



@article {2195,
	title = {Staphylococcus aureus and Staphylococcus epidermidis adhesion to nanohydroxyapatite in the presence of model proteins},
	journal = {Biomedical Materials (Bristol)Biomed. Mater.},
	volume = {7},
	year = {2012},
	note = {Export Date: 21 November 2012Source: ScopusArt. No.: 045010doi: 10.1088/1748-6041/7/4/045010Language of Original Document: EnglishCorrespondence Address: Ribeiro, M.; INEB-Instituto de Engenharia Biom{\'e}dica, Universidade Do Porto, Rua Campo Alegre, 823, 4150-180 Porto, Portugal; email: ribeiro_marta88@hotmail.comChemicals/CAS: fibronectin, 86088-83-7; human serum albumin, 9048-49-1; hydroxyapatite, 1306-06-5, 51198-94-8References: Darley, E.S.R., MacGowan, A.P., Antibiotic treatment of Gram-positive bone and joint infections (2004) Journal of Antimicrobial Chemotherapy, 53 (6), pp. 928-935. , DOI 10.1093/jac/dkh191;Lidgren, L., Knutson, K., Stef{\'a}nsd{\'o}ttir, A., Infection of prosthetic joints (2003) Best Pract. Res. Clin. Rheumatol., 17, pp. 209-218; 
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},
	month = {2012///},
	abstract = {Bacterial infections can have adverse effects on the efficacy, lifetime, and safety of an implanted device. The aim of this study was to investigate the initial adhesion of several strains, namely S. aureus and S. epidermidis, on two distinct types of nanohydroxyapatite (nanoHA), sintered at 725{\textdegree}C and 1000{\textdegree}C. A comparison was also made with nanohydroxyapatite having adsorbed fetal bovine serum (FBS), human fibronectin (FN) and human serum albumin (HSA). Adhered bacterial cells were examined by scanning electron microscopy and quantified as colony forming units after being released by sonication. The wettability of the sample surface with and without adsorbed protein was assessed by contact-angle measurements. NanoHA sintered at 1000{\textdegree}C showed lower bacterial adhesion than this heat-treated at 725{\textdegree}C. Adsorption of FBS onto the nanoHA surface caused a decrease in the adhesion of all strains on both materials. The bacterial adhesion patterns in the presence of FN were different for both nanoHA substrates; the adherence of the bacterial strains, except for the clinical strain of S.epidermidis, was significantly higher on nanoHA 1000 in comparison to nanoHA 1000 without protein and the bacterial adhesion on the FN-coated nanoHA 725 was lower in comparison to the bare nanoHA 725. The effect of HSA on bacterial adhesion was concentration and bacterial strain dependent. {\textcopyright} 2012 IOP Publishing Ltd.},
	keywords = {Adhesion, Adsorption, Adverse effect, article, Bacteria, Bacterial adhesion, bacterial cell, Bacterial cells, Bacterial infections, bacterial strain, Bacterial strains, bacterium adherence, Clinical strains, colony forming unit, Colony forming units, Contact angle, Contact-angle measurements, controlled study, Enzyme inhibition, Epidermidis, Fetal bovine serum, Fibronectin, heating, Human fibronectin, human serum albumin, Human serum albumins, hydroxyapatite, Implanted device, materials testing, Model proteins, Nano-HA, Nano-hydroxyapatite, Nanohydroxyapatite, nanoparticle, nonhuman, Proteins, S. aureus, Sample surface, Scanning electron microscopy, serum, Sintering, Staphylococcus aureus, Staphylococcus epidermidis, surface property, ultrasound, unclassified drug, wettability},
	isbn = {17486041 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84864391456\&partnerID=40\&md5=9170b87ade5223df5146d21981d073ef},
	author = {Ribeiro, M. and Monteiro, F.J. and Ferraz, M.P.}
}
