RECENT PUBLICATIONS: A new approach for a blood-brain barrier model based on phospholipid vesicles: Membrane development and siRNA-loaded nanoparticles permeability

INEB researchers recently published an article in the Journal of Membrane Science, available since January 6th 2016. The article is entitled "A new approach for a blood-brain barrier model based on phospholipid vesicles: Membrane development and siRNA-loaded nanoparticles permeability" and is authored by Maria João Gomes, Jes Dreier, Jonathan Brewer, Susana Martins, Martin Brandl and Bruno Sarmento.

Developing innovative CNS treatments and improving the ability to cross BBB is urgent. Therefore, a BBB model where permeability experiments could be performed in a simple and fast way is crucially necessary to obtain high-throughput screening tools. This is where phospholipid vesicle-based permeability assay (PVPA) method opportunity arises. Through this technique, a non-cellular based BBB model able to assess passive transcellular-like permeability used as a tool for in vivo permeability prediction was developed based on phospholipid vesicles that mimic cellular membranes. Simultaneously, use of small interfering RNA (siRNA) to specifically silence efflux proteins present at BBB surface could be a useful advantage to circumvent BBB blockage. To improve siRNA delivery to brain and increase its ability to permeate BBB, lipid and polymeric nanoparticles were tested and their effect was observed by measuring siRNA permeability through the BBB-PVPA model.