. D Appl. Phys., 42, p. 85307; 
Paul, R., Das, S.N., Dalui, S., Gayen, R.N., Roy, R.K., Bhar, R., Pal, A.K., (2008) J. Phys. D Appl. Phys., 41 (5), p. 55309; 
Serro, A.P., Fernandes, A.C., Saramago, B., (1997) Colloids Surf. A Physicochem. Eng. Aspects, 125, p. 209; 
Serro, A.P., Degiampietro, K., Cola{\c c}o, R., Saramago, B., (2010) Saramago, Adsorption of albumin and sodium hyaluronate on UHMWPE: A QCM-D and AFM study, Colloids and Surfaces B: Biointerfaces, 78, p. 1; 
de Feijter, J.A., Benjamins, J., Veer, F.A., (1978) Biopolymers, 17, p. 1759; 
Gispert, M.P., Serro, A.P., Cola{\c c}o, R., Saramago, B., (2008) Surf. Int. Anal., 40 (12), p. 1529; 
Hook, F., Kasemo, B., Nylander, T., Fant, C., Sott, K., Elwing, H., (2001) Anal. Chem., 73 (24), p. 5796; 
Hook, F., Voros, J., Rodahl, M., Kurrat, R., Boni, P., Ramsden, J.J., Textor, M., Kasemo, B., (2002) Colloids Surf. B Biointerfaces, 24, p. 155; 
Cola{\c c}o, R., Gispert, M.P., Serro, A.P., Saramago, B., (2007) Tribol. Lett., 26 (2), p. 119; 
Gispert, M.P., Serro, A.P., Cola{\c c}o, R., Saramago, B., (2006) Wear, 260, p. 149; 
Serro, A.P., Completo, C., Cola{\c c}o, R., Dos Santos, F., Lobato da Silva, C., Cabral, J.M.S., Ara{\'u}jo, H., Saramago, B., (2009) Surf. Coat. Technol., 203, p. 3701; 
Widmer, M.R., Heuberger, M., V{\"o}r{\"o}s, J., Spencer, N.D., (2001) Tribol. Lett., 10, p. 111; 
Chen, X.M., Jin, Z.M., Fisher, J., (2008) Proc. Inst. Mech. Eng. Part J. Eng. Tribol., 222 (3), p. 513; 
Kumar, P., Oka, M., Ikeuchi, K., Shimizu, K., Yamamuro, T., Okumura, H., Kotoura, Y., (1991) J. Biomater. Mater. Res., 25, p. 813; 
Roba, M., Naka, M., Gautier, E., Spencer, N.D., Crockett, R., (2009) Biomaterials, 30, p. 2072; 
Karuppiah, K.S.K., Sundararajan, S., Xu, Z.-H., Li, X., (2006) Tribol. Lett., 22 (2), p. 181; 
Yang, C.-B., Fang, H.-W., Liu, H.-L., Chang, C.-H., Hsieh, M.-C., Lee, W.-M., Huang, H.-T., (2006) Chem. Phys. Lett., 431, p. 380; 
Fang, H.-W., Wang, H.-C., Tsai, T.-H., Tsai, W.-B., Hou, S.-Y., Liu, H.-L., Lee, W.-H., Huang, C.-H., (2008) J. Appl. Polym. Sci., 108, p. 2428; 
Zappone, B., Rosenberg, K.J., Israelachvili, J., (2007) Tribol. Lett., 26 (3), p. 191; 
Grill, A., (1997) Surf. Coat. Technol., (1-3), p. 507; 
Fontaine, J., Belin, M., Le Mogne, T., Grill, A., (2004) Tribol. Int., 37, p. 869; 
Sanchez-Lopez, J.C., Erdemir, A., Donnet, C., Rojas, T.C., (2003) Surf. Coat. Technol., 163, p. 444; 
Robertson, J., (2002) Mater. Sci. Eng., 37, p. 129
},
	month = {2010///},
	pages = {3451 - 3458},
	abstract = {A commonly used strategy for reduction of wear and friction of the sliding surfaces in prosthetic pairs is the use of hard coatings. DLCs (diamond like carbons) offer a promising solution, because these coatings, already used in other biomedical applications, exhibit a unique combination of chemical inertness, mechanical and tribological properties. In the present work we investigated the role of albumin, the most abundant protein in the periprosthetic fluid, on the tribological behavior of two DLCs with different compositions, against ultra-high molecular weight polyethylene (UHMWPE). The results are compared with those obtained with uncoated stainless steel surfaces. We have characterized the coatings by atomic force microscopy, X-ray diffraction, Raman spectroscopy, ultramicrohardness and wettability measurements. The adsorption of albumin onto the surfaces was investigated by ellipsometry and using a quartz crystal microbalance. Both DLCs adsorb a larger amount of protein than stainless steel. The tribological tests were performed in microtribometer aiming for a deeper understanding of the tribological mechanisms which occur at the submicrometric contact scale. Results showed no significant differences between the tribological behavior of the two coatings. Under the tested conditions, the tribological performance does not improve significantly with the application of the DLCs. Moreover, the presence of albumin on the lubricant leads to higher friction of the polymer against all counterfaces. This effect was attributed to the formation of bridges between the two sliding surfaces by the denaturated protein. The significant differences in the adsorbed amounts of protein onto the stainless steel and the two types of DLCs did not affect considerably the tribological behavior of these materials. {\textcopyright} 2010 Elsevier B.V.},
	keywords = {Adsorbed amount, Adsorption, Albumin adsorption, Atomic force microscopy, Atomic spectroscopy, Biomedical applications, Chemical inertness, Corrosion resistant alloys, Diamond like carbon, DLC coatings, Friction, Hard coatings, Joint prostheses, Mechanical and tribological properties, Mechanical properties, Microtribology, Microtribometer, Nonmetallic matrix composites, Oxide minerals, Polyethylenes, Proteins, Quartz, Raman spectroscopy, Scanning electron microscopy, Sliding surface, Stainless steel, Stainless steel surface, Steel corrosion, Surfaces, Tribological behaviors, Tribological mechanism, Tribological performance, Tribological tests, Tribology, UHMW-PE, UHMWPE, Ultramicrohardness, Wear and friction, Wettability measurements, X ray diffraction},
	isbn = {02578972 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77953715334\&partnerID=40\&md5=d724bda7404d809fed9bafb6a5a337e3},
	author = {Carapeto, A.P. and Serro, A.P. and Nunes, B.M.F. and Martins, M.C.L. and Todorovic, S. and Duarte, M.T. and Andr{\'e}, V. and Cola{\c c}o, R. and Saramago, B.}
}
@article {2244,
	title = {Chitosan-based gene delivery vectors targeted to the peripheral nervous system},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {95},
	year = {2010},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.32874PubMed ID: 20734332Language of Original Document: EnglishCorrespondence Address: P{\^e}go, A. P.; INEB, Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: apego@ineb.up.ptChemicals/CAS: DNA, 9007-49-2; chitosan, 9012-76-4; imidazole, 1467-16-9, 288-32-4; macrogol, 25322-68-3; Cations; Chitosan, 9012-76-4; DNA, 9007-49-2; Polyethylene GlycolsTradenames: Chimarin, medicarb, SwedenManufacturers: medicarb, SwedenReferences: Rodriguez, F.J., Valero-Cabre, A., Navarro, X., Regeneration and functional recovery following peripheral nerve injury (2004) Drug Discov Today, 1, pp. 177-185;Apfel, S.C., Neurotrophic factors in peripheral neuropathies: Therapeutic implications (1999) Brain Pathol, 9, pp. 393-413; 
Apfel, S.C., Is the therapeutic application of neurotrophic factors dead? (2002) Ann Neurol, 51, pp. 8-11; 
Glorioso, J.C., Mata, M., Fink, D.J., Therapeutic gene transfer to the nervous system using viral vectors (2003) J Neurovirol, 9, pp. 165-172; 
Fishman, P.S., Carrigan, D.R., Retrograde transneuronal transfer of the C-fragment of tetanus toxin (1987) Brain Res, 406, pp. 275-279; 
Evinger, C., Erichsen, J.T., Transsynaptic retrograde transport of fragment C of tetanus toxin demonstrated by immunohistochemical localization (1986) Brain Res, 380, pp. 383-388; 
Figueiredo, D.M., Hallewell, R.A., Chen, L.L., Fairweather, N.F., Dougan, G., Savitt, J.M., Parks, D.A., Fishman, P.S., Delivery of recombinant tetanus-superoxide dismutase proteins to central nervous system neurons by retrograde axonal transport (1997) Exp Neurol, 145 (2 PART 1), pp. 546-554; 
Fishman, P.S., Savitt, J.M., Farrand, D.A., Enhanced CNS uptake of systemically administered proteins through conjugation with tetanus C-fragment (1990) J Neurol Sci, 98, pp. 311-325; 
Coen, L., Osta, R., Maury, M., Brulet, P., Construction of hybrid proteins that migrate retrogradely and transynaptically into the central nervous system (1997) Proc Natl Acad Sci USA, 94, pp. 9400-9405; 
Knight, A., Carvajal, J., Schneider, H., Coutelle, C., Chamberlain, S., Fairweather, N., Non-viral neuronal gene delivery mediated by the HC fragment of tetanus toxin (1999) Eur J Biochem, 259, pp. 762-769; 
Oliveira, H., Fernandez, R., Pires, L.R., Sim{\~o}es, S., Martins, M.C.L., Barbosa, M.A., P{\^e}go, A.P., Targeted gene delivery into peripheral sensorial neurons mediated by self-assembled vectors composed of poly(ethilenimine) and tetanus toxin fragment c (2010) J Control Release, 143, pp. 350-358; 
Neu, M., Fischer, D., Kissel, T., Recent advances in rational gene transfer vector design based on poly(ethylene imine) and its derivatives (2005) J Gene Med, 7, pp. 992-1009; 
Tang, G.P., Guo, H.Y., Alexis, F., Wang, X., Zeng, S., Lim, T.M., Ding, J., Wang, S., Low molecular weight polyethylenimines linked by beta-cyclodextrin for gene transfer into the nervous system (2006) J Gene Med, 8, pp. 736-744; 
Chollet, P., Favrot, M.C., Hurbin, A., Coll, J.L., Side-effects of a systemic injection of linear polyethylenimine-DNA complexes (2002) J Gene Med, 4, pp. 84-91; 
Shi, C., Zhu, Y., Ran, X., Wang, M., Su, Y., Cheng, T., Therapeutic potential of chitosan and its derivatives in regenerative medicine (2006) J Surg Res, 133, pp. 185-192; 
Mansouri, S., Lavigne, P., Corsi, K., Benderdour, M., Beaumont, E., Fernandes, J.C., Chitosan-DNA nanoparticles as non-viral vectors in gene therapy: Strategies to improve transfection efficacy (2004) Eur J Pharm Biopharm, 57, pp. 1-8; 
Moreira, C., Oliveira, H., Pires, L.R., Simoes, S., Barbosa, M.A., Pego, A.P., Improving chitosan-mediated gene transfer by the introduction of intracellular buffering moieties into the chitosan backbone (2009) Acta Biomater, 5, pp. 2995-3006; 
Verma, A., Stellacci, F., Effect of surface properties on nanoparticle-cell interactions (2010) Small, 6, pp. 12-21; 
Brugnerotto, J., Lizardi, J., Goycoolea, F.M., Arguelles-Monal, W., Desbrieres, J., Rinaudo, M., An infrared investigation in relation with chitin and chitosan characterization (2001) Polymer, 42, pp. 3569-3580; 
(1987) Guideline on Validation of the Limulus Amebocyte Lysate Test As An End-product Endotoxin Test for Human and Animal Parental Drugs, Biological Products, and Medical Devices, , US Department of Health and Human Services. US Department of Health and Human Services; 
MacUlotti, K., Genta, I., Perugini, P., Imam, M., Bernkop-Schnurch, A., Pavanetto, F., Preparation and in vitro evaluation of thiolated chitosan microparticles (2005) J Microencapsul, 22, pp. 459-470; 
Mao, H., Roy, K., Troung-Le, V.L., Janes, K.A., Lin, K.Y., Wang, Y., August, J.T., Leong, K.W., Chitosan-DNA nanoparticles as gene carriers: Synthesis, characterization and transfection efficiency (2001) J Control Release, 70, pp. 399-421; 
Innes, N.P.T., Ogden, G.R., A technique for the study of endocytosis in human oral epithelial cells (1999) Arch Oral Biol, 44, pp. 519-523; 
O{\textquoteright}Brien, J., Wilson, I., Orton, T., Pognan, F., Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity (2000) Eur J Biochem, 267, pp. 5421-5426; 
Ellman, G.L., Tissue sulfhydryl groups (1959) Arch Biochem Biophys, 82, pp. 70-77; 
Lee, D., Zhang, W., Shirley, S.A., Kong, X., Hellermann, G.R., Lockey, R.F., Mohapatra, S.S., Thiolated chitosan/DNA nanocomplexes exhibit enhanced and sustained gene delivery (2007) Pharm Res, 24, pp. 157-167; 
Loretz, B., Thaler, M., Bernkop-Schnurch, A., Role of sulfhydryl groups in transfection? A case study with chitosan-NAC nanoparticles (2007) Bioconjug Chem, 18, pp. 1028-1035; 
Deinhardt, K., Berninghausen, O., Willison, H.J., Hopkins, C.R., Schiavo, G., Tetanus toxin is internalized by a sequential clathrin-dependent mechanism initiated within lipid microdomains and independent of epsin 1 (2006) J Cell Biol, 174, pp. 459-471; 
Williamson, L.C., Neale, E.A., Bafilomycin A1 inhibits the action of tetanus toxin in spinal-cord neurons in cell-culture (1994) J Neurochem, 63, pp. 2342-2345; 
Bohnert, S., Schiavo, G., Tetanus toxin is transported in a novel neuronal compartment characterized by a specialized pH regulation (2005) J Biol Chem, 280, pp. 42336-42344
},
	month = {2010///},
	pages = {801 - 810},
	abstract = {A non-toxic, targeted, simple and efficient system that can specifically transfect peripheral sensorial neurons can pave the way towards the development of new therapeutics for the treatment of peripheral neuropathies. In this study chitosan (CH), a biodegradable polymer, was used as the starting material in the design of a multicomponent vector targeted to the peripheral nervous system (PNS). Polycation-DNA complexes were optimized using imidazole-and thiol-grafted CH (CHimiSH), in order to increase transfection efficiency and allow the formation of ligand conjugated nanocomplexes, respectively. The 50 kDa non-toxic fragment from the tetanus toxin (HC), shown to interact specifically with peripheral neurons and undergo retrograde transport, was grafted to the binary complex via a bi-functional poly(ethylene glycol) (HC-PEG) reactive for the thiol moieties present in the complex surface. The targeting of the developed nanocomplexes was assessed by means of internalization and transfection studies in the ND7/23 (neuronal) vs. NIH 3T3 (fibroblast) cell lines. Targeted transfection was further confirmed in dorsal root ganglion dissociated primary cultures. A versatile, multi-component nanoparticle system that successfully targets and transfects neuronal cell lines, as well as dorsal root ganglia (DRG) primary neuron cultures was obtained for the 1.0 (w/w) HC-PEG/DNA formulation. {\textcopyright} 2010 Wiley Periodicals, Inc.},
	keywords = {animal cell, Animals, article, Binary complexes, Biodegradable polymers, Carbohydrate Conformation, Carbohydrate Sequence, Cations, Cell culture, Cell lines, cell population, Cells, Cultured, Chitin, chitosan, Complex surface, DNA, DNA complex, Dorsal root ganglia, Efficient systems, embryo, Ethylene, Ethylene Glycol, Female, Ganglia, Spinal, Gene delivery, gene delivery system, Gene delivery vectors, Gene therapy, Gene transfer, Gene Transfer Techniques, genetic transfection, Genetic Vectors, Grafting (chemical), Humans, imidazole, macrogol, materials testing, Mice, Molecular Sequence Data, Molecular Structure, mouse, Multicomponents, Nanocomplexes, Nanoparticle systems, nanoparticles, nerve cell culture, Neuron culture, Neuronal cell, NIH 3T3 Cells, nonhuman, Nontoxic fragment of tetanus toxin, Peripheral nervous system, Peripheral neuropathy, Polycations, Polyethylene Glycols, Polyethylene oxides, Pregnancy, Primary culture, rat, Rats, Rats, Wistar, spinal ganglion, Starting materials, Targeted, Tetanus toxin, Toxic materials, Transfection, Transfection efficiency},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-78249240032\&partnerID=40\&md5=af9c3ca5ca712d53abb5e80f45ed09c0},
	author = {Oliveira, H. and Pires, L.R. and Fernandez, R. and Martins, M.C.L. and Sim{\~o}es, S. and P{\^e}go, A.P.}
}
@article {2250,
	title = {Chitosan/siRNA nanoparticles biofunctionalize nerve implants and enable neurite outgrowth},
	journal = {Nano LettersNano Lett.},
	volume = {10},
	year = {2010},
	note = {Cited By (since 1996): 14Export Date: 21 November 2012Source: Scopusdoi: 10.1021/nl1016909Language of Original Document: EnglishCorrespondence Address: Schlosshauer, B.; NMI Natural and Medical Sciences Institute, University of T{\"u}bingen, Markwiesenstrasse 55, 72770 Reutlingen, Germany; email: schlosshauer@nmi.deChemicals/CAS: chitosan, 9012-76-4; Chitosan, 9012-76-4; RNA, Small Interfering; rhoA GTP-Binding Protein, 3.6.5.2References: Terzis, J.K., Skoulis, T.G., Soucacos, P.N., (1995) Int. Angiol., 14, pp. 264-77;Schmidt, C.E., Leach, J.B., (2003) Annu. Rev. Biomed. Eng., 5, pp. 293-347; 
Stokols, S., Tuszynski, M.H., (2004) Biomaterials, 25 (27), pp. 5839-46; 
Goraltchouk, A., Scanga, V., Morshead, C.M., Shoichet, M.S., (2006) J. Controlled Release, 110 (2), pp. 400-7; 
Wu, J., Chiu, D.T., (1999) Ann. Plast. Surg., 43, pp. 661-667; 
Chen, M.S., Huber, A.B., Van Der Haar, M.E., Frank, M., Schnell, L., Spillmann, A.A., Christ, F., Schwab, M.E., (2000) Nature, 403 (6768), pp. 434-9; 
Grandpre, T., Nakamura, F., Vartanian, T., Strittmatter, S.M., (2000) Nature, 403 (6768), pp. 439-44; 
Liu, B.P., Fournier, A., Grandpre, T., Strittmatter, S.M., (2002) Science, 297 (5584), pp. 1190-3; 
McKerracher, L., David, S., Jackson, D.L., Kottis, V., Dunn, R.J., Braun, P.E., (1994) Neuron, 13 (4), pp. 805-11; 
Mukhopadhyay, G., Doherty, P., Walsh, F.S., Crocker, P.R., Filbin, M.T., (1994) Neuron, 13 (3), pp. 757-67; 
Wang, K.C., Koprivica, V., Kim, J.A., Sivasankaran, R., Guo, Y., Neve, R.L., He, Z., (2002) Nature, 417 (6892), pp. 941-4; 
Mi, S., Lee, X., Shao, Z., Thill, G., Ji, B., Relton, J., Levesque, M., Pepinsky, R.B., (2004) Nat. Neurosci., 7 (3), pp. 221-8; 
Park, J.B., Yiu, G., Kaneko, S., Wang, J., Chang, J., He, X.L., Garcia, K.C., He, Z., (2005) Neuron, 45 (3), pp. 345-51; 
Shao, Z., Browning, J.L., Lee, X., Scott, M.L., Shulga-Morskaya, S., Allaire, N., Thill, G., Mi, S., (2005) Neuron, 45 (3), pp. 353-9; 
Wang, K.C., Kim, J.A., Sivasankaran, R., Segal, R., He, Z., (2002) Nature, 420 (6911), pp. 74-8; 
Wong, S.T., Henley, J.R., Kanning, K.C., Huang, K.H., Bothwell, M., Poo, M.M., (2002) Nat. Neurosci., 5 (12), pp. 1302-8; 
Elbashir, S.M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., Tuschl, T., (2001) Nature, 411 (6836), pp. 494-8; 
Higuchi, H., Yamashita, T., Yoshikawa, H., Tohyama, M., (2003) Biochem. Biophys. Res. Commun., 301 (3), pp. 804-9; 
Fire, A., Xu, S., Montgomery, M.K., Kostas, S.A., Driver, S.E., Mello, C.C., (1998) Nature, 391 (6669), pp. 806-11; 
Tijsterman, M., Plasterk, R.H., (2004) Cell, 117 (1), pp. 1-3; 
De Fougerolles, A., Vornlocher, H.P., Maraganore, J., Lieberman, J., (2007) Nat. Rev. Drug Discovery, 6 (6), pp. 443-53; 
Shrivastava, N., Srivastava, A., (2008) Biotechnol. J., 3 (3), pp. 339-53; 
Soutschek, J., Akinc, A., Bramlage, B., Charisse, K., Constien, R., Donoghue, M., Elbashir, S., Vornlocher, H.P., (2004) Nature, 432 (7014), pp. 173-8; 
Takeshita, F., Ochiya, T., (2006) Cancer Sci., 97 (8), pp. 689-96; 
Bitko, V., Musiyenko, A., Shulyayeva, O., Barik, S., (2005) Nat. Med., 11 (1), pp. 50-5; 
Fjose, A., Drivenes, O., (2006) Birth Defects Res., C, 78 (2), pp. 150-71; 
Whitehead, K.A., Langer, R., Anderson, D.G., (2009) Nat. Rev. Drug Discovery, 8 (2), pp. 129-38; 
Liu, X., Howard, K.A., Dong, M., Andersen, M.O., Rahbek, U.L., Johnsen, M.G., Hansen, O.C., Kjems, J., (2007) Biomaterials, 28 (6), pp. 1280-8; 
Howard, K.A., Rahbek, U.L., Liu, X., Damgaard, C.K., Glud, S.Z., Andersen, M.O., Hovgaard, M.B., Kjems, J., (2006) Mol. Ther., 14 (4), pp. 476-84; 
Sell, S.A., McClure, M.J., Barnes, C.P., Knapp, D.C., Walpoth, B.H., Simpson, D.G., Bowlin, G.L., (2006) Biomed. Mater., 1 (2), pp. 72-80; 
Janes, K.A., Calvo, P., Alonso, M.J., (2001) Adv. Drug Delivery Rev., 47 (1), pp. 83-97; 
Pouton, C.W., Seymour, L.W., (2001) Adv. Drug Delivery Rev., 46 (1-3), pp. 187-203; 
Nel, A.E., Madler, L., Velegol, D., Xia, T., Hoek, E.M., Somasundaran, P., Klaessig, F., Thompson, M., (2009) Nat. Mater., 8 (7), pp. 543-57; 
Ishii, T., Okahata, Y., Sato, T., (2001) Biochim. Biophys. Acta, 1514 (1), pp. 51-64; 
Huang, M., Fong, C.W., Khor, E., Lim, L.Y., (2005) J. Controlled Release, 106 (3), pp. 391-406; 
Richardson, S.C., Kolbe, H.V., Duncan, R., (1999) Int. J. Pharm., 178 (2), pp. 231-43; 
Corsi, K., Chellat, F., Yahia, L., Fernandes, J.C., (2003) Biomaterials, 24 (7), pp. 1255-64; 
Moghimi, S.M., Symonds, P., Murray, J.C., Hunter, A.C., Debska, G., Szewczyk, A., (2005) Mol. Ther., 11 (6), pp. 990-5; 
Koping-Hoggard, M., Tubulekas, I., Guan, H., Edwards, K., Nilsson, M., Varum, K.M., Artursson, P., (2001) Gene Ther., 8 (14), pp. 1108-21; 
Moreira, C., Oliveira, H., Pires, L.R., Simoes, S., Barbosa, M.A., Pego, A.P., (2009) Acta Biomater., 5 (8), pp. 2995-3006; 
Lietz, M., Dreesmann, L., Hoss, M., Oberhoffner, S., Schlosshauer, B., (2006) Biomaterials, 27 (8), pp. 1425-36; 
Ribeiro-Resende, V.T., Koenig, B., Nichterwitz, S., Oberhoffner, S., Schlosshauer, B., (2009) Biomaterials, 30 (29), pp. 5251-9; 
Andersen, M.O., Howard, K.A., Paludan, S.R., Besenbacher, F., Kjems, J., (2008) Biomaterials, 29 (4), pp. 506-12; 
Anchordoquy, T.J., Allison, S.D., Molina, M.C., Girouard, L.G., Carson, T.K., (2001) Drug Discovery Today, 6 (9), pp. 463-470; 
Atala, A., Bauer, S.B., Soker, S., Yoo, J.J., Retik, A.B., (2006) Lancet, 367 (9518), pp. 1241-6; 
Lin, C.Y., Schek, R.M., Mistry, A.S., Shi, X., Mikos, A.G., Krebsbach, P.H., Hollister, S.J., (2005) Tissue Eng., 11 (9-10), pp. 1589-98; 
Suggate, E.L., Ahmed, Z., Read, M.L., Eaton-Charnock, K., Douglas, M.R., Gonzalez, A.M., Berry, M., Logan, A., (2009) Mol. Cell Neurosci., 40 (4), pp. 451-62; 
Ahmed, Z., Dent, R.G., Suggate, E.L., Barrett, L.B., Seabright, R.J., Berry, M., Logan, A., (2005) Mol. Cell Neurosci., 28 (3), pp. 509-23; 
Fournier, A.E., Takizawa, B.T., Strittmatter, S.M., (2003) J. Neurosci., 23 (4), pp. 1416-23
},
	month = {2010///},
	pages = {3933 - 3939},
	abstract = {Microstructured 20 μm thick polymer filaments used as nerve implants were loaded with chitosan/siRNA nanoparticles to promote nerve regeneration and ensure local delivery of nanotherapeutics. The stable nanoparticles were rapidly internalized by cells and did not affect cell viability. Target mRNA was successfully reduced by 65-75\% and neurite outgrowth was enhanced even in an inhibitory environment. This work, thus, supports the application of nanobiofunctionalized implants as a novel approach for spinal cord and nerve repair. {\textcopyright} 2010 American Chemical Society.},
	keywords = {animal, Animals, article, Cell Line, cell viability, chemistry, Chitin, chitosan, cytology, Drug delivery, genetic transfection, genetics, Local delivery, metabolism, Microstructured, nanobiofunctionalized implants, nanoparticle, nanoparticles, nerve cell, nerve regeneration, Nerve repair, neurite, Neurite outgrowth, Neurites, Neurons, Polymer filaments, Prostheses and Implants, prostheses and orthoses, rhoA GTP-Binding Protein, RhoA guanine nucleotide binding protein, RNA, RNA, Small Interfering, siRNA, small interfering RNA, Spinal cords, Transfection, ultrastructure},
	isbn = {15306984 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77958033530\&partnerID=40\&md5=6862e82e01c3aedf5423ebb14248c6ec},
	author = {Mittnacht, U. and Hartmann, H. and Hein, S. and Oliveira, H. and Dong, M. and P{\^e}go, A.P. and Kjems, J. and Howard, K.A. and Schlosshauer, B.}
}
@article {2283,
	title = {Comparison of a computer system evaluation of intrapartum cardiotocographic events and a consensus of clinicians.},
	journal = {Journal of perinatal medicineJ Perinat Med},
	volume = {38},
	year = {2010},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: Scopusdoi: 10.1515/JPM.2010.030PubMed ID: 20121542Language of Original Document: EnglishCorrespondence Address: Costa, M.A.email: cosantonia@gmail.com},
	month = {2010///},
	pages = {191 - 195},
	abstract = {AIMS: To compare between computer analysis of intrapartum cardiotocography (CTG) features by the Omniview-SisPorto 3.5 and a consensus of clinicians. METHODS: Agreement study using 50 consecutively acquired tracings (206 h of signals) with >60 min duration, <10\% signal loss and recorded in labor at term by internal fetal heart rate (FHR) monitoring. Tracings were divided into 10-min segments and independently analyzed by three experienced clinicians, in order to estimate the FHR baseline and identify periodic events. A consensus was reached using a three round Delphi procedure. Results were compared with the analysis provided by the Omniview-SisPorto 3.5 system. RESULTS: For baseline estimation, agreement between the computer and the consensus was high [intraclass correlation coefficient (ICC)=0.85; 95\% confidence interval (CI) 0.46-0.93], with a mean difference of 3.7 bpm (limits of agreement -4.4-11.9 bpm), and 99\% of differences under 15 bpm. A concordant identification was observed in 71\% of accelerations (95\% CI: 69\%-73\%), 68\% of decelerations (95\% CI: 66\%-70\%), and 87\% of uterine contractions (95\% CI: 85\%-89\%). CONCLUSIONS: A high agreement was observed between the Omniview-SisPorto 3.5 and a consensus of clinicians in evaluation of intrapartum CTG baseline, accelerations, decelerations and uterine contractions.},
	keywords = {algorithm, Algorithms, article, Cardiotocography, comparative study, computer assisted diagnosis, Diagnosis, Computer-Assisted, Female, Fetal Heart, Fetal monitoring, fetus heart, fetus monitoring, human, Humans, instrumentation, methodology, physician, Physicians, physiology, Pregnancy},
	isbn = {16193997 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-77953038696\&partnerID=40\&md5=bca187de4ffea0b2f2c4d2dcfb2140d9},
	author = {Costa, M.A. and Ayres-de-Campos, D. and Machado, A.P. and Santos, C.C. and Bernardes, J.}
}
@article {2322,
	title = {Cancer cell angiogenic capability is regulated by 3D culture and integrin engagement},
	journal = {Proceedings of the National Academy of Sciences of the United States of AmericaProc. Natl. Acad. Sci. U. S. A.},
	volume = {106},
	year = {2009},
	note = {Cited By (since 1996): 48Export Date: 21 November 2012Source: ScopusCODEN: PNASAdoi: 10.1073/pnas.0808932106PubMed ID: 19126683Language of Original Document: EnglishCorrespondence Address: Mooney, D. J.; School of Engineering and Applied Sciences, Harvard University, 40 Oxford Street, Cambridge, MA 02138, United States; email: mooneyd@seas.harvard.eduChemicals/CAS: arginylglycylaspartic acid, 99896-85-2; interleukin 8, 114308-91-7; vasculotropin, 127464-60-2; Hydrogels; Integrins; Interleukin-8; Vascular Endothelial Growth Factor AReferences: Debnath, J., Brugge, J.S., Modelling glandular epithelial cancers in 3-dimensional cultures (2005) Nat Rev Cancer, 5, pp. 675-688;Wang, F., Reciprocal interactions between β1-integrin and epidermal growth factor receptor in 3-dimensional basement membrane breast cultures: A different perspective in epithelial biology (1998) Proc Natl Acad Sci USA, 95, pp. 14821-14826; 
Fischbach, C., Engineering tumors with 3D scaffolds (2007) Nat Methods, 4, pp. 855-860; 
Paszek, M.J., Tensional homeostasis and the malignant phenotype (2005) Cancer Cell, 8, pp. 241-254; 
Hanahan, D., Weinberg, R.A., The hallmarks of cancer (2000) Cell, 100, pp. 57-70; 
De, S., VEGF-integrin interplay controls tumor growth and vascularization (2005) Proc Natl Acad Sci USA, 102, pp. 7589-7594; 
Lowrie, A.G., Salter, D.M., Ross, J.A., Latent effects of fibronectin, α5β1 integrin, αvβ5 integrin, and the cytoskeleton regulate pancreatic carcinoma cell IL-8 secretion (2004) Br J Cancer, 91, pp. 1327-1334; 
Cukierman, E., Pankov, R., Stevens, D.R., Yamada, K.M., Taking cell matrix adhesions to the third dimension (2001) Science, 294, pp. 1708-1712; 
Rowley, J.A., Madlambayan, G., Mooney, D.J., Alginate hydrogels as synthetic extracellular matrix materials (1999) Biomaterials, 20, pp. 45-53; 
Lingen, M.W., Angiogenesis in the development of head and neck cancer and its inhibition by chemopreventive agents (1999) Crit Rev Oral Biol Med, 10, pp. 153-164; 
Fischbach, C., Mooney, D., Polymeric systems for bioinspired delivery of angiogenic molecules (2006) Adv Polym Sci, 203, pp. 191-221; 
Ruoslahti, E., Pierschbacher, M.D., New perspectives in cell adhesion: RGD and integrins (1987) Science, 238, pp. 491-497; 
Kong, H.J., Boontheekul, T., Mooney, D.J., Quantifying the relation between adhesion ligand-receptor bond formation and cell phenotype (2006) Proc Natl Acad Sci USA, 103, pp. 18534-18539; 
Harris, A.L., Hypoxia: A key regulatory factor in tumour growth (2002) Nat Rev Cancer, 2, pp. 38-47; 
Xu, L., Hypoxia-induced elevation in interleukin-8 expression by human ovarian carcinoma cells (1999) Cancer Res, 59, pp. 5822-5829; 
Weaver, V.M., Reversion of the malignant phenotype of human breast cells in three-dimensional cultureandin vivo by integrin-blocking antibodies (1997) J Cell Biol, 137, pp. 231-245; 
Chung, J., Hypoxia-induced vascular endothelial growth factor transcription and protection from apoptosis are dependent on α6β1 integrin in breast carcinoma cells (2004) Cancer Res, 64, pp. 4711-4716; 
Ghosh, S., Three-dimensional culture of melanoma cells profoundly affects gene expression profile: A high density oligonucleotide array study (2005) J Cell Physiol, 204, pp. 522-531; 
De Larco, J.E., A potential role for interleukin-8 in the metastatic phenotype of breast carcinoma cells (2001) Am J Pathol, 158, pp. 639-646; 
Nemeth, Z.H., Disruption of the actin cytoskeleton results in nuclear factor-κB activation and inflammatory mediator production in cultured human intestinal epithelial cells (2004) J Cell Physiol, 200, pp. 71-81; 
Xie, K., Interleukin-8 and human cancer biology (2001) Cytokine Growth Factor Rev, 12, pp. 375-391; 
Casanovas, O., Hicklin, D.J., Bergers, G., Hanahan, D., Drug resistance by evasion of antiangiogenic targeting of VEGF signaling in late-stage pancreatic islet tumors (2005) Cancer Cell, 8, pp. 299-309; 
Mizukami, Y., Induction of interleukin-8 preserves the angiogenic response in HIF-1α-deficient colon cancer cells (2005) Nat Med, 11, pp. 992-997; 
Esposito, I., Inflammatory cells contribute to the generation of an angiogenic phenotype in pancreatic ductal adenocarcinoma (2004) J Clin Pathol, 57, pp. 630-636; 
Ferrara, N., Henzel, W.J., Pituitary follicular cells secrete a novel heparin-binding growth factor specific for vascular endothelial cells (1989) Biochem Biophys Res Commun, 161, pp. 851-858; 
Gaertner, H.F., Offord, R.E., Site-specific attachment of functionalized poly(ethylene glycol) to the amino terminus of proteins (1996) Bioconjug Chem, 7, pp. 38-44; 
Goerges, A.L., Nugent, M.A., Regulation of vascular endothelial growth factor binding and activity by extracellular pH (2003) J Biol Chem, 278, pp. 19518-19525; 
Kuschert, G.S., Identification of a glycosaminoglycan binding surface on human interleukin-8 (1998) Biochemistry, 37, pp. 11193-11201; 
Kerbel, R., Folkman, J., Clinical translation of angiogenesis inhibitors (2002) Nat Rev Cancer, 2, pp. 727-739; 
Fischbach, C., Mooney, D.J., Polymers for pro- and antiangiogenic therapy (2007) Biomaterials, 28, pp. 2069-2076; 
Alsberg, E., Engineering growing tissues (2002) Proc Natl Acad Sci USA, 99, pp. 12025-12030; 
Simmons, C.A., Dual growth factor delivery and controlled scaffold degradation enhance in vivo bone formation by transplanted bone marrow stromal cells (2004) Bone, 35, pp. 562-569; 
Comisar, W.A., Kazmers, N.H., Mooney, D.J., Linderman, J.J., Engineering RGD nanopatterned hydrogels to control preosteoblast behavior: A combined computational and experimental approach (2007) Biomaterials, 28, pp. 4409-4417; 
Alsberg, E., Regulating bone formation via controlled scaffold degradation (2003) J Dent Res, 82, pp. 903-908; 
Richardson, T.P., Peters, M.C., Ennett, A.B., Mooney, D.J., Polymeric system for dual growth factor delivery (2001) Nat Biotechnol, 19, pp. 1029-1034
},
	month = {2009///},
	pages = {399 - 404},
	abstract = {Three-dimensional culture alters cancer cell signaling; however, the underlying mechanisms and importance of these changes on tumor vascularization remain unclear. A hydrogel system was used to examine the role of the transition from 2D to 3D culture, with and without integrin engagement, on cancer cell angiogenic capability. Three-dimensional culture recreated tumor microenvironmental cues and led to enhanced interleukin 8 (IL-8) secretion that depended on integrin engagement with adhesion peptides coupled to the polymer. In contrast, vascular endothelial growth factor (VEGF) secretion was unaffected by 3D culture with or without substrate adhesion. IL-8 diffused greater distances and was present in higher concentrations in the systemic circulation, relative to VEGF. Implantation of a polymeric IL-8 delivery system into GFP bone marrow-transplanted mice revealed that localized IL-8 up-regulation was critical to both the local and systemic control of tumor vascularization in vivo. In summary, 3D integrin engagement within tumor microenvironments regulates cancer cell angiogenic signaling, and controlled local and systemic blockade of both IL-8 and VEGF signaling may improve antiangiogenic therapies. {\textcopyright} 2009 by The National Academy of Sciences of the USA.},
	keywords = {animal experiment, animal model, Animals, arginylglycylaspartic acid, article, bone marrow transplantation, cancer cell, cancer cell culture, Cell Culture Techniques, concentration (parameters), controlled study, cytokine release, Diffusion, Drug delivery, ECM, Humans, hydrogel, Hydrogels, IL-8, implantation, in vivo study, integrin, Integrins, interleukin 8, Interleukin-8, Mice, microenvironment, Models, Biological, mouse, Mus, Neoplasms, Neovascularization, Pathologic, nonhuman, polymer, priority journal, signal transduction, systemic circulation, Tumor vascularization, upregulation, Vascular Endothelial Growth Factor A, vasculotropin},
	isbn = {00278424 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-58849121351\&partnerID=40\&md5=acc07042d6f5801f7b704a3f355917cf},
	author = {Fischbach, C. and Hyun, J.K. and Hsiong, S.X. and Evangelista, M.B. and Yuen, W. and Mooney, D.J.}
}
@article {2307,
	title = {Cancer invasion and metastasis: Interacting ecosystems},
	journal = {Virchows ArchivVirchows Arch.},
	volume = {454},
	year = {2009},
	note = {Cited By (since 1996): 20Export Date: 21 November 2012Source: ScopusCODEN: VARCEdoi: 10.1007/s00428-009-0784-0PubMed ID: 19471961Language of Original Document: EnglishCorrespondence Address: Madani, I.; Department of Radiotherapy, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, Belgium; email: indira.madani@ugent.beReferences: Tarin, D., New insights into the pathogenesis of breast cancer metastasis (2006) Breast Disease, 26 (1), pp. 13-25;Stafford, L.J., Vaidya, K.S., Welch, D.R., Metastasis suppressors genes in cancer (2008) Int J Biochem Cell Biol, 40, pp. 874-891; 
Oliveira, M.J., (2004) The Effect of Bacteria on Colon Cancer Cell Invasion: Molecular Mechanisms Associated, , Faculty of Medicine and Health Sciences. Ghent University. PhD thesis; 
Madani, I., De Neve, W., Mareel, M., Does ionizing radiation stimulate cancer invasion and metastasis? (2008) Bull Cancer, 95, pp. 292-300; 
De Wever, O., Mareel, M., Role of tissue stroma in cancer cell invasion (2003) Journal of Pathology, 200 (4), pp. 429-447. , DOI 10.1002/path.1398; 
Bracke, M.E., Vanhoecke, B.W.A., Derycke, L., Bolca, S., Possemiers, S., Heyerick, A., Stevens, C.V., Parmar, V.S., Plant polyphenolics as anti-invasive cancer agents (2008) Anti-Cancer Agents in Medicinal Chemistry, 8 (2), pp. 171-185. , http://www.ingentaconnect.com/content/ben/acamc/2008/00000008/00000002/ art00004, DOI 10.2174/187152008783497037; 
Ottewell, P.D., Coleman, R.E., Holen, I., From genetic abnormality to metastases: Murine models of breast cancer and their use in the development of anticancer therapies (2006) Breast Cancer Res Treat, 96, pp. 101-113; 
Langley, R.R., Fidler, I.J., Tumor cell-organ microenvironment interactions in the pathogenesis of cancer metastasis (2007) Endocrine Reviews, 28 (3), pp. 297-321. , http://edrv.endojournals.org/cgi/reprint/28/3/297.pdf, DOI 10.1210/er.2006-0027; 
Kuperwasser, C., Chavarria, T., Wu, M., Magrane, G., Gray, J.W., Carey, L., Richardson, A., Weinberg, R.A., Reconstruction of functionally normal and malignant human breast tissues in mice (2004) Proceedings of the National Academy of Sciences of the United States of America, 101 (14), pp. 4966-4971. , DOI 10.1073/pnas.0401064101; 
Hoffman, R.M., Real-time subcellular imaging in live animals: New visible targets for cancer drug discovery (2006) IDrugs, 9 (9), pp. 632-635; 
Fomchenko, E.I., Holland, E.C., Mouse models of brain tumors and their applications in preclinical trials (2006) Clinical Cancer Research, 12 (18), pp. 5288-5297. , DOI 10.1158/1078-0432.CCR-06-0438; 
Gingrich, J.R., Barrios, R.J., Morton, R.A., Boyce, B.F., DeMayo, F.J., Finegold, M.J., Angelopoulou, R., Greenberg, N.M., Metastatic prostate cancer in a transgenic mouse (1996) Cancer Research, 56 (18), pp. 4096-4102; 
Peri, A.-K., Wilgenbus, P., Dahl, U., Semb, H., Christofori, G., A causal role for E-cadherin in the transition from adenoma to carcinoma (1998) Nature, 392 (6672), pp. 190-193. , DOI 10.1038/32433; 
Ambartsumian, N., Grigorian, M., Lukanidin, E., Genetically modified mouse models to study the role of metastasis-promoting S100A4(mts1) protein in metastatic mammary cancer (2005) Journal of Dairy Research, 72 (SPEC. ISS.), pp. 27-33. , DOI 10.1017/S0022029905001093; 
Scherer, R.L., McIntyre, J.O., Matrisian, L.M., Imaging matrix metalloproteinases in cancer (2008) Cancer Metastasis Rev, 27, pp. 679-690; 
Espina, V., Heiby, M., Pierobon, M., Liotta, L.A., Laser capture microdissection technology (2007) Expert Review of Molecular Diagnostics, 7 (5), pp. 647-657. , http://www.future-drugs.com/doi/pdf/10.1586/14737159.7.5.647, DOI 10.1586/14737159.7.5.647; 
Eccles, S.A., Welch, D.R., Metastasis: recent discoveries and novel treatment strategies (2007) Lancet, 369 (9574), pp. 1742-1757. , DOI 10.1016/S0140-6736(07)60781-8, PII S0140673607607818; 
Brenton, J.D., Carey, L.A., Ahmed, A., Caldas, C., Molecular classification and molecular forecasting of breast cancer: Ready for clinical application? (2005) Journal of Clinical Oncology, 23 (29), pp. 7350-7360. , DOI 10.1200/JCO.2005.03.3845; 
Montel, V., Huang, T.-Y., Mose, E., Pestonjamasp, K., Tarin, D., Expression profiling of primary tumors and matched lymphatic and lung metastases in a xenogeneic breast cancer model (2005) American Journal of Pathology, 166 (5), pp. 1565-1579; 
Karpf, A.R., Jones, D.A., Reactivating the expression of methylation silenced genes in human cancer (2002) Oncogene, 21 (35 REV. ISS. 3), pp. 5496-5503. , DOI 10.1038/sj.onc.1205602; 
Feng, Y., Sun, B., Li, X., Zhang, L., Niu, Y., Xiao, C., Ning, L., Hao, X., Differentially expressed genes between primary cancer and paired lymph node metastases predict clinical outcome of node-positive breast cancer patients (2007) Breast Cancer Research and Treatment, 103 (3), pp. 319-329. , DOI 10.1007/s10549-006-9385-7; 
Van{\textquoteright}t Veer, L.J., Dai, H., Van De Vijver, M.J., He, Y.D., Hart, A.A.M., Mao, M., Peterse, H.L., Friend, S.H., Gene expression profiling predicts clinical outcome of breast cancer (2002) Nature, 415 (6871), pp. 530-536. , DOI 10.1038/415530a; 
Wang, W., Klijn, J.G., Zhang, Y., Gene-expression profiles to predict distant metastasis of lymph-node-negative primary breast cancer (2005) Lancet, 415, pp. 530-536; 
Eckhardt, B.L., Parker, B.S., Van Laar, R.K., Restall, C.M., Natoli, A.L., Tavaria, M.D., Stanley, K.L., Anderson, R.L., Genomic analysis of a spontaneous model of breast cancer metastasis to bone reveals a role for the extracellular matrix (2005) Molecular Cancer Research, 3 (1), pp. 1-13; 
Adib, T.R., Henderson, S., Perrett, C., Hewitt, D., Bourmpoulia, D., Ledermann, J., Boshoff, C., Predicting biomarkers for ovarian cancer using gene-expression microarrays (2004) British Journal of Cancer, 90 (3), pp. 686-692. , DOI 10.1038/sj.bjc.6601603; 
Suzuki, M., Tarin, D., Gene expression profiling of human lymph node metastases and matched primary breast carcinomas: Clinical implications (2007) Molecular Oncology, 1 (2), pp. 172-180. , DOI 10.1016/j.molonc.2007.03.005, PII S1574789107000154; 
Chang, H.Y., Nuyten, D.S.A., Sneddon, J.B., Hastie, T., Tibshirani, R., Sorlie, T., Dai, H., Van De Vijver, M.J., Robustness, scalability, and integration of a wound-response gene expression signature in predicting breast cancer survival (2005) Proceedings of the National Academy of Sciences of the United States of America, 102 (10), pp. 3738-3743. , DOI 10.1073/pnas.0409462102; 
Finak, G., Bertos, N., Pepin, F., Sadekova, S., Souleimanova, M., Zhao, H., Chen, H., Park, M., Stromal gene expression predicts clinical outcome in breast cancer (2008) Nature Medicine, 14 (5), pp. 518-527. , DOI 10.1038/nm1764, PII NM1764; 
Clark, I.M., Swingler, T.E., Sampieri, C.L., The regulation of matrix metalloproteinases and their inhibitors (2008) Int J Biochem Cell Biol, 40, pp. 1362-1378; 
Li, Y., Liang, J., Kang, S., Dong, Z., Wang, N., Xing, H., Zhou, R., Zhao, X., E-cadherin gene polymorphisms and haplotype associated with the occurrence of epithelial ovarian cancer in Chinese (2008) Gynecologic Oncology, 108 (2), pp. 409-414. , DOI 10.1016/j.ygyno.2007.10.024, PII S0090825807008517; 
Brendle, A., Lei, H., Brandt, A., Polymorphisms in predicted microRNA-binding sites in integrin genes and breast cancer: ITGB4 as prognostic marker (2008) Carcinogenesis, 29, pp. 1394-1399; 
Hunter, K.W., Crawford, N.P., Germ line polymorphism in metastatic progression (2006) Cancer Research, 66 (3), pp. 1251-1254. , DOI 10.1158/0008-5472.CAN-05-3705; 
Crawford, N.P., Ziogas, A., Peel, D.J., Germline polymorphisms in SIPA1 are associated with metastasis and other indicators of poor prognosis in breast cancer (2006) Breast Cancer Res, 8, p. 16; 
Van Roy, F., Berx, G., The cell-cell adhesion molecule E-cadherin (2008) Cell Mol Life Sci, 65, pp. 3756-3788; 
Bartel, D.P., Chen, C.-Z., Micromanagers of gene expression: The potentially widespread influence of metazoan microRNAs (2004) Nature Reviews Genetics, 5 (5), pp. 396-400; 
Ma, L., Weinberg, R.A., Micromanagers of malignancy: Role of microRNAs in regulating metastasis (2008) Trends Genet, 24, pp. 448-456; 
Graff, J.R., Zimmer, S.G., Translational control and metastatic progression: Enhanced activity of the mRNA cap-binding protein eIF-4E selectively enhances translation of metastasis-related mRNAs (2003) Clinical and Experimental Metastasis, 20 (3), pp. 265-273. , DOI 10.1023/A:1022943419011; 
Hao, X., Sun, B., Hu, L., Lahdesmaki, H., Dunmire, V., Feng, Y., Zhang, S.-W., Zhang, W., Differential Gene and Protein Expression in Primary Breast Malignancies and Their Lymph Node Metastases as Revealed by Combined cDNA Microarray and Tissue Microarray Analysis (2004) Cancer, 100 (6), pp. 1110-1122. , DOI 10.1002/cncr.20095; 
Hakomori, S.-I., Tumor malignancy defined by aberrant glycosylation and sphingo(glyco)lipid metabolism (1996) Cancer Research, 56 (23), pp. 5309-5318; 
Yoshimura, M., Ihara, Y., Matsuzawa, Y., Taniguchi, N., Aberrant glycosylation of E-cadherin enhances cell-cell binding to suppress metastasis (1996) Journal of Biological Chemistry, 271 (23), pp. 13811-13815. , DOI 10.1074/jbc.271.23.13811; 
McLachlan, R.W., Yap, A.S., Not so simple: The complexity of phosphotyrosine signaling at cadherin adhesive contacts (2007) Journal of Molecular Medicine, 85 (6), pp. 545-554. , DOI 10.1007/s00109-007-0198-x, Special Topic: Tumor Cell Invasion and Metastasis; 
Wipff, P.J., Hinz, B., Integrins and the activation of latent transforming growth factor beta1-an intimate relationship (2008) Eur J Cell Biol, 87, pp. 601-615; 
Paredes, J., Correia, A.L., Ribeiro, A.S., P-cadherin expression in breast cancer: A review (2007) Breast Cancer Res, 9, p. 214; 
Van Marck, V., Stove, C., Van Den Bossche, K., Stove, V., Paredes, J., Vander Haeghen, Y., Bracke, M., P-cadherin promotes cell-cell adhesion and counteracts invasion in human melanoma (2005) Cancer Research, 65 (19), pp. 8774-8783. , DOI 10.1158/0008-5472.CAN-04-4414; 
Wheelock, M.J., Shintani, Y., Maeda, M., Fukumoto, Y., Johnson, K.R., Cadherin switching (2008) Journal of Cell Science, 121 (6), pp. 727-735. , DOI 10.1242/jcs.000455; 
De Wever, O., Westbroek, W., Verloes, A., Bloemen, N., Bracke, M., Gespach, C., Bruyneel, E., Mareel, M., Critical role of N-cadherin in myofibroblast invasion and migration in vitro stimulated by colon-cancer-cell-derived TGF-β or wounding (2004) Journal of Cell Science, 117 (20), pp. 4691-4703. , DOI 10.1242/jcs.01322; 
Alexander, N.R., Tran, N.L., Rekapally, H., N-cadherin gene expression in prostate carcinoma is modulated by integrin-dependent nuclear translocation of Twist1 (2006) Cancer Res, 66, pp. 3365-3369; 
Stove, C., Bracke, M., Roles for neuregulins in human cancer (2005) Clinical and Experimental Metastasis, 21 (8), pp. 665-684. , DOI 10.1007/s10585-004-6917-6; 
Kerbel, R.S., Tumor angiogenesis (2008) New England Journal of Medicine, 358 (19), pp. 2039-2049. , http://content.nejm.org/cgi/reprint/358/19/2039.pdf, DOI 10.1056/NEJMra0706596; 
Chabottaux, V., Noel, A., Breast cancer progression: Insights into multifaceted matrix metalloproteinases (2007) Clinical and Experimental Metastasis, 24 (8), pp. 647-656. , DOI 10.1007/s10585-007-9113-7; 
Noel, A., Jost, M., Maquoi, E., Matrix metalloproteinases at cancer tumor-host interface (2008) Seminars in Cell and Developmental Biology, 19 (1), pp. 52-60. , DOI 10.1016/j.semcdb.2007.05.011, PII S1084952107000821, Matrix Metalloproteinases; 
Jones, R.B., Gordus, A., Krall, J.A., MacBeath, G., A quantitative protein interaction network for the ErbB receptors using protein microarrays (2006) Nature, 439 (7073), pp. 168-174. , DOI 10.1038/nature04177, PII NATURE04177; 
Pawson, T., Nash, P., Assembly of cell regulatory systems through protein interaction domains (2003) Science, 300 (5618), pp. 445-452. , DOI 10.1126/science.1083653; 
Kopfstein, L., Christofori, G., Metastasis: Cell-autonomous mechanisms versus contributions by the tumor microenvironment (2006) Cell Mol Life Sci, 63, pp. 449-468; 
Paget, S., The distribution of secondary growths in cancer of the breast (1889) Lancet, 1, pp. 571-573; 
Mareel, M.M., Behrens, J., Birchmeier, W., Down-regulation of E-cadherin expression in Madin Darby canine kidney (MDCK) cells inside tumors of nude mice (1991) Int J Cancer, 47, pp. 922-928; 
Creighton, C.J., Bromberg-White, J.L., Misek, D.E., Monsma, D.J., Brichory, F., Kuick, R., Giordano, T.J., Hanash, S.M., Analysis of tumor-host interactions by gene expression profiling of lung adenocarcinoma xenografts identifies genes involved in tumor formation (2005) Molecular Cancer Research, 3 (3), pp. 119-129. , DOI 10.1158/1541-7786.MCR-04-0189; 
Dimanche-Boitrel, M.T., Vakaet Jr., L., Pujuguet, P., Chauffert, B., Martin, M.S., Hammann, A., Van Roy, F., Martin, F., In vivo and in vitro invasiveness of a rat colon-cancer cell line maintaining E-cadherin expression: An enhancing role of tumor-associated myofibroblasts (1994) International Journal of Cancer, 56 (4), pp. 512-521; 
Lin, E.Y., Pollard, J.W., Tumor-associated macrophages press the angiogenic switch in breast cancer (2007) Cancer Research, 67 (11), pp. 5064-5066. , http://cancerres.aacrjournals.org/cgi/reprint/67/11/5064, DOI 10.1158/0008-5472.CAN-07-0912; 
Korc, M., Pancreatic cancer-associated stroma production (2007) Am J sur, 194 (4 SUPPL.), pp. 84-S86; 
He, Y., Rajantie, I., Pajusola, K., Jeltsch, M., Holopainen, T., Yla-Herttuala, S., Harding, T., Alitalo, K., Vascular endothelial cell growth factor receptor 3-mediated activation of lymphatic endothelium is crucial for tumor cell entry and spread via lymphatic vessels (2005) Cancer Research, 65 (11), pp. 4739-4746. , DOI 10.1158/0008-5472.CAN-04-4576; 
Mahabeleshwar, G.H., Byzova, T.V., Angiogenesis in Melanoma (2007) Seminars in Oncology, 34 (6), pp. 555-565. , DOI 10.1053/j.seminoncol.2007.09.009, PII S0093775407001832, Malignant Melanoma; 
Benoy, I.H., Salgado, R., Elst, H., Relative microvessel area of the primary tumour, and not lymph node status, predicts the presence of bone marrow micrometastases detected by reverse transcriptase polymerase chain reaction in patients with clinically non-metastatic breast cancer (2005) Breast Cancer Res, 7, pp. 210-R219; 
Hicklin, D.J., Ellis, L.M., Role of the vascular endothelial growth factor pathway in tumor growth and angiogenesis (2005) Journal of Clinical Oncology, 23 (5), pp. 1011-1027. , DOI 10.1200/JCO.2005.06.081; 
Schoppmann, S.F., Bayer, G., Aumayr, K., Taucher, S., Geleff, S., Rudas, M., Kubista, E., Horvat, R., Prognostic value of lymphangiogenesis and lymphovascular invasion in invasive breast cancer (2004) Annals of Surgery, 240 (2), pp. 306-312. , DOI 10.1097/01.sla.0000133355.48672.22; 
Tobler, N.E., Detmar, M., Tumor and lymph node lymphangiogenesis - Impact on cancer metastasis (2006) Journal of Leukocyte Biology, 80 (4), pp. 691-696. , http://www.jleukbio.org/cgi/reprint/80/4/691, DOI 10.1189/jlb.1105653; 
Nathanson, S.D., Leong, S., Preclinical models of regional lymph node tumor metastasis (2007) Cancer Metastasis and the Lymphvascular System: Basis for Rational Therapy, pp. 129-156. , Springer New York; 
Wissmann, C., Detmar, M., Pathways targeting tumor lymphangiogenesis (2006) Clinical Cancer Research, 12 (23), pp. 6865-6868. , DOI 10.1158/1078-0432.CCR-06-1800; 
Rafii, D.C., Psaila, B., Butler, J., Jin, D.K., Lyden, D., Regulation of vasculogenesis by platelet-mediated recruitment of bone marrow-derived cells (2008) Arteriosclerosis, Thrombosis, and Vascular Biology, 28 (2), pp. 217-222. , DOI 10.1161/ATVBAHA.107.151159, PII 0004360520080200000004; 
Mantovani, A., Sica, A., Sozzani, S., Allavena, P., Vecchi, A., Locati, M., The chemokine system in diverse forms of macrophage activation and polarization (2004) Trends in Immunology, 25 (12), pp. 677-686. , DOI 10.1016/j.it.2004.09.015, PII S1471490604002959; 
Mantovani, A., Romero, P., Palucka, A.K., Tumour immunity: Effector response to tumour and role of the microenvironment (2008) Lancet, 371, pp. 771-783; 
Talmadge, J.E., Donkor, M., Scholar, E., Inflammatory cell infiltration of tumors: Jekyll or Hyde (2007) Cancer and Metastasis Reviews, 26 (3-4), pp. 373-400. , DOI 10.1007/s10555-007-9072-0, Forty Years of Metastasis Research: A Tribute to Dr. Isaiah J. Fidler; 
Lewis, C.E., Pollard, J.W., Distinct role of macrophages in different tumor microenvironments (2006) Cancer Research, 66 (2), pp. 605-612. , DOI 10.1158/0008-5472.CAN-05-4005; 
Condeelis, J., Pollard, J.W., Macrophages: Obligate partners for tumor cell migration, invasion, and metastasis (2006) Cell, 124 (2), pp. 263-266. , DOI 10.1016/j.cell.2006.01.007, PII S0092867406000559; 
Wyckoff, J.B., Wang, Y., Lin, E.Y., Li, J.-F., Goswami, S., Stanley, E.R., Segall, J.E., Condeelis, J., Direct visualization of macrophage-assisted tumor cell intravasation in mammary tumors (2007) Cancer Research, 67 (6), pp. 2649-2656. , DOI 10.1158/0008-5472.CAN-06-1823; 
Mahadevan, D., Von Hoff, D.D., Tumor-stroma interactions in pancreatic ductal adenocarcinoma (2007) Molecular Cancer Therapeutics, 6 (4), pp. 1186-1197. , DOI 10.1158/1535-7163.MCT-06-0686; 
De Wever, O., Demetter, P., Mareel, M., Stromal myofibroblasts are drivers of invasive cancer growth (2008) Int J Cancer, 123, pp. 2229-2238; 
Massagu{\`e}, J., TGFbeta in cancer (2008) Cell, 134, pp. 215-230; 
Hu, M., Yao, J., Carroll, D.K., Weremowicz, S., Chen, H., Carrasco, D., Richardson, A., Polyak, K., Regulation of In Situ to Invasive Breast Carcinoma Transition (2008) Cancer Cell, 13 (5), pp. 394-406. , DOI 10.1016/j.ccr.2008.03.007, PII S1535610808000913; 
Lerwill, M.F., Current practical applications of diagnostic immunohistochemistry in breast pathology (2004) American Journal of Surgical Pathology, 28 (8), pp. 1076-1091. , DOI 10.1097/01.pas.0000126780.10029.f0; 
Allinen, M., Beroukhim, R., Cai, L., Brennan, C., Lahti-Domenici, J., Huang, H., Porter, D., Polyak, K., Molecular characterization of the tumor microenvironment in breast cancer (2004) Cancer Cell, 6 (1), pp. 17-32. , DOI 10.1016/j.ccr.2004.06.010, PII S1535610804001783; 
Hu, M., Yao, J., Cai, L., Bachman, K.E., Van Den Brule, F., Velculescu, V., Polyak, K., Distinct epigenetic changes in the stromal cells of breast cancers (2005) Nature Genetics, 37 (8), pp. 899-905. , DOI 10.1038/ng1596; 
Barsky, S.H., Karlin, N.J., Myoepithelial cells: Autocrine and paracrine suppressors of breast cancer progression (2005) J Mammary Gland Biol Neoplasia, 10, pp. 249-260; 
Polyak, K., Hu, M., Do myoepithelial cells hold the key for breast tumor progression? (2005) J Mammary Gland Biol Neoplasia, 10, pp. 231-247; 
Mantyh, P.W., Clohisy, D.R., Koltzenburg, M., Hunt, S.P., Molecular mechanisms of cancer pain (2002) Nature Reviews Cancer, 2 (3), pp. 201-209; 
Ayala, G.E., Dai, H., Powell, M., Cancer-related axonogenesis and neurogenesis in prostate cancer (2008) Clin Cancer Res, 14, pp. 7593-7603; 
Wang, W., Zhao, H., Zhang, S., Al, E., Patterns of expression and function of the p75(NGFR) protein in pancreatic cancer cells and tumours (2008) Eur J Surg Oncol, , Nov 26 (in press); 
Marchesi, F., Piemonti, L., Fedele, G., The chemokine receptor CX3CR1 is involved in the neural tropism and malignant behavior of pancreatic ductal adenocarcinoma (2008) Cancer Res, 68, pp. 9060-9069; 
Zhang, S., Qi, L., Li, M., Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer (2008) J Exp Clin Cancer Res, 27, p. 62; 
Andarawewa, K.L., Motrescu, E.R., Chenard, M.-P., Gansmuller, A., Stoll, I., Tomasetto, C., Rio, M.-C., Stromelysin-3 is a potent negative regulator of adipogenesis participating to cancer cell-adipocyte interaction/crosstalk at the tumor invasive front (2005) Cancer Research, 65 (23), pp. 10862-10871. , DOI 10.1158/0008-5472.CAN-05-1231; 
Caers, J., Van Valckenborgh, E., Menu, E., Unraveling the biology of multiple myeloma disease: Cancer stem cells, acquired intracellular changes and interactions with the surrounding micro-environment (2008) Bull Cancer, 95, pp. 301-313; 
Vona-Davis, L., Rose, D.P., Adipokines as endocrine, paracrine, and autocrine factors in breast cancer risk and progression (2007) Endocrine-Related Cancer, 14 (2), pp. 189-206. , DOI 10.1677/ERC-06-0068; 
Kunduzova, O., Alet, N., Delesque-Touchard, N., Apelin/APJ signaling system: A potential link between adipose tissue and endothelial angiogenic processes (2008) FASEB J, 22, pp. 4146-4153; 
Cirillo, D., Rachiglio, A.M., La Montagna, R., Leptin signaling in breast cancer: An overview (2008) J Cell Biochem, 105, pp. 956-964; 
Jaffe, T., Schwartz, B., Leptin promotes motility and invasiveness in human colon cancer cells by activating multiple signal-transduction pathways (2008) Int J Cancer, 123, pp. 2543-2556; 
Horiguchi, A., Sumitomo, M., Asakuma, J., Asano, T., Zheng, R., Asano, T., Nanus, D.M., Hayakawa, M., Leptin Promotes Invasiveness of Murine Renal Cancer Cells Via Extracellular Signal-Regulated Kinases and Rho Dependent Pathway (2006) Journal of Urology, 176 (4), pp. 1636-1641. , DOI 10.1016/j.juro.2006.06.040, PII S0022534706014169; 
Bussard, K.M., Gay, C.V., Mastro, A.M., The bone microenvironment in metastasis; What is special about bone? (2008) Cancer Metastasis Rev, 27, pp. 41-55; 
Vakaet, L.A.M.-L., Boterberg, T., Pain control by ionizing radiation of bone metastasis (2004) International Journal of Developmental Biology, 48 (5-6), pp. 599-606. , DOI 10.1387/ijdb.041817lv, Invasion in Cancer and Embryonic Development; 
Oliveira, M.J., Van Damme, J., Lauwaet, T., De Corte, V., De Bruyne, G., Verschraegen, G., Vaneechoutte, M., Leroy, A., β-casein-derived peptides, produced by bacteria, stimulate cancer cell invasion and motility (2003) EMBO Journal, 22 (22), pp. 6161-6173. , DOI 10.1093/emboj/cdg586; 
Oliveira, M.J., Costa, A.C., Costa, A.M., Henriques, L., Suriano, G., Atherton, J.C., Machado, J.C., Figueiredo, C., Helicobacter pylori induces gastric epithelial cell invasion in a c-Met and type IV secretion system-dependent manner (2006) Journal of Biological Chemistry, 281 (46), pp. 34888-34896. , http://www.jbc.org/cgi/reprint/281/46/34888, DOI 10.1074/jbc.M607067200; 
Oliveira, M.J., Costa, A.M., Costa, A.C., Al, E., CagA associates with c-Met, E-cadherin, and p120-catenin in a multiproteic complex that suppresses Helicobacter pylori-induced cell invasive phenotype (2009) J Infect Dis, , in press; 
Miller, M.J., Wei, S.H., Parker, I., Cahalan, M.D., Two-photon imaging of lymphocyte motility and antigen response in intact lymph node (2002) Science, 296 (5574), pp. 1869-1873. , DOI 10.1126/science.1070051; 
Mullen, J.T., Wang, H., Yao, J.C., Carcinoid tumors of the duodenum (2005) Surgery, 138, pp. 971-977; 
Hoon, D.S.B., Kitago, M., Kim, J., Mori, T., Piris, A., Szyfelbein, K., Mihm Jr., M.C., Pantel, K., Molecular mechanisms of metastasis (2006) Cancer and Metastasis Reviews, 25 (2), pp. 203-220. , DOI 10.1007/s10555-006-8500-x; 
Muller, A., Homey, B., Soto, H., Ge, N., Catron, D., Buchanan, M.E., McClanahan, T., Zlotnik, A., Involvement of chemokine receptors in breast cancer metastasis (2001) Nature, 410 (6824), pp. 50-56. , DOI 10.1038/35065016; 
Hirakawa, S., Brown, L.F., Kodama, S., Paavonen, K., Alitalo, K., Detmar, M., VEGF-C-induced lymphangiogenesis in sentinel lymph nodes promotes tumor metastasis to distant sites (2007) Blood, 109 (3), pp. 1010-1017. , http://www.bloodjournal.org/cgi/reprint/109/3/1010, DOI 10.1182/blood-2006-05-021758; 
Van Den Eynden, G.G., Vandenberghe, M.K., Van Dam, P.-J.H., Colpaert, C.G., Van Dam, P., Dirix, L.Y., Vermeulen, P.B., Van Marck, E.A., Increased sentinel lymph node lymphangiogenesis is associated with nonsentinel axillary lymph node involvement in breast cancer patients with a positive sentinel node (2007) Clinical Cancer Research, 13 (18), pp. 5391-5397. , http://clincancerres.aacrjournals.org/cgi/reprint/13/18/5391, DOI 10.1158/1078-0432.CCR-07-1230; 
Dimeo, T.A., Kuperwasser, C., The evolving paradigm of tissue-specific metastasis (2006) Breast Cancer Res, 8, p. 301; 
Andre, F., Cabioglu, N., Assi, H., Sabourin, J.C., Delaloge, S., Sahin, A., Broglio, K., Cristofanilli, M., Expression of chemokine receptors predicts the site of metastatic relapse in patients with axillary node positive primary breast cancer (2006) Annals of Oncology, 17 (6), pp. 945-951. , DOI 10.1093/annonc/mdl053; 
Stessels, F., Van Den Eynden, G., Van Der Auwera, I., Salgado, R., Van Den Heuvel, E., Harris, A.L., Jackson, D.G., Vermeulen, P.B., Breast adenocarcinoma liver metastases, in contrast to colorectal cancer liver metastases, display a non-angiogenic growth pattern that preserves the stroma and lacks hypoxia (2004) British Journal of Cancer, 90 (7), pp. 1429-1436. , DOI 10.1038/sj.bjc.6601727; 
Mendes, O., Kim, H.-T., Lungu, G., Stoica, G., MMP2 role in breast cancer brain metastasis development and its regulation by TIMP2 and ERK1/2 (2007) Clinical and Experimental Metastasis, 24 (5), pp. 341-351. , DOI 10.1007/s10585-007-9071-0; 
Kaplan, R.N., Psaila, B., Lyden, D., Bone marrow cells in the {\textquoteright}pre-metastatic niche{\textquoteright}: Within bone and beyond (2006) Cancer and Metastasis Reviews, 25 (4), pp. 521-529. , DOI 10.1007/s10555-006-9036-9, Special issue on Bone Metastasis and Cancer; 
Hiratsuka, S., Watanabe, A., Aburatani, H., Maru, Y., Tumour-mediated upregulation of chemoattractants and recruitment of myeloid cells predetermines lung metastasis (2006) Nature Cell Biology, 8 (12), pp. 1369-1375. , DOI 10.1038/ncb1507, PII NCB1507; 
Kaplan, R.N., Psaila, B., Lyden, D., Niche-to-niche migration of bone-marrow-derived cells (2007) Trends in Molecular Medicine, 13 (2), pp. 72-81. , DOI 10.1016/j.molmed.2006.12.003, PII S1471491406002681; 
Pantel, K., Brakenhoff, R.H., Dissecting the metastatic cascade (2004) Nature Reviews Cancer, 4 (6), pp. 448-456; 
Vincent-Salomon, A., Bidard, F.C., Pierga, J.Y., Bone marrow micrometastasis in breast cancer: Review of detection methods, prognostic impact and biological issues (2008) Journal of Clinical Pathology, 61 (5), pp. 570-576. , DOI 10.1136/jcp.2007.046649; 
Bidard, F.C., Vincent-Salomon, A., Gomme, S., Disseminated tumor cells of breast cancer patients: A strong prognostic factor for distant and local relapse (2008) Clin Cancer Res, 14, pp. 3306-3311. , Institut Curie Breast Cancer Study Group et al; 
Ceelen, W.P., Bracke, M.E., Peritoneal minimal residual disease in colorectal cancer: Mechanisms, prevention, and treatment (2009) Lancet Oncol, 10, pp. 72-79; 
Bidard, F.C., Pierga, J.Y., Vincent-Salomon, A., A "class action" against the microenvironment: Do cancer cells cooperate in metastasis? (2008) Cancer Metastasis Rev, 27, pp. 5-10; 
Bertolini, F., Mancuso, P., Shaked, Y., Kerbel, R.S., Molecular and cellular biomarkers for angiogenesis in clinical oncology (2007) Drug Discovery Today, 12 (19-20), pp. 806-812. , DOI 10.1016/j.drudis.2007.08.011, PII S1359644607003534; 
Shields, J.D., Fleury, M.E., Yong, C., Tomei, A.A., Randolph, G.J., Swartz, M.A., Autologous Chemotaxis as a Mechanism of Tumor Cell Homing to Lymphatics via Interstitial Flow and Autocrine CCR7 Signaling (2007) Cancer Cell, 11 (6), pp. 526-538. , DOI 10.1016/j.ccr.2007.04.020, PII S1535610807001456; 
Nierodzik, M.L., Karpatkin, S., Thrombin induces tumor growth, metastasis, and angiogenesis: Evidence for a thrombin-regulated dormant tumor phenotype (2006) Cancer Cell, 10 (5), pp. 355-362. , DOI 10.1016/j.ccr.2006.10.002, PII S1535610806002868; 
Podsypanina, K., Du, Y.C., Jechlinger, M., Seeding and propagation of untransformed mouse mammary cells in the lung (2008) Science, 321, pp. 1841-1844; 
Aguirre-Ghiso, J.A., Models, mechanisms and clinical evidence for cancer dormancy (2007) Nature Reviews Cancer, 7 (11), pp. 834-846. , DOI 10.1038/nrc2256, PII NRC2256; 
Brackstone, M., Townson, J.L., Chambers, A.F., Tumour dormancy in breast cancer: An update (2007) Breast Cancer Res, 9, p. 208; 
O{\textquoteright}Reilly, M.S., Holmgren, L., Shing, Y., Chen, C., Rosenthal, R.A., Moses, M., Lane, W.S., Folkman, J., Angiostatin: A novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma (1994) Cell, 79 (2), pp. 315-328. , DOI 10.1016/0092-8674(94)90200-3; 
Norton, L., Massague, J., Is cancer a disease of self-seeding? (2006) Nature Medicine, 12 (8), pp. 875-878. , DOI 10.1038/nm0806-875, PII NM0806875; 
Bidard, F.C., (2008) Interactions en Coop{\'e}Rations Cellulaires dans le Processus M{\'e}tastatique, , MSc thesis. L{\textquoteright}Universit{\'e} Paris XI; 
Benvenuti, S., Comoglio, P.M., The MET receptor tyrosine kinase in invasion and metastasis (2007) Journal of Cellular Physiology, 213 (2), pp. 316-325. , DOI 10.1002/jcp.21183; 
Mareel, M., Leroy, A., Clinical, cellular, and molecular aspects of cancer invasion (2003) Physiological Reviews, 83 (2), pp. 337-376; 
Christofori, G., New signals from the invasive front (2006) Nature, 441 (7092), pp. 444-450. , DOI 10.1038/nature04872, PII NATURE04872; 
Sabbah, M., Emami, S., Redeuilh, G., Molecular signature and therapeutic perspective of the epithelial-to-mesenchymal transitions in epithelial cancers (2008) Drug Resist Updat, 11, pp. 123-151; 
Thiery, J.P., Sleeman, J.P., Complex networks orchestrate epithelial-mesenchymal transitions (2006) Nature Reviews Molecular Cell Biology, 7 (2), pp. 131-142. , DOI 10.1038/nrm1835, PII NRM1835; 
Yang, J., Weinberg, R.A., Epithelial-Mesenchymal Transition: At the Crossroads of Development and Tumor Metastasis (2008) Developmental Cell, 14 (6), pp. 818-829. , DOI 10.1016/j.devcel.2008.05.009, PII S1534580708002098; 
Christiansen, J.J., Rajasekaran, A.K., Reassessing epithelial to mesenchymal transition as a prerequisite for carcinoma invasion and metastasis (2006) Cancer Research, 66 (17), pp. 8319-8326. , DOI 10.1158/0008-5472.CAN-06-0410; 
Brabletz, T., Jung, A., Spaderna, S., Hlubek, F., Kirchner, T., Migrating cancer stem cells - An integrated concept of malignant tumour progression (2005) Nature Reviews Cancer, 5 (9), pp. 744-749. , DOI 10.1038/nrc1694, PII N1694; 
Tarin, D., The fallacy of epithelial mesenchymal transition in neoplasia (2005) Cancer Research, 65 (14), pp. 5996-6000. , DOI 10.1158/0008-5472.CAN-05-0699; 
Garber, K., Epithelial-to-mesenchymal transition is important to metastasis, but questions remain (2008) J Natl Cancer Inst, 100, pp. 232-239; 
De Wever, O., Derycke, L., Hendrix, A., De Meerleer, G., Godeau, F., Depypere, H., Bracke, M., Soluble cadherins as cancer biomarkers (2007) Clinical and Experimental Metastasis, 24 (8), pp. 685-697. , DOI 10.1007/s10585-007-9104-8; 
Bellahcene, A., Castronovo, V., Ogbureke, K.U.E., Fisher, L.W., Fedarko, N.S., Small integrin-binding ligand N-linked glycoproteins (SIBLINGs): Multifunctional proteins in cancer (2008) Nature Reviews Cancer, 8 (3), pp. 212-226. , DOI 10.1038/nrc2345, PII NRC2345; 
Gimona, M., Buccione, R., Adhesions that mediate invasion (2006) International Journal of Biochemistry and Cell Biology, 38 (11), pp. 1875-1892. , DOI 10.1016/j.biocel.2006.05.003, PII S1357272506001580; 
Gimona, M., Buccione, R., Courtneidge, S.A., Assembly and biological role of podosomes and invadopodia (2008) Curr Opin Cell Biol, 20, pp. 235-241; 
Lopez-Otin, C., Matrisian, L.M., Emerging roles of proteases in tumour suppression (2007) Nature Reviews Cancer, 7 (10), pp. 800-808. , DOI 10.1038/nrc2228, PII NRC2228; 
Martin, M.D., Matrisian, L.M., The other side of MMPs: Protective roles in tumor progression (2007) Cancer and Metastasis Reviews, 26 (3-4), pp. 717-724. , DOI 10.1007/s10555-007-9089-4, Forty Years of Metastasis Research: A Tribute to Dr. Isaiah J. Fidler; 
Sahai, E., Mechanisms of cancer cell invasion (2005) Current Opinion in Genetics and Development, 15 (1), pp. 87-96. , DOI 10.1016/j.gde.2004.12.002, PII S0959437X04001881, Oncogenes and Cell Proliferation; 
Wolf, K., Mazo, I., Leung, H., Engelke, K., Von Andrian, U.H., Deryugina, E.I., Strongin, A.Y., Friedl, P., Compensation mechanism in tumor cell migration: Mesenchymal-amoeboid transition after blocking of pericellular proteolysis (2003) Journal of Cell Biology, 160 (2), pp. 267-277. , DOI 10.1083/jcb.200209006; 
Wolf, K., Friedl, P., Molecular mechanisms of cancer cell invasion and plasticity (2006) Br J Dermatol, 154 (SUPPL. 1), pp. 11-15; 
Yilmaz, M., Christofori, G., Lehembre, F., Distinct mechanisms of tumor invasion and metastasis (2007) Trends in Molecular Medicine, 13 (12), pp. 535-541. , DOI 10.1016/j.molmed.2007.10.004, PII S1471491407001888; 
Wicki, A., Christofori, G., The potential role of podoplanin in tumour invasion (2007) British Journal of Cancer, 96 (1), pp. 1-5. , DOI 10.1038/sj.bjc.6603518, PII 6603518; 
Yamaguchi, H., Condeelis, J., Regulation of the actin cytoskeleton in cancer cell migration and invasion (2007) Biochimica et Biophysica Acta - Molecular Cell Research, 1773 (5), pp. 642-652. , DOI 10.1016/j.bbamcr.2006.07.001, PII S0167488906001558; 
Sahai, E., Garcia-Medina, R., Pouyssegur, J., Vial, E., Smurf1 regulates tumor cell plasticity and motility through degradation of RhoA leading to localized inhibition of contractility (2007) Journal of Cell Biology, 176 (1), pp. 35-42. , http://www.jcb.org/cgi/reprint/176/1/35, DOI 10.1083/jcb.200605135; 
Gentile, A., Trusolino, L., Comoglio, P.M., The Met tyrosine kinase receptor in development and cancer (2008) Cancer Metastasis Rev, 27, pp. 85-94; 
Lengyel, E., Sawada, K., Salgia, R., Tyrosine kinase mutations in human cancer (2007) Current Molecular Medicine, 7 (1), pp. 77-84. , http://www.ingentaconnect.com/content/ben/cmm/2007/00000007/00000001/ art00007, DOI 10.2174/156652407779940486; 
Funasaka, T., Raz, A., The role of autocrine motility factor in tumor and tumor microenvironment (2007) Cancer and Metastasis Reviews, 26 (3-4), pp. 725-735. , DOI 10.1007/s10555-007-9086-7, Forty Years of Metastasis Research: A Tribute to Dr. Isaiah J. Fidler; 
Hehlgans, S., Haase, M., Cordes, N., Signalling via integrins: Implications for cell survival and anticancer strategies (2007) Biochimica et Biophysica Acta - Reviews on Cancer, 1775 (1), pp. 163-180. , DOI 10.1016/j.bbcan.2006.09.001, PII S0304419X06000588; 
Douma, S., Van Laar, T., Zevenhoven, J., Suppression of anoikis and induction of metastasis by the neurotrophic receptor TrkB (2004) Nature, 430, pp. 1034-1039; 
Geiger, T.R., Peeper, D.S., The neurotrophic receptor TrkB in anoikis resistance and metastasis: A perspective (2005) Cancer Research, 65 (16), pp. 7033-7036. , DOI 10.1158/0008-5472.CAN-05-0709; 
Sandri, M.T., Circulating tumor cells (2007) IEO-Scientific Report 2007-ongoing Research 2008, pp. 192-194; 
Gnerlich, J., Jeffe, D.B., Deshpande, A.D., Beers, C., Zander, C., Margenthaler, J.A., Surgical removal of the primary tumor increases overall survival in patients with metastatic breast cancer: Analysis of the 1988-2003 SEER data (2007) Annals of Surgical Oncology, 14 (8), pp. 2187-2194. , DOI 10.1245/s10434-007-9438-0; 
Salama, J.K., Chmura, S.J., Mehta, N., An initial report of a radiation dose-escalation trial in patients with one to five sites of metastatic disease (2008) Clin Cancer Res, 14, pp. 5255-5259; 
Dolle, L., Depypere, H.T., Bracke, M.E., Anti-invasive and anti-metastasis strategies: New roads, new tools and new hopes (2006) Current Cancer Drug Targets, 6 (8), pp. 729-751. , http://docstore.ingenta.com/cgi-bin/ds_deliver/1/u/d/ISIS/33724480.1/ben/ ccdt/2006/00000006/00000008/art00007/9C146EDF9740EFD9116485319359E89FF5E8D1E22C. pdf?link=http://www.ingentaconnect.com/error/delivery\&format=pdf, DOI 10.2174/156800906779010263; 
Fox, S.B., Generali, D.G., Harris, A.L., Breast tumour angiogenesis (2007) Breast Cancer Res, 9, p. 216; 
Shaked, Y., Kerbel, R.S., Antiangiogenic strategies on defense: On the possibility of blocking rebounds by the tumor vasculature after chemotherapy (2007) Cancer Research, 67 (15), pp. 7055-7058. , http://cancerres.aacrjournals.org/cgi/reprint/67/15/7055, DOI 10.1158/0008-5472.CAN-07-0905; 
Formenti, S.C., Demaria, S., Local control by radiotherapy: Is that all there is? (2008) Breast Cancer Res, 10, p. 215; 
Husemann, Y., Geigl, J.B., Schubert, F., Musiani, P., Meyer, M., Burghart, E., Forni, G., Klein, C.A., Systemic Spread Is an Early Step in Breast Cancer (2008) Cancer Cell, 13 (1), pp. 58-68. , DOI 10.1016/j.ccr.2007.12.003, PII S1535610807003728; 
Denys, H., Derycke, L., Hendrix, A., Differential impact of TGF-beta and EGF on fibroblast differentiation and invasion reciprocally promotes colon cancer cell invasion (2008) Cancer Lett, 266, pp. 263-274; 
Milas, L., Mason, K., Liao, Z., Nieder, C., Milas, L., Ang, K.K., Role of cyclooxygenase-2 (COX-2) and its inhibition in tumor biology and radiotherapy (2003) Modification of Radiation Response, pp. 241-258. , Springer Berlin; 
Opdenakker, G., Van Damme, J., The countercurrent principle in invasion and metastasis of cancer cells. Recent insights on the roles of chemokines (2004) International Journal of Developmental Biology, 48 (5-6), pp. 519-527. , DOI 10.1387/ijdb.041796go, Invasion in Cancer and Embryonic Development; 
Nugent, J.L., Bunn Jr., P.A., Matthews, M.J., CNS metastases in small cell bronchogenic carcinoma. Increasing frequency and changing pattern with lengthening survival (1979) Cancer, 44 (5), pp. 1885-1893; 
Camphausen, K., Moses, M.A., Beecken, W.-D., Khan, M.K., Folkman, J., O{\textquoteright}Reilly, M.S., Radiation therapy to a primary tumor accelerates metastatic growth in mice (2001) Cancer Research, 61 (5), pp. 2207-2211; 
Ramaswamy, S., Ross, K.N., Lander, E.S., Golub, T.R., A molecular signature of metastasis in primary solid tumors (2003) Nature Genetics, 33 (1), pp. 49-54. , DOI 10.1038/ng1060; 
Chen, Y.-J., Lin, S.-C., Kao, T., Chang, C.-S., Hong, P.-S., Shieh, T.-M., Chang, K.-W., Genome-wide profiling of oral squamous cell carcinoma (2004) Journal of Pathology, 204 (3), pp. 326-332. , DOI 10.1002/path.1640; 
Perou, C.M., Sorile, T., Eisen, M.B., Van De Rijn, M., Jeffrey, S.S., Ress, C.A., Pollack, J.R., Botstein, D., Molecular portraits of human breast tumours (2000) Nature, 406 (6797), pp. 747-752. , DOI 10.1038/35021093; 
LaTulippe, E., Satagopan, J., Smith, A., Scher, H., Scardino, P., Reuter, V., Gerald, W.L., Comprehensive gene expression analysis of prostate cancer reveals distinct transcriptional programs associated with metastatic disease (2002) Cancer Research, 62 (15), pp. 4499-4506; 
Lapointe, J., Li, C., Giacomini, C.P., Salari, K., Huang, S., Wang, P., Ferrari, M., Pollack, J.R., Genomic profiling reveals alternative genetic pathways of prostate tumorigenesis (2007) Cancer Research, 67 (18), pp. 8504-8510. , http://cancerres.aacrjournals.org/cgi/reprint/67/18/8504, DOI 10.1158/0008-5472.CAN-07-0673; 
Lips, E.H., Van Eijk, R., De Graaf, E.J., Integrating chromosomal aberrations and gene expression profiles to dissect rectal tumorigenesis (2008) BMC Cancer, 8, p. 314; 
Cromer, A., Carles, A., Millon, R., Ganguli, G., Chalmel, F., Lemaire, F., Young, J., Wasylyk, B., Identification of genes associated with tumorigenesis and metastatic potential of hypopharyngeal cancer by microarray analysis (2004) Oncogene, 23 (14), pp. 2484-2498. , DOI 10.1038/sj.onc.1207345; 
Couvelard, A., Hu, J., Steers, G., O{\textquoteright}Toole, D., Sauvanet, A., Belghiti, J., Bedossa, P., Pezzella, F., Identification of Potential Therapeutic Targets by Gene-Expression Profiling in Pancreatic Endocrine Tumors (2006) Gastroenterology, 131 (5), pp. 1597-1610. , DOI 10.1053/j.gastro.2006.09.007, PII S0016508506020014; 
Bertucci, F., Finetti, P., Rougemont, J., Charafe-Jauffret, E., Nasser, V., Loriod, B., Camerlo, J., Viens, P., Gene expression profiling for molecular characterization of inflammatory breast cancer and prediction of response to chemotherapy (2004) Cancer Research, 64 (23), pp. 8558-8565. , DOI 10.1158/0008-5472.CAN-04-2696
},
	month = {2009///},
	pages = {599 - 622},
	abstract = {Malignant tumors invade and metastasize. They consist of cancer cells, evolving through genetic and epigenetic modulation, mixed with tumor-associated host cells, emerging from resident or bone marrow-derived precursors. These cells establish ecosystems to activate cellular programs for local invasion and distant metastasis. Characteristic of such malignancy-related activities is communication inside ecosystems between cells, ligands, receptor protein complexes, and signaling pathways as well as between ecosystems comprising the primary tumor, lymph node and distant metastasis, bone marrow and blood and lymph circulation. Complexity is another characteristic, resulting from: heterogeneity of the cell populations; the numbers of promoter and suppressor genes, their levels of regulation, and the pleiotropic activities of their products; biological redundancy of the molecular mechanisms underpinning invasion-related activities. Clinical attention is paid to putative new targets, namely host cells, individual molecules and their signaling pathways, as well as the effects of current treatment on invasion and metastasis. {\textcopyright} 2009 Springer-Verlag.},
	keywords = {bone marrow, cancer cell, cancer invasion, carcinogenesis, cell activity, Circulation, ecosystem, epigenetics, extracellular matrix, gene control, host cell, human, Humans, lymph node metastasis, metastasis, Metastatic site, microarray analysis, Molecular biology, molecular imaging, Neoplasm Invasiveness, Neoplasm Metastasis, Neoplasms, nonhuman, pleiotropy, primary tumor, priority journal, Promoter and suppressor genes, promoter region, review, signal transduction, suppressor gene, Therapy guidance, Tumor-associated host cells},
	isbn = {09456317 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-67649116432\&partnerID=40\&md5=4c02a29f53858424b4c3191dcd86256c},
	author = {Mareel, M. and Oliveira, M.J. and Madani, I.}
}
@article {2306,
	title = {Cationic liposome-DNA complexes as gene delivery vectors: Development and behaviour towards bone-like cells},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {5},
	year = {2009},
	note = {Cited By (since 1996): 17Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2009.02.019PubMed ID: 19332382Language of Original Document: EnglishCorrespondence Address: Ferraz, M.P.; CEBIMED, Faculdade de Ci{\^e}ncias da Sa{\'u}, Universidade Fernando Pessoa, Rua Carlos da Maia, 296, 4200-150 Porto, Portugal; email: mpferraz@ufp.ptChemicals/CAS: 1,2 dioleoyl 3 trimethylammoniopropane, 144189-73-1; DNA, 9007-49-2; transferrin, 82030-93-1; DNA, 9007-49-2; LiposomesReferences: Luo, J., Sun, M.H., Kang, Q., Peng, Y., Jiang, W., Luu, H.H., Gene therapy for bone regeneration (2005) Curr Gene Ther, 5 (2), pp. 167-179;Oreffo, R.O.C., Triffitt, J.T., Future potentials for using osteogenic stem cells and biomaterials in orthopedics (1999) Bone, 25 (2 SUPPL. 1), pp. 5S-9S; 
Laurencin, C.T., Attawia, M.A., Lu, L.Q., Borden, M.D., Lu, H.H., Gorum, W.J., Poly(lactide-co-glycolide)/hydroxyapatite delivery of BMP-2-producing cells: a regional gene therapy approach to bone regeneration (2001) Biomaterials, 22 (11), pp. 1271-1277; 
Katz, J.L., (1996) Applications of materials in medicine and dentistry: orthopedic applications, , Academic Press, San Diego, CA p. 335-46; 
Bucholz, R., Nonallograft osteoconductive bone grafts substitutes (2002) Clin Orthop, 395, pp. 44-52; 
Kirker-Head, C.A., Potential applications and delivery strategies of bone morphogenetic proteins (2000) Adv Drug Deliv Rev, 43, pp. 65-92; 
Chen, Y., Luk, K.D.K., Cheung, K.M.C., Xu, R., Lin, M.C., Lu, W.W., Gene therapy for new bone formation using adeno-associated viral bone morphogenetic protein-2 vectors (2003) Gene Ther, 10 (16), pp. 1345-1353; 
Tseng, W.-C., Huang, L., Liposome-based gene therapy (1998) Res Focus, 1 (5); 
Leach, J.K., Mooney, D., Bone engineering by controlled delivery of osteoinductive molecules and cells (2004) Expert Opin, 4 (7); 
Dragoo, J.L., Choi, J.Y., Lieberman, J.R., Huang, J., Zuk, P.A., Zhang, J., Bone induction by BMP-2 transduced stem cells derived from human fat (2003) J Orthop Res, 21 (4), pp. 622-629; 
Eliyahu, H., Barenholz, Y., Domb, A.J., Polymers for DNA delivery (2005) Molecules, 10, pp. 34-64; 
Penque, D., Terapia g{\'e}nica: um objectivo ou uma realidade (2000) Boletim Biotecnol, 66, pp. 16-19; 
Winn, S.R., Hu, Y., Sfeir, C., Hollinger, J.O., Gene therapy approaches for modulating bone regeneration (2000) Adv Drug Deliv Rev, 42 (1-2), pp. 121-138; 
Lima, N., Mota, M., (2003) Biotecnologia, fundamentos e aplica{\c c}{\~o}es, , Lidel, Lisboa p. 368-73; 
Luo, D., Saltzman, W.M., Synthetic DNA delivery systems (2000) Nat Biotechnol, 18, pp. 33-37; 
Kikuchi, H., Suzuki, N., Ebihara, K., Morita, H., Ishii, Y., Kikuchi, A., Gene delivery using liposome technology (1999) J Control Release, 62 (1-2), pp. 269-277; 
Liu, F., Huang, L., Development of non-viral vectors for systemic gene delivery (2002) J Control Release, 78 (1-3), pp. 259-266; 
Girao da Cruz, M.T., Simoes, S., Pires, P.P.C., Nir, S., Pedroso de Lima, M.C., Kinetic analysis of the initial steps involved in lipoplex-cell interactions: effect of various factors that influence transfection activity (2001) Biochim Biophys Acta (BBA) - Biomembr, 1510 (1-2), pp. 136-151; 
Zuidam, N.J., Barenholz, Y., Electrostatic and structural properties of complexes involving plasmid DNA and cationic lipids commonly used for gene delivery (1998) Biochim Biophys Acta (BBA) - Biomembr, 1368 (1), pp. 115-128; 
Tros de Ilarduya, C., Arangoa, M.A., Moreno-Aliaga, M.J., Duzgunes, N., Enhanced gene delivery in vitro and in vivo by improved transferrin-lipoplexes (2002) Biochim Biophys Acta (BBA) - Biomembr, 1561 (2), pp. 209-221; 
Simoes, S., Pires, P., Cruz, M.T.G.d., Duzgunes, N., Pedroso de Lima, M.C., Gene delivery by cationic liposome-DNA complexes containing transferrin or serum albumin (2003) Methods Enzymol, 373, pp. 369-383; 
Woo-Jeong, C., Jin-Ki, K., Sung-Hee, C., Jeong-Sook, P., Woong Shick, A., Kima, C.-K., Low toxicity of cationic lipid-based emulsion for gene transfer (2004) Biomaterials, 25 (27), pp. 5893-5903; 
Koe, G.S., Way, H.L., Quentingco, G.M., Smith, J.G., Wedeking, T., Niven, R.W., The effect of mixing on the formation of DNA/liposome complexes (1997) Pharmacy, p. 14; 
Lasic, D.D., Templeton, N.S., Liposomes in gene therapy (1996) Adv Drug Deliv Rev, 20 (2-3), pp. 221-266; 
Pedroso de Lima, M.C., Simoes, S., Pires, P., Faneca, H., Duzgunes, N., Cationic lipid-DNA complexes in gene delivery: from biophysics to biological applications (2001) Adv Drug Deliv Rev, 47 (2-3), pp. 277-294; 
Hirsch-Lerner, D., Zhang, M., Eliyahu, H., Ferrari, M.E., Wheeler, C.J., Barenholz, Y., Effect of "helper lipid" on lipoplex electrostatics (2005) Biochim Biophys Acta (BBA) - Biomembr, 1714 (2), pp. 71-84; 
Torricelli, P., Fini, M., Giavaresi, G., Borsari, V., Carpi, A., Nicolini, A., Comparative interspecies investigation on osteoblast cultures: data on cell viability and synthetic activity (2003) Biomed Pharmacother, 57 (1), pp. 57-62; 
Corsi, K., Chellat, F., Yahia, L., Fernandes, J.C., Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles (2003) Biomaterials, 24 (7), pp. 1255-1264; 
Mislick, K.A., Baldeschwieler, J.D., Evidence for role of proteoglycans in cation-mediated gene transfer (1996) Proc Natl Acad Sci USA, 93 (22), pp. 12349-12354; 
Duzgunes, N., Nir, S., Mechanisms and kinetics of liposome-cell interactions (1999) Adv Drug Deliv Rev, 40 (1-2), pp. 3-18; 
Radwan Almofti, M., Harashima, H., Shinohara, Y., Almofti, A., Baba, Y., Kiwada, H., Cationic liposome-mediated gene delivery: biophysical study and mechanism of internalization (2003) Arch Biochem Biophys, 410 (2), pp. 246-253; 
Sim{\~o}es, S., Filipe, A., Faneca, H., Mano, M., Penacho, N., Duzgunes, N., Cationic liposomes for gene delivery (2005) Expert Opin, 2 (2); 
Rejman, J., Bragonzi, A., Conese, M., Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes (2005) Mol Ther, 12 (3), pp. 468-474
},
	month = {2009///},
	pages = {2142 - 2151},
	abstract = {Modulation of the biological pathways responsible for fracture repair and osteogenisis may accelerate regeneration. Gene therapy is an alternative method for the release of osteogenisis-stimulating proteins into tissues. The development of vectors for gene release is still a problem in terms of ethics and techniques. In this work we evaluated whether cationic liposomes constitute a valuable strategy for the release of genetic material into bone tissue cells as non-viral vectors. Liposomes were prepared with 1,2-dioleoyl-3-trimethylammonium propane (DOTAP)-2-dioleoyl-sn-glycero-3-phosphatidylethanolamine and DOTAP-cholesterol, and characterized according to their size, zeta potential, DNA protection capacity and cytotoxicity. Transfection studies were also carried out using pCMVβ-gal plasmid in two osteoblastic cell lines (MG63 and MC3T3-E1) and in the 294T line, varying the charge ratio and the applied DNA dose. Inclusion of transferrin to increase the expression was also tested. The results suggest that there is great dependency between the transfection activity and the lipid formulation, the charge ratios of the complexes, the applied DNA dose and the cell type. There were even some differences concerning both osteoblastic lines under study. The cells of the MC3T3-E1 line present greater expression levels than the cells of the MG-63 line. The conjugation of the transferrin with the complexes contributes to the increase in transfection levels, possibly due to an increase in internalization of complexes. It is thus a good strategy for inducing the expression of specific genes in osteoblast-like cells. {\textcopyright} 2009 Acta Materialia Inc.},
	keywords = {1,2 dioleoyl 3 trimethylammoniopropane, animal cell, Animals, article, bone cell, bone development, bone tissue, Cationic liposomes, Cell Line, cell type, complex formation, controlled study, Cytotoxicity, DNA, drug mechanism, gene expression regulation, Gene therapy, Gene transfer, genetic transfection, Genetic Vectors, human, human cell, internalization, lipoplex, liposome, Liposomes, materials testing, Mice, nonhuman, nonviral gene delivery system, osteoblast, Osteoblasts, Osteogenesis, Particle Size, plasmid, priority journal, Transfection, transferrin, Zeta potential},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-67349188484\&partnerID=40\&md5=b675ab7e6e97523c5d8923fa6e7f0d8b},
	author = {Oliveira, A.C. and Ferraz, M.P. and Monteiro, F.J. and Sim{\~o}es, S.}
}
@book {2302,
	title = {Cell division detection on the arabidopsis thaliana root},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {5524 LNCS},
	year = {2009},
	note = {Conference code: 77027Cited By (since 1996): 5Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-02172-5_23Language of Original Document: EnglishCorrespondence Address: Marcuzzo, M.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Sinal e Imagem, Campus FEUPReferences: Campilho, A., Garcia, B., Toorn, H., Wijk, H., Campilho, A., Scheres, B., Time-lapse analysis of stem-cell divisions in the arabidopsis thaliana root meristem (2006) The Plant Journal, 48, pp. 619-627;Iwamoto, A., Satoh, D., Furutani, M., Maruyama, S., Ohba, H., Sugiyama, M., Insight into the basis of root growth in arabidopsis thaliana provided by a simple mathematical model (2006) J. Plant Res, 119, pp. 85-93; 
Roberts, T., Mckenna, S., Wuyts, N., Valentine, T., Bengough, A.: Performance of low-level motion estimation methods for confocal microscopy of plant cells in vivo. In: Motion 2007, p. 13 (2007)Beemster, G., Baskin, T., Analysis of cell division and elongation underlying the developmental acceleration of root growth in arabidopsis thaliana (1998) Plant Physiology, 116, pp. 1515-1526; 
Chen, X., Zhou, X., Wong, S.T.C., Automated segmentation, classification, and tracking of cancer cell nuclei in time-lapse microscopy (2006) IEEE Trans. on Biomedical Engineering, 53 (4), pp. 762-766; 
Mao, K.Z., Zhao, P., Tan, P., Supervised learning-based cell image segmentation for p53 immunohistochemistry (2006) IEEE Trans. on Biomedical Engineering, 53 (6), pp. 1153-1163; 
Yang, X., Li, H., Zhou, X., Nuclei segmentation using marker-controlled watershed, tracking using mean-shift, and kalman filter in time-lapse microscopy (2006) IEEE Trans. on Circuits and Systems I-Regul. Papers, 53 (11), pp. 2405-2414; 
Harder, N., M-Bermudez, F., Godinez, W., Ellenberg, J., Eils, R., Rohr, K., Automated analysis of mitotic cell nuclei in 3d fluorescence microscopy image sequences (2008) Workshop on Bio-Image Informatics: Biological Imaging, Computer Vision and Data Mining; 
Kobatake, H., Hashimoto, S., Convergence index filter for vector fields (1999) IEEE Trans. on Image Processing, 8 (8); 
Wei, J., Hagihara, Y., Kobatake, H., Detection of rounded opacities on chest radiographs using convergence index filter (1999) Proceedings of the Int. Conference on Image Analysis and Processing, pp. 757-761; 
Pereira, C.S., Fernandes, H., Mendon{\c c}a, A.M., Campilho, A.C.: Detection of lung nodule candidates in chest radiographs. In: Mart{\'\i}, J., Bened{\'\i}, J.M., Mendon{\c c}a, A.M., Serrat, J. (eds.) IbPRIA 2007. LNCS, 4478, pp. 170-177. Springer, Heidelberg (2007)Marcuzzo, M., Quelhas, P., Campilho, A., Mendon{\c c}a, A.M., Campilho, A., A hybrid approach for cell image segmentation (2008) LNCS, 5112, pp. 739-749. , Campilho, A, Kamel, M.S, eds, ICIAR 2008, Springer, Heidelberg
Sponsors: International Association for Pattern Recognition (IAPR); Fundacao para a Ciencia e a Tecnologia (FCT)},
	month = {2009///},
	pages = {168 - 175},
	address = {Povoa de Varzim},
	abstract = {The study of individual plant cells and their growth structure is an important focus of research in plant genetics. To obtain development information at cellular level, researchers need to perform in vivo imaging of the specimen under study, through time-lapse confocal microscopy. Within this research field it is important to understand mechanisms like cell division and elongation of developing cells. We describe a tool to automatically search for cell division in the Arabidopsis thaliana using information of nuclei shape. The nuclei detection is based on a convergence index filter. Cell division detection is performed by an automatic classifier, trained through cross-validation. The results are further improved by a stability criterion based on the Mahalanobis distance of the shape of the nuclei through time. With this approach, we can achieve a correct detection rate of 94.7\%. {\textcopyright} 2009 Springer Berlin Heidelberg.},
	keywords = {A-stability, Arabidopsis thaliana, Automatic classifiers, Bioinformatics, Cell divisions, Cell membranes, cell proliferation, Cellular levels, confocal microscopy, Cross validation, Detection rates, Growth structures, Image analysis, In-plants, In-Vivo imaging, Mahalanobis distances, Pattern recognition, Plant cells, research, Research fields, Stability criteria},
	isbn = {03029743 (ISSN); 3642021719 (ISBN); 9783642021718 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-68749115481\&partnerID=40\&md5=2c491950d3b00a9dd873aeaefdf5edcb},
	author = {Marcuzzo, M. and Guichard, T. and Quelhas, P. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@book {2319,
	title = {Cellular alignment induction during early in vitro culture stages using micropatterned glass coatings produced by sol-gel process},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {396-398},
	year = {2009},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: KEMAEdoi: 10.4028/0-87849-353-0.303Language of Original Document: EnglishCorrespondence Address: Pelaez-Vargas, A.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do PortoPortugal; email: alejopv@ineb.up.ptReferences: Warashina, H., Sakano, S., Kitamura, S., Yamauchi, K.I., (2003) Biomaterials, 24, pp. 3655-3661;Scarano, A., Piattelli, M., Caputi, S., Favero, G.A., Piattelli, A., (2004) J Periodontol, 75, pp. 292-296; 
Manicone, P.F., Rossi Iommetti, P., Raffaelli, L., (2007) J Dent, 35, pp. 819-826; 
Covacci, V., Bruzzese, N., Maccauro, G., Andreassi, C., (1999) Biomaterials, 20, pp. 371-376; 
Silva, V.V., Lameiras, F.S., Lobato, Z.I., (2002) J Biomed Mater Res, 63, pp. 583-590; 
Oliva, J., Oliva, X., Oliva, J., (2007) Int J Oral Maxillofac Implants, 22, pp. 430-435; 
Klinge, B., Hultin, M., Berglundh, T., (2005) Dent Clin North Am, 49, pp. 661-676; 
Wennerberg, A., Albrektsson, T., Andersson, B., Krol, J.J., (1995) Clin Oral Impl Res, 6, pp. 24-30; 
Brunette, D.M., Kenner, G.S., Gould, T.R., (1983) J Dent Res, 62, pp. 1045-1048; 
Pelaez, A., Pareja, A., Garcia, C., Marquez, M., (2005) Key Eng Mater, 284, pp. 593-596; 
Curtis, A., Wilkinson, C., (1997) Biomaterials, 18, pp. 1573-1583; 
Ito, Y., (1999) Biomaterials, 20, pp. 2333-2342; 
Tan, J., Saltzman, W.M., (2004) Biomaterials, 25, pp. 3593-3601; 
Gallego, D., Ferrell, N., Sun, Y., Hansford, D.J., (2008) Mater Sci Eng C, 28, pp. 353-358; 
Xia, Y., Whitesides, G.M., (1998) Angew. Chem. Int. Ed, 37, pp. 550-575
},
	month = {2009///},
	pages = {303 - 306},
	abstract = {Cell behaviour such as adhesion, morphology, proliferation and functional activity are highly influenced by surface properties including hydrophobicity, roughness, texture and morphology. These surface properties may be controlled using a mixture of additive coating techniques to produce glass coatings by sol-gel process and soft lithography on dental ceramics. The purpose of this work was to compare cell adhesion and early orientation of Human Bone Marrow (HBM) cells cultured on micro-patterned (micro-PGC) and on flat glass coatings (FGC) produced by sol-gel processing. Spin coating was used to apply SiO                        2 flat coatings on glass substrates as model surfaces. Photolithography was applied to produce master patterns with microscale dimensions. A moulding technique was used to print micropatterned SiO 
                        2 glass coatings produced by a sol-gel process. The coatings were then sintered, sterilized and cultured with HBM cells derived from primary cultures, using a standardized protocol, for 1 and 7 days. Cell morphology and orientation were observed using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Flat and MPGC with line shaped features were produced. Cells presented a typical osteoblastic morphology on flat surfaces while slimmer, preferentially oriented and more elongated morphologies could be seen on line micro-patterned surfaces. HBM cells cultured on flat glass coatings showed increased tendency to spread and to assume more randomized proliferation when compared to the cells on the micro-patterned glass coatings. Micro-patterned glass coatings showed higher orientation control and smaller delay in the rate of proliferation, in early stages of in vitro culture as compared to flat coatings. These preliminary studies revealed that Micro-PGC induce significant morphological changes and controlled orientation of HBM cells during early stages of cell proliferation.
},
	keywords = {Adhesion, cell adhesion, Cell behaviours, Cell culture, Cell morphologies, cell proliferation, Cells, Coatings, Colloids, Confocal laser scanning microscopies, confocal microscopy, Contact guidance, Controlled orientations, cytology, Dental ceramics, Dental Materials, Flat glass, Flat surfaces, Functional activities, Gelation, Gels, glass, Glass ceramics, Glass coatings, Glass substrates, In-vitro, Micro-patterned, Micro-patterned surfaces, Micro-scale, Micromachining, Micropatterning, Model surfaces, Morphological changes, morphology, Nanostructured materials, Orientation controls, Primary cultures, Scanning, Scanning electron microscopy, Scanning electrons, SEM, Silicon compounds, Soft lithographies, Sol-gel, Sol-gel process, Sol-gel processing, Sol-gels, Sols, Surface Properties, Thermal spraying, Trace analysis},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-58849141765\&partnerID=40\&md5=7a4c975e741442308a8db69108047673},
	author = {Pelaez-Vargas, A. and Ferrell, N. and Fernandes, M.H. and Hansford, D.J. and Monteiro, F.J.}
}
@book {2301,
	title = {Chromatographic pattern recognition using optimized one-class classifiers},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {5524 LNCS},
	year = {2009},
	note = {Conference code: 77027Export Date: 21 November 2012Source: Scopusdoi: 10.1007/978-3-642-02172-5_58Language of Original Document: EnglishCorrespondence Address: Sousa, A. V.; Instituto de Engenharia Biom{\'e}dica, Rua Roberto Frias, Porto 4200-465, Portugal; email: ats@isep.ipp.ptReferences: Skurichina, M., Raudys, S., Duin, R.P.W., K-Nearest Neighbors Directed Noise Injection in Multilayer Perceptron Training (2000) IEEE Transactions on Neural Networks, 11;Chawla, N.V., Japkowicz, N., Kolcz, A., Editorial: Special Issue: Learning from Imbal-anced Data Sets (2004) Sigkdd Explorations, 6, pp. 1-6; 
Tax, D.M.J., (2001) One-class Classification; Concept-learning in the Absence of Counterexamples, , Ph.D. thesis: Delft University of Technology; 
Durand, G., Seta, N., Protein Glycosylation and Diseases: Blood and Urinary Oligosac-charides as Markers for Diagnosis and Therapeutic Monitoring (2000) Clin. Chem, 46, pp. 795-805; 
Sewell, A.C., Urinary Oligosaccharides. Techniques in Diagnostic Human (1991) Biochemical Genetics, pp. 219-231; 
Pekalska, E., Duin, R.P.W., Dissimilarity Representations Allow for Building Good Classifiers (2002) Pattern Recogn. Lett, 23, pp. 943-956; 
Sousa, A.V., Mendon{\c c}a, A.M., Campilho, A., Dissimilarity-based Classification of Chromatographic Profiles (2008) Pattern Analysis and Applications, 11 (3-4), pp. 409-423; 
Sousa, A.V., Mendon{\c c}a, A.M., Campilho, A., Minimizing the Imbalance Problem in Chromatographic Profile Classification with One-class Classifiers (2008) LNCS, 5112, pp. 413-422. , Campilho, A, Kamel, M.S, eds, ICIAR 2008, Springer, Heidelberg; 
Sousa, A.V., Mendon{\c c}a, A.M., Campilho, A., Chromatographic Pattern Classification (2008) IEEE Trans. on Biomedical Engineering, 55 (6), pp. 1687-1696; 
Visa, S., Ralescu, A., Learning Imbalanced and Overlapping Classes Using Fuzzy Sets (2003) Workshop on Learning from Imbalanced Datasets II, ICML, , Washington DC
Sponsors: International Association for Pattern Recognition (IAPR); Fundacao para a Ciencia e a Tecnologia (FCT)},
	month = {2009///},
	pages = {449 - 456},
	address = {Povoa de Varzim},
	abstract = {This paper presents a method for automating the selection of the rejection rate of one-class classifiers aiming at optimizing the classifier performance. These classifiers are used in a new classification approach to deal with class imbalance in Thin-Layer Chromatography (TLC) patterns, which is due to the huge difference between the number of normal and pathological cases, as a consequence of the rarity of Lysosomal Storage Disorders (LSD) diseases. The classification is performed in two decision stages, both implemented using optimized one-class classifiers: the first stage aims at recognizing most of the normal samples; the outliers of this first decision level are presented to the second stage, which is a multiclassifier prepared to deal with both pathological and normal patterns. The results that were obtained proved that the proposed methodology is able to overcome some of the difficulties associated with the choice of the rejection rate of one-class classifiers, and generally contribute to the minimization of the imbalance problem in TLC pattern classification. {\textcopyright} 2009 Springer Berlin Heidelberg.},
	keywords = {Chromatographic analysis, Class imbalance, Classification approach, Classifier performance, Classifiers, Decision levels, Image analysis, Imbalance problem, Learning systems, Lysosomal storage disorder, Multi-classifier, One-class classifier, Optimization, Pathological case, Pattern classification, Pattern recognition, Rejection rates, Thin layer chromatography},
	isbn = {03029743 (ISSN); 3642021719 (ISBN); 9783642021718 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-68749107350\&partnerID=40\&md5=17cc4075de5306819dcd1bfc005171d6},
	author = {Sousa, A.V. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2312,
	title = {Contextual classification of image patches with latent aspect models},
	journal = {Eurasip Journal on Image and Video ProcessingEurasip J. Image Video Process.},
	volume = {2009},
	year = {2009},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusArt. No.: 602920doi: 10.1155/2009/602920Language of Original Document: EnglishCorrespondence Address: Monay, F.; Idiap Research Institute Martigny, 1920 Martigny, Switzerland; email: florent.monay@idiap.chReferences: Kumar, S., Hebert, M., Discriminative random fields: A discriminative framework for contextual interaction in classification, 2, pp. 1150-1157. , Proceedings of the IEEE International Conference on Computer Vision (ICCV 03) October 2003 Nice, France;Lazebnik, S., Schmid, C., Ponce, J., Affine-invariant local descriptors and neighborhood statistics for texture recognition, 1, pp. 649-655. , Proceedings of the IEEE International Conference on Computer Vision (ICCV 03) October 2003 Nice, France; 
Vogel, J., Schiele, B., Natural scene retrieval based on a semantic modeling step, 3115, pp. 207-215. , Proceedings of the 3rd International Conference on Image and Video Retrieval (CIVR 04) July 2004 Dublin, Ireland Lecture Notes in Computer Science; 
Shotton, J., Winn, J., Rother, C., Criminisi, A., TextonBoost: Joint appearance, shape and context modeling for multi-class object recognition and segmentation, 3951, pp. 1-15. , Proceedings of the 9th European Conference on Computer Vision (ECCV 06) May 2006 Graz, Austria Lecture Notes in Computer Science; 
Lowe, D.G., Distinctive image features from scale-invariant keypoints (2004) International Journal of Computer Vision, 60 (2), pp. 91-110; 
Dorko, G., Schmid, C., Selection of scale-invariant parts for object class recognition, 1, pp. 634-640. , Proceedings of the 9th IEEE International Conference on Computer Vision (ICCV 03) October 2003 Nice, France; 
Fei-Fei, L., Perona, P., A Bayesian hierarchical model for learning natural scene categories, 2, pp. 524-531. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 05) June 2005 San Diego, Calif, USA; 
Quelhas, P., Monay, F., Odobez, J.-M., Gatica-Perez, D., Tuytelaars, T., Van Gool, L., Modeling scenes with local descriptors and latent aspects, 1, pp. 883-890. , Proceedings of the IEEE International Conference on Computer Vision (ICCV 05) October 2005 Beijing, China; 
Sivic, J., Russell, B.C., Efros, A.A., Zisserman, A., Freeman, W.T., Discovering object categories in image collections, , Proceedings of the 10th IEEE International Conference on Computer Vision (ICCV 05) October 2005 Beijing, China; 
Li, S.Z., (1995) Markov Random Field Modeling in Computer Vision, , New York, NY, USA Springer; 
Kumar, S., Hebert, M., Man-made structure detection in natural images using a causal multiscale random field, 1, pp. 119-126. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 03) June 2003 Madison, Wis, USA; 
Murphy, K., Torralba, A., Freeman, W., Using the forest to see the trees: A graphical model relating features, objects and scenes, , Proceedings of the Neural Information Processing Systems December 2003 Vancouver, Canada; 
Geman, S., Geman, D., Stochastic relaxation, Gibbs distributions, and the Bayesian restoration of images (1984) IEEE Transactions on Pattern Analysis and Machine Intelligence, 6 (6), pp. 721-741; 
Quelhas, P., Monay, F., Odobez, J.-M., Gatica-Perez, D., Tuytelaars, T., A thousand words in a scene (2007) IEEE Transactions on Pattern Analysis and Machine Intelligence, 29 (9), pp. 1575-1589; 
Quelhas, P., Odobez, J.-M., Multi-level local descriptor quantization for bag-of-visterms image representation, pp. 242-249. , Proceedings of the 6th ACM International Conference on Image and Video Retrieval (CIVR 07) July 2007 Amsterdam, The Netherlands; 
Csurka, G., Dance, C., Fan, L., Willamowski, J., Bray, C., Visual categorization with bags of keypoints, pp. 59-74. , Proceedings of the European Conference on Computer Vision (ECCV 04) May 2004 Prague, Czech Republic; 
Marszaek, M., Schmid, C., Spatial weighting for bag-of-features, 2, pp. 2118-2125. , Proceedings of IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 06) June 2006 New York, NY, USA; 
Wang, G., Zhang, Y., Fei-Fei, L., Using dependent regions for object categorization in a generative framework, 2, pp. 1597-1604. , Proceedings of IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 06) June 2006 New York, NY, USA; 
Sivic, J., Zisserman, A., Video Google: A text retrieval approach to object matching in videos, 2, pp. 1470-1477. , Proceedings of the 9th IEEE International Conference on Computer Vision (ICCV 03) October 2003 Nice, France; 
Willamowski, J., Arregui, D., Csurka, G., Dance, C.R., Fan, L., Categorizing nine visual classes using local appearance descriptors, , Proceedings of the Workshop on Learning for Adaptable Visual Systems (LAVS), in Conjunction with the International Conference on Pattern Recognition (ICPR 04) August 2004 Cambridge, UK; 
Bosch, A., Zisserman, A., Muoz, X., Scene classification via pLSA, 3954, pp. 517-530. , Proceedings of the 9th European Conference on Computer Vision (ECCV 06) May 2006 Graz, Austria Lecture Notes in Computer Science; 
Hofmann, T., Unsupervised learning by probabilistic latent semantic analysis (2001) Machine Learning, 42 (12), pp. 177-196; 
Blei, D.M., Ng, A.Y., Jordan, M.I., Latent Dirichlet allocation (2003) Journal of Machine Learning Research, 3 (45), pp. 993-1022; 
Pal, N.R., Pal, S.K., A review on image segmentation techniques (1993) Pattern Recognition, 26 (9), pp. 1277-1294; 
Carson, C., Thomas, M., Belongie, S., Hellerstein, J.M., Malik, J., Blob-world: A system for region-based image indexing and retrieval, pp. 509-516. , Proceedings of the 3rd International Conference on Visual Information Systems (VISUAL 99) June 1999 Amsterdam, The Netherlands Springer; 
Duygulu, P., Barnard, K., De Freitas, J.F.G., Forsyth, D.A., Object recognition as machine translation: Learning a lexicon for a fixed image vocabulary, 2353, pp. 97-112. , Proceedings of the 7th European Conference on Computer Vision (ECCV 02) May 2002 Copenhagen, Denmark Lecture Notes in Computer Science; 
Vogel, J., Schiele, B., A semantic typicality measure for natural scene categorization, 3175, pp. 195-203. , Proceedings of the 26th DAGM Pattern Recognition Symposium August-September 2004 Tbingen, Germany Lecture Notes in Computer Science; 
Verbeek, J.J., Triggs, B., Region classification with Markov field aspect models, pp. 1-8. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 07) June 2007 Minneapolis, Minn, USA; 
Shi, J., Malik, J., Normalized cuts and image segmentation, pp. 731-737. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 97) June 1997 San Juan, Puerto Rico, USA; 
Kumar, M.P., Torr, P.H.S., Zisserman, A., Obj cut, 1, pp. 18-25. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 05) June 2005 San Diego, Calif, USA; 
Leibe, B., Leonardis, A., Schiele, B., Combined object categorization and segmentation with an implicit shape model, pp. 17-32. , Proceedings of the 8th European Conference on Computer Vision (ECCV 04) May 2004 Prague, Czech Republic; 
Russell, B.C., Freeman, W.T., Efros, A.A., Sivic, J., Zisserman, A., Using multiple segmentations to discover objects and their extent in image collections, 2, pp. 1605-1612. , Proceedings of IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 06) June 2006 New York, NY, USA; 
Monay, F., Quelhas, P., Odobez, J.-M., Gatica-Perez, D., Integrating co-occurrence and spatial contexts on patch-based scene segmentation, p. 14. , Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition Workshop (CVPR 06) June 2006 New York, NY, USA; 
Cao, L., Fei-Fei, L., Spatially coherent latent topic model for concurrent object segmentation and classification, , Proceedings of the 11th IEEE International Conference on Computer Vision (ICCV 07) October 2007 Rio de Janeiro, Brazil; 
Liu, D., Chen, T., Background cutout with automatic object discovery, 4, pp. 345-348. , Proceedings of IEEE International Conference on Image Processing (ICIP 07) September 2007 San Antonio, Tex, USA; 
Tuytelaars, T., Van Gool, L., Content-based image retrieval based on local affinely invariant regions, 1614, pp. 493-500. , Proceedings of the 3rd International Conference on Visual Information and Information Systems June 1999 Amsterdam, The Netherlands Lecture Notes in Computer Science; 
Mikolajczyk, K., Schmid, C., A performance evaluation of local descriptors, 2, pp. 257-263. , Proceedings of the IEEE Computer Society Conference on Computer Vision and Pattern Recognition (CVPR 03) June 2003 Madison, Wis, USA; 
Mikolajczyk, K., Tuytelaars, T., Schmid, C., A comparison of affine region detectors (2005) International Journal of Computer Vision, 65 (12), pp. 43-72; 
Baeza-Yates, R., Ribeiro-Neto, B., (1999) Modern Information Retrieval, , New York, NY, USA ACM Press; 
Monay, F., Quelhas, P., Gatica-Perez, D., Odobez, J.-M., Constructing visual models with a latent space approach, 3940, pp. 115-126. , Proceedings of the Subspace, Latent Structure and Feature Selection, Statistical and Optimization, Perspectives Workshop (SLSFS 05) February 2006 Bohinj, Slovenia Lecture Notes in Computer Science; 
Fergus, R., Fei-Fei, L., Perona, P., Zisserman, A., Learning object categories from Googles image search, 2, pp. 1816-1823. , Proceedings of the 10th IEEE International Conference on Computer Vision (ICCV 05) October 2005 Beijing, China; 
Buntine, W.L., Variational extensions to em and multinomial PCA, 2430, pp. 23-34. , Proceedings of the 13th European Conference on Machine Learning August 2002 Helsinki, Finland Lecture Notes in Computer Science; 
Bishop, C., (1995) Neural Networks for Pattern Recognition, , Oxford, UK Oxford University
},
	month = {2009///},
	abstract = {We present a novel approach for contextual classification of image patches in complex visual scenes, based on the use of histograms of quantized features and probabilistic aspect models. Our approach uses context in two ways: (1) by using the fact that specific learned aspects correlate with the semantic classes, which resolves some cases of visual polysemy often present in patch-based representations, and (2) by formalizing the notion that scene context is image-specificwhat an individual patch represents depends on what the rest of the patches in the same image are. We demonstrate the validity of our approach on a man-made versus natural patch classification problem. Experiments on an image collection of complex scenes show that the proposed approach improves region discrimination, producing satisfactory results and outperforming two noncontextual methods. Furthermore, we also show that co-occurrence and traditional (Markov random field) spatial contextual information can be conveniently integrated for further improved patch classification.},
	isbn = {16875176 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-61449241990\&partnerID=40\&md5=b909ab8585061d7873ec0fe14a47dba8},
	author = {Monay, F. and Quelhas, P. and Odobez, J.-M. and Gatica-Perez, D.}
}
@article {2315,
	title = {Corrected and improved model for educational simulation of neonatal cardiovascular pathophysiology},
	journal = {Simulation in HealthcareSimul. Healthc.},
	volume = {4},
	year = {2009},
	note = {Cited By (since 1996): 4Export Date: 21 November 2012Source: Scopusdoi: 10.1097/SIH.0b013e31818b27a8Language of Original Document: EnglishCorrespondence Address: Zijlmans, M.; INEB-Instituto de Engenharia Biom{\'e}dica, Porto, PortugalReferences: S{\'a} Couto, C.D., Van Meurs, W.L., Goodwin, J.A., Andriessen, P., A model for educational simulation of neonatal cardiovascular pathophysiology (2006) Simul Healthc, 1, pp. 4-12;Goodwin, J.A., Van Meurs, W.L., S{\'a} Couto, C.D., Beneken, J.E.W., Graves, S.A., A model for educational simulation of infant cardiovascular physiology (2004) Anesth Analg, 99, pp. 1655-1664; 
Beneken, J.E.W., A mathematical approach to cardiovascular function (1965) The Uncontrolled Human System, , Thesis, University of Utrecht, The Netherlands; 
Andriessen, P., Bambang Oetomo, S., Peters, C., Vermeulen, B., Wijn, P.F.F., Blanco, C.E., Baroreceptor reflex sensitivity in human neonates: The effect of postmenstrual age (2005) J Physiol, 568, pp. 333-341; 
Segar, J.L., Roghair, R.D., Segar, E.M., Bailey, M.C., Scholz, T.D., Lamb, F.S., Early gestation dexamethasone alters baroreflex and vascular responses in newborn lambs before hypertension (2006) Am J Physiol Regul Integr Comp Physiol, 291, pp. R481-R488; 
Wallgren, G., Barr, M., Rudhe, U., Hemodynamic studies of induced acute hypo- and hypervolemia in the newborn infant (1964) Acta Paediatr, 53, pp. 1-12
},
	month = {2009///},
	pages = {49 - 53},
	abstract = {We identified errors in the software implementation of the mathematical model presented in: S{\'a} Couto CD, van Meurs WL, Goodwin JA, Andriessen P. A model for educational simulation of neonatal cardiovascular pathophysiology. Simul Healthcare 2006;1:4-12. Simulation results obtained with corrected code are presented for future reference. All but one of the simulation results do not differ by more than 9\% from the previously published results. The heart rate response to acute loss of 30\% of blood volume, simulated with corrected code is stronger than published target data. This modeling error was masked by errors in code implementation. We improved this response and the model by adjusting the gains and adding thresholds and saturations in the baroreflex model. General considerations on identification of model and code errors and model validity are presented. Copyright {\textcopyright} 2009 Society for Simulation in Healthcare.},
	keywords = {aorta coarctation, article, Baroreflex, bleeding, blood pressure, blood volume, Cardiac Output, cardiovascular disease, Cardiovascular Diseases, Cardiovascular physiology, computer program, Computer simulation, Congenital heart defect, congenital heart malformation, diastolic blood pressure, Education, Medical, Erratum, Fallot tetralogy, great vessels transposition, heart output, Heart rate, heart right ventricle pressure, human, Humans, Infant, Newborn, lung pressure, mathematical model, mean arterial pressure, medical education, medical error, methodology, Neonate, Neonatology, newborn, patent ductus arteriosus, pathophysiology, physiology, pressoreceptor reflex, priority journal, simulation, Software Design, systolic blood pressure, Validation, validity, vascular resistance, Verification, vital sign},
	isbn = {15592332 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-62849083921\&partnerID=40\&md5=8cf0edc0b85c1bd093cd9b3a8c70de77},
	author = {Zijlmans, M. and S{\'a}-Couto, C.D. and van Meurs, W.L. and Goodwin, J.A. and Andriessen, P.}
}
@article {2323,
	title = {The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers},
	journal = {BiomaterialsBiomaterials},
	volume = {30},
	year = {2009},
	note = {Cited By (since 1996): 41Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2008.09.048PubMed ID: 18952279Language of Original Document: EnglishCorrespondence Address: Barrias, C.C.; INEB - Instituto de Engenharia Biom{\'e}dica, Divis{\~a}o de Biomateriais, Universidade do Porto, R. Campo Alegre 823, 4150-180 Porto, Portugal; email: ccbarrias@ineb.up.ptChemicals/CAS: fibronectin, 86088-83-7; gold, 7440-57-5; thiol derivative, 13940-21-1; Biocompatible Materials; Fibronectins; VitronectinReferences: Wilson, C.J., Clegg, R.E., Leavesley, D.I., Pearcy, M.J., Mediation of biomaterial-cell interactions by adsorbed proteins: a review (2005) Tissue Eng, 11, pp. 1-18;Garc{\'\i}a, A.J., Vega, M.D., Boettiger, D., Modulation of cell proliferation and differentiation through substrate-dependent changes in fibronectin conformation (1999) Mol Biol Cell, 10, pp. 785-798; 
Keselowsky, B.G., Collard, D.M., Garc{\'\i}a, A.J., Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion (2003) J Biomed Mater Res A, 66, pp. 247-259; 
Underwood, P.A., Bennett, F.A., A comparison of the biological activities of the cell-adhesive proteins vitronectin and fibronectin (1989) J Cell Sci, 93, pp. 641-649; 
Steele, J.G., Johnson, G., Underwood, P.A., Role of serum vitronectin and fibronectin in adhesion of fibroblasts following seeding onto tissue culture polystyrene (1992) J~Biomed Mater Res, 26, pp. 861-884; 
Steele, J.G., Dalton, B.A., Johnson, G., Underwood, P.A., Polystyrene chemistry affects vitronectin activity: an explanation for cell attachment to tissue culture polystyrene but not to unmodified polystyrene (1993) J Biomed Mater Res, 27, pp. 927-940; 
Underwood, P.A., Steele, J.G., Dalton, B.A., Effects of polystyrene surface chemistry on the biological activity of solid phase fibronectin and vitronectin, analysed with monoclonal antibodies (1993) J Cell Sci, 104, pp. 793-803; 
Suzuki, S., Oldberg, A., Hayman, E.G., Pierschbacher, M.D., Ruoslahti, E., Complete amino acid sequence of human vitronectin deduced from cDNA. Similarity of cell attachment sites in vitronectin and fibronectin (1985) EMBO J, 4, pp. 2519-2524; 
Lee, M.H., Ducheyne, P., Lynch, L., Boettiger, D., Composto, R.J., Effect of biomaterial surface properties on fibronectin-alpha5beta1 integrin interaction and cellular attachment (2006) Biomaterials, 27, pp. 1907-1916; 
Grinnell, F., Feld, M.K., Fibronectin adsorption on hydrophilic and hydrophobic surfaces detected by antibody binding and analyzed during cell adhesion in serum-containing medium (1982) J Biol Chem, 257, pp. 4888-4893; 
Sousa, S.R., Lamghari, M., Sampaio, P., Moradas-Ferreira, P., Barbosa, M.A., Osteoblast adhesion and morphology on TiO 2 depends on the competitive preadsorption of albumin and fibronectin (2008) J Biomed Mater Res A, 84, pp. 281-290; 
Fabrizius-Homan, D.J., Cooper, S.L., Competitive adsorption of vitronectin with albumin, fibrinogen, and fibronectin on polymeric biomaterials (1991) J Biomed Mater Res, 25, pp. 953-971; 
Bale, M.D., Wohlfahrt, L.A., Mosher, D.F., Tomasini, B., Sutton, R.C., Identification of vitronectin as a major plasma protein adsorbed on polymer surfaces of different copolymer composition (1989) Blood, 74, pp. 2698-2706; 
Thomas, C.H., McFarland, C.D., Jenkins, M.L., Rezania, A., Steele, J.G., Healy, K.E., The role of vitronectin in the attachment and spatial distribution of bone-derived cells on materials with patterned surface chemistry (1997) J Biomed Mater Res, 37, pp. 81-93; 
McFarland, C.D., Thomas, C.H., DeFilippis, C., Steele, J.G., Healy, K.E., Protein adsorption and cell attachment to patterned surfaces (2000) J Biomed Mater Res, 49, pp. 200-210; 
Love, J.C., Estroff, L.A., Kriebel, J.K., Nuzzo, R.G., Whitesides, G.M., Self-assembled monolayers of thiolates on metals as a form of nanotechnology (2005) Chem Rev, 105, pp. 1103-1169; 
Mrksich, M., Whitesides, G.M., Using self-assembled monolayers to understand the interactions of man-made surfaces with proteins and cells (1996) Annu Rev Biophys Biomol Struct, 25, pp. 55-78; 
Bain, C.D., Troughton, E.B., Tao, Y.T., Evall, J., Whitesides, G.M., Nuzzo, R.G., Formation of monolayer films by the spontaneous assembly of organic thiols from solution onto gold (1989) J Am Chem Soc, 111, pp. 321-335; 
Faucheux, N., Schweiss, R., L{\"u}tzow, K., Werner, C., Groth, T., Self-assembled monolayers with different terminating groups as model substrates for cell adhesion studies (2004) Biomaterials, 25, pp. 2721-2730; 
Bernards, M.T., Jiang, S., pH-induced conformation changes of adsorbed vitronectin maximize its bovine aortic endothelial cell binding ability (2008) J Biomed Mater Res A; 
Martins, M.C., Ratner, B.D., Barbosa, M.A., Protein adsorption on mixtures of hydroxyl- and methyl-terminated alkanethiols self-assembled monolayers (2003) J~Biomed Mater Res A, 67, pp. 158-171; 
Rodrigues, S.N., Gon{\c c}alves, I.C., Martins, M.C., Barbosa, M.A., Ratner, B.D., Fibrinogen adsorption, platelet adhesion and activation on mixed hydroxyl-/methyl-terminated self-assembled monolayers (2006) Biomaterials, 27, pp. 5357-5367; 
Chamberlain, J., Yamagami, T., Colletti, E., Theise, N.D., Desai, J., Frias, A., Efficient generation of human hepatocytes by the intrahepatic delivery of clonal human mesenchymal stem cells in fetal sheep (2007) Hepatology, 46, pp. 1935-1945; 
(1993) Iodine-125. A guide to radioiodination techniques, , Amersham Life Science, Little Chalfort; 
Horbett, T.A., Techniques for protein adsorption studies (1986) Techniques of biocompatibility tests, pp. 183-214. , Williams D.F. (Ed), CRC Press Inc., Harcover; 
Arima, Y., Iwata, H., Effect of wettability and surface functional groups on protein adsorption and cell adhesion using well-defined mixed self-assembled monolayers (2007) Biomaterials, 28, pp. 3074-3082; 
Capadona, J.R., Collard, D.M., Garcia, A.J., Fibronectin adsorption and cell adhesion to mixed monolayers of tri(ethylene glycol)- and methyl-terminated alkanethiols (2003) Langmuir, 19, pp. 1847-1852; 
Tidwell, C.D., Ertel, S.I., Ratner, B.D., Tarasevich, B.J., Atre, S., Allara, D.L., Endothelial cell growth and protein adsorption on terminally functionalized, self-assembled monolayers of alkanethiolates on gold (1997) Langmuir, 13, pp. 3404-3413; 
Jenney, C.R., Anderson, J.M., Adsorbed serum proteins responsible for surface dependent human macrophage behavior (2000) J Biomed Mater Res, 49, pp. 435-447; 
Wertz, C.F., Santore, M.M., Fibrinogen adsorption on hydrophilic and hydrophobic surfaces: geometrical and energetic aspects of interfacial relaxations (2002) Langmuir, 18, pp. 706-715; 
Pitt, W.G., Fabrizius-Homan, D.J., Mosher, D.F., Cooper, S.L., Vitronectin adsorption on polystyrene and oxidized polystyrene (1989) J Colloid Interface Sci, 129, pp. 231-239; 
Seiffert, D., Smith, J.W., The cell adhesion domain in plasma vitronectin is cryptic (1997) J Biol Chem, 272, pp. 13705-13710
},
	month = {2009///},
	pages = {307 - 316},
	abstract = {The objective of this study was to compare the biological effects of two key cell-adhesive proteins, fibronectin (FN) and vitronectin (VN), upon adsorption onto molecularly-designed model surfaces. Single-component and mixed self-assembled monolayers (SAMs) of alkanethiols on gold with OH and CH                        3 terminal groups were prepared at 100\%, 65\%, 36\% and 0\% of OH at the surface, to generate a range of surfaces with a simple chemistry and a wettability gradient. FN and VN were adsorbed under non-competitive (single-protein solutions) and competitive (multi-protein solutions) conditions, and compared at different levels: adsorbed amount (radiolabelling), elution, functional presentation of cell-binding domains (ELISA), and role in mediating cell adhesion (antibody-based assay). The observed trends were related to mesenchymal stem cell response in terms of adhesion and overall cell morphology. Under non-competitive conditions, adsorption of both proteins increased with surface hydrophobicity. The presence of competitive proteins significantly decreased the adsorbed amounts, although both proteins were still detected in all SAMs. Adsorption of FN followed a trend similar to that of non-competitive conditions, while adsorption of VN was higher on 100\%OH-SAMs. Concerning elution, retention of adsorbed VN was always higher than that of FN. For both proteins, functional presentation of cell-binding domains was more effective on the more hydrophilic 100\%OH-SAMs. This fact, coupled to the ability of this type of SAMs to selectively recruit and retain VN in the presence of competitive serum proteins, seems to correlate with the better cell response observed on these surfaces, as compared with hydrophobic 0\%OH(100\%CH
                        3)-SAMs. {\textcopyright} 2008 Elsevier Ltd. All rights reserved.
},
	keywords = {Adhesion, Adhesiveness, Adsorption, alkane, Animals, article, Biocompatible Materials, Biological Assay, bone marrow, Cattle, cell adhesion, Cell culture, Cell Movement, Cells, Cells, Cultured, cytology, cytoskeleton, Electric currents, Electron energy levels, enzyme linked immunosorbent assay, Fibronectin, Fibronectins, Focal adhesions, Functional groups, Functionalization, gold, human, human cell, Humans, hydrophilicity, hydrophobicity, Hydroxylation, isotope labeling, Mesenchymal stem cell, Mesenchymal Stem Cells, Microscopy, Confocal, Missile bases, Molecular interactions, monoclonal antibody, priority journal, Protein adsorption, protein analysis, protein binding, Protein Structure, Tertiary, Proteins, Self assembled monolayers, Self-assembled monolayers, stem cell, Stem cells, Surface Properties, thiol derivative, Vitronectin},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-55249093830\&partnerID=40\&md5=132fa4efe300c4f76764942bfe3bce30},
	author = {Barrias, C.C. and Martins, M.C.L. and Almeida-Porada, G. and Barbosa, M.A. and Granja, P.L.}
}
@article {2321,
	title = {Cytotoxicity evaluation of nanocrystalline diamond coatings by fibroblast cell cultures},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {5},
	year = {2009},
	note = {Cited By (since 1996): 8Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2008.08.015PubMed ID: 18819854Language of Original Document: EnglishCorrespondence Address: Amaral, M.; CICECO, Department of Ceramics and Glass Engineering, University of Aveiro, 3810-193 Aveiro, Portugal; email: margarida.amaral@ua.ptChemicals/CAS: 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, 298-93-1; diamond, 7782-40-3; polystyrene, 9003-53-6; silicon nitride, 12033-89-5; Culture Media; Diamond, 7782-40-3References: Griffin, J., Ray, P.C., Role of inert gas in the low-temperature nano-diamond chemical vapour deposition process (2006) Nanotechnology, 17, pp. 1225-1229;Mitura, S., Mitura, K., Niedzielski, P., Louda, P., Danilenko, V., Nanocrystalline diamond, its synthesis, properties and applications (2006) J Achievements Mater Manuf Eng, 16, pp. 9-16; 
Okr{\'o}j, W., Kami{\'n}ska, M., Klimek, L., Szyma{\'n}ski, W., Walkowiak, B., Blood platelets in contact with nanocrystalline diamond surfaces (2006) Diamond Relat Mater, 15, pp. 1535-1539; 
Xiao, X., Wang, J., Liu, C., Carlisle, J.A., Mech, B., Greenberg, R., In vitro and in vivo evaluation of ultrananocrystalline diamond for coating of implantable retinal microchips (2006) J Biomed Mater Res B: Appl Biomater, 77 B, pp. 273-281; 
Tang, L., Tsai, C., Gerberich, W.W., Kruckeberg, L., Kania, D.R., Biocompatibility of chemical-vapour-deposited diamond (1995) Biomaterials, 16, pp. 483-488; 
Steinmueller-Nethl, D., Kloss, F.R., Haq, M.N.U., Rainer, M., Larsson, K., Linsmeier, C., Strong binding of bioactive BMP-2 to nanocrystalline diamond by physisorption (2006) Biomaterials, 27, pp. 4547-4556; 
Wenmackers, S., Christiaens, P., Daenen, M., Haenen, K., Nesl{\'a}dek, M., Ven, M., DNA attachment to nanocrystalline diamond films (2005) Phys Status Solidi, 202, pp. 2212-2216; 
Retama, J.R., Hernando, J., Lopez-Ruiz, B., H{\"a}rtl, A., Steinm{\"u}ller, D., Stutzmann, M., Synthetic nanocrystalline diamond as a third-generation biosensor support (2006) Langmuir, 22, pp. 5837-5842; 
Siew, P.S., Loh, K.P., Poh, W.C., Zhang, H., Biosensing properties of nanocrystalline diamond film grown on polycrystalline diamond electrodes (2005) Diamond Relat Mater, 14, pp. 426-431; 
Jakubowski, W., Bartosz, G., Niedzielski, P., Szymanski, W., Walkowiak, B., Nanocrystalline diamond surface is resistant to bacterial colonization (2004) Diamond Relat Mater, 13, pp. 1761-1763; 
Trava-Airoldi, V.J., Corat, E.J., Leite, N.F., Nono, M., Ferreira, N.G., Baranauskas, V., CVD diamond burrs-development and applications (1996) Diamond Relat Mater, 5, pp. 857-860; 
Amar, M., Ahmed, W., Sein, H., Jones, A.N., Rego, C., Chemical vapour deposition of diamond coatings onto molybdenum dental tools (2003) J Phys Condens Matter, 15, pp. S2977-S2982; 
Tetsumura, A., Honda, E., Sasaki, T., Kino, K., Metallic residues as a source of artifacts in magnetic resonance imaging of the temporomandibular joint (1999) Dentomaxillofac Radiol, 28, pp. 186-190; 
Carvalho, C.A., Fagundes, T.C., Barata, T.E., Trava-Airoldi, V.J., Navarro, M.L., The use of CVD diamond burrs for ultraconservative cavity preparations: a report of two cases (2007) J Esthet Restor Dent, 19, pp. 19-29; 
Mitura, K., Niedzielski, P., Grzegorz, B., Moll, J., Walkowiak, B., Pawlowska, Z., Interactions between carbon coatings and tissue (2006) Surf Coat Technol, 201, pp. 2117-2123; 
Fries, M.D., Vohra, Y.K., Microcrystalline and nanocrystalline diamond film deposition on cobalt chrome alloy (1999) Thin films-stresses and mechanical properties VIII, , Vinci R., Kraft O., Moody N., Besser P., and Shaffer II E. (Eds), MRS Publications, Boston, MA; 
Fries, M.D., Vohra, Y.K., Properties of nanocrystalline thin films grown by MPCVD for biomedical implant purposes (2004) Diamond Relat Mater, 13, pp. 1740-1743; 
Papo, M.J., Catledge, S.A., Vohra, Y.K., Mechanical wear behavior of nanocrystalline and multilayer diamond coatings on temporomandibular joint implants (2004) J Mater Sci: Mater Med, 15, pp. 773-777; 
Sun, F.H., Zhang, Z.M., Ma, Y.P., Chen, M., Synthesis of nanocrystalline diamond films on smooth WC-Co cemented carbide substrates (2007) Int J Abrasive Technol, 1, pp. 161-172; 
Belmonte, M., Fernandes, A.J.S., Costa, F.M., Oliveira, F.J., Silva, R.F., Adhesion behaviour assessment on diamond coated silicon nitride by acoustic emission (2003) Diamond Relat Mater, 12, pp. 733-737; 
Petzow, G., Herrmann, M., Silicon nitride ceramics (2002) Struct Bond, 102, pp. 47-167; 
Amaral, M., Costa, M.A., Lopes, M.A., Silva, R.F., Santos, J.D., Fernandes, M.H., Si 3N 4-bioglass composites stimulate the proliferation of MG63 osteoblast-like cells and osteogenic differentiation of human bone marrow cells (2002) Biomaterials, 23, pp. 4897-4906; 
Kue, R., Sahrabi, A., Nagle, D., Frondonza, C., Hungerford, D., Enhanced proliferation and osteocalcin production by human osteoblast-like cells on silicon nitride ceramic discs (1999) Biomaterials, 20, pp. 1195-1201; 
Popov, C., Kulisch, W., Jelinek, M., Bock, A., Strnad, J., Nanocrystalline diamond/amorphous carbon composite films for applications in tribology, optics and biomedicine (2006) Thin Solid Films, 494, pp. 92-97; 
Chong, K.F., Loh, K.P., Vedula, S.R., Lim, C.T., Sternschulte, H., Steinm{\"u}ller, D., Cell adhesion properties on photochemically functionalized diamond (2007) Langmuir, 23, pp. 5615-5621; 
Bajaj, P., Akin, D., Gupta, A., Sherman, D., Shi, B., Auciello, O., Ultra-nanocrystalline diamond film as an optimal cell interface for biomedical applications (2007) Biomed Microdevices, , [Epub ahead of print]; 
Arnold, L.F., Baram, B., In vitro culture of periodontal ligament cells (1972) J Dent Res, 51, pp. 953-959; 
Piche, J.E., Carnes Jr., D.L., Graves, D.T., Initial characterization of cells derived from human periodontia (1989) J Dent Res, 68, pp. 761-767; 
Trigo Cabral, C., Costa, M.A., Fernandes, M.H., In vitro models of periodontal cells: a comparative study of long-term gingival, periodontal ligament and alveolar bone cell cultures in the presence of β-glycerophosphate and dexamethasone (2007) J Mater Sci: Mater Med, 18, pp. 1079-1088; 
Bajpai, P.K., Billote, W.G., Ceramic biomaterials (1995) The biomedical engineering handbook, pp. 552-580. , Bronzino J.D. (Ed), CRC Press, Boca Raton, FL; 
Nishitani-Gamo, M., Ando, T., Watanabe, K., Sekita, M., Dennig, P.A., Yamamoto, K., Interfacial structures of oriented diamond on Si (1 0 0) characterized by confocal Raman spectroscopy (1997) Diamond Relat Mater, 6, pp. 1036-1040; 
Ferrari, A.C., Robertson, J., Origin of the 1150 cm -1 Raman mode in nanocrystalline diamond (2001) Phys Rev B, 63, pp. 121405-1-121405-4; 
Pfeiffer, R., Kuzmany, H., Knoll, P., Bokova, S., Salk, N., G{\"u}nther, B., Evidence for trans-polyacetylene in nano-crystalline diamond films (2003) Diamond Relat Mater, 12, pp. 268-271; 
Zimmermann, T., Janischowsky, K., Denisenko, A., Guill{\'e}n, F.J.H., Kubovic, M., Gruen, D.M., Nanocrystalline diamond pn-structure grown by hot-filament CVD (2006) Diamond Relat Mater, 15, pp. 203-206; 
Krauss, A.R., Auciello, O., Gruen, D.M., Jayatissa, A., Sumant, A., Tucek, J., Ultrananocrystalline diamond thin films for MEMS and moving mechanical assembly devices (2001) Diamond Relat Mater, 10, pp. 1952-1961; 
Amaral, M., Fernandes, A.J.S., Vila, M., Oliveira, F.J., Silva, R.F., Growth rate improvements in the hot-filament CVD deposition of nanocrystalline diamond (2006) Diamond Relat Mater, 15, pp. 1822-1827; 
Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P., The cytoskeleton (2002) Molecular biology of the cell. 4th ed., pp. 907-972. , Alberts B., Johnson A., Lewis J., Raff M., Roberts K., and Walter P. (Eds), Garland Publishing, New York; 
Stamenovic, D., Effects of cytoskeletal prestress on cell rheological behaviour (2005) Acta Biomater, 1, pp. 255-262; 
Freshney, T.I., Biology of cultured cells (2000) Culture of animal cells. A manual of basic technique, pp. 9-18. , Wiley-Liss, New York; 
Garguilo, J.M., Davis, B.A., Buddie, M., K{\"o}ck, F.A.M., Nemanich, R.J., Fibrinogen adsorption onto microwave plasma chemical vapor deposited diamond films (2004) Diamond Relat Mater, 13, pp. 595-599; 
Ariano, P., Baldellia, P., Carbonea, E., Gilardino, A., Giudicea, A.L., Lovisolo, D., Cellular adhesion and neuronal excitability on functionalised diamond surfaces (2005) Diamond Relat Mater, 14, pp. 669-674; 
Heinrich, G., Grijgler, T., Rosiwal, S.M., Singer, R.F., CVD diamond coated titanium alloys for biomedical and aerospace applications (1997) Surf Coat Technol, 94-95, pp. 514-520; 
M{\"u}ller, R., Adamschik, M., Steidl, D., Kohn, E., Thamasett, S., Stiller, S., Application of CVD-diamond for catheter ablation in the heart (2004) Diamond Relat Mater, 13, pp. 1080-1083; 
Nordsletten, L., Hogasen, A.K.M., Konttinen, Y.T., Santavirta, S., Aspenberg, P., Aasen, A.O., Human monocytes stimulation by particles of hydroxyapatite, silicon carbide and diamond: in vitro studies of new prosthesis coatings (1996) Biomaterials, 17, pp. 1521-1527; 
Aspenberg, P., Antilla, A., Konttinen, Y.T., Lappalainen, R., Goodman, S.B., Nordsletten, L., Benign response to particles of diamond and SiC: bone chamber studies of new joint replacement coating materials in rabbits (1996) Biomaterials, 17, pp. 807-812; 
Specht, C.G., Williams, O.A., Jackman, R.B., Schoepfer, R., Ordered growth of neurons on diamond (2004) Biomaterials, 25, pp. 4073-4078; 
Schrand, A.M., Huang, H., Carlson, C., Schlager, J.J., Omacr Sawa, E., Hussain, S.M., Are diamond nanoparticles cytotoxic? (2007) J Phys Chem B, 111, pp. 2-7; 
International Organization for Standardization, ISO 10993-5: biological evaluation of medical devices. Part 5. Tests for cytotoxicity: in vitro methods. Geneva; 1992International Organization for Standardization, ISO 7405: dentistry-preclinical evaluation of biocompatibility of medical devices used in dentistry-test methods for dental materials. Geneva; 1997Kaplan, F.S., Hayes, W.C., Keaveny, T.M., Boskey, A., Einhorn, T.A., Iannotti, J.P., Form and function of bone (1994) Orthopedic basic science, pp. 127-185. , Simon S.P. (Ed), American Academy of Orthopedic Surgeons, Columbus, OH; 
Webster, T.J., Proteins: structure and interactions patterns to solid surfaces (2004) Encyclopedia of nanoscience and nanotechnology, pp. 3079-3095. , Schwarz J.A., Contescu C., and Putyera K. (Eds), Marcel Dekker, New York; 
Webster, T.J., Enhanced functions of osteoblasts on nanophase ceramics (2000) Biomaterials, 21, pp. 1803-1810; 
Webster, T.J., Ergun, C., Doremus, R.H., Siegel, R.W., Bizios, R., Specific proteins mediate enhanced osteoblast adhesion on nanophase ceramics (2000) J Biomed Mater Res, 51, pp. 475-483; 
Shane, A.C., Marc, D.F., Yogesh, K.V., William, R.L., Jack, E.L., Shanna, W., Nanostructured ceramics for biomedical implants (2002) J Nanosci Nanotechnol, 2, pp. 1-20; 
Price, R.L., Haberstroh, K.M., Webster, T.J., Enhanced functions of osteoblasts on nanostructured surfaces of carbon and alumina (2003) Med Biol Eng Comput, 41, pp. 372-375; 
Webster, T.J., Siegel, R.W., Bizios, R., Osteoblast adhesion on nanophase ceramics (1999) Biomaterials, 20, pp. 1221-1227; 
Perla, V., Webster, T.J., Better osteoblast adhesion on nanoparticulate selenium-A promising orthopedic implant material (2005) J Biomed Mater Res, 75 A, pp. 356-364
},
	month = {2009///},
	pages = {755 - 763},
	abstract = {The cytotoxicity profile of nanocrystalline diamond (NCD) coatings on a Si                        3N
                        4 ceramic was investigated. This material is envisaged to have biomedical dental applications such as burrs and surgical instruments. Two fibroblast cell culture systems were used to address the cytotoxicity of NCD-coated samples: L929 cells (a mouse permanent cell line) and human gingival fibroblasts. Cell behavior was evaluated in terms of cell adhesion, cell viability/proliferation (mitochondrial function, MTT assay) and the pattern of cell growth. Fibroblast cell behavior on standard polystyrene culture plates was used as control, as Si
                        3N
                        4 substrates have previously been shown to be biocompatible. NCD coatings provided a suitable surface for cell attachment, spreading and proliferation. Human gingival cells showed a homogeneous cytoplasm spreading, a flattened elongated morphology and a typical parallel alignment on confluent cultures. In comparison, L929 cells denoted a lower cytoplasm expansion, a heterogeneous spreading but a higher proliferation rate. For both cells, after few days, the NCD coating was completely covered with continuous cell layers. As compared to standard polystyrene culture plates, no deleterious or cytotoxic responses were observed with L929 and human fibroblast cell cultures, and in both a slight enhancement in cell proliferation was observed. In addition, the seeded NCD film allowed reproduction of the typical features of the two cell culture systems tested, further suggesting the lack of cytotoxicity of this coating. {\textcopyright} 2008 Acta Materialia Inc.
},
	keywords = {3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, animal cell, Animals, article, Biocompatibility, Biomedical dental applications, biomedicine, cell adhesion, Cell Division, cell function, cell growth, Cell Line, cell proliferation, cell spreading, cell structure, cell surface, cell survival, cell viability, ceramics, controlled study, Culture Media, cytoplasm, Cytotoxicity, dentistry, diamond, Fibroblast cell cultures, fibroblast culture, Fibroblasts, gingiva, human, human cell, Humans, material coating, Mice, Microscopy, Electron, Scanning, mitochondrial respiration, mouse, nanocrystal, Nanocrystalline diamond coating, Nanostructures, nonhuman, polystyrene, priority journal, Silicon nitride, Surgical instrument},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-58049191417\&partnerID=40\&md5=f75616146e61ce84434c5481a2b8eec3},
	author = {Amaral, M. and Gomes, P.S. and Lopes, M.A. and Santos, J.D. and Silva, R.F. and Fernandes, M.H.}
}
@article {2352,
	title = {Cell-induced response by tetracyclines on human bone marrow colonized hydroxyapatite and Bonelike                    {\textregistered}
                
},
	journal = {Acta BiomaterialiaActa Biomater.},
	volume = {4},
	year = {2008},
	note = {Cited By (since 1996): 9Export Date: 21 November 2012Source: Scopusdoi: 10.1016/j.actbio.2007.12.006PubMed ID: 18291737Language of Original Document: EnglishCorrespondence Address: Fernandes, M.H.; Laborat{\'o}rio de Farmacologia e Biocompatibilidade Celular, Faculdade de Medicina Dent{\'a}ria, Universidade do Porto (FMDUP), Rua Dr. Manuel Pereira da Silva, s/n, 4200-392 Porto, Portugal; email: mhrf@portugalmail.ptChemicals/CAS: calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; doxycycline, 10592-13-9, 17086-28-1, 564-25-0; hydroxyapatite, 1306-06-5, 51198-94-8; minocycline, 10118-90-8, 11006-27-2, 13614-98-7; Alkaline Phosphatase, EC 3.1.3.1; Bone Substitutes; Calcium, 7440-70-2; Culture Media; Durapatite, 1306-06-5; Minerals; Tetracycline, 60-54-8Tradenames: BonelikeReferences: Chambers, H., Protein synthesis inhibitors and miscellaneous antibacterial agents (2006) Goodman and Gilman{\textquoteright}s - The Pharmacological Basis of Therapeutics, pp. 1173-1202. , Brunton L. (Ed), McGraw-Hill, Germany;Alkan, A., Erdem, E., Gunhan, O., Karasu, C., Histomorphometric evaluation of the effect of doxycycline on the healing of bone defects in experimental diabetes mellitus: a pilot study (2002) J Oral Maxillofac Surg, 60, p. 898; 
Buchter, A., Kleinheinz, J., Meyer, U., Joos, U., Treatment of severe peri-implant bone loss using autogenous bone and a bioabsorbable polymer that delivered doxycycline (Atridox TM) (2004) Brit J Oral Maxillofacial Surg, 42, pp. 452-456; 
Ramamurthy, N., Rifkin, B., Greenwald, R., Xu, J.-W., Liu, Y., Turner, G., Inhibition of matrix metalloproteinase-mediated periodontal bone loss in rats: a comparison of 6 chemically modified tetracyclines (2005) J Periodontol, 73, pp. 726-734; 
Golub, L., Ramamurthy, N., Kaneko, H., Sasaki, T., Rifkin, B., McNamara, T., Tetracycline administration prevents diabetes-induced osteopenia in the rat: initial observations (1990) Res Commun Chem Pathol Pharmacol, 68, p. 27; 
Williams, S., Wakisaka, A., Zeng, Q., Barnes, J., Martin, G., Wechter, W., Minocycline prevents the decrease in bone mineral density and trabecular bone in ovariectomized aged rats (1996) Bone, 19, pp. 637-644; 
Williams, S., Wakisaka, A., Zeng, Q., Barnes, J., Seyedin, S., Martin, G., Effect of minocycline on osteoporosis (1998) Adv Dental Res, 12, pp. 71-75; 
Pytlik, M., Folwarczna, J., Janiec, W., Effects of doxycycline on mechanical properties of bones in rats with ovariectomy-induced osteopenia (2004) Calcified Tissue Int, 75, pp. 225-230; 
Holmes, S., Still, K., Buttle, D., Bishop, N., Grabowski, P., Chemically modified tetracyclines act through multiple mechanisms directly on osteoclast precursors (2004) Bone, 35, pp. 471-478; 
Polson, A., Bouwsma, O., McNamara, T., Golub, L., Enhancement of alveolar bone formation after tetracycline administration in squirrel monkeys (2005) J Appl Res Clin Dent, pp. 32-42; 
Yamaguchi, A., Udagawa, T., Sawai, T., Transport of divalent cations with tetracycline as mediated by the transposon Tn10-encoded tetracycline resistance protein (1990) J Biol Chem, 265, pp. 4809-4813; 
Li, S., de Wijn, J., Layrolle, P., de Groot, K., Synthesis of macroporous hydroxyapatite scaffolds for bone tissue engineering (2002) J Biomed Mater Res, 61, pp. 109-120; 
Giannoudis, P., Dinopoulos, H., Tsiridis, E., Bone substitutes: an update (2005) Int J Care Injured, 365, p. 220; 
LeGeros, R., Properties of osteoconductive biomaterials: calcium phosphates (2002) Clin Orthop, 395, pp. 81-98; 
Suchanek, W., Yoshimura, M., Processing and properties of hydroxyapatite-based biomaterials for use as hard tissue replacement implants (1998) J Mater Res, 13, pp. 94-117; 
Lopes, M., Santos, J., Monteiro, F., Knowles, J., Glass reinforced hydroxyapatite: a comprehensive study of the effect of glass composition on the crystallography of the composite (1998) J Biomed Mater Res, 39, pp. 244-250; 
Hench, L., Bioactive materials: the potential for tissue regeneration (1998) J Biomed Mater Res, 41, pp. 511-518; 
Kellomaki, M., Niiranen, H., Puumanen, K.A.N., Waris, T., Tormala, P., Bioabsorbable scaffolds for guided bone, regeneration, and generation (2000) Biomaterials, 21, pp. 2495-2505; 
Santos, J., Reis, R., Monteiro, F., Knowles, J., Hastings, G., Liquid phase sintering of hydroxyapatite by phosphate and silicate glass additions structure and properties of the composites (1995) J Mater Sci Mat Med, 6, p. 348; 
Santos, J., Silva, P., Knowles, J., Talal, S., Monteiro, F., Reinforcement of hydroxyapatite by adding P 2O 5-CaO glasses with Na 2O, K 2O and MgO (1996) J Mater Sci Mat Med., 7, p. 187; 
Duarte, F., Santos, J., Afonso, A., Medical applications of Bonelike in maxillofacial surgery (2004) Mater Sci Forum, 370, pp. 455-456; 
Gutierres, M., Hussain, N., Afonso, A., Almeida, L., Cabral, A., Lopes, M., Biological behaviour of bonelike {\textregistered} graft implanted in the tibia of humans (2005) Key Eng Mater, 1041, pp. 284-286; 
Brause, B., Infections with prostheses in bones and joints (2000) Mandell, Douglas and Bennett{\textquoteright}s Principles and Practice of Infectious Diseases. 5th ed., pp. 1196-1200. , Mandell G., Bennet J., and Dolin R. (Eds), Churchill Livingstone, Philadelphia; 
Darley, E., MacGowan, A., Antibiotic treatment of Gram-positive bone and joint infections (2004) J Antimicrobial Chemotherapy, 53, pp. 928-935; 
Zucchelli, G., Sforza, N., Clauser, C., Cesari, C., De Sanctis, M., Topical and systemic antimicrobial therapy in guided tissue regeneration (1999) J Periodontol, 70, pp. 239-247; 
Yukna, R., Sepe, W., Clinical evaluation of localized periodontosis defects treated with freeze-dried bone allografts combined with local and systemic tetracyclines (1982) Int J Periodontics Restorative Dent, 2, pp. 8-21; 
Shirtliff, M., Mader, J., Acute septic arthritis (2002) Clin Microbiol Rev, 15, pp. 527-544; 
Sunder, M., Babu, N., Victor, S., Kumar, K., Kumar, T., Biphasic calcium phosphates for antibiotic release (2005) Trends Biomater Artif Organs, 18, pp. 213-218; 
Lieberman, J., Daluiski, A., Einhorn, T., The role of growth factors in the repair of bone: biology and clinical applications (2002) J Bone Joint Surg, 84, pp. 1032-1044; 
Carson, J., Bostrom, M., Synthetic bone scaffolds and fracture repair (2007) Injury, 38, pp. S33-S70; 
Reg{\'\i}-Vallet, M., Balas, F., Colilla, M., Manzano, M., Drug confinement and delivery in ceramic implants (2007) Drug Metabol Lett, 1, pp. 37-40; 
Lin, T.-L., Lu, F.-M., Conroy, S., Sheu, M.-S., Su, S.-H., Tang, L., Antimicrobial coatings: a remedy for medical device-related infections (2001) Med Device Technol., 12, pp. 26-30; 
Beardmore, A., Brooks, D., Wenke, J., Thomas, D., Effectiveness of local antibiotic delivery with an osteoinductive and osteoconductive bone-graft substitute (2005) J Bone Joint Surg, 87, pp. 107-112; 
Gomes, P., Fernandes, M., Effect of therapeutic levels of doxycycline and minocycline in the proliferation and differentiation of human bone marrow osteoblastic cells (2007) Arch Oral Biol, 52, pp. 251-259; 
Coelho, M., Fernandes, M., Human bone cell cultures in biocompatibility testing Part II. Effect of ascorbic acid, β-glycerophosphate and dexamethasone on osteoblastic differentiation (2000) Biomaterials, 21, pp. 1095-1102; 
J{\o}rgensen, N., Henriksena, Z., S{\o}rensena, O., Civitelli, R., Dexamethasone, BMP-2, and 1,25-dihydroxyvitamin D enhance a more differentiated osteoblast phenotype: validation of an in vitro model for human bone marrow-derived primary osteoblasts (2004) Steroids, 69, pp. 219-226; 
Sakellari, D., Goodson, J., Socransky, S., Kolokotronis, A., Konstantinidis, A., Concentration of 3 tetracyclines in plasma, gingival crevice fluid and saliva (2000) J Clin Periodontol, 27, pp. 53-60; 
Stein, G., Lian, J., Molecular mechanisms mediating proliferation/differentiation interrelationships during progressive development of the osteoblast phenotype (1993) Endocr Rev, 14, pp. 424-442; 
Zhang, Y., Lopes, M., Santos, J., CaO-P 2O 5 Glass ceramics containing bioactive phases: crystallization and in vitro bioactivity studies (2001) Key Eng Mater, 192, pp. 643-646; 
Lopes, M., Knowles, J., Santos, J., Monteiro, F., Olsen, I., Direct and indirect effects of P 2O 5-glass reinforced hydroxyapatite on the growth and function of osteoblast-like cells (2000) Biomaterials, 21, pp. 1165-1172; 
Costa, M., Gutierres, M., Almeida, L., Lopes, M., Santos, J., Fernandes, M., In vitro mineralisation of human bone marrow cells cultured on Bonelike (2004) Key Eng Mater, pp. 254-256. , (821-4); 
Lopes, M., Santos, J., Monteiro, F., Ohtsuki, C., Osaka, A., Kaneko, S., Push-out testing and histological evaluation of glass reinforced hydroxyapatite composites implanted in the tibia of rabbits (2001) J Biomed Mater Res, 54, pp. 463-469; 
Gutierres, M., Hussain, N., Lopes, M., Afonso, A., Cabral, A., Almeida, L., Histological and scanning electron microscopy analyses of bone/implant interface using the novel Bonelike {\textregistered} synthetic bone graft (2006) J Ortho Res, 24, p. 953; 
Kima, H.-W., Leea, E.-J., Kima, H.-E., Salihb, V., Knowles, J., Effect of fluoridation of hydroxyapatite in hydroxyapatite-polycaprolactone composites on osteoblast activity (2005) Biomaterials, 26, pp. 4395-4404; 
Williams, S., Barnes, J., Wakisaka, A., Ogasa, H., Liang, C., Treatment of osteoporosis with MMP inhibitors (1999) Ann NY Acad Sci, 878, pp. 191-200; 
Schwartz, Z., Lohmann, C., Wieland, M., Cochran, D., Dean, D., Textor, M., Osteoblast proliferation and differentiation on dentin slices are modulated by pretreatment of the surface with tetracycline or osteoclasts (2000) J Periodontol, 71, pp. 586-597; 
Bettany, J., Wolowacz, R., Tetracycline derivatives induce apoptosis selectively in cultured monocytes and macrophages but not in mesenchymal cells (1998) Adv Dent Res, 12, pp. 136-143; 
Duewelhenke, N., Krut, O., Eysel, P., Influence on mythochondria and cytotoxicity of different antibiotics administered in high concentrations on primary human osteoblasts and cell lines (2007) Antimicrobial Agents and Chemotherapy, 51, pp. 54-63; 
Golub, L., Wolff, M., Roberts, S., Lee, H.-M., Leung, M., Payonk, G., Treating periodontal diseases by blocking tissue-destructive enzymes (1994) JADA, 125, pp. 163-169; 
Woessner, J., Nagase, H., Inhibition of MMPs (2000) MMPs and Timps, pp. 109-125. , Woessner J., and Nagase H. (Eds), Oxford University Press, Oxford; 
Golub, L., Ramamurthy, N., McNamara, T., Gomes, B., Wolff, M., Casino, A., Tetracyclines inhibit tissue collagenase activity. A new mechanism in the treatment of periodontal disease (1984) J Periodontal Res, 19, pp. 651-655; 
Yu, Z., Ramamurthy, N., Leung, M., Chang, K., McNamara, T., Golub, L., Chemically-modified tetracycline normalizes collagen metabolism in diabetic rats: a dose-response study (1993) J Periodontal Res, 28, pp. 420-428; 
Karimbux, N., Ramamurthy, N., Golub, L., Nishimura, I., The expression of collagen I and XII on RNAs in porphyromonas gingivalis-induced periodontitis in rats: The effect of doxycycline and chemically-modified tetracycline (CMT-1) (1998) J Periodontol, 69, pp. 39-40; 
Ramamurthy, N., Pirila, E., Lee, H.-M., Delayed wound healing, altered collagen level and keratinocyte growth factor expression in skin of ovariectomized rat is normalized by estrogen or chemically modified doxycycline (2003) Menopause - The State of the Art in Research and Management. The Proceedings of the Tenth World Congress, pp. 236-243. , Schneider H. (Ed), Parthenon Publishing Group, New York; 
Gurkan, A., {\c C}unarcuk, S., Huseyinov, A., Adjunctive subantimicrobial dose doxycycline: effect on clinical parameters and gingival crevicular fluid transforming growth factor-b 1 levels in severe, generalized chronic periodontitis (2005) J Clin Periodontol, 32, pp. 244-253; 
Caton, J., Evaluation of Periostat for patient management (1999) Compend Contin Edu Dent, 20, pp. 451-456; 
Salasznyk, R., Williams, W., Boskey, A., Batorsky, A., Plopper, G., Adhesion to vitronectin and collagen I promotes osteogenic differentiation of human mesenchymal stem cells (2004) J Biomed Biotechnol, 1, pp. 24-34; 
Gerstenfeld, L., Chipman, S., Kelly, C., Hodgens, K., Lee, D., Landis, W., Collagen expression, ultrastructural assembly, and mineralization in cultures of chicken embryo osteoblasts (1988) J Cell Biol, 106, pp. 979-989
},
	month = {2008///},
	pages = {630 - 637},
	abstract = {Semi-synthetic tetracyclines are commonly used antibiotics that also seem to play an important role in the modulation of the immuno-inflammatory imbalance, verified in several bone diseases. The association of a therapeutic agent (that prevents bacterial infection and induces tissue formation) to a biomaterial aiming to repair/regenerate bone defects could contribute to a more predictable clinical outcome. The present study intends to evaluate the proliferation and functional activity of osteoblast-induced human bone marrow cells, cultured on the surface of hydroxyapatite (HA) and Bonelike                        {\textregistered}, in the presence of therapeutic concentrations of doxycycline and minocycline. First passage bone marrow cells were cultured for 35 days on the surface of HA and Bonelike
                        {\textregistered} discs, in the absence or presence of 1 μg ml
},
	keywords = {adult, alkaline phosphatase, alkaline phosphatase bone isoenzyme, antimicrobial activity, article, Bacteria (microorganisms), bone infection, bone marrow cell, Bone Marrow Cells, Bone Substitutes, Bonelike, Calcium, calcium phosphate, Cell culture, cell function, cell proliferation, cell survival, cell viability, Cells, Cultured, concentration response, controlled study, Culture Media, culture medium, doxycycline, Durapatite, enzyme activity, extracellular matrix, glass, human, human cell, Humans, hydroxyapatite, implant, Microscopy, Electron, Scanning, Middle Aged, Minerals, minocycline, osteoblast, Osteoblasts, priority journal, Scanning electron microscopy, stroma cell, Tetracycline, tetracycline derivative, Tetracyclines, X-Ray Diffraction},
	isbn = {17427061 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-41549100057\&partnerID=40\&md5=da9f976ed0f5c4ac2999670cc373bdd3},
	author = {Gomes, P.S. and Santos, J.D. and Fernandes, M.H.}
}
@conference {2332,
	title = {Changes in detrended fluctuation indices with aging in healthy and congestive heart failure subjects},
	booktitle = {Computers in CardiologyComput Cardiol},
	series = {Computers in Cardiology 2008, CAR},
	volume = {35},
	year = {2008},
	note = {Conference code: 75561Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusArt. No.: 4748973CODEN: COCADdoi: 10.1109/CIC.2008.4748973Language of Original Document: EnglishCorrespondence Address: Barquero-P{\'e}rez, {\'O}.; B-013, Universidad Rey Juan Carlos, Camino del Molino s/n, Fuenlabrada-28943 Madrid, Spain; email: obarquero@gmail.comReferences: Goldberger, A.L., Nonlinear dynamics, fractals, and chaos theory: Implications for neuroautonomic heart rate control in health and disease (1999) The Autonomic Nervous System, , Bolis C, Licinnio J eds, Geneva: World Health Organization;Peng, C.K., Havlin, S., Stanley, H.E., Goldberger, A.L., Quantification of scaling exponents and crossover phenomena in nonstationary heartbeat time series (1995) Chaos, 5, pp. 82-87; 
Goldberger, A.L., Amaral, L.A.N., Hausdorff, J.M., Ivanov, P.C., Peng, C.K., Stanley, H.E., Fractal dynamics in physiology: Alterations with disease and aging (2002) Proceedings of the National Academy of Sciences, 99, p. 119; 
Berger, R.D., Broken fractals: Where{\textquoteright}s the break? (2001) Journal of Cardiovascular Electrophysiology, 12 (1), pp. 33-35; 
Mandelbrot, B.B., van Ness, J.W., Fractional Brownian motions, fractional noises and applications (1968) SIAM Review, 10, pp. 422-437; 
Kantelhardt, J.W., Zschiegner, S.A., Koscielny-Bunde, E., Havlin, S., Bunde, A., Stanley, H.E., Multifractal detrended fluctuations analysis of nonstationary time series (2002) Physica A, 316, pp. 87-114; 
Goldberger, A.L., Amaral, L.A.N., Glass, L., Hausdorff, J.M., Ivanov, P.C., Mark, R.G., Mietus, J.E., Stanley, H.E., PhysioBank, PhysioToolkit, and PhysioNet: Components of a new research resource for complex physiologic signals (2000) Circulation, 101 (23), pp. e215-e220. , June 13; 
Malik, M., Cripps, T., Farrell, T., Camm, A., Prognostic value of heart rate variability after myocardial infarction. a comparison of different data-processing methods (1989) Medical and Biological Engineering and Computing, 27, pp. 603-621; 
Huikuri, H.V., M{\"a}kikallio, T.H., Perki{\"o}m{\"a}ki, J., Measurement of heart rate variability by methods based on nonlinear dynamics (2003) Journal of Electrocardiology, 36, pp. 95-99; 
Rojo-{\'A}lvarez, J., S{\'a}nchez-S{\'a}nchez, A., Barquero-Prez, O., Goya-Esteban, R., Everss, E., Mora-J{\'\i}menez, I., Garc{\'\i}a-Alberola, A., Analysis of physiological meaning of detrended fluctuation analysis in heart rate variability using a lumped parameter model (2007) Computers in Cardiology, 34, pp. 25-28
},
	month = {2008///},
	pages = {45 - 48},
	address = {Bologna},
	abstract = {Detrended Fluctuation Analysis (DFA) aims to quantify the fractal correlation properties in nonstationary time series, and it has successfully been applied in the assessment of the Heart Rate Variability (HRV) for cardiac risk stratification purposes. However the physiological meaning of DFA indices and its relation with aging have not yet been completely established. Given that a loss of complexity in the physiological regulation of elderly subjects has been reported in the literature, we hypothesized that DFA indices could be modified by aging. In this work we computed the Hurst exponent (estimated using DFA method) and DFA indices α                        1 and α
                        2 to assess the HRV in healthy and Congestive Heart Failure (CHF) subjects, and we studied the dependence of these indices on aging for both healthy and CHF subjects. We found that only α2 and Hurst exponent, and only in the case of healthy subjects, have significant discrimination capability to distinguish between young and elderly groups, and also that these indices have a steady increase with aging. Therefore, we can conclude that the loss of complexity due to aging can be quantified by changes in the values of α
                        2 and Hurst exponent.
},
	keywords = {Cardiology, Congestive heart failures, Correlation properties, Detrended fluctuation analysis, DFA methods, Healthy subjects, Heart rate variabilities, Heart valve prostheses, Hurst exponents, Non-stationary time series, Risk analysis, Risk perception, Risk stratifications, Table lookup, Time series, Time series analysis},
	isbn = {02766574 (ISSN); 1424437067 (ISBN); 9781424437061 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-62249107413\&partnerID=40\&md5=f528b40d49f9968db744c5cd19069493},
	author = {Barquero-P{\'e}rez, {\'O}. and De S{\'a}, J.M. and Rojo-{\'A}lvarez, J.L. and Goya-Esteban, R.}
}
@conference {2326,
	title = {Characterization of heart rate variability loss with aging and heart failure using sample entropy},
	booktitle = {Computers in CardiologyComput Cardiol},
	series = {Computers in Cardiology 2008, CAR},
	volume = {35},
	year = {2008},
	note = {Conference code: 75561Export Date: 21 November 2012Source: ScopusArt. No.: 4748972CODEN: COCADdoi: 10.1109/CIC.2008.4748972Language of Original Document: EnglishCorrespondence Address: Goya-Esteban, R.; Universidad Rey Juan Carlos, Camino del Molino s/n, Fuenlabrada-28943 Madrid, Spain; email: rebegoya@gmail.comReferences: Pincus, S.M., Approximate entropy as a measure of system complexity (1991) Proc Natl Acad Sci, 88, pp. 2297-2301;Pincus, S.M., Goldberger, A.L., Physiological time-series analysis: What does regularity quantify? (1994) Am J Physiol Heart Circ Physiol, 35, pp. 1643-1656; 
Pincus, S.M., Assesing serial irregularity and its implications for health (2001) Annals New York Academy of Sciences, 954, pp. 245-267; 
Marques-de, S.J., Characterization of fetal heart rate using approximate entropy (2005) IEEE Computers in Cardiology, 32, pp. 671-673; 
Richman, J.S., Moorman, J.R., Physiological time-series analysis using approximate entropy and sample entropy (2000) Am J Physiol Heart Circ Physiol, 278, pp. 2039-2049; 
Goldberger, A.L., Amaral, L.A.N., Glass, L., Hausdorff, J.M., Ivanov, P.C., Mark, R.G., Mietus, J.E., Stanley, H.E., PhysioBank, PhysioToolkit, and PhysioNet: Components of a new research resource for complex physiologic signals (2000) Circulation, 101 (23), pp. e215-e220. , June 13; 
Malik, M., Cripps, T., Farrell, T., Camm, A., Prognostic value of heart rate variability after myocardial infarction. a comparison of different data-processing methods (1989) Medical and Biological Engineering and Computing, 27, pp. 603-621; 
Signorini, M., Ferrario, M., Marchetti, M., Marseglia, A., Nonlinear analysis of heart rate variability signal for the characterization of cardiac heart failure patients (2006) Conf Proc IEEE Eng Med Biol Soc, 1 (1), pp. 3431-3434
},
	month = {2008///},
	pages = {41 - 44},
	address = {Bologna},
	abstract = {Entropy based measures, such as Sample Entropy (SampEn), have been widely used for quantifying the Heart Rate Variability (HRV) for cardiac risk stratification purposes, with the hypothesis that decreasing entropy points to a perturbation of the complex physiological mechanisms or disease. However, in the literature, higher entropy values have been reported for some pathologies than for healthy subjects, which could be due to the use of a threshold value r tuned relative to the signal standard deviation. In the present paper we apply SampEn to assess the variability of the RR time series from healthy subjects and subjects with Congestive Heart Failure (CHF) in order to discriminate between both groups, as well as to characterize the variability loss due to aging. We conclude that the use of a fixed threshold value r in the SampEn algorithm instead of its conventional setting (as a percentage of the standard deviation of each data series), improves the discrimination capabilities between healthy and CHF subjects, and it allows to quantify the loss of HRV due to aging in healthy subjects.},
	keywords = {Cardiology, Congestive heart failures, Data series, entropy, Entropy values, Fixed thresholds, Healthy subjects, Heart failures, Heart rate variabilities, Heart valve prostheses, Physiological mechanisms, Risk stratifications, Sample entropies, Signal standard deviations, Standard deviations, Table lookup, Time series},
	isbn = {02766574 (ISSN); 1424437067 (ISBN); 9781424437061 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-62249148798\&partnerID=40\&md5=8e431df62b6a4b2d13e5d71570608d91},
	author = {Goya-Esteban, R. and De S{\'a}, J.P.M. and Rojo-{\'A}lvarez, J.L. and Barquero-P{\'e}rez, O.}
}
@article {2348,
	title = {Chromatographic pattern classification},
	journal = {IEEE Transactions on Biomedical EngineeringIEEE Trans. Biomed. Eng.},
	volume = {55},
	year = {2008},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusArt. No.: 8CODEN: IEBEAdoi: 10.1109/TBME.2008.919852PubMed ID: 18714832Language of Original Document: EnglishCorrespondence Address: Sousa, A. V.; Instituto de Engenharia Biom{\'e}dica (INEB), Instituto Superior de Engenharia Do Porto, 4200-465 Porto, Portugal; email: ats@isep.ipp.ptChemicals/CAS: Hydrolases, EC 3.-References: Keir, G., Winchester, B., Clayton, P., Carbohydrate-deficient glycoprotein syndromes: Inborn errors of protein glycosylation (1999) Ann. Clin. Biochem, 36 (1), pp. 20-36;Durand, G., Seta, N., Protein glycosylation and diseases: Blood and urinary oligosaccharides as markers for diagnosis and therapeutic monitoring (2000) Clin. Chem, 46, pp. 795-805; 
Scriver, S., Beaudet, A., Sly, W., Valle, D., (2001) The Metabolic Bases of Inherited Disease, 3. , New-York: McGraw-Hill; 
Pinto, R., Caseiro, C., Lemos, M., Lopes, L., Fontes, A., Miranda, M., Prevalence of lysosomal storage diseases in Portugal (2004) Eur. J. Hum. Genet, 12, pp. 87-92; 
Friedman, R., Williams, M., Moser, H., Kolodny, E., Improved thin-layer chromatographic method in the diagnosis of mannosidosis (1978) Clin. Chem, 24, pp. 1576-1577; 
Kurczynski, T., Kendzierski, K., Sewell, A., Kuczynski, T., Urinary oligosaccharides in pregnant or lactating women: Pitfall in screening (1993) Clin. Chem, 39, pp. 2346-2347; 
Sewell, A., Urinary oligosaccharides (1991) Techniques Diagnostic Human Biochemical Genetics, pp. 219-231. , New York: Wiley; 
Reich, E., Blatter, A., Modern TLC: A key technique for identification and quality control of botanicals and dietary supplements (2005) J. Planar Chromatogr. - Modern TLC, 18, pp. 34-37. , Jan./Feb; 
Duvdevani-Bar, S., Edelman, S., Visual recognition and categorization on the basis of similarities to multiple class prototypes (1999) Int. J. Comput. Vis, 33, pp. 201-228; 
Pekalska, E., Duin, R., Dissimilarity representations allow for building good classifiers (2002) Pattern Recognit. Lett, 23, pp. 943-956; 
Goldstone, R., Son, J., Similarity (2005) Cambridge Handbook of Thinking and Reasoning, pp. 13-36. , K. Holyoak and R. Morrison, Eds. Cambridge, MA: Cambridge Univ. Press; 
Lavine, B., Clustering and classification of analytical data (2000) Encyclopedia of Analytical Chemistry: Instrumentation and Applications, pp. 9689-9710. , R. Meyers, Ed. Chichester, U.K, Wiley; 
Japkowicz, N., Learning from imbalanced data sets: A comparison of various strategies (2000) Proc. Learn. Imbalanced Data, pp. 10-15; 
Weiss, G., Mining with rarity: A unifying framework (2004) Sigkdd Explor, 6, pp. 7-19; 
Kubat, M., Holte, R., Matwin, S., Machine learning for the detection of oil spills in satellite radar images (1998) Mach. Learn, 30, pp. 195-215; 
Tax, D., One-class classification; concept-learning in the absence of counter-examples, (2001), ASCI, pp. 1-190. , Ph.D. dissertation, Delft Univ. Technol, Dissertation Ser, ppSousa, A., Aguiar, R., Mendon{\c c}a, A., Campilho, A., Automatic lane and band detection in images of thin layer chromatography (2004) Lecture Notes in Computer Science, 3212, pp. 158-165. , Image Analysis and Recognition, New York: Springer-Verlag; 
Schwedt, G., (1997) The Essential Guide to Analytical Chemistry, , New York: Wiley; 
Bajla, I., Hollander, I., Fluch, S., Burg, K., Kollar, M., An alternative method for electrophoretic gel image analysis in the GelMaster software (2005) Comput. Methods Programs Biomed, 77, pp. 209-231; 
Eibrand, R., Kennedy, P., Cotter, D., MacEvilly, U., Wu, B., Analysis of Atlantic salmon skin mucus: COPS - A computer-based system for protein pattern analysis of 1D SDS-PAGE gels (2003) Proc. IEEE Symp. Bioinf. BioEng, pp. 45-52; 
Gerasimov, A., Use of the software processing of scanned chromatogram images in quantitative planar chromatography (2004) J. Anal. Chem, 59, pp. 348-353; 
Goulding, P., (2000) Electrophoresis Gel Analysis Software: Important Aspects, p. 14. , Cambridge, U.K, UVItec Ltd; 
Jedra, M., El Khattabi, N., Limouri, M., Essaid, A., Recognition of seed varieties using a time-delay neural network: Analysis of electrophoretic images (1999) Comput. Electron. Agric, 22, pp. 1-10; 
Machado, A., Campos, M., Siqueira, A., Carvalho, O., An iterative algorithm for segmenting lanes in gel electrophoresis images (1997) Proc. Comput. Graph. Image Process. X Braz. Symp, pp. 140-146. , Campos do Jordao, Brazil, Oct; 
Ye, X., Suen, C., Cheriet, M., Wang, E., A recent development in image analysis of electrophoresis gels (1999) Proc. Vis. Interface, pp. 432-438; 
Shadle, S., Allen, D., Guo, H., Pogozelski, W., Bashkin, J., Tullius, T., Quantitative analysis of electrophoresis data: Novel curve fitting methodology and its application to the determination of a protein-DNA binding constant (1997) Nucleic Acids Res, 25, pp. 850-860; 
Bajla, I., Hollander, I., Burg, K., Improvement of electrophoretic gel image analysis (2001) Meas. Sci. Rev, 1, pp. 5-10; 
Bajla, I., Hollander, I., Burg, K., Fluch, S., A novel approach to quantitative analysis of electrophoretic gel images of DNA fragments (2002) Proc. IEEE Int. Symp. Biomed. Imag, pp. 899-902; 
Lonni, A., Scarminio, I., Silva, L., Ferreira, D., Differentiation of species of Baccharis Genus by HPLC and chemometrics methods (2003) Anal. Sci, 19, pp. 1013-1017; 
Beltr{\'a}n, N., Duarte-Mermoud, M., Salah, S., Bustos, M., Feature selection algorithms using Chilean wine chromatograms as examples (2005) J. Food Eng, 67, pp. 483-490; 
Landgrebe, D., Information extraction principles and methods for multispectral and hyperspectral image data (2000) Information Processing for Remote Sensing, , Hackensack, NJ: World Scientific, ch. 1; 
Landgrebe, D., Hyperspectral image data analysis as a high dimensional signal processing problem (2002) IEEE Signal Process. Mag, 19 (1), pp. 17-28. , Jan; 
Jimenez, L., Landgrebe, D., Hyperspectral data analysis and feature reduction via projection pursuit (1999) IEEE Trans. Geosci. Remote Sens, 37 (6), pp. 2653-2667. , Nov; 
Jimenez, L., Landgrebe, D., Supervised classification in high- dimensional space: Geometrical, statistical, and asymptotical properties of multivariate data (1998) IEEE Trans. Syst., Man, Cybern, 28 (1), pp. 39-54. , Pt. C, Feb; 
Marques deS{\'a}, J., (2001) Pattern Recognition - Concepts, Methods and Applications, , Berlin, Germany: Springer-Verlag; 
Heijden, F., Duin, R., Ridder, D., Tax, D., (2004) Classification, Parameter Estimation and State Estimation, , New York: Wiley; 
Dudoit, S., Fridlyand, J., Speed, T., Comparison of discrimination methods for the classification of tumors using gene expression data (2002) J. Amer. Stat. Assoc, 97, pp. 77-87; 
Zomer, S., Brereton, R., Carter, J., Eckers, C., Support vector machines for the discrimination of analytical chemical data: Application to the determination of tablet production by pyrolysis-gas chromatography-mass spectrometry (2004) Analyst, 129, pp. 175-181; 
Zomer, S., Guillo, C., Brereton, R., Hanna-Brown, M., Toxicological classification of urine samples using pattern recognition techniques and capilarity electrophoresis (2003) Anal. Bioanal. Chem, 378, pp. 2008-2020; 
Fan, X., Bai, J., Shen, P., Diagnosis of breast cancer using HPLC metabonomics fingerprints coupled with computational methods (2005) 27th Annu. Conf. Eng. Med. Biol, pp. 6081-6084. , Shanghai, China; 
Skurichina, M., Raudys, S., Duin, R., K-nearest neighbors directed noise injection in multilayer perceptron training (2000) IEEE Trans. Neural Netw, 11 (2), pp. 504-511. , Mar; 
Ha, T., Bunke, H., Off-line, handwritten numeral recognition by perturbation method (1997) IEEE Trans. Pattern Anal. Mach. Intell, 19, pp. 535-539; 
Lee, S., Noisy replication in skewed binary classification (2000) Comput. Stat. Data Anal, 34, pp. 165-191; 
Demant, C., Streicher-Abel, B., Waszkewitz, P., (1999) Industrial Image Processing, , New York: Springer-Verlag; 
Dempster, A., Laird, N., Rubin, D., Maximum likelihood from incomplete data via the EM algorithm (1977) J. Roy. Stat. Soc. B, 39 (1), pp. 1-38; 
Mor{\'e}, J., Sorensen, D., Computing a trust region step (1983) SIAM J. Sci. Stat. Comput, 4, pp. 553-572; 
Byrd, R., Schnabel, R., Shultz, G., Approximate solution of the trust region problem by minimization over two-dimensional subspaces (1988) Math. Program, 40, pp. 247-263; 
Sousa, A., Mendon{\c c}a, A., Campilho, A., Aguiar, R., Miranda, C., Feature extraction for classification of thin-layer chromatography images (2005) Lecture Notes in Computer Science, 3656, pp. 974-981. , Image Analysis and Recognition, New York: Springer-Verlag; 
Ripley, B., (2004) Pattern Recognition and Neural Networks, , New York: Cambridge Univ. Press; 
Visa, S., Ralescu, A., Learning imbalanced and overlapping classes using fuzzy sets (2003) ICML 2003, Workshop Learn. Imbalanced Datasets, 2. , presented at the, Washington, DC
},
	month = {2008///},
	pages = {1687 - 1696},
	abstract = {In this paper, we propose and evaluate methodologies for the classification of images from thin-layer chromatography. Each individual sample is characterized by an intensity profile that is further represented into a feature space. The first steps of this process aim at obtaining a robust estimate of the intensity profile by filtering noise, reducing the influence of background changes, and by fitting a mixture of Gaussians. The resulting profiles are represented by a set of appropriate features trying to characterize the state of nature, here spread out over four classes, one for normal subjects and the other three corresponding to lysosomal diseases, which are disorders responsible for severe nerve degeneration. For classification purposes, a novel solution based on a hierarchical structure is proposed. The main conclusion of this paper is that an automatically generated decision tree presents better results than more conventional solutions, being able to deal with the natural imbalance of the data that, as consequence of the rarity of lysosomal disorders, has very few representative cases in the disease classes when compared with the normal population. {\textcopyright} 2008 IEEE.},
	keywords = {Acoustic variables control, Algorithms, article, Chromatographic analysis, Chromatographic pattern features, Chromatographic profiles, Chromatography, Chromatography, Thin Layer, Classification, Diagnosis, Computer-Assisted, Gaussian noise (electronic), Hierarchical classifier, Humans, Hydrolases, imaging, Lysosomal Storage Diseases, lysosome storage disease, mathematical analysis, nerve degeneration, noise, normal distribution, Pattern recognition, Pattern Recognition, Automated, Reproducibility of Results, sensitivity and specificity, Urinalysis},
	isbn = {00189294 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-44349183146\&partnerID=40\&md5=df677f44d528c936310d8c547624a550},
	author = {Sousa, A.V. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2364,
	title = {A clinical report of bone regeneration in maxillofacial surgery using bonelike{\textregistered} synthetic bone graft},
	journal = {Journal of Biomaterials ApplicationsJ. Biomater. Appl.},
	volume = {22},
	year = {2008},
	note = {Cited By (since 1996): 6Export Date: 21 November 2012Source: ScopusCODEN: JBAPEdoi: 10.1177/0885328207078260Language of Original Document: EnglishCorrespondence Address: Santos, J.D.; Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua Campo Alegre 823, 4150-180 Porto, Portugal; email: jdsantos@fe.up.ptChemicals/CAS: Bone SubstitutesReferences: Mohamed, A., Sudha, K., Kim, F., Karl, K., Scott, P.B., Bone Graft Substitutes (2003) Cell Based Approaches for Bone Graft Substitutes, p. 127. , In: Laurencin, C.T. (ed.), Chapter 7 ASTM - International, USA;Mellonig, J.T., Prewett, A.B., Moyer, M.P., HIV Inactivation in a Bone Allograft (1992) J. Periodontol., 63, p. 979; 
Buck, B.E., Malinin, T.I., Brown, M.D., Bone Transplantation and Human Immunodeficiency Virus. An Estimate of Risk of Acquired Immunodeficiency Syndrome (AIDS) (1989) Clin. Orthop., 240, p. 129; 
Mary, E.A.R., Raymond, A.Y., Bone Replacement Grafts - The Bone Substitutes (1998) Dent. Clin. North Am., 42 (3), p. 491; 
Doron, I.I., Amy, L.L., Bone Graft Substitutes (2002) Oper. Tech. Plast. Reconsr. Surg., 9 (4), p. 151; 
Wright, S., Commentary The Bone-Graft Market in Europe, in Emerging Technologies in Orthopedics I: Bone Graft Substitutes (1999) Bone Growth Stimulators and Bone Growth Factors By Datamonitor Plc, p. 591; 
Knaack, D., Goad, M.E.P., Aiolova, M., Christian, R., Ali, T., Promod, C., Duke Lee, D., Resorbable Calcium Phosphate Bone Substitute (1998) J. Biomed. Mater. Res., 43, p. 399; 
Frayssinet, P., Trouillet, J.L., Rouquet, N., Azimus, E., Autefage, A., Osseointegration Macroporous Calcium Phosphate Ceramics Having a Different Chemical Composition (1993) Biomaterials, 14, p. 423; 
Klein, C.P.A.T., Driessen, A.A., Groot de, K., van den Hooff, A., Biodegradation Behavior of Various Calcium Phosphate Materials in Bone Tissue (1983) J. Biomed. Mater. Res., 17, p. 769; 
Daculsi, G., Biphasic Calcium Phosphate Concept Applied to Artificial Bone, Implant Coating and Injectable Bone Substitute (1998) Biomaterials, 19, p. 1473; 
Santos, J.D., Hastings, G.W., Knowles, J.C., Sintered Hydroxyapatite Compositions and Method for the Preparation Thereof (1999) Worldwide Applications, , (PCT), Patent No. 1 189 851; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Knowles, J.C., Glass Reinforced Hydroxyapatite: A Comprehensive Study of the Effect of Glass Composition on the Crystallography of the Composite (1998) J. Biomed. Mater. Res., 39, p. 244; 
Lopes, M.A., Monteiro, F.J., Santos, J.D., Glass-reinforced Hydroxyapatite Composites: Fracture Toughness and Hardness Dependence on Microstructural Characteristics (1999) Biomaterials, 20, p. 2085; 
Lopes, M.A., Silva, R.F., Monteiro, F.J., Santos, J.D., Microstructural Dependence of Young{\textquoteright}s and Shear Moduli of P 2 O 2 Glass Reinforced Hydroxyapatite for Biomedical Applications (2000) Biomaterials, 21, p. 749; 
Lopes, M.A., Santos, J.D., Monteiro, F.J., Ohtsuki, C., Osaka, A., Kaneko, S., Inoue, H., Push-out Testing and Histological Evaluation of Glass Reinforced Hydroxyapatite Composites Implanted in the Tibia of Rabbits (2001) J. Biomed. Mater. Res., 54, p. 463; 
Lobato, J.V., Hussain, N.S., Botelho, C.M., Rodrigues, J.M., Lu{\'\i}s, A.L., Maur{\'\i}cio, A.C., Lopes, M.A., Santos, J.D., Assessment of the Potential of Bonelike{\textregistered} Graft for Bone Regeneration by using an Animal Model (2005) Key. Eng. Mater., 877, pp. 284-286; 
Lobato, J.V., Hussain, N.S., Botelho, C.M., Mauricio, A.C., Afonso, A., Ali, N., Santos, J.D., Assessment of Bonelike{\textregistered} Graft with a Resorbable Matrix using an Animal Model (2006) Thin Solid Films, 515, p. 362; 
Duarte, F., Santos, J.D., Afonso, A., Medical Applications of Bonelike{\textregistered} in Maxillofacial Surgery (2004) Mater. Sci. Forum, 370, pp. 455-456; 
Gutierres, M., Hussain, N.S., Afonso, A., Almeida, L., Cabral, T., Lopes, M.A., Santos, J.D., Biological Behaviour of Bonelike{\textregistered} Graft Implanted in Tibia of Humans (2005) Key Eng. Mater., 1041, pp. 284-286; 
LeGeros, R.Z., Properties of Osteoconductive Biomaterials: Calcium Phosphates (2002) Clin. Orthop., 395, p. 81; 
Robert, D.A.G., Hanneke, G.T., Ronald, J.H., Buma, P., Mechanism of Bone Incorporation of β-TCP Bone Substitute in Open Wedge Tibial Osteotomy in Patients (2005) Biomaterials, 26, p. 6713; 
Hirotsugu, H., Norio, A., Kimimitsu, O., Noritaka, F., Ohnishi, H., Okada, A., Nomura, S., Maeda, T., A Histological Evaluation on Self setting α-tricalcium Phosphate Applied in the Rat Bone Cavity (2004) Biomaterials, 25, p. 431; 
Meinel, L., Betz, O., Fajardo, R., Hofmann, S., Nazarian, A., Cory, E., Hilbe, M., Kirker-Head, C., Silk Based Biomaterials to Heal Critical Sized Femur Defects (2006) Bone, 39, p. 922; 
Lim, S.C., Lee, M.J., Yeo, H.H., Effects of Various Implant Materials on Regeneration of Calvarial Defects in Rats (2000) Pathol. Int., 50, p. 594; 
Carano, R.A.D., Filvaroff, E.H., Angiogenesis and Bone Repair (2003) Drug Discov. Today, 21, p. 980; 
Gutierres, M., Hussain, N.S., Lopes, M.A., Afonso, A., Cabral, A.T., Almeida, L., Santos, J.D., Histomorphometric Measurements Histological and SEM Analyses of bone/ implant Interface: Clinical Trials using Bonelike{\textregistered} Granules (2006) J. Orthop. Res., 24, p. 953
},
	month = {2008///},
	pages = {373 - 385},
	abstract = {The objective of this study is to evaluate the osteoconductivity and bioactivity of the Bonelike{\textregistered} graft in repairing surgical cystic bone defects. Bonelike{\textregistered} is implanted in 11 patients, aged between 24 and 53 years with a mean age of 36 years, consisting of 5 men and 6 women. According to the standard follow up protocols, radiological examinations are performed and Bonelike{\textregistered} /bone retrieved samples have been analyzed histologically using non-decalcified sections obtained perpendicular to bone length axis. Radiographic examination and histological results clearly demonstrate an extensive new bone formation apposed on Bonelike{\textregistered} granules with a significant degree of maturation. These clinical applications in maxillary bone defects indicate perfect bone bonding between new bone formed and Bonelike{\textregistered} granules, along with partial surface biodegradation. This quick and effective osteoconductive response from Bonelike{\textregistered} may reduce the time needed to reconstruct the bone defected area of patients. {\textcopyright} 2008 Sage Publications.},
	keywords = {adult, article, Bioactivity, Bone, bone prosthesis, Bone Regeneration, Bone Substitutes, bone transplantation, Bonelike{\textregistered}, clinical trial, facial bone, Facial Bones, Female, Histological studies, histology, human, Humans, Implants (surgical), instrumentation, male, Maxillofacial surgery, methodology, Middle Aged, oral surgery, Pathology, Radiography, Radiology, Regeneration, Surgery, Surgery, Oral, Synthetic bone graft, treatment outcome},
	isbn = {08853282 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-37249042186\&partnerID=40\&md5=ab2c9e9614d3f4f521445c149cff57ec},
	author = {Sousa, R.C. and Lobato, J.V. and Maur{\'\i}cio, A.C. and Hussain, N.S. and Botelho, C.M. and Lopes, M.A. and Santos, J.D.}
}
@article {2342,
	title = {Comparative study of nanohydroxyapatite microspheres for medical applications},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {86},
	year = {2008},
	note = {Cited By (since 1996): 22Export Date: 21 November 2012Source: ScopusCODEN: JBMRCdoi: 10.1002/jbm.a.31634PubMed ID: 17975824Language of Original Document: EnglishCorrespondence Address: Mateus, A. Y. P.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: yovana@ibmc.up.ptChemicals/CAS: alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; calcium hydroxide, 1305-62-0; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; calcium, 7440-70-2; dodecyl sulfate sodium, 151-21-3; neutral red, 553-24-2; phosphoric acid, 7664-38-2; Biocompatible Materials; Durapatite, 1306-06-5; PowdersReferences: Jarcho, M., Calcium phosphates ceramics as hard tissue prosthetics (1981) Clin Orthop Relat Res, 157, pp. 259-278;Bucholz, R., Carlton, A., Holmes, R., Hydroxyapatite and tricalcium phosphate bone graft substitutes (1987) Orthop Clin N Am, 18, pp. 323-334; 
Daculsi, G., Passuti, N., Martins, S., Deudon, C., LeGeros, R., Raher, S., Macroporous calcium phosphate ceramic for ling bone surgery in humans and dogs: Clinical and histological study (1990) J Biomed Mater Res, 24, pp. 379-396; 
Gauthier, O., Bouler, J., Weiss, P., Bosco, J., Daculsi, G., Aguado, E., Kinetic study of bone in growth and ceramic resorption associated with the implantation of different injectable calcium-phosphate bone substitutes (1999) J Biomed Mater Res, 47, pp. 28-35; 
Rey, C., Calcium phosphate biomaterials and bone mineral: Differences in composition, structures and properties (1990) Biomaterials, 11, pp. 13-15; 
Yuan, H., Kurashina, K., de Bruijn, J., Li, Y., de Groot, K., Zhang, X., A preliminary study on osteoinduction of two kinds of calcium phosphate ceramics (1999) Biomaterials, 20, pp. 1799-1806; 
Liou, S., Chen, S., Liu, D., Synthesis and characterization of needlelike apatitic nanocomposite with controlled aspect ratios (2003) Biomaterials, 24, pp. 3981-3988; 
Earl, J.S., Wood, D.J., Mile, S.J., Hydrothermal synthesis of hydroxyapatite (2006) J Phys Conf, 26, pp. 268-271; 
Han, J.K., Song, H.Y., Fumio, S., Lee, B.T., Synthesis of high purity nano-sized hydroxyapatite powder by microwave-hydrothermal method (2006) Mater Chem Phys, 99, pp. 235-239; 
Yoshimura, M., Sujaridworakun, P., Koh, F., Fujiwara, T., Pongkao, D., Ahniyaz, A., Hydrothermal conversion of calcite crystals to hydroxyapatite (2004) Mater Sci Eng C, 24, pp. 521-525; 
Bogdanoviciene, I., Beganskiene, A., Tonsuaadu, K., Glaser, J., Meyer, H.J., Kareiva, A., Calcium hydroxyapatite Ca 10(PO 4) 6 (OH) 2 ceramics prepared by aqueous sol-gel processing (2006) Mater Res Bull, 41, pp. 1754-1762; 
Liu, D.M., Troczynski, T., Tseng, W.J., Water-based sol-gel synthesis of hydroxyapatite: Process development (2001) Biomaterials, 22, pp. 1721-1730; 
Bezzi, G., Celotti, G., Landi, E., La Torretta, T.M.G., Sopyan, I., Tampieri, A., A novel sol-gel technique for hydroxyapatite preparation (2003) Mater Chem Phys, 78, pp. 816-824; 
An, G.H., Wang, H.J., Kim, B.H., Jeong, Y.G., Choa, Y.H., Fabrication and characterization of a hydroxyapatite nanopowder by ultrasonic spray pyrolysis with salt-assisted decomposition (2007) Mater Sci Eng A, pp. 449-451,821-824; 
Varma, H.K., Babu, S., Synthesis of calcium phosphate bioceramics by citrate gel pyrolisis method (2005) Ceram Int, 31, pp. 109-114; 
Jarudilokkula, S., Tanthapanichakoonb, W., Boonamnuayvittayaa, V., Synthesis of hydroxyapatite nanoparticles using an emulsion liquid membrane system Colloids Surf A, , Forthcoming; 
Bose, S., Saha, S.K., Synthesis and characterization of hydroxyapatite nanopowders by emulsion technique (2003) Chem Mater, 15, pp. 4464-4469; 
Nayar, S., Sihna, M.K., Basu, D., Sinha, A., Synthesis and sintering of biomimetic hydroxyapatite nanoparticles for biomedical applications (2006) J Mater Sci: Mater Med, 17, pp. 1063-1068; 
Tas, A.C., Synthesis of biomimetic Ca-hydroxyapatite powders at 37{\textdegree}C in synthetic body fluids (2000) Biomaterials, 21, pp. 1429-1438; 
Wanga, A., Yina, H., Liua, D., Wub, H., Wadac, Y., Rena, M., Xua, Y., Chenga, X., Effects of organic modifiers on the size-controlled synthesis of hydroxyapatite nanorods (2007) Appl Surf Sci, 253, pp. 3311-3316; 
Zhang, H.G., Zhu, Q., Surfactant-assisted preparation of fluoride-substituted hydroxyapatite nanorods (2005) Mater Lett, 59, pp. 3054-3058; 
Liu, Y., Houa, D., Wangb, G., A simple wet chemical synthesis and characterization of hydroxyapatite nanorods (2004) Mater Chem Phys, 86, pp. 69-73; 
Pang, Y.X., Bao, X., Influence of temperature, ripening time and calcination on the morphology and crystallinity of hydroxyapatite nanoparticles (2003) J Eur Ceram Soc, 23, pp. 1697-1704; 
Gitzhofer, B.E., Boulos, M.I., Morpholgical study of hydroxyapatite nanocrystal suspension (2000) J Mater Sci: Mater Med, 11, pp. 523-531; 
Barbosa, M., Granja, P., Barrias, C., Amaral, I., Polysaccharides as scaffolds for bone regeneration (2005) ITBM-RBM, 26, pp. 212-217; 
Hari, P., Chandy, T., Sharma, C., Chitosan/calcium-alginate beads for oral delivery of insulin (1996) J Appl Polym Sci, 59, pp. 1796-1801; 
Barrias, C.C., Ribeiro, C.C., Barbosa, M.A., Adhesion and proliferation of human osteoblastic cells seeded on injectable hydroxyapatite microspheres (2004) Key Eng Mater, pp. 254-262,877-880; 
Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., Preparation and characterisation of calcium-phosphate porous microspheres with a uniform size for biomedical applications (2006) J Mater Sci: Mater Med, 17, pp. 455-463; 
Bowersock, T., Martins, S., Vaccine delivery to animals (1999) Adv Drug Deliv Rev, 32, pp. 167-194; 
Kong, H., Smith, M., Mooney, D., Designing alginate hydrogels to maintain viability of immobilized cells (2003) Biomaterials, 24, pp. 4023-4029; 
Legallais, C., David, B., Dor{\'e}, E., Bioartificial livers (BAL): Current technological aspects and future developments (2001) J Membr Sci, 181, pp. 81-95; 
Liu, X.D., Yu, W.Y., Zhang, Y., Xue, W.M., Yu, W.T., Xiong, Y., Ma, X.J., Yuan, Q., Characterization of structure and diffusion behaviour of Ca-alginate beads prepared with external or internal calcium sources (2002) J Microencapsul, 19 (6 -1), pp. 775-782; 
Taqieddin, E., Amiji, M., Enzyme immobilization in novel alginate-chitosan coreshell microcapsules (2004) Biomaterials, 25, pp. 1937-1945; 
Ribeiro, C., Barrias, C., Barbosa, M., Calcium phosphate-alginate microspheres as enzyme delivery matrices (2004) Biomaterials, 25, pp. 4363-4373; 
Kim, Y.J., Park, H.G., Yang, Y.L., Yoon, Y., Kim, S., Oh, E., Multifunctional drug delivery system using starch-alginate beads for controlled release (2005) Biol Pharm Bull, 28, pp. 394-397; 
Barrias, C., Ribeiro, C., Lamghari, M., Sa, C., Barbosa, M., Proliferation, activity and osteogenic differentiation of bone marrow stromal cells cultured on calcium titanium phosphate microspheres (2005) J Biomed Mater Res A, 72, pp. 57-66; 
Mateus, A.Y.P., Ferraz, M.P., Monteiro, F.J., Microspheres based on hydroxyapatite nanoparticles aggregates for bone regeneration (2007) Key Eng Mater, pp. 330-332,243-246; 
Mankani, M., Fowler, B., Kingman, A., Robey, P., In vivo bone formation by human bone marrow stromal cells: Effect of carrier particle size and shape (2001) Biotechonol Bioeng, 72, pp. 96-107; 
Higashi, T., Okamoto, H., Influence of particle size of calcium phosphate ceramics as a capping agent on the formation of a hard tissue barrier in amputated dental pulp (1996) J Endodoncy, 22, pp. 281-283; 
Guo, G., Xun, Y., Wnag, Z., Guo, H., Preparation of hydroxyapatite nanoparticles by reverse microemulsion (2005) Ceram Int, 331, pp. 869-872; 
Sun, X.M., Chen, X., Deng, Z.X., Li, Y.D., A CTAB-assited hydrothermal orientation growth of ZnO nanorods (2002) Mater Chem Phys, 78, pp. 99-104; 
Uota, M., Arakawa, H., Kitamura, N., Yoshimura, T., Tanaka, J., Kijima, T., Synthesis of high surface area hydroxyapatite nanoparticles by mixed surfactant-mediated approach (2005) Langmuir, 21, pp. 4724-4728; 
Balasundaram, G., Sato, M., Webster, T.J., Using hydroxyapatite nanoparticles and decreased crystallinity to promote osteoblast adhesion similar to functionalizing with RGD (2006) Biomaterials, 27, pp. 2798-2805; 
Wutticharoenmongkol, P., Sanchavanakit, N., Pavasant, P., Supaphol, P., Novel bone scaffolds or electrospun polycaprolactone fibers filled with nanoparticles (2006) J Nanosci Nanotechnol, 6, pp. 514-522; 
Gonzalez-McQuire, R., Green, D., Walsh, D., Hall, S., Chane-Ching, J., Oreffo, R.O.C., Mann, S., Fabrication of hydroxyapatite sponges by dextran sulphate/amino acid templating (2005) Biomaterials, 26, pp. 6652-6656; 
Sun, W.B., Wu, Y.F., Ding, Y., Chu, C.L., Liu, T.J., The expression and activity of alkaline phosphatase in human periodontal ligament cells with nanometer hydroxyapatite (2006) Zhonghua Kou Qiang Yi Xue Za Zhi, 41, pp. 348-349; 
Bowers, K., Keller, J.C., Randolph, B., Wick, D., Michaels, C., Optimization of surface micromorphology for enhanced osteoblast responses in vitro (1992) Int J Oral Maxillofac Impl, 7, pp. 302-310; 
Martin, J.Y., Schwartz, Z., Hummert, T.W., Schraub, D.M., Simpson, J., Lankford Jr, J., Dean, D.D., Boyan, B.D., Effect of titanium surface roughness on proliferation, differentiation, and protein synthesis of human osteoblast-like cells (MG63) (1995) J Biomed Mater Res, 29, pp. 389-401; 
Boyan, B.D., Batzer, R., Kieswetter, K., Liu, Y., Cochran, D.L., Szmuckler-Monclers, S., Dean, D.D., Schwartz, Z., Titanium surface roughness alters responsiveness of MG63 osteoblastic-like cells to 1α,25-(OH) 2D 3 (1998) J Biomed Mater Res, 39, pp. 77-85
},
	month = {2008///},
	pages = {483 - 493},
	abstract = {This study concerns the preparation, physical, and in vitro characterization of two different types of hydroxyapatite (HA) microspheres, which are intended to be used as drug-delivery systems and bone-regeneration matrices. Hydroxyapatite nanoparticles (HA-1 and HA-2) were prepared using the chemical precipitation synthesis with H                        3PO
                        4, Ca(OH) 
                        2, and a surfactant, SDS (sodium dodecyl sulfate), as starting reagents. The HA powders were dispersed in a sodium alginate solution, and spherical particles were obtained by droplet extrusion coupled with ionotropic gelation in the presence of Ca
                        2+. These were subsequently sintered to produce HA-1 and HA-2 microspheres with a uniform size and interconnected microporosity. Both powders and microspheres were characterized using FTIR and X-ray diffraction. Moreover, SEM and mercury intrusion porosimetry were used to analyze the microspheres, and TEM was used to analyze the powders. Results showed that pure HA and mixtures of HA/β-TCP in the nanometer range and needlelike shape were obtained for HA-1 and HA-2 powders, respectively. Neutral Red, scanning electron microscopy and confocal microscopy were used to evaluate the behavior of osteoblastic-like MG-63 cells cultured on HA microspheres surfaces for 7 days. Results showed that good adhesion and proliferation of osteoblasts on the HA microspheres surface. Cells built bridges between adjacent microspheres, forming microspheres-cells clusters in both types of materials. {\textcopyright} 2007 Wiley Periodicals, Inc.
},
	keywords = {alginic acid, analytic method, Apatite, article, Biocompatible Materials, Biological materials, biomaterial, Bone Regeneration, Calcium, calcium hydroxide, calcium phosphate, cell adhesion, Cell Line, cell proliferation, Chemical precipitation, Chemotherapy, Comparative studies, comparative study, controlled study, dodecyl sulfate sodium, Drug delivery, Drug delivery system (DDS), Drug Delivery Systems, Drug dosage, Durapatite, Gelation, human, human cell, Humans, hydroxyapatite, Hydroxyapatite (HAP), Hydroxylation, In vitro characterization, in vitro study, infrared spectroscopy, materials testing, Medical applications, Mercury intrusion porosimetry, microsphere, Microspheres, Nano-hydroxyapatite (n-HA), Nanohydroxyapatite, nanoparticle, nanoparticles, neutral red, osteoblast, Osteoblasts, Particle Size, phosphoric acid, porosity, powder, Powders, precipitation, Precipitation (chemical), reagent, Regeneration (renovating), Scanning electron microscopy, surfactant, synthesis, Synthesis (chemical), X ray diffraction},
	isbn = {15493296 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-47049103112\&partnerID=40\&md5=138b3e2f3a97d8b398546d3f85567a0f},
	author = {Mateus, A.Y.P. and Barrias, C.C. and Ribeiro, C. and Ferraz, M.P. and Monteiro, F.J.}
}
@book {2385,
	title = {Characterization of hydroxyapatite sputtered films doped with titanium},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {330-332 I},
	year = {2007},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Ribeiro, A.R.; INEB-Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Universidade do Porto, Rua Campo Alegre, 823, 4150-180 Porto, Portugal; email: arr@ineb.up.ptReferences: Bhat, (2002) Biomaterials, , Narosa Publishing House/Kluwer Academic Publishers, India;Feddes B, Vredenberg AM. Wolke JGC, Jansen JA.: Surface and Coatings Technology 185 (2-3) (2004), p. 346Gisep, A., (2002) Injury-International Journal of the Care of the Injured, 33 (SUPPL. 2), p. 88; 
Ooms, E.M., Wolke, J.G.C., van der Waerden, J.P.C.M., (2002) Journal of Biomedical Materials Research, 61 (1), p. 9; 
Barrias, C.C., Ribeiro, C.C., Rodriguez, D., S{\'a} Miranda, C., Barbosa, M.A., (2005) Key Engineering Materials, 284-286, p. 689; 
Ribeiro, C.C., Barrias, C.C., Barbosa, M.A., (2004) Biomaterials, 25, p. 4363; 
van Dijk, K., Schaeken, H.G., Mar{\'e}e, C.H.M., Verhoeven, J., Wolke, J.C.G., Habraken, F.H.P.M., Jansen, J.A., (1995) Surface and Coatings Technology, 76-77, p. 206; 
Hulshoff, J.E.G., van Dijk, K., de Ruijter, J.E., (1998) Journal of Biomedical Materials Research, 40 (3), p. 464; 
Yang, Y.Z., Kim, K.H., Ong, J.L., (2005) Biomaterials, 26 (3), p. 327; 
Ong, J.L., Lucas, L.C., (1998) Biomaterials, 19 (4-5), p. 455; 
Thian, E.S., Huang, J., Best, S.M., Barber, Z.H., Bonfield, W., (2005) Biomaterials, 26, p. 2947
},
	month = {2007///},
	pages = {649 - 652},
	abstract = {In the present work, thin films of hydroxyapatite (HAp), titanium, and hydroxyapatite doped with different titanium concentrations were obtained by sputtering and characterized using several analytical techniques. These films are intended to be used as model surfaces on protein adsorption studies in order to better understand the role of titanium ions in biological processes.},
	keywords = {Adsorption, Biofilms, Doping (additives), hydroxyapatite, Hydroxyapatite sputtered films, Model surfaces, Proteins, Sputtering, Titanium, Titanium ions},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33846300257\&partnerID=40\&md5=7946215d8f37e0e216b57336b89f0f02},
	author = {Ribeiro, A.R. and Piedade, A.P. and Ribeiro, C.C. and Vieira, M.T. and Barbosa, M.A.}
}
@book {2414,
	title = {Calcium phosphate microspheres for localised delivery of a therapeutic enzyme},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {309-311 II},
	year = {2006},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Barrias, C.C.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, R. Campo Alegre 823, 4150-180 Porto, Portugal; email: ccbarrias@ineb.up.ptReferences: Barrias, C.C., Ribeiro, C.C., Barbosa, M.A., (2004) Key Eng. Mater., 254-256, p. 877;Barrias, C.C., Ribeiro, C.C., Lamghari, M., Miranda, C.S., Barbosa, M.A., (2005) J. Biomed. Mater. Res. A, 72 A, p. 57; 
Scriver, S.R., (2001) The Metabolic Bases of Inherited Disease, , McGraw-Hill, New York; 
Sa Miranda, M.C., Aerts, J.M., Pinto, R., Fontes, A., (1990) Clin. Genet., 38, p. 218; 
Barrias, C.C., Ribeiro, C.C., Barbosa, M.A., (2005) Key Eng. Mater, 284-285, p. 689; 
Qi, X.Y., Grabowski, G.A., (1998) Biochem., 38, p. 11544; 
Barrias, C.C., Martins, M.A., Miranda, M.A., Barbosa, M.A., (2005) Biomaterials, 26, p. 2695
},
	month = {2006///},
	pages = {903 - 906},
	abstract = {The ability of calcium titanium phosphate (CTP) and hydroxyapatite (HAp) microspheres to reversibly adsorb the enzyme glucocerebrosidase (GCR) while preserving its biological activity, and efficiently deliver it to Gaucher disease (GD) fibroblasts was investigated. CTP microspheres adsorbed ca. 3.6-fold more GCR than HAp microspheres. The activity of adsorbed GCR was higher than the free enzyme in the case of CTP microspheres and lower when HAp was used. GCR release from both types of microspheres was characterized by the initial elution of a large percentage of enzyme followed by a delayed release that extended for at least 30 days. Released GCR was internalized by GD fibroblasts increasing their intracellular enzymatic activity. In cells treated with the same amount of GCR-loaded CTP microspheres or free-GCR a higher intracellular activity was detected in the former case, suggesting an improved efficacy.},
	keywords = {Adsorption, Biocompatibility, biological activity, Biomaterials, Calcium Compounds, Calcium titanium phosphate, Calcium titanium phosphate (CTP), Cells, Enzyme adsorption, Enzymes, hydroxyapatite, Microspheres},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33645396489\&partnerID=40\&md5=de3cf8530e8c690b254fed782b964340},
	author = {Barrias, C.C. and Ribeiro, C.C. and Martins, M.C.L. and Barbosa, M.A. and Rodrigues, D. and Miranda, M.C.S.}
}
@article {2409,
	title = {Cellulose phosphates as biomaterials. In vitro biocompatibility studies},
	journal = {Reactive and Functional PolymersReact Funct Polym},
	volume = {66},
	year = {2006},
	note = {Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusCODEN: RFPOFdoi: 10.1016/j.reactfunctpolym.2005.10.027Language of Original Document: EnglishCorrespondence Address: Granja, P.L.; INEB - Instituto de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre 823, 4150-180 Porto, Portugal; email: pgranja@ineb.up.ptReferences: Albrektsson, T., (1998) Handbook of Biomaterial Properties, p. 500. , Black J., and Hastings G. (Eds), Chapman \& Hall, London;Friedman, R.J., Black, J., Galante, J.O., Jacobs, J.J., Skinner, H.B., (1993) J. Bone Joint. Surg., 75 A, p. 1086; 
Puleo, D.A., Nanci, A., (1999) Biomaterials, 20, p. 2311; 
Lamba, N.M.K., Baumgartner, J.A., Cooper, S.L., (1998) Frontiers in Tissue Engineering, p. 121. , Patrick C.W., Mikos A.G., and McIntire L.V. (Eds), Elsevier, Amsterdam; 
Horbett, T.A., (2004) Biomaterials Science: An Introduction to Materials in Medicine. second ed., p. 237. , Ratner B.D., Hoffman A.S., Schoen F.J., and Lemons J.E. (Eds), Elsevier-Academic Press, San Diego; 
McFarland, C.D., Mayer, S., Scotchford, C., Dalton, B.A., Steele, J.G., Downes, S., (1999) J. Biomed. Mater. Res., 44, p. 1; 
Lee, J.H., Lee, H.B., (1993) J. Biomater. Sci. Polym. Ed., 4, p. 467; 
Healy, K.E., Thomas, C.H., Rezania, A., Kim, J.E., McKeown, P.J., Lom, B., Hockberger, P.E., (1996) Biomaterials, 17, p. 195; 
Vogler, E.A., (1993) Surfactant Science Series, 49, p. 183. , Berg J.C. (Ed), Marcel Dekker, New York (Wettability); 
Choee, J.H., Lee, S.J., Lee, Y.M., Rhee, J.M., Lee, H.B., Khang, G., (2004) J. Appl. Polym. Sci., 92, p. 599; 
Faucheux, N., Schweiss, R., Lutzow, K., Werner, C., Groth, T., (2004) Biomaterials, 25, p. 2721; 
Webb, K., Hlady, V., Tresco, P.A., (1998) J. Biomed. Mater. Res., 41, p. 422; 
De Rosa, M., Carteni, M., Petillo, O., Calarco, A., Margarucci, S., Rosso, F., De Rosa, A., Peluso, G., (2004) J. Cell. Physiol., 198, p. 133; 
Habbaba, M.M., Ulgen, K.O., (1997) J. Chem. Technol. Biotechnol., 69, p. 405; 
Lee, J.H., Lee, J.W., Khang, G., Lee, H.B., (1997) Biomaterials, 18, p. 351; 
Keselowsky, B.G., Collard, D.M., Garcia, A.J., (2004) Biomaterials, 25, p. 5947; 
Webb, K., Hlady, V., Tresco, P.A., (2000) J. Biomed. Mater. Res., 49, p. 362; 
Smetana Jr., K., (1993) Biomaterials, 14, p. 1046; 
Jayaraman, M., Meyer, U., Buhner, M., Joos, U., Wiesmann, H.P., (2004) Biomaterials, 25, p. 625; 
Anselme, K., Bigerelle, M., Noel, B., Dufresne, E., Judas, D., Iost, A., Hardouin, P., (2000) J. Biomed. Mater. Res., 49, p. 155; 
von Recum, A.F., Brown, C.E., Shannon, C.E., LaBerge, M., (1999) Handbook of Biomaterials Evaluation: Scientific, Technical and Clinical Testing of Implant Materials. second ed., p. 227. , von Recum A.F. (Ed), Edwards Brothers, Ann Arbor; 
Hon, D.N.-S., (1996) Polysaccharides in Medicinal Applications, p. 87. , Dumitriu D. (Ed), Marcel Dekker, New York; 
Franz, G., (1986) Adv. Polym. Sci., 76, p. 1; 
Ikada, Y., (1989) Cellulose: Structural and Functional Aspects, p. 447. , Kennedy J.F., Phillips G.O., and Williams P.A. (Eds), Ellis Horwood, Chichester; 
Miyamoto, T., Takahashi, S., Ito, H., Inagaki, H., Noishiki, Y., (1989) J. Biomed. Mater. Res., 23, p. 125; 
Dias, G.J., Peplow, P.V., Teixeira, F., (2003) J. Mater. Sci.: Mater. Med., 14, p. 739; 
Uysal, A., Ozbek, S., Ozcan, M., (2003) Plast. Reconstr. Surg., 112, p. 540; 
Galgut, P.N., (1990) Biomaterials, 11, p. 561; 
Skoog, T., (1967) Scand. J. Plast. Reconstr. Surg., 1, p. 113; 
Chauveaux, D., Barbie, C., Barthe, X., Baquey, C., Poustis, J., (1990) Clin. Mater., 5, p. 251; 
Poustis, J., Baquey, C., Chauveaux, D., (1994) Clin. Mater., 16, p. 119; 
Li, S., Liu, Q., de Wijn, J., Wolke, J., Zhou, B., de Groot, K., (1997) J. Mater. Sci.: Mater. Med., 8, p. 543; 
Mucalo, M.R., Yokogawa, Y., Toryiama, M., Suzuki, T., Kawamoto, Y., Nagata, F., Nishizawa, K., (1995) J. Mater. Sci.: Mater. Med., 6, p. 597; 
Mucalo, M.R., Yokogawa, Y., Suzuki, T., Kawamoto, Y., Nagata, F., Nishizawa, K., (1995) J. Mater. Sci.: Mater. Med., 6, p. 658; 
Granja, P.L., Pouys{\'e}gu, L., P{\'e}traud, M., De J{\'e}so, B., Baquey, C., Barbosa, M.A., (2001) J. Appl. Polym. Sci., 82, p. 3341; 
Granja, P.L., Pouys{\'e}gu, L., Deffieux, D., Daud{\'e}, G., De J{\'e}so, B., Labrug{\`e}re, C., Baquey, C., Barbosa, M.A., (2001) J. Appl. Polym. Sci., 82, p. 3354; 
Granja, P.L., Barbosa, M.A., Pouys{\'e}gu, L., De J{\'e}so, B., Baquey, C., (1999) Frontiers in Biomedical Polymers Applications, 2, p. 195. , Ottenbrite R. (Ed), Technomic Press, Lancaster; 
Granja, P.L., Pouys{\'e}gu, L., De J{\'e}so, B., Rouais, F., Baquey, C., Barbosa, M.A., (2001) J. Mater. Sci., 36, p. 2163; 
Kim, S.S., Jeong, W.Y., Shin, B.C., Oh, S.Y., Kim, H.W., Rhee, J.M., (1998) J. Biomed. Mater. Res., 40, p. 401; 
Saito, T., Arsenault, A.L., Yamauchi, M., Kuboki, Y., Crenshaw, M.A., (1997) Bone, 21, p. 305; 
van den Bos, T., Beertsen, W., (1994) J. Bone Min. Res., 9, p. 1205; 
Linde, A., (1992) Calcification in Biological Systems, p. 269. , Bonucci E. (Ed), CRC Press, Boca Raton; 
Veis, A., Sharkey, M., Dickson, I., (1977) Calcium Binding Proteins and Calcium Function, p. 408. , Wasserman R.H. (Ed), Elsevier, New York; 
Vilamitjana-Am{\'e}d{\'e}e, J., Bareille, R., Rouais, F., Caplan, A.I., Harmand, M.F., (1993) In Vitro Cell. Dev. Biol., 29 A, p. 699; 
Mosmann, T., (1983) J. Immunol. Meth., 65, p. 55; 
Dumitriu, S., Dumitriu, M., (1996) Polysaccharides in Medicinal Applications, p. 705. , Dumitriu S. (Ed), Marcel Dekker, New York; 
Van Blitterswijk, C.A., Bakker, D., Hesseling, S.C., Koerten, H.K., (1991) Biomaterials, 12, p. 187; 
Curtis, A.S.G., Forrester, J.V., Clark, P., (1986) J. Cell. Sci., 86, p. 9; 
Katayama, Y., House, C.M., Udagawa, N., Kazama, J.J., McFarland, R.J., Martin, T.J., Findlay, D.M., (1998) J. Cell. Physiol., 176, p. 179; 
Boskey, A.J., (1995) Ann. N. Y. Acad. Sci., 760, p. 249; 
Yamamoto, M., Kato, K., Ikada, Y., (1997) J. Biomed. Mater. Res., 37, p. 29; 
Alliot-Licht, B., Gregoire, M., Orly, I., Menanteau, J., (1991) Biomaterials, 12, p. 752; 
Oreffo, R.O.C., Driessens, F.C.M., Planell, J.A., Triffitt, J.T., (1998) Biomaterials, 19, p. 1845; 
Sun, J.-S., Tsuang, Y.-H., Liao, C.-J., Liu, H.-C., Hang, Y.-S., Lin, F.-H., (1997) J. Biomed. Mater. Res., 37, p. 324; 
Padilha, P.M., Tocha, J.C., Moreira, J.C., Campos, J.T.S., Federici, C.C., (1997) Talanta, 41, p. 317; 
Mizusawa, Y., Burke, J.R., (1996) J. Pediatr. Child. Health, 32, p. 350; 
Anwar, M., Krishnamoorthy, S., (1987) Indian J. Text. Res., 12, p. 24; 
Hoffman, A.S., (2004) Biomaterials Science: An Introduction to Materials in Medicine. second ed., p. 107. , Ratner B.D., Hoffman A.S., Schoen F.J., and Lemons J.F. (Eds), Elsevier-Academic Press, San Diego
},
	month = {2006///},
	pages = {728 - 739},
	abstract = {Grafting of phosphate groups has been investigated as a means to render biomedical polymers able to induce the formation of an apatite layer. In the present investigation, regenerated cellulose hydrogels were surface modified by phosphorylation using a previously reported method. The in vitro biocompatibility of materials with varying phosphate contents was evaluated in cultured human bone marrow stromal cells (HBMSC), in terms of cytotoxicity, cell attachment, proliferation and immunohistochemistry. Negatively charged cellulose phosphates were neutralized in the form of the calcium salt before contact with cells. Cellulose phosphates were not cytotoxic, independently of the phosphate content. Unmodified regenerated cellulose hydrogels showed good rates of HBMSC attachment and proliferation. On the contrary, negatively charged, highly hydrophilic, phosphorylated surfaces showed poor HBMSC attachment and proliferation, as well as poor alkaline phosphatase-specific activity and expression of osteocalcin and type I collagen. Differences found are discussed in terms of surface chemical functionality, hydrophilicity and charge. {\textcopyright} 2005 Elsevier B.V. All rights reserved.},
	keywords = {Biocompatibility, Biomaterials, Cell attachment, Cell culture, Cellulose, Cellulose derivatives, Cellulose phosphates, Cytotoxicity, Grafting (chemical), Human bone marrow stromal cells, Hydrogels, hydrophilicity, immunohistochemistry, In vitro, Phosphorylation, Proliferation, Regenerated cellulose hydrogels, toxicity},
	isbn = {13815148 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33745266092\&partnerID=40\&md5=52325c348b6bd4139bbfb3dbc0419bd8},
	author = {Granja, P.L. and J{\'e}so, B.D. and Bareille, R. and Rouais, F. and Baquey, C. and Barbosa, M.A.}
}
@conference {2388,
	title = {Characterization of fetal heart rate irregularity using approximate entropy and wavelet filtering},
	booktitle = {Computers in CardiologyComput Cardiol},
	series = {2006 Computers in Cardiology, CIC},
	volume = {33},
	year = {2006},
	note = {Conference code: 73228Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusArt. No.: 4512006CODEN: COCADLanguage of Original Document: EnglishCorrespondence Address: Marques De S{\'a}, J. P.; Faculdade de Engenharia, DEEC, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; email: jmsa@fe.up.ptReferences: Pincus, S., Approximate entropy as a measure of system complexity (1991) Proc. Natl. Acad. Sci. U.S.A, 88, pp. 2297-2301;Pincus, S., Randomness and degrees of irregularity (1996) Proc. Natl. Acad. Sci. U.S.A, 93, pp. 2083-2088; 
Pincus, S.M., Viscarello, R.R., Approximate entropy: A regularity measure for fetal heart rate (1992) Obstet. Gynecol, 2, pp. 249-255; 
Fleisher, L.A., Dipietro, J.A., Johnson, T.R., Pincus, S., Complementary and non-coincident increases in heart rate variability and irregularity during fetal development (1997) Clin. Sci, 4, pp. 345-349; 
van Leeuwen, P., Lange, S., Betterman, H., Gronemeyer, D., Hatzmann, W., Fetal heart rate variability and complexity in the course of pregnancy (1999) Early Human Develop, 54, pp. 259-269; 
Pincus, S., Assessing Serial Irregularity and its Implications for Health (2001) Ann N Y Acad Sci, 954, pp. 245-267; 
Cysarz, D., Betterman, H., van Leeuwen, P., Entropies of short binary sequences in heart period dynamics (2000) Am J Physiol Heart Circ Physiol, 6, pp. 2163-2172; 
Signorini, M.G., Magenes, G., Cerutti, S., Arduini, D., Linear and Nonlinear Parameters for the Analysis of Fetal Heart Rate Signal from Cardiotocographic Recordings (2003) IEEE Tr. BME, 50, pp. 365-373; 
Ayres-de-Campos, D., Bernardes, J., Garrido, A., Marques-de-S{\'a}, J., Pereira-Leite, L., SisPorto 2.0 - A Program for Automated Analysis of Cardiotocograms (2000) J Matern Fetal Med, 9, pp. 311-318; 
S{\'a} Couto, P.M., van Meurs, W.L., Bernardes, J.F., Marques de, S.J., Goodwin, J.A., Combined Model-Driven and Rule-Based Approach for Educational Simulation of Fetal Heart Rate (2002) Proc. 16th Biennial Int. Conf. Europ. Assoc. for Speech, Signal and Image Processing, pp. 400-402. , EURASIP, Brno, Czech Rep, pp; 
Marques de S{\'a}, J.P., Characterization of fetal heart rate using approximate entropy (2005) Proc. IEEE Comp. in Cardiology 2005, 32, pp. 671-673
},
	month = {2006///},
	pages = {933 - 936},
	address = {Valencia},
	abstract = {Approximate Entropy (ApEn) has been used by several researchers as a means to characterize the irregularity of fetal heart rate (FHR), a major contributor to fetal risk assessment. The present work starts by analyzing the influence of accelerative and decelerative (AD) components of the FHR signal in the computation of ApEn. Having shown that the AD components may completely distort the ApEn evaluation we proceed to present two methods of eliminating the influence of the AD components: wavelet filtering; AD detection using specific algorithms based on well-established physiological criteria. We obtained good results with the first method when applied to simulated signals; however it proved unreliable in the practical application of discriminating normal from pathological fetal FHR. With the second method we were able to achieve this discrimination with high statistical significance.},
	keywords = {Approximate entropy, Cardiology, Fetal heart rate, Neonatal monitoring, Occupational risks, Physiological models, risk assessment, Risk management, Safety engineering, Simulated signals, Statistical significance, Wavelet filtering},
	isbn = {02766574 (ISSN); 1424425328 (ISBN); 9781424425327 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-50149100681\&partnerID=40\&md5=51e2dd8109d66e30498d7c2f8689c820},
	author = {Magalh{\~a}es, F. and Marques De S{\'a}, J.P. and Bernardes, J. and Ayres-de-Campos, D.}
}
@book {2398,
	title = {The class imbalance problem in TLC image classification},
	series = {Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)Lect. Notes Comput. Sci.},
	volume = {4142 LNCS},
	year = {2006},
	note = {Conference code: 68310Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Sousa, A.V.; Instituto Superior de Engenharia do Porto, Rua Dr. Antonio Bernardino de A. 431, 4200-072 Porto, Portugal; email: ats@isep.ipp.ptReferences: Kubat, M., Holte, R., Matwin, S., Learning when negative examples abound (1997) European Conference on Machine Learning, pp. 146-153;Lazarevi{\'c}, A., Srivastava, J., Kumar, V., Data mining for analysis of rare events: A case study in security, financial and medical applications (2004) PAKDD-2004; 
Skurichina, M., Raudys, S., Duin, R.P.W., K-nearest neighbors directed noise injection in multilayer perceptron training (2000) IEEE Transactions on Neural Networks, 11 (2); 
Chawla, N.V., Japkowicz, N., Kolcz, A., Editorial: Special issue on learning from imbalanced data sets (2004) Sigkdd Explorations, 6 (1), pp. 1-6; 
Japkowicz, N., Class imbalances: Are we focusing on the right issue? Workshop on Learning from Imbalanced Datasets II, p. 6. , (2003) ICML, Washington DC; 
Chawla, N.V., Lazarevi{\'c}, A., Hall, L.O., Bowyer, K.W., SMOTEBoost: Improving the prediction of minority class in boosting (2003) 7th European Conference on Principles and Practice of Knowledge Discovery in Databases (PKDD), pp. 107-119; 
Domingos, P., MetaCost: A general method for making classifiers cost-sensitive (1999) Conference on Knowledge Discovery in Data Proceedings of the Fifth ACM SIGKDD International Conference on Knowledge Discovery and Data Mining, pp. 155-164; 
Fan, W., Stolfo, S., Zhang, J., Chan, P., Adacost: Misclassication cost-sensitive boosting (1999) Proceedings of Sixteenth International Conference on Machine Learning, pp. 983-990; 
Tax, D.M.J., One-class classification; Concept-learning in the absence of counterexamples (2001) ASCI Dissertation Series, 65, pp. 1-190. , PhD thesis Delft University of Technology; 
Ridder, D., Tax, D.M.J., Duin, R.P.W., An experimental comparison of one-class classification methods (1998) Proc ASCI{\textquoteright}98, 4th Annual Conf of the Advanced School for Computing and Imaging, pp. 213-218. , (Lommel, Belgium, June 9-11), ASCI, Delft; 
Lazarevic, A., Ertoz, L., Ozgur, A., Srivastava, J., Kumar, V., A comparative study of anomaly detection schemes in network intrusion detection (2003) Proceedings of Third SIAM Conference on Data Mining, , San Francisco; 
Weiss, G.M., Mining with rarity: A unifying framework (2004) Sigkdd Explorations, 6 (1), pp. 7-19; 
Lewis, D.D., Gale, W.A., A sequential algorithm for training text classifiers (1994) Proceedings of International ACM Conference on Research and Development in Information Retrieval, pp. 3-12; 
Visa, S., Ralescu, A., Learning imbalanced and overlapping classes using fuzzy sets (2003) Workshop on Learning from Imbalanced Datasets II, , ICML, Washington DC; 
Sousa, A.V., Aguiar, R., Mendon{\c c}a, A.M., Campilho, A., Automatic lane and band detection in images of thin layer chromatography (2004) LNCS, 3212. , Springer; 
Sousa, A.V., Mendon{\c c}a, A.M., Campilho, A., Aguiar, R., Miranda, C.S., Feature extraction for classification of thin-layer chromatography images (2005) LNCS, 3656. , Springer; 
Japkowicz, N., Learning from imbalanced data sets: A comparison of various strategies (2000) Proceedings of Learning from Imbalanced Data, pp. 10-15; 
Kubat, M., Holte, R., Matwin, S., Machine learning for the detection of oil spills in satellite radar images (1998) Machine Learning, 30, pp. 195-215; 
Ha, T., Bunke, H., Off-Line, handwritten numeral recognition by perturbation method (1997) Pattern Analysis and Machine Intelligence, 19 (5), pp. 535-539; 
Lee, S., Noisy replication in skewed binary classification (2000) Computational Statistics \& Data Analysis; 
Heijden, F., Robert, P.W.D., Ridder, D., Tax, D.M.J., (2004) Classification, Parameter Estimation and State Estimation, , Wiley; 
Demant, C., Streicher-Abel, B., Waszkewitz, P., (1999) Industrial Image Processing, , Springer-Verlag; 
Chawla, N.V., Bowyer, K., Hall, L., Kegelmeyer, W., SMOTE: Synthetic minority over-sampling technique (2002) Jounal of Artificial Intelligence Research, 16, pp. 321-357
},
	month = {2006///},
	pages = {513 - 523},
	address = {Povoa de Varzim},
	abstract = {The paper presents the methodology developed to solve the class imbalanced problem that occurs in the classification of Thin-Layer Chromatography (TLC) images. The proposed methodology is based on resampling, and consists in the undersampling of the majority class (normal class), while the minority classes, which contain Lysosomal Storage Disorders (LSD) samples, are oversampled with the generation of synthetic samples. For image classification two approaches are presented, one based on a hierarchical classifier and another uses a multiclassifier system, where both classifiers are trained and tested using balanced data sets. The results demonstrate a better performance of the multiclassifier system using the balanced sets. {\textcopyright} Springer-Verlag Berlin Heidelberg 2006.},
	keywords = {Chromatographic analysis, Class imbalanced problem, Classification (of information), Hierarchical systems, Lysosomal Storage Disorders (LSD), Medical imaging, Multiclassifier system, Problem solving, Thin-Layer Chromatography (TLC) images},
	isbn = {03029743 (ISSN); 3540448942 (ISBN); 9783540448945 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33749667604\&partnerID=40\&md5=3b4a717035de29f4bed59a04613836cd},
	author = {Sousa, A.V. and Mendon{\c c}a, A.M. and Campilho, A.}
}
@article {2415,
	title = {Cultivation of human embryonic stem cells without the embryoid body step enhances osteogenesis in vitro},
	journal = {Stem CellsStem Cells},
	volume = {24},
	year = {2006},
	note = {Cited By (since 1996): 80Export Date: 21 November 2012Source: ScopusCODEN: STCEEdoi: 10.1634/stemcells.2005-0383Language of Original Document: EnglishCorrespondence Address: Langer, R.S.; Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA 02139-4307, United States; email: rlanger@mit.eduChemicals/CAS: apatite, 64476-38-6; calcium, 7440-70-2; collagen, 9007-34-5; dexamethasone, 50-02-2; hydroxyapatite, 1306-06-5, 51198-94-8; phosphorus, 7723-14-0; alkaline phosphatase, 9001-78-9; calcium phosphate, 10103-46-5, 13767-12-9, 14358-97-5, 7758-87-4; osteocalcin, 136461-80-8; Alkaline Phosphatase, 3.1.3.1; Calcium Phosphates; Collagen, 9007-34-5; Osteocalcin, 104982-03-8; calcium phosphate, 10103-46-5References: Langer, R., Vacanti, J.P., Tissue engineering (1993) Science, 260, pp. 920-926;Alsberg, E., Anderson, K.W., Albeiruti, A., Cell-interactive alginate hydrogels for bone tissue engineering (2001) J Dent Res, 80, pp. 2025-2029; 
Bhatia, M., Derivation of the hematopoietic stem cell compartment from human embryonic stem cell lines (2005) Ann N Y Acad Sci, 1044, pp. 24-28; 
Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S., Embryonic stem cell lines derived from human blastocysts (1998) Science, 282, pp. 1145-1147. , erratum in Science 1998 282:1827; 
Perlingeiro, R.C., Kyba, M., Daley, G.Q., Clonal analysis of differentiating embryonic stem cells reveals a hematopoietic progenitor with primitive erythroid and adult lymphoid-myeloid potential (2001) Development, 128, pp. 4597-4604; 
Zur Nieden, N.I., Kempka, G., Ahr, H.J., In vitro differentiation of embryonic stem cells into mineralized osteoblasts (2003) Differentiation, 71, pp. 18-27; 
Bourne, S., Polak, J.M., Hughes, S.P., Osteogenic differentiation of mouse embryonic stem cells: Differential gene expression analysis by cDNA microarray and purification of osteoblasts by cadherin-11 magnetically activated cell sorting (2004) Tissue Eng, 10, pp. 796-806; 
Buttery, L.D., Bourne, S., Xynos, J.D., Differentiation of osteoblasts and in vitro bone formation from murine embryonic stem cells (2001) Tissue Eng, 7, pp. 89-99; 
Shimko, D.A., Burks, C.A., Dee, K.C., Comparison of in vitro mineralization by murine embryonic and adult stem cells cultured in an osteogenic medium (2004) Tissue Eng, 10, pp. 1386-1398; 
Bronson, S.K., Bone nodule formation via in vitro differentiation of murine embryonic stem cells (2003) Methods Enzymol, 365, pp. 241-251; 
Sottile, V., Thomson, A., McWhir, J., In vitro osteogenic differentiation of human ES cells (2003) Cloning Stem Cells, 5, pp. 149-155; 
Bielby, R.C., Boccaccini, A.R., Polak, J.M., In vitro differentiation and in vivo mineralization of osteogenic cells derived from human embryonic stem cells (2004) Tissue Eng, 10, pp. 1518-1525; 
Cao, T., Heng, B.C., Ye, C.P., Osteogenic differentiation within intact human embryoid bodies result in a marked increase in osteocalcin secretion after 12 days of in vitro culture, and formation of morphologically distinct nodule-like structures (2005) Tissue Cell, 37, pp. 325-334; 
Ginis, I., Luo, Y., Miura, T., Differences between human and mouse embryonic stem cells (2004) Dev Biol, 269, pp. 360-380; 
Itskovitz-Eldor, J., Schuldiner, M., Karsenti, D., Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers (2000) Mol Med, 6, pp. 88-95; 
Keller, G.M., In vitro differentiation of embryonic stem cells (1995) Curr Opin Cell Biol, 7, pp. 862-869; 
Yamada, T., Yoshikawa, M., Kanda, S., In vitro differentiation of embryonic stem cells into hepatocyte-like cells identified by cellular uptake of indocyanine green (2002) Stem Cells, 20, pp. 146-154; 
Aubin, J.E., Osteoprogenitor cell frequency in rat bone marrow stromal populations: Role for heterotypic cell-cell interactions in osteoblast differentiation (1999) J Cell Biochem, 72, pp. 396-410; 
Sarugaser, R., Lickorish, D., Baksh, D., Human umbilical cord perivascular (HUCPV) cells: A source of mesenchymal progenitors (2005) Stem Cells, 23, pp. 220-229; 
Komori, T., Yagi, H., Nomura, S., Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts (1997) Cell, 89, pp. 755-764; 
Bonewald, L.F., Harris, S.E., Rosser, J., Von Kossa staining alone is not sufficient to confirm that mineralization in vitro represents bone formation (2003) Calcif Tissue Int, 72, pp. 537-547; 
Amit, M., Carpenter, M.K., Inokuma, M.S., Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture (2000) Dev Biol, 227, pp. 271-278; 
Karp, J.M., Shoichet, M.S., Davies, J.E., Bone formation on two-dimensional poly(DL-lactide-co-glycolide) (PLGA) films and three-dimensional PLGA tissue engineering scaffolds in vitro (2003) J Biomed Mater Res, 64 A, pp. 388-396; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats (1988) Cell Tissue Res, 254, pp. 317-330; 
Davies, J.E., In vitro modeling of the bone/implant interface (1996) Anat Rec, 245, pp. 426-445; 
Slizova, D., Krs, O., Pospisilova, B., Alternative method of rapid drying vascular specimens for scanning electron microscopy (2003) J Endovasc Ther, 10, pp. 285-287; 
Purpura, K.A., Aubin, J.E., Zandstra, P.W., Two-color image analysis discriminates between mineralized and unmineralized bone nodules in vitro (2003) Biotechniques, 34, pp. 1188-1192. , 1194, 1196 passim; 
Purpura, K.A., Aubin, J.E., Zandstra, P.W., Sustained in vitro expansion of bone progenitors is cell density dependent (2004) Stem Cells, 22, pp. 39-50; 
Klimanskaya, I., Chung, Y., Meisner, L., Human embryonic stem cells derived without feeder cells (2005) Lancet, 365, pp. 1636-1641; 
Draper, J.S., Pigott, C., Thomson, J.A., Surface antigens of human embryonic stem cells: Changes upon differentiation in culture (2002) J Anat, 200, pp. 249-258; 
Aubin, J.E., Liu, F., Malaval, L., Osteoblast and chondroblast differentiation (1995) Bone, 17, pp. 77S-83S; 
Aubin, J.E., Bone stem cells (1998) J Cell Biochem Suppl, 30-31, pp. 73-82; 
Schmitt, B., Ringe, J., Haupl, T., BMP2 initiates chondrogenic lineage development of adult human mesenchymal stem cells in high-density culture (2003) Differentiation, 71, pp. 567-577; 
Lian, J.B., McKee, M.D., Todd, A.M., Induction of bone-related proteins, osteocalcin and osteopontin, and their matrix ultrastructural localization with development of chondrocyte hypertrophy in vitro (1993) J Cell Biochem, 52, pp. 206-219; 
Wei, C.L., Miura, T., Robson, P., Transcriptome profiling of human and murine ESCs identifies divergent paths required to maintain the stem cell state (2005) Stem Cells, 23, pp. 166-185; 
Heng, B.C., Cao, T., Stanton, L.W., Strategies for directing the differentiation of stem cells into the osteogenic lineage in vitro (2004) J Bone Miner Res, 19, pp. 1379-1394
},
	month = {2006///},
	pages = {835 - 843},
	abstract = {Osteogenic cultures of embryonic stem cells (ESCs) are predominately derived from three-dimensional cell spheroids called embryoid bodies (EBs). An alternative method that has been attempted and merits further attention avoids EBs through the immediate separation of ESC colonies into single cells. However, this method has not been well characterized and the effect of omitting the EB step is unknown. Herein, we report that culturing human embryonic stem cells (hESCs) without the EB stage leads to a sevenfold greater number of osteogenic cells and to spontaneous bone nodule formation after 10-12 days. In contrast, when hESCs were differentiated as EBs for 5 days followed by plating of single cells, bone nodules formed after 4 weeks only in the presence of dexamethasone. Furthermore, regardless of the inclusion of EBs, bone matrix formed, including cement line matrix and mineralized collagen, which displayed apatitic mineral (PO                        4) with calcium-to-phosphorous ratios similar to those of hydroxyapatite and human bone. Together these results demonstrate that culturing hESCs without an EB step can be used to derive large quantities of functional osteogenic cells for bone tissue engineering. {\textcopyright}AlphaMed Press.
},
	keywords = {alkaline phosphatase, Apatite, article, bone development, bone growth, bone matrix, bone mineralization, Bone tissue engineering, Calcium, calcium phosphate, Calcium phosphates, Cell Culture Techniques, cell differentiation, cell separation, Collagen, collagen synthesis, culture technique, cytology, dexamethasone, embryo, embryo culture, Embryoid bodies, embryonic stem cell, extracellular matrix, human, human cell, human embryo, Human embryonic stem cells, Humans, hydroxyapatite, in vitro study, Matrix characterization, metabolism, methodology, ossification, osteocalcin, Osteogenesis, Osteogenic cell frequency, phosphorus, spheroid cell, Tissue engineering, totipotent stem cell, Totipotent Stem Cells},
	isbn = {10665099 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33745504422\&partnerID=40\&md5=214785ea9403886207b058cb87570338},
	author = {Karp, J.M. and Ferreira, L.S. and Khademhosseini, A. and Kwon, A.H. and Yeh, J. and Langer, R.S.}
}
@conference {2435,
	title = {Characterization of fetal heart rate using approximate entropy},
	booktitle = {Computers in CardiologyComput Cardiol},
	series = {Computers in Cardiology, 2005},
	volume = {32},
	year = {2005},
	note = {Conference code: 69149Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusArt. No.: 1588190CODEN: COCADdoi: 10.1109/CIC.2005.1588190Language of Original Document: EnglishCorrespondence Address: Marques de S{\'a}, J.P.; Faculdade de Engenharia, DEEC, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; email: jmsa@fe.up.ptReferences: Felgueiras, C., Marques de, S.J., Bernardes, J., Gama, S., Classification of Foetal Heart Rate Sequences Based on Fractal Features (1998) Medical \& Biological Engineering \& Computing, 2, pp. 197-201;Pincus, S., Approximate entropy as a measure of system complexity (1991) Proc. Natl. Acad. Sci. U.S.A, 88, pp. 2297-2301; 
Pincus, S.M., Viscarello, R.R., Approximate entropy: A regularity measure for fetal heart rate (1992) Obstet. Gynecol, 2, pp. 249-255; 
Pincus, S., Approximate Entropy (ApEn) as a Complexity Measure (1995) Chaos, 5, pp. 110-117; 
Pincus, S., Randomness and degrees of irregularity (1996) Proc. Natl. Acad. Sci. U.S.A, 93, pp. 2083-2088; 
Pincus, S., Kalman, R.E., Not all (possibly) "random" sequences are created equal (1997) Proc. Natl. Acad. Sci. U.S.A, 94, pp. 3513-3518; 
Fleisher, L.A., Dipietro, J.A., Johnson, T.R., Pincus, S., Complementary and non-coincident increases in heart rate variability and irregularity during fetal development (1997) Clin. Sci, 4, pp. 345-349; 
van Leeuwen, P., Lange, S., Betterman, H., Gronemeyer, D., Hatzmann, W., Fetal heart rate variability and complexity in the course of pregnancy (1999) Early Human Develop, 54, pp. 259-269; 
Pincus, S., Assessing Serial Irregularity and its Implications for Health (2001) Ann N Y Acad Sci, 954, pp. 245-267; 
Cysarz, D., Betterman, H., van Leeuwen, P., Entropies of short binary sequences in heart period dynamics (2000) Am J Physiol Heart Circ Physiol, 6, pp. 2163-2172; 
Ayres-de-Campos, D., Bernardes, J., Garrido, A., Marques-de-S{\'a}, J., Pereira-Leite, L., SisPorto 2.0 - A Program for Automated Analysis of Cardiotocograms (2000) J Matern Fetal Med, 9, pp. 311-318
},
	month = {2005///},
	pages = {671 - 673},
	address = {Lyon},
	abstract = {Approximate entropy (ApEn) is an interesting measure for assessing the irregularity of time series and in particular for characterizing heart rate variability. In the present paper we apply ApEn to the characterization of three patterns of fetal heart rate (FHR): calm sleep; calm vigilance; pathological flat-sinusoidal condition. We conclude that ApEn can perfectly discriminate the pathological FS pattern from the normal A and C patterns and is far better for FHR pattern discrimination than classic time-domain variability indexes used in clinical practice. {\textcopyright} 2005 IEEE.},
	keywords = {Approximate entropy (ApEn), Cardiology, Fetal heart rate, Fetal monitoring, Heart rate variability, Pathology, Sleep research, Time domain analysis},
	isbn = {02766574 (ISSN); 0780393376 (ISBN); 9780780393370 (ISBN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33847143708\&partnerID=40\&md5=9c0b28217d5f8a40e2e1741bba8c4561},
	author = {Marques De S{\'a}, J.P.}
}
@article {2438,
	title = {Chemical modification of chitosan by phosphorylation: An XPS, FT-IR and SEM study},
	journal = {Journal of Biomaterials Science, Polymer EditionJ. Biomater. Sci. Polym. Ed.},
	volume = {16},
	year = {2005},
	note = {Cited By (since 1996): 50Export Date: 21 November 2012Source: ScopusCODEN: JBSEEdoi: 10.1163/156856205774576736Language of Original Document: EnglishCorrespondence Address: Amaral, I.F.; Laborat{\'o}rio de Biomateriais, INEB - Instituto de Engenharia Biom{\'e}dica, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: iamaral@ineb.up.ptChemicals/CAS: chitosan, 9012-76-4; phosphate, 14066-19-4, 14265-44-2; apatite, 64476-38-6; butanol, 35296-72-1, 71-36-3; calcium ion, 14127-61-8; Chitosan, 9012-76-4; PhosphatesReferences: Khor, E., (2001) Chitin: Fulfilling a Biomaterials Promise, , Elsevier Science, Oxford;Vande Vord, P.J., Matthew, H.W.T., DeSilva, S.P., Mayton, L., Wu, B., Wooley, P.H., (2002) J. Biomed. Mater. Res., 59, p. 585; 
Muzzarelli, R.A.A., Mattioli-Belmonte, M., Tietz, C., Biagini, R., Ferioli, G., Brunelli, M.A., Fini, M., Biagini, G., (1994) Biomaterials, 15, p. 1075; 
Lee, J.Y., Nam, S.H., Im, S.Y., Park, Y.J., Lee, Y.M., Seol, Y.J., Chung, C.P., Lee, S.J., (2002) J. Control. Rel., 78, p. 187; 
(2002) Chitosan in Pharmacy and Chemistry, p. 233. , R.A. A. Muzzarelli and C. Muzzarelli (Eds), in: Atec, Grottammare; 
Zhao, F., Yin, Y.J., Lu, W.W., Leong, J.C., Zhang, W.J., Zhang, J.Y., Zhang, M.F., Yao, K.D., (2002) Biomaterials, 23, p. 3227; 
Tanahashi, M., Matsuda, T., (1997) J. Biomed Mater. Res., 34, p. 305; 
Yokogawa, Y., Reyes, J.P., Mucalo, M.R., Toriyama, M., Kawamoto, Y., Suzuki, T., Nishizawa, K., Kamayama, T., (1997) J. Mater. Sci. Mater. Med., 8, p. 407; 
Varma, H.K., Yokogawa, Y., Espinosa, F.F., Kawamoto, Y., Nishizawa, K., Nagata, F., Kameyama, T., (1999) Biomaterials, 20, p. 879; 
Orban, J.M., Marra, K.G., Hollinger, J.O., (2002) Tissue Eng., 8, p. 529; 
Ilyina, A.V., Varlamov, V.P., Tikhonov, V.E., Yamskov, I.A., Davankov, V.A., (1994) Biotechnol. Appl. Biochem., 19, p. 199; 
Sugihara, J., Imamura, T., Yanase, T., Yamada, H., Imoto, T., (1982) J. Chromatogr., 229, p. 193; 
Kennedy, J.F., Cabral, J.M.S., (1983) Solid Phase Biochemistry: Analytical Synthetic Aspects, p. 253. , in: W. H. Schouten (Ed.) Wiley, New York, NY; 
Chatelet, C., Damour, O., Domard, A., (2001) Biomaterials, 22, p. 261; 
Granja, P.L., Pouysegu, L., Petraud, M., De Jeso, B., Baquey, C., Barbosa, M.A., (2001) J. Appl. Polym. Sci., 82, p. 3341; 
Fricain, J.C., Granja, P.L., Barbosa, A.M., de Jeso, B., Barthe, N., Baquey, C., (2002) Biomaterials, 23, p. 971; 
Sakairi, N., Shirai, A., Miyazaki, S., Tashiro, H., Tsuji, Y., Kawahara, H., Yoshida, T., Tokura, S., (1998) Kobunshi Ronbunshu, 55, p. 212; 
Sakaguchi, T., Horkoshi, T., Nakajima, A., (1981) Agricult. Biol. Chem., 45, p. 2191; 
Nishi, N., Ebina, A., Nishimura, S., Tsutsumi, A., Hasegawa, O., Tokura, S., (1986) Int. J. Biol. Macromol., 8, p. 311; 
Baxter, B., Dillon, M., Taylor, K.D.A., Roberts, G.A.F., (1992) Int. J. Biol. Macromol., 14, p. 166; 
Wang, W., Bo, S.Q., Li, S.Q., Qin, W., (1991) Int. J. Biol. Macromol., 13, p. 281; 
Aiba, S., (1986) Int. J. Biol. Macromol., 8, p. 173; 
Hirano, S., Ohe, Y., Ono, H., (1976) Carbohydr. Res., 47, p. 315; 
Shigemasa, Y., Matsuura, H., Sashiwa, H., Saimoto, H., (1996) Int. J. Biol. Macromol., 18, p. 237; 
Seah, M.P., (1990) Practical Surface Analysis, 1, p. 541. , D.in: Briggs and M. P. Seah (Eds) Wiley, Chichester; 
Sherwood, P.M.A., (1990) Practical Surface Analysis, 1, p. 555. , in: D. Briggs and M. P. Seah (Eds) Wiley, Chichester; 
Hudson, S.M., Smith, C., (1998) Bio-polymers from Renewable Resources, p. 96. , in: D. L. Kaplan (Ed.) Springer, Berlin; 
Shepherd, R., Reader, S., Falshaw, A., (1997) Glycoconj. J., 14, p. 535; 
Wang, X.H., Ma, J.B., Wang, Y.N., He, B.L., (2001) Biomaterials, 22, p. 2247; 
Lee, Y.M., Shin, E.M., (1991) J. Membr. Sci., 64, p. 145; 
Kim, S.S., Jeong, W.Y., Shin, B.C., Oh, S.Y., Kim, H.W., Rhee, J.M., (1998) J. Biomed. Mater. Res., 40, p. 401; 
Whistler, R.L., Towle, G.A., (1969) Arch. Biochem. Biophys., 135, p. 396; 
Gardner, S.D., Singamsetty, C.S.K., Booth, G.L., He, G., (1995) Carbon, 33, p. 587; 
Briggs, D., (1990) Practical Surface Analysis, 1, p. 437. , in: D. Briggs and M. P. Seah (Eds) Wiley, Chichester; 
Beamson, G.D.B., (1992) High Resolution XPS of Organic Polymers, , Wiley, Chichester; 
Ratner, B.D., Caster, D.G., (1997) Surface Analysis - The Principal Techniques, p. 43. , in: J. C. Vickerman (Ed.) Wiley, Chichester; 
Bourbigot, S., Lebras, M., Gengembre, L., Delobel, R., (1994) Appl. Surf. Sci., 81, p. 299; 
Kilpadi, D.V., Raikar, G.N., Liu, J., Lemons, J.E., Vohra, Y., Gregory, J.C., (1998) J. Biomed. Mater. Res., 40, p. 646; 
Landis, W.J., (1984) Vac. Technol., A2, p. 1108; 
Wagner, C.D., Naumkin, A.V., Kraut-Vass, A., Alisson, J.W., Powell, C.J., Rumble Jr., J.R., (2003) NIST-X-ray Photoelectron Spectroscopy Database 20 Version 3.4, , http://srdata.nist.gov/xps/, National Institute of Standards and Technology, Gaithersburg, MD Available online at; 
Quan, D.T., Le Bloa, A., Habib, H., Bonnaud, O., Meinnel, J., Quemerais, A., Marchand, R., (1989) Rev. Phys. Appl., 24, p. 545; 
Lindberg, B., Maripuu, R., Siegbahn, K., Larsson, R., Golander, C.G., Eriksson, J.C., (1983) J. Colloid Interf. Sci., 95, p. 308; 
Domard, A., (1997) Advances in Chitin Science, 2, p. 410. , in: A. Domard, G. Roberts and K. V{\r a}rum (Eds) Jacques Andr{\'e}, Lyon; 
Granja, P.L., Pouysegu, L., Deffieux, D., Daude, G., De Jeso, B., Labrugere, C., Baquey, C., Barbosa, M.A., (2001) J. Appl. Polym. Sci., 82, p. 3354; 
Roeges, N.P.G., (1994) A Guide to the Complete Interpretation of Infrared Spectra of Organic Structures, , Wiley, Chichester; 
Pearson, F.G., Marchessault, R.H., Liang, C.Y., (1960) J. Polym. Sci., 43, p. 101; 
Roberts, G.A.F., (1992) Chitin Chemistry, , Macmillan, London; 
Kuptsov, A.H., Zhizhin, G.N., (1998) Handbook of Fourrier Transform Raman and Infrared Spectra of Polymers, , Elsevier, Amsterdam; 
Daasch, L.W., Smith, D.C., (1951) Anal. Chem., 23, p. 854; 
Bueno, W.A., (1990) Manual De Espectroscopia Vibracional, , McGraw-Hill, S{\~a}o Paulo; 
Nyquist, R.A., Craver, C.D., (1997) The Coblentz Society Desk Book of Infrared Spectra, , The Coblentz Society, French Village, MO; 
Hirano, S., Tobetto, K., Noishiki, Y., (1981) J. Biomed. Mater. Res., 15, p. 903
},
	month = {2005///},
	pages = {1575 - 1593},
	abstract = {In the present work, the surface of chitosan membranes was modified using a phosphorylation method carried out at room temperature. Phosphorylation may be of particular interest in materials for orthopaedic applications, due to the cation-exchange properties of phosphate functionalities. Phosphate groups chelate calcium ions, thus inducing the deposition of an apatite-like layer known to improve the osteoconduction of polymer-based implants. Additionally, the negatively charged phosphate functionalities, together with the positively charged amine groups from chitosan, are expected to provide chitosan with an amphoteric character, which may be useful as a combinatorial therapeutic strategy, by simultaneously allowing the immobilization of signalling molecules like growth factors. Phosphorylation was carried out at room temperature using the H                        3 PO
                        4/Et
                        3 PO
                        4/P
                        2O
                        5/butanol method. Surface characterization was performed by XPS, ATR-FT-IR, and SEM. Cross-sections were analyzed by SEM fitted with EDS. The phosphate content increased with the reaction time, as shown by XPS and ATR-FT-IR, a P/N atomic ratio of 0.73 being obtained after 48 h of treatment. High-resolution XPS spectra regarding C
                        1s, O
                        1s, N
                        1s and P
                        2p are discussed. The introduction of a neutralization step led to a reduction of P content, which pointed out to the presence of phosphates ionically bound to protonated amines, in addition to phosphate esters. EDS analysis of cross-sections revealed a gradual P reduction up to 50\% towards the inner part of the membrane. {\textcopyright} VSP 2005.
},
	keywords = {Acetylation, amine, animal, Animals, Apatite, article, bone conduction, butanol, calcium ion, cation exchange, cephalopod, chelate, Chemical modification, chemistry, Chitin, chitosan, comparative study, controlled study, Decapodiformes, Fourier transform infrared spectroscopy, Fourier transform infrared spectroscopy (FT-IR), Growth factor, immobilization, Implants (surgical), infrared spectroscopy, isolation and purification, Microscopy, Electron, Scanning, molecular weight, organophosphate, phosphate, Phosphate groups, Phosphates, Phosphorylation, Phosphorylation method, Plastics applications, priority journal, reflectometry, room temperature, Scanning electron microscopy, SEM-EDS, signal transduction, spectrometry, Spectrometry, X-Ray Emission, Spectroscopy, Fourier Transform Infrared, Surface chemical reaction, Surface Properties, surface property, temperature, time, Time Factors, viscosity, X ray photoelectron spectroscopy, X-rayphotoelectron spectroscopy (XPS)},
	isbn = {09205063 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33644875542\&partnerID=40\&md5=cb42a0fba6d2000d93b90af9724a5bc1},
	author = {Amaral, I.F. and Granja, P.L. and Barbosa, M.A.}
}
@article {2474,
	title = {Calcium phosphate-alginate microspheres as enzyme delivery matrices},
	journal = {BiomaterialsBiomaterials},
	volume = {25},
	year = {2004},
	note = {Cited By (since 1996): 92Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/j.biomaterials.2003.11.028PubMed ID: 15046927Language of Original Document: EnglishCorrespondence Address: Ribeiro, C.C.; Laborat{\'o}rio de Biomateriais, INEB, Inst. de Engenharia Biom{\'e}dica, Rua do Campo Alegre 823, Porto 4150-180, Portugal; email: cribeiro@ineb.up.ptChemicals/CAS: Alginates; alginic acid, 9005-32-7; calcium phosphate, 10103-46-5; Calcium Phosphates; Drug Carriers; Enzymes, Immobilized; Glucosylceramidase, EC 3.2.1.45; Glucuronic Acid, 576-37-4; Hexuronic Acids; PowdersReferences: Griffith, L.G., Naughton, G., Tissue engineering: Current challenges and expanding opportunities (2002) Science, 295, pp. 1009-1014;Hench, L.L., Polak, J.M., Third-generation biomedical materials (2002) Science, 295, pp. 1014-1017; 
Temenoff, J.S., Mikos, A.G., Injectable biodegradable materials for orthopedic tissue engineering (2000) Biomaterials, 21, pp. 2405-2412; 
Laurencin, C.T., Lu, H.H., Polymer-ceramic composites for bone tissue engineering (2000) Bone Engineering, pp. 463-472. , J.E. Davies. Toronto, Canada: EM Squared Inc; 
Gauthier, O., Bouler -., J.M., Weiss, P., Bosco, J., Daculsi, G., Aguado, E., Kinetic study of bone ingrowth and ceramic resorption associated with the implantation of different injectable calcium-phosphate bone substitutes (1999) J Biomed Mater Res, 47, pp. 28-35; 
Dupraz, A., Delecrin, J., Moreau, A., Pilet, P., Passut, N., Long term bone response to particulate injectable ceramic (1998) J Biomed Mater Res, 42, pp. 368-375; 
Grimandi, G., Weiss, P., Millot, F., Daculsi, G., In vitro evaluation of a new injectable calcium phosphate material (1998) J Biomed Mater Res, 39, pp. 660-666; 
Gauthier, O., Boix, D., Grimandi, G., Aguado, E., Bouler, J.M., Weiss, P., Daculsi, G., A new injectable phosphate biomaterial for immediate bone filling of extraction sockets: A preliminary study in dogs (1999) J Periodontol, 70, pp. 375-383; 
Peter, S.J., Nolley, J.A., Widmer, M.S., Mervin, J.E., Yaszemski, M.J., Yasko, A.W., Engel, P.S., Mikos, A.G., In vitro degradation of a poly (propylene fumarate)/β-tricalcium phosphate composite orthopedic scaffold (1997) Tissue Eng, 3, pp. 207-215; 
Sims, C.D., Butler, P.E.M., Casanova, R., Lee, B.T., Randolph, M.A., Lee, W.P.A., Vacant, C.A., Yamremchuk, M.J., Injectable cartilage using polyethylene oxide polymer substrates (1996) Plast Reconstr Surg, 98, pp. 843-850; 
Suggs, L.J., Shive, M.S., Garcia, C.A., Anderson, J.M., Mikos, A.G., In vitro cytotoxicity and in vivo biocompalibility of poly (propylene fumarate-co-ethylene glycol) hydrogels (1999) J Biomed Mater Res, 46, pp. 22-32; 
Suggs, L.J., Krishnan, R.S., Garcia, C.A., Peter, S.J., Anderson, J.M., Mikos, A.G., In vitro and in vivo degradation of poly(propylene fumarate-co-ethylene glycol) hydrogels (1998) J Biomed Mater Res, 42, pp. 312-320; 
Frazier, D.D., Lathi, V.K., Gerhart, T.N., Hayes, W.C., Ex vivo degradation of a poly (propylene glycol-fumarate) biodegradable particulate bone cement (1997) J Biomed Mater Res, 35, pp. 383-389; 
Kharas, G.B., Kamanetsky, M., Simantirakis, J., Beinlich, K.C., Rizzo, A.-.M.T., Caywood, G.A., Watson, K., Synthesis and characterization of fumarate-based polyesters for use in bioresorbable bone cement composites (1997) J Appl Polym Sci, 66, pp. 1123-1137; 
Paige, K.T., Cima, L.G., Yaremchuck, M.J., Vacant, J.P., Vacant, C.A., Injectable cartilage (1995) Plast Reconstr Surg, 96, pp. 1390-1400; 
Paige, K.T., Cima, L.G., Yaremchuck, M.J., Vacant, J.P., Vacant, C.A., De novo cartilage generation using calcium-alginate-chondrocyte constructs (1996) Plast Reconstr Surg, 97, pp. 168-180; 
Kulseng, B., Skjak-Braek, G., Ryan, L., Andersson, A., King, A., Faxvaag, A., Espevik, T., Transplantation of alginate microcapsules (1999) Transplantation, 67, pp. 978-984; 
Martinetti, R., Dolcini, L., Ravaglioli, A., Krajewski, A., Mangano, C., Experimental study on hydroxyapatite/N-carboxymethyl chitosan fillers (1997) Bioceramics, 10, pp. 503-506. , L. Sedel, \& C. Rey. Oxford, UK: Elsevier; 
Maruyama, M., Ito, M., In vitro properties of a chitosan-bonded self hardening paste with hydroxyapatite granules (1996) J Biomed Mater Res, 32, pp. 527-532; 
Dupraz, A., Nguyen, T.P., Richard, M., Daculsi, G., Passuti, N., Influence of a cellulosic ether carrier on the structure of biphasic calcium phosphate ceramic particles in an injectable composite material (1999) Biomaterials, 20, pp. 663-673; 
Ribeiro, C.C., Ma, B., Calcium-Titanium-Phosphate: A Potential Material to Be Used in the Biomedical Field, , submitted for publication; 
Alamo, J., Chemistry and properties of solids with NZP skeleton (1993) Solid State Ionics, 63, pp. 547-561; 
Takahiro, S., Motohiro, T., Hosono, H., Application of a microporous glass-ceramic with a skeleton of CaTi 4(PO 4) 6 to carriers for immobilization of enzymes (1991) J Ferment Bioeng, 72, pp. 384-391; 
Gross, U., Muller-Mai, C., Voigt, C., Mesgarian, M., Berger, G., Ploska, U., Tissue response in femur of rabbits after implantation of a new calcium titanium phosphate composition (2001) Key Eng Mater, 192-195, pp. 383-386; 
Otsuka, M., Matsuda, Y., Fox, J.L., Higuchi, W.I., Yu, D., Wong, J., A novel skeletal drug delivery system for anti bacterial drugs using self-setting hydroxyapatite cement (1990) Chem Pharm Bull (Tokyo), 38, pp. 3500-3502; 
Guicheux, J., Grimandi, G., Trecant, M., Faivre, A., Takahashi, S., Apatite as a carrier for growth hormone: In vitro characterization of loading and release (1997) J Biomed Mater Res, 34, pp. 165-170; 
Gombotz, W.R., Wee, S.F., Protein release from alginate matrices (1998) Adv Drug Deliv Rev, 31, pp. 267-285; 
Eiselt, P., Yeh, J., Latvala, R.K., Shea, L.D., Mooney, D.J., Porous carriers for biomedical applications based on alginate hydrogels (2000) Biomaterials, 21, pp. 1921-1927; 
Alsberg, V., Anderson, K., Albeiruti, A., Franceshi, R.T., Mooney, D.J., Cell interactive alginate hydrogels for bone tissue engineering (2001) J Dent Res, 80, pp. 2025-2029; 
Smidsrod, O., Draget, K.I., Chemistry and physical properties of alginates (1996) Carbohyd Europe, 14, pp. 6-13; 
Sivakumar, M., Manjubala, I., Panduranga Rao, K., Preparation, characterization and in-vitro release of gentamicin from coralline hydroxyapatite-chitosan composite microspheres (2002) Carbohyd Pol, 49, pp. 281-288; 
Qiu, Q.Q., Ducheyne, P., Ayyaswamy, P.S., New bioactive, degradable composite microspheres as tissue engineering substrates (2000) J Biomed Mater Res, 52, pp. 66-76; 
Hsu, F.Y., Chueh -., S.C., Wang, Y.J., Microspheres of hydroxyapatite/reconstituted collagen as supports for osteoblast cell growth (1999) Biomaterials, 20, pp. 1931-1936; 
Grabowski, G.A., Leslie, N., Wenstrup, R., Enzyme therapy for Gaucher disease: The first 5 years (1998) Blood Rev, 12, pp. 115-133; 
Xu, Y.H., Ponce, E., Sun, Y., Leonova, T., Bove, K., Witte, D., Grabowski, G.A., Turnover and distribution of intravenously administered mannose-terminated human acid beta glucosidase in murine and human tissues (1996) Pediatr Res, 39, pp. 313-322; 
Ferraz, M.P., Monteiro, F.J., Serro, A.P., Saramago, B., Gibson, I.R., Santos, J.D., Effect of chemical composition on hydrophobicity and zeta-potential of plasma spayed HA/CaO-P 2O 5 glass coatings (2001) Biomaterials, 22, pp. 3105-3112; 
Lopes, M.A., Monteiro, F.L., Santos, J.D., Serro, A.P., Saramago, B., Hydrophobicity, surface tension, and zeta potential measurements of glass reinforced hydroxyapatite composites (1999) J Biomed Mater Res, 45, pp. 370-375; 
Dupuy, B., Arien, A., Minnot, A.P., FT-IR membranes made with alginate/polylysine complexes. Variations with mannuronic or guluronic content of the polysaccharides. Art Cells, Blood Subs (1994) Immob Biotech, 20, pp. 71-82; 
Peters, T., (1996) All about Albumin: Biochemistry, Genetics and Medical Applications, , San Diego, CA: Academic Press Inc; 
Nakanishi, K., Sakiyama, T., Imamura, K., On the adsorption of proteins on solid surfaces, a common but very complicated phenomenon (2001) J Biosci Bioeng, 91, pp. 233-244
},
	month = {2004///},
	pages = {4363 - 4373},
	abstract = {The present study concerns the preparation and initial characterisation of novel calcium titanium phosphate-alginate (CTP-alginate) and hydroxyapatite-alginate (HAp-alginate) microspheres, which are intended to be used as enzyme delivery matrices and bone regeneration templates. Microspheres were prepared using different concentrations of polymer solution (1\% and 3\%w/v) and different ceramic-to-polymer solution ratios (0.1, 0.2 and 0.4w/w). Ceramic powders were characterised using X-ray diffraction, laser granulometry, Brunauer, Emmel and Teller (BET) method for the determination of surface area, zeta potential and Fourier transform infrared spectroscopy (FT-IR). Alginate was characterised using high performance size exclusion chromatography. The methodology followed in this investigation enabled the preparation of homogeneous microspheres with a uniform size. Studies on the immobilisation and release of the therapeutic enzyme glucocerebrosidase, employed in the treatment of Gaucher disease, were also performed. The enzyme was incorporated into the ceramic-alginate matrix before gel formation in two different ways: pre-adsorbed onto the ceramic particles or dispersed in the polymeric matrix. The two strategies resulted in distinct release profiles. Slow release was obtained after adsorption of the enzyme to the ceramic powders, prior to preparation of the microspheres. An initial fast release was achieved when the enzyme and the ceramic particles were dispersed in the alginate solution before producing the microspheres. The latter profile is very similar to that of alginate microspheres. The different patterns of enzyme release increase the range of possible applications of the system investigated in this work. {\textcopyright} 2003 Elsevier Ltd. All rights reserved.},
	keywords = {Absorption, Adsorption, Alginate, Alginates, article, Biomaterials, Bone, Calcium Compounds, Calcium phosphates, Calcium titanium phosphate, calcium titanium phosphate alginate, ceramics, Drug Carriers, Drug Delivery Systems, Enzyme delivery, Enzyme-delivery, Enzymes, Enzymes, Immobilized, Fourier transform infrared spectroscopy, Glucosylceramidase, Glucuronic Acid, Hexuronic Acids, High performance liquid chromatography, hydroxyapatite, hydroxyapatite alginate, laser, methodology, microsphere, Microspheres, Molecular Conformation, Particle Size, Powders, priority journal, surface property, unclassified drug, X ray diffraction},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-1642331315\&partnerID=40\&md5=217590793385cbaff039a6b260d3d85c},
	author = {Ribeiro, C.C. and Barrias, C.C. and Barbosa, M.A.}
}
@book {2486,
	title = {CaO-P                    2O
                    5 Glass-Hydroxyapatite Thin Films Obtained by Laser Ablation: Characterisation and In Vitro Bioactivity Evaluation
},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Ferraz, M.P.; Inst. de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua Campo Alegre 823, 4150-180 Porto, Portugal; email: mpferraz@ufp.ptReferences: Arias, J.L., (1997) J Mater. Sci. Mater. Med., 8, pp. 873-876;Peraire, C., (1997) 13 th European Conference on Biomaterials, p. 75. , G{\"o}teborg, Sweden, 4-7 Sept; 
Ferraz, M.P., Monteiro, F.J., Santos, J.D., (1999) J Biomed Mater Res, 45, p. 373; 
Ferraz, M.P., Fernandes, M.H., Trigo-Cabral, A., Santos, J.D., Monteiro, F.J., (1999) J. Mat Sci: Mat. Med, 10 (9), p. 567
},
	month = {2004///},
	pages = {347 - 350},
	address = {Porto},
	abstract = {Hydroxyapatite (HA) coatings have been applied to improve adhesion of non-cemented implants to host bone. Plasma spraying is the most common technique leading to thick calcium phosphate films (\&gt;120μm). Pulse laser deposition (PLD), is a possible alternative method to obtain thin (\&lt;10 μm), well adherent hydroxyapatite (HA) films. Similarly to synthetic HA, biological apatites contain Ca                        2+, PO
                        4
                        3- and OH
},
	keywords = {2O, 5 glass, Adhesion, Bioactivity, Bone, Calcium Compounds, CaO-P, Coatings, Deposition, Energy dispersive spectroscopy, Homogeneous mixtures, Hydroxylapatite thin films, Implants (surgical), In vitro bioactivity, Laser ablation, Plasma spraying, Scanning electron microscopy, Thin films, X ray diffraction},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0347516195\&partnerID=40\&md5=ddd47b73956669a35aa9041ce9102612},
	author = {Ferraz, M.P. and Monteiro, F.J. and Gi{\~a}o, D. and Leon, B. and Gonzalez, P. and Liste, S. and Serra, J. and Arias, J. and Perez-Amor, M.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@book {2494,
	title = {Combining Foaming and Starch Consolidation Methods to Develop Macroporous Hydroxyapatite Implants},
	series = {Key Engineering MaterialsKey Eng Mat},
	volume = {254-256},
	year = {2004},
	note = {Conference code: 62053Cited By (since 1996): 10Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Lemos, A.F.; Depto. de Engenharia Cer{\^a}mica, CICEO, Campus de Santiago, 3810-193 Aveiro, Portugal; email: ilemos@cv.ua.ptReferences: De Groot, K., (1983) Bioceramics of Calcium Phosphates, , CRC Press, Boca Raton, FL;Hench, L.L., Wilson, J., (1993) An Introduction to Bioceramics, , World Scientific, London, UK; 
Ohgushi, H., Okumura, M., Yoshikawa, T., Inoue, K., Senpuku, N., Tamai, S., Shors, E.C., (1992) J. Biomed. Mater. Res., 26, p. 885; 
Minnear, W.P., (1992) Processing of Foamed Ceramics, Ceramic Transactions, Forming Science and Technology for Ceramics Vol. 26, 26. , ed. M.J. Cima, The American Ceramic Society, USA; 
Omatete, O., Janney, M.A., Strehlow, R.A., (1991) Am. Ceram Soc.Bull., 70, p. 1641; 
Kosmac, T., Novak, S., Sajko, M., (1995) Fourth Euro Ceramics, Vol. 1, Basic Science Developments, 1. , ed. C. Galassi. Gruppo Editoriale Faenza Editrice S.p.a., Italy; 
Graule, T.J., Baader, F.H., Gauckler, L.J., (1994) Cfi/Ber. DKH., 71, p. 317; 
Novich, B.E., Sundback, C.A., Adams, R.W., (1992) Ceramic Transactions, 26. , ed. M. J. Cima. American Ceramic Society, USA; 
Rives, R.D., U. S. Patent 4113480 (1976)Lycfeldt, O., Brandt, J., Lesca, S., (2000) J. Eur. Ceram. Soc., 20 (14-15), p. 2551; 
Olhero, S.M., Tar{\`\i}, G., Coimbra, M.A., Ferreira, J.M.F., (2000) J. Eur. Ceram. Soc., 20 (4), p. 423; 
Lycfeldt, O., Ferreira, J.M.F., (1998) J. Eur. Ceram. Soc., 18, p. 131; 
Lemos, A.F., Santos, J.D., Ferreira, J.M.F., (2003) Advanced Materials Forum, , in press
},
	month = {2004///},
	pages = {1041 - 1044},
	address = {Porto},
	abstract = {Hydroxyapatite (HA) is the calcium-phosphate material with composition closest to that of bone, what makes it suitable for osseous implant purposes. This material was used to produce macroporous structures with pores larger than 100 μm, which are believed to be suitable for allowing bone ingrowth. The macroporous structures were generated and consolidated by combining foaming and starch consolidation methods. The porous structures could be tailored according to the final application by varying the proportion of different foaming agents, foam-bath concentrate (FBC) and sodium lauril sulphate (SLS). Playing with these proportions it was also possible to improve foam stability and model the size of pores and pore interconnections in order to reproduce the pore structure of natural bone.},
	keywords = {Bioceramics, Biocompatibility, Bone, Composition, Foaming method, hydroxyapatite, Hydroxyapatlte, Implants (surgical), Macroporous structures, Microstructure, Pore size, Porous ceramics, Porous materials, Starch, Starch consolidation},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0346885485\&partnerID=40\&md5=63e5b33a0a2185af44b629c565e5240f},
	author = {Lemos, A.F. and Ferreira, J.M.F.},
	editor = {Barbosa M.A. and Monteiro F.J. and Correia R. and Leon B.}
}
@article {2471,
	title = {A comparative study of CaO-P                    2O
                    5-SiO
                    2 gels prepared by a sol-gel method
},
	journal = {Materials Chemistry and PhysicsMater Chem Phys},
	volume = {88},
	year = {2004},
	note = {Cited By (since 1996): 9Export Date: 21 November 2012Source: ScopusCODEN: MCHPDdoi: 10.1016/j.matchemphys.2004.06.015Language of Original Document: EnglishCorrespondence Address: Hussain, N.S.; DEMM, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal; email: sooraj@fe.up.ptReferences: Hench, L.L., (1991) J. Am. Ceram. Soc., 74, p. 1487;Hench, L.L., Wilson, J., (1993) An Introduction to Bioceramics, , World Scientific, Singapore; 
Ohtsuki, C., Kokubo, T., Yamamuro, T., (1992) J. Non-cryst. Solids, 143, p. 84; 
Kokubo, T., Yoshihara, S., Nishimura, N., Yamamuro, T., Nakamura, T., (1991) J. Am. Ceram. Soc., 74, p. 1739; 
Ota, Y., Iwashita, T., Kasuga, T., Abe, Y., Seki, A., (2001) Key Eng. Mater., 192-195, p. 421; 
Kasuga, T., Ota, Y., Nogami, M., Abe, Y., (1996) Phos. Res. Bull., 6, p. 75; 
Ota, Y., Kasuga, T., Abe, Y., (1997) J. Am. Ceram. Soc., 80 (1), p. 225; 
Zhang, Y., Santos, J.D., (2000) J. Non-cryst. Solids, 227, p. 14; 
Yoldas, B.E., (1975) Ceram. Bull., 54, p. 286; 
Thomas, I.M., (1988) Sol-gel Technology for Thin Films, Fibres, Preforms, Electronics and Specialty Shapes, p. 2. , L.C. Klein (Ed.), Noyes, NJ; 
Brinker, C.J., Scherer, G.W., (1990) Sol-gel Science: The Physics and Chemistry of Sol-gel Processing, , Academic Press, New York; 
Hench, L.L., West, J.K., (1990) Chem. Rev., 90, p. 33; 
Hench, L.L., (1998) Sol-gel Silica: Properties, Processing and Technology Transfer, , Noyes, New Jersey; 
Salinas, A.J., Martin, A.I., Vallet-Reg{\'\i}, M., (2002) J. Biomed. Mater. Res., 61 (4), p. 524; 
Laczka, M., Cholewa, K., Lazca-Osyczka, A., (1997) J. Alloys Comp., 248, p. 42; 
Roman, J., Padilla, S., Vallet-Reg{\'\i}, M., (2003) Chem. Mater., 15 (3), p. 798; 
Li, R., Clark, A.E., Hench, L.L., (1991) J. Appl. Biomater., 2, p. 231; 
R{\'a}mila, A., Vallet-Reg{\'\i}, M., (2001) Biomaterials, 22, p. 2301; 
Balas, F., Arcos, D., P{\'e}rez-Pariente, J., Vallet-Reg{\'\i}, M., (2001) J. Mater. Res., 16 (5), p. 1345; 
Peitl, O., Zanotto, E.D., Hench, L.L., (2001) J. Non-cryst. Solids, 292, p. 115; 
Casu, M., Casula, M.F., Corrias, A., Pachina, G., (2003) J. Non-cryst. Solids, 315, p. 97; 
Motke, S.G., Yawale, S.P., Yawale, S.S., (2002) Bull. Mater. Sci., 25 (1), p. 75; 
M{\'e}ndez-Vivar, J., Mendoza-Bandala, A., (2000) J. Non-cryst. Solids, 261, p. 127; 
Popa, M., Calderon-Moreno, J.M., Popescu, L., Kakihana, M., Torecillas, R., (2002) J. Non-cryst. Solids, 297, p. 290; 
Sooraj Hussain, N., Prabhakara Reddy, Y., Buddhudu, S., (2002) Spectrosc. Lett., 35 (2), p. 275; 
Mackenzie, J.D., (1986) Science of Ceramic Chemical Processing, p. 112. , L.L. Hench, D.R. Ulrich (Eds.), Wiley/Interscience; 
Aoki, H., (1991) Science and Medical Applications of Hydroxyapatite, , Jpn. Assoc. Apatite Sci. (JASS)
},
	month = {2004///},
	pages = {5 - 8},
	abstract = {This paper reports on the results concerning the preparation and systematic analysis of CaO-P                        2O
                        5-SiO
                        2 gels by a sol-gel process. The concentration of SiO
                        2 influence on these gels and found the results are very encouraging. Studies have revealed that the concentration of SiO
                        2 has a marginal influence on the phase formation of these gels. It has appreciable changes from less configured particles at lower values of concentration, with an ultimate growth in a highly crystalline fibrous nature at higher concentrations of SiO
                        2. The authors have examined the changes in the morphology of these gels by a scanning electron microscope with an energy dispersive analyser (SEM/EDS techniques) and also from the profiles of Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) spectral data and reported the crystalline product containing the fibrous β-Ca(PO
                        3)
                        2 as a major phase in the materials studied. {\textcopyright} 2004 Elsevier B.V. All rights reserved.
},
	keywords = {Bioactivity, Calcium Compounds, Calcium phosphosilicates, Fourier transform infrared spectroscopy, Gels, morphology, Phase composition, Phase formation, Phosphorus Compounds, Scanning electron microscopy, Silica, Sol-gel process, Sol-gels, X ray diffraction analysis},
	isbn = {02540584 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-4644336194\&partnerID=40\&md5=14333a8b36bde426323bc7e90ad3386e},
	author = {Hussain, N.S. and Lopes, M.A. and Santos, J.D.}
}
@article {2483,
	title = {Crystallisation studies of biodegradable CaO-P                    2O
                    5 glass with MgO and TiO
                    2 for bone regeneration applications
},
	journal = {Glass TechnologyGlass Technol},
	volume = {45},
	year = {2004},
	note = {Cited By (since 1996): 5Export Date: 21 November 2012Source: ScopusCODEN: GLSTALanguage of Original Document: EnglishCorrespondence Address: Santos, J.D.; INEB, Inst. de Engenharia Biom{\'e}dica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: jdsantos@fe.up.ptReferences: Siligardi, C., D{\textquoteright}Arrigo, M.C., Leonelli, C., Sintering behavior of glass ceramics frits (2000) Am. Ceram. Soc. Bull., pp. 88-92;Clarck, T.J., Reed, J.S., Kinetic processes involved in the sintering and crystallization of glass powders (1986) J. Am. Ceram. Soc., 69 (11), pp. 837-846; 
Karmakar, B., Kundu, P., Jana, S., Dwivedi, R.N., Crystallization kinetics and mechanism of low-expansion lithium aluminosilicate glass-ceramics by dilatometry (2002) J. Am. Ceram. Soc., 85 (10), pp. 2572-2577; 
Zhang, Y., Santos, J.D., Crystallization and microstructure analysis of calcium phosphate-based glass ceramics for biomedical applications (2000) J. Non-cryst. Solids, 272 (1), pp. 14-21; 
Ryu, H.-S., Youn, H.-J., Chang, B.-S., Lee, C.-K., Chung, S.-S., An improvement in sintering property of β-tricalcium phosphate by addition of calcium pyrophosphate (2002) Biomaterials, 23, pp. 909-914; 
Kitsugi, T., Yamamuro, T., Nakamura, T., Ota, M., Transmission electron microscopy observations at the interface of bone and four types of calcium phosphate ceramics with different calcium/phosphorous molar ratios (1995) Biomaterials, 16, pp. 1101-1107
},
	month = {2004///},
	pages = {78 - 79},
	abstract = {CaO-P                        2O
                        5 glass ceramics with small additions of MgO and TiO
                        2 were prepared in the ortho and pyrophosphate regions by powder sintering and crystallisation through heat treatment at different temperatures determined from differential thermal analysis (DTA). The glass ceramics were analysed by x-ray diffraction (XRD) and it was observed that α- and β-Ca
                        2P
                        2O
                        7 were the first phases to precipitate in the parent glass matrix at a temperature of 681{\textdegree}C, followed by CaTi
                        4(PO
                        4)
                        6 and TiP 
                        2O
                        7 at temperatures between 703 and 725{\textdegree}C. The β-Ca
                        2P
                        2O
                        7 and CaTi
                        4(PO 
                        4)
                        6 phases are usually considered as biocompatible and bioactive and, therefore, these glass ceramics seem to show good potential for bone regeneration applications.
},
	keywords = {Biodegradation, Bone Regeneration, Crystallization, Differential thermal analysis, Diffractometers, Glass ceramics, Glass matrix, Glass transition, Laser diffraction, Lime, Magnesia, methanol, Monochromators, Precipitation (chemical), Temperature scanning, Titanium dioxide, X ray powder diffraction},
	isbn = {00171050 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-3042549927\&partnerID=40\&md5=62f3cbbb74e944ac2e066db7432db201},
	author = {Dias, A.G. and Tsuru, K. and Hayakawa, S. and Lopes, M.A. and Santos, J.D. and Osaka, A.}
}
@article {2511,
	title = {Cartographic representation of spatial data},
	journal = {Representa{\c c}{\~a}o Cartogr{\'a}fica de Dados EspaciaisArquivos de MedicinaArq. Med.},
	volume = {17},
	year = {2003},
	note = {Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusCODEN: ARQMELanguage of Original Document: PortugueseCorrespondence Address: De Pina, M.D.F.; INEB, Instituto de Engenharia Biomedica, Laboratorio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: fpina@ibmc.up.ptReferences: Tyner, J., (1992) Introduction to Thematic Cartography, , Prentice Hall;Dent, B., (1996) Cartography, Thematic Map Design, , WCB Publishers; 
Monmonier, M., (1993) Mapping It Out: Expository Cartography for the Humanities and Social Sciences, , The University of Chicago Press; 
Kraak, M.J., Ormeling, F.J., (1996) Cartography: Visualization of Spatial Data, , Longman; 
Burrough, P.A., McDonnell, R., (1998) Principles of Geographical Information Systems, , Oxford University Press
},
	month = {2003///},
	pages = {233 - 240},
	keywords = {article, cartography, diagram, dot map, epidemiological data, geographic distribution, histogram, population distribution, tuberculosis, visual aid},
	isbn = {08713413 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-1642488242\&partnerID=40\&md5=621375e84838782b2de7b7a67e26581d},
	author = {De Pina, M.D.F.}
}
@article {2508,
	title = {Constructing thromboresistant surface on biomedical stainless steel via layer-by-layer deposition anticoagulant},
	journal = {BiomaterialsBiomaterials},
	volume = {24},
	year = {2003},
	note = {Cited By (since 1996): 67Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(03)00363-6PubMed ID: 12951013Language of Original Document: EnglishCorrespondence Address: Ji, J.; Department of Polymer Science, Zhejiang University, Hangzhou 310027, China; email: jianji@mail.hz.zj.cnChemicals/CAS: heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; polyethyleneimine, 74913-72-7; stainless steel, 12597-68-1; trometamol, 1185-53-1, 77-86-1; Anticoagulants; Coated Materials, Biocompatible; Heparin, 9005-49-6; Polyethyleneimine, 9002-98-6; Stainless Steel, 12597-68-1Manufacturers: AldrichReferences: Bertrand, P., Jonas, A., Laschewsky, A., Legras, R., Ultrathin polymer coating by complexation of polyelectrolytes at interfaces: Suitable materials, structure and properties (2000) Macromol Rapid Commun, 21, pp. 319-348;Ulman, A., Formation and structure of self-assembled monolayers (1996) Chem Rev, 96, pp. 1533-1554; 
Sano, M., Lvov, Y., Kunitake, T., (1996) Annu Rev Mater Sci, 26, pp. 153-187; 
Laschewsky, A., (1997) Eur Chem Chron ECC, 2, pp. 13-24; 
Picart, C., Lavalle, Ph., Hubert, P., Cuisnier, Fj., Decher, G., Schaaf, P., Voegel, J.C., Buildup mechanism for poly (L-lysine)/hyaluronic acid films onto a solid surface (2001) Langmuir, 17, pp. 7414-7424; 
Houska, M., Brynda, E., Interactions of proteins with polyelectrolytes at solid/liquid interfaces: Sequential adsorption of albumin and heparin (1997) J Colloid Interface Sci, 188, pp. 243-250; 
Brynda, E., Houska, M., Multiple alternating molecular layers of albumin and heparin on solid surfaces (1996) J Colloid Interface Sci, 183, pp. 18-25; 
Thomann, U.I., Uggowitzer, P.J., Wear-corrosion behavior of biocompatible austenitic stainless steels (2000) Wear, 239, pp. 48-58; 
Cheatham, J., Improved stents for pediatric applications (2001) Prog Pediatr Cardiol, 14, pp. 95-115; 
Donachie, M., Biomedical alloys (1998) Adv Mater Process, 154, pp. 63-65; 
Decher, G., Fuzzy nanoassemblies: Toward layered polymeric multicomposites (1997) Science, 277, pp. 1232-1237; 
Raposo, M., Pontes, R.S., Mattoso, L.H.C., Oliveira, O.N., Kinetics of adsorption of poly (o-methoxyaniline) self-assembled films (1997) Macromolecules, 30, pp. 6095-6101; 
Vov, Y.L., Rusling, J., Thomsen, D.L., Papadimitrakopoulos, F., Kawakami, T., Kunitake, T., High-speed multilayer film assembly by alternate adsorption of silica nanoparticles and linear polycation (1998) Chem Commun, 11, pp. 1229-1230; 
Lowack, K., Helm, C.A., Controlling the self-assembly process of polyelectrolyte multilayers (1998) Macromolecules, 31, pp. 823-833; 
Cheng, Z., Cheng, L., Dong, S., Yang, X., Formation and characterization of heteropolyacid/polycation multilayer films on gold electrode (2001) J Electrochem Soc, 148, p. 227
},
	month = {2003///},
	pages = {4699 - 4705},
	abstract = {Multilayer films consisting of polyethylenimine (PEI) and heparin were successfully prepared on biomedical 316L stainless steel surface via electrostatic self-assembly (ESA) of the PEI and heparin. The process of ESA of PEI/heparin was monitored by static contact angle, electrochemical impedance spectroscopy (EIS), reflection adsorption spectroscopy and X-ray photoelectron spectroscopy data. The contact angle and EIS data revealed that the multilayer coating was stable in Tris-HCl (pH 7.35) buffer solution for 21 days. The static platelet adhesion and static clotting time experiments indicated that the PEI/heparin-deposited stainless steel could resist the platelet adhesion and prolong the static clotting time effectively. Such an easy processing and shape-independent method may have good potential for surface modification of cardiovascular devices. {\textcopyright} 2003 Elsevier Ltd. All rights reserved.},
	keywords = {316L stainless steel, Adhesion, Adsorption, anticoagulant agent, Anticoagulants, aqueous solution, article, Biocompatibility, biomaterial, Biomaterials, blood clotting time, Blood Platelets, Blood-compatibility, buffer, Coated Materials, Biocompatible, coating, Coatings, Contact angle, controlled study, Deposition, device, electricity, Electrochemical impedance spectroscopy, electrochemistry, Electrostatics, heparin, human, human cell, Humans, implant, Layer-by-layer, material coating, materials testing, Multilayers, Organic polymers, pH, Platelet Adhesiveness, Polyethyleneimine, priority journal, reflection adsorption spectroscopy, Self assembly, Spectroscopy, Stainless steel, Surface Properties, surface property, Surfaces, thrombocyte adhesion, Thromboresistant surfaces, trometamol, X ray photoelectron spectroscopy},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0041386468\&partnerID=40\&md5=a68c77a7525bca1760cc298a394c713f},
	author = {Tan, Q. and Ji, J. and Barbosa, M.A. and Fonseca, C. and Shen, J.}
}
@article {2521,
	title = {Corrosion behaviour of commercially pure titanium shot blasted with different materials and sizes of shot particles for dental implant applications},
	journal = {BiomaterialsBiomaterials},
	volume = {24},
	year = {2003},
	note = {Cited By (since 1996): 77Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(02)00314-9Language of Original Document: EnglishCorrespondence Address: Aparicio, C.; Res. Center in Biomed. Eng. (CREB), Department of Materials Science, Universitat Politecnica de Catalunya, Av. Diagonal 647, 08028 Barcelona, Spain; email: conrado.aparicio@upc.esChemicals/CAS: Dental Implants; Titanium, 7440-32-6References: Br{\r a}nemark, P.I., Hansson, B.O., Adell, R., Breine, U., Lindstr{\"o}m, J., Hallen, O., {\"O}hman, A., Osseointegrated implants in the treatment of edentulous jaw experience from a 10-year period (1977) Scand J Reconstr Surg, 16 (SUPPL.), pp. 1-132;Lemons, J.E., Application of materials in medicine and dentistry. Dental implants (1996) Biomaterials Science: An Introduction to Materials in Medicine, pp. 308-318. , B.D. Ratner, A.S. Hoffman, F.J. Schoen, \& J.E. Lemons. San Diego: Academic Press; 
Pourbaix, M., Electrochemical corrosion of metallic biomaterials (1984) Biomaterials, 5, pp. 122-134; 
Bardos, D.I., Titanium and titanium alloys (1990) Encyclopaedia of Medical and Dental Materials, pp. 360-365. , D. William. Oxford: Pergamon Press; 
Boyer, R., Welsh, G., Colling, E.W., (1994) Materials Properties Handbook: Titanium Alloys, pp. 181-219. , Ohio: ASM International; 
Kasemo, B., Lausmaa, J., Surface science aspects on inorganic biomaterials (1986) CRC Crit Rev Biocompatibilty, 4, pp. 335-380; 
Tengvall, P., Lundtr{\"o}m, I., Physico-chemical considerations of titanium as a biomaterial (1992) Biomaterials, 9, pp. 115-134; 
Albrektsson, T., Br{\r a}nemark, P.I., Hansson, B.O., Lindstr{\"o}m, J., Osseointegrated titanium implants (1981) Acta Orthop Scand, 52, pp. 155-170; 
Thomas, K.A., Cook, S., An evaluation of variables influencing implant fixation by direct bone apposition (1985) J Biomed Mater Res, 19, pp. 875-901; 
Wennerberg, A., (1996) On Surface Roughness and Implant Incorporation, , Ph.D. thesis, G{\"o}teborg: University of G{\"o}teborg; 
Bowers, K.T., Keller, J.C., Randolph, B.A., Wick, D.G., Michaels, C.M., Optimization of surface micromorphology from enhanced osteoblast response in vitro (1992) Int J Oral Maxillofac Implants, 7, pp. 302-310; 
Groessner-Schereider, B., Tuan, R.S., Enhanced extracellular matrix production and mineralization by osteoblasts cultured on titanium surfaces in vitro (1992) J Cell Sci, 101, pp. 209-217; 
Schwartz, Z., Boyan, B.D., Underlaying mechanisms at the bone biomaterial interface (1994) J Cell Biochem, 56, pp. 340-347; 
Boyan, B.D., Hummert, T.W., Kieswetter, K., Schraub, D., Dean, D.D., Schwartz, Z., Effect of titanium surface characteristics on chondrocytes and osteoblasts in vitro (1995) Scanning Electron Microsc (Cells and Materials), 5, pp. 323-335; 
Martin, J.Y., Schwartz, Z., Hummert, T.W., Schraub, D.M., Simpson, J., Lankford, J., Dean, D.D., Boyan, B.D., Effect of titanium surface roughness on proliferation, differentiation and protein synthesis of human osteoblast-like cells (MG63) (1995) J Biomed Mater Res, 29, pp. 389-401; 
Ong, J.L., Prince, C.W., Raikar, G.N., Lucas, L.C., Effect of surface topography of titanium on surface chemistry and cellular response (1996) Implant Dent, 5, pp. 83-88; 
Deligianni, D.D., Katsal, N., Ladas, S., Sotiropoulou, D., Amedee, J., Missirlis, Y.F., Effect of surface roughness of the titanium alloy Ti-6Al-4V on human bone marrow cell response and on protein adsorption (2001) Biomaterials, 22, pp. 1241-1251; 
Schwartz, Z., Lohmann, C.H., Sisk, M., Cochran, D.L., Sylvia, V.L., Simpson, J., Dean, D.D., Boyan, B.D., Local factor production by MG63 ostoblast-like cells in response to surface roughness and 1,25-(OH) 2D 3 is mediated via protein kinase C- and protein kinase A-dependent pathways (2001) Biomaterials, 22, pp. 731-741; 
Predecki, P., Stephan, J.E., Auslander, B.A., Mooney, V.L., Kirkland, K., Kinetics of bone growth into cylindrical channels in aluminium oxide and titanium (1972) J Biomed Mater Res, 6, pp. 375-400; 
Buser, D., Schenk, R.K., Steinemann, S., Fiorelini, J.P., Fox, C.H., Stich, H., Influence of surface characteristics on bone integration of titanium implants. A histomorphometric study in miniature pigs (1991) J Biomed Mater Res, 25, pp. 889-902; 
Ericsson, I., Johansson, C., Bystedt, H., Norton, M.R., A histomorphometric evaluation of bone-to-implant contact on machined-prepared roughened titanium dental implants (1994) Clin Oral Implant Res, 5, pp. 202-206; 
Feighan, J.E., Goldberg, V.M., Davy, D., Parr, J.A., Stevenson, S., The influence of surface-blasting on the incorporation of titanium alloy implants in a rabit intramedullary model (1995) J Bone Jt Surg Am, 77, pp. 1380-1395; 
Wennerberg, A., Albrektsson, T., Andersson, B., An animal study of c.p. titanium screws with different surface topographies (1995) J Mater Sci: Mater Med, 6, pp. 302-309; 
Wong, M., Eulenberger, J., Schenk, R., Hunziker, E., Effect of surface topology on the osseointegration of implant materials in trabecular bone (1995) J Biomed Mater Res, 29, pp. 1567-1575; 
Wennerberg, A., Albrektsson, T., Johansson, C., Andersson, B., Experimental study of turned and grit-blasted screw-shaped implants with special emphasis on effects of blasting material and surface topography (1996) Biomaterials, 17, pp. 15-22; 
Cochran, D.L., Schenk, R.K., Lussi, A., Higginbottom, F.L., Buser, D., Bone response to unloaded and loaded titanium implants with a sandblasted and acid etched surface: A histometric study in the canine mandible (1998) J Biomed Mater Res, 40, pp. 1-11; 
Piatelli, A., Manzon, L., Scarano, A., Paolantonio, M., Piatelli, M., Histologic and histomorphometric analysis of the bone response to machined and sandblasted titanium implants: An experimental study in rabbits (1998) Int J Oral Maxillofac Implants, 13, pp. 805-810; 
Svehla, M., Morberg, P., Zicat, B., Bruce, W., Sonnabend, D., Walsh, W.R., Morphometric and mechanical evaluation of titanium implant integration: Comparison of five surface structures (2000) J Biomed Mater Res, 51, pp. 15-22; 
Gotfredsen, K., Nimb, L., Hjorting-Hansen, E., Jensen, J.S., Holmen, A., Histomorphometric and removal torque analysis for TiO 2-blasted titanium implants (1994) Clin Oral Implant Res, 5, pp. 202-206; 
Jones, F., Teeth and bones: Applications of surface science to dental materials and related biomaterials (2001) Surf Sci Rep, 42, pp. 75-205; 
Naji, A., Harnad, M.F., Cytocompatibility of two coating materials, amorphous alumina and silicon carbide, using human differentiated cell cultures (1991) Biomaterials, 12, pp. 690-694; 
Darvell, B.W., Samman, N., Luk, W.K., Clark, K.F., Tideman, H., Contamination of titanium casting by aluminium oxide blasting (1995) J Dent, 23, pp. 319-322; 
Placko, H.E., Mishra, S., Weimer, J.J., Lucas, L.C., Surface Characterization of titanium-based implant materials (2000) Int J Oral Maxillofac Implants, 15, pp. 355-363; 
Orsini, G., Assenza, B., Scarano, A., Piatelli, M., Piatelli, A., Surface analysis of machined versus sandblasted and acid-etched titanium implants (2000) Int J Oral Maxillofac Implants, 15, pp. 779-784; 
Gross, U., Strunz, V., The interface of various glasses and glass ceramics with a bony implantation bed (1985) J Biomed Mater Res, 19, pp. 251-271; 
Aparicio, C., Gil, F.J., Peraire, C., Padr{\'o}s, A., Planell, J.A., Effect of size and material of the shot peening particles on adhesion and differentiation of osteoblast-like cell cultures on commercially pure titanium (1998) North Sea Biomaterials 1998 Proceedings, p. 137. , The Hague; 
Fontana, M.G., Green, N.D., (1978) Corrosion Engineering: Mc Graw Hill Series in Materials Science and Engineering, , USA: McGraw-Hill; 
Standard practice for laboratory immersion corrosion testing metals (1992) 1992 Annual Book of ASTM Standards, pp. 102-109. , vol. 03.02. Philadelphia: ASTM; 
Boukamp, B.A., (1989) Equivalent Circuit (EQUIVCRTPAS). User{\textquoteright}s Manual, , Enschede: University of Twente; 
Standard test method for conducting cyclic potentiodynamic polarization measurements for localized corrosion susceptibility of iron-nickel, or cobalt-based alloys (1992) 1992 Annual Book of ASTM Standards, pp. 231-235. , vol. 03.02. Philadelphia: ASTM; 
(1984) Norme FEPA des Grains de Corindon Electrofondu et de Carbure de Silicium Pour Abrasifs Agglomeres, , Paris: Fed{\'e}ration Europ{\'e}enne des Fabricants de Produits Abrasifs; 
Bohn, P., Qualitaty control of shot media by sieve analysis (1999) Shot Peener, 13, pp. 6-9; 
Mehelich, C.S., Van Kuiken, L., Woelfel, M.M., Shot peening (1995) ASM Metals Handbook. Vol. 5: Surface Engineering, 5, pp. 138-149. , USA: ASM International; 
Udupa, G., Singaperumal, M., Sirohi, R.S., Kothiyal, M.P., Characterization of surface topography by confocal microscopy: II. The micro and macro surface irregularities (2000) Meas Sci Technol, 11, pp. 315-329; 
Arnell, R.D., Davie, P.B., Halling, J., Whomes, T.L., (1991) Tribology: Principles and Design Applications, , London: Mac Millan; 
Udupa, G., Singaperumal, M., Sirohi, R.S., Kothiyal, M.P., Characterization of surface topography by confocal microscopy: I. Principles and the measurement system (2000) Meas Sci Technol, 11, pp. 305-314; 
Solar, R.J., Pollack, S.R., Korostoff, E., In vitro corrosion testing of titanium surgical implant alloys: An approach to understanding titanium release from implants (1979) J Biomed Mater Res, 13, pp. 217-250; 
Hoar, T.P., Mears, D.C., Corrosion resistant alloys in chloride solutions, materials for surgical implants (1966) Proc Royal Soc London Ser A, 294, pp. 486-510; 
Gonzalez, J.E.G., Mirza-Rosca, J.C., Study of the corrosion behaviour of titanium and some of its alloys for biomedical and dental implant applications (1999) J Electroanal Chem, 471, pp. 109-115; 
McDonald, J.R., (1987) Impedance Spectroscopy, , New York: Wiley; 
Aziz-Kerrzo, M., Conroy, K.G., Fenelson, A.M., Farrell, S.T., Breslin, C.B., Electrochemical studies on the stability and corrosion resistance of titanium-based implant materials (2001) Biomaterials, 22, pp. 1531-1539; 
Cai, Z., Nakajima, H., Woldu, M., Berglund, A., Bergman, M., Okabe, T., In vitro corrosion resistance of titanium made using different fabrication methods (1999) Biomaterials, 20, pp. 183-190; 
Kobayashi, M., Matsui, T., Murakami, Y., Mechanism of creation of compressive residual stresses by shot peening (1998) Int J Fatigue, 20, pp. 351-357; 
Prevey, P.S., X-ray diffraction characterization of residual stresses produced by shot peening (2001) Shot Peener, 15, pp. 4-8; 
Mueller, H.J., Greener, E.H., Polarization studies of surgical materials in Ringer{\textquoteright}s solution (1970) J Biomed Mater Res, 4, pp. 29-41; 
Speck, K.M., Fraker, A.C., Anodic polarization behavior of Ti-Ni and Ti-6Al-4V in simulated physiological solution (1980) J Dent Res, 59, pp. 1590-1595; 
Sousa, S.R., Barbosa, M.A., Corrosion resistance of titanium cp in saline physiological solutions with calcium phosphate and proteins (1993) Biomaterials, 14, pp. 287-294; 
Khan, M.A., Williams, R.L., Williams, D.F., In-vitro corrosion and wear of titanium alloys in the biological environment (1996) Biomaterials, 17, pp. 2117-2126; 
Ewers, G.J., Greener, E.H., The electrochemical activity of the oral cavity - A new approach (1985) J Oral Rehab, 12, pp. 469-476
},
	month = {2003///},
	pages = {263 - 273},
	abstract = {It is well known that the osseointegration of the commercially pure titanium (c.p. Ti) dental implant is improved when the metal is shot blasted in order to increase its surface roughness. This roughness is colonised by bone, which improves implant fixation. However, shot blasting also changes the chemical composition of the implant surface because some shot particles remain adhered on the metal. The c.p. Ti surfaces shot blasted with different materials and sizes of shot particles were tested in order to determine their topographical features (surface roughness, real surface area and the percentage of surface covered by the adhered shot particles) and electrochemical behaviour (open circuit potential, electrochemical impedance spectroscopy and cyclic polarisation). The results demonstrate that the increased surface area of the material because of the increasing surface roughness is not the only cause for differences found in the electrochemical behaviour and corrosion resistance of the blasted c.p. Ti. Among other possible causes, those differences may be attributed to the compressive residual surface stresses induced by shot blasting. All the materials tested have an adequate corrosion and electrochemical behaviour in terms of its possible use as dental implant material. {\textcopyright} 2002 Elsevier Science Ltd. All rights reserved.},
	keywords = {article, Blast finishing, chemical composition, chemistry, Corrosion, Dental implant, Dental Implants, Dental prostheses, Electric Potential, Electrochemical impedance spectroscopy, electrochemistry, impedance, materials testing, Particle Size, Polarization, priority journal, Shot blasting, Spectroscopy, stress, Surface roughness, Surface topography, Titanium, tooth implantation},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037209960\&partnerID=40\&md5=c60c6b69f4fb345454b557acc836ec95},
	author = {Aparicio, C. and Javier Gil, F. and Fonseca, C. and Barbosa, M. and Planell, J.A.}
}
@article {2506,
	title = {Creating maps: The construction of a lecture on the specialties of phenomena},
	journal = {Elaborar Mapas: A Constru{\c c}{\~a}o de um Discurso sobre a Espacialidade dos Fen{\'o}menosArquivos de MedicinaArq. Med.},
	volume = {17},
	year = {2003},
	note = {Export Date: 21 November 2012Source: ScopusCODEN: ARQMELanguage of Original Document: PortugueseCorrespondence Address: Henrique Castiglione, L.; Depto. de Engenharia Cartografica, Univ. do Estado do Rio de Janeiro, Rua S{\~a}o Francisco Xavier, 524, Rio de Janeiro, Brazil; email: lhcasti@vetor.com.brReferences: Joly, F., (1997) A Cartografia, , Editora Papirus;MacEachren, A., (1995) How Maps Work: Representation, Visualization and Design, , The Guilford Press; 
Barcellos, C., Santos, S., (1997) Colocando Dados no Mapa: A Escolha da Unidade Especial de Agrega{\c c}{\~a}o e Integra{\c c}{\~a}o de Bases de Dados em Sa{\'u}de e Ambiente Atrav{\'e}s do Geoprocessamento, 6, pp. 21-29. , Informe epidemiol{\'o}gico do Sistema {\'U}nico de Sa{\'u}de, Brasil; 
Bertin, J., (1977) La Graphique et le Traitement Graphique de L{\textquoteright}information, , Flamarion; 
Dent, B., (1996) Cartography: Thematic Map Design, , WCB Publishers; 
Tyner, J., (1992) Introduction to Thematic Cartography, , Prentice Hall; 
Kraak, M., Ormeling, F., (1996) Cartography: Visualization of Spatial Data, , Longman; 
Monmonier, M., (1993) Mapping It Out: Expository Cartography for the Humanities and Social Sciences, , The University of Chicago Press; 
Dorling, D., Fairbaim, D., (1997) Mapping: Ways of Representing the World, , Longman
},
	month = {2003///},
	pages = {303 - 310},
	keywords = {audiovisual equipment, geography, information processing, interpersonal communication, medical education, medical specialist, review},
	isbn = {08713413 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-1842533211\&partnerID=40\&md5=c436eea1ef300362433b5c9d81c614af},
	author = {Henrique Castiglione, L. and De F{\'a}tima De Pina, M.}
}
@article {2533,
	title = {Cellulose phosphates as biomaterials. In vivo biocompatibility studies},
	journal = {BiomaterialsBiomaterials},
	volume = {23},
	year = {2002},
	note = {Cited By (since 1996): 45Export Date: 21 November 2012Source: ScopusCODEN: BIMADdoi: 10.1016/S0142-9612(01)00152-1PubMed ID: 11791931Language of Original Document: EnglishCorrespondence Address: Granja, P.L.; Laborat{\'o}rio de Biomateriais, INEB-Inst. de Engenliaria Biomedica, Rua do Campo Alegre, 823-4150-180 Porto, Portugal; email: pgranja@ibmc.up.ptChemicals/CAS: Biocompatible Materials; Calcium Radioisotopes; Calcium, 7440-70-2; Cellulose, 9004-34-6; phosphocellulose, 9015-14-9References: Jochanan, H., Boss, M.D., Osseointegration (1999) J Long-Term Eff Med Impl, 9, pp. 1-10;Albrektsson, T., Hard tissue response (1998) Handbook of biomaterial properties, pp. 500-512. , Black J, Hastings G, editors. London, UK: Chapman \& Hall; 
Hench, L.L., Bioactive materials: The potential for tissue regeneration (1998) J Biomed Mater Res, 41, pp. 511-517; 
Yaszemski, M.J., Payne, R.G., Hayes, W.C., Langer, R., Mikos, A.G., Evolution of bone transplantation: Molecular, cellular and tissue strategies to engineer human bone (1996) Biomaterials, 17, pp. 175-185; 
Hollinger, J., McAllister, B., Bone and its repair (1995) Bioceramics, 8, pp. 3-9. , Wilson J, Hench LL, Greenspon D, editors. FL, USA: Pergamon Press; 
Friedman, R.J., Black, J., Galante, J.O., Jacobs, J.J., Skinner, H.B., Current concepts in orthopaedic biomaterials and implant fixation (1993) J Bone Jt Surg, 75 A, pp. 1086-1109; 
Bakker, D., Grote, J.J., Vrouenraets, C.M.F., Hesseling, S.C., De Wijn, J.R., Van Blitterswijk, C.A., Bone-bonding polymer (polyactive{\texttrademark}) (1990) Clinical implant materials, Advances in Biomaterials Series, 9, pp. 99-104. , Heimke G, Solt{\'e}sz U, Lee AJC, editors. Amsterdam, The Netherlands: Elsevier; 
LeGeros, R.Z., Calcium phosphate materials in restorative dentistry: A review (1988) Adv Dent Res, 2, pp. 164-183; 
Hastings, G.W., Mahmud, F.A., Intelligent orthopaedic materials (1993) J Intell Mat Syst Str, 4, pp. 452-456; 
Black, J., (1988) Orthopaedic biomaterials in research and practice, , New York, USA: Churchill Livingstone; 
Li, P., Ye, X., Kangasniemi, I., De Blieck-Hogervorst, J.M.A., Klein, C.P.A.T., De Groot, K., In vivo calcium phosphate formation induced by sol-gel-prepared silica (1995) J Biomed Mater Res, 29, pp. 325-328; 
Tanahashi, M., Yao, T., Kokubo, T., Minoda, M., Miyamoto, T., Nakamura, T., Yamamuro, T., Apatite coated on organic polymers by biomimetic process: Improvement in its adhesion to substrate by glow-discharge treatment (1995) J Biomed Mater Res, 29, pp. 349-357; 
Li, P., Ohtsuki, T., Kokubo, T., Nakanishi, K., Soga, N., Nakamura, T., Yamamuro, T., Process of formation of bone-like apatite layer on silica gel (1993) J Mater Sci: Mater Med, 4, pp. 127-131; 
Radin, S.R., Ducheyne, P., The effect of calcium phosphate ceramic composition and structure on in vitro behavior. II. Precipitation (1993) J Biomed Mater Res, 27, pp. 35-45; 
Puleo, D.A., Nanci, A., Understanding and controlling the bone-implant interface (1999) Biomaterials, 20, pp. 2311-2321; 
McFarland, C.D., Mayer, S., Scotchford, C., Dalton, B.A., Steele, J.G., Downes, S., Attachment of cultured human bone cells to novel polymers (1999) J Biomed Mater Res, 44, pp. 1-11; 
Dalton, B.A., McFarland, C.D., Gengenbach, T.R., Griesser, H.J., Steele, J.G., Polymer surface chemistry and bone cell migration (1998) J Biomater Sci Polym Ed, 9, pp. 781-799; 
Scotchford, C.A., Cooper, E., Leggett, G.J., Downes, S., Growth of human osteoblast-like cells on alkenethiol on gold self-assembled monolayers: The effect of surface chemistry (1998) J Biomed Mater Res, 41, pp. 431-442; 
Adams, A.M., Soames, J.V., Searle, R.F., Ultrastructural changes in cultured human periodontal ligaments cells exposed to dental materials (1995) J Biomed Mater Res, 29, pp. 999-1004; 
Lamba, N.M.K., Baumgartner, J.A., Cooper, S.L., Cell-synthetic surface interactions (1998) Frontiers in tissue engineering, pp. 121-137. , Patrick CW, Mikos AG, McIntire LV, editors. Amsterdam, The Netherlands: Elsevier; 
Dalton, B.A., McFarland, C.D., Gengenbach, T.R., Griesser, H.J., Steele, J.G., Polymer surface chemistry and bone cell migration (1998) J Biomater Sci Polym Ed, 9, pp. 781-799; 
Healy, K.E., Thomas, C.H., Rezania, A., Kim, J.E., McKeown, P.J., Lom, B., Hockberger, P.E., Kinetics of bone cell organization and mineralization on materials with patterned surface chemistry (1996) Biomaterials, 17, pp. 195-208; 
Kasemo, B., Lausmaa, J., Material-tissue interfaces: The role of surface properties and processes (1995) Environ Health Perspect, 102, pp. 41-45; 
Von Recum, A.F., Van Kooten, T.G., The influence of micro-topography on cellular response and the implications for silicone implants (1995) J Biomater Sci Polym Ed, 7, pp. 181-198; 
Vogler, E.A., (1993) Interfacial chemistry in biomaterials science, Surfactant Science Series, vol. 49 (Wettability), 49, pp. 183-250. , Berg JC, editor. New York, USA: Marcel Dekker; 
Horbett, T.A., Weathersby, P.K., Adsorption of proteins from plasma to a series of hydrophilic-hydrophobic copolymers. I. Analysis with the in situ radionation technique (1981) J Biomed Mater Res, 15, pp. 403-423; 
Martson, M., Viljanto, J., Hurme, T., Saukko, P., Biocompatibility of cellulose sponge with bone (1998) Eur Surg Res, 30, pp. 426-432; 
Gross, U., Muller-Mai, C., Voigt, C., The tissue response on cellulose cylinders after implantation in the distal femur of rabbits (1992) Fourth World Biomaterials Congress, p. 192. , Berlin, Germany, April; 
Chauveaux, D., Barbie, C., Barthe, X., Baquey, C., Poustis, J., Biological behaviour of cellulosic materials after bone implantation: Preliminary results (1990) Clin Mater, 5, pp. 251-258; 
Ikada, Y., Biomedical applications of cellulose membranes (1989) Cellulose: structural and functional aspects, pp. 447-455. , Kennedy JF, Phillips GO, Williams PA, editors. Chichester, UK: Ellis Horwood; 
Miyamoto, T., Takahashi, S., Ito, H., Inagaki, H., Noishiki, Y., Tissue biocompatibility of cellulose and its derivatives (1989) J Biomed Mater Res, 23, pp. 125-133; 
Franz, G., Polysaccharides in pharmacy (1986) Adv Polym Sci, 76, pp. 1-30; 
Philipp, B., Bock, W., Schierbaum, F., Application of polysaccharides and their derivatives as supporting materials and auxiliary substances in medicine and nutrition (1979) J Polym Sci Polym Symp, 66, pp. 83-100; 
Galgut, P.N., Oxidized cellulose mesh. I. Biodegradable membrane in periodontal surgery (1990) Biomaterials, 11, pp. 561-564; 
Skoog, T., The use of periosteum and surgicel{\textregistered} for bone restoration in congenital clefts of the maxilla (1967) Scand J Plast Reconstr Surg, 1, pp. 113-130; 
Degenshein, G.A., Hurwitz, A., Ribacoff, S., Experience with regenerated oxidized cellulose (1963) NY State J Med, 63, pp. 2639-2643; 
Poustis, J., Baquey, C., Chauveaux, D., Mechanical properties of cellulose in orthopaedic devices and related environments (1994) Clin Mater, 16, pp. 119-124; 
Baquey, C., Barbi{\'e}, C., More, N., Rouais, F., Poustis, J., Chauveaux, D., In vivo study of the biostability of a cellulose material (1992) Fourth World Biomaterials Congress, p. 365. , Berlin, Germany, April; 
Brie, J., Lafage, M.H., Chauveaux, D., Gueroult, J.M., Poustis, J., Baquey, C., La cellulose macroporeuse: Un mat{\'e}riau potentiellement utilisable pour le comblement osseux. Biomat{\'e}riaux et organes artificiels (1992) Proceedings of the BIOMAT 92 Congress, , Bordeaux, France, November; 
Pommier, J.C., Poustis, J., Baquey, C., Chauveaux, D., Biocompatible, hydrophilic material. Method of manufacture and uses of same, , Fr Pat No. 8610331, 1986; Eur Pat No. 0256906 A1, 1987; US Pat No. 4904258, 1990; 
Barbi{\'e}, C., Chauveaux, D., Baquey, C., Poustis, J., Pommier, J.C., Morlier, R., Ducassou, D., {\'E}valuation des propriet{\'e}s mecaniques de mat{\'e}riaux cellulosiques et perspectives d{\textquoteright}application originales de ces mat{\'e}riaux en chirurgie orthop{\'e}dique (1987) Calcif Tiss Biomaterials, Proceedings of the BIOMAT 87 Congress, pp. 319-325. , Bordeaux, France, December; 
Li, S., Liu, Q., De Wijn, J., Wolke, J., Zhou, B., De Groot, K., In-vitro apatite formation on phosphorylated bamboo (1997) J Mater Sci: Mater Med, 8, pp. 543-549; 
Mucalo, M.R., Yokogawa, Y., Toryiama, M., Suzuki, T., Kawamoto, Y., Nagata, F., Nishizawa, K., Growth of calcium phosphate on surface-modified cotton (1995) J Mater Sci: Mater Med, 6, pp. 597-605; 
Mucalo, M.R., Yokogawa, Y., Suzuki, T., Kawamoto, Y., Nagata, F., Nishizawa, K., Further studies of calcium phosphate growth on phosphorylated cotton fibres (1995) J Mater Sci: Mater Med, 6, pp. 658-669; 
Granja, P.L., Baquey, C., Pouys{\'e}gu, L., Bareille, R., De J{\'e}so, B., Barbosa, M.A., (1996) Cellulose-based biomaterials for bone regeneration: Hemi-synthesis and characterisation. Polymers in Medicine and Surgery - PIMS{\textquoteright}96, pp. 73-80. , Glasgow, UK; 
Granja, P.L., Barbosa, M.A., Pouys{\'e}gu, L., De J{\'e}so, B., Baquey, C., Cellulose phosphates as biomaterials (1999) Frontiers in biomedical polymers applications 2, pp. 195-225. , Lancaster, PA, USA: Technomic Press; 
Granja, P.L., Pouys{\'e}gu, L., De J{\'e}so, B., Rouais, F., Baquey, C., Barbosa, M.A., Cellulose phosphates as biomaterials. Mineralization of chemically modified regenerated cellulose hydrogels (2001) J Mater Sci: Mater Med, , submitted for publication; 
Granja, P.L., De J{\'e}so, B., Bareille, R., Rouais, F., Baquey, C., Barbosa, M.A., (2001) Cellulose phosphates as biomaterials. In vitro biocompatibility studies, , submitted for publication; 
(1994) {\'E}valuation biologique des dispositifs m{\'e}dicaux. Partie 6: Essais concernant les effets locaux apr{\`e}s implantation; 
Wolf, E., Roser, K., Hahn, M., Wezkerling, H., Delling, G.G., Enzyme and immunohistochemistry on undecalcified bone and bone marrow biopsy after embedding in plastic: A new embedding method for routine application (1992) Virchow Archiv Pathol Anat Histopathol, 420, pp. 17-24; 
Van der Lubbe, H.B.M., Patchin, C., De Groot, K., A simple method for preparing thin (10 μm) histological sections of undecalcified plastic embedded bone with implant (1988) Saint Technol, 63, pp. 171-176; 
Daculsi, G., LeGeros, R.Z., Nery, E., Lynch, K., Transformation of biphasic calcium phosphate ceramics in vivo (1989) J Biomed Mater Res, 23, pp. 883-894; 
Charpak, G., Dominik, W., Zaganidis, N., Optical imaging of the spatial distribution of b-particles emerging from surfaces (1989) Proc Natl Acad Sci USA, 86, pp. 1741-1745; 
Charpak, G., Dominik, W., Fabre, J.P., Gaudaen, J., Sauli, F., Suzuki, M., Studies of light emission by continuously sensitive avalanche chambers (1988) Nucl Instrum Methods A, 269, pp. 142-148; 
Charpak, G., Dominik, W., Fabre, J.P., Santiard, J.C., Sauli, F., Solomey, N., Gaseous detectors with parallel electrodes and anode mesh planes (1979) Nucl Instrum Meth, 161, pp. 19-34; 
Daculsi, G., Passutti, N., Effect of the macroporosity for the osseous substitution of calcium phosphate ceramics (1990) Biomaterials, 11, pp. 86-87; 
Le Huec, J.C., Cl{\'e}ment, D., Brouillaud, B., Barthe, N., Dupuy, B., Foliguet, B., Basse-Cathalinat, B., Evolution of the local calcium content around irradiated β-tricalcium phosphate ceramic implants: In vivo study in the rabbit (1998) Biomaterials, 19, pp. 733-738; 
Bloebaum, D., Bachus, K., Momberger, N., Hofmann, A., Mineral apposition rates of human cancellous bone at the interface of porous coated implants (1994) J Biomed Mater Res, 28, pp. 537-544; 
Gross, U.M., Hard tissue histology (1999) Handbook of biomaterials evaluation: scientific, technical and clinical testing of implant materials. 2nd Ed., pp. 689-719. , von Recum AF, editor. MI, USA: Edwards Brothers, Ann Arbor Science; 
Sakkers, R.J.B., Dalmeyer, R.A.J., De Wijn, J.R., Van Blitterswijk, C.A., Use of bone-bonding hydrogel copolymers in bone: An in vitro and in vivo study of expanding PEO-PBT copolymers in goat femora (2000) J Biomed Mater Res, 49, pp. 312-318; 
Elliot, J.C., (1994) Structure and chemistry of the apatites and other calcium orthophosphates, Studies in Inorganic Chemistry Series, 18. , Amsterdam, The Netherlands: Elsevier; 
Aoki, H., (1994) Medical applications of hydroxyapatite, , Tokyo, Japan: Ishiyaku EuroAmerica; 
Goodman, S., Aspenberg, P., Effects of mechanical stimulation on the differentiation of hard tissues (1993) Biomaterials, 14, pp. 563-569; 
Klemm, D., Philipp, B., Heinze, T., Heinze, U., Wagenknecht, W., (1998) Comprehensive cellulose chemistry, vol. 2, functionalization of cellulose, 2, pp. 133-140. , Weinheim, Germany: Wiley-VCH Verlag. Chapter 4; 
Touey, G.P., Kingsport, T., Preparation of cellulose phosphates, , US Pat No. 2759924, 1956; 
Hayashi, T., Biodegradable polymers for biomedical uses (1994) Prog Polym Sci, 19, pp. 663-702; 
Fricain, J.C., Baquey, C., Dupuy, B., Resorption of corals implanted in diffusion chambers (1995) J Mater Sci: Mater Med, 6, pp. 680-684
},
	month = {2002///},
	pages = {971 - 980},
	abstract = {Femoral implantation of regenerated cellulose hydrogels revealed their biocompatibility, but a complete osseointegration could not be observed. Phosphorylation was therefore envisaged as the means to enhance cellulose bioactivity. In vitro studies showed that regenerated cellulose hydrogels promote bone cells attachment and proliferation but do not mineralize in acellular simulated physiological conditions. On the contrary, phosphorylated cellulose has shown an opposite behavior, by inducing the formation of a calcium phosphate layer in simulated physiological conditions, but behaving as a poor substrate for bone cells attachment and proliferation. In order to investigate the in vivo behavior of these materials, and assess the influence of mineralization induction ability vs. bone cells compatibility, unmodified and phosphorylated cellulose hydrogels were implanted in rabbits for a maximum period of 6 months and bone regeneration was investigated. Despite the difficulties arising from the retraction of cellulose hydrogels upon dehydration during the preparation of retrieved implants, histological observations showed no inflammatory response after implantation, with bone intra-spongious regeneration of cells and the integration of the unmodified as well as the phosphorylated cellulose implants. After a maximum implantation period of 6 months, histological observations, histomorphometry and the measurement of the amount of                        45Ca incorporated in the surrounding tissue indicated a slightly better osseointegration of phosphorylated cellulose, although no significant differences between the two materials were found. {\textcopyright} 2001 Elsevier Science Ltd. All rights reserved.
},
	keywords = {animal experiment, Animal implantation, animal tissue, Animalia, Animals, article, Biocompatibility, Biocompatible Materials, biomaterial, Biomaterials, Bone, bone cell, bone mineralization, Bone Regeneration, Calcium, calcium 45, Calcium Compounds, calcium phosphate, Calcium Radioisotopes, cell adhesion, cell proliferation, Cells, Cellulose, Cellulose phosphate, Chemical modification, controlled study, Dehydration, histology, hydrogel, Hydrogels, implant, in vivo study, Inflammation, materials testing, Microscopy, Electron, Scanning, morphometrics, nonhuman, Oryctolagus cuniculus, Osseointegration, Phosphates, Phosphorylation, priority journal, Prostheses and Implants, rabbit, Rabbits, Regenerated cellulose hydrogels},
	isbn = {01429612 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0037082737\&partnerID=40\&md5=faae93af84ab2762579f90bd2d64eefa},
	author = {Fricain, J.C. and Granja, P.L. and Barbosa, M.A. and De J{\'e}so, B. and Barthe, N. and Baquey, C.}
}
@article {2551,
	title = {CaO-P                    2O
                    5 glass ceramics containing bioactive phases: crystallisation and in vitro bioactivity studies
},
	journal = {Key Engineering MaterialsKey Eng Mat},
	volume = {192-195},
	year = {2001},
	note = {Conference code: 57760Cited By (since 1996): 3Export Date: 21 November 2012Source: ScopusCODEN: KEMAELanguage of Original Document: EnglishCorrespondence Address: Zhang, Y.; Instituto de Engenharia Biomedica, (INEB), Porto, PortugalReferences: Hench, L.L., (1991) J. Am. Ceram. Soc., 74, p. 1487;Kokubo, T., (1993) An Introduction to Bioceramics, p. 75. , L.L. Hench and J.Wilson, Eds., World Scientific Singapore; 
Abe, Y., Naruse, A., Hosoe, M., Fukui, H., Hasegawa, J., (1975) The 1975 Symposium of Biomaterials, p. 202. , Kyoto; 
Reaney, I.M., James, P.F., Lee, W.E., (1996) J. Am. Ceram. Soc., Science, 79, pp. 1934-1944; 
Kasuga, T., Abe, Y., (1998) J. Mater. Res., 13, p. 1; 
Vogel, J., Schulze, K.J., Reif, D., Hartmann, P., Platzbecker, U., Leuner, B., (1997) Bioceramics, 10, p. 57. , L. Sedel and C. Rey, Eds., Elsevier Science; 
Hosono, H., Zhang, Z., Abe, Y., (1989) J. Am. Ceram. Soc., 72, p. 1587; 
Zhang, Y., Santos, J.D., Journal of the European Ceramic Society, , In press
},
	month = {2001///},
	pages = {643 - 646},
	publisher = {Trans Tech Publ},
	address = {Bologna, Italy},
	abstract = {CaO-P                        2O
                        5 glass ceramics were prepared using a two-step heat treatment of nucleation and crystal growth using TiO
                        2 and Na
                        2O as nucleating agents. X-ray diffraction analysis demonstrated that bioactive and bioresorbable crystalline phases were precipitated in the microstructure, depending upon the relative content of the additives and of the CaO/P
                        2O
                        5 ratio of the glass. Ca
                        2P
                        2O
                        7 (β-DCP) was the main crystalline phase however β-Ca
                        3(PO
                        4)
                        2 (β-TCP), could also be formed by increasing the content of TiO
                        2 and the CaO/P
                        2O
                        5 ratio. Na and Ti-containing phases, CaTi
                        4(PO
                        4)
                        6, and/or NaTi
                        2(PO
                        4)
                        3, could also be assigned in the XRD spectra. In vitro testing in simulated body fluid (SBF) has proved the in vitro bioactivity of these glass ceramics with the formation of Ca-deficient apatite crystals, as demonstrated by Scanning Electron Microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) analyses.
},
	keywords = {Bioactivity, Biomaterials, Ceramic materials, Crystal growth, Crystal microstructure, Crystalline materials, Crystalline phase, Crystallization, glass, Lime, Nucleating agents, Nucleation, Phosphates, Scanning electron microscopy, Simulated body fluid, X ray diffraction analysis, X ray photoelectron spectroscopy},
	isbn = {10139826 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035154397\&partnerID=40\&md5=f2ba11b17e54efdc1e520c3dfaa6457e},
	author = {Zhang, Y. and Lopes, M.A. and Santos, J.D.}
}
@article {2536,
	title = {Cellulose phosphates as biomaterials. I. Synthesis and characterization of highly phosphorylated cellulose gels},
	journal = {Journal of Applied Polymer ScienceJ. Appl. Polym. Sci.},
	volume = {82},
	year = {2001},
	note = {Cited By (since 1996): 27Export Date: 21 November 2012Source: ScopusCODEN: JAPNAdoi: 10.1002/app.2193Language of Original Document: EnglishCorrespondence Address: Granja, P.L.; Laborat{\'o}rio de Biomateriais, Instituto de Engenharia Biomedica, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: pgranja@ibmc.up.ptReferences: Dumitriu, S., (1996) Polysaccharides in Medical Applications, , Marcel Dekker: New York;Franz, G., (1986) Adv Polym Sci, 76, p. 1; 
Ikada, Y., (1989) Cellulose: Structural and Functional Aspects, p. 447. , Kennedy J. F.; Phillips, G. O.; Williams, P. A., Eds.; Ellis Horwood: Chichester, UK, Chapter 59; 
Galleti, P.M., Colton, C.K., Lysaght, M.J., (1995) The Biomedical Engineering Handbook, p. 1898. , Bronzino, J. D., Ed.; CRC Press: Boca Raton, FL, Chapter 126; 
Hon, D.N.-S., (1996) Polysaccharides in Medicinal Applications, p. 87. , Dumitriu, S., Ed.; Marcel Dekker: New York, Chapter 4; 
Doelker, E., (1993) Adv Polym Sci, 107, p. 199; 
Miyamoto, T., Takahashi, S., Ito, H., Inagaki, H., Noishiki, Y., (1989) J Biomed Mater Res, 23, p. 125; 
Okajima, K., (1989) Cellulose: Structural and Functional Aspects, p. 439. , Kennedy, J. F.; Phillips, G. O.; Williams, P. A., Eds.; Ellis Horwood: Chichester, UK ; Chapter 58; 
Ahlgren, P., (1995) Nord Pulp Paper Res J, 1, p. 12; 
Dimemmo, L.M., Falkiewicz, M.J., (1985) Cellulose and Its Derivatives: Chemistry, Biochemistry and Applications, p. 511. , Kennedy, J. F.; Phillips, G. O.; Wedlock, D. J.; Williams, P. A., Eds.; Ellis Horwood: Chichester, UK, Chapter 47; 
Ross-Murphy, S.B., (1985) Cellulose Chemistry and Its Applications, p. 202. , Nevell, T. P.; Zeronian, S. H., Eds.; Ellis Horwood: Chichester, UK, Chapter 8; 
Steege, H., Philipp, B., Engst, R., Magister, G., Lewerenz, H.J., Bleyl, D., (1978) Tappi, 61, p. 101; 
Battista, O.A., (1971) Cellulose and Cellulose Derivatives (Part V), 5, p. 1265. , Bikales, N. M.; Segal, L., Eds.; High Polymers series, Wiley-Interscience: New York, Chapter 19; 
Matthew, I.R., Browne, R.M., Frame, J.W., Millar, B.G., (1995) Biomaterials, 16, p. 275; 
Finn, M.D., Schow, S.R., Schneiderman, E.D., (1992) J Oral Maxillofac Surg, 50, p. 608; 
Galgut, P.N., (1990) Biomaterials, 11, p. 561; 
Martson, M., Viljanto, J., Hurme, T., Laippala, P., Saukko, P., (1999) Biomaterials, 20, p. 1899; 
Poustis, J., Baquey, C., Chauveaux, D., (1994) Clin Mater, 16, p. 119; 
Gross, U., M{\"u}ller-Mai, C., Voigt, C., The tissue response on cellulose cylinders after implantation in the distal femur of rabbits (1992) Proceedings of the 4th World Biomaterials Congress, p. 192. , Berlin, Germany, April; 
Baquey, C., Barbi{\'e}, C., More, N., Rouais, F., Poustis, J., Chauveaux, D., (1992) Proceedings of the 4th World Biomaterials Congress, p. 365. , In vivo study of the biostability of a cellulose material, Berlin, Germany, April; 
Chauveaux, D., Barbi{\'e}, C., Barthe, X., Baquey, C., (1990) Poustis, J Clin Mater, 5, p. 251; 
Pommier, J.C., Poustis, J., Baquey, C., Chauveaux, D.Fr., Demande 8610331, 1986; Eur. Pat. 0256906 Al, 1987; U.S. Pat. 4,904,258, 1990Mizusawa, Y., Burke, J.R., (1996) J Pediatr Child Health, 32, p. 350; 
Parfitt, A.M., (1975) Clin Sci Mol Med, 49, p. 83; 
Pak, C.Y.C., Delea, C.S., Bartter, F.C., (1974) N Engl J Med, 290, p. 175; 
Dowd, V., Yon, R.J., (1992) J Chromatogr, 627, p. 145; 
Ermolenko, I.N., Lyubliner, I.P., Borodin, I.F., Orlyanskaya, V.F., Gavrilova, A.A., (1984) USSR Otkrytiya Izobret, p. 17; 
Sugihara, J., Imamura, T., Yanase, T., (1982) J Chromatogr, 229, p. 193; 
Longin, M.L., Buglov, E.D., Ermolenko, I.N., (1975) Vestsi Akad Navuk B SSR Ser Khim Navuk, p. 82; 
Shih, T.Y., Martin, M.A., (1974) Biochemistry, 13, p. 3411; 
Antonova, E.V., Mikhnovich, E.P., Shulutko, L.S., (1972) Probl Gematol Pereliv Krovi, 17, p. 25; 
Ermolenko, I.N., Lyubliner, I.P., (1971) Khim Teknhol Proizvod Tsellyul, p. 337; 
Holmquist, W.R., Schroeder, W.A., (1967) J Chromatogr, 26, p. 465; 
Kotake, Y., Ida, K., Kondo, S., Jpn. Pat. 10947(56), 1958Yokogawa, Y., Paz Reyes, J., Mucalo, M.R., Toriyama, M., Kawamoto, Y., Suzuki, T., Nishizawa, K., Kamayama, T., (1997) J Mater Sci Mater Med, 8, p. 407; 
Kato, E., Eika, Y., Ikada, Y., (1996) J Biomed Mater Res, 32, p. 687; 
Tretinnikov, O.N., Kato, K., Ikada, Y., (1994) J Biomed Mater Res, 28, p. 1365; 
Dalas, E., Kallistis, J., Koutsoukos, P.G., (1991) Langmuir, 7, p. 1822; 
Li, S., Liu, Q., De Wijn, J., Wolke, J., Zhou, B., De Groot, K., (1997) J Mater Sci Mater Mod, 8, p. 543; 
Mucalo, M.R., Yokogawa, Y., Toriyama, M., Suzuki, T., Kawamoto, Y., Nagata, F., Nishizawa, K., (1995) J Mater Sci Mater Med, 6, p. 597; 
Mucalo, M.R., Yokogawa, Y., Suzuki, T., Kawamoto, Y., Nagata, F., Nishizawa, K., (1995) J Mater Sci Mater Mod, 6, p. 658; 
Klemm, D., Philipp, B., Heinze, T., Heinze, U., Wagenknecht, W., (1998) Comprehensive Cellulose Chemistry: Functionalization of Cellulose, 2, p. 133. , Wiley-VCH Verlag: Weinheim, Germany, Chapter 4; 
Ishizu, A., (1991) Wood and Cellulose Chemistry, p. 757. , Hon, D. N.-S.; Shiraishi, N., Eds.; Marcel Dekker: New York, Chapter 16; 
Woodsworth, L.C., Daponte, D., (1985) Cellulose Chemistry and Applications, p. 344. , Nevell T. P.; Zeronian, S. H., Eds.; Ellis Horwood: Chichester, UK, Chapter 14; 
Reid, J.D., Mazzeno L.W., Jr., (1949) Ind Eng Chem, 41, p. 2828; 
Granja, P.L., Barbosa, M.A., Pouys{\'e}gu, L., De J{\'e}so, B., Baquey, C., (1999) Frontiers in Biomedical Polymers Applications, 2, p. 195. , Ottenbrite, R., Ed.; Technomic Press: Lancaster, PA; 
Granja, P.L., Baquey, C., Pouys{\'e}gu, L., Bareille, R., De J{\'e}so, B., Barbosa, M.A., (1996) Polymers in Medicine and Surgery (PIMS{\textquoteright}96), p. 73. , Glasgow, UK; 
Touey, G.P., Kingsport, T., U.S. Pat. 2,759,924, 1956Inoue, H., Baba, Y., Tsuhako, M., (1995) Chem Pharm Bull, 43, p. 677; 
Quin, L.D., Jankowski, S., WO 1994, 94/02226Wagenknecht, W., Nehls, I., Philipp, B., Schnabelrauch, M., Klemm, D., Hartmann, M., (1991) Acta Polym, 42, p. 554; 
Slotin, L.A., (1977) Synthesis, p. 737. , November; 
Inagaki, N., Nakamura, S., Asai, H., Katsuura, K., (1976) J Appl Polym Sci, 20, p. 2829; 
Vigo, T.L., Welch, C.M., (1974) Carbohydr Res, 32, p. 331; 
Towle, G.A., Whistler, R.L., (1972) Methods in Carbohydrate Chemistry, 6, p. 408. , Whistler, R. L., Ed.; Academic Press: New York ; Chapter 75; 
Katsuura, K., Nonaka, S., (1957) Sen-i-Gakkaishi, 13, p. 24; 
Nuessle, A.C., Ford, F.M., Hall, W.P., Lippert, A.L., (1956) Text Res J, 26, p. 32; 
Bertran, M.S., Dale, B.E., (1986) J Appl Polym Sci, 32, p. 4241; 
Reid, J.D., Mazzeno L.W., Jr., (1949) Ind Eng Chem, 41, p. 2831; 
(1982) Essais des eaux: Dosage des orthophosphates, des polyphosphates et du phosphore total (M{\'e}thode spectrom{\'e}trique), , AFNOR: Saint-Denis La Plaine, NF T 90-023; 
Segal, L., Creely, J.J., Martin A.E., Jr., Conrad, C.M., (1959) Text Res J, 29, p. 786; 
Nelson, M.L., O{\textquoteright}Connor, R.T., (1964) J Appl Polym Sci, 8, p. 1325; 
Klemm, D., Philipp, B., Heinze, T., Heinze, U., Wagenknecht, W., (1998) Comprehensive Cellulose Chemistry: Fundamentals and Analytical Methods, 1, p. 9. , Wiley-VCH Verlag: Weinheim, Germany, Chapter 2; 
Zeronian, S.H., (1985) Cellulose Chemistry and Its Applications, p. 159. , Novell, T. P.; Zeronian, S. H., Eds.; Ellis Horwood: Chichester, UK, Chapter 6; 
Klemm, D., Philipp, B., Heinze, T., Heinze, U., Wagenknecht, W., (1998) Comprehensive Cellulose Chemistry: Fundamentals and Analytical Methods, 1, p. 181. , Wiley-VCH Verlag: Weinheim, Germany, Chapter 3; 
Nyquist, R.A., Craver, C.D., (1977) The Coblentz Society Desk Book of Infrared Spectra, p. 399. , Craver C. D., Ed.; The Coblentz Society: French Village, MO; 
Nakamoto, K., (1977) Infrared and Raman Spectra of Inorganic and Coordination Compounds, 3rd ed., , Wiley-Interscience: New York; 
Kuptsov, A.H., Zhizhin, G.N., (1998) Handbook of fourier transform raman and infrared spectra of polymers, Physical Series Data, 45. , Elsevier: Amsterdam; 
Blackwell, J., (1977) ACS Symp Ser, 48, p. 206; 
Matthews, I.P., Gibson, C., Samuel, A.H., (1994) Clin Mater, 15, p. 191; 
Chamberlain, V.C., Lambert, B., Tang, F.-W., (1999) Handbook of Biomaterials Evaluation: Scientific, Technical, and Clinical Testing of Implant Materials, p. 253. , von Recum, A. F., Ed.; Taylor \& Francis: Philadelphia Chapter 15; 
Ahmed, A.U., Rapson, W.H., (1972) J Polym Sci Part A Polym Chem, 10, p. 1945; 
Phillips, G.O., Arthur J.C., Jr., (1985) Cellulose Chemistry and Its Applications, p. 290. , Novell, T. P.; Zeronian, S. H., Eds.; Ellis Horwood: Chichester, UK, Chapter 12; 
Larsson, P.T., Wickholm, K., Iversen, T., (1997) Carbohydr Res, 302, p. 19; 
Lai, Y.-Z., (1996) Chemical Modification of Lignocellulosic Materials, p. 35. , Hon, D. N.-S., Ed.; Marcel Dekker: New York, Chapter 3; 
Schultz, T.P., McGinnis, G.D., Bertran, M.S., (1985) J Wood Chem Technol, 5, p. 543; 
Roberts, G.A.F., (1992) Paper Chemistry, p. 9. , Roberts, J. C., Ed.; Blackie Academic \& Professional: Glasgow/London, Chapter 2; 
El-Saied, H., Hanna, A.A., Ibrahem, A.A., (1985) Indian Pulp Paper, 7-10, p. 24; 
Canet, D., (1996) Nuclear Magnetic Resonance: Concepts and Methods, , Wiley: Chichester, UK; 
Wu, Y., Glimcher, M.J., Rey, C., Ackerman, J.L., (1994) J Mol Biol, 244, p. 423; 
Sabesan, S., Neira, S., (1992) Carbohydr Res, 223, p. 169; 
Nehls, I., Loth, F., (1991) Acta Polym, 42, p. 233; 
Nehls, I., Wagenknecht, W., Philipp, B., Stscherbina, D., (1994) Prog Polym Sci, 19, p. 29; 
Buchanan, G.W., Preusser, S.H., Webb, V.L., (1984) Can J Chem, 62, p. 1308; 
Christofides, J.C., Davies, D.B., (1983) J Am Chem Soc, 105, p. 5099; 
Beierbeck, H., Saunders, J.K., (1976) Can J Chem, 54, p. 2985; 
Powlowski, W.P., (1987) J Macromol Sci Chem, 25, p. 3355; 
Lowman, D.W., (1998) Cellulose Derivatives: Modification, Characterization, and Nanostructures, p. 131. , Heinze, T. J.; Glasser, W. G., Eds.; ACS Symposium Series 688; American Chemical Society: Washington, DC, Chapter 10; 
Nehls, I., Wagenknecht, W., Philipp, B., (1995) Cellul Chem Technol, 29, p. 243; 
Nehls, I., Philipp, B., Wagenknecht, W., (1995) Cellulose and Cellulose Derivatives: Physico-Chemical Aspects and Industrial Applications, p. 153. , Kennedy, J. F.; Phillips, G. O.; Williams, P. A., Eds.; Woodhead Publishing: Cambridge, UK, Chapter 20; 
Hoshino, M., Takai, M., Fukuda, K., Imura, K., Hayashi, J., (1989) J Polym Sci Part A Polym Chem, 27, p. 2083; 
Takahashi, S.I., Fujimoto, T., Barua, B.M., Miyamoto, T., Inagaki, H., (1986) J Polym Sci Part A Polym Chem, 24, p. 2981; 
Miyamoto, T., Sato, Y., Shibata, T., Inagaki, H., Tanahashi, M., (1984) J Polym Sci Part A Polym Chem, 22, p. 2363; 
VanderHart, D.L., Hyatt, J.A., Atalla, R.H., Tirumalai, V.C., (1996) Macromolecules, 29, p. 730; 
Horii, F., Hirai, F., Kitamaru, R., (1992) Polym Bull, 8, p. 163; 
Atalla, R.H., VanderHart, D.L., (1984) Science, 223, p. 283; 
Ganapathy, S., Badiger, M.V., Rajamohanan, P.R., Mashelkar, R.A., (1992) Macromolecules, 25, p. 4255; 
Horii, F., (1989) Nuclear Magnetic Resonance in Agriculture, p. 311. , Pfeffer, P. E.; Gerasimovicz, W. V., Eds.; CRC Press: Boca Raton, FL; 
Horii, F., Hirai, A., Kitamaru, R., (1986) Macromolecules, 19, p. 930; 
Okada, K., Yokogawa, Y., Kameyama, T., Kato, K., Kawamoto, Y., Nishizawa, K., Nagata, F., Okuyama, M., (1997) Bioceramics, 10, p. 329. , Sedel, L.; Rey, C., Eds.; Elsevier: Amsterdam; 
Yalpani, M., (1992) Carbohydr Polym, 19, p. 35; 
Nishi, N., Ebina, A., Nishimura, S., Tsutsumi, A., Hasegawa, O., Tokura, S., (1986) Int J Biol Macromol, 8, p. 311
},
	month = {2001///},
	pages = {3341 - 3353},
	abstract = {The present study concerns the investigation of a material expected to be biocompatible and able to promote bone regeneration. For this purpose, cellulose was chemically modified by phosphorylation. Once implanted, phosphorylated cellulose could promote the formation of calcium phosphates, thus having closer resemblance to bone functionality. In a previous investigation, the obtention and the preliminary characterization of cellulose phosphate gels were reported. In the present study, the synthesis by the H                        3PO
                        4/P
                        2O
                        5/Et
                        3PO 
                        4/hexanol method was optimized in terms of reaction parameters. The structure of materials was investigated by FTIR, Raman, and solid-state 
                        31P- and 
                        13C-NMR spectroscopic studies, and X-ray diffraction. Water swelling and stability to sterilization by gamma-radiation were also assessed. It was demonstrated that the present method allows highly phosphorylated cellulose derivatives to be obtained. Cellulose triphosphates gels are described here for the first time. Products obtained were poorly crystallized monoesters, apparently not significantly affected by gamma sterilization and showed a high water swelling capability. Chemical bonding was confirmed by FTIR, Raman, and both 
                        31P- and 
                        13C-NMR spectroscopies. It was also shown that the H
                        3PO
                        4/P
                        2O
                        5/Et
                        3PO 
                        4/hexanol method provides a versatile and interesting alternative route to some of the more widely used techniques for the phosphorylation of cellulose. {\textcopyright} 2001 John Wiley \&amp; Sons, Inc. J. Appl. Polym. Sci.
},
	keywords = {13C-NMR, 2O, 3PO, 4/hexanol method, 4/P, 5/Et, Biomaterials, Cellulose derivatives, Cellulose gels, Cellulose triphosphates, Chemical bonds, Fourier transform infrared spectroscopy, Gels, H, medical textile, Nuclear magnetic resonance spectroscopy, Phosphorylation, Raman scattering, Sterilizers, Synthesis (chemical), X ray diffraction analysis},
	isbn = {00218995 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035923986\&partnerID=40\&md5=7a58f84a4d86e26d35d7d90d44aecb63},
	author = {Granja, P.L. and Pouysgu, L. and Ptraud, M. and De Jso, B. and Baquey, C. and Barbosa, M.A.}
}
@article {2537,
	title = {Cellulose phosphates as biomaterials. II. Surface chemical modification of regenerated cellulose hydrogels},
	journal = {Journal of Applied Polymer ScienceJ. Appl. Polym. Sci.},
	volume = {82},
	year = {2001},
	note = {Cited By (since 1996): 16Export Date: 21 November 2012Source: ScopusCODEN: JAPNAdoi: 10.1002/app.2194Language of Original Document: EnglishCorrespondence Address: Granja, P.L.; Laborat{\'o}rio de Biomaterials, Instituto de Engenharia Biomedica, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: pgranja@ibmc.up.ptReferences: Williams, D.F., (1995) Med Der Technol, 6, p. 6;Gebelein, C.G., (1990) Biomimetic Polymers, , Plenum Press: New York; 
Hoffman, A.S., (1996) Biomaterials Science: An Introduction to Materials in Medicine, p. 124. , Ratner, B. D.; Hoffman, A. S.; Schoen, F. J.; Lemons, J. F., Eds.; Academic Press: San Diego, Chapter 2; 
Kasemo, B., Lausmaa, J., (1995) Environ Health Perspect, 102, p. 41; 
Ratner, B.D., (1980) J Biomed Mater Res, 14, p. 665; 
Poustis, J., Baquey, C., Chauveaux, D., (1994) Clin Mater, 16, p. 119; 
Gross, U., M{\"u}ller-Mai, C., Voigt, C., (1992) Proceedings of the 4th World Biomaterials Congress, p. 192. , The Tissue Response on Cellulose Cylinders After Implantation in the Distal Femur of Rabbits, Berlin, Germany, April; 
Baquey, C., Barbi{\'e}, C., More, N., Rouais, F., Poustis, J., Chauveaux, D., (1992) Proceedings of the 4th World Biomaterials Congress, p. 365. , In Vivo Study of the Bio-Stability of a Cellulose Material, Berlin, Germany, April; 
Chauveaux, D., Barbi{\'e}, C., Barthe, X., Baquey, C., Poustis, J., (1990) Clin Mater, 5, p. 251; 
Pommier, J.C., Poustis, J., Baquey, C., Chauveaux, D., Fr. Demande 8610331. 1986Eur. Pat. 0256906 A1, 1987U.S. Pat. 4,904,258, 1990Imbert, E., Letourneur, D., Jozefowicz, M., (1997) J Biomed Mater Res, 34, p. 417; 
Dowd, V., Yon, R.J., (1992) J Chromatogr, 627, p. 145; 
Kennedy, J.F., Cabral, J.M.S., (1983) Solid Phase Biochemistry: Analytical Synthetic Aspects, p. 253. , Scoutch, W. H., Ed. Wiley: New York, Chapter 7; 
Sugihara, J., Imamura, T., Yanase, T., (1982) J Chromatogr, 229, p. 193; 
Shih, T.Y., Martin, M.A., (1974) Biochemistry, 13, p. 3411; 
Holmquist, W.R., Schroeder, W.A., (1967) J Chromatogr, 26, p. 465; 
Thomaidis, T.S., Fidalgo, B.V., Harshman, S., Najjar, V.A., (1967) Biochemistry, 6, p. 3369; 
Granja, P.L., Pouys{\'e}gu, L., P{\'e}traud, M., De J{\'e}so, B., Baquey, C., Barbosa, M.A., (2001) J Appl Polym Sci, 82, p. 3341; 
Granja, P.L., Barbosa, M.A., Pouys{\'e}gu, L., De J{\'e}so, B., Baquey, C., (1999) Frontiers in Biomedical Polymers Applications, 2, p. 195. , Ottenbrite, R., Ed. Technomic Press: Lancaster, PA; 
Granja, P.L., Baquey, C., Pouys{\'e}gu, L., Bareille, R., De J{\'e}so, B., Barbosa, M.A., (1996) Polymers in Medicine and Surgery, PIMS{\textquoteright}96, p. 73. , Glasgow, UK; 
Klemm, D., Philipp, B., Heinze, T., Heinze, U., Wagenknecht, W., (1998) Comprehensive Cellulose Chemistry: Fundamentals and Analytical Methods, 1, p. 9. , Wiley-VCH Verlag: Weinheim, Germany, Chapter 2; 
Zeronian, S.H., (1985) Cellulose Chemistry and Its Applications, p. 159. , Nevell, T.P. Zeronian, S.H., Eds. Ellis Horwood: Chichester, UK, Chapter 6; 
Granja, P.L., Pouys{\'e}gu, L., De J{\'e}so, B., Rouais, F., Baquey, C., Barbosa, M.A., J Appl Polym Sci, , to appear; 
Ratner, B.D., Castner, D., (1997) Surface Analysis: The Principal Techniques, p. 43. , Vickerman, J.C., Ed. Wiley: Chichester, UK, Chapter 3; 
Dupraz, A., Nguyen, T.-P., Richard, M., Daculsi, G., Passuti, N., (1999) Biomaterials, 20, p. 663; 
Nguyen, T.-P., Dupraz, A., (1996) J Biomater Sci Polym Ed, 8, p. 141; 
Hanawa, T., Ota, M., (1991) Biomaterials, 12, p. 767; 
Morra, M., Volpe, C.D., (1998) J Biomed Mater Res, 42, p. 473; 
Garbassi, F., Morra, M., Occhiello, E., (1994) Polymer Surfaces: From Physics to Technology, p. 161. , Wiley: New York, Chapter 4; 
Vogler, E.A., (1993) Surfactant Science Series 49 (Wettability), p. 183. , Marcel Dekker: New York; 
Good, R.J., (1992) J Adhes Sci Technol, 6, p. 1269; 
Good, R.J., (1979) Surface and Colloid Science, 11, p. 1. , Good, R.J. Stromberg, R.R., Eds. Plenum Press: New York; 
Von Recum, A.F., Brown, C.E., Shannon, C.E., LaBerge, M., (1999) Handbook of Biomaterials Evaluation: Scientific, Technical, and Clinical Testing of Implant Materials, 2nd ed., p. 227. , von Recum, A.F., Ed. Edwards Brothers: Ann Arbor, MI, Chapter 13; 
Boyan, B.D., Batzer, R., Kieswetter, K., Liu, Y., Cochran, D.L., Szmuckler-Moncler, S., Dean, D.D., Schwartz, Z., (1998) J Biomed Mater Res, 39, p. 77; 
Richards, R.G., (1996) Injury, 27, pp. SC38; 
Brunette, D.M., (1988) Surface Characterization of Biomaterials, p. 203. , Ratner, B.D., Ed. Elsevier Science: Amsterdam; 
Kuptsov, A.H., Zhizhin, G.N., (1998) Handbook of Fourier Transform Raman and Infrared Spectra of Polymers, Physical Series Data, 45. , Elsevier: Amsterdam; 
Nyquist, R.A., Craver, C.D., (1977) The Coblentz Society Desk Book of Infrared Spectra, p. 399. , Craver, C.D., Ed. The Coblentz Society: French Village, MO; 
Nakamoto, K., (1977) Infrared and Raman Spectra of Inorganic and Coordination Compounds, 3rd ed., , Wiley-Interscience: New York; 
Klemm, D., Philipp, B., Heinze, T., Heinze, U., Wagenknecht, W., (1998) Comprehensive Cellulose Chemistry: Fundamentals and Analytical Methods, 1, p. 181. , Wiley-VCH Verlag: Weinheim, Germany, Chapter 3; 
Matthews, I.P., Gibson, C., Samuel, A.H., (1994) Clin Mater, 15, p. 191; 
Chamberlain, V.C., Lambert, B., Tang, F.-W., (1999) Handbook of Biomaterials Evaluation: Scientific, Technical, and Clinical Testing of Implant Materials, p. 253. , von Recum, A.F., Ed. Taylor \& Francis: Philadelphia, Chapter 15; 
Phillips, G.O., Arthur J.C., Jr., (1985) Cellulose Chemistry and Its Applications, p. 290. , Nevell, T.P. Zeronian,S.H., Eds.; Ellis Horwood: Chichester, UK, Chapter 12; 
Ahmed, A.U., Rapson, W.H., (1972) J Polym Sci Part A Polym Chem, 10, p. 1945; 
Canet, D., (1996) Nuclear Magnetic Resonance: Concepts and Methods, , Wiley: Chichester, UK; 
Vogel, J., Russel, C., Gunther, G., Hartmann, P., Vizethum, F., Bergner, N., (1996) J Mater Sci Mater Med, 7, p. 495; 
Wu, Y., Glimcher, M.J., Rey, C., Ackerman, J.L., (1994) J Mol Biol, 244, p. 423; 
Rothwell, W.P., Waugh, J.S., Yesinowski, J.P., (1980) J Am Chem Soc, 102, p. 2637; 
Yokogawa, Y., Paz Reyes, J., Mucalo, M.R., Toriyama, M., Kawamoto, Y., Suzuki, T., Nishizawa, K., Kamayama, T., (1997) J Mater Sci Mater Med, 8, p. 407
},
	month = {2001///},
	pages = {3354 - 3365},
	abstract = {Cellulose regenerated by the viscose process was previously investigated as an implantable material in orthopedic surgery. It was envisaged to take advantage not only of its good matching with mechanical properties of bone but also of its hydroexpansivity, therefore allowing a satisfactory fixation to hard tissue. Both the osteoconduction and the lack of ostcoinduction of this material were demonstrated. Grafting of phosphate groups was then envisaged as the means to render cellulose more suitable for orthopedic applications by enhancing its bioactivity. In the present work, the previously optimized phosphorylation reaction was successfully adapted to the surface modification of regenerated cellulose. Modified materials were characterized by XPS, FTIR, and                        31P MAS NMR spectroscopic studies, and contact angle measurements, revealing the chemical bond between phosphate groups and cellulose, as well as the hydrophilic nature of phosphorylated materials, which increases with increasing phosphate contents. Water swelling and resistance to gamma sterilization were assessed as well, showing that phosphorylated materials swell considerably in water and were not affected when sterilization was carried out under a nitrogen atmosphere. The increase in surface roughness attributed to chemical modification was demonstrated through laser rugosimetry measurements. {\textcopyright} 2001 John Wiley \&amp; Sons, Inc. J. Appl. Polym. Sci.
},
	keywords = {2O, 3PO, 4/hexanol method, 4/P, 5/Et, Biomaterials, Cellulose derivatives, Cellulose phosphates, Chemical bonds, Chemical modification, Energy dispersive spectroscopy, Fourier transform infrared spectroscopy, H, Hydrogels, Phosphorylation, Regenerated cellulose hydrogels, Sterilization (cleaning), Surface modification, Surface roughness, X ray photoelectron spectroscopy},
	isbn = {00218995 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035923980\&partnerID=40\&md5=5663bc99f91ce47534779e826f87ed97},
	author = {Granja, P.L. and Pouysgu, L. and Ptraud, M. and De Jso, B. and Baquey, C. and Barbosa, M.A.}
}
@article {2542,
	title = {On combining classifiers using sum and product rules},
	journal = {Pattern Recognition LettersPattern Recogn. Lett.},
	volume = {22},
	year = {2001},
	note = {Cited By (since 1996): 51Export Date: 21 November 2012Source: Scopusdoi: 10.1016/S0167-8655(01)00073-3Language of Original Document: EnglishCorrespondence Address: Alexandre, L.A.; INEB-Inst. de Engenharia Biomedica, Rua Dr. Roberto Frias s/n, Porto 4200-465, Portugal; email: lfbaa@noe.ubi.ptReferences: Alexandre, L., Campilho, A., Kamel, M., Combining independent and unbiased classifiers using weighted average (2000) Proc. 15th Internat. Conf. on Pattern Recognition, 2. , IEEE Press, Barcelona, Spain;Bishop, C., (1995) Neural Networks for Pattern Recognition, , Oxford University Press, Oxford; 
Blake, C., Keogh, E., Merz, C., (1998) UCI Repository of Machine Learning Databases, , http://www.ics.uci.edu/~mlearn/MLRepository.html; 
Demuth, H., Beale, M., (1998) Neural Network Toolbox User{\textquoteright}s Guide, , The MathWorks, Inc; 
Hansen, L., Salamon, P., Neural network ensembles (1990) IEEE Trans. PAMI, 12 (10), pp. 993-1001; 
Hashem, S., Optimal linear combinations of neural networks (1997) Neural Networks, 10 (4), pp. 599-614; 
Kittler, J., Hatef, M., Duin, R., Combining classifiers (1996) Proc. Internat. Conf. on Pattern Recognition{\textquoteright}96; 
Kittler, J., Hatef, M., Duin, R., Matas, J., On combining classifiers (1998) IEEE Trans. PAMI, 20 (3), pp. 226-239; 
Opitz, D., Maclin, R., Popular ensemble methods: An empirical study (1999) J. Artif. Intell. Res, 11, pp. 169-198; 
Richard, M., Lippmann, R., Neural network classifiers estimate Bayesian a posteriori probabilities (1991) Neural Comput, 3, pp. 461-483; 
Rogova, G., Combining the results of several neural network classifiers (1994) Neural Networks, 7 (5), pp. 777-781; 
Tax, D., Duin, R., Breukelen, M., Comparison between product and mean classifier combination rules (1997) Workshop on Statistical Techniques in Pattern Recognition, , Prague Czech Republic; 
Ueda, N., Optimal linear combination of neural networks for improving classification performance (2000) IEEE Trans. PAMI, 22 (2), pp. 207-215
},
	month = {2001///},
	pages = {1283 - 1289},
	abstract = {This paper presents a comparative study of the performance of arithmetic and geometric means as rules to combine multiple classifiers. For problems with two classes, we prove that these combination rules are equivalent when using two classifiers and the sum of the estimates of the a posteriori probabilities is equal to one. We also prove that the case of a two class problem and a combination of two classifiers is the only one where such equivalence occurs. We present experiments illustrating the equivalence of the rules under the above mentioned assumptions. {\textcopyright} 2001 Elsevier Science B.V. All rights reserved.},
	keywords = {artificial neural network, Classification, Classifier fusion, Combinatorial mathematics, Combining classifiers, Equivalence classes, Estimation, K nearest-neighbours, Multiple classifiers, Neural networks, Pattern recognition, probability},
	isbn = {01678655 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034875843\&partnerID=40\&md5=becfe9c0c4a7d97f7bbaf33f3059ef8f},
	author = {Alexandre, L.A. and Campilho, A.C. and Kamel, M.}
}
@article {2540,
	title = {Comparative effects of nacre water-soluble matrix and dexamethasone on the alkaline phosphatase activity of MRC-5 fibroblasts},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {57},
	year = {2001},
	note = {Cited By (since 1996): 20Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/1097-4636(200111)57:2<306::AID-JBM1172>3.0.CO;2-HPubMed ID: 11484195Language of Original Document: EnglishCorrespondence Address: Lopez, E.; Lab. de Physiol. Gen. et Comparee, UMR CNRS 8572, Museum National d{\textquoteright}Histoire Naturelle, 7 rue Cuvier, 75231 Paris Cedex 05, France; email: lopez@mnhn.frChemicals/CAS: Alkaline Phosphatase, EC 3.1.3.1; BMP2 protein, human; Bmp2 protein, rat; bone morphogenetic protein 2; Bone Morphogenetic Proteins; Dexamethasone, 50-02-2; Glucocorticoids; Transforming Growth Factor betaReferences: Lopez, E., Vidal, B., Berland, S., Camprasse, S., Camprasse, G., Silve, C., Demonstration of the capacity of nacre to induce bone formation by human osteoblasts maintained in vitro (1992) Tissue Cell, 24, pp. 667-679;Lopez, E., Berland, S., Le Faou, A., Nacre, osteogenic and osteoinductive properties (1994) Bull Inst Oc{\'e}an Monaco, 14, pp. 49-57; 
Silve, C., Lopez, E., Vidal, B., Smith, D.C., Camprasse, S., Camprasse, G., Couly, G., Nacre initiates biomineralization by human osteoblasts maintained in vitro (1992) Calcif Tissue Int, 51, pp. 363-369; 
Atlan, G., Balmain, N., Berland, S., Vidal, B., Lopez, E., Reconstruction of human maxillary defects with nacre powder: Histological evidence for bone regeneration (1997) C R Acad Sci III, 320, pp. 253-258; 
Atlan, G., Delattre, O., Berland, S., Le Faou, A., Nabias, G., Cot, D., Lopez, E., Interface between bone and nacre implants in sheep (1999) Biomaterials, 20, pp. 1017-1022; 
Lamghari, M., Almeida, M.J., Berland, S., Huet, H., Laurent, A., Milet, C., Lopez, E., Stimulation of bone marrow cells and bone formation by nacre: In vivo and in vitro studies (1999) Bone, 25 S, pp. 91S-94S; 
Westbroek, P., Marin, F., A marriage of bone and nacre (1998) Nature, 392, pp. 861-862; 
Le Faou, A., Berland, S., Lallier, F., Lopez, E., Osteoinductive effects induced by nacre implanted in subcutaneous pouches in rat (1995) Calcif Tissue Int, 56 A, p. 156; 
Le Faou, A., Berland, S., Lallier, F., Lopez, E., Local regulation of the osteoinductive process induced by nacre in connective tissues (1996) Ann Endoc, 57 (SUPPL. 4A), p. 75; 
Aubin, J.E., Liu, F., Malaval, L., Gupta, A.K., Osteoblast and chondroblast differentiation (1995) Bone, (SUPPL. 2), pp. S77-S83; 
Huggins, C.B., Urist, M.R., Dentin matrix transformation: Rapid induction of alkaline phosphatase and cartilage (1970) Science, 167, pp. 896-897; 
Reddi, A.H., Huggins, C.B., Biochemical sequences in the transformation of normal fibroblasts in adolescent rats (1972) Proc Natl Acad Sci USA, 69, pp. 1601-1605; 
Glowacki, J., Mulliken, J.B., Demineralized bone implants (1985) Clin Plast Surg, 12 (2), pp. 233-241; 
Katagiri, T., Yamaguchi, A., Ikeda, T., Yoshiki, S., Wozney, J.M., Rosen, V., Wang, E.A., Suda, T., The nonosteogenic mouse pluripotent cell line, C3H10T1/2, is induced to differentiate into osteobtastic cells by recombinant human bone morphogenetic protein-2 (1990) Biochem Biophys Res Commun, 172, pp. 295-299; 
McCulloch, C.A.G., Tenenbaum, H.C., Dexamethasone induces proliferation and terminal differentiation of osteogenic cells in tissue culture (1986) Anat Rec, 215, pp. 397-402; 
Bellows, C.G., Aubin, J.E., Heersche, J.N.M., Physiological concentrations of glucocorticoids stimulate formation of bone nodules from isolated rat calvaria cells in vitro (1987) Endocrinology, 121, pp. 1985-1992; 
Bellows, C.G., Aubin, J.E., Determination of numbers of osteoprogenitors present in isolated fetal calvarial cells in vitro (1989) Dev Biol, 133, pp. 8-13; 
Bhargava, U., Bar-Lev, M., Bellows, C.G., Aubin, J.E., Ultrastructural analyses of bone nodules formed in vitro by isolated fetal rat calvaria cells (1988) Bone, 9, pp. 155-163; 
Wiren, K.M., Rodan, G.A., Dissociation between the effects of glucocorticosteroids on cell spreading and phenotypic expression (1985) The chemistry and biology of mineralized tissues, pp. 320-325. , Butler WT, editor. Birmingham, AL: EBSCO Media; 
Kasugai, S., Todescan, R., Nagata, T., Yao, K.-L., Butler, W.T., Sodek, J., Expression of bone matrix proteins associated with mineralized tissue formation by adult rat bone marrow cells in vitro: Inductive effects of dexamethasone on the osteoblastic phenotype (1991) J Cell Physiol, 147, pp. 111-120; 
Chen, T.L., Bates, R.L., Dudley, A., Hammonds, R.G., Amento, E.P., Bone morphogenetic protein-2b stimulation of growth and osteogenic phenotypes in rat osteobtast-like cells: Comparison with TGF-β1 (1991) J Bone Miner Res, 6, pp. 1387-1393; 
Hiraki, Y., Inoue, H., Shigeno, C., Sanma, Y., Bentz, H., Rosen, D.M., Asada, A., Suzuki, F., Bone morphogenetic proteins (BMP-2 and BMP-3) promote growth and expression of the differentiated phenotype of rabbit chondrocytes and osteoblastic MC3T3-E1 cells in vitro (1991) J Bone Miner Res, 6, pp. 1373-1385; 
Rosen, V., Thies, R.S., The BMP proteins in bone formation and repair (1992) Trends Genet, 8, pp. 97-102; 
Thies, R.S., Bauduy, M., Ashton, B.A., Kurtzberg, L., Wozney, J.M., Rosen, V., Recombinant human bone morphogenetic protein-2 induces osteoblastic differentiation in W-20-17 stromal cells (1992) Endocrinology, 130, pp. 1318-1324; 
Urist, M.R., Iwata, H., Ceccotti, P.L., Dorfman, R.L., Boyd, S.D., McDowell, R., Chien, C., Bone morphogenesis in implants of insoluble bone gelatin (1973) Proc Natl Acad Sci USA, 70, pp. 3511-3515; 
Almeida, M.J., Milet, C., Peduzzi, J., Pereira, L., Haigle, J., Barth{\'e}lemy, M., Lopez, E., The effect of water-soluble matrix fraction extracted from the nacre of Pinctada maxima on the alkaline phosphatase activity of cultured fibroblasts (2000) J Exp Zool, 288, pp. 327-334; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats (1988) Cell Tissue Res, 254, pp. 317-330; 
Owen, M., Marrow stromal stem cells (1988) J Cell Sci, (SUPPL. 10), pp. 63-76; 
Triffitt, J.T., Initiation and enhancement of bone formation (1987) Acta Orthop Scand, 58, pp. 673-684; 
Triffitt, J.T., The stem cell of the osteoblast (1996) Principles of bone biology, pp. 39-50. , Bilezikian JP, Raisz LG, Rodan GA, editors. New York: Academic Press; 
Wharton, S.A., Hipkiss, A.R., Degradation of peptides and proteins of different sizes by homogenates of human MRC5 lung fibroblasts (1985) FEBS Lett, 184 (2), pp. 249-253; 
Harada, K., Oida, S., Sasaki, S., Chondrogenesis and osteogenesis of bone marrow-derived cells by bone-inductive factor (1988) Bone, 9, pp. 177-183; 
Tucker, R.F., Shipley, G.D., Moses, H.L., Growth inhibitor from BSC-1 cells closely related to platelet type β transforming factor (1984) Science, 226, pp. 705-707; 
Noda, M., Rodan, G.A., Type-β transforming growth factor inhibits proliferation and expression of alkaline phosphatase in murine osteoblast-like cells (1986) Biochem Biophys Res Commun, 140, pp. 56-65; 
Tintut, Y., Parhami, F., Bostr{\"o}m, K., Jackson, S., Demer, L., cAMP stimulates osteoblast-like differentiation of calcifying vascular cells (1998) J Biol Chem, 273, pp. 7547-7553; 
Rickard, D.J., Sullivan, T.A., Shenker, B.J., Leboy, P.S., Kazhdan, I., Induction of rapid osteoblast differentiation in rat bone marrow stromal cell cultures by dexamethasone and BMP-2 (1994) Dev Biol, 161, pp. 218-228; 
Coelho, M.J., Fernandes, M.H., Human bone cell cultures in biocompatibility testing. Part II. Effect of ascorbic acid, β-glycerophosphate and dexamethasone on osteoblastic differentiation (2000) Biomaterials, 21, pp. 1095-1102; 
Green, S., Chambon, P., A superfamily of potentially oncogenic hormone receptors (1986) Nature, 324, pp. 615-617; 
Grigoriadis, A.E., Heersche, J.N.M., Aubin, J.E., Differentiation of muscle, fat, cartilage and bone from progenitor cells present in a bone-derived clonal cell population: Effect of dexamethasone (1988) J Cell Biol, 106, pp. 2139-2151; 
Leboy, P.S., Beresford, J.N., Devlin, C., Owen, M.E., Dexamethasone induction of osteoblast mRNAs in rat marrow stromal cell cultures (1991) J Cell Physiol, 146, pp. 370-378; 
Guerriero V., Jr., Florini, J.R., Dexamethasone effects on myoblast proliferation and differentiation (1980) Endocrinology, 106, pp. 1198-1202; 
Maor, G., Silbermann, M., Supraphysiological concentrations of dexamethasone induce elevation of calcium uptake and depression of [ 3H]-thymidine incorporation into DNA in cartilage in vitro (1986) Calcif Tissue Int, 39, pp. 284-290; 
Urist, M.R., Bone morphogenetic protein in biology and medicine (1996) Bone morphogenetic proteins: Biology, biochemistry and reconstructive surgery, pp. 7-27. , Lindholm TS, editor. Austin, TX: RG Landes Company; 
Wozney, J.M., Rosen, V., Byrne, T., Celeste, A.J., Moutsatsos, I., Wang, E.A., Growth factor influencing bone development (1990) J Cell Sci, 97 (SUPPL. 13), pp. 149-156; 
Takuwa, Y., Ohse, C., Wang, E.A., Wozney, J.M., Yamashita, K., Bone morphogenetic protein-2 stimulates alkaline phosphatase activity and collagen synthesis in cultured osteoblastic cells, MC3T3 (1991) Biochem Biophys Res Commun, 174, pp. 96-101; 
Jortikka, L., Target cells and receptors for bone morphogenetic protein (1996) Bone morphogenetic proteins: Biology, biochemistry and reconstructive surgery, pp. 65-73. , Lindholm TS, editor. Austin, TX: RG Landes Company; 
Canalis, E., Centrella, M., Urist, M.R., Effect of partially purified bone morphogenetic protein on DNA synthesis and cell replication in calvarial and fibroblast cultures (1985) Clin Orthop, 198, pp. 289-294; 
Centrella, M., Casinghino, S., Kim, J., Pham, T., Rosen, V., Wozney, J., McCarthy, T.L., Independent changes in type I and type II receptors for transforming growth factor β induced by bone morphogenetic protein 2 parallel expression of the osteoblast phenotype (1995) Mol Cell Biol, 15, pp. 3273-3281; 
Vukicevic, S., Luyten, F.P., Redi, A.H., Stimulation of the expression of osteogenic and chondrogenic phenotypes in vitro by osteogenin (1989) Proc Natl Acad Sci USA, 86, pp. 8793-8797
},
	month = {2001///},
	pages = {306 - 312},
	abstract = {In this study we demonstrate, for the first time, that dexamethasone and BMP-2 stimulated alkaline phosphatase (ALP) activity in MRC-5 fibroblasts, a cell line derived from human fetal lung. Previously we reported that the water-soluble matrix (WSM) of nacre obtained from the inner shell layer of the oyster Pinctada maxima, promoted an increase in ALP activity that was dose-dependent. In this work, we show that the effect of WSM is also time-dependent. As a comparison, the effect of WSM was also tested in bone marrow stromal cells because marrow and other bone surface-derived osteoblast stem cells have the inherent direct potential for osteogenesis. WSM promotes cell proliferation and ALP activity when tested with bone marrow cells in concentrations between 135 and 540 μg protein/mL. The effect of WSM on ALP activity of bone marrow stromal cells is similar to that obtained by dexamethasone. These results imply that MRC-5 fibroblasts respond to differentiating factors that promote osteoblastic phenotype in bone-derived cell cultures. {\textcopyright} 2001 John Wiley \& Sons, Inc.},
	keywords = {alkaline phosphatase, Animals, article, Biomedical engineering, BMP-2, Bone, bone development, bone marrow, Bone Marrow Cells, Bone morphogenetic proteins, Cell culture, Cell Line, cell proliferation, Cells, concentration response, dexamethasone, differentiation inducing factor, dose response, Dose-Response Relationship, Drug, enzyme activity, fibroblast, Fibroblasts, Glucocorticoids, human, human cell, Humans, male, MRC-5 fibroblasts, Nacre, natural product, osteoblast, Ostreidae, oyster, phenotype, Proteins, Pulmonary diseases, Rats, Rats, Wistar, Solubility, stroma cell, time, Time Factors, Transforming Growth Factor beta, unclassified drug, Water, Water-soluble matrix},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034769134\&partnerID=40\&md5=f0ec664a074b3a67745c806152793d54},
	author = {Almeida, M.J. and Pereira, L. and Milet, C. and Haigle, J. and Barbosa, M. and Lopez, E.}
}
@article {2546,
	title = {Corrosion behaviour of titanium in biofluids containing H                    2O
                    2 studied by electrochemical impedance spectroscopy
},
	journal = {Corrosion ScienceCorros Sci},
	volume = {43},
	year = {2001},
	note = {Cited By (since 1996): 73Export Date: 21 November 2012Source: ScopusCODEN: CRRSAdoi: 10.1016/S0010-938X(00)00107-4Language of Original Document: EnglishCorrespondence Address: Fonseca, C.; Inst de Engenharia Biomedica, Porto, PortugalReferences: Hugot-Le-Goff, A., (1986) Thin Solid Films, 142, p. 193;Pascal, P., (1963) Nouveau Trait{\'e} de Chimie Min{\'e}rale, 14. , Masson \& Cie, Paris; 
Laing, P.G., Ferguson, A.B., Hodge, E.S., (1967) J. Biomed. Mater. Res., 1, p. 135; 
Dorr, L.D., Bloebaum, R., Emmanual, J., Meldrum, R., (1990) Clin. Orthop., 261, p. 82; 
Meachim, G., Williams, D.F., (1973) J. Biomed. Mat. Res., 7, p. 555; 
Sundgren, J.E., Bod{\"o}, P., Lundstr{\"o}m, I., (1986) J. Colloid Interf. Sci., 10, p. 9; 
McQueen, D., Sundgren, J.E., Ivarsson, B., Lundstr{\"o}m, I., Ekenstam, B., Svensson, A., Br{\r a}nemark, P.I., Albrektsson, T., Auger electron spectroscopic studies of titanium implants (1982) Clinical Applications of Biomaterials, p. 179. , A.J.C. Lee, T. Albrektsson, P.I. Br{\r a}nemark (Eds.), Wiley; 
Tengvall, P., Elwing, H., Sj{\"o}qvist, L., Lundstr{\"o}m, I., Bjursten, L.M., (1989) Biomaterials, 10, p. 118; 
Tengvall, P., Lundstr{\"o}m, I., (1992) Clinical Mater., 9, p. 115; 
Tengvall, P., Elwing, H., Lundstr{\"o}m, I., (1989) J. Colloid Interf. Sci., 130, p. 405; 
Sun, Z.L., Wataha, J.C., Hanks, C.T., (1997) J. Biomed. Mater. Res., 34, p. 29; 
Schepers, E., Clercq, M., Ducheyne, P., Tissue response to a bioactive glass-metal interface (1987) Implant Materials in Biofunction, p. 79. , C.D. Putter (Ed.), Elsevier, Amsterdam; 
Wang, J.Y., Tsukayama, D.T., Wicklund, B.H., Gustilo, R.B., (1996) J. Biomed. Mater. Res, 32, p. 655; 
Mu, Y., Kobayashi, T., Sumita, M., Yamamoto, A., Hanawa, T., (2000) J. Biomed. Mat. Res., 49, p. 238; 
Boukamp, B., (1989) Equivalent Circuit, User{\textquoteright}s Manual, 2nd Revised Edition, , Un. Twente Enschede, The Netherlands; 
MacDonald, J.R., Johnson, W.B., (1987) Impedance Spectroscopy, pp. 154-156. , John Wiley, New York; 
MacDonald, D.D., Urquidi-MacDonald, M., (1985) J. Electrochem. Soc., 133, p. 2018; 
Pan, J., Thierry, D., Leygraf, C., (1994) J. Biomed. Mater. Res., 28, p. 113; 
Chao, C.Y., Lin, L.F., MacDonald, D.D., (1982) J. Electrochem. Soc., 129, p. 1874; 
Armstrong, R.D., Edmondson, K., (1973) Electrochim. Acta, 18, p. 937; 
Pan, J., Thierry, D., Leygraf, C., (1996) Electrochim. Acta, 41, p. 1143; 
Cole, K.S., Cole, R.H., (1941) J. Chem. Phys., 9, p. 341; 
Rammlet, U., Reinhard, G., (1987) Corros. Sci., 27, p. 373; 
Mansfeld, F., Shih, H., Greene, H., Tsai, C.H., Analysis of EIS-data for common corrosion processes (1991) Int. Symp. on Electrochem. Imp. Analysis and Interpretation, , ASTM STP, San Diego; 
Park, J.R., MacDonald, D.D., (1983) Corros. Sci., 23, p. 295; 
Kader, J.M.E., Din, A.M.E., (1979) Br. Corros. J., 14, p. 40; 
Kader, J.M.E., Wahab, F.M.E., Shayeb, H.A.E., Khedr, M.G., (1981) Br. Corros. J., 16, p. 111; 
Hanawa, T., Ota, M., (1991) Biomaterials, 12, p. 767; 
Pan, J., Thierry, D., Leygraf, C., (1996) J. Biomed. Mater. Res., 30, p. 393; 
Hanawa, T., Ota, M., (1992) Appl. Surf. Sci., 55, p. 269; 
Le Mehaute, A., (1984) J. Stat. Phys., 36, p. 665; 
De Levie, Electrochemical response of porous and rough electrodes Advances in Electrochemistry and Electrochemical Engineering, 6. , P. Delahay (Ed.), Interscience, NY
},
	month = {2001///},
	pages = {547 - 559},
	publisher = {Elsevier Science Ltd},
	abstract = {This work aims at studying the electrochemical behaviour of titanium in the presence of an artificial biofluid containing H                        2O
                        2, mimicing the situation, where the metal is implanted in the human body and hydrogen peroxide is generated by an inflammatory reaction. A phosphate buffered saline (PBS) solution and two PBS/H
                        2O
                        2 solutions containing 50 and 150 mM of H
                        2O
                        2 were used to simulate the body fluids. The behaviour of the metal was monitored as a function of time by electrochemical impedance spectroscopy (EIS) for three weeks. After one week, the PBS/H
                        2O
                        2 solutions were replaced by fresh PBS solutions in order to simulate the end of the inflammatory process and recovery of the system. All the experiments were carried out at a constant temperature of 37 {\textdegree}C. From the simulation of the experimental EIS spectra, it was concluded that the corrosion resistance of titanium is strongly affected by the presence of H
                        2O
                        2 and when the peroxide is removed, the metal displays a sharp resistance increase. Furthermore, the oxides formed in H
                        2O
                        2 are rougher and display higher ionic conductivities than the oxides formed in the absence of peroxide. The study was complemented with potentiostatic experiments and scanning electron microscopy observation of the metal surfaces.
},
	keywords = {Biomaterials, Body fluids, Corrosion resistance, Electrochemical corrosion, electrochemical process, hydrogen peroxide, impedance technique, Implants (surgical), Phosphate buffered saline, Solutions, Spectroscopy, Titanium},
	isbn = {0010938X (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0035275589\&partnerID=40\&md5=ef23abf56839789d71056a03bc1523e3},
	author = {Fonseca, C. and Barbosa, M.A.}
}
@article {2563,
	title = {Classification of breast tissue by electrical impedance spectroscopy},
	journal = {Medical and Biological Engineering and ComputingMed. Biol. Eng. Comput.},
	volume = {38},
	year = {2000},
	note = {Cited By (since 1996): 42Export Date: 21 November 2012Source: ScopusCODEN: MBECDPubMed ID: 10829386Language of Original Document: EnglishCorrespondence Address: Marques de Sa, J.P.; INEB, Facul. Engenharia Universidade Porto, Porto, Portugal; email: jmsa@fe.op.ptReferences: Brown, B.H., Barber, D.C., Morice, A.H., Leathard, A.D., Cardiac and respiratory related electrical impedance changes in the human thorax (1994) IEEE Trans. Biomed. Eng., BME-41, pp. 729-734;Campbell, A.M., Land, D.V., Dielectric properties of female breast tissue measured in vitro at 3.2 GHz (1992) Phys. Med. Biol., 37, pp. 193-210; 
Duda, R., Hart, P., (1973) Pattern Classification and Scene Analysis, , Wiley, New York; 
Foley, D.H., Considerations of sample and feature size (1972) IEEE Trans. Inform. Theory, IT-18, pp. 618-626; 
Foster, K.R., Schwan, H.P., Dielectric properties of tissues and biological materials: A critical review (1989) CRC Crit. Reviews Biomed. Eng., 17, pp. 25-104; 
Fukunaga, K., (1972) Introduction to Statistical Pattern Recognition, , Academic Press, New York and London; 
Gersing, E., Sch{\"a}fer, M., Osypka, M., The appearance of positive phase angles in impedance measurements on extended biological subjects (1995) Innov. Techn. Biol. Med., 16 (SPEC. ISSUE 2), pp. 72-76. , Proc. {\textquoteright}CAIT Workshop on Electrode-Skin Interface in EIT{\textquoteright}; 
Heinitz, J., Minet, O., Dielectric properties of female breast tumors (1995) Proc. {\textquoteright}9th Int. Conf. Electrical Bioimpedance{\textquoteright}, pp. 356-359. , University of Heidelberg Press; 
Henderson, R.P., Webster, J.G., An impedance camera for spatially specific measurements of the thorax (1978) IEEE Trans. Biomed. Eng., BME-27, pp. 250-254; 
Jossinet, J., Variability of impedivity in normal and pathological breast tissue (1996) Med. Biol. Eng. Comput., 34, pp. 346-350; 
Jossinet, J., The impedivity of freshly excised human breast tissue (1998) Physiol. Meas., 19, pp. 61-75; 
Jossinet, J., Lavandier, B., The discrimination of excised cancerous breast tissue samples using impedance spectroscopy (1998) Bioelectrochem. Bioenerg., 45, pp. 161-167; 
Kubicek, W.G., Patterson, R.P., Witsoe, D.A., Impedance cardiography as a noninvasive method of monitoring cardiac function and other parameters of the cardiovascular system (1970) Ann. N.Y. Acad. Sciences, 170, pp. 24-732; 
Morimoto, T., Kinouchi, Y., Iritani, T., Kimura, S., Konishi, Y., Mitsuyama, N., Komaki, K., Monden, Y., Measurement of the electrical bio-impedance of breast tumors (1990) Eur. Surg. Res., 22, pp. 86-92; 
Raudys, S., Pikelis, V., On dimensionality, sample size, classification error and complexity of classification algorithm in pattern recognition (1980) IEEE Trans. Patt. Anal. Mach. Intel., PAMI-2, pp. 242-252; 
Shapiro, S.S., Wilk, S.S., Chen, S.W., A comparative study of various tests for normality (1968) J. Am. Stat. Ass., 63, pp. 1343-1372; 
Swain, P.H., The decision tree classifier: Design and potential (1977) IEEE Trans. Geosci. Electronics, GE-15, pp. 142-147; 
Schwan, H.P., Alternating current spectroscopy of biological substances (1959) Proc. IRE, 47, pp. 1841-1855; 
Surowiec, A.J., Stuchly, S.S., Barr, J.R., Swarup, A., Dielectric properties of breast carcinoma and the surrounding tissues (1988) IEEE Trans. Biomed. Eng., BME-35, pp. 257-263; 
Tachibana, S., Aguilar, J.A., Birzis, L., Scanning the interior of living brain by impedography (1970) J. of App. Physiol., 28, pp. 534-539
},
	month = {2000///},
	pages = {26 - 30},
	publisher = {Peter Peregrinus Ltd},
	abstract = {Electrical impedance spectroscopy is a minimally invasive technique that has clear advantages for living tissue characterisation owing to its low cost and ease of use. The present paper describes how this technique can be applied to breast tissue classification and breast cancer detection. Statistical analysis is used to derive a set of rules based on features extracted from the graphical representation of electrical impedance spectra. These rules are used hierarchically to discriminate several classes of breast tissue. Results of statistical classification obtained from a data set of 106 cases representing six classes of excised breast tissue show an overall classification efficiency of ~92\% with carcinoma discrimination > 86\%.Electrical impedance spectroscopy is a minimally invasive technique that has clear advantages for living tissue characterization owing to its low cost and ease of use. The present paper describes how this technique can be applied to breast tissue classification and breast cancer detection. Statistical analysis is used to derive a set of rules based on features extracted from the graphical representation of electrical impedance spectra. These rules are used hierarchically to discriminate several classes of breast tissue. Results of statistical classification obtained from a data set of 106 cases representing six classes of excised breast tissue show an overall classification efficiency of approximately 92\% with carcinoma discrimination >86\%.
},
	keywords = {article, Breast, breast cancer, Breast Neoplasms, cancer classification, Data classification, Discriminant Analysis, Electric Impedance, Electrical impedance spectroscopy, Electrodiagnosis, Feature extraction, Female, human, Humans, impedance, Impedance spectroscopy, major clinical study, Medical imaging, Signal Processing, Computer-Assisted, Spectroscopy, Statistical analysis, Statistical methods, Tissue, Tissue characterisation},
	isbn = {01400118 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033962139\&partnerID=40\&md5=1510d493688d338d985aad2a31d1caeb},
	author = {Estrela Da Silva, J. and Marques De S{\'a}, J.P. and Jossinet, J.}
}
@conference {2555,
	title = {A computational model of visual cortex receptive fields using spatio-temporal filters},
	booktitle = {Proceedings of the IEEE/EMBS Region 8 International Conference on Information Technology Applications in Biomedicine, ITABProc IEEE EMBS Reg 8 Int Conf Inf Technol Appl Biomed ITAB},
	series = {ITAB-ITIS 2000},
	year = {2000},
	note = {Conference code: 58015Cited By (since 1996): 1Export Date: 21 November 2012Source: ScopusLanguage of Original Document: EnglishCorrespondence Address: Milanova, M.G.; INEB, Inst. of Biomedical Engineering, University of Porto, Pra{\c c}a Coronel Pacheco, 1 4050 Porto, Portugal; email: milanova@ineb.fe.up.ptReferences: Hubel, D.H., Wiesel, T.N., Receptive fields, binocular interaction, and functional architecture in the cat{\textquoteright}s visual cortex (1962) Journal of Physiology (London), 160, pp. 106-154;DeAngelis, G.C., Ohzawa, I., Freeman, R.D., Receptive-field dynamic in the central visual pathways (1995) Trends in Neuroscience, 18, pp. 451-458; 
Atick, J., Redlich, N., What does the retina know about natural scenes? (1992) Neural Computation, 4, pp. 196-210; 
Barlow, H.B., What is the computational goal of the neocortex? (1994) Large-Scale Neuronal Theories of the Brain, pp. 1-22. , C. Koch and J. L. Davis, eds, Cambridge, MA, MIT Press; 
Olshausen, B.A., Field, D.J., Emergence of simple-cell receptive field properties by learning a sparse code for natural images (1996) Nature, 381, pp. 607-609; 
Rao, R.P.N., Ballard, D.H., Dynamic model of visual recognition predicts neural response properties in the visual cortex (1997) Neural Computation, 9, pp. 721-763; 
Olshausen, B.A., (2000) Sparse coding of time-varying natural images, , http://psyweb.ucdavis.edu/olshausen; 
Oja, E.E., Neural networks, principal components and subspaces (1989) International Journal of Neural Systems, 1, pp. 61-68; 
Field, D.J., What is the goal of sensory coding? (1994) Neural Computation, 6, pp. 559-601; 
Field, D.J., Relations between the statistics of natural images and the response properties of cortical cells (1987) J. Opt. Soc. Am.A, 4, pp. 2379-2394; 
Bell, A.J., Sejnowski, T.J., The independent components of natural scenes are edge filters (1997) Vision Research, 37, pp. 3327-3338; 
Smith, A.T., Snowdon, R.S., (1994) Visual Detection of Motion, , Academic Press Inc., San Diego; 
Zemel, R.S., Sejnowski, T.J., A model for encoding multiple object motions and self-motion in area MST of primate visual cortex (1998) Journal of Neuroscience, 18, pp. 531-547; 
Van Hateren, J.H., Ruderman, D.L., Independent component analysis of natural image sequences yields spatio-temporal filters similar to simple cells in primary visual cortex (1998) Proc. Royal Soc. London B, 265, pp. 2315-2320; 
Jones, J.P., Palmer, L.A., The two-dimensional spatial structure of simple receptive fields in cat striate cortex (1987) Journal of Neurophysiology, 58, pp. 1187-1211; 
Jones, J.P., Palmer, L.A., An Evaluation of the two-dimensional Gabor filter model of simple receptive fields in cat striate cortex (1987) Journal of Neurophysiology, 58, pp. 1233-1258; 
Jones, J.P., Stepnoski, A., Palmer, L.A., The two-dimensional spectral structure of simple receptive fields in cat striate cortex (1987) Journal of Neurophysiology, 58, pp. 1212-1232; 
Lewicki, M.S., Olshausen, B.A., Probabilistic framework for the adaptation and comparison of image codes (1999) Journal of the Optical Society of America, 16, pp. 1587-1601; 
Chua, L.O., Yang, L.L., Cellular Neural Networks: Theory and Applications (1988) IEEE Trans. on Circuits and Systems, 35, pp. 1257-1290
Sponsors: IEEE},
	month = {2000///},
	pages = {129 - 134},
	address = {Arlington, VA},
	abstract = {It is known that neurons in the primary visual cortex are characterized by receptive fields that are selective for specific spatial orientations. It is believed that the sense of object motion and velocity are also related to these fields, as objects in natural scenes are represented by a sparse set of statistically independent components, such as edges. Currently, computational models of receptive fields consider only spatial components, and thus cannot account for time-varying sensory stimuli. In this paper, a model based on independent components analysis and cellular neural networks is proposed. This approach extends existing models to incorporate temporal aspects of sequences of images of natural scenes.},
	keywords = {Cellular neural networks, Computational methods, ICA, Independent component analysis, Mathematical models, Neurology, Receptive fields, Receptive fields (RF), Spatial filters, Spatio-temporal filters, Visual modeling},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0034451070\&partnerID=40\&md5=f1377b4b2f2403be91a21d0feb639fb7},
	author = {Milanova, M.G. and Elmaghraby, A.S. and Wachowiak, M.P. and Campilho, A.}
}
@article {2553,
	title = {Concept, design and fabrication of smart orthopedic implants},
	journal = {Medical Engineering and PhysicsMed. Eng. Phys.},
	volume = {22},
	year = {2000},
	note = {Cited By (since 1996): 29Export Date: 21 November 2012Source: ScopusCODEN: MEPHEdoi: 10.1016/S1350-4533(00)00062-XPubMed ID: 11165144Language of Original Document: EnglishCorrespondence Address: Burny, F.; Service d{\textquoteright}Orthopedie-Traumatologie, Cliniques Universitaires Bruxelles, H{\^o}pital Erasme, Brussels, Belgium; email: fburny@ulb.ac.beChemicals/CAS: Biocompatible Materials; Ceramics; Metals; Stainless Steel, 12597-68-1References: Burny, F., Donkerwolcke, M., Moulart, F., Clinical application of the monitoring of orthopedic implants (1993), pp. 138-146. , Burny R, Puers F, editors. Monitoring of orthopedic implants. A biomaterials microelectronics - challenge. Amsterdam: ElsevierBurny, F., Zucman, J., Bourgois, R., Aubriot, J.H., Sedel, J., Utilisation des jauges extensom{\'e}triques pour la mesure de la consolidation des fractures du tibia trait{\'e}es par enclouage centrom{\'e}dullaire. Etude th{\'e}orique et premiers r{\'e}sultats (1971) Acta Orthop Belg, 37, pp. 266-277;Moulart, F., (1975) R{\'e}{\'e}ducation des fractures hautes du f{\'e}mur. Mesure des d{\'e}formations du mat{\'e}riel d{\textquoteright}ost{\'e}osynthese, , M{\'e}moire de Licence en Kin{\'e}sith{\'e}rapie, Universit{\'e} Libre de Bruxelles; 
Burny, F., Moulart, F., Hinsenkamp, M., Traitement des fractures m{\'e}tastatiques de la region sous-trochant{\'e}rienne. Etude biom{\'e}canique; cas cliniques (1976) Acta Orthop Belg, 42, pp. 31-41; 
Burny, F., Donkerwolcke, M., Telemetry in the musculoskeletal system (1991), pp. 391-394. , Payne PA, editor. Concise encyclopedia of biological and biomedical measurement systems. Oxford: Pergamon PressPuers, R., Burny, F., Biotelemetry in orthopaedics (1992), 3, pp. 285-306. , Muster D. et al., editors. European Materials Research Society Monographs. Biomaterials. Hard Tissue Repair and Replacement, Amsterdam: ElsevierKadefors, R., Kaiser, E., Petersen, I., Energizing implantable transmitters by means of coupled inductance coils (1969) IEEE Trans Biomed Eng, 16 BME, pp. 177-183; 
Ko, W.H., Liang, S.P., Fung, C.D.F., Design of radio-frequency powered coils for implant intruments (1977) Med Biol Eng Comput, 15, pp. 634-640; 
Donaldson, N., De, N., Perkins, T.A., Analysis of resonant coupled coils in the design of radio frequency trancutaneous links (1983) Med Biol Eng Comput, 21, pp. 612-627; 
Grossman, D.G., Machining a machinable glass-ceramic (1978) Vacuum, 28, pp. 55-61; 
Van Schuylenbergh, K., Puers, R., The influence of moisture leakage on implant strain measurements with resistive strain gauges (1993), pp. 125-137. , Burny R, Puers F, editors. Monitoring of orthopedic implants. A biomaterials microelectronics - challenge. Amsterdam: ElsevierWrangl{\'e}n, G., (1985) An introduction to corrosion and protection of metals, , London: Chapman \& Hall; 
Barbosa, M.A., Surface layers and the reactivity of metallic implants (1992), pp. 257-284. , Muster D, editor. Biomaterials - hard tissue repair and replacement, E-MRS Monographs. North-Holland: Elsevier ScienceNicholas, M.G., Active metal brazing (1986) British Ceramic Transactions Journal, 85, pp. 144-146; 
Pattee, H.E., Joining ceramics to metals and other materials (1972) Welding Research Council Bulletin, 178, pp. 2435-2442. , New York; 
Nicholas, M.G., Crispin, R.M., Brazing ceramics with alloys containing titanium (1989) Ceramic Engineering and Science Proceedings, 10, pp. 1602-1612; 
Rodes, F., Zardini, C., Devall, Y., Puers, R., Van Schuylenbergh, K., Burny, F., Donkerwolcke, M., Moulart, F., Direct digitization of low level transducer outputs for low power intracorporal telemetry systems (1993), pp. 401-409. , Mancini P, Fioretti S, editors. 12th Int. Symposium on Biotelemetry, Ancona. Pisa, Italy: Universitaria Litografia FeliciFlack, F.C., James, E.D., Schlapp, D.M., Mutual inductance of air-cored coils: Effect on design of radio-frequency coupled implants (1971) Med Biol Eng Comput, 9, pp. 79-85; 
},
	month = {2000///},
	pages = {469 - 479},
	abstract = {Extensive clinical experience has been built up using orthopaedic implants instrumented with strain gauges connected to a Wheatstone bridge by means of percutaneous leads. This research showed the medical relevance of the monitoring of the deformation of implants as a powerful tool to evaluate nursing and rehabilitation exercises, for tracing dangerous overloads and anticipating implant failure and also to observe the healing process. The IMPACT 3500 project focuses on the instrumentation of femoral implants with on board sensors: regular Benoist-Girard implants have been modified, to contain a {\textquoteright}sensing cell{\textquoteright}, and thoroughly tested in vitro and in vivo. The implant deformations are measured with resistive strain gauges, and the signal is transferred to a personal computer for processing and display, via a hard wired connection, or via a telemetry system. Two fully implantable wireless designs, called Linkstrain and Sealstrain, are powered from the outside by magnetic induction. As Sealstrain contains the whole telemetric system in its cavity, the highest miniaturization was required; this seriously deteriorates the efficiency of the inductive power link. {\textcopyright} 2001 IPEM.},
	keywords = {article, atomic absorption spectrometry, Biocompatible Materials, biomechanics, body movement, Bone Plates, ceramics, compression, Corrosion, Failure of implants, Femoral Fractures, femur, Fracture healing, healing, Humans, materials testing, metal, metal implantation, Metals, microcomputer, Microscopy, Electron, Scanning, Monitoring, Physiologic, Orthopedic implants, orthopedic surgery, priority journal, Prostheses and Implants, Prosthesis Design, prosthesis failure, Rehabilitation, Scanning electron microscopy, sensor, Sensors, Smart implants, Spectrum Analysis, Stainless steel, strain gauge transducer, Strain measurements, Stress, Mechanical, Telemetry, Weight-Bearing},
	isbn = {13504533 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-20244372369\&partnerID=40\&md5=456fea60c077d9bd4569169438349a07},
	author = {Burny, F. and Donkerwolcke, M. and Moulart, F. and Bourgois, R. and Puers, R. and Van Schuylenbergh, K. and Barbosa, M. and Paiva, O. and Rodes, F. and B{\'e}gueret, J.B. and Lawes, P.}
}
@article {2557,
	title = {Crystallization and microstructure analysis of calcium phosphate-based glass ceramics for biomedical applications},
	journal = {Journal of Non-Crystalline SolidsJ Non Cryst Solids},
	volume = {272},
	year = {2000},
	note = {Cited By (since 1996): 42Export Date: 21 November 2012Source: ScopusCODEN: JNCSBdoi: 10.1016/S0022-3093(00)00115-0Language of Original Document: EnglishCorrespondence Address: Zhang, Yong; Inst de Engenharia Biomedica, Porto, PortugalReferences: Kitsugi, T., Yamamuro, T., Nakamura, T., Kotani, S., Kokubo, T., Takeuchi, H., (1993) Biomaterials, 14, p. 216;Kitsugi, T., Yamamuro, T., Nakamura, T., Oka, M., (1995) Biomaterials, 16, p. 1101; 
Doremus, R.H., (1992) J. Mater. Sci., 27, p. 285; 
Kokubo, T., (1993) An Introduction to Bioceramics, p. 75. , in L.L. Hench Wilson J. (Eds.), World Scientific, Singapore; 
Tracy, B., Doremus, R.H., (1984) J. Biomed. Mater. Res., 18, p. 719; 
Jha, L.J., Best, S.M., Santos, J.D., Bonfield, W., (1997) J. Mater. Sci.: Mater. Med., 8, p. 185; 
Hench, L.L., (1991) J. Am. Ceram. Soc., 74, p. 1487; 
Hench, L.L., Anderson, {\"O}., (1993) An Introduction to Bioceramics, p. 41. , in: L.L. Hench, J. Wilson (Eds.), World Scientific, Singapore; 
Nakamura, T., Yamamuro, T., Higashi, S., Kokubo, T., Ito, S., (1985) J. Biomed. Mater. Res., 19, p. 685; 
H{\"o}land, W., Vogel, W., Nauman, K., Gummel, J., (1985) J. Biomed. Mater. Res., 19, p. 303; 
Kasuga, T., Abe, Y., (1998) J. Mater. Res., 13, p. 1; 
Rawson, H., (1960) Inorganic Glass-Forming System, p. 155. , Academic, London; 
Abe, Y., Naruse, A., Hosoe, M., Fukui, H., Hasegawa, J., (1975) In: The 1975 Symposium on Biomaterials, p. 202. , Kyoto; 
Abe, Y., (1990) In: International Forum on Fine Ceramics {\textquoteright}90, p. 78. , Nagoya; 
Kasuga, T., Abe, Y., (1999) J. Non-Cryst. Solids, 243, p. 70; 
Hosono, H., Zhang, Z., Abe, Y., (1989) J. Am. Ceram. Soc., 72, p. 1587; 
Posner, A.S., (1969) Phys. Rev., 49, p. 760; 
Kitsugi, T., Yamamuro, T., Nakamura, T., Kotani, S., Kokubo, T., Takeuchi, H., (1993) Biomaterials, 14, p. 216; 
Klein, C.P., De Blieck-Hogervorst, J.M., Wolke, J.G., De Groot, K., (1990) Biomaterials, 11, p. 509; 
Kitsugi, T., Yamamuro, T., Nakamura, T., Oka, M., (1995) Biomaterials, 16, p. 1101; 
M{\"u}ller-Mai, C., Berger, G., Voigt, C., Bakki, B., Gross, U., (1997) Bioceramics, 10, p. 53. , in: L. Sedel, C. Rey (Eds.), Elsevier, Amsterdam; 
Vogel, J., Schulze, K.-J., Reif, D., Hartmann, P., Platzbecker, U., Leuner, B., (1997) Bioceramics, 10, p. 57. , in: L. Sedel, C. Rey (Eds.), Elsevier, Amsterdam; 
Gil, F.J., Terradas, R., Clement, J., Avila, G., Martinez, S., Planell, J.A., (1997) Bioceramics, 10, p. 553. , in: L. Sedel, C. Rey (Eds.), Elsevier, Amsterdam; 
Sun, K.-H., (1947) J. Am. Ceram. Soc., 30, p. 277; 
Arm, D.M., Tencer, A.F., Brain, S.D., Celino, D., (1996) Biomaterials, 17, p. 703; 
Denissen, D., Van Beck, E., Lowick, C., Papapoulos, S., Van Den Hoof, A., (1993) Bone Miner., 25, p. 123; 
Nguyen, T.H., Cromwell, M., Wonc, R., Beck, L.S., Plouhar, P., Onpipattatanskul, B., (1994) Proceedings of the International Symposium Control. Rel. Bioact. Mater., 21, p. 122; 
Uchida, A., Shinto, Y., Araki, N., Ono, K., (1992) J. Orthop. Res., 10, p. 440
},
	month = {2000///},
	pages = {14 - 21},
	publisher = {Elsevier Science Publishers B.V.},
	abstract = {Calcium phosphate glasses and glass ceramics (CaO/P                        2O
                        5 = 1.25 in molar ratio) modified by small amounts of additives such as Na
                        2O, MgO, TiO
                        2 and ZrO
                        2 were prepared after appropriate heat treatment for nucleation and crystallization. Based upon differential thermal analysis (DTA) results, a two-step heat treatment was used for crystal nucleation and growth. X-ray diffraction (XRD) analysis demonstrated that bioresorbable β-Ca
                        2P
                        2O
                        7 (β-DCP), β-Na
                        2CaP
                        2O
                        7, Na
                        2Mg(PO
                        3)
                        4 phases were formed in the glass matrix depending upon the relative contents of the additives. By adding higher contents of Na
                        2O and TiO
                        2 and using CaO/P
                        2O
                        5 = 1.5-2.0, crystallization of β-DCP and Ca
                        3(PO
                        4)
                        2 (β-TCP), and the formation of a dense structure in the glass ceramics were obtained. The precipitation of these crystals could be well distinguished at a magnification of 4000{\texttimes} and phases were dispersed in areas of micron size. A porous structure may be easily formed after the soluble phases are dissolved in physiological media. These glass ceramics with high CaO/P
                        2O
                        5 ratio, modified by the above mentioned additives are expected to find use as implants for bone replacement/regeneration and drug delivery carriers synergistically, because the soluble phases may act as drug delivery carriers and the porous structure will allow for bone ingrowth.
},
	keywords = {Additives, Biomaterials, Calcium Compounds, calcium phosphate, Ceramic materials, Crystal microstructure, Crystallization, Differential thermal analysis, glass, Glass ceramics, Nucleation, Phosphates, Porous materials, Precipitation (chemical), Sodium dioxide, X ray crystallography},
	isbn = {00223093 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033706457\&partnerID=40\&md5=5fc6504dbcb6390e2909c05594ba9110},
	author = {Zhang, Y. and Santos, J.D.}
}
@article {2574,
	title = {CaO-P                    2O
                    5 glass hydroxyapatite double-layer plasma-sprayed coating: In vitro bioactivity evaluation
},
	journal = {Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res.},
	volume = {45},
	year = {1999},
	note = {Cited By (since 1996): 31Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(19990615)45:4<376::AID-JBM13>3.0.CO;2-SLanguage of Original Document: EnglishCorrespondence Address: Santos, J.D.; Departamento Engenharia Metalurgica, FEUP, Universidade do Porto, R. dos Bragas, 4099 Porto Codex, PortugalChemicals/CAS: Alloys; Calcium, 7440-70-2; CaO-SiO2-P2O5-CaF2 glass; Coated Materials, Biocompatible; Durapatite, 1306-06-5; Phosphates; Powders; Resin Cements; Solutions; titanium alloy (TiAl6V4), 12743-70-3; Titanium, 7440-32-6References: Denissen, H., Mangano, C., Venini, G., (1985) Hydroxylapatite Implants., , Padua: Piccin Nuova Libraria, S.P.A;Van Raemdonck, W., Ducheyne, P., De Meester, P., Calcium phosphate ceramics (1984) Metal and Ceramic Biomaterials, pp. 143-166. , Ducheyne P, Hastings GW, editors, Boca Raton, FL: CRC Press; 
Legeros, R.Z., Incorporation of magnesium in synthetic and biological apatites (1994) Tooth Enamel IV., pp. 32-36. , Fearnead RW, editor. Amsterdam: Elsevier Science; 
Jha, L.J., Best, S.M., Knowles, J.C., Rehman, I., Santos, J.D., Bonfield, W., Preparation and characterization of fluoride-substituted apatites (1997) J Mater Sci Mater Med, 8, pp. 185-191; 
Geesink, R.G.T., Degroot, K., Chemical implant fixation using hydroxyl-apatite coatings (1987) Clin Orthop, 2, pp. 147-170; 
Radin, S., Ducheyne, P., The effect of plasma sprayed induced changes in the characteristics on the in vitro stability of calcium phosphate ceramics (1990) Transactions of the 16th Annual Meeting of the Society for Biomaterials XIII, pp. 128-130; 
Cook, S.D., Thomas, K.A., Hydroxyapatite coated porous titanium for use as an orthopaedic biologic attachment (1989) Clin Orthop, 230, pp. 303-312; 
McPherson, R., Gane, N., Bastow, T.J., Structural characterization of plasma-sprayed hydroxylapatite coatings (1995) J Mater Sci Mater Med, 6, pp. 327-334; 
De Groot, K., Geesink, R., Klein, C.P.A.T., Serekian, P., Plasma sprayed coatings of hydroxylapatite (1987) J Biomed Mater Res, 21, pp. 1375-1381; 
Ravagliolli, A., Krajewszki, A., Laudadio, P., Presutti, L., Cunsolo, E., Martinetti, R., About ceramics for stapedial prostheses and the ossicular chain (1992) Fifth International Symposium on Ceramics in Medicine-Bioceramics 5, pp. 451-458. , Yamamuro T, Kokubo T, Nakamura T, editors. Kyoto: Kobunshi Kankokai; 
Hastings, G.W., Daily, D., Hydroxyapatite coatings (1989) First International Symposium on Ceramics in Medicine-Bioceramics 1, pp. 355-358. , Oonishi H, Aoki H, Sawai K, editors. Tokyo: Ishiyaku EuroAmerica; 
Aoki, H., Medical applications of hydroxyapatite (1991) Science and Medical Applications of Hydroxyaptite, pp. 175-192. , Aoki H, Sawai K, editors. Tokyo: Takyama Press System Centre; 
Rey, C., Calcium phosphate biomaterials and bone mineral: Differences in composition, structures and properties (1990) Biomaterials, 11, pp. 13-15; 
Evans, G., Behiri, J., Currey J, B.W., Microhardness and young{\textquoteright}s modulus in cortical bone exhibiting a wide range of mineral volume fractions, and in a bone analogue (1990) J Mater Sci Mater Med, 1, pp. 38-43; 
Rey, C., Froche, M., Heughebaert, M., Heughebaert, J.C., Lacout, J.L., Vignoles, M., Apatite chemistry in biomaterial preparation, shaping and biological behaviour (1991) Fourth International Symposium on Ceramics in Medicine-Bioceramics 4, pp. 57-60. , Bonfield W, Hastings GW, Tanner KE, editors. London: Butterworth-Heinemann; 
Knowles, J.C., Talal, S., Santos, J.D., Sintering effects in a glass reinforced hydroxyapatite (1996) Biomaterials, 17, pp. 1437-1442; 
Kokubo, T., Hayashi, T., Sakka, S., Kitsugi, T., Yamamuro, T., Takagi, M., Shibuya, T., Surface structure of a load bearable bioactive glass-ceramic A-W (1981) High Tech Ceramics, pp. 175-184. , Vicenzini P, editor. Amsterdam: Elsevier Science Publisher BV; 
Hench, L., Anderson, O.H., LaTorre, G.P., The kinetics of bioactive ceramics. Part III: Surface reactions for bioactive glasses compared with inactive glass (1991) Fourth International Symposium on Ceramics in Medicine-Bioceramics 4, , Bonfield W, Hastings GW, Tanner KE, editors. London: Butterworth-Heinemann; 155-162; 
Daculsi, G., LeGeros, R.Z., Heughebart, M., Barbieux, I., Formation of a carbonate apatite crystals after implantation of calcium phosphate ceramics (1990) Calcif Tissue Int, 46, pp. 20-27; 
Kokubo, T., Ito, S., Huang, Z.T., Hayashi, T., Sakka, S., Kitsugi, T., Yamamuro, T., Ca-P rich layer formed on high strength bioactive glass-ceramic A-W (1990) J Biomed Mater Res, 24, pp. 331-343; 
Ducheyne, P., Radin, S., Ishikawa, K., Kim, C.S., Dissolution and precipitation of calcium phosphate phases on biomaterials correlates with in vivo bioactivity (1991) Fourth International Symposium on Ceramics in Medicine-Bioceramics 4, pp. 135-144. , Bonfield W, Hastings GW, Tanner KE, editors. London: Butterworth-Heinemann; 
Rey, C., Hina, A., Amrah-Bouali, S., Ranz, X., Surface reactions of calcium-phosphate bioceramics, comparison with bone mineral surface chemistry (1995) Fourth Euro Ceramics., 8, pp. 301-312. , Ravaglioli A, editor. Faenza: Gruppo Editoriale Faenza Editrice; 
Van Blitterswijk, C.A., Grote, J.J., Kuypers, W., Blok-Van Hoek, C.J.G., Daems, W.T., Bioreactions at the tissue/hydroxyapatite interface (1985) Biomaterials, 6, pp. 243-251; 
Ducheyne, P., Bioceramics: Material characteristics versus in vivo behavior (1987) J Biomed Mater Res, 21, pp. 219-236; 
Hyakuna, K., Yamamuro, T., Kotoura, Y., Oka, M., Nakamura, T., Kitsogi, T., Kokubo, T., Kushitani, H., Surface reactions of calcium phosphate ceramics to various solutions (1990) J Biomed Mater Res, 24, pp. 471-488; 
Santos, J.D., Reis, R.L., Monteiro, F.J., Knowles, J.C., Hastings, G.W., Liquid phase sintering of hydroxyapatite by phosphate and silicate glass additions: Structure and properties of the composites (1995) J Mater Sci Mater Med, 6, pp. 348-352; 
Tanahashi, M., Yao, T., Kokubo, T., Minoda, M., Miyamoto, T., Nakamura, T., Yamamuro, T., Apatite coated on organic polymers by biomimetic process: Improvement in adhesion to substrate by HC1 treatment (1995) J Mater Sci Mater Med, 6, pp. 319-326; 
Neo, M., Nakamura, T., Yamamuro, T., Ohtsuki, C., Kokubo, T., Bando, Y., A comparative study of ultrastructures of the interfaces between four kinds of surface-active ceramic and bone (1992) J Biomed Mater Res, 26, pp. 1419-1432; 
Li, P., Ohtsuki, C., Kokubo, T., Nakanishi, K., Soga, N., Nakamura, T., Yamamuro, T., Process of formation of bone-like apatite layer on silica gel (1993) J Mater Sci Mater Med, 4, pp. 127-131; 
Santos, J.D., Jha, L.J., Monteiro, F.J., Surface modifications of glass-reinforced hydroxyapatite composites (1995) Biomaterials, 16, pp. 521-526; 
Neo, M., Kotani, S., Fujita, Y., Yamamuro, T., Bando, Y., Ohtsuki, C., Kokubo, T., Differences in ceramic-bone interface between surface-active ceramics and resorbable ceramics: A study by scanning and transmission electron microscope (1992) J Biomed Mater Res, 26, pp. 255-267; 
Li, P., Yang, Q., Zhang, F., Kokubo, T., The effect of residual glassy phase in a bioactive glass-ceramic on the formation of its surface apatite layer in vitro (1992) J Mater Sci Mater Med, 3, pp. 452-456; 
Kotani, S., Fujita, Y., Kitsugi, T., Nakamura, T., Yamamuro, T., Bone bonding mechanism of β-tricalcium phosphate (1991) J Biomed Mater Res, 25, pp. 1303-1315; 
Daculsi, G., LeGeros Rz, H.M., Barbieux, I., Formation of carbonate-apatite crystals after implantation of calcium phosphate ceramics (1990) Calcif Tissue Int, 46, pp. 20-27; 
Silva, P.L., Monteiro, F.J., Santos, J.D., Knowles, J.C., Adhesion and microstructural characterisation of plasma sprayed hydroxyapatite-glass ceramic coatings onto Ti-6Al-4V substrates (1998) Surf Coatings Technol, 102, pp. 191-196; 
Kokubo, T., Kushitani, H., Ohtsuki, C., Sakka, S., Yamamuro, T., Chemical reaction of bioactive glass and glass-ceramics with a simulated body fluid (1992) J Biomed Mater Res, 3, pp. 79-83; 
Blakeslee, D.M., Dal-Favero, M.E., (1997) NIST X-ray Photoelectron Spectroscopy Database, , Gaitherburg, MD: National Institute of Standards and Technology; 
Anderson, O.H., Kangasniemi, I., Calcium phosphate formation at the surface of bioactive glass in vitro (1991) J Biomed Mater Res, 25, pp. 1019-1030; 
Santos, J.D., Jha, L.J., Monteiro, F.J., In vitro calcium phosphate formation on SiO 2-Na 2O-CaO-P 2O 5 glass reinforced hydroxyapatite composite: A study by XPS analysis (1996) J Mater Sci Mater Med, 7, pp. 181-185; 
Verhoeven, J.A.T., Van Doveren, H., An XPS investigation of the interface of methane, ethyne, ethene and ethane with barium surface (1982) Surf Sci, 123, pp. 369-383
},
	month = {1999///},
	pages = {376 - 383},
	publisher = {John Wiley \& Sons Inc},
	abstract = {Double-layer composite coatings composed of a P                        2O
                        5-based glass/Ca
                        10(PO
                        4)
                        6(OH)
                        2 (HA) mixture top layer and a simple HA underlayer, on Ti-6AI-4V substrates, were prepared using a plasma-spraying technique. The in vitro bioactivity of these coatings was assessed by immersion testing in simulated body fluid. Both scanning electron microscopy (SEM) analysis and the ionic solution changes followed by atomic absorption spectroscopy and the molybdenum blue method demonstrated that these composite coatings induce a faster surface Ca-P layer formation than the simple HA coatings used as a control. X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the Ca-P layer formed was apatite. The combination of SEM and XPS analyses showed that the apatite layer was a calcium-deficient hydroxyapatite with a Ca/P ranging from 1.3 to 1.4 with CO
                        3
                        2- groups contained in the structure.
Double-layer composite coatings composed of a P
                        2O
                        5-based glass/Ca
                        10(PO
                        4)
                        6(OH)
                        2 (HA) mixture top layer and a simple HA underlayer, on Ti-6AI-4V substrates, were prepared using a plasma-spraying technique. The in vitro bioactivity of these coatings was assessed by immersion testing in simulated body fluid. Both scanning electron microscopy (SEM) analysis and the ionic solution changes followed by atomic absorption spectroscopy and the molybdenum blue method demonstrated that these composite coatings induce a faster surface Ca-P layer formation than the simple HA coatings used as a control. X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the Ca-P layer formed was apatite. The combination of SEM and XPS analyses showed that the apatite layer was a calcium-deficient hydroxyapatite with a Ca/P ranging from 1.3 to 1.4 with CO
                        3
                        2- groups contained in the structure.
},
	keywords = {alloy, Alloys, Apatite, article, Atomic absorption spectroscopy, Atomic spectroscopy, Bioactivity, Biocompatibility, biomaterial, Biomaterials, Biomedical engineering, Body fluids, Calcium, Calcium Compounds, CaO SiO2 P2O5 CaF2 glass, CaO-SiO2-P2O5-CaF2 glass, Chemical analysis, chemical composition, Coated Materials, Biocompatible, Double layer coatings, Double-layer coatings, Durapatite, film, glass, Glass plasma spraying, human, Humans, hydroxyapatite, immersion, implant, in vitro study, Ionic solution changes, Magnesium, materials testing, Molybdenum blue, Multilayers, Particle Size, phosphate, Phosphate minerals, Phosphates, Phosphorus Compounds, phosphorus pentoxide, Plasma spraying, potassium, powder, Powders, resin cement, Resin Cements, Scanning electron microscopy, Sodium, solution and solubility, Solutions, structure analysis, Substrates, Surface Properties, surface property, thickness, Tissue, Titanium, titanium alloy (TiAl6V4), Titanium alloys, X ray photoelectron spectroscopy},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0033563315\&partnerID=40\&md5=8c0de1e25709302641b203efac50337b},
	author = {Ferraz, M.P. and Monteiro, F.J. and Santos, J.D.}
}
@article {2584,
	title = {Chromium determination in osteoblast-like cell culture medium by catalytic cathodic stripping voltammetry with a mercury microelectrode},
	journal = {Journal of Trace Elements in Medicine and BiologyJ. Trace Elem. Med. Biol.},
	volume = {12},
	year = {1998},
	note = {Cited By (since 1996): 7Export Date: 21 November 2012Source: ScopusCODEN: JTEBFPubMed ID: 9760419Language of Original Document: EnglishCorrespondence Address: Morais, S.; Inst. Engenharia Biomedica (INEB), Praca Coronel Pacheo 1, 4050 Porto, PortugalChemicals/CAS: Acetates; Chromium, 7440-47-3; Culture Media; Mercury, 7439-97-6; NitratesReferences: Ferguson Jr., A.B., Laing, P.G., Hodge, E.S., The ionization of metal implants in living tissues (1960) J. Bone Joint Surg., 42 A, pp. 77-90;Ferguson Jr., A.B., Akahoshi, Y., Laing, P.G., Hodge, E.S., Trace metal ion concentration in the liver, kidney, spleen and lung of normal rabbits (1962) J Bone Joint Surg., 44 A, pp. 317-322; 
Ferguson Jr., A.B., Akahoshi, Y., Laing, P.G., Hodge, E.S., Characteristics of trace ions released from embedded metal implants in the rabbit (1962) J. Bone Joint Surg. 1962, 44 A, pp. 323-336; 
Lux, F., Ziesler, R., Investigations of the corrosive deposition of components of metal implants and of the behaviour of biological trace elements in metallosis tissue by means of instrumental multi-elemental activation analysis (1974) J. Radioanal. Chem., 19, pp. 289-297; 
Smith, G.K., Black, J., Estimation of in vivo type 316L stainless steel corrosion rate from blood transport and organ accumulation data (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 223-247. , ASTM STP 859 (Fraker, A. C. and Griffin, C. D. ,Eds.). American Society for Testing and Materials, Philadelphia; 
Minski, M.J., Dobbs, H.S., (1980) Trace Element Analytical Chemistry in Medicine and Biology, pp. 339-350. , Bratter, P., Schramel, P., Eds., Walter de Gruyter, Berlin; 
Smith, G.K., (1982) Systemic Transport and Distribution of Iron and Chromium from 316L Stainless Steel Implants, , Ph.D. Thesis, University of Pennsylvania, Philadelphia; 
Brown, S.A., Farnsworth, L.J., Merritt, K., Crowe, T.D., In vitro and in vivo metal ion release (1988) J. Biomed. Materials Research, 22, pp. 321-338; 
Lucas, L.C., Bearden, L.J., Lemons, J.E., Ultrastructural examination of in vitro and in vivo cells exposed to elements from type 316L stainless steel (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 208-222. , ASTM STP 859 (Fraker, A. C. and Griffin, C. D., Eds.), American Society for Testing and Materials, Philadelphia; 
Merritt, K., Brown, S.A., Biological effects of corrosion products from metals (1985) Corrosion and Degradation of Implant Materials: Second Symposium, pp. 195-207. , ASTM STP859 (Fraker, A. C. and Griffin, C. D., Eds.), American Society for Testing and Materials, Philadelphia; 
Smith, G.K., Black, J., Models for systemic effects of metallic implants (1977) NBS Special Publication 472, pp. 23-28. , Weinstein, A., Eds., U. S. Government Printing Office, Washington D. C; 
Deutman, R., Mulder, T.J., Brian, R., Nater, J.P., Metal sensitivity before and after total hip arthroplasty (1977) J. Bone Jt. Surg., 59 A, pp. 862-865; 
Crosmun, S.T., Mueller, T.R., The determination of chromium (VI) in natural waters by differential pulse polarography (1975) Anal. Chim. Acta, 75, pp. 199-205; 
Golimowski, J., Valenta, P., Nurnberg, H.W., Trace determination of chromium in various water types by adsorption differential pulse polarography (1985) Fresenius Z. Anal. Chem., 322, pp. 315-322; 
Boussemart, M., Van Den Berg, C.M.G., Ghaddaf, M., The determination of the chromium speciation in sea water using catalytic cathodic stripping voltammetry (1992) Anal. Chim. Acta, 262, pp. 103-115; 
Paneli, M., Voulgaropoulos, A.V., Kalcher, K., The catalytic adsorptive stripping voltammetry determination of chromium with TTHA and nitrate (1993) Mikrochim. Acta, 110, pp. 205-215; 
Wang, J., Lu, J.A., Olsen, K., Measurement of ultratrace levels of chromium by adsorptive-catalytic stripping voltammetry in the presence of cupferron (1992) Analyst, 117, pp. 1913-1917; 
Nurnberg, H.W., Studies on toxic trace metals in the environment by advanced polarographic methods (1978) Proc. Analyt. Div. Chem. Soc., 15, pp. 275-283; 
Ottaway, J.M., Fell, G.S., Determination of chromium in biological materials (1986) Pure and Applied Chemistry, 58, pp. 1707-1720; 
Morais, S., Carvalho, G.S., Sousa, J.P., Iron determination in osteoblast-like cell culture medium by adsorptive cathodic stripping voltammetry with a mercury microelectrode (1997) Electroanalysis, 9, pp. 791-795; 
Morais, S., Carvalho, G.S., Sousa, J.P., Determination of nickel in osteoblast-like cell culture medium by adsorptive cathodic stripping voltammetry with a mercury microelectrode (1997) Electroanalysis, 9, pp. 422-426; 
Morais, S., Carvalho, G.S., Sousa, J.P., Potentiometric determination of total and ionised calcium in osteoblast-like cell culture medium (1996) Electroanalysis, 8, pp. 1174-1178; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., In vitro biomineralisation by osteoblast-like cells: Part I- Retardation of tissue mineralisation by metal salts (1998) Biomaterials, 19, pp. 13-21; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., Bruijn, J.D., Van Blitterswijk, C.A., Decreased consumption of Ca and P during in vitro biomineralisation and biologically-induced deposition of Ni and Cr in the presence of stainless steel corrosion products (1998) J. Biomed. Mater. Res., , in press; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., In vitro osteoblastic differentiation of human bone marrow cells in the presence of metal ions (1998) J. Biomed. Mater. Res., , in press; 
Maniatopoulos, C., Sodek, J., Melcher, A.H., Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats (1988) Cell Tissue Res., 254, pp. 317-330; 
Kotz, L., Henze, G., Kaiser, G., Pahlke, S., Veber, M., Tolg, G., Wet mineralisation of organic matrices in glassy carbon vessels in a pressure-bomb system for trace element analysis (1979) Talanta, 26, pp. 681-691; 
Peng, J., Jin, W., Anodic stripping voltammetric determination of trace lead in micro-samples with a mercury-ultramicroelectrode (1992) Anal. Chim. Acta, 264, pp. 213-219; 
Wehmeyer, K.R., Wightman, R.M., Cyclic voltammetry and anodic stripping voltammetry with mercury ultramicroelectrodes (1985) Anal. Chem., 57, pp. 1989-1993; 
Wightman, R.M., Wiptd, O., Voltammetry at ultramicroelectrodes (1989) Electroanalytical Chemistry, 15, pp. 335-336. , Bard, A. J., Ed., Marcel, Dekker, New York; 
Wurfels, M., Jackwerth, E., Stoeppler, M., Residues from biological materials after pressure decomposition with nitric acid Part 3 a (1989) Anal. Chim. Acta, 226, pp. 31-41; 
Morais, S., Sousa, J.P., Fernandes, M.H., Carvalho, G.S., In vitro biomineralisation by osteoblast-like cells: Part II- Characterisation of cellular culture supernatants (1998) Biomaterials, 19, pp. 23-29; 
Wurfels, M., Jackwerth, E., Stoeppler, M., Residues from biological materials after pressure decomposition with nitric acid Part 2 a (1989) Anal. Chim. Acta, 226, pp. 17-30; 
Gilbert, T.R., Clay, A.M., Determination of chromium in sea water by atomic absorption spectrometry (1973) Anal. Chim. Acta, 67, pp. 289-295; 
Ping, L., Matsumoto, K., Fuwa, K., Determination of urinary chromium levels for healthy men and diabetic patients by electric thermal atomic absorption spectrometry (1983) Anal. Chim. Acta, 147, pp. 205-212; 
Torrance, K., Gatford, C., Determination of soluble chromium in simulated PWR coolant by differential-pulse adsorptive stripping voltammetry (1987) Talanta, 34, pp. 939-944; 
Lanza, P., Taddia, M., The differential pulse polarographic determination of chromium in gallium arsenide (1984) Anal. Chim. Acta, 157, pp. 37-44; 
Miller, J.N., Basic statistical methods for analytical chemistry. Part 2: Calibration and regression (1991) Analyst, 116, pp. 3-14; 
Ringbom, A., (1963) Complexation in Analytical Chemistry, pp. 332-333. , Elving, P. J. and Kolthoff, I. M., Eds, Interscience Publishers, New York/London; 
Black, J., Maitin, E.C., Gelman, H., Morris, D.M., Serum concentrations of Cr, Co, and Ni after total hip replacement: A six months study (1983) Biomaterials, 4, pp. 160-164; 
Bartolozzi, A., Black, J., Chromium concentrations in serum, blood clot and urine from patients following total hip arthroplasty (1985) Biomaterials, 6, pp. 2-8; 
Dobbs, H.S., Minski, J., Metal ion release after total hip replacement (1980) Biomaterials, 1, pp. 193-198; 
Katz, S., Salem, H., The toxicology of chromium with respect to its speciation: A review (1993) J. Applied Toxicology, 13, pp. 217-224; 
Bianchi, V., Toso, R.D., Debetto, P., Mechanisms of chromium toxicity in mammalian cell cultures (1980) Toxicology, 17, pp. 219-224; 
Hopkins, L.L., Schwartz, K., Chromium (III) binding to serum proteins, specifically siderophilin (1964) Biochem. Biophys. Acta, 90, pp. 484-491; 
Nurnberg, H.W., The voltammetric approach in trace metal chemistry of natural waters and atmospheric precipitation (1984) Anal. Chim. Acta, 164, pp. 1-21
},
	month = {1998///},
	pages = {101 - 108},
	abstract = {A catalytic cathodic stripping voltammetric procedure for the determination of total chromium in osteoblast-like cell culture medium using a mercury film microelectrode (MFM) was optimised. The method is based on the pre-concentration of the Cr(III)-diethylenetriaminepentaacetic acid (DTPA) complex by adsorption at the potential of -1.00 V (vs. Ag/AgCl) in the presence of 10 x 10},
	keywords = {Acetates, animal cell, Animalia, Animals, article, atomic absorption spectrometry, Bone Marrow Cells, catalysis, Cell culture, Cells, Cultured, Chromium, complex formation, controlled study, Culture Media, culture medium, drug determination, electrochemistry, Electrodes, Hydrogen-Ion Concentration, in vitro study, Kinetics, Mercury, microelectrode, Microelectrodes, Nitrates, nonhuman, osteoblast, Osteoblasts, potentiometry, priority journal, Rabbits, Reproducibility of Results, sensitivity and specificity, Stromal Cells, technique},
	isbn = {0946672X (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-7344255754\&partnerID=40\&md5=16bbb746d7dcdd04b95a01edcc764771},
	author = {Morais, S. and De Carvalho, G.S. and Sousa, J.P.}
}
@article {2590,
	title = {Classification of foetal heart rate sequences based on fractal features},
	journal = {Medical and Biological Engineering and ComputingMed. Biol. Eng. Comput.},
	volume = {36},
	year = {1998},
	note = {Cited By (since 1996): 13Export Date: 21 November 2012Source: ScopusCODEN: MBECDdoi: 10.1007/BF02510743PubMed ID: 9684460Language of Original Document: EnglishCorrespondence Address: Felguiras, C.S.; Instituto de Engenharia Biomedica, Praca Coronel Pacheco 1, 4050 Porto, PortugalReferences: Bernardes, J., (1993) Leitura Automatizada Do Cardiotocograma. Desenvolvimento e Availia{\c c}{\`a}o, , Ph.D. thesis, Faculdade de Medicina da Universidade do Porto, Portugal;Bernardes, J., Moura, C., Marques De S{\'a}, J.P., Pereira Leite, L., Van Geijn, H.P., The Porto system (1994) A Critical Appraisal of Foetal Surveillance, pp. 315-324. , VAN GEIJN H. P. and COPRAY, F. J. A. (Eds.) Elsevier Science, Amsterdam; 
Chaffin, D.G., Goldberg, C.C., Reed, K.L., The dimension of chaos in foetal heart rate (1991) Am. J. Obstet. Gynecol., 165 (5), pp. 1425-1429; 
Dawes, G.S., Visser, G.H.A., Goodman, J.D.S., Redman, C.W.G., Numerical analysis of human foetal heart rate: The quality of ultrasound records (1981) Am. J. Obstet, Gynecol., 141 (1), pp. 43-52; 
Felgueiras, C.S., Marques De S{\'a}, J.P., Bernardes, J., Gama, S., On the reconstruction of foetal heart rate sequences (1996) Actas de Bieng {\textquoteright}96, Sociedade Portuguesa de Engenharia Biom{\'e}dica, Aveiro, Mar{\c c}o, 1996, pp. VII61-VII65; 
Goldberger, A.L., Fractal mechanisms in the electrophysiology of the heart (1992) IEEE Eng. Med. Biol., pp. 47-52. , June; 
Grant, A., Principles for clinical evaluation of methods of perinatal monitoring (1984) J. Perinat. Med., 12, pp. 227-231; 
Grassberger, P., Procaccia, I., Measuring the strangeness of strange attractors (1983) Physica D, 9, pp. 189-208; 
Higushi, T., Approach to an irregular time series on the basis of the fractal theory (1988) Physica D, 31, pp. 277-283; 
Hoyer, D., Liebovitch, L.S., Schmidt, K., Luethke, B., Zwiener, U., Practical problems of determining the dimensions of heart rate data (1997) Med. Biol. Eng. Comput., 35, pp. 27-32; 
Maldelbrot, B.B., Van Ness, J.W., Fractional Brownian motions, fractional noises and applications (1968) SIAM Rev., 10 (4), pp. 422-437; 
Marques De Sa, J.P., Moura, A.C., Bernardes, J., Pereira Leite, L., An{\'a}lise autom{\'a}tica de sinais cardiotocogr{\'a}ficos (1992) Acta M{\'e}dica Portuguesa 11 S{\'e}rie, 5 (1), pp. 28-30; 
Parker, T.S., Chua, L.O., (1989) Practical Numerical Algorithms for Chaotic Systems, , Springer-Verlag; 
Schulman, H., Schulman, S., Lai, P., Schneider, E., Farmakides, G., Dawes, G., Antepartum computerized foetal heart rate, chaos analysis, blood gases and perinatal outcome (1994) J. Maternal-Fetal Invest., 4, pp. 59-63
},
	month = {1998///},
	pages = {197 - 201},
	publisher = {Peter Peregrinus Ltd},
	abstract = {Visual inspection of foetal heart rate (FHR) sequences is an important means of foetal well-being evaluation. The application of fractal features for classifying physiologically relevant FHR sequence patterns is reported. The use of fractal features is motivated by the difficulties exhibited by traditional classification schemes to discriminate some classes of FHR sequence and by the recognition that this type of signal exhibits features on different scales of observation, just as fractal signals do. To characterise the signals by fractal features, two approaches are taken. The first models the FHR sequences as temporal fractals. The second uses techniques from the chaos-theory field and aims to model the attractor based on FHR sequences. The fractal features determined by both approaches are used to design a Bayesian classification scheme. Classification results for three classes are presented; they are quite satisfactory and illustrate the importance of this type of methodology.Visual inspection of foetal heart rate (FHR) sequences is an important means of foetal well-being evaluation. The application of fractal features for classifying physiologically relevant FHR sequence patterns is reported. The use of fractal features is motivated by the difficulties exhibited by traditional classification schemes to discriminate some classes of FHR sequence and by the recognition that this type of signal exhibits features on different scales of observation, just as fractal signals do. To characterise the signals by fractal features, two approaches are taken. The first models the FHR sequences as temporal fractals. The second uses techniques from the chaos-theory field and aims to model the attractor based on FHR sequences. The fractal features determined by both approaches are used to design a Bayesian classification scheme. Classification results for three classes are presented; they are quite satisfactory and illustrate the importance of this type of methodology.
},
	keywords = {article, Bayes Theorem, Cardiology, Chaos techniques, Chaos theory, Classification, Computer aided analysis, Computer simulation, Electrocardiography, Female, Fetal monitoring, fetus, fetus heart rate, FHR classification, FHR sequence, Foetal heart rate sequences, fractal analysis, Fractal features, Fractals, Heart Rate, Fetal, human, Humans, Pattern recognition, Pregnancy, Signal Processing, Computer-Assisted, technique},
	isbn = {01400118 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0032033010\&partnerID=40\&md5=563144fcdb064dd777002155aaa62f73},
	author = {Felgueiras, C.S. and De Marques S{\'a}, J.P. and Bernardes, J. and Gama, S.}
}
@article {2619,
	title = {Characterization of apatite layer formation on P                    2O
                    5-CaO, P
                    2O
                    5-CaO- Na
                    2O, and P
                    2O
                    5-CaO-Na
                    },
	journal = {Journal of Biomedical Materials ResearchJ. BIOMED. MATER. RES.},
	volume = {31},
	year = {1996},
	note = {Cited By (since 1996): 20Export Date: 21 November 2012Source: ScopusCODEN: JBMRBdoi: 10.1002/(SICI)1097-4636(199608)31:4<481::AID-JBM7>3.0.CO;2-HPubMed ID: 8836844Language of Original Document: EnglishCorrespondence Address: Jha, L.J.; IRC in Biomedical Materials, Queen Mary and Westfield College, Mile End Road, London E1 4NS, United KingdomChemicals/CAS: HydroxyapatitesReferences: Hyakuna, K., Yamamuro, T., Kotoura, Y., Oka, M., Kokubo, T., Kushitani, H., Surface reactions of calcium phosphate ceramics to various solutions (1990) J. Biomed. Mater. Res., 24, pp. 471-488;Ebisawa, Y., Kokubo, T., Ohura, K., Yamamuro, T., Bioactivity of CaO{\textperiodcentered}SiO 2-based glasses: In vitro evaluation (1990) J. Mater. Sci.: Mater. Med., 1, pp. 239-244; 
Kokubo, T., Kushitani, H., Ohtsuki, C., Sakka, S., Yamamuro, T., Chemical reaction of bioactive glass and glass ceramics with a simulated body fluid (1992) J. Mater. Sci.: Mater. Med., 3, pp. 79-83; 
Li, P., Yang, Q., Zhang, F., Kokubo, T., The effect of residual glassy phase in a bioactive glass-ceramic on the formation of its surface apatite layer in vitro (1992) J. Mater. Sci.: Mater. Med., 3, pp. 452-456; 
Ohtsuki, C., Aoki, Y., Kokubo, T., Yamamuro, T., Nakamura, T., Characterization of apatite layer formed on bioactive glass-ceramic A-W (1992) Bioceramics, 5, pp. 87-94. , T. Yamamuro, T. Kokubo, and T. Nakamura (eds.), Kokunshi Kankokai, Japan; 
Kokubo, T., Kushitani, H., Ohtsuki, C., Sakka, S., Effects of ions dissolved from bioactive glass-ceramic on surface apatite formation (1993) J. Mater. Sci.: Mater. Med., 4, pp. 1-4; 
Kohri, M., Miki, K., Waite, D., Nakajima, H., Okabe, T., In vitro stability of biphasic calcium phosphate ceramics (1993) Biomaterials, 14, pp. 299-304; 
Andersson, O.H., Kangasniemi, I., Calcium phosphate formation at the surface of bioactive glass in vitro (1991) J. Biomed. Mater. Res., 25, pp. 1019-1030; 
Kokubo, T., Kushitani, H., Sakka, S., Kitsugi, T., Yamamuro, T., Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W (1990) J. Biomed. Mater. Res., 24, pp. 721-734; 
Santos, J.D., Knowles, J.C., Hastings, G.W., Bonfield, W., "Sintered hydroxyapatite compositions and method for the preparation thereof," British Patent No. 9,213,774.4 (1993)Santos, J.D., Reis, R.L., Knowles, J.C., Hastings, G.W., Monteiro, F.J., Microstructural characterisation of glass-reinforced hydroxyapatite composites (1994) Biomaterials, 15, pp. 5-10; 
Santos, J.D., Knowles, J.C., Monteiro, F.J., Hastings, G.W., Development of a glass reinforced hydroxyapatite with enhanced mechanical properties: Physical properties and in vitro studies (1992) Bioceramics, 5, pp. 35-41. , T. Yamamuro, T. Kokubo, and T. Nakamura (eds.), Kokunshi Kankokai, Japan; 
Knowles, J.C., Abrahams, I., Bonfield, W., Effect of reinforcing glass composition on phase transformation and crystallographic parameters in hydroxyapatite (1993) Bioceramics, 6, pp. 191-196. , P. Ducheyne and D. Christiansen (eds.), Butterworth Heinemann, New York; 
Knowles, J.C., Bonfield, W., Development of a glass reinforced hydroxyapatite with enhanced mechanical properties. The effect of glass composition on mechanical properties and its relationship to phase changes (1993) J. Biomed. Mater. Res., 27, pp. 1591-1598; 
Burnie, J., Gilchrist, T., Controlled release glass (C.R.C.) - A new biomaterial (1983) Ceramics in Surgery, pp. 169-176. , P. Vincenzini (ed.), Elsevier, Amsterdam; 
Santos, J.D., Jha, L.J., Monteiro, F.J., Study of surface modifications by x-ray photoelectron spectroscopy on glass reinforced hydroxyapatite composites in a simulated physiological solution (1995) Biomaterials, 16, pp. 521-526; 
Rehman, I., Hench, L.L., Bonfield, W., Comparison of hydroxycarbonate apatite layers on bioactive glasses with human bone (1993) Bioceramics, 6, pp. 123-128. , P. Ducheyne and D. Christiansen (eds.), Butterworth Heinemann, New York; 
Landis, W.J., Martin, J.R., X-ray photoelectron spectroscopy applied to gold-decorated mineral standards of biological interest (1984) J. Vac. Sci. Technol., A2, p. 1108; 
Swift, P., Adventitious carbon - The panacea for energy referencing? (1982) Surface Interface Anal., 4, pp. 47-51; 
Clark, D.T., Thomas, H.R., Applications of ESCA to polymer chemistry. XVII. Systematic investigation of the core levels of single homopolymers (1978) J. Polym. Sci., Polym. Chem. Ed., 16, pp. 791-820; 
Verhoeven, J.A., Van Doveren, H., An XPS investigation of the interface of methane, ethyne, ethene, and ethane with barium surface (1982) Surface Sci., 123, pp. 369-383
},
	month = {1996///},
	pages = {481 - 486},
	publisher = {John Wiley \& Sons Inc},
	abstract = {P                        2O
                        5-based glass hydroxyapatite (HA) composites denoted HA-2 oxide, HA-3 oxide, and HA-4 oxide, were immersed in Hank{\textquoteright}s balanced salt solution for a period of 1, 2, 3, and 4 weeks at 37{\textdegree}C in nonagitated condition for in vitro evaluation. A surface layer was precipitated on the composites that was analyzed using scanning electron microscopy (SEM), X-ray photoelecton spectroscopy, and thin film X-ray diffractometry (TF-XRD). SEM micrographs showed complete coverage of the composite surface by crystallites after immersion of 1 week in the solution, which grew thicker with respect to immersion time. The binding energies measured by XPS indicated apatite formation and the presence of carbonate on the composite surface, showing the newly formed layer was a carbonated apatite. Confirmation of the formed apatite layer was obtained by TF-XRD.
                        2O
                        5-based glass hydroxyapatite (HA) composites denoted HA-2 oxide, HA-3 oxide, and HA-4 oxide, were immersed in Hank{\textquoteright}s balanced salt solution for a period of 1, 2, 3, and 4 weeks at 37{\textdegree}C in nonagitated condition for in vitro evaluation. A surface layer was precipitated on the composites that was analyzed using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy, and thin film X-ray diffractometry (TF-XRD). SEM micrographs showed complete coverage of the composite surface by crystallites after immersion of 1 week in the solution, which grew thicker with respect to immersion time. The binding energies measured by XPS indicated apatite formation and the presence of carbonate on the composite surface, showing the newly formed layer was a carbonated apatite. Confirmation of the formed apatite layer was obtained by TF-XRD.
},
	keywords = {Apatite, article, binding affinity, Biomaterials, bone strength, Calcium Compounds, Carbonates, Ceramic materials, Characterization, chemical composition, Composite materials, Crystallites, electron microscopy, glass, Glass hydroxyapatite composites, Hank balanced salt solution, hydroxyapatite, Hydroxyapatites, immersion, Microscopy, Electron, Scanning, Molecular Structure, Oxides, prosthesis material, Scanning electron microscopy, scanning tunneling microscopy, Spectrum Analysis, Thin film X ray diffractometry, Thin films, X ray diffraction, X ray diffraction analysis, X ray photoelectron spectroscopy, X-Ray Diffraction},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0030222031\&partnerID=40\&md5=1cba84bf7d0e93a4e3a3808849398c78},
	author = {Jha, L.J. and Santos, J.D. and Knowles, J.C.}
}
@article {2621,
	title = {Crystallinity and structural changes in HA plasma-sprayed coatings induced by cyclic loading in physiological media},
	journal = {Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED.},
	volume = {7},
	year = {1996},
	note = {Cited By (since 1996): 13Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1007/BF00122009Language of Original Document: EnglishCorrespondence Address: Reis, R.L.; Dept. of Metallurgical Engineering, FEUP, Univ. of Porto, Rua dos Bragas, 4099 Porto Codex, PortugalReferences: Ji, H., Marquis, P.M., (1993) Biomaterials, 14, p. 64;Pfaff, H.G., Willmann, G., Henne, R., (1993) Proceedings of the 10th European Conference on Biomaterials, p. 110. , Davos, Switzerland, 1993, edited by B. Rahn (European Society for Biomaterials); 
Brown, S.D., (1984) Thin Solid Films, 119, p. 127; 
Geesink, R.G.T., (1988), PhD thesis, Univ. Leiden, The NetherlandsVan Blitterswijk, C.A., Grote, J.J., Kuypers, W., Blok-Van Hoek, C.J.G., Daems, W.Th., (1985) Biomaterials, 6, p. 243; 
Ribeiro, C.C., Barbosa, M.A., (1991) Bioceramics 4, p. 145. , edited by W. Bonfield, G. W. Hastings and K. Tanner (Butterworth-Heinemann London); 
Ducheyne, P., Healy, K.E., (1988) J. Biomed. Mater. Res., 22, p. 1137; 
Whitehead, R.Y., Lucas, L.C., Lacefield, W.R., (1993) Clin. Mater., 12, p. 31; 
De Bruijn, J.D., Davies, J.E., Flach, J.S., De Groot, K., Van Blitterswijk, C.A., (1992) Proceedings of the 4th World Congress on Biomaterials - Workshop on Biomaterials and Tissue Interactions, p. 23. , edited by U. Gross (Berlin); 
Morita, M., Sasada, T., Hayashi, H., Tsukamoto, Y., (1988) J. Biomed. Mater. Res., 22, p. 529; 
Brown, S.A., Farsnworth, L.J., Merritt, K., Crowe, T.D., (1988) J. Biomed. Mater. Res., 22, p. 321; 
Kummer, F., Jaffe, W., (1992) J. Appl. Biomater., 3, p. 211; 
Reis, R.L., Monteiro, F.J., Garrido, A., (1992) Bioceramics 5, p. 25. , Kyoto, November, 1992, edited by T. Yamamura, T. Kokubo and T. Nakamura (Kobunshi Kankokai, Tokyo); 
Reis, R.L., Monteiro, F.J., (1993) Bioceramics 6, p. 413. , Philadelphia, USA, 1993, edited by P. Ducheyne and D. Christiansen (Butterworth-Heinemann. New York); 
Reis, R.L., Monteiro, F.J., Hastings, G.W., (1994) J. Mater. Sci. Mater. Med., 5, p. 457; 
Reis, R.L., Monteiro, F.J., (1995) Proceedings of the NATO/ASI on Materials Science and Implant Orthopaedic Surgery II, p. 163. , Chania, Greesce, June 1994. edited by R. Kossowsky and N. Kossowsky (May, NATO/ASI series E: Applied Sciences, Kluwer Press, Amsterdam); 
Ji, H., Ponton, C.B., Marquis, P.M., (1992) J. Mater. Sci. Mater. Med., 3, p. 283; 
Weng, J., Liu, X., Zhang, X., Ma, Z., Ji, X., Zyman, Z., (1993) Biomaterials, 14, p. 578; 
De Groot, K., Klein, C.A.P.T., Wolke, J.G.C., Blieck-Hogervorst, J.M.A., (1990) Handbook of Bio-active Ceramics, 2, p. 133. , edited by T. Yamamuro, L. L. Hench and J. Wilson (CRC Press, Boca Raton, FL); 
Del{\'e}crin, J., Szumuckler-Moncler, S., Daculsi, G., Rieu, J., Duquet, B., (1991) Bioceramics 4, p. 311. , London, September, 1991, edited by W. Bonfield, G. W. Hastings and K. E. Tanner (Butterworth-Heinemann, London); 
De Bruijn, J.D., Flach, J.S., Leenders, H., Van der Brink, J., Van Blitterswijk, C., (1992) Bioceramics 5, p. 259. , Kyoto, November, 1992, edited by T. Yamamura, T. Kokubo and T. Nakamura (Kobunshi Kankokai, Tokyo)
},
	month = {1996///},
	pages = {407 - 411},
	publisher = {Chapman \& Hall Ltd},
	abstract = {In this study hydroxyapatite plasma-sprayed Ti-6A1-4V was characterized by X-ray diffraction (XRD) after being cyclically loaded, while immersed in two different testing environments: Hank{\textquoteright}s balanced salt solution (HBSS) and an isotonic saline solution (0.15m NaCl). An original method was developed to test these systems under cyclic bending, while immersed in the testing solution. The crystallinity of the coating was calculated, before and after testing. Crystallinity changes were related to coating dissolution rates, measured by atomic absorption spectroscopy (AAS) and by a photocolourimetric method using ascorbic acid. Structural changes induced either by the plasma-spraying procedure or due to corrosion-fatigue tests, namely a preferential dissolution of the coating{\textquoteright}s amorphous phase, were also found in X-ray diffraction patterns. Long-term immersion tests, without any applied load, were carried out for assessing cyclic loading effects. An immersion for 2 years in static conditions was found to be equivalent to a cyclic test of 27.8 h in the same solution, as measured by the changes on the coating structure and by the amount of coating material lost to the solution.In this study hydroxyapatite plasma-sprayed Ti-6Al-4V was characterized by X-ray diffraction (XRD) after being cyclically loaded, while immersed in two different testing environments: Hank{\textquoteright}s balanced salt solution (HBSS) and an isotonic saline solution (0.15M NaCl). An original method was developed to test these systems under cyclic bending, while immersed in the testing solution. The crystallinity of the coating was calculated, before and after testing. Crystallinity changes were related to coating dissolution rates, measured by atomic absorption spectroscopy (AAS) and by a photocolourimetric method using ascorbic acid. Structural changes induced either by the plasma-spraying procedure or due to corrosion-fatigue tests, namely a preferential dissolution of the coating{\textquoteright}s amorphous phase, were also found in X-ray diffraction patterns. Long-term immersion tests, without any applied load, were carried out for assessing cyclic loading effects. An immersion for 2 years in static conditions was found to be equivalent to a cyclic test of 27.8 h in the same solution, as measured by the changes on the coating structure and by the amount of coating material lost to the solution.
},
	keywords = {Absorption spectroscopy, article, ascorbic acid, atomic absorption spectrometry, Atomic absorption spectroscopy, balanced salt solution, Bending (deformation), Calculations, Characterization, Corrosion fatigue tests, crystal structure, Crystallinity, Cyclic bending, Dissolution, Fatigue testing, Hanks balanced salt solution, hydroxyapatite, Hydroxyapatite plasma sprayed coatings, immersion, implant, Isotonic saline solution, loading test, Long term immersion tests, materials testing, Modification, Photocolourimetric method, photometry, Plasma spraying, priority journal, sodium chloride, Sprayed coatings, Titanium alloys, Titanium aluminum vanadium alloys, X ray crystallography, X ray diffraction},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0030199724\&partnerID=40\&md5=b47480a26cc5b628bc98bc98034c6c98},
	author = {Reis, R.L. and Monteiro, F.J.}
}
@article {2637,
	title = {Computerized fetal heart rate analysis in labour based on 2-s sampling. Can it proceed with confidence? [1]},
	journal = {European Journal of Obstetrics Gynecology and Reproductive BiologyEUR. J. OBSTET. GYNECOL. REPROD. BIOL.},
	volume = {63},
	year = {1995},
	note = {Cited By (since 1996): 2Export Date: 21 November 2012Source: ScopusCODEN: EOGRAdoi: 10.1016/0301-2115(95)02215-SPubMed ID: 8674558Language of Original Document: EnglishCorrespondence Address: Bernardes, J.; Instituto Engenharia Biomedica, Praca Coronel Pacheco 1, 4000 Porto, Portugal},
	month = {1995///},
	pages = {105 - 107},
	keywords = {computer analysis, Computers, Female, Fetal monitoring, fetus, fetus heart rate, Heart Rate, Fetal, human, labor, Labor, Obstetric, letter, measurement, Pregnancy, priority journal, sampling, Time Factors},
	isbn = {03012115 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0028856781\&partnerID=40\&md5=d0c302fff078192103c46bde7d37746f},
	author = {Bernardes, J. and Garrido, A. and Chang, A. and Sahota, D.S. and Reed, N.N. and James, D.K. and Mohajer, M.P.}
}
