INEB RECENT PUBLICATIONS: "A multi-compartment holder for spinner flasks improves expansion and osteogenic differentiation of mesenchymal stem cells in 3D scaffolds"

INEB researchers recently published an article in the Tissue Engineering, Part C Methods. The article is available since 20 of March 2014.

Title: A multi-compartment holder for spinner flasks improves expansion and osteogenic differentiation of mesenchymal stem cells in 3D scaffolds.
Authors: Graciosa Teixeira, Cristina Barrias CC, Ana Lourenço, Raquel Goncalves

In tissue engineering, spinner flasks are widely used as dynamic culture systems in suspension cell cultures. However, these culture systems are unsuitable to culture cells in three-dimensional (3D) scaffolds. In this study, a multi-compartment holder for 3D cell culture, easily adaptable to spinner flasks, was designed and validated with human mesenchymal stem cells (MSCs) culture in 3D porous chitosan scaffolds. This dynamic system significantly improved MSCs proliferation and spatial distribution within the 3D scaffold. Moreover, MSCs osteogenic differentiation was also improved. Overall, this study presents a novel culture system for 3D scaffolds that can broaden the field of application of spinner flasks.

IMAGE: Bioreactor 

MORE INFO: http://online.liebertpub.com/