



@article {3275,
	title = {Biodistribution and pharmacokinetics of Dapivirine-loaded nanoparticles after vaginal delivery in mice},
	journal = {Pharmaceutical Research},
	volume = {31},
	year = {2014},
	note = {Cited By (since 1996):2Export Date: 21 November 2014},
	month = {2014},
	pages = {1834 - 1845},
	abstract = {Purpose: To assess the potential of polymeric nanoparticles (NPs) to affect the genital distribution and local and systemic pharmacokinetics (PK) of the anti-HIV microbicide drug candidate dapivirine after vaginal delivery. Methods: Dapivirine-loaded, poly(ethylene oxide)-coated poly(epsilon-caprolactone) (PEO-PCL) NPs were prepared by a nanoprecipitation method. Genital distribution of NPs and their ability to modify the PK of dapivirine up to 24 h was assessed after vaginal instillation in a female mouse model. Also, the safety of NPs upon daily administration for 14 days was assessed by histological analysis and chemokine/cytokine content in vaginal lavages. Results: PEO-PCL NPs (180-200 nm) were rapidly eliminated after administration but able to distribute throughout the vagina and lower uterus, and capable of tackling mucus and penetrate the epithelial lining. Nanocarriers modified the PK of dapivirine, with higher drug levels being recovered from vaginal lavages and vaginal/lower uterine tissues as compared to a drug suspension. Systemic drug exposure was reduced when NPs were used. Also, NPs were shown safe upon administration for 14 days. Conclusions: Dapivirine-loaded PEO-PCL NPs were able to provide likely favorable genital drug levels, thus attesting the potential value of using this vaginal drug delivery nanosystem in the context of HIV prophylaxis. {\textcopyright} 2014 Springer Science+Business Media New York.},
	keywords = {HIV/AIDS, microbicides, poly(ε-caprolactone), pre-exposure prophylaxis, vaginal drug delivery},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84904252215\&partnerID=40\&md5=e50091fa44bc3ac794251baa9e57d736},
	author = {Das Neves, J. and Ara{\'u}jo, F. and Andrade, F. and Amiji, M. and Bahia, M.F. and Sarmento, B.}
}
@article {3286,
	title = {Nanoparticle-based drug delivery to improve the efficacy of antiretroviral therapy in the central nervous system},
	journal = {International Journal of Nanomedicine},
	volume = {9},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {1757 - 1769},
	abstract = {Antiretroviral drug therapy plays a cornerstone role in the treatment of human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome patients. Despite obvious advances over the past 3 decades, new approaches toward improved management of infected individuals are still required. Drug distribution to the central nervous system (CNS) is required in order to limit and control viral infection, but the presence of natural barrier structures, in particular the blood-brain barrier, strongly limits the perfusion of anti-HIV compounds into this anatomical site. Nanotechnology-based approaches may help providing solutions for antiretro-viral drug delivery to the CNS by potentially prolonging systemic drug circulation, increasing the crossing and reducing the efflux of active compounds at the blood-brain barrier, and providing cell/tissue-targeting and intracellular drug delivery. After an initial overview on the basic features of HIV infection of the CNS and barriers to active compound delivery to this anatomical site, this review focuses on recent strategies based on antiretroviral drug-loaded solid nanoparticles and drug nanosuspensions for the potential management of HIV infection of the CNS. {\textcopyright} 2014 Gomes et al.},
	keywords = {Blood-brain barrier, drug targeting, Efflux transporters, HIV/AIDS, Protease inhibitors},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84898486385\&partnerID=40\&md5=31d2c7f6e0759fb62143e329fb8599ae},
	author = {Gomes, M.J. and Das Neves, J. and Sarmento, B.}
}
@article {3309,
	title = {What do Portuguese women prefer regarding vaginal products? Results from a cross-sectional web-based survey},
	journal = {Pharmaceutics},
	volume = {6},
	year = {2014},
	note = {Export Date: 21 November 2014},
	month = {2014},
	pages = {543 - 556},
	abstract = {Therapeutic outcomes of vaginal products depend not only on their ability to deliver drugs to or through the vagina but also on acceptability and correct use. Women{\textquoteright}s preferences, in turn, may vary according to age and cultural backgrounds. In this work, an anonymous online survey was completed by 2529 Portuguese women to assess their preferences for physical characteristics and mode of application of vaginal products, according to age. Additionally, intention to use and misconceptions about these issues were assessed. The majority of women of all age groups would use vaginal products to treat or prevent diseases, upon medical prescription. Women preferred vaginal products to be odorless and colorless gels, creams and ointments composed by natural origin drugs/excipients and applied by means of an applicator. Although the majority of women would prefer not to insert any product in the vagina, intention to use for self and recommendation to use for others was associated with previous experiences with vaginal products. General concerns and misconceptions related to use of vaginal products were rare. These data may contribute to the development of products that women are more prone to use.},
	keywords = {Acceptability, Vaginal dosage forms, Vaginal products, Women{\textquoteright}s preferences},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84908210757\&partnerID=40\&md5=5515e40e97ff157b0a5b4498557008ef},
	author = {Palmeira-de-Oliveira, R. and Duarte, P. and Palmeira-de-Oliveira, A. and Das Neves, J. and Amaral, M.H. and Breitenfeld, L. and Martinez-de-Oliveira, J.}
}
@article {2846,
	title = {Assessing the physical-chemical properties and stability of dapivirine-loaded polymeric nanoparticles},
	journal = {International Journal of Pharmaceutics},
	volume = {456},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {307 - 314},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84884308158\&partnerID=40\&md5=52f91c19cbe922064bbdc786298301a0},
	author = {Das Neves, J. and Amiji, M. and Bahia, M.F. and Sarmento, B.}
}
@article {2863,
	title = {Hydrolyzed galactomannan-modified nanoparticles and flower-like polymeric micelles for the active targeting of rifampicin to macrophages},
	journal = {Journal of Biomedical Nanotechnology},
	volume = {9},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {1076 - 1087},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84877815633\&partnerID=40\&md5=d4bbcc67f9930d53201ec08dbcfce826},
	author = {Moretton, M.A. and Chiappetta, D.A. and Andrade, F. and Das Neves, J. and Ferreira, D. and Sarmento, B. and Sosnik, A.}
}
@article {2857,
	title = {In vitro and Ex Vivo evaluation of polymeric nanoparticles for vaginal and rectal delivery of the anti-HIV drug dapivirine},
	journal = {Molecular Pharmaceutics},
	volume = {10},
	year = {2013},
	note = {Cited By (since 1996):1Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {2793 - 2807},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84879736868\&partnerID=40\&md5=cd7771212c94b7031331e338743b05ed},
	author = {Das Neves, J. and Ara{\'u}jo, F. and Andrade, F. and Michiels, J. and Ari{\"e}n, K.K. and Vanham, G. and Amiji, M. and Bahia, M.F. and Sarmento, B.}
}
@article {2853,
	title = {Medicago spp. extracts as promising ingredients for skin care products},
	journal = {Industrial Crops and Products},
	volume = {49},
	year = {2013},
	note = {Export Date: 12 December 2013Source: Scopus},
	month = {2013///},
	pages = {634 - 644},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84880378375\&partnerID=40\&md5=1b60aaf1efb444f653b44800529f5ae3},
	author = {Rodrigues, F. and Palmeira-de-Oliveira, A. and Das Neves, J. and Sarmento, B. and Amaral, M.H. and Oliveira, M.B.}
}
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	title = {Cell-based in vitro models for predicting drug permeability},
	journal = {Expert Opinion on Drug Metabolism and ToxicologyExpert Opin. Drug Metab. Toxicol.},
	volume = {8},
	year = {2012},
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},
	month = {2012///},
	pages = {607 - 621},
	abstract = {In vitro cell models have been used to predict drug permeation in early stages of drug development, since they represent an easy and reproducible method, allowing the tracking of drug absorption rate and mechanism, with an advantageous costbenefit ratio. Such cell-based models are mainly composed of immortalized cells with an intrinsic ability to grow in a monolayer when seeded in permeable supports, maintaining their physiologic characteristics regarding epithelium cell physiology and functionality. Areas covered: This review summarizes the most important intestinal, pulmonary, nasal, vaginal, rectal, ocular and skin cell-based in vitro models for predicting the permeability of drugs. Moreover, the similitude between in vitro cell models and in vivo conditions are discussed, providing evidence that each model may provisionally resemble different drug absorption route. Expert opinion: Despite the widespread use of in vitro cell models for drug permeability and absorption evaluation purposes, a detailed study on the properties of these models and their in vitroin vivo correlation compared with human data are required to further use in order to consider a future drug discovery optimization and clinical development. {\textcopyright} 2012 Informa UK, Ltd.},
	keywords = {Animals, Caco-2 Cells, carcinoma cell, cell differentiation, cell growth, Cell Line, Cell Membrane Permeability, cell models, cell proliferation, cell strain CACO 2, cell type, Cells, conjunctiva, drug absorption, drug bioavailability, drug delivery system, drug penetration, drug screening, drug transport, epithelium cell, Eye, human, Humans, in vitro models, in vitro study, in vitroin vivo correlation, Intestinal Absorption, intestinal mucosa, intestine cell, Intestines, Lung, lung alveolus cell, Models, Biological, molecular weight, nasal cell, nonhuman, ocular cell, permeability, Pharmaceutical Preparations, rectal cell, review, Skin, skin cell, vaginal cell},
	isbn = {17425255 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84859967380\&partnerID=40\&md5=f531a0a6e96c96e8daf71a6b36dbec8a},
	author = {Sarmento, B. and Andrade, F. and Da Silva, S.B. and Rodrigues, F. and Das Neves, J. and Ferreira, D.}
}
@article {2181,
	title = {Interactions of microbicide nanoparticles with a simulated vaginal fluid},
	journal = {Molecular PharmaceuticsMol. Pharm.},
	volume = {9},
	year = {2012},
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},
	month = {2012///},
	pages = {3347 - 3356},
	abstract = {The interaction with cervicovaginal mucus presents the potential to impact the performance of drug nanocarriers. These systems must migrate through this biological fluid in order to deliver their drug payload to the underlying mucosal surface. We studied the ability of dapivirine-loaded polycaprolactone (PCL)-based nanoparticles (NPs) to interact with a simulated vaginal fluid (SVF) incorporating mucin. Different surface modifiers were used to produce NPs with either negative (poloxamer 338 NF and sodium lauryl sulfate) or positive (cetyltrimethylammonium bromide) surface charge. Studies were performed using the mucin particle method, rheological measurements, and real-time multiple particle tracking. Results showed that SVF presented rheological properties similar to those of human cervicovaginal mucus. Analysis of NP transport indicated mild interactions with mucin and low adhesive potential. In general, negatively charged NPs underwent subdiffusive transport in SVF, i.e., hindered as compared to their diffusion in water, but faster than for positively charged NPs. These differences were increased when the pH of SVF was changed from 4.2 to 7.0. Diffusivity was 50- and 172-fold lower in SVF at pH 4.2 than in water for negatively charged and positively charged NPs, respectively. At pH 7.0, this decrease was around 20- and 385-fold, respectively. The estimated times required to cross a layer of SVF were equal to or lower than 1.7 h for negatively charged NPs, while for positively charged NPs these values were equal to or higher than 7 h. Overall, our results suggest that negatively charged PCL NPs may be suitable to be used as carriers in order to deliver dapivirine and potentially other antiretroviral drugs to the cervicovaginal mucosal lining. Also, they further reinforce the importance in characterizing the interactions of nanosystems with mucus fluids or surrogates when considering mucosal drug delivery. {\textcopyright} 2012 American Chemical Society.},
	keywords = {microbicides, mucin, mucoadhesion, mucosal drug delivery, particle tracking, rheology},
	isbn = {15438384 (ISSN)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84868526145\&partnerID=40\&md5=bd161f1b2ce30fba31c7a482dbe60b86},
	author = {Das Neves, J. and Rocha, C.M.R. and Gon{\c c}alves, M.P. and Carrier, R.L. and Amiji, M. and Bahia, M.F. and Sarmento, B.}
}
