INEB
INEB
TitleNickel Determination in Osteoblast-Like Cell Culture Medium by Adsorptive Cathodic Stripping Voltammetry with a Mercury Microelectrode
Publication TypeJournal Article
1997
AuthorsMorais, S, Carvalho, GS, Sousa, JP
JournalElectroanalysisElectroanalysis
Volume9
Issue5
Pagination422 - 426
Date Published1997///
10400397 (ISSN)
Cell culture medium, Mercury film microelectrode, Nickel
The purpose of this study is to investigate the influence of nickel, which is an alloying element in commonly used metallic biomaterials, on the biomaterials mineralization process. An electrochemical method was developed to quantify this metal ion in osteoblast-like cell culture medium (OST) by performing adsorptive cathodic stripping voltammetry (CSV) with dimethylglyoxime (DMG) at a mercury film microelectrode (MFM). The optimized analytical conditions and the square-wave CSV parameters for the analysis are: DMG concentration: 5.00 × 10
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