| Title | Inflammatory responses and cell adhesion to self-assembled monolayers of alkanethiolates on gold |
| Publication Type | Journal Article |
| 2004 |
| Authors | Barbosa, JN, Barbosa, MAA, Águas, AP |
| Journal | BiomaterialsBiomaterials |
| Volume | 25 |
| Issue | 13 |
| Pagination | 2557 - 2563 |
| Date Published | 2004/// |
| 01429612 (ISSN) |
| Air-pouch model, alkane derivative, animal cell, animal experiment, animal model, Animals, article, Biomaterials, cell adhesion, Cell culture, cell density, cell surface, Cell-surface interactions, controlled study, exudate, gold, gold complex, hydrophobicity, implantation, Inflammation, Inflammatory responses, leukocyte, Leukocytes, male, material coating, Mice, Mice, Inbred BALB C, Microscopy, Electron, Scanning, mouse, neutrophil, nonhuman, polymorphonuclear cell, priority journal, Rodentia, Scanning electron microscopy, Self assembly, Self-assembled monolayers, Sulfhydryl Compounds, Surface chemistry, surface property |
| The acute inflammatory response and the adhesion of cells to self-assembled monolayers (SAMs) of well-defined surface chemistry was studied in vivo using a rodent air-pouch model of inflammation. SAMs with three different terminal functional groups (OH, COOH and CH 3) were implanted in subcutaneous air pouches induced in BALB/c mice. After 24h, inflammatory cells were recovered from the air pouches and the implants were removed and prepared for observation by scanning electron microscopy (SEM). The implants coated with OH and CH
3, were found to cause the highest recruitment of inflammatory cells into the subcutaneous pouches. Polymorphonuclear neutrophils (PMNs) leukocytes predominated over mononuclear cells in inflammatory exudates of SAMs-coated implants, the opposite being found in uncoated implants (controls). CH
3-coated implants induced the highest number of inflammatory cells and also the largest percentage of PMNs seen in the subcutaneous pouches. Control and OH-covered implants presented the higher densities of attached inflammatory cells detected by SEM. In contrast, the CH
3-coated implants showed a very low density of cells adherent to the implant surface. We conclude that the chemical nature and the degree of hydrophobicity of the surface of implants modulate both the local acute inflammatory reaction and the adhesion of leukocytes. © 2003 Elsevier Ltd. All rights reserved.
|
| http://www.scopus.com/inward/record.url?eid=2-s2.0-0742324843&partnerID=40&md5=4fc63f7a8a6d1c445f0be27356884543 |
