RECENT PUBLICATIONS: Integrated Analysis of Biological Samples by Imaging Flow Cytometry

INEB researchers recently published an article in Microscopy and Microanalysis, available since 21st August 2015. The article is entitled "INEB researchers recently published an article in the Nanotechnology, 28(1): 26(31):315101, epub 2015 Jul 16. . The article is entitled "Antibacterial activity and biocompatibility of three-dimensional nanostructured porous granules of hydroxyapatite and zinc oxide nanoparticles—an in vitro and in vivo study" and is authored by Silva AM, Varela-Moreira A, Pereira Gomes C, Molinos M, Leite M, Almeida M, Ribeiro D, Schrader M, Figueiredo C, Barbosa M, Gonçalves R, Almeida C, Pêgo A, Santos SG, Gomez-Lazaro M.

Flow cytometry is a powerful technique that allows the acquisition of high amount of data in a relative short period. However this technique lacks the spatial resolution that a microscopic technique can achieve, which in turn is more time consuming. However, the combined advantages of these two techniques can be obtained when using an imaging flow cytometer. This equipment combines the statistical strength, sensitivity, and high speed of a conventional flow cytometer with the quantitative image analysis of the detailed cellular images with morphologic (and functional) information captured by a microscope. In the present work several examples of the use of the imaging flow cytometer where shown such as: quantification of a transcription factor nuclear translocation, characterization of cell secreted vesicles; assessment of nanoparticle internalization in drug delivery systems, identification and quantification of cell sub-populations based on complex morphological features, quantification of morphological changes in H. pylori, and organelle quantification.