| Title | In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes |
| Publication Type | Journal Article |
| 2005 |
| Authors | Shirosaki, Y, Tsuru, K, Hayakawa, S, Osaka, A, Lopes, MA, Santos, JD, Fernandes, MH |
| Journal | BiomaterialsBiomaterials |
| Volume | 26 |
| Issue | 5 |
| Pagination | 485 - 493 |
| Date Published | 2005/// |
| 01429612 (ISSN) |
| alkaline phosphatase, article, artificial membrane, Biocompatibility, Biocompatible Materials, Bone Neoplasms, Calcium, calcium chloride, cell adhesion, Cell attachments, Cell culture, cell differentiation, Cell Division, Cell Line, Tumor, chitosan, chitosan derivative, controlled study, cross linking, Cross-Linking Reagents, Crosslinking, Cytocompatibility, Fourier transform infrared spectroscopy, human, human cell, Humans, hybrid, infrared spectroscopy, Magnetic Resonance Spectroscopy, materials testing, Membranes, Artificial, MG63 osteoblastic cells, Microscopy, Electron, Scanning, Neoplasm Proteins, Nuclear magnetic resonance spectroscopy, Organic-inorganic hybrid, osteoblast, Osteoblasts, Osteosarcoma, priority journal, Silanes, Silicates, siloxane, Spectroscopy, Fourier Transform Infrared, structure analysis, surface property, Synthesis (chemical), wettability, γ-Glycidoxypropyltrimethoxysilane (GPSM) |
| Chitosan-silicate hybrids were synthesized using γ- glycidoxypropyltrimethoxysilane (GPSM) as the agent for cross-linking the chitosan chains. CaCl 2 was introduced in the hybrids in expectation that it would improve cell adhesion and differentiation of the hybrid surfaces. Fourier-transform infrared (FT-IR) spectroscopy and
29Si CP-MAS NMR spectroscopy were used to analyze the structures of the hybrids. Cytocompatibility of the hybrids was investigated in terms of proliferation of an osteoblastic cell line, MG63. The adhesion and proliferation of the osteoblastic cells cultured on the surface of a chitosan-GPSM hybrid without calcium were similar to those on a control culture plate, and were better than those on a chitosan membrane. The ALP activity of the cells cultured on this hybrid was higher than that on the chitosan membrane. Contrary to expectations, the incorporation of calcium ions into the hybrids did not improve cell attachment and proliferation on their surfaces. © 2004 Elsevier Ltd. All rights reserved.
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