| Title | Molecular epidemiology of pseudomonas aeruginosa clinical isolates from Portuguese Central Hospital |
| Publication Type | Journal Article |
| 2008 |
| Authors | Fonseca, AP, Correia, P, Extremina, CI, Sousa, JC, Tenreiro, R, Barros, H |
| Journal | Folia MicrobiologicaFolia Microbiol. |
| Volume | 53 |
| Issue | 6 |
| Pagination | 540 - 546 |
| Date Published | 2008/// |
| 00155632 (ISSN) |
| antibiotic resistance, article, bacterial DNA, cross infection, disease transmission, DNA, Bacterial, Drug Resistance, Microbial, Electrophoresis, Gel, Pulsed-Field, epidemiology, genetics, genotype, Hospitals, Teaching, human, Humans, isolation and purification, microbiology, polymerase chain reaction, Portugal, Pseudomonas aeruginosa, Pseudomonas infection, Pseudomonas Infections, pulsed field gel electrophoresis, Sampling Studies, statistics, teaching hospital |
| The relatedness between clinical isolates of P. aeruginosa obtained from patients during their stay in a Portuguese Central Hospital was evaluated. Genotypic fingerprinting (M13-PCR), phenotypic methods (biotyping and antibiotyping) and epidemiological information (spatial and temporal links) were used to evaluate the relatedness between 88 clinical isolates (68 patients), selected randomly out of 189. Sixty-two M13 types were found, 12 of them containing isolates from more than one patient. Thirty-four antibiotypes were found, as well as a significant association (p < 0.05) between epidemic isolates and multiresistance patterns. The nosocomial transmission of P. aeruginosa strains may be limited since M13 typing demonstrated a high degree of diversity among all the isolates, suggesting the occurrence of mainly independent infectious episodes. The results show the possible occurrence of cross-acquisition, cross-colonization and cross-infection and suggest an epidemic population structure for P. aeruginosa in this hospital. |
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