| Title | Mineralization in serially passaged human alveolar bone cells |
| Publication Type | Journal Article |
| 1997 |
| Authors | Fernandes, MH, Costa, MA, Carvalho, GS |
| Journal | Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED. |
| Volume | 8 |
| Issue | 2London, United Kingdom |
| Pagination | 61 - 65 |
| Date Published | 1997/// |
| 09574530 (ISSN) |
| Acid phosphatase, Acid resistance, alkaline phosphatase, alveolar bone, article, ascorbic acid, Bioassay, Biocompatibility, Biomaterials, Bone, bone cell, Bovinae, Cell culture, cell proliferation, Characterization, Chemical reactions, controlled study, culture medium, dexamethasone, Foetal bovine serum, glycerophosphate, Histochemical assays, histochemistry, human, Human alveolar bone cells, human cell, mineralization, Minimal essential medium, Phosphates, priority journal, Proteins, tartaric acid, Tartraric acid resistant phosphatase, Tissue culture |
| Well-characterized human bone cell cultures have been regarded as a useful tool to study bone control mechanisms and also to analyse bone/biomaterials interactions. In the present study, human alveolar bone cells were cultured in a-minimal essential medium (α-MEM) containing 10% foetal bovine serum (FBS), 50 μg/ml ascorbic acid, 10 mM sodium β-glycerophosphate and either in the presence or in the absence of 10 nM dexamethasone (Dexa). Cultures were characterized concerning cell viability/proliferation, alkaline phosphatase (ALP), acid phosphatase (ACP) and tartraric acid resistant phosphatase (TRAP) activities, and formation of mineralized areas. Cell proliferation increased gradually for approximately 20 days. In the presence of Dexa, cells formed isolated or interconnected multilayered clusters that increased with culture time. Histochemical assays revealed strong positive reactions for ALP and calcium and phosphates deposits, mainly in relation to cells associated with the clusters. High levels of ALP activity (biochemical determination) were observed. Cells cultured in the absence of Dexa showed significantly lower ALP activity and no calcium and phosphates deposits were present. Serially passaged cells kept the proliferation rate constant but a decrease in ALP activity was observed either in the presence or in the absence of Dexa. The ability to form mineralized areas (cultures fed with Dexa) also decreased on serial subculture.Well-characterized human bone cell cultures have been regarded as a useful tool to study bone control mechanisms and also to analyse bone/biomaterials interactions. In the present study, human alveolar bone cells were cultured in α-minimal essential medium (α-MEM) containing 10% foetal bovine serum (FBS), 50 μg/ml ascorbic acid, 10 mM sodium β-glycerophosphate and either in the presence or in the absence of 10 nM dexamethasone (Dexa). Cultures were characterized concerning cell viability/proliferation, alkaline phosphatase (ALP), acid phosphatase (ACP) and tartaric acid resistant phosphatase (TRAP) activities, and formation of mineralized areas. Cell proliferation increased gradually for approximately 20 days. In the presence of Dexa, cells formed isolated or interconnected multilayered clusters that increased with culture time. Histochemical assays revealed strong positive reactions for ALP and calcium and phosphates deposits, mainly in relation to cells associated with the clusters. High levels of ALP activity (biochemical determination) were observed. Cells cultured in the absence of Dexa showed significantly lower ALP activity and no calcium and phosphates deposits were present. Serially passaged cells kept the proliferation rate constant but a decrease in ALP activity was observed either in the presence or in the absence of Dexa. The ability to form mineralized areas (cultures fed with Dexa) also decreased on serial subculture.
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