



@article {2484,
	title = {Biocompatibility of chemoenzymatically derived dextran-acrylate hydrogels},
	journal = {Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A},
	volume = {68},
	year = {2004},
	note = {Cited By (since 1996): 21Export Date: 21 November 2012Source: ScopusCODEN: JBMRCPubMed ID: 14762939Language of Original Document: EnglishCorrespondence Address: Dordick, J.S.; Department of Chemical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, United States; email: dordick@rpi.eduChemicals/CAS: acrylic acid, 10344-93-1, 79-10-7; dextran, 87915-38-6, 9014-78-2; Acrylates; Biocompatible Materials; Dextrans, 9004-54-0; Enzymes; HydrogelsReferences: Ferreira, L., Gil, M.H., Dordick, J.S., Enzymatic synthesis of dextran-containing hydrogels (2002) Biomaterials, 23, pp. 3957-3967;Peppas, N.A., Bures, P., Leobandung, W., Ichikawa, H., Hydrogels in pharmaceutical formulations (2000) Eur J Pharm Biopharm, 50, pp. 27-46; 
Park, K., Park, H., Biocompatibility issues of implantable drug delivery systems (1996) Pharm Res, 13 (12), pp. 1770-1776; 
Novick, S.J., Dordick, J.S., Preparation of active and stable biocatalytic hydrogels for use in selective transformation (1998) Chem Mater, 10 (4), pp. 955-958; 
Letourneur, D., Machy, D., Pell{\'e}, A., Marcon-Bachari, E., D{\'a}ngelo, G., Vogel, M., Chaubert, F., Michel, J.-B., Heparin and non-heparin-like dextrans differentially modulate endothelial cell proliferation: In vitro evaluation with soluble and crosslinked polysaccharide matrices (2002) J Biomed Mater Res, 60, pp. 94-100; 
{\"O}sterberg, E., Bergstr{\"o}m, K., Holmberg, K., Schuman, T.P., Riggs, J.A., Burns, N.L., Van Alstine, J.M., Harris, J.M., Protein-rejecting ability of surface-bound dextran in end-on and side-on configurations: Comparison to PEG (1995) J Biomed Mater Res, 29, pp. 741-747; 
Frazier, R.A., Matthijs, G., Davies, M.C., Roberts, C.J., Schacht, E., Tendler, S.J.B., Characterization of protein-resistant dextran monolayers (2000) Biomaterials, 21, pp. 957-966; 
Massia, S.P., Stark, J., Immobilized RGD peptides on surface-grafted dextran promote biospecific cell attachment (2001) J Biomed Mater Res, 56, pp. 390-399; 
Wong, W.H., Mooney, D.J., Synthesis and properties of biodegradable polymers used as synthetic matrices for tissue engineering (1997) Synthetic Biodegradable Polymer Scaffolds, p. 50. , Atala A, Mooney DJ, editors. Boston, MA: Birkh{\"a}user; 
Lee, K.Y., Mooney, D.J., Hydrogels for tissue engineering (2001) Chem Rev, 101 (7), pp. 1869-1879; 
Hern, D.L., Hubbell, J.A., Incorporation of adhesion peptides into nonadhesive hydrogels useful for tissue resurfacing (1998) J Biomed Mater Res, 39, pp. 266-276; 
Mann, B.K., Gobin, A.S., Tsai, A.T., Schmedlen, R.H., West, J.L., Smooth muscle cell growth in photopolymerized hydrogels with cell adhesive and proteolytically degradable domains: Synthetic ECM analogs for tissue engineering (2001) Biomaterials, 22, pp. 3045-3051; 
De Groot, C.J., Van Luyn, M.J.A., Van Dijk-Wolthuis, W.N.E., Cad{\'e}e, J.A., Plantinga, J.A., Den Otter, W., Hennink, W.E., In vitro biocompatibility of biodegradable dextran-based hydrogels tested with human fibroblasts (2001) Biomaterials, 22, pp. 1197-1203; 
Cad{\'e}e, J.A., Luyn, M.J.A., Brouwer, L.A., Plantinga, J.A., Van Wachem, P.B., Groot, C.J., Otter, W., Hennink, W.E., In vivo biocompatibility of dextran-based hydrogels (2000) J Biomed Mater Res, 50, pp. 397-404; 
Anderson, J.M., In vivo biocompatibility of implantable delivery systems and biomaterials (1994) Eur J Pharm Biopharm, 40 (1), pp. 1-8; 
Anderson, J.M., Inflammation, wound healing, and the foreign body reaction response (1996) Biomaterials Science, pp. 165-173. , Ratner BD, Hoffman AS, Schoen FJ, Lemons JE, editors. New York: Academic Press; 
(1992) ISO 10993-5: Biological Evaluation of Medical Devices. Test for Cytotoxicity: In Vitro Methods, , Geneva, Switzerland: International Organization for Standardization; 
Pizzoferato, A., Ciapetti, G., Stea, S., Cenni, E., Arciola, C.R., Granchi, D., Savarino, L., Cell culture methods for testing biocompatibility (1994) Clin Mater, 15, pp. 173-190; 
Bancroft, J.D., Stevens, A., (1996) Theory and Practice of Histological Techniques, pp. 99-211. , Edinburg: Churchill Livingstone; 
Mosmann, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays (1983) J Immunol Methods, 65, pp. 55-63; 
Moreau, M.F., Chappard, D., Lesourd, M., Month{\'e}ard, J.P., Basl{\'e}, M.F., Free radicals and side products released during methylmethacrylate are cytotoxic for osteoblastic cells (1998) J Biomed Mater Res, 40, pp. 124-131; 
Black, J., The inflammatory response (1999) Biological Performance of Materials, p. 131. , Black J, editor. New York: Marcel Dekker; 
Murch, A.R., Grounds, M.D., Marshall, C.A., Papadimitriou, J.M., Direct evidence that inflammatory multinucleate giant cells form by fusion (1982) J Pathol, 137, pp. 177-180; 
Kuijpers, A.J., Wachem, P.B., Luyn, M.J.A., Plantinga, J.A., Engbers, G.H.M., Krijgsveld, J., Zaat, S.A.J., Feijen, J., In vivo compatibility and degradation of crosslinked gelatin gels incorporated in knitted Dacron (2000) J Biomed Mater Res, 51, pp. 136-145; 
Kuijpers, A.J., Van Wachem, P.B., Luyn, M.J.A., Brouwer, L.A., Engbers, G.H.M., Krijgsveld, J., Zaat, S.A.J., Feijen, J., In vitro and in vivo evaluation of gelatin-chondroitin sulphate hydrogels for controlled release of antibacterial proteins (2000) Biomaterials, 21, pp. 1763-1772; 
Hooper, K.A., Macon, N.D., Kohn, J., Comparative histological evaluation of new tyrosine-derived polymers and poly(L-lactic acid) as a function of polymer degradation (1998) J Biomed Mater Res, 41, pp. 443-454; 
Bos, R.R.M., Rozema, F.R., Boering, G., Nijenhuis, A.J., Pennings, A.J., Verwey, A.B., Nieuwenhuis, P., Jansen, H.W.B., Degradation of and tissue reaction to biodegradable poly(L-lactide) for use as internal fixation of fractures: A study in rats (1991) Biomaterials, 12, pp. 32-36; 
Nakamura, T., Veda, H., Tsuda, T., Li, Y.-H., Kiyotani, T., Inoue, M., Matsumoto, K., Shimizu, Y., Long-term implantation test and tumorigenicity of polyvinyl alcohol hydrogel plates (2001) J Biomed Mater Res, 56, pp. 289-296; 
Dziubla, T.D., Torjman, M.C., Joseph, J.I., Murphy-Tatum, M., Lowman, A.M., Evaluation of porous networks of poly(2-hydroxyethylmethacrylate) as interfacial drug delivery devices (2001) Biomaterials, 22, pp. 2893-2899; 
Spector, M., Lalor, P.A., In vivo assessment of tissue compatibility (1996) Biomaterials Science, pp. 220-228. , Ratner BD, Hoffman AS, Schoen FJ, Lemons JE, editors. New York: Academic Press
},
	month = {2004///},
	pages = {584 - 596},
	abstract = {The biocompatibility of chemoenzymatically generated dextran-acrylate hydrogels has been evaluated in vitro, using human foreskin fibroblasts, and in vivo, by subcutaneous and intramuscular implantation in Wistar rats for up to 40 days. In vitro tests show that hydrogel extracts only minimally reduced (<10\%) the mitochondrial metabolic activity of fibroblasts. Direct contact of the hydrogels with cells induced a cellular proliferation inhibition index (CPII) of 50-80\%, compared with a control, whereas through indirect contact, the CPII values were <16\%, suggesting that the high CPII values achieved in the direct assay test were likely due to mechanical stress or limitations in oxygen diffusion. Hence, the hydrogels were noncytotoxic. Moreover, cell-material interaction studies show that these hydrogels were nonadhesive. Finally, histologic evaluation of tissue response to subcutaneous and intramuscular implants showed acceptable levels of biocompatibility, as characterized by a normal cellular response and the absence of necrosis of the surrounding tissues of the implant. In the first 10 days, the foreign-body reaction in the intramuscular implantation was more severe than in subcutaneous implantation, becoming identical after 30 days. In both cases, dextran hydrogels did not show signs of degradation 6 weeks postimplantation and were surrounded by a thin fibrous capsule and some macrophages and giant cells. This response is typical with a number of nondegradable biocompatible materials. These results indicate that dextran hydrogels are biocompatible, and may have suitable applications as implantable long-term peptide/protein delivery systems or scaffolds for tissue engineering. {\textcopyright} 2004 Wiley Periodicals, Inc.},
	keywords = {Acrylates, acrylic acid, animal experiment, animal model, animal tissue, Animals, article, Biocatalytic polyester, Biocompatibility, Biocompatible Materials, Biodegradation, Environmental, Biodiversity, Biomaterials, cell adhesion, Cell Division, cell interaction, cell proliferation, Cells, Cultured, Coenzymes, controlled study, Cytotoxicity, dextran, dextran acrylate, dextran acrylate hydrogel, dextran hydrogel, Dextrans, drug delivery system, Enzymes, fibroblast, Fibroblasts, Fibrous capsules, foreign body reaction, Foreign-Body Reaction, giant cell, human, human cell, Humans, hydrogel, Hydrogels, implantation, Implants (surgical), Implants, Experimental, in vitro study, in vivo study, macrophage, male, materials testing, mechanical stress, Mechanical stresses, metabolism, Mitochondria, mitochondrial respiration, nonhuman, oxygen diffusion, prepuce, rat, Rats, Rats, Wistar, Tissue, Tissue engineering, unclassified drug},
	isbn = {00219304 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-1342280525\&partnerID=40\&md5=fe5529e53db4261e72bde7a75e2cf2cc},
	author = {Ferreira, L. and Rafael, A. and Lamghari, M. and Barbosa, M.A. and Gil, M.H. and Cabrita, A.M.S. and Dordick, J.S.}
}
