| Title | Flow cytometry analysis of effects of glass on response of osteosarcoma cells to plasma-sprayed hydroxyapatite/CaO-P 2O
5 coatings
|
| Publication Type | Journal Article |
| 1999 |
| Authors | Ferraz, MP, Knowles, JC, Olsen, I, Monteiro, FJ, Santos, JD |
| Journal | Journal of Biomedical Materials ResearchJ. Biomed. Mater. Res. |
| Volume | 47 |
| Issue | 4New York, NY, United States |
| Pagination | 603 - 611 |
| Date Published | 1999/// |
| 00219304 (ISSN) |
| Animals, Antibodies, antigen expression, article, Bioassay, Biomaterials, Bone cells, Bone sialoprotein, Bone Substitutes, calcium phosphate, cancer cell culture, Cell Division, cell function, cell granule, cell growth, cell proliferation, Cell size, Cells, Coated Materials, Biocompatible, Collagen, collagen type 1, connective tissue, controlled study, dentistry, enzyme activity, Enzymes, Fibronectin, flow cytometry, Flow cytometry analysis, glass, Growth kinetics, human, human cell, Humans, hydroxyapatite, Hydroxyapatite-glass, Hydroxyapatites, Incubation, Mitochondrial enzyme, orthopedics, Osteoblasts, osteonectin, Osteopontin, Osteosarcoma, osteosarcoma cell, Osteosarcoma cells, Phosphates, Proliferation, Proteins, Rabbits, sialoprotein, Spectrophometric assay, Tissue culture, Tumor Cells, Cultured |
| Multilayered coatings composed of mixtures of hydroxyapatite (HA) and P 2O
5-based bioactive glasses offer potential clinical benefits in orthopedic and dental surgery. In this study double-layer plasma-sprayed coatings were prepared and the biological response evaluated in tissue culture using two human osteosarcoma cell lines, MG63 and HOS TE85 (HOS). The cells were cultured on the materials and the effects on cell growth were determined using a spectrophometric assay of a mitochondrial enzyme that is active in viable cells. While none of the materials influenced the growth of the MG63 cells, the HOS cells appeared to undergo less proliferation on all the HA materials. Flow cytometry analysis was carried out using rabbit antibodies against osteonectin, osteopontin, bone sialoprotein, fibronectin, and collagen type I to measure the effects of the materials on key cellular functions. The results showed that the materials downregulated the expression of these extracellular matrix antigens by MG63 cells whereas they had less effect on the HOS cells compared to the same cells incubated on a plastic surface. Notably, with both cell lines the composite with the higher percentage of glass restored the production of connective tissue proteins to levels that were more similar to those present in the control cells.
In this study, double-layer plasma-sprayed coatings were prepared and the biological response evaluated in tissue culture using two human osteosarcoma cell lines, MG63 and HOS TE85 (HOS). The materials downregulated the expression of the extracellular matrix antigens by MG63 cells whereas they had less effect on the HOS cells compared to the same cells incubated on a plastic surface. Notably, with both cell lines the composite with the higher percentage of glass restored the production of connective tissue proteins to levels that were more similar to those present in the control cells.
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