| Title | Micropatterned silica thin films with nanohydroxyapatite micro-aggregates for guided tissue regeneration |
| Publication Type | Journal Article |
| 2012 |
| Authors | Carvalho, A, Pelaez-Vargas, A, Gallego-Perez, D, Grenho, L, Fernandes, MH, De Aza, AH, Ferraz, MP, Hansford, DJ, Monteiro, FJ |
| Journal | Dental MaterialsDent. Mater. |
| Volume | 28 |
| Issue | 12 |
| Pagination | 1250 - 1260 |
| Date Published | 2012/// |
| 01095641 (ISSN) |
| Adhesion, AFM, Aggregates, Bacteria, Bacterial adhesion, Bioactive nanoparticles, Biological characterization, Biological materials, Biological response, Biomaterials, Cell culture, Contact angle, Dental prostheses, Flowcharting, FTIR/ATR, Guided tissue regeneration, Lithography, Mesenchymal stem cell, Micro patterning, Micro-texture, Microaggregates, Micropatterned, Micropatterned surface, Micropatterning, Nano-HA, Nano-hydroxyapatite, S. mutans, SEM/EDS, Silica, Silica thin films, Soft-lithography, Sol-gel, Sol-gel process, Sol-gels, Streptococcus mutans, Surface modification techniques, Surfaces, Thin films |
| Surface modification of biomaterials has been shown to improve the biological response to dental implants. The ability to create a controlled micro-texture on the implant via additive surface modification techniques with bioactive nanohydroxyapatite (nanoHA) may positively influence guided tissue regeneration. Objective: The main goal of this study was to produce micro-fabricated SiO 2 surfaces modified with nanohydroxyapatite particles and to characterize their influence on the biological response of Human Dental-Pulp Mesenchymal Stem Cells (hDP-MSCs) and Streptococcus mutans. Materials and methods: A combined methodology of sol-gel and soft-lithography was used to produce micropatterned SiO
2 thin films with different percentages of nanoHA micro-aggregates. The surfaces were characterized by SEM/EDS, FT-IR/ATR, AFM, XPS quantitative elemental percentage and contact angle measurements. Biological characterization was performed using hDP-MSCs cultures, while Streptococcus mutans was the selected microorganism to evaluate the bacterial adhesion on the thin films. Results: Micropatterned SiO
2 surfaces with 0%, 1% and 5% of nanoHA micro-aggregates were successfully produced using a combination of sol-gel and soft-lithography. These surfaces controlled the biological response, triggering alignment and oriented proliferation of hDP-MSCs and significant differences in the adhesion of S. mutans to the different surfaces. Significance: The micropatterned surfaces exhibited biocompatible behavior that induced an oriented adhesion and proliferation of hDP-MSCs while SiO
2 presented low bacterial adhesion. These results show that the combination of sol-gel with soft-lithography is a good approach to create micropatterned surfaces with bioactive nanoparticles for guided tissue regeneration. © 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
|
| http://www.scopus.com/inward/record.url?eid=2-s2.0-84867841771&partnerID=40&md5=f33477063f16e99361687470c94d7289 |
