| Title | TiO 2 type influences fibronectin adsorption
|
| Publication Type | Journal Article |
| 2005 |
| Authors | Sousa, SR, Moradas-Ferreira, P, Barbosa, MA |
| Journal | Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med. |
| Volume | 16 |
| Issue | 12 |
| Pagination | 1173 - 1178 |
| Date Published | 2005/// |
| 09574530 (ISSN) |
| Adsorption, adsorption kinetics, article, binding affinity, Biointegration, Biomaterials, Cells, chemistry, conference paper, Contact angles, Fibronectin, Fibronectins, human, Human fibronectin (FN), Humans, hydrogen peroxide, hydrophilicity, hydrophobicity, Implants (surgical), iodine 125, isotherm, Isotherms, isotope labeling, physical chemistry, priority journal, protein binding, Proteins, Radiolabelling, solution and solubility, Solutions, surface property, Titanium, Titanium dioxide, wettability, X ray photoelectron spectroscopy |
| Human fibronectin (FN) plays a key role in the biointegration of implants as the success depends on adsorption of proteins like FN [1]. Indeed FN can be an intermediary between the biomaterial surface and cells. The adsorption of human fibronectin (FN) on commercially pure titanium with a titanium oxide layer formed in a H 2O
2 solution (TiO
2 cp) and TiO
2 sputtered on Si (TiO
2 sp) was studied. Adsorption isotherms and the work of adhesion were assessed by wettability studies, X-ray photoelectron spectroscopy (XPS), and by radiolabelling of FN with
125I,
125I-FN. Exchangeability of bound FN by free FN, was also evaluated by the radiolabelling technique. Contact angle determinations have shown that FN displays higher affinity for the TiO
2 cp surface than for the TiO
2 sp. As expected from the surface free energy values, the work of adhesion of FN is higher for the TiO
2 cp substrate, the more hydrophilic one, and lower for the TiO
2 sp substrate, the more hydrophobic one. The adsorption isotherms were evaluated by two different techniques: radiolabelling of FN (
125I-FN) and XPS. TiO
2 cp adsorbs more FN than the TiO
2 sp surfaces as shown by the radiolabelling data. FN molecules are also more strongly attached to the former surface as indicated by the work of adhesion and by the exchangeability studies. Results using
125I-FN also suggests that FN adsorbs as a multilayer for FN concentrations in solution higher than 100 μg/mL. © 2005 Springer Science + Business Media, Inc.
|
| http://www.scopus.com/inward/record.url?eid=2-s2.0-29144439699&partnerID=40&md5=f98f8448d034d8f8627d752516e3afbd |
