



@article {2509,
	title = {Albumin adsorption on Cibacron Blue F3G-A immobilized onto oligo(ethylene glycol)-terminated self-assembled monolayers},
	journal = {Journal of Materials Science: Materials in MedicineJ. Mater. Sci. Mater. Med.},
	volume = {14},
	year = {2003},
	note = {Cited By (since 1996): 20Export Date: 21 November 2012Source: ScopusCODEN: JSMMEdoi: 10.1023/A:1026394431100Language of Original Document: EnglishCorrespondence Address: Martins, Ma.C.L.; INEB - Inst. de Engenharia Biomedica, Laborat{\'o}rio de Biomateriais, Rua do Campo Alegre, 823, 4150-180 Porto, Portugal; email: cmartins@ibmc.up.ptChemicals/CAS: cibacron blue f3ga, 12236-82-7, 53127-08-5; ethylene glycol, 107-21-1; fibrinogen, 9001-32-5; gold, 7440-57-5; human serum albumin, 9048-49-1; iodine 125, 14158-31-7, 22822-81-7; macrogol, 25322-68-3; thiol derivative, 13940-21-1; water, 7732-18-5References: Ratner, B.D., (1996) J. Mol. Recognit., 9, p. 617;Kottke-Marchant, K., Anderson, J.M., Umemura, Y., Marchant, R.E., (1998) Biomaterials, 10, p. 147; 
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},
	month = {2003///},
	pages = {945 - 954},
	abstract = {Self-assembled monolayers can be tailored with specific ligands to a certain protein and at the same time prevent the non-specific adsorption of other proteins. Cibacron Blue F3G-A (CB-thiol) was successfully immobilized onto tetra(ethylene glycol)-terminated alkanethiol (CB-thiol). The affinity of human serum albumin (HSA) to immobilized Cibacron Blue F3G-A was studied using mixed thiolate self-assembled monolayers on gold with different n-alkyl chain lengths and functional terminal groups (CH                        3-; OH- and tetra(ethylene glycol)). Surfaces were characterized using X-ray photoelectron spectroscopy and water contact angle measurements. Albumin adsorption and exchangeability of the adsorbed albumin molecules with other albumin molecules in solution were evaluated using 
                        125I-radiolabeled HSA. Competitive adsorption between albumin and fibrinogen to the different self-assembled monolayers (SAMs) was also investigated. Results showed that the incorporation of CB-thiol on the monolayers does not increase the HSA adsorption and reversibility on the SAMs. However, although specific adsorption of HSA to the immobilized Cibacron Blue F3G-A was not demonstrated, the presence of CB-thiol decreases the affinity of fibrinogen to the OH-terminated SAMs. {\textcopyright} 2003 Kluwer Academic Publishers.
},
	keywords = {Adsorption, Albumin, aqueous solution, article, Cell immobilization, cibacron blue f3ga, Contact angle, controlled study, Drug products, Ethylene Glycol, fibrinogen, functional group, Glycols, gold, human, human serum albumin, iodine 125, isotope labeling, ligand, macrogol, molecule, priority journal, protein assembly, protein binding, protein immobilization, protein structure, Proteins, radioassay, Self assembly, surface property, synthesis, tetra(ethylene glycol) undecanethiol, thiol, thiol derivative, unclassified drug, Water, X ray photoelectron spectroscopy},
	isbn = {09574530 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0344629191\&partnerID=40\&md5=6ebb3020ea2ade3febc3906ce0672cc1},
	author = {Martins, Ma.C.L. and Naeemi, E. and Ratner, B.D. and Barbosa, M.A.}
}
