| Title | In vitro growth and differentiation of osteoblast-like human bone marrow cells on glass reinforced hydroxyapatite plasma-sprayed coatings |
| Publication Type | Journal Article |
| 1999 |
| Authors | Ferraz, MP, Fernandes, MH, Cabral, AT, Santos, JD, Monteiro, FJ |
| Journal | Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med. |
| Volume | 10 |
| Issue | 9Dordrecht, Netherlands |
| Pagination | 567 - 576 |
| Date Published | 1999/// |
| 09574530 (ISSN) |
| adult, alkaline phosphatase, article, Bone, bone marrow cell, Bone replacement materials, Calcium, calcium ion, case report, Cell culture, cell differentiation, cell growth, Cells, controlled study, culture medium, enzyme activity, Enzyme kinetics, glass, Growth kinetics, human, Human bone marrow cell, human cell, hydroxyapatite, male, osteoblast, Phosphates, phosphorus, priority journal, protein, protein determination, Proteins, scanning electron microscope, Scanning electron microscopy, Sprayed coatings |
| Human osteoblastic bone marrow cells were cultured far periods of up to 28 days in control conditions and on the surface of a glass reinforced hydroxyapatite composite (HA/G1) and commercial hydroxyapatite (HA) plasma-sprayed coatings, in the 'as-received' condition and after immersion treatment in culture medium for 21 days. Cultures were characterized for total protein content and alkaline phosphatase activity. Scanning electron microscope analyses were performed on control cultures, seeded materials and materials incubated in the absence of cells. Culture media were analyzed for total and ionized calcium and phosphorus concentrations throughout the incubation period. Immersion of HA/G1 and HA coatings in culture medium resulted in significant alterations to the levels of calcium and phosphorus in the medium, leading to surface modifications. However, seeded material samples showed significant differences in the pattern of variation of the levels of these species. Cell proliferation was observed in the 'as-received' HA/G1 composite, but cell mediated formation of mineral deposits was not proved. In contrast, 'as-received' HA hardly supported cell growth. Previously immersed material samples showed cell proliferation and evidence of biological formation of mineral deposits. However, the HA/G1 composite presented better surface characteristics for cell growth as the behavior of bone marrow eel Is was closer to that observed in control cultures.Human osteoblastic bone marrow cells were cultured for periods of up to 28 days in control conditions and on the surface of a glass reinforced hydroxyapatite composite (HA/G1) and commercial hydroxyapatite (HA) plasma-sprayed coatings, in the 'as-received' condition and after immersion treatment in culture medium for 21 days. Cultures were characterized for total protein content and alkaline phosphatase activity. Scanning electron microscope analyses were performed on control cultures, seeded materials and materials incubated in the absence of cells. Culture media were analyzed for total and ionized calcium and phosphorus concentrations throughout the incubation period. Immersion of HA/G1 and HA coatings in culture medium resulted in significant alterations to the levels of calcium and phosphorous in the medium, leading to surface modifications. However, seeded material samples showed significant differences in the pattern of variation of the levels of these species. Cell proliferation was observed in the 'as-received' HA/G1 composite, but cell mediated formation of mineral deposits was not proved. In contrast, 'as-received' HA hardly supported cell growth. Previously immersed material samples cell proliferation and evidence of biological formation of mineral deposits. However, the HA/G1 composite presented better surface characteristics for cell growth as the behavior of bone marrow cells was closer to that observed in control cultures.
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