| Title | Mouse inflammatory response to stainless steel corrosion products |
| Publication Type | Journal Article |
| 1994 |
| Authors | Tracana, RB, Sousa, JP, Carvalho, GS |
| Journal | Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED. |
| Volume | 5 |
| Issue | 9-10London, United Kingdom |
| Pagination | 596 - 600 |
| Date Published | 1994/// |
| 09574530 (ISSN) |
| animal cell, animal experiment, Animalia, article, Assays, Cell Count, Cells, Chromium, Chronoamperometry, controlled study, Corrosion, Dissolution, follow up, immune response, immunology, Inflammation, Inflammatory response, Iron, Lymphocyte, Lymphocytes, macrophage, Macrophages, male, metal implantation, Mice (animal), molybdenum, mouse, Nickel, nonhuman, Peritoneal cavity, Polymorphonuclear, polymorphonuclear cell, priority journal, Stainless steel, time, Tissue, Wright stained cytocentrifuge |
| Corrosion occurs regularly following long-term implantation of stainless steel. Little is known about the inflammatory and immunological potential of stainless steel corrosion products. AISI 316L stainless steel was anodically dissolved in a physiologically solution, HBSS, through a chronoamperometric process by imposing an external constant current of 0.5 mA. The solution, containing 245 μg of Fe, 112 μg of Cr, 75 μg of Ni and 13 μg of Mo, was injected in the peritoneal cavity of male C57BL/6 mice. Five animals were used per survival period of time: 4, 16, 24, and 48 h, 1, 2, 4, 8 and 16 weeks. Three control mice per survival period of time were injected with HBSS. For each assay, peritoneal samples were analysed not only for the total number of cells but also for the percentages of macrophages, lymphocytes and polymorphonuclear (PMN) cells, which were estimated by differential counting on Wright-stained cytocentrifuge preparations. Our follow-up study showed that stainless steel corrosion products induced an acute inflammatory response for a period of one week as demonstrated by the influx of PMN cells and macrophages. In contrast neither a chronic inflammation nor an immune response was observed indicating that the stainless steel solution caused a minor tissue response.A follow-up study of the changes to mouse peritoneal cell populations, succeeding intraperitoneal injection of stainless steel corrosion products, in order to assess the inflammatory response to such metals was presented. AISI stainless steel was anodically dissolved in a physiologically solution, Hank's balanced salt solution (HBSS), which was injected in the peritoneal cavity of male mice. Five animals were designated per survival time interval of 4, 16, 24, and 48 h, 1, 2, 4, 8 and 16 weeks. Three control mice per survival period of time were treated with HBSS. For each assay, peritoneal samples were tested for total number of cells and percentages of macrophages, lymphocytes and polymorphonuclear (PMN) cells. The follow-up study revealed that stainless steel corrosion products induced an acute inflammatory response for a duration of one week as shown by the influx of PMN cells and macrophages. In contrast, neither a chronic inflammation nor an immune response was noted suggesting that stainless steel solution caused a minor tissue response.
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