| Title | Neural cell growth on TiO 2 anatase nanostructured surfaces
|
| Publication Type | Journal Article |
| 2009 |
| Authors | Collazos-Castro, JE, Cruz, AM, Carballo-Vila, M, Lira-Cantú, M, Abad, L, del Pino, ÁP, Fraxedas, J, San Juan, A, Fonseca, C, Pêgo, AP, Casañ-Pastor, N |
| Journal | Thin Solid FilmsThin Solid Films |
| Volume | 518 |
| Issue | 1 |
| Pagination | 160 - 170 |
| Date Published | 2009/// |
| 00406090 (ISSN) |
| Amorphous phasis, Anatase, Anti-inflammatory activity, Biomedical applications, Borosilicate glass, Cell culture, cell density, Cell membranes, cell survival, Chemical compositions, Coatings, Conducting substrates, Conductive films, Electrochemical properties, Gelation, Growth kinetics, Heat treatment, Iso-propoxide, Low temperature phasis, Nano-structured, Nanometrics, Nanostructured surface, Nanostructures, Neural cell, Neural cells, Neurite extension, Neurite growth, Neurites, Neurons, Oxide layer, polylysine, Surface topography, Thermal treatment, TiO, Titanium, Titanium dioxide, Titanium oxide, Titanium oxides |
| Titanium oxides have anti-inflammatory activity and tunable electrochemical properties that make them attractive materials for biomedical applications. This work investigated the compatibility of nanometric coatings of low-temperature phases of TiO 2 with neurons in 4-day and 10-day cultures, using different cell densities to quantify cell survival and neurite extension. TiO
2 films were prepared by sol-gel and thermal treatment (250-450 °C) of hydrolyzed titanium tetra-isopropoxide on electrically conducting or insulating slides. The conducting substrates were not passivated by the nanometric oxide layer and could be used as electrodes. Characterization of the films showed nano-structured TiO
2 containing exclusively Ti
+4 valence in anatase and amorphous phases. When coated with polylysine, all films permitted good neuron attachment and survival for at least ten days in culture. However, they generally reduced neurite growth compared to cell culture borosilicate glass, with dendrites more affected than axons. The analyses of surface topography, hydrophilicity, charge and chemical composition revealed that TiO
2 chemistry was the main factor responsible for neurite inhibition. © 2009 Elsevier B.V. All rights reserved.
|
| http://www.scopus.com/inward/record.url?eid=2-s2.0-69649101935&partnerID=40&md5=08dd6874d6b2e9bbe7310add08057bc7 |
