| Title | Synthesis and cytocompatibility of porous chitosan-silicate hybrids for tissue engineering scaffold application |
| Publication Type | Journal Article |
| 2008 |
| Authors | Shirosaki, Y, Okayama, T, Tsuru, K, Hayakawa, S, Osaka, A |
| Journal | Chemical Engineering JournalChem. Eng. J. |
| Volume | 137 |
| Issue | 1 |
| Pagination | 122 - 128 |
| Date Published | 2008/// |
| 13858947 (ISSN) |
| chitosan, Cytocompatibility, GPTMS, Organic-inorganic hybrid, Osteoblastic cell MG63, Osteoblasts, Pore size, Pore structure, Scaffold, Silicates, Tissue engineering, γ-Glycidoxypropyltrimethoxysilane (GPTMS) |
| Chitosan-silicate hybrids with 3D porous structures were prepared with freeze-drying precursor solutions derived from chitosan and γ-glycidoxypropyltrimethoxysilane (GPTMS). They were formed easily in any shape, such as sheets, pellets, disks, granules, and even roll-cakes. The pore size was strongly dependent on the freezing temperature: lower freezing temperature resulted smaller pores, about 110 μm for the hybrids frozen at -20 °C, and about 50 μm for those at -85 °C. The pore size was little dependent on the GPTMS content. In contrast, the GPTMS content affected porosity a littlie: ∼80% for chitosan, and ∼90% for the GPTMS-containing hybrids. Thus, their porous microstructure was controllable due to the freezing temperature and composition. MG63 osteoblastic cells were cultured up to 7 days on the porous hybrids. The cells adhered on the pore walls, proliferated, and migrated deep into the pore structure. It was thus concluded that the present chitosan-silicate hybrids were promising for tissue engineering scaffold applications. © 2007 Elsevier B.V. All rights reserved. |
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