| Title | In vitro study of the proliferation and growth of human bone marrow cells on apatite-wollastonite-2M glass ceramics |
| Publication Type | Journal Article |
| 2010 |
| Authors | Magallanes-Perdomo, M, De Aza, AH, Mateus, AY, Teixeira, S, Monteiro, FJ, De Aza, S, Pena, P |
| Journal | Acta BiomaterialiaActa Biomater. |
| Volume | 6 |
| Issue | 6 |
| Pagination | 2254 - 2263 |
| Date Published | 2010/// |
| 17427061 (ISSN) |
| Apatite, Apatites, article, Bioceramics, Biocompatibility, bone marrow cell, bone prosthesis, Bone Substitutes, Calcium Compounds, calcium derivative, calcium phosphate, calcium silicate, cell activity, cell adhesion, Cell culture, cell differentiation, cell enlargement, cell growth, cell proliferation, cell structure, cell viability, Cells, Cultured, ceramics, chemistry, confocal laser microscopy, crystal structure, Crystallization, cytology, glass, Glass ceramics, human, human cell, Humans, in vitro study, materials testing, Mesenchymal stem cell, Mesenchymal Stem Cells, methodology, osteoblast, Osteoblasts, physiology, priority journal, protein content, Scanning electron microscopy, silicate, Silicates, Surface Properties, surface property, Tissue regeneration, Wollastonite, X ray fluorescence |
| This study concerns the preparation and in vitro characterization of an apatite-wollastonite-2M bioactive glass ceramic which is intended to be used for the regeneration of hard tissue (i.e. in dental and craniomaxillofacial surgery). This bioglass ceramic has been obtained by appropriate thermal treatment through the devitrification (crystallization) of a glass with a stoichiometric eutectic composition within the Ca 3(PO
4)2-CaSiO
3 binary system. Crack-free specimens of the bioglass ceramic were immersed in human bone marrow cell cultures for 3, 7, 14 and 21 days, in order to study biocompatibility. Cell morphology, proliferation and colonization were assessed by scanning electron microscopy and confocal laser scanning microscopy. A total protein content assay was used to evaluate the viability and proliferation of cultured bone marrow cells. The results showed that the cells were able to adhere and proliferate on the designed material due to the essentiality of silicon and calcium as accessory factors for cell activity stimulation. © 2009 Acta Materialia Inc. All rights reserved.
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