| Title | Inhibition and stimulation of enzymatic activities of human fibroblasts by corrosion products and metal salts |
| Publication Type | Journal Article |
| 1996 |
| Authors | Carvalho, GS, Castanheira, M, Diogo, I, Abreu, AM, Sousa, JP, Loon, JA, Van Blitterswijk, CA |
| Journal | Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED. |
| Volume | 7 |
| Issue | 2London, United Kingdom |
| Pagination | 77 - 83 |
| Date Published | 1996/// |
| 09574530 (ISSN) |
| 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, Absorption spectroscopy, Acid phosphatase, article, atomic absorption spectrometry, Atomic spectroscopy, Bioassay, Cell culture, Cell morphology, cell proliferation, cell structure, cell viability, Chromium, controlled study, Corrosion, Corrosion products, cytology, DNA, Enzymatic assays, Enzyme Activation, enzyme activity, Enzyme inhibition, fibroblast, human, human cell, Ions, Iron, metal, Metal ion accumulation, Metal salts, Metals, neutral red, Nickel, priority journal, Salts, Skin, skin fibroblast, Stainless steel, Steel corrosion |
| Fibroblasts from normal human skin were cultured for a period of 21 days in the absence or in the presence of metal ions. The effects of stainless steel (SS) corrosion products were compared to the effects of iron, chromium and nickel ions used either separately (Fe, Cr, or Ni solutions) or combined (Fe+Cr+Ni solution). At several periods of time (4, 7, 14 and 21 days) the cell cultures were analysed for the following parameters: (a) metal ion accumulation by atomic absorption spectrometry; (b) cell morphology and viability by the neutral red assay; (c) cell proliferation by DNA assessment, and enzyme activity by both (d) MTT reduction and (e) acid phosphatase activity. Results showed that SS-corrosion products and the corresponding metal ions combined at the same concentrations, Fe+Cr+Ni solution, had opposite effects on fibroblast cultures. In fact, SS-corrosion products caused no apparent effects on cell morphology nor on cell proliferation whereas Fe+Cr+Ni solution stimulated both neutral red uptake and cell proliferation. The enzymatic assays showed that SS-corrosion products caused inhibition of both MTT reduction and acid phosphatase activity in contrast to Fe+Cr+Ni solution which stimulated their activity. Furthermore, in all biological parameters studied, a strong association was observed between the effects of Fe+Cr+Ni solution and Cr alone, suggesting that Cr was the metal ion mostly involved in the stimulatory effects of the combined solution.Fibroblasts from normal human skin were cultured for 21 days in the absence, or presence, of metal ions. The effects of stainless steel (SS) corrosion products were compared to the effects of iron, chromium, and nickel ions used either separately or combined. At different periods of time, the cell cultures were analyzed for the following parameters: metal ion accumulation by atomic absorption spectrometry; cell morphology and viability by the neutral red assay; cell proliferation by DNA assessment; and enzyme activity by both MTT reduction and acid phosphatase activity. Results show that SS-corrosion products and the corresponding metal ions combined at the same concentrations, Fe+Cr+Ni solution, have opposite effects on fibroblast cultures.
|
| http://www.scopus.com/inward/record.url?eid=2-s2.0-0030087165&partnerID=40&md5=a26cd67a43c1fef54a5df943014fd7c6 |
