| Title | In vitro immuno-cytotoxicity of iron evaluated by DNA synthesis of human T lymphocytes stimulated via CD2 and CD3 |
| Publication Type | Journal Article |
| 1993 |
| Authors | Carvalho, GS, Bravo, I |
| Journal | Journal of Materials Science: Materials in MedicineJ. MATER. SCI. MATER. MED. |
| Volume | 4 |
| Issue | 4 |
| Pagination | 366 - 371 |
| Date Published | 1993/// |
| 09574530 (ISSN) |
| article, Biochemistry, Biomedical engineering, cd2 antigen, cd3 antigen, cd8 antigen, Cell culture, cell stimulation, concentration response, DNA, DNA synthesis, erythrocyte, human, human cell, Human lymphocytes, Immuno-cytotoxicity, immunocytotoxicity, Iron, lymphocyte proliferation, monoclonal antibody, monocyte, Synthesis (chemical), t lymphocyte, toxicity |
| In previous studies it was demonstrated that the in vitro exposure of human lymphocytes to iron, nickel or cobalt salts causes a significant reduction of lymphocytes expressing CD2 and CD3 surface antigens. Since both molecules are involved in T lymphocyte activation, these studies suggest that the above metals might affect T-cell activation and proliferation. Thus a method was developed for the stimulation of lymphocytes in which both CD2 and CD3 molecules were triggered simultaneously. For this purpose an anti-CD3 monoclonal antibody (mAb) was chemically bound to human erythrocytes (HE), forming HEαCD3 conjugates, which were used for lymphocyte stimulation. In this work the effects of iron on lymphocyte proliferation was studied, following stimulation. |
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