| Title | Improving chitosan-mediated gene transfer by the introduction of intracellular buffering moieties into the chitosan backbone |
| Publication Type | Journal Article |
| 2009 |
| Authors | Moreira, C, Oliveira, H, Pires, LR, Simões, S, Barbosa, MA, Pêgo, AP |
| Journal | Acta BiomaterialiaActa Biomater. |
| Volume | 5 |
| Issue | 8 |
| Pagination | 2995 - 3006 |
| Date Published | 2009/// |
| 17427061 (ISSN) |
| article, beta galactosidase, Biocompatible Materials, Buffers, Cell Line, cell transport, Chemical structure, chitosan, coacervation, complex formation, controlled study, Cytotoxicity, Diffusion, DNA, Drug Carriers, Drug Compounding, endosome, Gene delivery, Gene transfer, genetic transfection, human, human cell, Humans, imidazole, Kidney, materials testing, nanomaterial, nanoparticle, priority journal, protein expression, proton transport, Tissue engineering, Transfection |
| Chitosan was functionalized with imidazole moieties (CHimi) with the aim of improving its buffering capacity and promoting the endosomal escape ability of chitosan-DNA complexes, ultimately increasing their transfection efficiency. 5.6%, 12.9% and 22.1% of the glucosamine residues of chitosan were substituted. Complexes with different molar ratios of primary amines to DNA phosphate anion (N/P) were prepared by a coacervation method. For an N/P > 3, CHimi polymers are able to complex electrostatically with DNA and condense it into positively charged nanostructures (average size 260 nm and zeta potential +16 mV at pH 5.5). In the concentration range 2.5-100 μg ml |
| http://www.scopus.com/inward/record.url?eid=2-s2.0-70349162856&partnerID=40&md5=fb6672dd0cf06d6ced21eec115b597d2 |
