| Title | Inflammatory cell recruitment and adhesion to methyl-terminated self-assembled monolayers: Effect of implantation time |
| Publication Type | Journal Article |
| 2005 |
| Authors | Barbosa, JN, Barbosa, MA, Águas, AP |
| Journal | Microscopy Research and TechniqueMicrosc. Res. Tech. |
| Volume | 66 |
| Issue | 1 |
| Pagination | 37 - 42 |
| Date Published | 2005/// |
| 1059910X (ISSN) |
| Air-pouch model, animal experiment, animal model, Animalia, Animals, article, biomaterial, cell adhesion, Cell Count, cell density, cell surface, Cell-surface interactions, Coated Materials, Biocompatible, Contact angle, controlled study, gold, implantation, Implants, Experimental, Inflammation, inflammatory cell, Inflammatory responses, leukocyte adherence, Leukocytes, male, materials testing, methyl group, Mice, Microscopy, Electron, Scanning, mononuclear cell, mouse, neutrophil, nonhuman, priority journal, Scanning electron microscopy, Self-assembled monolayers, Surface Properties, thiol derivative, Time Factors |
| The contribution of methyl groups in implant-triggered inflammation was investigated in vivo using self-assembled monolayers (SAMs) of alkanethiols on gold. The CH 3-coated implants were inserted in an air-pouch cavity induced in BALB/c mice. The in situ inflammatory response was monitored 24, 48, and 72 hours later. Inflammatory cells recovered from the air pouches were counted and observed by light microscopy. The cellularity of the implant surfaces was defined by scanning electron microscopy (SEM). In comparison with gold implants, the CH
3-coated SAMs recruited a significantly higher number of inflammatory cells. Polymorphonuclear leukocytes (PMN) were more numerous than mononuclear cells (Mo) in the exudates recovered from the air pouches with CH
3-coated SAMs. The opposite PMN/Mo proportion was observed in air pouches of the two control groups (mice receiving gold implants or sham-operated animals). A low density of adherent cells was seen on CH
3-coated implants, with no significant quantitative differences during the time course of the study. In contrast, the gold-coated surfaces were covered with numerous cells during all of the 3 days of the inflammation. In conclusion, implants with CH
3 surfaces are likely to induce PMN-dominated local acute inflammation but these surfaces are not associated with a significant adherence of leukocytes to the implant. © 2005 Wiley-Liss, Inc.
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| http://www.scopus.com/inward/record.url?eid=2-s2.0-17144372986&partnerID=40&md5=1d2d00c4efe22d960872f5e785c7d4c7 |
