| Title | Human osteoblast response to silicon-substituted hydroxyapatite |
| Publication Type | Journal Article |
| 2006 |
| Authors | Botelho, CM, Brooks, RA, Best, SM, Lopes, MA, Santos, JD, Rushton, N, Bonfield, W |
| Journal | Journal of Biomedical Materials Research - Part AJ. Biomed. Mater. Res. Part A |
| Volume | 79 |
| Issue | 3 |
| Pagination | 723 - 730 |
| Date Published | 2006/// |
| 00219304 (ISSN) |
| alkaline phosphatase, article, Biomarkers, bone mineral, cell activity, Cell culture, cell density, cell differentiation, cell function, cell lysate, cell marker, cell protein, Cells, Cells, Cultured, collagen type 1, Collagen type I, culture medium, Durapatite, Fluorescence microscopy, glycerol 2 phosphate, hormone determination, human, human cell, Human osteoblasts, Humans, Hydrocortisone, hydroxyapatite, in vitro study, Minerals, Nodular mineral formation, osteoblast, Osteoblasts, osteocalcin, Osteocalcin (OC), phenotype, Phosphoric Diester Hydrolases, protein analysis, protein synthesis, Pyrophosphatases, Silicon, Substitution reactions, Substrates, Tetracycline, β-glycerophosphate |
| Human osteoblasts were cultured on hydroxyapatite (HA), 0.8 wt % silicon substituted hydroxyapatite (Si-HA) and 1.5 wt % Si-HA discs. The influence of these substrates on cell behaviour in vitro was assessed by measuring total protein in the cell lysate and the production of several phenotypic markers: collagen type I (COL 1), alkaline phosphatase (ALP), osteocalcin (OC), and the formation of bone mineral. After 7 days, β-glycerophosphate and physiological levels of hydrocortisone were added to the culture medium to stimulate cell differentiation and mineral production. There was a significantly higher production of ALP on 1.5 wt % Si-HA at day 7 following which, the addition of hydrocortisone promoted the differentiation of cells on the other two substrates. Hydrocortisone addition also decreased the production of OC. During the period, when hydrocortisone was present, no significant difference in behavior was seen between cells on Si-HA and HA; however, following removal of hydrocortisone, cells responded to 0.8 wt % Si-HA with a significant increase in protein production. Using fluorescence microscopy, nodular structures labeled with tetracycline were observed on the surface of all substrates after 21 days. These structures were deposited on areas of high cell density but were not related to the presence or level of silicon in the substrate. These results indicate that human osteoblasts are affected by the presence of silicon in the HA substrate and that the timing of these effects may be dependent upon the level of silicon substitution. © 2006 Wiley Periodicals, Inc. |
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